Rw6G000680

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000105
Physical Location & Seq
Forward (+)
1136605 .. 1141010
4406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000680.1

Sequence Viewer

Length: 3627 bp
ATGTCGTCAACACCCTCTTCTTCTTCTACTCCTCCTCGTCAATGGAAGTATGATGTTTTTCTTAGTTTCAGAGGAGAAGACACCCGCTTGAATTTTGTGGACCATCTGTATGCTGCATTAGGTCAGAAAGGAATTGTCACCTTTAGAGATGACAAAAGTCTTGAGAGAGGAAAATCCATTTCGTCAGAACTTCTTAAAGCAATTGAGGAGTCAAAATTTGCTCTTGTCATTCTTTCACCAAACTATGCTTCTTCTTCATGGTGCTTAGACGAGCTTTTGAAGATTATTCAATGCAGAGAAGAAATGGGGCAACAAGTCATGCCAGTGTTCTACCAAGTAGATCCATCTCATGTGCGTAAGAACAAGGGATATTTTTTGCAGGCCTTTACAGAACACAAAGAAGTTCATTGGGAGAACAGGGATAAAGTAAGGAAGTGGAGGGCTGCTTTGACAGGCGTGGCCAATCTTTCTGGGTGGGATTTGAGGAACATGCATGAGGCAAAAGCTATCAAGAGTATTGTTAAAGATATACTAAATAAATCACAGTATTCATCTACAAGCGTTTACAAGGGCATGGTAGGAATGGATGTCCGTGTTGATGATTTACTGAGAAATCATATTCGTCCACATTTTGATGACGTGCGCATGATAGGGATCCATGGAATGCGGGGAATAGGTAAGACGGCAATTGCCCGAGCTCTTTATGATCAACTTGGTCAAGATTTTGACAGTAGCTGCGAGCCTTCCAATGTTAGAGAAGTGTTTAGCAAAGGTGGTATATATCCTTCACAGTATGAAATTAGTGACTGTTTTGACCATTGCTGCTTTCTTTCCAACGTTAGAGAAATGTCAAAAAAAGATGGTCTAGTTTCTCTTCAAGAAAAACTTCTTTCCAGAGTCTTGAGGGCAACAATTAGGAACATAGAGGATGAATATGCTGGAGCGAATATGATACAAAGACGATTAGGTAAATTAAGGGTGCTGATTGTTATTGATGATGTGGATCAAGTGGCACAATTAGAATGTTTGGCTGGAAATCATGACTGGTTCGGTCCGGGAAGTAGAATTATCATAACCAGTGCTGATGTTCATTTGTTGAATTGTGTGGATACTCTATACAAGGCTGCTGTGTTAACCCATGATGAAGCTCTTCAACTTTTGAGTTTAAAGGCTTTTAAGAAAAACCCCCCGCCTGAAGATTACTTGGAGCTATGCAACAATATTTTAGGGTATGCTCATGGGCTTCCATTAGTTCTTGTGGTTATAGGTCCATTTCTATGTGGTAGATCGATCAATGAATGGAAGAGTGCAATCGATAGGCTCAGGAATAAACCAGAAACTAGCATCGTTGATGTGCTTCAGATAAGTTTTGATGGGCTAAGGGAAACAGAAAAAGAAATTTTCCTACATGTCGCTTGCTTTTATGTGGGGAAGGATAGGGATCGTGTATCAGAAATACTAGATTATTGTCAGCTTTACCCTGACATTGGTTTAAGTGTTCTTGCTGATAAATCTCTCATTACTATTTCCAACAACAAACTATCCATGCATGCTTTGCTACAAGAAATGGGCTGGGAGATAGTTCGTAGGGAGTCACCTAAACCAGGCAGGCGTACTAGATTATGGTCTCATGAAGACATCCACAATGTGCTGAAAAAAAATAAGGGAACAGAAGCAGTTCAAGGCATGGTAATGGACTTGCCTAAATTAGAAGTGGCTAATTGGAACCCCGAAGCATTCTCAAAGTTGTCTGAGCTTAACTTTCTCCAAATCCGTAATGTCGACCTTCCCAATGGCCTCACTTGCCTCTCTAATTCCTTGAGACTCCTCGAATGGAGTGAGTATCCCTTAAGATCTCTGCCACAAAATTTCGAAGCAGATGAACTTATTGAACTTAATTTGTGCCACAGCAAAATTGAACATCTTTGGGAGGGAACAAAGTGTTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAGAACATTGTCCAGACCCCAGACCTCACAGGCGCTTGTAGTCTTGAGTGTCTAGATCTTGAAGGGTGTAAAAACTTGGTAAAAATCCATCAATCCGTTGGAGTTCTCAAAAAGCTTATTGTCTTGAATCTTAAAGACTGTGAGAGTCTGCAGAGTCTACCAGATAGAATTGAAATGGAGTCTCTTAAAACTCTTATTCTTTCTGGGTGTTCCAATATCAAGAAGATTCCTGAGTTTAGCAGAAATATGGAGTGTTTGTCAGAGCTGTGTCTTGATAGGACTGCCATTGAAAAACTGCCCGACTCAATCCAGTACCTATGTGGCCTGTCTTTCTTGGATCTAAGCAACTGCAAGAATCTTTTTTGTCTCCCAAGCACCATTAATAGATTGACAAGTCTTAAAAATATTAATCTTTCAGGATGCCAGAAGCTCGGGGGACCGGATAAAAGTGTGCGGGAGTTGGATTTTTTGCCGGCAAATGTTGCAAGTATAACTCCTGTAACAGAATTACCATCATCGGTCTTCAGCATTTCAGATCATGTTAAAGATTCAAGTTCTCATGGATATGAAGTCATTCAAGAATCATTGTTCGAGTTTTTTCCTTCTGGATTGATGCAAATGGCGTATGAAGAGCCAACGAGTTTCCGGTTGTCTTTATCTGGTCTGGGTAATTTAACAGATCTGAACATAAGTGACTGCAATCTTGATGACAGATCATTTGTCAACAACTTTGGCTGGTTGCCCTGTTTAGTGGCATTAAATCTGAGTGGAAATGATTTTATTAAGCTTCCTCCAACCATCAGATGGCTTAATAAGCTTGAGAACGTTAACTTGGAGAACTGCAAAAGACTTGAAGACTTGTCAAATCTTCCATCAAATAGTAAGTTGGATGTAAGAGCTGATGGTTGCTCTTCATTGAGAATTTTGTTTGACATATCCAATTACAACAGACTGGAGGAATCATATTTCAGTTTCATCAACTGCTTTAAGCTAAATGAAGACCGAGGCCGCAGTAATATAGCATTTGAAATGCTGAAGATAGTCCTTCATCAGGCTATCTCCACCACAAAGGAAACATTTCAAATTGTATTTCCTGGAAGTGAAATTCCTCGGTGGTTCAAATATCAAAGCCGTAAGGATTCATTGGGTGTATGCATACTACCTCCACGTCCATATTCCAGTAGGTTTATGGGATTTGCCTTGTGTGCTGTTTTTGTAATCAATGATGAGCGCCATCAGGTTGATGAACTTGAGATCCATCACTTCAAGACTTTTAAAGCTACGCATAATCTAGTCTGTTACCTGAAACTCAATGGAAAAGAATTAGAAGTATGTGGCAGACAGCCGGCCTTTCGGTTTAGTGAAGAATTTTGCCAGGTTGAGTCTGATCACCGTTGGGTATTCTATGTATCCTCGGATAAATACTTTGGTATTGAAGAGCATGACAGATGCTCTCAGATTGAACTGTTATTTGAAGCCAGAGGGCCAGGTTTGAAGGTGAAGGCGTGCGGGCTCCGTTTGATATTTGAACAAGATGTGCACGAGTTGATGAAGGGATGTAGACACAAACAAGCATACGGACGGCACGGATACGTACCGAAATATGAATGTGATTGTTGGGTGGATGGCTATTTATATTCTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1208

Amino Acids

137.68

Weight (kDa)

6.25

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 193 5.5e-58 TIR domain
TIR_2 PF13676 19 - 116 3.1e-17 TIR domain
NB-ARC PF00931 263 - 395 1.3e-08 NB-ARC domain
WHD_ROQ1 PF23282 462 - 532 5.5e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 629 - 647 4.2e-06 Leucine Rich Repeat
LRR_13 PF23286 720 - 805 3e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1024 - 1173 1.4e-15 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 644
AccB7I CCANNNNNTGG 1 cut(s) 2784
AccI GTMKAC 3 cut(s) 1782, 2137, 3541
AciI CCGC 6 cut(s) 85, 667, 1190, 2434, 2989, 3489
AclI AACGTT 2 cut(s) 837, 2805
AclWI GGATC 7 cut(s) 335, 649, 662, 1011, 1449, 2325, 3229
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 7 cut(s) 91, 215, 1398, 1867, 2901, 3084, 3347
AcuI CTGAAG 4 cut(s) 1215, 1343, 2488, 3035
AdeI CACNNNGTG 1 cut(s) 1648
AfaI GTAC 3 cut(s) 1615, 2294, 3576
AfiI CCNNNNNNNGG 4 cut(s) 1487, 1604, 2784, 3031
AflII CTTAAG 1 cut(s) 1849
AflIII ACRYGT 1 cut(s) 1408
AjiI CACGTC 2 cut(s) 640, 3149
AjnI CCWGG 4 cut(s) 1603, 3073, 3354, 3466
AjuI GAANNNNNNNTTGG 4 cut(s) 232, 264, 2795, 2827
AloI GAACNNNNNNTCC 2 cut(s) 3219, 3251
Alw21I GWGCWC 2 cut(s) 700, 3522
Alw26I GTCTC 4 cut(s) 1632, 1816, 2166, 2351
Alw44I GTGCAC 1 cut(s) 3518
AlwI GGATC 7 cut(s) 335, 649, 662, 1011, 1449, 2325, 3229
AlwNI CAGNNNCTG 1 cut(s) 735
Ama87I CYCGRG 2 cut(s) 693, 2411
AoxI GGCC 7 cut(s) 381, 459, 1795, 2302, 2986, 3327, 3464
ApaLI GTGCAC 1 cut(s) 3518
ApeKI GCWGC 5 cut(s) 113, 443, 735, 822, 1124
ApoI RAATTY 7 cut(s) 91, 215, 1398, 1867, 2901, 3084, 3347
ArsI GACNNNNNNTTYG 2 cut(s) 2681, 2713
AseI ATTAAT 2 cut(s) 2361, 2388
Asp700I GAANNNNTTC 5 cut(s) 885, 1149, 1677, 2553, 3057
AspLEI GCGC 3 cut(s) 645, 2015, 3213
AspS9I GGNCC 5 cut(s) 100, 1052, 1268, 2417, 3464
AsuC2I CCSGG 1 cut(s) 1056
AsuHPI GGTGA 5 cut(s) 130, 228, 1587, 3362, 3490
AsuII TTCGAA 1 cut(s) 1872
AvaI CYCGRG 2 cut(s) 693, 2411
AvaII GGWCC 4 cut(s) 100, 1052, 1268, 2417
BaeGI GKGCMC 1 cut(s) 3522
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 1 cut(s) 654
BanII GRGCYC 2 cut(s) 700, 3495
BarI GAAGNNNNNNTAC 2 cut(s) 769, 801
BauI CACGAG 1 cut(s) 3521
BbsI GAAGAC 5 cut(s) 84, 1641, 2494, 2841, 2985
Bbv12I GWGCWC 2 cut(s) 700, 3522
BbvI GCAGC 5 cut(s) 100, 430, 722, 809, 1111
BceAI ACGGC 3 cut(s) 699, 3096, 3578
BciT130I CCWGG 4 cut(s) 1605, 3075, 3356, 3468
BciVI GTATCC 4 cut(s) 1102, 1854, 3400, 3563
BclI TGATCA 2 cut(s) 706, 3367
BcnI CCSGG 1 cut(s) 1056
BcoDI GTCTC 4 cut(s) 1632, 1816, 2166, 2351
BfaI CTAG 6 cut(s) 866, 1341, 1460, 1617, 2033, 3272
BfmI CTRYAG 1 cut(s) 2129
BfoI RGCGCY 2 cut(s) 2016, 3214
BfrI CTTAAG 1 cut(s) 1849
BfuI GTATCC 4 cut(s) 1102, 1854, 3400, 3563
BglII AGATCT 3 cut(s) 1853, 2035, 2659
BisI GCNGC 6 cut(s) 114, 444, 736, 823, 1125, 2989
BlsI GCNGC 6 cut(s) 115, 445, 737, 824, 1126, 2990
Bme1390I CCNGG 5 cut(s) 1056, 1605, 3075, 3356, 3468
Bme18I GGWCC 4 cut(s) 100, 1052, 1268, 2417
BmeT110I CYCGRG 2 cut(s) 693, 2411
BmgBI CACGTC 2 cut(s) 640, 3149
BmgT120I GGNCC 5 cut(s) 100, 1052, 1268, 2417, 3464
BmiI GGNNCC 4 cut(s) 656, 1727, 2418, 3494
BmrFI CCNGG 5 cut(s) 1056, 1605, 3075, 3356, 3468
