Rroxscaffold_4G00327140

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
59637307 .. 59645236
7930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327140.1

Sequence Viewer

Length: 2616 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGCGGGGAAGATACCCGCAAGGGTTTTACCCGTGATCTGTATAAAATACTAAATGAGAAAGGAATTCTCAGCTTTATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAAGCCCGAACTTTTAGCTGCAATTGAGGACTCTAGATCTGCGATTGTCGTCCTCTCACCCAGATATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATCCAATGCATGAAAGAGATGGGCCAACAAGTCTTGCCCGTCTTCTTGTGGGTGGATCCTTCTGATGTGCGGCACCAAACGGGAAGTTTTGAGCTCAAAACGGAACACCAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATCAGGACAGACTAAATGTCTGGAGAGCTGCTTTGACAGAGGTCGCCAATCTTTCTGGCTGGGTTTCCAAGAATTATTCCTTAAAAACAGATCTAATTAAGGATATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTCATGATTTATTAGGCAATTACATATATCCGCAGCTTGTTGGGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATCATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGGCATTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAGGGCTTTTAAGAAATGTCCCCCACCACAAGATTATTTGGATCTGTGCTACCATATTCTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCGCTGATAAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGACAAACGCATTATTGGGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTGCATATTGCTTGCTTTTACAAGGGAAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGACCCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTTGATGCATGATTTACTACAAGCAATGGGCTGGGAAATTGTTCGTGAACTGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCTTGAAGACATCAACAATGTGCTGAATAGAAATACGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGCCATCTACCCATGATCGCAAGAAAGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCGTCTACCCATGATCGCAAGAAAAAAGGGAGAGGTCCAATCTTTCAAGGATGCAAAGTAGTTTGGCGCTCTTTCAAAAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAGAGAGCTTCCACTTGCCTATATCACAAAACGTGAATGCAGAGCCAAAGAGTTTCCACTTGCCTATATCTGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGATTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTTAACAGACTAGACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAACGGCAACCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAAGAGTGAAACTTGTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCCGCCATCACAGTTATGGGTTTTCTGTAAGTGTATATCTTCCTGCATATGGGAATAACAGTCGGCTAATGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATAAGTACCATCTGGTGGATAAGCTTCCTAGAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCTTGAAGCTTGATGGAAGAAAATTGGAAGTATATGGCAAACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGAGTGTTGGCATAACAGTTGCAGTCAGCTTGAGTTCTTATTTAAAACCAGAGGGCCCGGGTCTGAAGGGGAAACCGGTGGGACTGGTAGCAGAACTTGCACACTTGAAGAATTATCATGAATGGACAATGCCTCCTCTTCTCTCATTTGAATTTCTGCAGAAATTACTTTCTGGAGCTCTCATGCCTTCATCTTTTACAGTTAGTGTATATGATGAAGATACTGCTGTTAAAGGTATTCCATTTGATATAGATAGCGAGGATCGAGGCAGTACAGGTGTACAAAGGTTACTTGAACTTTCCTCGAGTAGTGCAAATTCATGTGGACTTGATCAGCTTACTTTCCTCTTTACGCTTTCATTAATGAAACTTGAATTCATCAAGAAGGGGGGAAATAATTTGATGTTGGCTTTAACTACAGTTGGCTTAAAGGTGGAATTAACTGGTAGAAGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

871

Amino Acids

97.89

Weight (kDa)

7.77

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 189 2.5e-51 TIR domain
TIR_2 PF13676 14 - 110 2.4e-15 TIR domain
WHD_ROQ1 PF23282 337 - 408 6.1e-21 Disease resistance protein Roq1-like, winged-helix domain
C-JID PF20160 627 - 691 2.3e-11 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 1 cut(s) 2023
AccI GTMKAC 3 cut(s) 554, 1397, 1672
AciI CCGC 6 cut(s) 57, 70, 340, 598, 632, 1909
AclWI GGATC 5 cut(s) 320, 333, 843, 1077, 2427
AcsI RAATTY 7 cut(s) 117, 1729, 2045, 2150, 2309, 2473, 2531
AcuI CTGAAG 2 cut(s) 1844, 2243
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 1699, 2004, 2015, 2431, 2439
AfeI AGCGCT 1 cut(s) 919
AfiI CCNNNNNNNGG 4 cut(s) 1115, 1583, 1955, 2023
AgeI ACCGGT 1 cut(s) 2233
AhdI GACNNNNNGTC 1 cut(s) 434
AjnI CCWGG 3 cut(s) 676, 1234, 1882
AjuI GAANNNNNNNTTGG 2 cut(s) 1604, 1636
Alw21I GWGCWC 2 cut(s) 366, 2338
Alw26I GTCTC 3 cut(s) 1227, 1263, 1630
AlwI GGATC 5 cut(s) 320, 333, 843, 1077, 2427
Ama87I CYCGRG 2 cut(s) 2215, 2461
Aor51HI AGCGCT 1 cut(s) 919
AoxI GGCC 2 cut(s) 292, 2212
ApaI GGGCCC 1 cut(s) 2216
ApeKI GCWGC 3 cut(s) 182, 446, 634
ApoI RAATTY 7 cut(s) 117, 1729, 2045, 2150, 2309, 2473, 2531
AseI ATTAAT 1 cut(s) 2519
AsiGI ACCGGT 1 cut(s) 2233
Asp700I GAANNNNTTC 4 cut(s) 592, 777, 1209, 1829
AspLEI GCGC 2 cut(s) 920, 1461
AspS9I GGNCC 5 cut(s) 292, 674, 1427, 2212, 2213
AsuC2I CCSGG 2 cut(s) 2216, 2217
AsuHPI GGTGA 1 cut(s) 213
AvaI CYCGRG 2 cut(s) 2215, 2461
AvaII GGWCC 2 cut(s) 674, 1427
BaeGI GKGCMC 1 cut(s) 2216
BaeI ACNNNNGTAYC 2 cut(s) 2370, 2403
BamHI GGATCC 1 cut(s) 325
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 3 cut(s) 366, 2216, 2338
BauI CACGAG 1 cut(s) 884
BbsI GAAGAC 3 cut(s) 304, 1272, 1832
Bbv12I GWGCWC 2 cut(s) 366, 2338
BbvI GCAGC 3 cut(s) 169, 433, 646
BccI CCATC 8 cut(s) 283, 995, 1342, 1669, 1685, 1920, 2025, 2090
BceAI ACGGC 1 cut(s) 1801
BciT130I CCWGG 3 cut(s) 678, 1236, 1884
BclI TGATCA 1 cut(s) 2488
BcnI CCSGG 2 cut(s) 2216, 2217
BcoDI GTCTC 3 cut(s) 1227, 1263, 1630
BfaI CTAG 7 cut(s) 198, 854, 1088, 1103, 1676, 1742, 2037
BfmI CTRYAG 2 cut(s) 2315, 2574
