Rw1G004680

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
8987915 .. 8992325
4411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004680.1

Sequence Viewer

Length: 3696 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTGTTCCTAAATTTCAGGGGGGAAGATACGCGCCATCGTTATATCCATTATCTGTATCATGCACTAACTCAGAGAGGAATTCTCACCTTTATGGATGACCGAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAAGTGCAATTGAGGAGTCTAGATCTGCGATTGTCATTCTCTCACGGAACTATGCTTCTTCATCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATAGGCCAACAAGTCCTCCCCGTCTTCTTCTACGGCGTGGATCCTTCTGATGTGCGGCACCAAACGGGAAATTTTGAGCTCAAAGTAAGGGAACTCCAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATACTGAGGGCAGACTAAATGCCTGGAGAGCTGCTTTGACTGAGGTCGCCAGTCTTTCTGGCTGGGATTACAAGAAGTATTCATATCAAAGAACAAAAATAATTGACGAAATTGTTAATCATATACTAAAGAAGTCCATGTATACATCTTCAAGTGTTGCCAAGGGCTTCATAGGAATGGATTCCCGCATTCATGATTTATTAAGCAATTACATATGTCCGCAGCTTGATGAGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCCCGAGCTATCTATGATGAAATTTGTCAGAATTTTGACCGAACCTGCTTTCTTCCCAATGTTAGAGAACTGTCTAAAAACAATGGCCTAGTTTCTCTCCAAGAAAAACTTCTTTCCAAAATCCTGATGGCAAAAATTAAAAATATAGAGGATGAATACGCAGGAGCTGCTATGATTCAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCACAAGTGGTTTGGTCGAGGGAGTAGAATTGTCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACGGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATTCCCCCCACCAGAAGATTATTTGGATCTGTGCTACCATATTATAGGGTATGCTAAGGGGCTTCCCTTGGCTCTCGTGGTTTTAGGTTATTTTCTATTTGGTAGAAGAACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGACAAACGCATTATTGATGTGCTTCGGATTAGTTTTGATGGGTTGGATGAAAAAGACAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGGTAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAATGCACTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCGTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAAAAATATGGGAACAGATTCAATCCAAGGCATGGTAATAGAGTTGACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTCTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAACGTGGACCTTCCCAAAGGCCTCACCTGTCTTTCTAATTCCTTGAGACTTCTTGAATGGACTGGGTGCCCCTTAAGATCTCTCCCACAAAACTTTGATGCAGATGAACTTGTTGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTAAAGTTCATCAAACTCTGCCATTCTCCAAACATTGTGGAGACCCCAGACCTCGCAGGTGTTCAAAATCTTGAGAGTTTAGATCTTGAAGGATGTTCTCGTTTGGTAAGAATCCATCAATCCCTTGCATTTCTCAAAAAACTTATTGTGCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAGTCTCTTGAATCATTAATTCTTTCTAACTGTTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAATGTTTGTTGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTACGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAATTGAGGTCTATTAAAGATCTTAATCTTTCTGGATGCTTGAAACTGGGCAAACCTCAGGTAAATGTGGGGGTGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGTGTCTACAATAGAAATGTCATCTACCCATGATCACAAGAAAGAAACTTATGTGAATAGTGGGTCCGCAATAGAAATGTCGTCTACCCATGATCGCAAGAAAAAAGGGAGAGGTTCAATCTTTCAAGGATGCAAAGTAGTTTGGCGATCTTTCAAAAAGTTCTTGCCTTCTGGATTGGTGCTAGAAGTGAATACAGAGCCAAAGAGCTTCCACTCGCCTATATCACAAAACGTGAATGCAGAGCTAAAGAGCTTCCCCTTGTCTGTATCTGGTCTGTGTAATTTAATAAATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGGAACAATTTTGTTCATCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAGATCATATTTCAATTTCATCAATTGCTTCAATCTAAACAGCAATCAAGGATGCAATGACATAGCATTCAAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAGCAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCTTGAGTGGTTCAGCCATCAGAGTGTAGGGTGTTCTGTAAGTGTATCTCTACCTGCACATTGGAATAACAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTGCATGAGCACCATCAGGTGGATGAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTTGATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAATCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATGTGCAAGAGTTCAACCAAACAACCACTCAATCAAGCAGTGGGATGTCTCCTTATGAGGATATGCTGATTGGTTTTGACAATCCAGTTGCAGGGGAAACCAGTGGCAGTGGTGGCGGAACTTGCACGCTTGAAGAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1231

Amino Acids

139.82

Weight (kDa)

6.73

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 193 2e-49 TIR domain
TIR_2 PF13676 14 - 108 1.7e-13 TIR domain
NB-ARC PF00931 218 - 373 1.1e-19 NB-ARC domain
NACHT PF05729 223 - 320 2.5e-06 NACHT domain
WHD_ROQ1 PF23282 440 - 511 3.5e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 609 - 627 5.2e-07 Leucine Rich Repeat
LRR_14 PF23598 677 - 779 1.4e-09 Leucine-rich repeat region
LRR_13 PF23286 700 - 784 4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1038 - 1187 1.5e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3694
AarI CACCTGC 1 cut(s) 1940
Acc36I ACCTGC 3 cut(s) 761, 1940, 3190
AccB1I GGYRCC 2 cut(s) 345, 1782
AccB7I CCANNNNNTGG 2 cut(s) 1627, 3256
AccI GTMKAC 4 cut(s) 566, 2417, 2495, 2905
AccII CGCG 1 cut(s) 70
AciI CCGC 7 cut(s) 343, 610, 644, 685, 892, 2478, 3669
AclWI GGATC 7 cut(s) 323, 336, 667, 680, 942, 1152, 1386
AcuI CTGAAG 5 cut(s) 1613, 2799, 3077, 3341, 3533
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 2932, 3237, 3448
AfiI CCNNNNNNNGG 4 cut(s) 1424, 1627, 3256, 3644
AflII CTTAAG 1 cut(s) 1789
AjnI CCWGG 4 cut(s) 446, 1543, 3115, 3505
AjuI GAANNNNNNNTTGG 4 cut(s) 801, 833, 2837, 2869
Alw21I GWGCWC 2 cut(s) 369, 3249
AlwI GGATC 7 cut(s) 323, 336, 667, 680, 942, 1152, 1386
AlwNI CAGNNNCTG 1 cut(s) 875
Ama87I CYCGRG 3 cut(s) 711, 2069, 2150
AoxI GGCC 6 cut(s) 167, 292, 793, 1735, 2242, 3503
ApeKI GCWGC 3 cut(s) 455, 646, 875
AseI ATTAAT 1 cut(s) 2114
Asp700I GAANNNNTTC 7 cut(s) 604, 816, 1086, 1518, 1612, 3062, 3099
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 3 cut(s) 1723, 2475, 3503
AsuHPI GGTGA 8 cut(s) 115, 1088, 1732, 2389, 2741, 2785, 3398, 3529
AvaI CYCGRG 3 cut(s) 711, 2069, 2150
AvaII GGWCC 2 cut(s) 1723, 2475
