Rw1G004830

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Forward (+)
9270524 .. 9273651
3128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004830.1

Sequence Viewer

Length: 2805 bp
ATGTCTAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATACAAAGGCGGTTGCGTAGAATAAAGGTGCTTGTGGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCTGGGAGTAGAATTATCATAACCACCACAGATATTCAGTTATTAAAGGCGCATGATGTTGATGCTCTATACAAGGCTAATGTGTTAAACTATGCTGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAGATGTCCCCCACCAGAAGATTATTTGCATCTGTGCGACCATATTTTAGAGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCCTTTCTATCTGGTAGAGGGGCTGATAAATGGGCAAGCACAATAGATAGGCTAAGGAGCACACCAGATAAAGACATTATTGATGTGCTTCGGATTAGTTTTGATGACCTGCATAAAAAAGAGAGAGAAATATTCCTACATATTGCATGCTTTTACAAGGGAAAGGATAAGGATCTTGTGACACAAATACTAGAATATTGCCAGCTAGAACCTGTCATTGGTTTAGATGTTCTTGTTGATAGATCTCTCATAACTATCTCCAACAACAAACTGTCAATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAATGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGCAGACATCAACAATGTGCTGAAGAGTCATAAGGGAACAGATTCAATCCAAGGCATTGTAATGGAGTTGACTAAATTACAAGTGACTCATTGGAATCCAGAAGCCTTTTCAAATTTATCTCAACTTATTCTTCTCCATATTCGTAATGTGGACCTTCCTGAAGGCCTCACTTGTCTTCCAAATTCCTTGAGACTCCTGGAATGGACTGGGTATCCCTTAAGATCTCTCCCACAAAACTTTGAAGCAGATGATCTCATTGAACTTAACCTGTGCCAGAGCAACATTGCATATCTTTGGAATGGAACAAAGAATTTTGACAGGTTGAAGTTCATCAAACTCTGCCACTCTCAAAACATTGTGGAGACCCCAGACCTCACAGGCGTTCGGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATAAATCCCTTGGATTTCTCAAAAATCTTATTGTTGTGAATCTTAAAGACTGCAAAAGTCTCAAGACTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAGTTCTTTCTAACTGCTCAAAAATTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGTGTCAATTGAAAGGTTGAGTGGCCTGGTGTCATTGAATCTGAGCAACTGCAGAAATCTTGCTCATCTTCCGAGTACCATCAATAAATTGAAGTCTATTAGAAATCTTAATCTTTCTGGATGCTTGAAACTTGGCAAACATCAAGTAAATGTGTGGGAGAGGGACTGTTTTGTGGAAAATGATGTGAATAGTGGGTCTGCAGTAGAAATGTCATCTACCCATGATCTCATAAAATATGTGAGAGGTTCAGTCTTTCGTGGATGCAAAGTAGTTTGGGGATCTGTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAATGAGTTACCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGATTACTTTCCCTCTTTGGTGACCTTAAATCTAAGTGGAAACAATTTTATTCGTCTTCCTTCTGGCATTAGATTGCTTTCTAAGCTTGAGAATTTCAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAAGGCAGATGGTTGTACTTCACTGAAATACCTGTTTGATGCATCAAATATGAACGGATTAAACAAATCATATTTCAATTTCATCATTTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCATATGGTAATTCCAGGAAGTAATATTCCTGAGTGGTTCAGCCATCGGAGTGTTGGGTGTTCATTAAGTATGGGTCCTGCATGCTTAGATGAAGTTTTGGGATTTGCTTTATGTGCTGTTTTTGTACTCCATGAGCACCATCCAGTGGATAAGCTTTATATGGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGTTCAATGGAATAGAATTGGAAGTATATGGCACACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCTGGTTGAATCAGATCACCTGTGGCTATTCTATGTATCTAGTGATAAGTACTTTGGTACAGAGAGGTGGCGTAACTGTTACGGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGATGGTGAGGGAGTGTGGAGTCCGTCTGATGTATGAGGAAGATGTGCAAGTGTTGAACCAAACAACCACTCAATCAAGCAGCAGGATGTCTCCTTATGAGGATATATTGATTGGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGCCACTGGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

934

Amino Acids

105.86

Weight (kDa)

5.87

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 113 1.5e-08 NB-ARC domain
WHD_ROQ1 PF23282 182 - 252 5.2e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 386 - 519 1.6e-09 Leucine-rich repeat region
LRR_13 PF23286 441 - 526 2.5e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 744 - 890 1.2e-12 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 546
AccB7I CCANNNNNTGG 1 cut(s) 2365
AccI GTMKAC 1 cut(s) 2023
AciI CCGC 2 cut(s) 115, 1786
AclI AACGTT 1 cut(s) 1396
AclWI GGATC 3 cut(s) 165, 609, 1729
AcsI RAATTY 8 cut(s) 907, 976, 1105, 1216, 1868, 1963, 2387, 2492
AcuI CTGAAG 5 cut(s) 324, 836, 975, 2195, 2450
AfaI GTAC 5 cut(s) 1519, 2050, 2346, 2549, 2557
AfiI CCNNNNNNNGG 4 cut(s) 647, 1799, 2365, 2753
AflII CTTAAG 1 cut(s) 1012
AjnI CCWGG 6 cut(s) 208, 766, 990, 1467, 2233, 2499
AjuI GAANNNNNNNTTGG 4 cut(s) 630, 662, 1955, 1987
AluBI AGCT 4 cut(s) 98, 634, 1957, 2374
AluI AGCT 4 cut(s) 98, 634, 1957, 2374
Alw21I GWGCWC 2 cut(s) 491, 2358
Alw26I GTCTC 8 cut(s) 759, 795, 979, 1151, 1295, 1415, 1981, 2715
AlwI GGATC 3 cut(s) 165, 609, 1729
AlwNI CAGNNNCTG 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 1373
AoxI GGCC 4 cut(s) 16, 958, 1465, 2612
ApeKI GCWGC 2 cut(s) 98, 2700
ApoI RAATTY 8 cut(s) 907, 976, 1105, 1216, 1868, 1963, 2387, 2492
Asp700I GAANNNNTTC 6 cut(s) 39, 309, 741, 835, 2180, 2217
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 4 cut(s) 206, 946, 2294, 2612
AsuC2I CCSGG 1 cut(s) 2616
AsuHPI GGTGA 4 cut(s) 1859, 1903, 2507, 2638
AvaI CYCGRG 1 cut(s) 1373
AvaII GGWCC 3 cut(s) 206, 946, 2294
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 3 cut(s) 962, 1919, 2183
Bbv12I GWGCWC 2 cut(s) 491, 2358
BbvI GCAGC 2 cut(s) 85, 2712
BccI CCATC 7 cut(s) 52, 1529, 2020, 2036, 2271, 2367, 2617
BciT130I CCWGG 6 cut(s) 210, 768, 992, 1469, 2235, 2501
BciVI GTATCC 1 cut(s) 1017
BcnI CCSGG 1 cut(s) 2616
BcoDI GTCTC 8 cut(s) 759, 795, 979, 1151, 1295, 1415, 1981, 2715
BfaI CTAG 5 cut(s) 20, 620, 635, 2027, 2538
BfmI CTRYAG 2 cut(s) 1492, 1641
BfrI CTTAAG 1 cut(s) 1012
BfuAI ACCTGC 1 cut(s) 546
BfuI GTATCC 1 cut(s) 1017