BfoI RGCGCY 2 cut(s) 921, 1462
BglII AGATCT 3 cut(s) 200, 508, 1139
BisI GCNGC 4 cut(s) 183, 341, 447, 635
BlsI GCNGC 4 cut(s) 184, 342, 448, 636
Bme1390I CCNGG 5 cut(s) 678, 1236, 1884, 2216, 2217
Bme18I GGWCC 2 cut(s) 674, 1427
BmeRI GACNNNNNGTC 1 cut(s) 434
BmeT110I CYCGRG 2 cut(s) 2215, 2461
BmgT120I GGNCC 5 cut(s) 292, 674, 1427, 2212, 2213
BmiI GGNNCC 4 cut(s) 327, 344, 1907, 2214
BmrFI CCNGG 5 cut(s) 678, 1236, 1884, 2216, 2217
BmsI GCATC 6 cut(s) 727, 1164, 1433, 1711, 1733, 1787
BoxI GACNNNNGTC 1 cut(s) 458
BpiI GAAGAC 3 cut(s) 304, 1272, 1832
BpmI CTGGAG 2 cut(s) 460, 2352
Bpu10I CCTNAGC 1 cut(s) 864
BpuEI CTTGAG 3 cut(s) 167, 1628, 2209
BpuMI CCSGG 2 cut(s) 2216, 2217
BsaBI GATNNNNATC 1 cut(s) 1068
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1263
BsaJI CCNNGG 3 cut(s) 677, 1792, 2215
BsaWI WCCGGW 1 cut(s) 2233
BsaXI ACNNNNNCTCC 2 cut(s) 2275, 2305
Bsc4I CCNNNNNNNGG 4 cut(s) 1115, 1583, 1955, 2023
Bse118I RCCGGY 1 cut(s) 2233
Bse1I ACTGG 4 cut(s) 671, 1011, 2247, 2605
Bse3DI GCAATG 1 cut(s) 1200
Bse8I GATNNNNATC 1 cut(s) 1068
BseBI CCWGG 3 cut(s) 678, 1236, 1884
BseDI CCNNGG 3 cut(s) 677, 1792, 2215
BseGI GGATG 5 cut(s) 139, 259, 1015, 1448, 1802
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 4 cut(s) 1115, 1583, 1955, 2023
BseMI GCAATG 1 cut(s) 1200
BseMII CTCAG 3 cut(s) 136, 878, 1890
BseNI ACTGG 4 cut(s) 671, 1011, 2247, 2605
BseRI GAGGAG 1 cut(s) 2283
BseSI GKGCMC 1 cut(s) 2216
BseXI GCAGC 3 cut(s) 169, 433, 646
BseYI CCCAGC 2 cut(s) 477, 1200
BshFI GGCC 2 cut(s) 294, 2214
BshNI GGYRCC 1 cut(s) 342
BshTI ACCGGT 1 cut(s) 2233
BsiHKAI GWGCWC 2 cut(s) 366, 2338
BsiHKCI CYCGRG 2 cut(s) 2215, 2461
BsiSI CCGG 2 cut(s) 2216, 2234
BslFI GGGAC 2 cut(s) 798, 2253
BslI CCNNNNNNNGG 4 cut(s) 1115, 1583, 1955, 2023
BsmAI GTCTC 3 cut(s) 1227, 1263, 1630
BsmFI GGGAC 2 cut(s) 798, 2253
BsmI GAATGC 2 cut(s) 600, 1555
BsnI GGCC 2 cut(s) 294, 2214
Bso31I GGTCTC 1 cut(s) 1263
BsoBI CYCGRG 2 cut(s) 2215, 2461
Bsp120I GGGCCC 1 cut(s) 2212
Bsp1286I GDGCHC 3 cut(s) 366, 2216, 2338
Bsp1407I TGTACA 1 cut(s) 2437
BspACI CCGC 6 cut(s) 57, 70, 340, 598, 632, 1909
BspANI GGCC 2 cut(s) 294, 2214
BspCNI CTCAG 3 cut(s) 135, 877, 1891
BspHI TCATGA 2 cut(s) 604, 2275
BspLI GGNNCC 4 cut(s) 327, 344, 1907, 2214
BspMAI CTGCAG 1 cut(s) 2319
BspPI GGATC 5 cut(s) 320, 333, 843, 1077, 2427
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 1 cut(s) 1263
BsrDI GCAATG 1 cut(s) 1200
BsrFI RCCGGY 1 cut(s) 2233
BsrGI TGTACA 1 cut(s) 2437
BsrI ACTGG 4 cut(s) 671, 1011, 2247, 2605
BssAI RCCGGY 1 cut(s) 2233