AxyI CCTNAGG 1 cut(s) 2361
BaeGI GKGCMC 1 cut(s) 1787
BamHI GGATCC 2 cut(s) 328, 672
BanI GGYRCC 2 cut(s) 345, 1782
BanII GRGCYC 1 cut(s) 369
BauI CACGAG 2 cut(s) 1193, 3429
BbsI GAAGAC 5 cut(s) 304, 1581, 1694, 2279, 3065
Bbv12I GWGCWC 2 cut(s) 369, 3249
BbvI GCAGC 3 cut(s) 442, 658, 862
BceAI ACGGC 2 cut(s) 337, 2249
BcgI CGANNNNNNTGC 2 cut(s) 2050, 2084
BciT130I CCWGG 4 cut(s) 448, 1545, 3117, 3507
BclI TGATCA 1 cut(s) 2443
BfaI CTAG 5 cut(s) 198, 797, 1397, 2594, 2909
BfmI CTRYAG 1 cut(s) 2269
BfrI CTTAAG 1 cut(s) 1789
BfuAI ACCTGC 3 cut(s) 761, 1940, 3190
BglII AGATCT 5 cut(s) 200, 1448, 1793, 1975, 2323
BisI GCNGC 4 cut(s) 344, 456, 647, 876
BlsI GCNGC 4 cut(s) 345, 457, 648, 877
Bme1390I CCNGG 4 cut(s) 448, 1545, 3117, 3507
Bme18I GGWCC 2 cut(s) 1723, 2475
BmeT110I CYCGRG 3 cut(s) 711, 2069, 2150
BmgT120I GGNCC 3 cut(s) 1723, 2475, 3503
BmiI GGNNCC 5 cut(s) 330, 347, 674, 1784, 2476
BmrFI CCNGG 4 cut(s) 448, 1545, 3117, 3507
BmrI ACTGGG 2 cut(s) 1788, 2360
BmsI GCATC 8 cut(s) 1036, 1473, 1804, 2330, 2531, 2944, 2966, 3020
BmuI ACTGGG 2 cut(s) 1788, 2360
BoxI GACNNNNGTC 1 cut(s) 467
BpiI GAAGAC 5 cut(s) 304, 1581, 1694, 2279, 3065
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 469
Bpu10I CCTNAGC 1 cut(s) 1173
BpuEI CTTGAG 5 cut(s) 167, 1780, 1985, 2861, 3152
BsaBI GATNNNNATC 2 cut(s) 933, 2328
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1572, 1928
BsaJI CCNNGG 4 cut(s) 585, 676, 1185, 1621
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 4 cut(s) 1424, 1627, 3256, 3644
Bse1I ACTGG 5 cut(s) 474, 1783, 2355, 3638, 3654
Bse21I CCTNAGG 1 cut(s) 2361
Bse3DI GCAATG 1 cut(s) 3040
Bse8I GATNNNNATC 2 cut(s) 933, 2328
BseBI CCWGG 4 cut(s) 448, 1545, 3117, 3507
BseDI CCNNGG 4 cut(s) 585, 676, 1185, 1621
BseJI GATNNNNATC 2 cut(s) 933, 2328
BseLI CCNNNNNNNGG 4 cut(s) 1424, 1627, 3256, 3644
BseMI GCAATG 1 cut(s) 3040
BseMII CTCAG 6 cut(s) 122, 420, 456, 2228, 2375, 2702
BseNI ACTGG 5 cut(s) 474, 1783, 2355, 3638, 3654
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 1 cut(s) 1787
BseXI GCAGC 3 cut(s) 442, 658, 862
BseYI CCCAGC 2 cut(s) 486, 1509
BsgI GTGCAG 1 cut(s) 3168
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 6 cut(s) 169, 294, 795, 1737, 2244, 3505
BshNI GGYRCC 2 cut(s) 345, 1782
BsiHKAI GWGCWC 2 cut(s) 369, 3249
BsiHKCI CYCGRG 3 cut(s) 711, 2069, 2150
BslI CCNNNNNNNGG 4 cut(s) 1424, 1627, 3256, 3644
BsmI GAATGC 3 cut(s) 612, 2653, 3044
BsnI GGCC 6 cut(s) 169, 294, 795, 1737, 2244, 3505
Bso31I GGTCTC 2 cut(s) 1572, 1928
BsoBI CYCGRG 3 cut(s) 711, 2069, 2150
Bsp1286I GDGCHC 3 cut(s) 369, 1787, 3249
Bsp19I CCATGG 1 cut(s) 676
BspACI CCGC 7 cut(s) 343, 610, 644, 685, 892, 2478, 3669
BspANI GGCC 6 cut(s) 169, 294, 795, 1737, 2244, 3505
BspCNI CTCAG 6 cut(s) 121, 421, 457, 2229, 2374, 2703
