Rw6G000750

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000105
Physical Location & Seq
Forward (+)
1183074 .. 1206038
22965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000750.1

Sequence Viewer

Length: 4056 bp
ATGTCGTCAACACCCTCTTCTTCTTCTACTCCTCCTCGTCAATGGAAGTATGATGTTTTTCTTAGTTTCAGAGGAGAAGACACCCGCTTGAATTTTGTGGACCATCTGTATGCTGCATTAGGTCAGAAAGGAATTGTCACCTTTAGAGATGACAAAAGTCTTGAGAGAGGAAAATCCATTTCGTCAGAACTTCTTAAAGCAATTGAGGAGTCAAAATTTGCTCTTGTCATTCTTTCACCAAACTATGCTTCTTCTTCATGGTGCTTAGACGAGCTTTTGAAGATTATTCAATGCAGAGAAGAAATGGGGCAACAAGTCATGCCAGTGTTCTACCAAGTAGATCCATCTCATGTGCGTAAGAACAAGGGATATTTTTTGCAGGCCTTTACAGAACACGAAGAAGTTCATTGGGAGAACAGGGATAAAGTAAGGAAGTGGAGGGCTGCTTTGACAGGCGTGGCCAATCTTTCTGGGTGGGATTTGAGGAACATGCATGAGGCAAAAGCTATCAAGAGTATTGTTAAAGATATACTAAATAAATCACAGTATTCATCTACAAGCGTTTACAAGGGCATGGTTGGAATGGATGTCCGTGTTGATGATTTACTGAGAAATCATATTCGTCCACATTTTGATGATGTGCGCATGATAGGGATCCATGGAATGCGGGGAATAGGTAAGACGGCAATTGCCCGAGCTCTTTATGATCAACTTGGTCAAGATTTTGACAGTAGCTGCGAGCCTTCCAATGTTAGAGAAGTGTTTAGCAAAGGTGGTATATATCCTTCACAGTATGAAATTAGTGACTGTTTTGACCATTGCTGCTTTCTTTCCAACGTTAGAGAAATGTCAAAAAAAGATGGTCTAGTTTCTCTTCAAGAAAAACTTCTTTCCAGAGTCTTGAGGGCAACAATTAGGAACATAGAGGATGAATATGCTGGAGCGAATATGATACAAAGACGATTAGGTAAATTAAGGGTGCTGATTGTTATTGATGATGTGGATCAAGTGGCACAATTAGAATGTTTGGCTGGAAATCATGACTGGTTCGGTCCGGGAAGTAGAATTATCATAACCAGTGCTGATGTTCATTTGTTGAATTGTGTGGATACTCTATACAAGGCTGCTGTGTTAACCCATGATGAAGCTCTTCAACTTTTGAGTTTAAAGGCTTTTAAGAAAAACCCCCCGCCTGAAGATTACTTGGAGCTATGCAACAATATTTTAGGGTATGCTCATGGGCTTCCATTAGTTCTTGTGGTTATAGGTCCATTTCTATGTGGTAGATCGATCAATGAATGGAAGAGTGCAATCGATAGGCTCAGGAATAAACCAGAAACTAGCATCGTTGATGTGCTTCAGATAAGTTTTGATGGGCTAAGGGAAACAGAAAAAGAAATTTTCCTACATGTCGCTTGCTTTTATGTGGGGAAGGATAGGGATCGTGTATCAGAAATACTAGATTATTGTCAGCTTTACCCTGACATTGGTTTAAGTGTTCTTGCTGATAAATCTCTCATTACTATTTCCAACAACAAACTATCCATGCATGCTTTGCTACAAGAAATGGGCTGGGAGATAGTTCGTAGGGAGTCACCTAAACCAGGCAGGCGTACTAGATTATGGTCTCATGAAGACATCCACAATGTGCTGAAAAAAAATAAGGGAACAGAAGCAGTTCAAGGCATGGTAATGGACTTGCCTAAATTAGAAGTGGCTAATTGGAACCCCGAAGCATTCTCAAAGTTGTCTGAGCTTAACTTTCTCCAAATCCGTAATGTCGACCTTCCCAATGGCCTCACTTGCCTCTCTAATTCCTTGAGACTCCTCGAATGGAGTGAGTATCCCTTAAGATCTCTGCCACAAAATTTCGAAGCAGATGAACTTATTGAACTTAATTTGTGCCACAGCAAAATTGAACATCTTTGGGAGGGAACAAAGTGTTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAGAACATTGTCCAGACCCCAGACCTCACAGGCGCTTGTAGTCTTGAGTGTCTAGATCTTGAAGGGTGTAAAAACTTGGTAAAAATCCATCAATCCGTTGGAGTTCTCAAAAAGCTTATTGTCTTGAATCTTAAAGACTGTGAGAGTCTGCAGAGTCTACCAGATAGAATTGAAATGGAGTCTCTTAAAACTCTTATTCTTTCTGGGTGTTCCAATATCAAGAAGATTCCTGAGTTTAGCAGAAATATGGAGTGTTTGTCAGAGCTGTGTCTTGATAGGACTGCCATTGAAAAACTGCCCGACTCAATCCAGTACCTATGTGGCCTGTCTTTCTTGGATCTAAGCAACTGCAAGAATCTTTTTTGTCTCCCAAGCACCATTAATAGATTGACAAGTCTTAAAAATATTAATCTTTCAGGATGCCAGAAGCTCGGGGGACCGGATAAAAGTAATTTTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCAGACCCCAGACCTCACAGGCGTTCAAAATCTTGAGACTCTAGATCTTGAAGGATGTAAGACTTTGGTTGAACTTCATCAATCCCTTGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAAGACTGCGAGAGTCTCGAGAGTCTGCCAAGTAAAATTGAAACGGAATCTCTTGAAACTTTAATTCTTTCTAACTGCTCAAAAGTTAAAAAGATTCCCCAGTTCGCTGGAAATATGGGGCGCTTATCAAAGCTTTATCTCGATGAGACAGCCATTGAAACACTACCTGTGTCAATTGGATGTCTGAGTGGCCTTGCTTCACTGAATCTGAGCAACTGCAAAAATCTTGTTTGCCTTCCAAGCACCATTAGTAGATTGGAGTCTCTTCAAAATCTTGATCTTTCTGGATGTTTGAAACTTGGCAAACAGGATAGTGCGAGGGAGAATGGTGCAATTAGAACTGCTGTAAATCATCTTACAACGTGCTTTTGTGAAGTTGTTGGGACATATTTGTCATGGTCCTGGCCTTCTAGATTGGTGCAAAGAGCCGATATAGGACCAATGAGTGTACAGTTGCCTGTGTCTGATCTGTGTTATTCTACAGAGCAAAATGCAAATACATCAATGAGTATCTGGTTCCCACCATCTCGTCTATGTAATTTAACAGATCTAAACATAAGTAACTGCAATCTTGGTGATGCTGCGTTTGCCAACTTTGCCTGTTTTCCCTCCTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTATGCTTCAGAACATTAATTTGGAGAATTGCAAGACACTTCAAGAGTTGTCTGGACTTCCATCAAATAGAAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAGATGCTGTTTGATGTATCCAATTTCAATAGTTTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGAAAATCAGGGCTGCAGTAACATAGCATTTGAAATGCTACGGATATTCCTTGATCAGCAAATTTCTAGTGCGACGGAAACTTTTCAAATTGTAATTCCTGGAAATAGAATTCCTGAGTGGTTTGATCATCAAAGATTTGGGAGTTCATTAACTGCAGACCTACTTCCAGGTTGGAATAAGAGTAGGTTTTTGGGATTTGTTTTGTGTGCTGTTTTTGTACTCCATGAGCACCGTCAGGTTGATGAGCTTGATGTCCTGACGTTCAAGACTTTTAAGGCTACTCATCATCTTGTATGTTGCCTGAAGCTGAATGGAAAAGAATTAGAAGTACATGGCAAACAGCCTGCATTTCGCTTTAGCGAACAGTTTTGCCAGGTTGAGTCAGATCACCTTTGGATATTCTTTGTGTCCCGTGATAAATACTTTGGTACAGATTGGTGGCATAACAGTTGCCGTCAGCTTGAGTTCTTATTTGAAACAAGAGGGCCAGGTCTGGAGGTGAAGGAGTGTGGGGTACGTCTGATATATGAGCAAGATGTGCTAGAGCTGAACCAAACAATAACTCAATCAAGCAGTAGGATGTCTTCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1351

Amino Acids

152.73

Weight (kDa)

6.29

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 193 9.3e-58 TIR domain
TIR_2 PF13676 19 - 116 3.5e-17 TIR domain
NB-ARC PF00931 263 - 395 1.5e-08 NB-ARC domain
WHD_ROQ1 PF23282 462 - 532 6.3e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 629 - 647 4.8e-06 Leucine Rich Repeat
LRR_13 PF23286 720 - 804 5.1e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 823 - 1084 1.6e-12 Leucine-rich repeat region
