RchiOBHm_Chr1g0328951

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
18402490 .. 18403826
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55830

Sequence Viewer

Length: 933 bp
ATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAGCAGTCTCCTGATGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGACGACATCAACAATGTGCTGAAGAAAAATGACGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAACTACAAGTTGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTGGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCAAATGGACTGGGTATCCCTTAAGATCTCTCCCAAAACTTTTTGAAGCAGACAAACTTATTGAACTTAACTTGTGCCACAGCAACGTTAAACAGCTTTGGAAGGGAACGAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAAAATTGTGGAGACCCCCGACCTCACAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTTTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAGAGTCTGAAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGACATTGAATCTAAGCAACTGCAGAAATCTTGTTTGTCTTCCAAGGACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACTTCTGGTAAGTGTGTGTGTGTGTGTGTGGGGGGGGGGGGGGGGTGGACTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

310

Amino Acids

34.91

Weight (kDa)

8.66

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 206 - 291 7.8e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 217 - 285 3.7e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 145
AclI AACGTT 2 cut(s) 372, 691
AcsI RAATTY 2 cut(s) 202, 400
AcuI CTGAAG 2 cut(s) 131, 608
AfiI CCNNNNNNNGG 1 cut(s) 145
AflII CTTAAG 1 cut(s) 307
AjnI CCWGG 1 cut(s) 61
AluBI AGCT 2 cut(s) 382, 553
AluI AGCT 2 cut(s) 382, 553
Alw26I GTCTC 7 cut(s) 54, 90, 213, 274, 446, 479, 710
Ama87I CYCGRG 1 cut(s) 668
AoxI GGCC 2 cut(s) 253, 760
ApoI RAATTY 2 cut(s) 202, 400
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 2 cut(s) 36, 130
AspS9I GGNCC 2 cut(s) 241, 813
AvaI CYCGRG 1 cut(s) 668
AvaII GGWCC 2 cut(s) 241, 813
BbsI GAAGAC 2 cut(s) 212, 797
BccI CCATC 2 cut(s) 534, 824
BceAI ACGGC 1 cut(s) 767
BciT130I CCWGG 1 cut(s) 63
BciVI GTATCC 1 cut(s) 312
BcoDI GTCTC 7 cut(s) 54, 90, 213, 274, 446, 479, 710
BfmI CTRYAG 1 cut(s) 787
BfrI CTTAAG 1 cut(s) 307
BfuI GTATCC 1 cut(s) 312
BglII AGATCT 2 cut(s) 311, 493
Bme1390I CCNGG 1 cut(s) 63
Bme18I GGWCC 2 cut(s) 241, 813
BmeT110I CYCGRG 1 cut(s) 668
BmgT120I GGNCC 2 cut(s) 241, 813
BmrFI CCNGG 1 cut(s) 63
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 1 cut(s) 848
BmuI ACTGGG 1 cut(s) 306
BpiI GAAGAC 2 cut(s) 212, 797
BpuEI CTTGAG 2 cut(s) 298, 503
BsaI GGTCTC 2 cut(s) 90, 446
BsaJI CCNNGG 3 cut(s) 139, 535, 809
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 2 cut(s) 301, 763
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 3 cut(s) 139, 535, 809
BseGI GGATG 2 cut(s) 509, 863
BseLI CCNNNNNNNGG 1 cut(s) 145
BseNI ACTGG 2 cut(s) 301, 763
BseRI GAGGAG 1 cut(s) 275
BseYI CCCAGC 1 cut(s) 27
BshFI GGCC 2 cut(s) 255, 762
BsiHKCI CYCGRG 1 cut(s) 668
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 7 cut(s) 54, 90, 213, 274, 446, 479, 710
BsnI GGCC 2 cut(s) 255, 762
Bso31I GGTCTC 2 cut(s) 90, 446
BsoBI CYCGRG 1 cut(s) 668
Bsp143I GATC 2 cut(s) 311, 493
BspANI GGCC 2 cut(s) 255, 762
BspHI TCATGA 1 cut(s) 88
BspMAI CTGCAG 1 cut(s) 791
BspTI CTTAAG 1 cut(s) 307
BspTNI GGTCTC 2 cut(s) 90, 446
BsrI ACTGG 2 cut(s) 301, 763
BssECI CCNNGG 3 cut(s) 139, 535, 809
BssMI GATC 2 cut(s) 311, 493
BssT1I CCWWGG 3 cut(s) 139, 535, 809
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 1 cut(s) 927
Bst6I CTCTTC 1 cut(s) 584
BstAFI CTTAAG 1 cut(s) 307
BstAPI GCANNNNNTGC 1 cut(s) 10
BstDEI CTNAG 1 cut(s) 779
BstF5I GGATG 2 cut(s) 509, 863
BstKTI GATC 2 cut(s) 314, 496
BstMAI GTCTC 7 cut(s) 54, 90, 213, 274, 446, 479, 710
BstMBI GATC 2 cut(s) 311, 493
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 787
BstV2I GAAGAC 2 cut(s) 212, 797
BstX2I RGATCY 2 cut(s) 311, 493
BstYI RGATCY 2 cut(s) 311, 493
BsuI GTATCC 1 cut(s) 312
BsuRI GGCC 2 cut(s) 255, 762
BtsCI GGATG 2 cut(s) 509, 863
CciI TCATGA 1 cut(s) 88
Cfr13I GGNCC 2 cut(s) 241, 813
CviAII CATG 3 cut(s) 5, 89, 145
