RchiOBHm_Chr1g0319441

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
7020726 .. 7023292
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54968

Sequence Viewer

Length: 1986 bp
ATGCCGAAGGAGATGACTAGAAAGTGGAAATACGATGTCTTTCTAAGTTTCAGGGGGGAAGATACCCGCTATACTTTTACCAATCGTCTGAATTCTGCACTAATTCAAAAAGGGATTCTGACCTTAATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGACTCTAGATCTGCGATTGTCATTCTCTCAAAAAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGGAAGATATGGGCCAACAAGTCTTCCCCGTCTTCTACTTGGGAGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGTTTTGAGCTCATCAAATGGGAACACCAAGTAGATGTGGAAGTAAGGGAACATGAAGAGGTTTATGGGAAGAATGATGATAGACTAAATGCGTGGAGAGCTGCTTTGACTAAAGTGGCCAGTCTGTGTGGCTGGGATAACAAGAATTATGGATATGAACCATCACTAATTGAGGAAATTGTTAATCATATACTAAAGAATTCCGTGTATACATCTTCAAGTGTTCACAAGGGCTTCATAGGAATGGGTTCCCGCATTGATGATTTATTAAGCAATTACATATATCCGCATCTTGGTGGGGTGCGCTTTATAGGGATCCATGGCATGCGAGGCATAGGTAAGACAACACTTGCTCGAGCTATCTATGATGAAATCCGTCAGGATTTTTACCGGACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTTGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACTCAATTAGAAAAATTGGCTGGAAGTCACAACTGGTTTGGTCCGGGGAGTAGAATTGTCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTATGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATTCCCCCCACCAGAAGATTATTTGCATCTGTGCCACCATATTATAGGGTATGCTAAGGGGCTTCCCTTGGCTCTCGTGGTCTTAGGTTCTTTTCTATTTGGTAGAAGAACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGATGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAAAAATATGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGCGGCTCATTGGAAGCCGGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTTGTAATGTGGAACTTCCTGACGGCCTCACTTGTCTTTCTAATTCCCTGAGAATCGTCGAATGGACTGGGTATCCCTTGAGATCTCTCCCACAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGGTATGGCTATTAGTTAATAGATTTCTTGTTTATCTCTATGAAGCAAGTTGTCTGTTCTGTGTGGTGGCTCACTGTTTCAATATTATGAGCAGAATTTTGACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

661

Amino Acids

75.77

Weight (kDa)