LRR_13 PF23286 884 - 968 7.9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1186 - 1334 4.7e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2992
Acc16I TGCGCA 1 cut(s) 644
AccI GTMKAC 2 cut(s) 1782, 2137
AciI CCGC 3 cut(s) 85, 667, 1190
AclI AACGTT 1 cut(s) 837
AclWI GGATC 6 cut(s) 335, 649, 662, 1011, 1449, 2325
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 6 cut(s) 91, 215, 1398, 1867, 3522, 3570
AcuI CTGAAG 4 cut(s) 1215, 1343, 3269, 3785
AdeI CACNNNGTG 1 cut(s) 1648
AfaI GTAC 7 cut(s) 1615, 2294, 3051, 3681, 3792, 3892, 3978
AfiI CCNNNNNNNGG 2 cut(s) 1487, 1604
AflII CTTAAG 1 cut(s) 1849
AflIII ACRYGT 1 cut(s) 1408
AjnI CCWGG 6 cut(s) 1603, 3002, 3559, 3628, 3834, 3949
AjuI GAANNNNNNNTTGG 4 cut(s) 232, 264, 3281, 3313
Alw21I GWGCWC 2 cut(s) 700, 3693
Alw26I GTCTC 8 cut(s) 1632, 1816, 2166, 2351, 2510, 2621, 2741, 2868
AlwI GGATC 6 cut(s) 335, 649, 662, 1011, 1449, 2325
AlwNI CAGNNNCTG 2 cut(s) 735, 3273
Ama87I CYCGRG 3 cut(s) 693, 2411, 2618
AoxI GGCC 7 cut(s) 381, 459, 1795, 2302, 2791, 3005, 3947
ApeKI GCWGC 7 cut(s) 113, 443, 735, 822, 1124, 3182, 3474
ApoI RAATTY 6 cut(s) 91, 215, 1398, 1867, 3522, 3570
AseI ATTAAT 3 cut(s) 2361, 2388, 3294
Asp700I GAANNNNTTC 5 cut(s) 402, 885, 1149, 1677, 3543
AspLEI GCGC 3 cut(s) 645, 2015, 2724
AspS9I GGNCC 8 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270, 3947
AsuC2I CCSGG 1 cut(s) 1056
AsuHPI GGTGA 6 cut(s) 130, 228, 1587, 3188, 3842, 3973
AsuII TTCGAA 1 cut(s) 1872
AvaI CYCGRG 3 cut(s) 693, 2411, 2618
AvaII GGWCC 7 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 1 cut(s) 654
BanII GRGCYC 1 cut(s) 700
BarI GAAGNNNNNNTAC 3 cut(s) 769, 801, 4030
BbsI GAAGAC 3 cut(s) 84, 1641, 4038
Bbv12I GWGCWC 2 cut(s) 700, 3693
BbvI GCAGC 7 cut(s) 100, 430, 722, 809, 1111, 3169, 3461
BccI CCATC 8 cut(s) 111, 352, 854, 1367, 2076, 3133, 3346, 3362
BceAI ACGGC 2 cut(s) 699, 3900
BciT130I CCWGG 6 cut(s) 1605, 3004, 3561, 3630, 3836, 3951
BciVI GTATCC 3 cut(s) 1102, 1854, 3412
BclI TGATCA 3 cut(s) 706, 3514, 3586
BcnI CCSGG 1 cut(s) 1056
BcoDI GTCTC 8 cut(s) 1632, 1816, 2166, 2351, 2510, 2621, 2741, 2868
BfaI CTAG 9 cut(s) 866, 1341, 1460, 1617, 2033, 2522, 3012, 3528, 4004
BfmI CTRYAG 4 cut(s) 2129, 3081, 3475, 3615
BfoI RGCGCY 2 cut(s) 2016, 2725
BfrI CTTAAG 1 cut(s) 1849
BfuI GTATCC 3 cut(s) 1102, 1854, 3412
BglII AGATCT 4 cut(s) 1853, 2035, 2524, 3148
BisI GCNGC 7 cut(s) 114, 444, 736, 823, 1125, 3183, 3475
BlsI GCNGC 7 cut(s) 115, 445, 737, 824, 1126, 3184, 3476
Bme1390I CCNGG 7 cut(s) 1056, 1605, 3004, 3561, 3630, 3836, 3951