BglII AGATCT 3 cut(s) 671, 1016, 1198
BisI GCNGC 2 cut(s) 99, 2701
BlsI GCNGC 2 cut(s) 100, 2702
BmcAI AGTACT 1 cut(s) 2549
Bme1390I CCNGG 7 cut(s) 210, 768, 992, 1469, 2235, 2501, 2616
Bme18I GGWCC 3 cut(s) 206, 946, 2294
BmeT110I CYCGRG 1 cut(s) 1373
BmgT120I GGNCC 4 cut(s) 206, 946, 2294, 2612
BmiI GGNNCC 2 cut(s) 430, 2295
BmrFI CCNGG 7 cut(s) 210, 768, 992, 1469, 2235, 2501, 2616
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 7 cut(s) 259, 376, 1553, 1694, 2062, 2084, 2138
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 3 cut(s) 962, 1919, 2183
Bpu10I CCTNAGC 2 cut(s) 396, 482
BpuEI CTTGAG 4 cut(s) 1003, 1208, 1277, 1979
BpuMI CCSGG 1 cut(s) 2616
BsaBI GATNNNNATC 2 cut(s) 156, 600
BsaI GGTCTC 2 cut(s) 795, 1151
BsaJI CCNNGG 3 cut(s) 209, 844, 1240
Bsc4I CCNNNNNNNGG 4 cut(s) 647, 1799, 2365, 2753
Bse1I ACTGG 6 cut(s) 203, 1006, 2363, 2747, 2763, 2776
Bse3DI GCAATG 1 cut(s) 1077
Bse8I GATNNNNATC 2 cut(s) 156, 600
BseBI CCWGG 6 cut(s) 210, 768, 992, 1469, 2235, 2501
BseDI CCNNGG 3 cut(s) 209, 844, 1240
BseGI GGATG 8 cut(s) 88, 1214, 1568, 1709, 2153, 2359, 2389, 2712
BseJI GATNNNNATC 2 cut(s) 156, 600
BseLI CCNNNNNNNGG 4 cut(s) 647, 1799, 2365, 2753
BseMI GCAATG 1 cut(s) 1077
BseMII CTCAG 4 cut(s) 410, 1475, 1820, 2241
BseNI ACTGG 6 cut(s) 203, 1006, 2363, 2747, 2763, 2776
BseXI GCAGC 2 cut(s) 85, 2712
BseYI CCCAGC 1 cut(s) 732
BshFI GGCC 4 cut(s) 18, 960, 1467, 2614
BsiHKAI GWGCWC 2 cut(s) 491, 2358
BsiHKCI CYCGRG 1 cut(s) 1373
BsiSI CCGG 1 cut(s) 2615
BslFI GGGAC 2 cut(s) 330, 1619
BslI CCNNNNNNNGG 4 cut(s) 647, 1799, 2365, 2753
BsmAI GTCTC 8 cut(s) 759, 795, 979, 1151, 1295, 1415, 1981, 2715
BsmFI GGGAC 2 cut(s) 330, 1619
BsnI GGCC 4 cut(s) 18, 960, 1467, 2614
Bso31I GGTCTC 2 cut(s) 795, 1151
BsoBI CYCGRG 1 cut(s) 1373
Bsp1286I GDGCHC 2 cut(s) 491, 2358
Bsp143I GATC 9 cut(s) 157, 601, 671, 1016, 1045, 1198, 1666, 1721, 2512
BspACI CCGC 2 cut(s) 115, 1786
BspANI GGCC 4 cut(s) 18, 960, 1467, 2614
BspCNI CTCAG 4 cut(s) 409, 1476, 1821, 2242
BspLI GGNNCC 2 cut(s) 430, 2295
BspMAI CTGCAG 2 cut(s) 1496, 1645
BspMI ACCTGC 1 cut(s) 546
BspPI GGATC 3 cut(s) 165, 609, 1729
BspTI CTTAAG 1 cut(s) 1012
BspTNI GGTCTC 2 cut(s) 795, 1151
BsrDI GCAATG 1 cut(s) 1077
BsrI ACTGG 6 cut(s) 203, 1006, 2363, 2747, 2763, 2776
BssECI CCNNGG 3 cut(s) 209, 844, 1240
BssMI GATC 9 cut(s) 157, 601, 671, 1016, 1045, 1198, 1666, 1721, 2512
BssNAI GTATAC 1 cut(s) 2024
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 2 cut(s) 844, 1240
Bst1107I GTATAC 1 cut(s) 2024
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 6 cut(s) 210, 768, 992, 1469, 2235, 2501