BmsI GCATC 4 cut(s) 1353, 2390, 2583, 3419
BoxI GACNNNNGTC 1 cut(s) 156
BpiI GAAGAC 5 cut(s) 84, 1641, 2494, 2841, 2985
BpmI CTGGAG 2 cut(s) 960, 2954
Bpu10I CCTNAGC 2 cut(s) 1322, 1379
Bpu14I TTCGAA 1 cut(s) 1872
BpuEI CTTGAG 6 cut(s) 182, 922, 1840, 2045, 2819, 3251
BpuMI CCSGG 1 cut(s) 1056
Bsa29I ATCGAT 2 cut(s) 1289, 1314
BsaAI YACGTR 1 cut(s) 3574
BsaBI GATNNNNATC 3 cut(s) 653, 1002, 1440
BsaI GGTCTC 1 cut(s) 1632
BsaJI CCNNGG 4 cut(s) 658, 2983, 3089, 3393
BsaWI WCCGGW 2 cut(s) 2419, 2625
BsaXI ACNNNNNCTCC 2 cut(s) 2222, 2252
Bsc4I CCNNNNNNNGG 4 cut(s) 1487, 1604, 2784, 3031
Bse118I RCCGGY 2 cut(s) 2452, 3325
Bse1I ACTGG 6 cut(s) 323, 1049, 1077, 2290, 2937, 3159
Bse3DI GCAATG 1 cut(s) 817
Bse8I GATNNNNATC 3 cut(s) 653, 1002, 1440
BseBI CCWGG 4 cut(s) 1605, 3075, 3356, 3468
BseCI ATCGAT 2 cut(s) 1289, 1314
BseDI CCNNGG 4 cut(s) 658, 2983, 3089, 3393
BseGI GGATG 7 cut(s) 592, 934, 1638, 2405, 2875, 3542, 3610
BseJI GATNNNNATC 3 cut(s) 653, 1002, 1440
BseLI CCNNNNNNNGG 4 cut(s) 1487, 1604, 2784, 3031
BseMI GCAATG 1 cut(s) 817
BseMII CTCAG 7 cut(s) 599, 1336, 1743, 1994, 2202, 2735, 3449
BseNI ACTGG 6 cut(s) 323, 1049, 1077, 2290, 2937, 3159
BseRI GAGGAG 5 cut(s) 21, 24, 87, 221, 1817
BseSI GKGCMC 1 cut(s) 3522
BseXI GCAGC 5 cut(s) 100, 430, 722, 809, 1111
BseYI CCCAGC 1 cut(s) 1572
BshFI GGCC 7 cut(s) 383, 461, 1797, 2304, 2988, 3329, 3466
BshVI ATCGAT 2 cut(s) 1289, 1314
BsiHKAI GWGCWC 2 cut(s) 700, 3522
BsiHKCI CYCGRG 2 cut(s) 693, 2411
BsiSI CCGG 5 cut(s) 1055, 2420, 2453, 2626, 3326
BslFI GGGAC 1 cut(s) 2430
BslI CCNNNNNNNGG 4 cut(s) 1487, 1604, 2784, 3031
BsmAI GTCTC 4 cut(s) 1632, 1816, 2166, 2351
BsmFI GGGAC 1 cut(s) 2430
BsmI GAATGC 2 cut(s) 669, 1736
BsnI GGCC 7 cut(s) 383, 461, 1797, 2304, 2988, 3329, 3466
Bso31I GGTCTC 1 cut(s) 1632
BsoBI CYCGRG 2 cut(s) 693, 2411
Bsp119I TTCGAA 1 cut(s) 1872
Bsp1286I GDGCHC 3 cut(s) 700, 3495, 3522
Bsp19I CCATGG 1 cut(s) 658
BspACI CCGC 6 cut(s) 85, 667, 1190, 2434, 2989, 3489
BspANI GGCC 7 cut(s) 383, 461, 1797, 2304, 2988, 3329, 3466
BspCNI CTCAG 7 cut(s) 600, 1335, 1744, 1993, 2203, 2736, 3448
BspDI ATCGAT 2 cut(s) 1289, 1314
BspHI TCATGA 2 cut(s) 1039, 1630
BspLI GGNNCC 4 cut(s) 656, 1727, 2418, 3494
BspMAI CTGCAG 1 cut(s) 2133
BspPI GGATC 7 cut(s) 335, 649, 662, 1011, 1449, 2325, 3229
BspQI GCTCTTC 4 cut(s) 1155, 2604, 2896, 3411
BspT104I TTCGAA 1 cut(s) 1872
BspTI CTTAAG 1 cut(s) 1849