BssECI CCNNGG 3 cut(s) 677, 1792, 2215
BssNAI GTATAC 2 cut(s) 555, 1673
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 884
BssT1I CCWWGG 1 cut(s) 1792
Bst1107I GTATAC 2 cut(s) 555, 1673
Bst2BI CACGAG 1 cut(s) 884
Bst2UI CCWGG 3 cut(s) 678, 1236, 1884
Bst4CI ACNGT 8 cut(s) 767, 1170, 1221, 1919, 1967, 2176, 2359, 2578
Bst6I CTCTTC 2 cut(s) 19, 2301
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 2255
BstAUI TGTACA 1 cut(s) 2437
BstC8I GCNNGC 3 cut(s) 1045, 1100, 2130
BstDEI CTNAG 5 cut(s) 47, 122, 864, 1602, 1899
BstF5I GGATG 5 cut(s) 139, 259, 1015, 1448, 1802
BstH2I RGCGCY 2 cut(s) 921, 1462
BstHHI GCGC 2 cut(s) 920, 1461
BstMAI GTCTC 3 cut(s) 1227, 1263, 1630
BstMWI GCNNNNNNNGC 3 cut(s) 1603, 1991, 2255
BstNI CCWGG 3 cut(s) 678, 1236, 1884
BstPAI GACNNNNGTC 1 cut(s) 458
BstSCI CCNGG 5 cut(s) 676, 1234, 1882, 2214, 2215
BstSFI CTRYAG 2 cut(s) 2315, 2574
BstSLI GKGCMC 1 cut(s) 2216
BstV1I GCAGC 3 cut(s) 169, 433, 646
BstV2I GAAGAC 3 cut(s) 304, 1272, 1832
BstX2I RGATCY 5 cut(s) 200, 325, 508, 835, 1139
BstXI CCANNNNNNTGG 1 cut(s) 2165
BstYI RGATCY 5 cut(s) 200, 325, 508, 835, 1139
BstZ17I GTATAC 2 cut(s) 555, 1673
BsuRI GGCC 2 cut(s) 294, 2214
BtsCI GGATG 5 cut(s) 139, 259, 1015, 1448, 1802
BtsIMutI CAGTG 1 cut(s) 1703
Cac8I GCNNGC 3 cut(s) 1045, 1100, 2130
CciI TCATGA 2 cut(s) 604, 2275
CfoI GCGC 2 cut(s) 920, 1461
Cfr10I RCCGGY 1 cut(s) 2233
Cfr13I GGNCC 5 cut(s) 292, 674, 1427, 2212, 2213
Cfr9I CCCGGG 1 cut(s) 2215
CseI GACGC 1 cut(s) 146
Csp6I GTAC 5 cut(s) 1698, 2003, 2014, 2430, 2438
CspAI ACCGGT 1 cut(s) 2233
CviQI GTAC 5 cut(s) 1698, 2003, 2014, 2430, 2438
DdeI CTNAG 5 cut(s) 47, 122, 864, 1602, 1899
DraI TTTAAA 1 cut(s) 2202
DriI GACNNNNNGTC 1 cut(s) 434
Eam1104I CTCTTC 2 cut(s) 19, 2301
Eam1105I GACNNNNNGTC 1 cut(s) 434
EarI CTCTTC 2 cut(s) 19, 2301
EciI GGCGGA 1 cut(s) 1898
Ecl136II GAGCTC 2 cut(s) 364, 2336
Eco130I CCWWGG 1 cut(s) 1792
Eco24I GRGCYC 3 cut(s) 366, 2216, 2338
Eco31I GGTCTC 1 cut(s) 1263
Eco47I GGWCC 2 cut(s) 674, 1427
Eco47III AGCGCT 1 cut(s) 919
Eco53kI GAGCTC 2 cut(s) 364, 2336
Eco57I CTGAAG 2 cut(s) 1844, 2243
Eco88I CYCGRG 2 cut(s) 2215, 2461
EcoICRI GAGCTC 2 cut(s) 364, 2336
EcoO109I RGGNCCY 1 cut(s) 2212
EcoRI GAATTC 3 cut(s) 117, 2045, 2531
EcoRII CCWGG 3 cut(s) 676, 1234, 1882
EcoT14I CCWWGG 1 cut(s) 1792
EcoT22I ATGCAT 3 cut(s) 281, 1179, 1726
EcoT38I GRGCYC 3 cut(s) 366, 2216, 2338
ErhI CCWWGG 1 cut(s) 1792
FaqI GGGAC 2 cut(s) 798, 2253
FauI CCCGC 3 cut(s) 50, 77, 605
FauNDI CATATG 1 cut(s) 1954
FbaI TGATCA 1 cut(s) 2488
FblI GTMKAC 3 cut(s) 554, 1397, 1672