Bme18I GGWCC 7 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270
BmeT110I CYCGRG 3 cut(s) 693, 2411, 2618
BmgT120I GGNCC 8 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270, 3947
BmiI GGNNCC 4 cut(s) 656, 1727, 2418, 3119
BmrFI CCNGG 7 cut(s) 1056, 1605, 3004, 3561, 3630, 3836, 3951
BmrI ACTGGG 1 cut(s) 2695
BmsI GCATC 5 cut(s) 1353, 2390, 3169, 3273, 3378
BmuI ACTGGG 1 cut(s) 2695
BoxI GACNNNNGTC 1 cut(s) 156
BpiI GAAGAC 3 cut(s) 84, 1641, 4038
BpmI CTGGAG 3 cut(s) 960, 3404, 3977
Bpu10I CCTNAGC 2 cut(s) 1322, 1379
Bpu14I TTCGAA 1 cut(s) 1872
BpuEI CTTGAG 6 cut(s) 182, 922, 1840, 2045, 2534, 3944
BpuMI CCSGG 1 cut(s) 1056
Bsa29I ATCGAT 2 cut(s) 1289, 1314
BsaBI GATNNNNATC 3 cut(s) 653, 1002, 1440
BsaI GGTCTC 1 cut(s) 1632
BsaJI CCNNGG 2 cut(s) 658, 2566
BsaWI WCCGGW 1 cut(s) 2419
BsaXI ACNNNNNCTCC 4 cut(s) 2222, 2252, 3377, 3407
Bsc4I CCNNNNNNNGG 2 cut(s) 1487, 1604
Bse1I ACTGG 6 cut(s) 323, 1049, 1077, 2290, 2701, 3387
Bse3DI GCAATG 1 cut(s) 817
Bse8I GATNNNNATC 3 cut(s) 653, 1002, 1440
BseBI CCWGG 6 cut(s) 1605, 3004, 3561, 3630, 3836, 3951
BseCI ATCGAT 2 cut(s) 1289, 1314
BseDI CCNNGG 2 cut(s) 658, 2566
BseGI GGATG 9 cut(s) 592, 934, 1638, 2405, 2540, 2786, 2894, 3361, 4047
BseJI GATNNNNATC 3 cut(s) 653, 1002, 1440
BseLI CCNNNNNNNGG 2 cut(s) 1487, 1604
BseMI GCAATG 1 cut(s) 817
BseMII CTCAG 9 cut(s) 599, 1336, 1743, 1994, 2202, 2777, 2801, 3221, 3567
BseNI ACTGG 6 cut(s) 323, 1049, 1077, 2290, 2701, 3387
BseRI GAGGAG 5 cut(s) 21, 24, 87, 221, 1817
BseXI GCAGC 7 cut(s) 100, 430, 722, 809, 1111, 3169, 3461
BseYI CCCAGC 1 cut(s) 1572
BshFI GGCC 7 cut(s) 383, 461, 1797, 2304, 2793, 3007, 3949
BshVI ATCGAT 2 cut(s) 1289, 1314
BsiHKAI GWGCWC 2 cut(s) 700, 3693
BsiHKCI CYCGRG 3 cut(s) 693, 2411, 2618
BsiSI CCGG 2 cut(s) 1055, 2420
BslFI GGGAC 3 cut(s) 2430, 2998, 3856
BslI CCNNNNNNNGG 2 cut(s) 1487, 1604
BsmAI GTCTC 8 cut(s) 1632, 1816, 2166, 2351, 2510, 2621, 2741, 2868
BsmFI GGGAC 3 cut(s) 2430, 2998, 3856
BsmI GAATGC 2 cut(s) 669, 1736
BsnI GGCC 7 cut(s) 383, 461, 1797, 2304, 2793, 3007, 3949
Bso31I GGTCTC 1 cut(s) 1632
BsoBI CYCGRG 3 cut(s) 693, 2411, 2618
Bsp119I TTCGAA 1 cut(s) 1872
Bsp1286I GDGCHC 2 cut(s) 700, 3693
Bsp1407I TGTACA 1 cut(s) 3049
Bsp19I CCATGG 1 cut(s) 658
BspACI CCGC 3 cut(s) 85, 667, 1190
BspANI GGCC 7 cut(s) 383, 461, 1797, 2304, 2793, 3007, 3949
BspCNI CTCAG 9 cut(s) 600, 1335, 1744, 1993, 2203, 2778, 2802, 3222, 3568
BspDI ATCGAT 2 cut(s) 1289, 1314