CviJI RGCY 9 cut(s) 27, 61, 187, 194, 255, 382, 553, 754, 762
CviKI_1 RGCY 9 cut(s) 27, 61, 187, 194, 255, 382, 553, 754, 762
DdeI CTNAG 1 cut(s) 779
DpnI GATC 2 cut(s) 313, 495
DpnII GATC 2 cut(s) 311, 493
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Eco130I CCWWGG 3 cut(s) 139, 535, 809
Eco147I AGGCCT 1 cut(s) 255
Eco31I GGTCTC 2 cut(s) 90, 446
Eco47I GGWCC 2 cut(s) 241, 813
Eco57I CTGAAG 2 cut(s) 131, 608
Eco88I CYCGRG 1 cut(s) 668
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 3 cut(s) 139, 535, 809
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 139, 535, 809
FaeI CATG 3 cut(s) 8, 92, 148
FaiI YATR 6 cut(s) 6, 82, 90, 146, 228, 686
FatI CATG 3 cut(s) 4, 88, 144
FokI GGATG 2 cut(s) 516, 870
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 2 cut(s) 255, 762
Hin1II CATG 3 cut(s) 8, 92, 148
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HindIII AAGCTT 1 cut(s) 551
Hpy166II GTNNAC 3 cut(s) 159, 241, 923
Hpy188I TCNGA 2 cut(s) 477, 588
Hpy8I GTNNAC 3 cut(s) 159, 241, 923
HpyAV CCTTC 3 cut(s) 254, 382, 494
HpyCH4III ACNGT 1 cut(s) 927
HpyCH4IV ACGT 3 cut(s) 70, 372, 691
HpyCH4V TGCA 3 cut(s) 4, 579, 789
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 779
HpySE526I ACGT 3 cut(s) 70, 372, 691
Hsp92II CATG 3 cut(s) 8, 92, 148
Kzo9I GATC 2 cut(s) 311, 493
LweI GCATC 1 cut(s) 848
MaeII ACGT 3 cut(s) 70, 372, 691
MaeIII GTNAC 1 cut(s) 766
MalI GATC 2 cut(s) 313, 495
MboI GATC 2 cut(s) 311, 493
MboII GAAGA 6 cut(s) 124, 212, 409, 601, 673, 797
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 2 cut(s) 311, 493
MluCI AATT 9 cut(s) 33, 202, 271, 400, 444, 606, 633, 744, 824
MlyI GAGTC 3 cut(s) 276, 592, 601
MmeI TCCRAC 1 cut(s) 658
MnlI CCTC 4 cut(s) 266, 296, 473, 721
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 36, 130
MseI TTAA 7 cut(s) 308, 354, 375, 570, 632, 657, 846
MslI CAYNNNNRTG 1 cut(s) 149
MspCI CTTAAG 1 cut(s) 307
MspR9I CCNGG 1 cut(s) 63
MunI CAATTG 1 cut(s) 744
MvaI CCWGG 1 cut(s) 63
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeII GATC 2 cut(s) 311, 493
NlaIII CATG 3 cut(s) 8, 92, 148
NmeAIII GCCGAG 1 cut(s) 849
NmuCI GTSAC 1 cut(s) 766
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 88
PceI AGGCCT 1 cut(s) 255
PdmI GAANNNNTTC 2 cut(s) 36, 130
PfeI GAWTC 7 cut(s) 131, 478, 522, 565, 617, 664, 775
PflMI CCANNNNNTGG 1 cut(s) 145
PleI GAGTC 3 cut(s) 276, 591, 600
PpsI GAGTC 3 cut(s) 276, 591, 600
PshBI ATTAAT 1 cut(s) 632
Psp1406I AACGTT 2 cut(s) 372, 691
Psp6I CCWGG 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 1 cut(s) 61
PspPI GGNCC 2 cut(s) 241, 813
PstI CTGCAG 1 cut(s) 791
PsuI RGATCY 2 cut(s) 311, 493
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 7 cut(s) 308, 354, 375, 570, 632, 657, 846
Sau3AI GATC 2 cut(s) 311, 493
Sau96I GGNCC 2 cut(s) 241, 813
SchI GAGTC 3 cut(s) 276, 592, 601
ScrFI CCNGG 1 cut(s) 63
SetI ASST 8 cut(s) 73, 246, 375, 384, 465, 472, 555, 694
SfaNI GCATC 1 cut(s) 848
SfcI CTRYAG 1 cut(s) 787
SinI GGWCC 2 cut(s) 241, 813
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 3 cut(s) 277, 307, 482
SmoI CTYRAG 3 cut(s) 277, 307, 482
Sse9I AATT 9 cut(s) 33, 202, 271, 400, 444, 606, 633, 744, 824
SseBI AGGCCT 1 cut(s) 255
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 61
StyI CCWWGG 3 cut(s) 139, 535, 809
TaaI ACNGT 1 cut(s) 927
TaiI ACGT 3 cut(s) 73, 375, 694
TasI AATT 9 cut(s) 33, 202, 271, 400, 444, 606, 633, 744, 824
TfiI GAWTC 7 cut(s) 131, 478, 522, 565, 617, 664, 775
Tru1I TTAA 7 cut(s) 308, 354, 375, 570, 632, 657, 846
Tru9I TTAA 7 cut(s) 308, 354, 375, 570, 632, 657, 846
TseFI GTSAC 1 cut(s) 766
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 2 cut(s) 409, 500
TspGWI ACGGA 1 cut(s) 138
Van91I CCANNNNNTGG 1 cut(s) 145
Vha464I CTTAAG 1 cut(s) 307
VpaK11BI GGWCC 2 cut(s) 241, 813
VspI ATTAAT 1 cut(s) 632
XapI RAATTY 2 cut(s) 202, 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 533
XmnI GAANNNNTTC 2 cut(s) 36, 130
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.