Fnu4HI GCNGC 4 cut(s) 183, 341, 447, 635
FokI GGATG 5 cut(s) 146, 266, 1022, 1455, 1809
FriOI GRGCYC 3 cut(s) 366, 2216, 2338
Fsp4HI GCNGC 4 cut(s) 183, 341, 447, 635
FspBI CTAG 7 cut(s) 198, 854, 1088, 1103, 1676, 1742, 2037
GlaI GCGC 2 cut(s) 919, 1460
GluI GCNGC 4 cut(s) 183, 341, 447, 635
GsaI CCCAGC 2 cut(s) 481, 1204
GsuI CTGGAG 2 cut(s) 460, 2352
HaeII RGCGCY 2 cut(s) 921, 1462
HaeIII GGCC 2 cut(s) 294, 2214
HapII CCGG 2 cut(s) 2216, 2234
HgaI GACGC 1 cut(s) 146
HhaI GCGC 2 cut(s) 920, 1461
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 918, 1459
HinP1I GCGC 2 cut(s) 918, 1459
HincII GTYRAC 2 cut(s) 9, 571
HindII GTYRAC 2 cut(s) 9, 571
HindIII AAGCTT 3 cut(s) 1604, 2030, 2090
HinfI GANTC 5 cut(s) 194, 418, 593, 687, 1845
HpaII CCGG 2 cut(s) 2216, 2234
HphI GGTGA 1 cut(s) 213
Hpy166II GTNNAC 8 cut(s) 9, 555, 571, 1217, 1398, 1673, 2438, 2483
Hpy188I TCNGA 4 cut(s) 334, 990, 1654, 2223
Hpy8I GTNNAC 8 cut(s) 9, 555, 571, 1217, 1398, 1673, 2438, 2483
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 6 cut(s) 339, 1491, 1667, 2218, 2355, 2536
HpyCH4III ACNGT 8 cut(s) 767, 1170, 1221, 1919, 1967, 2176, 2359, 2578
HpyCH4IV ACGT 2 cut(s) 1243, 1545
HpyF10VI GCNNNNNNNGC 3 cut(s) 1603, 1991, 2255
HpyF3I CTNAG 5 cut(s) 47, 122, 864, 1602, 1899
HpySE526I ACGT 2 cut(s) 1243, 1545
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 918, 1459
Ksp22I TGATCA 1 cut(s) 2488
LmnI GCTCC 1 cut(s) 2333
Lsp1109I GCAGC 3 cut(s) 169, 433, 646
LweI GCATC 6 cut(s) 727, 1164, 1433, 1711, 1733, 1787
MaeI CTAG 7 cut(s) 198, 854, 1088, 1103, 1676, 1742, 2037
MaeII ACGT 2 cut(s) 1243, 1545
MaeIII GTNAC 2 cut(s) 1075, 2445
MfeI CAATTG 2 cut(s) 186, 1768
MflI RGATCY 5 cut(s) 200, 325, 508, 835, 1139
MhlI GDGCHC 3 cut(s) 366, 2216, 2338
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 1182
Mph1103I ATGCAT 3 cut(s) 281, 1179, 1726
MroXI GAANNNNTTC 4 cut(s) 592, 777, 1209, 1829
MslI CAYNNNNRTG 2 cut(s) 587, 1920
MspA1I CMGCKG 1 cut(s) 57
MspI CCGG 2 cut(s) 2216, 2234
MspR9I CCNGG 5 cut(s) 678, 1236, 1884, 2216, 2217
MunI CAATTG 2 cut(s) 186, 1768
Mva1269I GAATGC 2 cut(s) 600, 1555
MvaI CCWGG 3 cut(s) 678, 1236, 1884
MwoI GCNNNNNNNGC 3 cut(s) 1603, 1991, 2255
NciI CCSGG 2 cut(s) 2216, 2217
NdeI CATATG 1 cut(s) 1954
NlaIV GGNNCC 4 cut(s) 327, 344, 1907, 2214
NmuCI GTSAC 1 cut(s) 1075
NsiI ATGCAT 3 cut(s) 281, 1179, 1726
PaeR7I CTCGAG 1 cut(s) 2461
PagI TCATGA 2 cut(s) 604, 2275
PctI GAATGC 2 cut(s) 600, 1555
PdmI GAANNNNTTC 4 cut(s) 592, 777, 1209, 1829
PfeI GAWTC 4 cut(s) 418, 593, 687, 1845
PflFI GACNNNGTC 1 cut(s) 1109
PflMI CCANNNNNTGG 1 cut(s) 2023