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 2 cut(s) 616, 1570
BspLI GGNNCC 5 cut(s) 330, 347, 674, 1784, 2476
BspMAI CTGCAG 1 cut(s) 2273
BspMI ACCTGC 3 cut(s) 761, 1940, 3190
BspPI GGATC 7 cut(s) 323, 336, 667, 680, 942, 1152, 1386
BspT107I GGYRCC 2 cut(s) 345, 1782
BspTI CTTAAG 1 cut(s) 1789
BspTNI GGTCTC 2 cut(s) 1572, 1928
BsrDI GCAATG 1 cut(s) 3040
BsrI ACTGG 5 cut(s) 474, 1783, 2355, 3638, 3654
BssECI CCNNGG 4 cut(s) 585, 676, 1185, 1621
BssNAI GTATAC 2 cut(s) 567, 2906
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1193, 3429
BssT1I CCWWGG 4 cut(s) 585, 676, 1185, 1621
Bst1107I GTATAC 2 cut(s) 567, 2906
Bst2BI CACGAG 2 cut(s) 1193, 3429
Bst2UI CCWGG 4 cut(s) 448, 1545, 3117, 3507
Bst4CI ACNGT 8 cut(s) 780, 1076, 1479, 1530, 2129, 2387, 3200, 3467
Bst6I CTCTTC 2 cut(s) 19, 3328
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1789
BstAPI GCANNNNNTGC 3 cut(s) 875, 1492, 3556
BstC8I GCNNGC 5 cut(s) 683, 1354, 1409, 3363, 3680
BstDSI CCRYGG 1 cut(s) 676
BstEII GGTNACC 1 cut(s) 2773
BstFNI CGCG 1 cut(s) 70
BstHHI GCGC 1 cut(s) 72
BstMWI GCNNNNNNNGC 9 cut(s) 452, 687, 875, 1492, 2836, 3224, 3556, 3666, 3675
BstNI CCWGG 4 cut(s) 448, 1545, 3117, 3507
BstNSI RCATGY 1 cut(s) 685
BstPAI GACNNNNGTC 1 cut(s) 467
BstPI GGTNACC 1 cut(s) 2773
BstSCI CCNGG 4 cut(s) 446, 1543, 3115, 3505
BstSFI CTRYAG 1 cut(s) 2269
BstSLI GKGCMC 1 cut(s) 1787
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 3 cut(s) 442, 658, 862
BstV2I GAAGAC 5 cut(s) 304, 1581, 1694, 2279, 3065
BstX2I RGATCY 8 cut(s) 200, 328, 672, 1144, 1448, 1793, 1975, 2323
BstYI RGATCY 8 cut(s) 200, 328, 672, 1144, 1448, 1793, 1975, 2323
BstZ17I GTATAC 2 cut(s) 567, 2906
Bsu36I CCTNAGG 1 cut(s) 2361
BsuRI GGCC 6 cut(s) 169, 294, 795, 1737, 2244, 3505
BtgI CCRYGG 1 cut(s) 676
BtsI GCAGTG 2 cut(s) 3598, 3667
BtsIMutI CAGTG 5 cut(s) 1475, 2936, 3598, 3661, 3667
BveI ACCTGC 3 cut(s) 761, 1940, 3190
Cac8I GCNNGC 5 cut(s) 683, 1354, 1409, 3363, 3680
CaiI CAGNNNCTG 1 cut(s) 875
CciI TCATGA 2 cut(s) 616, 1570
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 3 cut(s) 1723, 2475, 3503
Csp6I GTAC 3 cut(s) 2931, 3236, 3447
CspCI CAANNNNNGTGG 2 cut(s) 1911, 1946
CviQI GTAC 3 cut(s) 2931, 3236, 3447
Eam1104I CTCTTC 2 cut(s) 19, 3328
EarI CTCTTC 2 cut(s) 19, 3328
EciI GGCGGA 1 cut(s) 3684
Ecl136II GAGCTC 1 cut(s) 367
Eco130I CCWWGG 4 cut(s) 585, 676, 1185, 1621
Eco147I AGGCCT 2 cut(s) 169, 1737
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 2 cut(s) 1572, 1928
Eco47I GGWCC 2 cut(s) 1723, 2475
Eco53kI GAGCTC 1 cut(s) 367
Eco57I CTGAAG 5 cut(s) 1613, 2799, 3077, 3341, 3533
Eco81I CCTNAGG 1 cut(s) 2361
Eco88I CYCGRG 3 cut(s) 711, 2069, 2150
Eco91I GGTNACC 1 cut(s) 2773
EcoICRI GAGCTC 1 cut(s) 367