Bst4CI ACNGT 5 cut(s) 702, 753, 1610, 2576, 2582
Bst6I CTCTTC 1 cut(s) 812
BstAFI CTTAAG 1 cut(s) 1012
BstAPI GCANNNNNTGC 2 cut(s) 715, 2784
BstC8I GCNNGC 7 cut(s) 466, 577, 632, 1790, 2302, 2472, 2789
BstDEI CTNAG 8 cut(s) 396, 482, 1484, 1829, 1904, 1953, 2250, 2305
BstEII GGTNACC 1 cut(s) 1891
BstF5I GGATG 8 cut(s) 88, 1214, 1568, 1709, 2153, 2359, 2389, 2712
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 9 cut(s) 160, 604, 674, 1019, 1048, 1201, 1669, 1724, 2515
BstMAI GTCTC 8 cut(s) 759, 795, 979, 1151, 1295, 1415, 1981, 2715
BstMBI GATC 9 cut(s) 157, 601, 671, 1016, 1045, 1198, 1666, 1721, 2512
BstMWI GCNNNNNNNGC 4 cut(s) 715, 1954, 2333, 2784
BstNI CCWGG 6 cut(s) 210, 768, 992, 1469, 2235, 2501
BstNSI RCATGY 2 cut(s) 579, 2304
BstPI GGTNACC 1 cut(s) 1891
BstSCI CCNGG 7 cut(s) 208, 766, 990, 1467, 2233, 2499, 2614
BstSFI CTRYAG 2 cut(s) 1492, 1641
BstV1I GCAGC 2 cut(s) 85, 2712
BstV2I GAAGAC 3 cut(s) 962, 1919, 2183
BstX2I RGATCY 5 cut(s) 601, 671, 1016, 1198, 1721
BstYI RGATCY 5 cut(s) 601, 671, 1016, 1198, 1721
BstZ17I GTATAC 1 cut(s) 2024
BsuI GTATCC 1 cut(s) 1017
BsuRI GGCC 4 cut(s) 18, 960, 1467, 2614
BtsCI GGATG 8 cut(s) 88, 1214, 1568, 1709, 2153, 2359, 2389, 2712
BtsIMutI CAGTG 4 cut(s) 2054, 2370, 2769, 2770
BveI ACCTGC 1 cut(s) 546
Cac8I GCNNGC 7 cut(s) 466, 577, 632, 1790, 2302, 2472, 2789
CaiI CAGNNNCTG 1 cut(s) 98
CfoI GCGC 1 cut(s) 259
Cfr13I GGNCC 4 cut(s) 206, 946, 2294, 2612
Csp6I GTAC 5 cut(s) 1518, 2049, 2345, 2548, 2556
CviAII CATG 8 cut(s) 260, 576, 710, 794, 1664, 2185, 2301, 2351
CviQI GTAC 5 cut(s) 1518, 2049, 2345, 2548, 2556
DdeI CTNAG 8 cut(s) 396, 482, 1484, 1829, 1904, 1953, 2250, 2305
DpnI GATC 9 cut(s) 159, 603, 673, 1018, 1047, 1200, 1668, 1723, 2514
DpnII GATC 9 cut(s) 157, 601, 671, 1016, 1045, 1198, 1666, 1721, 2512
DraI TTTAAA 1 cut(s) 2034
Eam1104I CTCTTC 1 cut(s) 812
EarI CTCTTC 1 cut(s) 812
Eco130I CCWWGG 2 cut(s) 844, 1240
Eco147I AGGCCT 1 cut(s) 960
Eco31I GGTCTC 2 cut(s) 795, 1151
Eco47I GGWCC 3 cut(s) 206, 946, 2294
Eco57I CTGAAG 5 cut(s) 324, 836, 975, 2195, 2450
Eco88I CYCGRG 1 cut(s) 1373
Eco91I GGTNACC 1 cut(s) 1891
EcoO109I RGGNCCY 1 cut(s) 2294
EcoO65I GGTNACC 1 cut(s) 1891
EcoRI GAATTC 1 cut(s) 2387
EcoRII CCWGG 6 cut(s) 208, 766, 990, 1467, 2233, 2499
EcoT14I CCWWGG 2 cut(s) 844, 1240
EcoT22I ATGCAT 2 cut(s) 711, 2077
ErhI CCWWGG 2 cut(s) 844, 1240
FaeI CATG 8 cut(s) 263, 579, 713, 797, 1667, 2188, 2304, 2354
FalI AAGNNNNNCTT 2 cut(s) 24, 56
FaqI GGGAC 2 cut(s) 330, 1619
FatI CATG 8 cut(s) 259, 575, 709, 793, 1663, 2184, 2300, 2350