PfoI TCCNGGA 1 cut(s) 1882
PinAI ACCGGT 1 cut(s) 2233
PkrI GCNGC 4 cut(s) 184, 342, 448, 636
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PshAI GACNNNNGTC 1 cut(s) 458
PshBI ATTAAT 1 cut(s) 2519
Psp124BI GAGCTC 2 cut(s) 366, 2338
Psp6I CCWGG 3 cut(s) 676, 1234, 1882
PspFI CCCAGC 2 cut(s) 477, 1200
PspGI CCWGG 3 cut(s) 676, 1234, 1882
PspN4I GGNNCC 4 cut(s) 327, 344, 1907, 2214
PspOMI GGGCCC 1 cut(s) 2212
PspPI GGNCC 5 cut(s) 292, 674, 1427, 2212, 2213
PspXI VCTCGAGB 1 cut(s) 2461
PstI CTGCAG 1 cut(s) 2319
PsuI RGATCY 5 cut(s) 200, 325, 508, 835, 1139
PsyI GACNNNGTC 1 cut(s) 1109
RsaI GTAC 5 cut(s) 1699, 2004, 2015, 2431, 2439
RsaNI GTAC 5 cut(s) 1698, 2003, 2014, 2430, 2438
RseI CAYNNNNRTG 2 cut(s) 587, 1920
SacI GAGCTC 2 cut(s) 366, 2338
SatI GCNGC 4 cut(s) 183, 341, 447, 635
Sau96I GGNCC 5 cut(s) 292, 674, 1427, 2212, 2213
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 5 cut(s) 678, 1236, 1884, 2216, 2217
SduI GDGCHC 3 cut(s) 366, 2216, 2338
SfaNI GCATC 6 cut(s) 727, 1164, 1433, 1711, 1733, 1787
SfcI CTRYAG 2 cut(s) 2315, 2574
Sfr274I CTCGAG 1 cut(s) 2461
SinI GGWCC 2 cut(s) 674, 1427
SlaI CTCGAG 1 cut(s) 2461
SmaI CCCGGG 1 cut(s) 2217
SmiMI CAYNNNNRTG 2 cut(s) 587, 1920
SmlI CTYRAG 4 cut(s) 146, 1607, 2188, 2461
SmoI CTYRAG 4 cut(s) 146, 1607, 2188, 2461
SsiI CCGC 6 cut(s) 57, 70, 340, 598, 632, 1909
SspI AATATT 2 cut(s) 1029, 1894
SspMI CTAG 7 cut(s) 198, 854, 1088, 1103, 1676, 1742, 2037
SstI GAGCTC 2 cut(s) 366, 2338
StyD4I CCNGG 5 cut(s) 676, 1234, 1882, 2214, 2215
StyI CCWWGG 1 cut(s) 1792
TaaI ACNGT 8 cut(s) 767, 1170, 1221, 1919, 1967, 2176, 2359, 2578
TaiI ACGT 2 cut(s) 1246, 1548
TaqI TCGA 2 cut(s) 2422, 2462
TatI WGTACW 4 cut(s) 1697, 2002, 2429, 2437
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 4 cut(s) 418, 593, 687, 1845
TscAI CASTG 1 cut(s) 1710
TseFI GTSAC 1 cut(s) 1075
TseI GCWGC 3 cut(s) 182, 446, 634
Tsp45I GTSAC 1 cut(s) 1075
TspGWI ACGGA 4 cut(s) 386, 538, 864, 1310
TspMI CCCGGG 1 cut(s) 2215
TspRI CASTG 1 cut(s) 1710
Tth111I GACNNNGTC 1 cut(s) 1109
Van91I CCANNNNNTGG 1 cut(s) 2023
VpaK11BI GGWCC 2 cut(s) 674, 1427
VspI ATTAAT 1 cut(s) 2519
XapI RAATTY 7 cut(s) 117, 1729, 2045, 2150, 2309, 2473, 2531
XbaI TCTAGA 1 cut(s) 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 1919
XhoI CTCGAG 1 cut(s) 2461
XmaI CCCGGG 1 cut(s) 2215
XmiI GTMKAC 3 cut(s) 554, 1397, 1672
XmnI GAANNNNTTC 4 cut(s) 592, 777, 1209, 1829
XspI CTAG 7 cut(s) 198, 854, 1088, 1103, 1676, 1742, 2037
Zsp2I ATGCAT 3 cut(s) 281, 1179, 1726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.