BspTNI GGTCTC 1 cut(s) 1632
BsrDI GCAATG 1 cut(s) 817
BsrFI RCCGGY 2 cut(s) 2452, 3325
BsrI ACTGG 6 cut(s) 323, 1049, 1077, 2290, 2937, 3159
BssAI RCCGGY 2 cut(s) 2452, 3325
BssECI CCNNGG 4 cut(s) 658, 2983, 3089, 3393
BssSI CACGAG 1 cut(s) 3521
BssT1I CCWWGG 1 cut(s) 658
Bst2BI CACGAG 1 cut(s) 3521
Bst2UI CCWGG 4 cut(s) 1605, 3075, 3356, 3468
Bst4CI ACNGT 7 cut(s) 546, 731, 792, 809, 2120, 3374, 3447
Bst6I CTCTTC 7 cut(s) 22, 879, 1155, 1298, 2604, 2896, 3411
BstAFI CTTAAG 1 cut(s) 1849
BstAPI GCANNNNNTGC 2 cut(s) 1555, 2462
BstBAI YACGTR 1 cut(s) 3574
BstBI TTCGAA 1 cut(s) 1872
BstC8I GCNNGC 9 cut(s) 381, 740, 1417, 1551, 1610, 2454, 3327, 3487, 3491
BstDSI CCRYGG 1 cut(s) 658
BstENI CCTNNNNNAGG 2 cut(s) 1602, 3029
BstF5I GGATG 7 cut(s) 592, 934, 1638, 2405, 2875, 3542, 3610
BstH2I RGCGCY 2 cut(s) 2016, 3214
BstHHI GCGC 3 cut(s) 645, 2015, 3213
BstMAI GTCTC 4 cut(s) 1632, 1816, 2166, 2351
BstMWI GCNNNNNNNGC 5 cut(s) 1555, 1803, 2462, 2794, 3185
BstNI CCWGG 4 cut(s) 1605, 3075, 3356, 3468
BstNSI RCATGY 3 cut(s) 493, 1412, 1553
BstPAI GACNNNNGTC 1 cut(s) 156
BstSCI CCNGG 5 cut(s) 1054, 1603, 3073, 3354, 3466
BstSFI CTRYAG 1 cut(s) 2129
BstSLI GKGCMC 1 cut(s) 3522
BstSNI TACGTA 1 cut(s) 3574
BstV1I GCAGC 5 cut(s) 100, 430, 722, 809, 1111
BstV2I GAAGAC 5 cut(s) 84, 1641, 2494, 2841, 2985
BstX2I RGATCY 7 cut(s) 340, 654, 1853, 2035, 2317, 2659, 3234
BstYI RGATCY 7 cut(s) 340, 654, 1853, 2035, 2317, 2659, 3234
Bsu15I ATCGAT 2 cut(s) 1289, 1314
BsuI GTATCC 4 cut(s) 1102, 1854, 3400, 3563
BsuRI GGCC 7 cut(s) 383, 461, 1797, 2304, 2988, 3329, 3466
BsuTUI ATCGAT 2 cut(s) 1289, 1314
BtgI CCRYGG 1 cut(s) 658
BtrI CACGTC 2 cut(s) 640, 3149
BtsCI GGATG 7 cut(s) 592, 934, 1638, 2405, 2875, 3542, 3610
BtsIMutI CAGTG 2 cut(s) 330, 1084
Cac8I GCNNGC 9 cut(s) 381, 740, 1417, 1551, 1610, 2454, 3327, 3487, 3491
CaiI CAGNNNCTG 1 cut(s) 735
CciI TCATGA 2 cut(s) 1039, 1630
CfoI GCGC 3 cut(s) 645, 2015, 3213
Cfr10I RCCGGY 2 cut(s) 2452, 3325
Cfr13I GGNCC 5 cut(s) 100, 1052, 1268, 2417, 3464
ClaI ATCGAT 2 cut(s) 1289, 1314
CpoI CGGWCCG 1 cut(s) 1052
Csp6I GTAC 3 cut(s) 1614, 2293, 3575
CspI CGGWCCG 1 cut(s) 1052
CviQI GTAC 3 cut(s) 1614, 2293, 3575
DraI TTTAAA 2 cut(s) 1167, 3256
DraIII CACNNNGTG 1 cut(s) 1648
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 7 cut(s) 22, 879, 1155, 1298, 2604, 2896, 3411
EarI CTCTTC 7 cut(s) 22, 879, 1155, 1298, 2604, 2896, 3411