FauNDI CATATG 1 cut(s) 2223
FblI GTMKAC 1 cut(s) 2023
Fnu4HI GCNGC 2 cut(s) 99, 2701
FokI GGATG 8 cut(s) 95, 1221, 1575, 1716, 2160, 2346, 2396, 2719
Fsp4HI GCNGC 2 cut(s) 99, 2701
FspBI CTAG 5 cut(s) 20, 620, 635, 2027, 2538
GlaI GCGC 1 cut(s) 258
GluI GCNGC 2 cut(s) 99, 2701
GsaI CCCAGC 1 cut(s) 736
HaeIII GGCC 4 cut(s) 18, 960, 1467, 2614
HapII CCGG 1 cut(s) 2615
HhaI GCGC 1 cut(s) 259
Hin1II CATG 8 cut(s) 263, 579, 713, 797, 1667, 2188, 2304, 2354
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HincII GTYRAC 2 cut(s) 165, 864
HindII GTYRAC 2 cut(s) 165, 864
HindIII AAGCTT 2 cut(s) 1955, 2372
HpaII CCGG 1 cut(s) 2615
HphI GGTGA 4 cut(s) 1859, 1903, 2507, 2638
Hpy166II GTNNAC 5 cut(s) 165, 749, 864, 946, 2024
Hpy8I GTNNAC 5 cut(s) 165, 749, 864, 946, 2024
HpyAV CCTTC 8 cut(s) 322, 950, 959, 1199, 1752, 1844, 1941, 2018
HpyCH4III ACNGT 5 cut(s) 702, 753, 1610, 2576, 2582
HpyCH4IV ACGT 2 cut(s) 775, 1396
HpyF10VI GCNNNNNNNGC 4 cut(s) 715, 1954, 2333, 2784
HpyF3I CTNAG 8 cut(s) 396, 482, 1484, 1829, 1904, 1953, 2250, 2305
HpySE526I ACGT 2 cut(s) 775, 1396
Hsp92II CATG 8 cut(s) 263, 579, 713, 797, 1667, 2188, 2304, 2354
HspAI GCGC 1 cut(s) 257
Kzo9I GATC 9 cut(s) 157, 601, 671, 1016, 1045, 1198, 1666, 1721, 2512
LmnI GCTCC 3 cut(s) 95, 486, 1853
Lsp1109I GCAGC 2 cut(s) 85, 2712
LweI GCATC 7 cut(s) 259, 376, 1553, 1694, 2062, 2084, 2138
MaeI CTAG 5 cut(s) 20, 620, 635, 2027, 2538
MaeII ACGT 2 cut(s) 775, 1396
MaeIII GTNAC 6 cut(s) 607, 877, 1781, 1891, 2570, 2576
MalI GATC 9 cut(s) 159, 603, 673, 1018, 1047, 1200, 1668, 1723, 2514
MboI GATC 9 cut(s) 157, 601, 671, 1016, 1045, 1198, 1666, 1721, 2512
MfeI CAATTG 1 cut(s) 1449
MflI RGATCY 5 cut(s) 601, 671, 1016, 1198, 1721
MhlI GDGCHC 2 cut(s) 491, 2358
MlyI GAGTC 5 cut(s) 829, 874, 981, 1291, 2649
MmeI TCCRAC 2 cut(s) 714, 1363
Mph1103I ATGCAT 2 cut(s) 711, 2077
MroXI GAANNNNTTC 6 cut(s) 39, 309, 741, 835, 2180, 2217
MslI CAYNNNNRTG 2 cut(s) 854, 2268
MspCI CTTAAG 1 cut(s) 1012
MspI CCGG 1 cut(s) 2615
MspR9I CCNGG 7 cut(s) 210, 768, 992, 1469, 2235, 2501, 2616
MunI CAATTG 1 cut(s) 1449
MvaI CCWGG 6 cut(s) 210, 768, 992, 1469, 2235, 2501
MwoI GCNNNNNNNGC 4 cut(s) 715, 1954, 2333, 2784
NciI CCSGG 1 cut(s) 2616
NdeI CATATG 1 cut(s) 2223
NdeII GATC 9 cut(s) 157, 601, 671, 1016, 1045, 1198, 1666, 1721, 2512
NlaIII CATG 8 cut(s) 263, 579, 713, 797, 1667, 2188, 2304, 2354
NlaIV GGNNCC 2 cut(s) 430, 2295
NmuCI GTSAC 3 cut(s) 607, 877, 1891
NsiI ATGCAT 2 cut(s) 711, 2077
NspI RCATGY 2 cut(s) 579, 2304
PaeI GCATGC 2 cut(s) 579, 2304
PceI AGGCCT 1 cut(s) 960