BspHI TCATGA 2 cut(s) 1039, 1630
BspLI GGNNCC 4 cut(s) 656, 1727, 2418, 3119
BspMAI CTGCAG 3 cut(s) 2133, 3479, 3619
BspPI GGATC 6 cut(s) 335, 649, 662, 1011, 1449, 2325
BspQI GCTCTTC 1 cut(s) 1155
BspT104I TTCGAA 1 cut(s) 1872
BspTI CTTAAG 1 cut(s) 1849
BspTNI GGTCTC 1 cut(s) 1632
BsrDI GCAATG 1 cut(s) 817
BsrGI TGTACA 1 cut(s) 3049
BsrI ACTGG 6 cut(s) 323, 1049, 1077, 2290, 2701, 3387
BssECI CCNNGG 2 cut(s) 658, 2566
BssT1I CCWWGG 2 cut(s) 658, 2566
Bst2UI CCWGG 6 cut(s) 1605, 3004, 3561, 3630, 3836, 3951
Bst4CI ACNGT 9 cut(s) 546, 731, 792, 809, 2120, 3054, 3695, 3828, 3911
Bst6I CTCTTC 5 cut(s) 22, 879, 1155, 1298, 2871
BstAFI CTTAAG 1 cut(s) 1849
BstAPI GCANNNNNTGC 2 cut(s) 1555, 4000
BstAUI TGTACA 1 cut(s) 3049
BstBI TTCGAA 1 cut(s) 1872
BstC8I GCNNGC 6 cut(s) 381, 740, 1417, 1551, 1610, 3807
BstDSI CCRYGG 1 cut(s) 658
BstENI CCTNNNNNAGG 1 cut(s) 1602
BstF5I GGATG 9 cut(s) 592, 934, 1638, 2405, 2540, 2786, 2894, 3361, 4047
BstH2I RGCGCY 2 cut(s) 2016, 2725
BstHHI GCGC 3 cut(s) 645, 2015, 2724
BstMAI GTCTC 8 cut(s) 1632, 1816, 2166, 2351, 2510, 2621, 2741, 2868
BstMWI GCNNNNNNNGC 6 cut(s) 1555, 1803, 2841, 3188, 3197, 4000
BstNI CCWGG 6 cut(s) 1605, 3004, 3561, 3630, 3836, 3951
BstNSI RCATGY 3 cut(s) 493, 1412, 1553
BstPAI GACNNNNGTC 1 cut(s) 156
BstSCI CCNGG 7 cut(s) 1054, 1603, 3002, 3559, 3628, 3834, 3949
BstSFI CTRYAG 4 cut(s) 2129, 3081, 3475, 3615
BstV1I GCAGC 7 cut(s) 100, 430, 722, 809, 1111, 3169, 3461
BstV2I GAAGAC 3 cut(s) 84, 1641, 4038
BstX2I RGATCY 7 cut(s) 340, 654, 1853, 2035, 2317, 2524, 3148
BstXI CCANNNNNNTGG 1 cut(s) 2708
BstYI RGATCY 7 cut(s) 340, 654, 1853, 2035, 2317, 2524, 3148
Bsu15I ATCGAT 2 cut(s) 1289, 1314
BsuI GTATCC 3 cut(s) 1102, 1854, 3412
BsuRI GGCC 7 cut(s) 383, 461, 1797, 2304, 2793, 3007, 3949
BsuTUI ATCGAT 2 cut(s) 1289, 1314
BtgI CCRYGG 1 cut(s) 658
BtsCI GGATG 9 cut(s) 592, 934, 1638, 2405, 2540, 2786, 2894, 3361, 4047
BtsIMutI CAGTG 4 cut(s) 330, 1084, 2801, 3380
Cac8I GCNNGC 6 cut(s) 381, 740, 1417, 1551, 1610, 3807
CaiI CAGNNNCTG 2 cut(s) 735, 3273
CciI TCATGA 2 cut(s) 1039, 1630
CfoI GCGC 3 cut(s) 645, 2015, 2724
Cfr13I GGNCC 8 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270, 3947
ClaI ATCGAT 2 cut(s) 1289, 1314
CpoI CGGWCCG 1 cut(s) 1052
Csp6I GTAC 7 cut(s) 1614, 2293, 3050, 3680, 3791, 3891, 3977
CspI CGGWCCG 1 cut(s) 1052
CviQI GTAC 7 cut(s) 1614, 2293, 3050, 3680, 3791, 3891, 3977
DraI TTTAAA 1 cut(s) 1167
DraIII CACNNNGTG 1 cut(s) 1648