Ecl136II GAGCTC 1 cut(s) 698
Eco105I TACGTA 1 cut(s) 3574
Eco130I CCWWGG 1 cut(s) 658
Eco147I AGGCCT 1 cut(s) 383
Eco24I GRGCYC 2 cut(s) 700, 3495
Eco31I GGTCTC 1 cut(s) 1632
Eco47I GGWCC 4 cut(s) 100, 1052, 1268, 2417
Eco53kI GAGCTC 1 cut(s) 698
Eco57I CTGAAG 4 cut(s) 1215, 1343, 2488, 3035
Eco88I CYCGRG 2 cut(s) 693, 2411
EcoICRI GAGCTC 1 cut(s) 698
EcoNI CCTNNNNNAGG 2 cut(s) 1602, 3029
EcoRII CCWGG 4 cut(s) 1603, 3073, 3354, 3466
EcoT14I CCWWGG 1 cut(s) 658
EcoT22I ATGCAT 3 cut(s) 495, 1551, 3137
EcoT38I GRGCYC 2 cut(s) 700, 3495
ErhI CCWWGG 1 cut(s) 658
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FaqI GGGAC 1 cut(s) 2430
FauI CCCGC 5 cut(s) 92, 660, 1197, 2427, 3482
FbaI TGATCA 2 cut(s) 706, 3367
FblI GTMKAC 3 cut(s) 1782, 2137, 3541
Fnu4HI GCNGC 6 cut(s) 114, 444, 736, 823, 1125, 2989
FokI GGATG 7 cut(s) 599, 941, 1625, 2412, 2882, 3549, 3617
FriOI GRGCYC 2 cut(s) 700, 3495
Fsp4HI GCNGC 6 cut(s) 114, 444, 736, 823, 1125, 2989
FspAI RTGCGCAY 1 cut(s) 644
FspBI CTAG 6 cut(s) 866, 1341, 1460, 1617, 2033, 3272
FspI TGCGCA 1 cut(s) 644
GlaI GCGC 3 cut(s) 644, 2014, 3212
GluI GCNGC 6 cut(s) 114, 444, 736, 823, 1125, 2989
GsaI CCCAGC 1 cut(s) 1576
GsuI CTGGAG 2 cut(s) 960, 2954
HaeII RGCGCY 2 cut(s) 2016, 3214
HaeIII GGCC 7 cut(s) 383, 461, 1797, 2304, 2988, 3329, 3466
HapII CCGG 5 cut(s) 1055, 2420, 2453, 2626, 3326
HhaI GCGC 3 cut(s) 645, 2015, 3213
Hin6I GCGC 3 cut(s) 643, 2013, 3211
HinP1I GCGC 3 cut(s) 643, 2013, 3211
HincII GTYRAC 5 cut(s) 9, 1134, 1783, 2704, 2809
HindII GTYRAC 5 cut(s) 9, 1134, 1783, 2704, 2809
HindIII AAGCTT 3 cut(s) 2093, 2765, 2795
HpaI GTTAAC 2 cut(s) 1134, 2809
HpaII CCGG 5 cut(s) 1055, 2420, 2453, 2626, 3326
HphI GGTGA 5 cut(s) 130, 228, 1587, 3362, 3490
HpyCH4III ACNGT 7 cut(s) 546, 731, 792, 809, 2120, 3374, 3447
HpyCH4IV ACGT 5 cut(s) 639, 837, 2805, 3148, 3573
HpyF10VI GCNNNNNNNGC 5 cut(s) 1555, 1803, 2462, 2794, 3185
HpySE526I ACGT 5 cut(s) 639, 837, 2805, 3148, 3573
HspAI GCGC 3 cut(s) 643, 2013, 3211
KroI GCCGGC 2 cut(s) 2452, 3325
KroNI GCCGGC 2 cut(s) 2454, 3327
Ksp22I TGATCA 2 cut(s) 706, 3367
KspAI GTTAAC 2 cut(s) 1134, 2809
LguI GCTCTTC 4 cut(s) 1155, 2604, 2896, 3411
LmnI GCTCC 3 cut(s) 941, 1207, 3498
Lsp1109I GCAGC 5 cut(s) 100, 430, 722, 809, 1111
LweI GCATC 4 cut(s) 1353, 2390, 2583, 3419
MaeI CTAG 6 cut(s) 866, 1341, 1460, 1617, 2033, 3272