PdmI GAANNNNTTC 6 cut(s) 39, 309, 741, 835, 2180, 2217
PflMI CCANNNNNTGG 1 cut(s) 2365
PfoI TCCNGGA 2 cut(s) 990, 2233
PkrI GCNGC 2 cut(s) 100, 2702
PleI GAGTC 5 cut(s) 828, 874, 981, 1291, 2648
PpsI GAGTC 5 cut(s) 828, 874, 981, 1291, 2648
PpuMI RGGWCCY 1 cut(s) 2294
Psp1406I AACGTT 1 cut(s) 1396
Psp5II RGGWCCY 1 cut(s) 2294
Psp6I CCWGG 6 cut(s) 208, 766, 990, 1467, 2233, 2499
PspEI GGTNACC 1 cut(s) 1891
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 6 cut(s) 208, 766, 990, 1467, 2233, 2499
PspN4I GGNNCC 2 cut(s) 430, 2295
PspPI GGNCC 4 cut(s) 206, 946, 2294, 2612
PspPPI RGGWCCY 1 cut(s) 2294
PstI CTGCAG 2 cut(s) 1496, 1645
PstNI CAGNNNCTG 1 cut(s) 98
PsuI RGATCY 5 cut(s) 601, 671, 1016, 1198, 1721
RsaI GTAC 5 cut(s) 1519, 2050, 2346, 2549, 2557
RsaNI GTAC 5 cut(s) 1518, 2049, 2345, 2548, 2556
RseI CAYNNNNRTG 2 cut(s) 854, 2268
SatI GCNGC 2 cut(s) 99, 2701
Sau3AI GATC 9 cut(s) 157, 601, 671, 1016, 1045, 1198, 1666, 1721, 2512
Sau96I GGNCC 4 cut(s) 206, 946, 2294, 2612
ScaI AGTACT 1 cut(s) 2549
SchI GAGTC 5 cut(s) 829, 874, 981, 1291, 2649
ScrFI CCNGG 7 cut(s) 210, 768, 992, 1469, 2235, 2501, 2616
SduI GDGCHC 2 cut(s) 491, 2358
SfaNI GCATC 7 cut(s) 259, 376, 1553, 1694, 2062, 2084, 2138
SfcI CTRYAG 2 cut(s) 1492, 1641
SinI GGWCC 3 cut(s) 206, 946, 2294
SmiMI CAYNNNNRTG 2 cut(s) 854, 2268
SmlI CTYRAG 5 cut(s) 982, 1012, 1187, 1292, 1958
SmoI CTYRAG 5 cut(s) 982, 1012, 1187, 1292, 1958
SphI GCATGC 2 cut(s) 579, 2304
SseBI AGGCCT 1 cut(s) 960
SsiI CCGC 2 cut(s) 115, 1786
SspI AATATT 3 cut(s) 561, 626, 2245
SspMI CTAG 5 cut(s) 20, 620, 635, 2027, 2538
StuI AGGCCT 1 cut(s) 960
StyD4I CCNGG 7 cut(s) 208, 766, 990, 1467, 2233, 2499, 2614
StyI CCWWGG 2 cut(s) 844, 1240
TaaI ACNGT 5 cut(s) 702, 753, 1610, 2576, 2582
TaiI ACGT 2 cut(s) 778, 1399
TatI WGTACW 3 cut(s) 2048, 2344, 2547
TscAI CASTG 4 cut(s) 2061, 2370, 2770, 2776
TseFI GTSAC 3 cut(s) 607, 877, 1891
TseI GCWGC 2 cut(s) 98, 2700
Tsp45I GTSAC 3 cut(s) 607, 877, 1891
TspDTI ATGAA 9 cut(s) 99, 1114, 2099, 2104, 2173, 2210, 2271, 2325, 2400
TspGWI ACGGA 3 cut(s) 396, 2103, 2633
TspRI CASTG 4 cut(s) 2061, 2370, 2770, 2776
Van91I CCANNNNNTGG 1 cut(s) 2365
Vha464I CTTAAG 1 cut(s) 1012
VpaK11BI GGWCC 3 cut(s) 206, 946, 2294
XapI RAATTY 8 cut(s) 907, 976, 1105, 1216, 1868, 1963, 2387, 2492
XceI RCATGY 2 cut(s) 579, 2304
XcmI CCANNNNNNNNNTGG 3 cut(s) 55, 1238, 2270
XmiI GTMKAC 1 cut(s) 2023
XmnI GAANNNNTTC 6 cut(s) 39, 309, 741, 835, 2180, 2217
XspI CTAG 5 cut(s) 20, 620, 635, 2027, 2538
ZrmI AGTACT 1 cut(s) 2549
Zsp2I ATGCAT 2 cut(s) 711, 2077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.