DrdI GACNNNNNNGTC 1 cut(s) 2992
DseDI GACNNNNNNGTC 1 cut(s) 2992
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 5 cut(s) 22, 879, 1155, 1298, 2871
EarI CTCTTC 5 cut(s) 22, 879, 1155, 1298, 2871
Ecl136II GAGCTC 1 cut(s) 698
Eco130I CCWWGG 2 cut(s) 658, 2566
Eco147I AGGCCT 1 cut(s) 383
Eco24I GRGCYC 1 cut(s) 700
Eco31I GGTCTC 1 cut(s) 1632
Eco47I GGWCC 7 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270
Eco53kI GAGCTC 1 cut(s) 698
Eco57I CTGAAG 4 cut(s) 1215, 1343, 3269, 3785
Eco88I CYCGRG 3 cut(s) 693, 2411, 2618
EcoICRI GAGCTC 1 cut(s) 698
EcoNI CCTNNNNNAGG 1 cut(s) 1602
EcoO109I RGGNCCY 1 cut(s) 3270
EcoRI GAATTC 1 cut(s) 3570
EcoRII CCWGG 6 cut(s) 1603, 3002, 3559, 3628, 3834, 3949
EcoT14I CCWWGG 2 cut(s) 658, 2566
EcoT22I ATGCAT 2 cut(s) 495, 1551
EcoT38I GRGCYC 1 cut(s) 700
ErhI CCWWGG 2 cut(s) 658, 2566
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FaqI GGGAC 3 cut(s) 2430, 2998, 3856
FauI CCCGC 3 cut(s) 92, 660, 1197
FbaI TGATCA 3 cut(s) 706, 3514, 3586
FblI GTMKAC 2 cut(s) 1782, 2137
Fnu4HI GCNGC 7 cut(s) 114, 444, 736, 823, 1125, 3183, 3475
FokI GGATG 8 cut(s) 599, 941, 1625, 2412, 2547, 2793, 2901, 3368
FriOI GRGCYC 1 cut(s) 700
Fsp4HI GCNGC 7 cut(s) 114, 444, 736, 823, 1125, 3183, 3475
FspAI RTGCGCAY 1 cut(s) 644
FspBI CTAG 9 cut(s) 866, 1341, 1460, 1617, 2033, 2522, 3012, 3528, 4004
FspI TGCGCA 1 cut(s) 644
GlaI GCGC 3 cut(s) 644, 2014, 2723
GluI GCNGC 7 cut(s) 114, 444, 736, 823, 1125, 3183, 3475
GsaI CCCAGC 1 cut(s) 1576
GsuI CTGGAG 3 cut(s) 960, 3404, 3977
HaeII RGCGCY 2 cut(s) 2016, 2725
HaeIII GGCC 7 cut(s) 383, 461, 1797, 2304, 2793, 3007, 3949
HapII CCGG 2 cut(s) 1055, 2420
HhaI GCGC 3 cut(s) 645, 2015, 2724
Hin6I GCGC 3 cut(s) 643, 2013, 2722
HinP1I GCGC 3 cut(s) 643, 2013, 2722
HincII GTYRAC 3 cut(s) 9, 1134, 1783
HindII GTYRAC 3 cut(s) 9, 1134, 1783
HindIII AAGCTT 3 cut(s) 2093, 2732, 3251
HpaI GTTAAC 1 cut(s) 1134
HpaII CCGG 2 cut(s) 1055, 2420
HphI GGTGA 6 cut(s) 130, 228, 1587, 3188, 3842, 3973
Hpy166II GTNNAC 8 cut(s) 9, 100, 565, 626, 1134, 1783, 2138, 3050
Hpy8I GTNNAC 8 cut(s) 9, 100, 565, 626, 1134, 1783, 2138, 3050
Hpy99I CGWCG 1 cut(s) 3538
HpyAV CCTTC 9 cut(s) 753, 795, 1426, 1796, 2036, 2525, 2846, 3018, 3958
HpyCH4III ACNGT 9 cut(s) 546, 731, 792, 809, 2120, 3054, 3695, 3828, 3911
HpyCH4IV ACGT 4 cut(s) 837, 2963, 3722, 3979
HpyF10VI GCNNNNNNNGC 6 cut(s) 1555, 1803, 2841, 3188, 3197, 4000
HpySE526I ACGT 4 cut(s) 837, 2963, 3722, 3979
HspAI GCGC 3 cut(s) 643, 2013, 2722