MaeII ACGT 5 cut(s) 639, 837, 2805, 3148, 3573
MaeIII GTNAC 6 cut(s) 136, 803, 1593, 2479, 2672, 3278
MfeI CAATTG 2 cut(s) 201, 687
MflI RGATCY 7 cut(s) 340, 654, 1853, 2035, 2317, 2659, 3234
MhlI GDGCHC 3 cut(s) 700, 3495, 3522
MlsI TGGCCA 1 cut(s) 461
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 9 cut(s) 218, 906, 1601, 1818, 2134, 2143, 2168, 2276, 3371
MmeI TCCRAC 6 cut(s) 858, 1554, 2059, 2421, 2798, 2847
Mox20I TGGCCA 1 cut(s) 461
Mph1103I ATGCAT 3 cut(s) 495, 1551, 3137
MroNI GCCGGC 2 cut(s) 2452, 3325
MroXI GAANNNNTTC 5 cut(s) 885, 1149, 1677, 2553, 3057
MscI TGGCCA 1 cut(s) 461
MslI CAYNNNNRTG 6 cut(s) 108, 323, 633, 1276, 1691, 2544
Msp20I TGGCCA 1 cut(s) 461
MspCI CTTAAG 1 cut(s) 1849
MspI CCGG 5 cut(s) 1055, 2420, 2453, 2626, 3326
MspR9I CCNGG 5 cut(s) 1056, 1605, 3075, 3356, 3468
MunI CAATTG 2 cut(s) 201, 687
Mva1269I GAATGC 2 cut(s) 669, 1736
MvaI CCWGG 4 cut(s) 1605, 3075, 3356, 3468
MwoI GCNNNNNNNGC 5 cut(s) 1555, 1803, 2462, 2794, 3185
NaeI GCCGGC 2 cut(s) 2454, 3327
NciI CCSGG 1 cut(s) 1056
NcoI CCATGG 1 cut(s) 658
NgoMIV GCCGGC 2 cut(s) 2452, 3325
NlaIV GGNNCC 4 cut(s) 656, 1727, 2418, 3494
NmuCI GTSAC 4 cut(s) 136, 803, 1593, 2672
NsbI TGCGCA 1 cut(s) 644
NsiI ATGCAT 3 cut(s) 495, 1551, 3137
NspI RCATGY 3 cut(s) 493, 1412, 1553
NspV TTCGAA 1 cut(s) 1872
PaeI GCATGC 1 cut(s) 1553
PagI TCATGA 2 cut(s) 1039, 1630
PceI AGGCCT 1 cut(s) 383
PciI ACATGT 1 cut(s) 1408
PciSI GCTCTTC 4 cut(s) 1155, 2604, 2896, 3411
PctI GAATGC 2 cut(s) 669, 1736
PdiI GCCGGC 2 cut(s) 2454, 3327
PdmI GAANNNNTTC 5 cut(s) 885, 1149, 1677, 2553, 3057
PfeI GAWTC 7 cut(s) 2107, 2206, 2335, 2528, 2561, 2939, 3119
PflMI CCANNNNNTGG 1 cut(s) 2784
PfoI TCCNGGA 2 cut(s) 1054, 3073
PkrI GCNGC 6 cut(s) 115, 445, 737, 824, 1126, 2990
PleI GAGTC 9 cut(s) 217, 905, 1600, 1818, 2133, 2142, 2167, 2276, 3370
PpsI GAGTC 9 cut(s) 217, 905, 1600, 1818, 2133, 2142, 2167, 2276, 3370
Ppu21I YACGTR 1 cut(s) 3574
PscI ACATGT 1 cut(s) 1408
PshAI GACNNNNGTC 1 cut(s) 156
PshBI ATTAAT 2 cut(s) 2361, 2388
Psp124BI GAGCTC 1 cut(s) 700
Psp1406I AACGTT 2 cut(s) 837, 2805
Psp6I CCWGG 4 cut(s) 1603, 3073, 3354, 3466
PspFI CCCAGC 1 cut(s) 1572
PspGI CCWGG 4 cut(s) 1603, 3073, 3354, 3466
PspN4I GGNNCC 4 cut(s) 656, 1727, 2418, 3494
PspPI GGNCC 5 cut(s) 100, 1052, 1268, 2417, 3464
PstI CTGCAG 1 cut(s) 2133
PstNI CAGNNNCTG 1 cut(s) 735
PsuI RGATCY 7 cut(s) 340, 654, 1853, 2035, 2317, 2659, 3234