EcoO65I GGTNACC 1 cut(s) 2773
EcoRI GAATTC 2 cut(s) 117, 3278
EcoRII CCWGG 4 cut(s) 446, 1543, 3115, 3505
EcoT14I CCWWGG 4 cut(s) 585, 676, 1185, 1621
EcoT22I ATGCAT 3 cut(s) 281, 1488, 2959
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 4 cut(s) 585, 676, 1185, 1621
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FauI CCCGC 2 cut(s) 617, 678
FauNDI CATATG 1 cut(s) 638
FbaI TGATCA 1 cut(s) 2443
FblI GTMKAC 4 cut(s) 566, 2417, 2495, 2905
Fnu4HI GCNGC 4 cut(s) 344, 456, 647, 876
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 4 cut(s) 344, 456, 647, 876
FspBI CTAG 5 cut(s) 198, 797, 1397, 2594, 2909
GlaI GCGC 1 cut(s) 71
GluI GCNGC 4 cut(s) 344, 456, 647, 876
GsaI CCCAGC 2 cut(s) 490, 1513
GsuI CTGGAG 1 cut(s) 469
HaeIII GGCC 6 cut(s) 169, 294, 795, 1737, 2244, 3505
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 3 cut(s) 9, 942, 1641
HindII GTYRAC 3 cut(s) 9, 942, 1641
HindIII AAGCTT 2 cut(s) 2837, 3323
HphI GGTGA 8 cut(s) 115, 1088, 1732, 2389, 2741, 2785, 3398, 3529
Hpy166II GTNNAC 9 cut(s) 9, 567, 942, 1526, 1641, 1723, 2418, 2496, 2906
Hpy8I GTNNAC 9 cut(s) 9, 567, 942, 1526, 1641, 1723, 2418, 2496, 2906
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 8 cut(s) 780, 1076, 1479, 1530, 2129, 2387, 3200, 3467
HpyCH4IV ACGT 3 cut(s) 1552, 1719, 2643
HpyF10VI GCNNNNNNNGC 9 cut(s) 452, 687, 875, 1492, 2836, 3224, 3556, 3666, 3675
HpySE526I ACGT 3 cut(s) 1552, 1719, 2643
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 2443
LmnI GCTCC 2 cut(s) 872, 2735
Lsp1109I GCAGC 3 cut(s) 442, 658, 862
LweI GCATC 8 cut(s) 1036, 1473, 1804, 2330, 2531, 2944, 2966, 3020
MaeI CTAG 5 cut(s) 198, 797, 1397, 2594, 2909
MaeII ACGT 3 cut(s) 1552, 1719, 2643
MaeIII GTNAC 5 cut(s) 968, 1384, 1715, 2756, 2773
MfeI CAATTG 3 cut(s) 186, 2226, 3001
MflI RGATCY 8 cut(s) 200, 328, 672, 1144, 1448, 1793, 1975, 2323
MhlI GDGCHC 3 cut(s) 369, 1787, 3249
MlyI GAGTC 4 cut(s) 203, 2083, 2108, 3540
MmeI TCCRAC 3 cut(s) 1296, 1491, 2140
Mph1103I ATGCAT 3 cut(s) 281, 1488, 2959
MroXI GAANNNNTTC 7 cut(s) 604, 816, 1086, 1518, 1612, 3062, 3099
MslI CAYNNNNRTG 4 cut(s) 128, 244, 599, 3150
MspCI CTTAAG 1 cut(s) 1789
MspR9I CCNGG 4 cut(s) 448, 1545, 3117, 3507
MunI CAATTG 3 cut(s) 186, 2226, 3001
Mva1269I GAATGC 3 cut(s) 612, 2653, 3044
MvaI CCWGG 4 cut(s) 448, 1545, 3117, 3507
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 9 cut(s) 452, 687, 875, 1492, 2836, 3224, 3556, 3666, 3675
NcoI CCATGG 1 cut(s) 676
NdeI CATATG 1 cut(s) 638
NlaIV GGNNCC 5 cut(s) 330, 347, 674, 1784, 2476
NmuCI GTSAC 3 cut(s) 968, 1384, 2773
NsiI ATGCAT 3 cut(s) 281, 1488, 2959
NspI RCATGY 1 cut(s) 685
PaeI GCATGC 1 cut(s) 685
PaeR7I CTCGAG 1 cut(s) 2069
PagI TCATGA 2 cut(s) 616, 1570
PaqCI CACCTGC 1 cut(s) 1940
PceI AGGCCT 2 cut(s) 169, 1737