Ksp22I TGATCA 3 cut(s) 706, 3514, 3586
KspAI GTTAAC 1 cut(s) 1134
LguI GCTCTTC 1 cut(s) 1155
LmnI GCTCC 2 cut(s) 941, 1207
Lsp1109I GCAGC 7 cut(s) 100, 430, 722, 809, 1111, 3169, 3461
LweI GCATC 5 cut(s) 1353, 2390, 3169, 3273, 3378
MaeI CTAG 9 cut(s) 866, 1341, 1460, 1617, 2033, 2522, 3012, 3528, 4004
MaeII ACGT 4 cut(s) 837, 2963, 3722, 3979
MaeIII GTNAC 5 cut(s) 136, 803, 1593, 3161, 3479
MfeI CAATTG 4 cut(s) 201, 687, 2775, 3445
MflI RGATCY 7 cut(s) 340, 654, 1853, 2035, 2317, 2524, 3148
MhlI GDGCHC 2 cut(s) 700, 3693
MlsI TGGCCA 1 cut(s) 461
MluNI TGGCCA 1 cut(s) 461
MmeI TCCRAC 5 cut(s) 559, 858, 1554, 2059, 3614
Mox20I TGGCCA 1 cut(s) 461
Mph1103I ATGCAT 2 cut(s) 495, 1551
MroXI GAANNNNTTC 5 cut(s) 402, 885, 1149, 1677, 3543
MscI TGGCCA 1 cut(s) 461
MslI CAYNNNNRTG 5 cut(s) 108, 323, 633, 1276, 1691
Msp20I TGGCCA 1 cut(s) 461
MspCI CTTAAG 1 cut(s) 1849
MspI CCGG 2 cut(s) 1055, 2420
MspR9I CCNGG 7 cut(s) 1056, 1605, 3004, 3561, 3630, 3836, 3951
MunI CAATTG 4 cut(s) 201, 687, 2775, 3445
Mva1269I GAATGC 2 cut(s) 669, 1736
MvaI CCWGG 6 cut(s) 1605, 3004, 3561, 3630, 3836, 3951
MwoI GCNNNNNNNGC 6 cut(s) 1555, 1803, 2841, 3188, 3197, 4000
NciI CCSGG 1 cut(s) 1056
NcoI CCATGG 1 cut(s) 658
NlaIV GGNNCC 4 cut(s) 656, 1727, 2418, 3119
NmuCI GTSAC 3 cut(s) 136, 803, 1593
NsbI TGCGCA 1 cut(s) 644
NsiI ATGCAT 2 cut(s) 495, 1551
NspI RCATGY 3 cut(s) 493, 1412, 1553
NspV TTCGAA 1 cut(s) 1872
PaeI GCATGC 1 cut(s) 1553
PaeR7I CTCGAG 1 cut(s) 2618
PagI TCATGA 2 cut(s) 1039, 1630
PceI AGGCCT 1 cut(s) 383
PciI ACATGT 1 cut(s) 1408
PciSI GCTCTTC 1 cut(s) 1155
PctI GAATGC 2 cut(s) 669, 1736
PdmI GAANNNNTTC 5 cut(s) 402, 885, 1149, 1677, 3543
PfeI GAWTC 7 cut(s) 2107, 2206, 2335, 2596, 2648, 2695, 2806
PfoI TCCNGGA 2 cut(s) 1054, 3559
PkrI GCNGC 7 cut(s) 115, 445, 737, 824, 1126, 3184, 3476
PpuMI RGGWCCY 1 cut(s) 3270
PscI ACATGT 1 cut(s) 1408
PshAI GACNNNNGTC 1 cut(s) 156
PshBI ATTAAT 3 cut(s) 2361, 2388, 3294
Psp124BI GAGCTC 1 cut(s) 700
Psp1406I AACGTT 1 cut(s) 837
Psp5II RGGWCCY 1 cut(s) 3270
Psp6I CCWGG 6 cut(s) 1603, 3002, 3559, 3628, 3834, 3949
PspFI CCCAGC 1 cut(s) 1572
PspGI CCWGG 6 cut(s) 1603, 3002, 3559, 3628, 3834, 3949
PspN4I GGNNCC 4 cut(s) 656, 1727, 2418, 3119
PspPI GGNCC 8 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270, 3947
PspPPI RGGWCCY 1 cut(s) 3270
PstI CTGCAG 3 cut(s) 2133, 3479, 3619
PstNI CAGNNNCTG 2 cut(s) 735, 3273
PsuI RGATCY 7 cut(s) 340, 654, 1853, 2035, 2317, 2524, 3148