RsaI GTAC 3 cut(s) 1615, 2294, 3576
RsaNI GTAC 3 cut(s) 1614, 2293, 3575
RseI CAYNNNNRTG 6 cut(s) 108, 323, 633, 1276, 1691, 2544
Rsr2I CGGWCCG 1 cut(s) 1052
RsrII CGGWCCG 1 cut(s) 1052
SacI GAGCTC 1 cut(s) 700
SalI GTCGAC 1 cut(s) 1781
SapI GCTCTTC 4 cut(s) 1155, 2604, 2896, 3411
SatI GCNGC 6 cut(s) 114, 444, 736, 823, 1125, 2989
Sau96I GGNCC 5 cut(s) 100, 1052, 1268, 2417, 3464
SchI GAGTC 9 cut(s) 218, 906, 1601, 1818, 2134, 2143, 2168, 2276, 3371
ScrFI CCNGG 5 cut(s) 1056, 1605, 3075, 3356, 3468
SduI GDGCHC 3 cut(s) 700, 3495, 3522
SfaNI GCATC 4 cut(s) 1353, 2390, 2583, 3419
SfcI CTRYAG 1 cut(s) 2129
SfuI TTCGAA 1 cut(s) 1872
SinI GGWCC 4 cut(s) 100, 1052, 1268, 2417
SmiMI CAYNNNNRTG 6 cut(s) 108, 323, 633, 1276, 1691, 2544
SmlI CTYRAG 7 cut(s) 161, 901, 1819, 1849, 2024, 2798, 3230
SmoI CTYRAG 7 cut(s) 161, 901, 1819, 1849, 2024, 2798, 3230
SnaBI TACGTA 1 cut(s) 3574
SphI GCATGC 1 cut(s) 1553
SseBI AGGCCT 1 cut(s) 383
SsiI CCGC 6 cut(s) 85, 667, 1190, 2434, 2989, 3489
SspI AATATT 2 cut(s) 1222, 2386
SspMI CTAG 6 cut(s) 866, 1341, 1460, 1617, 2033, 3272
SstI GAGCTC 1 cut(s) 700
StuI AGGCCT 1 cut(s) 383
StyD4I CCNGG 5 cut(s) 1054, 1603, 3073, 3354, 3466
StyI CCWWGG 1 cut(s) 658
TaaI ACNGT 7 cut(s) 546, 731, 792, 809, 2120, 3374, 3447
TaiI ACGT 5 cut(s) 642, 840, 2808, 3151, 3576
TaqI TCGA 6 cut(s) 1289, 1314, 1782, 1830, 1872, 2571
TaqII GACCGA 3 cut(s) 1040, 2488, 2997
TauI GCSGC 1 cut(s) 2991
TfiI GAWTC 7 cut(s) 2107, 2206, 2335, 2528, 2561, 2939, 3119
TscAI CASTG 2 cut(s) 330, 1084
TseFI GTSAC 4 cut(s) 136, 803, 1593, 2672
TseI GCWGC 5 cut(s) 113, 443, 735, 822, 1124
Tsp45I GTSAC 4 cut(s) 136, 803, 1593, 2672
TspGWI ACGGA 6 cut(s) 581, 1763, 2065, 3485, 3573, 3582
TspRI CASTG 2 cut(s) 330, 1084
Van91I CCANNNNNTGG 1 cut(s) 2784
Vha464I CTTAAG 1 cut(s) 1849
VneI GTGCAC 1 cut(s) 3518
VpaK11BI GGWCC 4 cut(s) 100, 1052, 1268, 2417
VspI ATTAAT 2 cut(s) 2361, 2388
XagI CCTNNNNNAGG 2 cut(s) 1602, 3029
XapI RAATTY 7 cut(s) 91, 215, 1398, 1867, 2901, 3084, 3347
XbaI TCTAGA 1 cut(s) 2032
XceI RCATGY 3 cut(s) 493, 1412, 1553
XcmI CCANNNNNNNNNTGG 4 cut(s) 2075, 2297, 2781, 3166
XmiI GTMKAC 3 cut(s) 1782, 2137, 3541
XmnI GAANNNNTTC 5 cut(s) 885, 1149, 1677, 2553, 3057
XspI CTAG 6 cut(s) 866, 1341, 1460, 1617, 2033, 3272
Zsp2I ATGCAT 3 cut(s) 495, 1551, 3137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.