PctI GAATGC 3 cut(s) 612, 2653, 3044
PdmI GAANNNNTTC 7 cut(s) 604, 816, 1086, 1518, 1612, 3062, 3099
PflMI CCANNNNNTGG 2 cut(s) 1627, 3256
PfoI TCCNGGA 1 cut(s) 3115
PkrI GCNGC 4 cut(s) 345, 457, 648, 877
PleI GAGTC 4 cut(s) 202, 2082, 2107, 3539
PpsI GAGTC 4 cut(s) 202, 2082, 2107, 3539
PshAI GACNNNNGTC 1 cut(s) 467
PshBI ATTAAT 1 cut(s) 2114
PsiI TTATAA 1 cut(s) 3694
Psp124BI GAGCTC 1 cut(s) 369
Psp6I CCWGG 4 cut(s) 446, 1543, 3115, 3505
PspEI GGTNACC 1 cut(s) 2773
PspFI CCCAGC 2 cut(s) 486, 1509
PspGI CCWGG 4 cut(s) 446, 1543, 3115, 3505
PspN4I GGNNCC 5 cut(s) 330, 347, 674, 1784, 2476
PspPI GGNCC 3 cut(s) 1723, 2475, 3503
PstI CTGCAG 1 cut(s) 2273
PstNI CAGNNNCTG 1 cut(s) 875
PsuI RGATCY 8 cut(s) 200, 328, 672, 1144, 1448, 1793, 1975, 2323
RsaI GTAC 3 cut(s) 2932, 3237, 3448
RsaNI GTAC 3 cut(s) 2931, 3236, 3447
RseI CAYNNNNRTG 4 cut(s) 128, 244, 599, 3150
SacI GAGCTC 1 cut(s) 369
SatI GCNGC 4 cut(s) 344, 456, 647, 876
Sau96I GGNCC 3 cut(s) 1723, 2475, 3503
SchI GAGTC 4 cut(s) 203, 2083, 2108, 3540
ScrFI CCNGG 4 cut(s) 448, 1545, 3117, 3507
SduI GDGCHC 3 cut(s) 369, 1787, 3249
SfaNI GCATC 8 cut(s) 1036, 1473, 1804, 2330, 2531, 2944, 2966, 3020
SfcI CTRYAG 1 cut(s) 2269
Sfr274I CTCGAG 1 cut(s) 2069
SinI GGWCC 2 cut(s) 1723, 2475
SlaI CTCGAG 1 cut(s) 2069
SmiMI CAYNNNNRTG 4 cut(s) 128, 244, 599, 3150
SmlI CTYRAG 7 cut(s) 146, 1759, 1789, 1964, 2069, 2840, 3131
SmoI CTYRAG 7 cut(s) 146, 1759, 1789, 1964, 2069, 2840, 3131
SphI GCATGC 1 cut(s) 685
SseBI AGGCCT 2 cut(s) 169, 1737
SsiI CCGC 7 cut(s) 343, 610, 644, 685, 892, 2478, 3669
SspI AATATT 2 cut(s) 1338, 3127
SspMI CTAG 5 cut(s) 198, 797, 1397, 2594, 2909
SstI GAGCTC 1 cut(s) 369
StuI AGGCCT 2 cut(s) 169, 1737
StyD4I CCNGG 4 cut(s) 446, 1543, 3115, 3505
StyI CCWWGG 4 cut(s) 585, 676, 1185, 1621
TaaI ACNGT 8 cut(s) 780, 1076, 1479, 1530, 2129, 2387, 3200, 3467
TaiI ACGT 3 cut(s) 1555, 1722, 2646
TaqI TCGA 2 cut(s) 985, 2070
TaqII GACCGA 2 cut(s) 153, 762
TatI WGTACW 2 cut(s) 2930, 3235
TauI GCSGC 1 cut(s) 346
TscAI CASTG 5 cut(s) 1482, 2943, 3598, 3661, 3667
TseFI GTSAC 3 cut(s) 968, 1384, 2773
TseI GCWGC 3 cut(s) 455, 646, 875
Tsp45I GTSAC 3 cut(s) 968, 1384, 2773
TspGWI ACGGA 2 cut(s) 238, 3524
TspRI CASTG 5 cut(s) 1482, 2943, 3598, 3661, 3667
Van91I CCANNNNNTGG 2 cut(s) 1627, 3256
Vha464I CTTAAG 1 cut(s) 1789
VpaK11BI GGWCC 2 cut(s) 1723, 2475
VspI ATTAAT 1 cut(s) 2114
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 685
XcmI CCANNNNNNNNNTGG 1 cut(s) 832
XhoI CTCGAG 1 cut(s) 2069
XmiI GTMKAC 4 cut(s) 566, 2417, 2495, 2905
XmnI GAANNNNTTC 7 cut(s) 604, 816, 1086, 1518, 1612, 3062, 3099
XspI CTAG 5 cut(s) 198, 797, 1397, 2594, 2909
Zsp2I ATGCAT 3 cut(s) 281, 1488, 2959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.