RsaI GTAC 7 cut(s) 1615, 2294, 3051, 3681, 3792, 3892, 3978
RsaNI GTAC 7 cut(s) 1614, 2293, 3050, 3680, 3791, 3891, 3977
RseI CAYNNNNRTG 5 cut(s) 108, 323, 633, 1276, 1691
Rsr2I CGGWCCG 1 cut(s) 1052
RsrII CGGWCCG 1 cut(s) 1052
SacI GAGCTC 1 cut(s) 700
SalI GTCGAC 1 cut(s) 1781
SapI GCTCTTC 1 cut(s) 1155
SatI GCNGC 7 cut(s) 114, 444, 736, 823, 1125, 3183, 3475
Sau96I GGNCC 8 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270, 3947
ScrFI CCNGG 7 cut(s) 1056, 1605, 3004, 3561, 3630, 3836, 3951
SduI GDGCHC 2 cut(s) 700, 3693
SfaNI GCATC 5 cut(s) 1353, 2390, 3169, 3273, 3378
SfcI CTRYAG 4 cut(s) 2129, 3081, 3475, 3615
Sfr274I CTCGAG 1 cut(s) 2618
SfuI TTCGAA 1 cut(s) 1872
SinI GGWCC 7 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270
SlaI CTCGAG 1 cut(s) 2618
SmiMI CAYNNNNRTG 5 cut(s) 108, 323, 633, 1276, 1691
SmlI CTYRAG 8 cut(s) 161, 901, 1819, 1849, 2024, 2513, 2618, 3923
SmoI CTYRAG 8 cut(s) 161, 901, 1819, 1849, 2024, 2513, 2618, 3923
SphI GCATGC 1 cut(s) 1553
SseBI AGGCCT 1 cut(s) 383
SsiI CCGC 3 cut(s) 85, 667, 1190
SspI AATATT 2 cut(s) 1222, 2386
SspMI CTAG 9 cut(s) 866, 1341, 1460, 1617, 2033, 2522, 3012, 3528, 4004
SstI GAGCTC 1 cut(s) 700
StuI AGGCCT 1 cut(s) 383
StyD4I CCNGG 7 cut(s) 1054, 1603, 3002, 3559, 3628, 3834, 3949
StyI CCWWGG 2 cut(s) 658, 2566
TaaI ACNGT 9 cut(s) 546, 731, 792, 809, 2120, 3054, 3695, 3828, 3911
TaiI ACGT 4 cut(s) 840, 2966, 3725, 3982
TaqI TCGA 7 cut(s) 1289, 1314, 1782, 1830, 1872, 2619, 2742
TaqII GACCGA 1 cut(s) 1040
TatI WGTACW 3 cut(s) 3049, 3679, 3790
TfiI GAWTC 7 cut(s) 2107, 2206, 2335, 2596, 2648, 2695, 2806
TscAI CASTG 4 cut(s) 330, 1084, 2808, 3387
TseFI GTSAC 3 cut(s) 136, 803, 1593
TseI GCWGC 7 cut(s) 113, 443, 735, 822, 1124, 3182, 3474
Tsp45I GTSAC 3 cut(s) 136, 803, 1593
TspGWI ACGGA 6 cut(s) 581, 1763, 2065, 2660, 3517, 3551
TspRI CASTG 4 cut(s) 330, 1084, 2808, 3387
Vha464I CTTAAG 1 cut(s) 1849
VpaK11BI GGWCC 7 cut(s) 100, 1052, 1268, 2417, 3000, 3038, 3270
VspI ATTAAT 3 cut(s) 2361, 2388, 3294
XagI CCTNNNNNAGG 1 cut(s) 1602
XapI RAATTY 6 cut(s) 91, 215, 1398, 1867, 3522, 3570
XbaI TCTAGA 3 cut(s) 2032, 2521, 3011
XceI RCATGY 3 cut(s) 493, 1412, 1553
XcmI CCANNNNNNNNNTGG 3 cut(s) 2075, 2297, 2854
XhoI CTCGAG 1 cut(s) 2618
XmiI GTMKAC 2 cut(s) 1782, 2137
XmnI GAANNNNTTC 5 cut(s) 402, 885, 1149, 1677, 3543
XspI CTAG 9 cut(s) 866, 1341, 1460, 1617, 2033, 2522, 3012, 3528, 4004
Zsp2I ATGCAT 2 cut(s) 495, 1551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.