6.13

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 10 - 195 3.4e-46 TIR domain
TIR_2 PF13676 13 - 107 1.8e-14 TIR domain
NB-ARC PF00931 218 - 372 3.3e-18 NB-ARC domain
NACHT PF05729 222 - 340 1.6e-07 NACHT domain
WHD_ROQ1 PF23282 439 - 510 1.3e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 608 - 626 1.7e-06 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 758
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 1 cut(s) 1624
AccI GTMKAC 1 cut(s) 563
AciI CCGC 6 cut(s) 67, 340, 607, 641, 889, 1652
AclWI GGATC 6 cut(s) 320, 333, 664, 677, 939, 1383
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 6 cut(s) 91, 553, 1115, 1681, 1804, 1971
AcuI CTGAAG 1 cut(s) 1610
AfiI CCNNNNNNNGG 3 cut(s) 647, 1421, 1624
AjnI CCWGG 1 cut(s) 1540
AluBI AGCT 8 cut(s) 142, 179, 364, 455, 713, 872, 1408, 1861
AluI AGCT 8 cut(s) 142, 179, 364, 455, 713, 872, 1408, 1861
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 3 cut(s) 1533, 1569, 1692
AlwI GGATC 6 cut(s) 320, 333, 664, 677, 939, 1383
AlwNI CAGNNNCTG 1 cut(s) 872
Ama87I CYCGRG 1 cut(s) 708
AoxI GGCC 5 cut(s) 164, 289, 471, 790, 1732
ApeKI GCWGC 3 cut(s) 179, 455, 872
ApoI RAATTY 6 cut(s) 91, 553, 1115, 1681, 1804, 1971
Asp700I GAANNNNTTC 5 cut(s) 601, 813, 1083, 1515, 1609
AspLEI GCGC 1 cut(s) 660
AspS9I GGNCC 3 cut(s) 289, 747, 980
AsuC2I CCSGG 1 cut(s) 984
AvaI CYCGRG 1 cut(s) 708
AvaII GGWCC 2 cut(s) 747, 980
BalI TGGCCA 1 cut(s) 473
BamHI GGATCC 2 cut(s) 325, 669
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1190
BbsI GAAGAC 4 cut(s) 292, 301, 1578, 1691
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 166, 442, 859
BccI CCATC 3 cut(s) 523, 826, 1301
BceAI ACGGC 1 cut(s) 1747
BciT130I CCWGG 1 cut(s) 1542
BciVI GTATCC 1 cut(s) 1791
BcnI CCSGG 1 cut(s) 984
BcoDI GTCTC 3 cut(s) 1533, 1569, 1692
BfaI CTAG 3 cut(s) 18, 195, 1394
BfuAI ACCTGC 1 cut(s) 758
BfuI GTATCC 1 cut(s) 1791
BglII AGATCT 3 cut(s) 197, 1445, 1790
BisI GCNGC 5 cut(s) 180, 341, 456, 873, 1653
BlsI GCNGC 5 cut(s) 181, 342, 457, 874, 1654
Bme1390I CCNGG 2 cut(s) 984, 1542
Bme18I GGWCC 2 cut(s) 747, 980
BmeT110I CYCGRG 1 cut(s) 708
BmgT120I GGNCC 3 cut(s) 289, 747, 980
BmiI GGNNCC 4 cut(s) 327, 344, 604, 671
BmrFI CCNGG 2 cut(s) 984, 1542
BmrI ACTGGG 1 cut(s) 1785
BmsI GCATC 4 cut(s) 652, 1033, 1150, 1470
BmuI ACTGGG 1 cut(s) 1785
BpiI GAAGAC 4 cut(s) 292, 301, 1578, 1691
Bpu10I CCTNAGC 1 cut(s) 1170
BpuEI CTTGAG 2 cut(s) 164, 1807
BpuMI CCSGG 1 cut(s) 984
BsaBI GATNNNNATC 2 cut(s) 930, 1374
BsaI GGTCTC 1 cut(s) 1569
BsaJI CCNNGG 4 cut(s) 673, 983, 1182, 1618
BsaWI WCCGGW 1 cut(s) 744
Bsc4I CCNNNNNNNGG 3 cut(s) 647, 1421, 1624
Bse1I ACTGG 4 cut(s) 474, 977, 1317, 1780
Bse8I GATNNNNATC 2 cut(s) 930, 1374
BseBI CCWGG 1 cut(s) 1542
BseDI CCNNGG 4 cut(s) 673, 983, 1182, 1618
BseGI GGATG 5 cut(s) 136, 256, 862, 1321, 1485
BseJI GATNNNNATC 2 cut(s) 930, 1374
BseLI CCNNNNNNNGG 3 cut(s) 647, 1421, 1624
BseMII CTCAG 1 cut(s) 1748
BseNI ACTGG 4 cut(s) 474, 977, 1317, 1780
BseXI GCAGC 3 cut(s) 166, 442, 859
BseYI CCCAGC 2 cut(s) 486, 1506
BsgI GTGCAG 1 cut(s) 81
BshFI GGCC 5 cut(s) 166, 291, 473, 792, 1734
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 708
BsiSI CCGG 3 cut(s) 745, 983, 1667
BslI CCNNNNNNNGG 3 cut(s) 647, 1421, 1624
BsmAI GTCTC 3 cut(s) 1533, 1569, 1692
BsnI GGCC 5 cut(s) 166, 291, 473, 792, 1734
Bso31I GGTCTC 1 cut(s) 1569
BsoBI CYCGRG 1 cut(s) 708
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 7 cut(s) 197, 325, 669, 931, 1375, 1445, 1790
Bsp19I CCATGG 1 cut(s) 673
BspACI CCGC 6 cut(s) 67, 340, 607, 641, 889, 1652
BspANI GGCC 5 cut(s) 166, 291, 473, 792, 1734
BspCNI CTCAG 1 cut(s) 1749
BspHI TCATGA 1 cut(s) 1567
BspLI GGNNCC 4 cut(s) 327, 344, 604, 671
BspMI ACCTGC 1 cut(s) 758
BspPI GGATC 6 cut(s) 320, 333, 664, 677, 939, 1383
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 1 cut(s) 1569
BsrI ACTGG 4 cut(s) 474, 977, 1317, 1780
BssECI CCNNGG 4 cut(s) 673, 983, 1182, 1618
BssMI GATC 7 cut(s) 197, 325, 669, 931, 1375, 1445, 1790
BssNAI GTATAC 1 cut(s) 564
BssSI CACGAG 1 cut(s) 1190
BssT1I CCWWGG 3 cut(s) 673, 1182, 1618
Bst1107I GTATAC 1 cut(s) 564
Bst2BI CACGAG 1 cut(s) 1190
Bst2UI CCWGG 1 cut(s) 1542
Bst4CI ACNGT 3 cut(s) 1476, 1527, 1952
Bst6I CTCTTC 1 cut(s) 405
BstAPI GCANNNNNTGC 1 cut(s) 1489
BstC8I GCNNGC 3 cut(s) 680, 1351, 1406
BstDEI CTNAG 5 cut(s) 44, 1170, 1198, 1694, 1757
BstDSI CCRYGG 1 cut(s) 673
BstF5I GGATG 5 cut(s) 136, 256, 862, 1321, 1485
BstHHI GCGC 1 cut(s) 660
BstKTI GATC 7 cut(s) 200, 328, 672, 934, 1378, 1448, 1793
BstMAI GTCTC 3 cut(s) 1533, 1569, 1692
BstMBI GATC 7 cut(s) 197, 325, 669, 931, 1375, 1445, 1790
BstMWI GCNNNNNNNGC 3 cut(s) 452, 684, 1489
BstNI CCWGG 1 cut(s) 1542
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 2 cut(s) 982, 1540
BstV1I GCAGC 3 cut(s) 166, 442, 859
BstV2I GAAGAC 4 cut(s) 292, 301, 1578, 1691
BstX2I RGATCY 5 cut(s) 197, 325, 669, 1445, 1790
BstYI RGATCY 5 cut(s) 197, 325, 669, 1445, 1790
BstZ17I GTATAC 1 cut(s) 564
BsuI GTATCC 1 cut(s) 1791
BsuRI GGCC 5 cut(s) 166, 291, 473, 792, 1734
BtgI CCRYGG 1 cut(s) 673
BtsCI GGATG 5 cut(s) 136, 256, 862, 1321, 1485
BtsIMutI CAGTG 1 cut(s) 1948
BveI ACCTGC 1 cut(s) 758
Cac8I GCNNGC 3 cut(s) 680, 1351, 1406
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 1 cut(s) 1567
CfoI GCGC 1 cut(s) 660
Cfr13I GGNCC 3 cut(s) 289, 747, 980
CseI GACGC 1 cut(s) 143
CviAII CATG 9 cut(s) 240, 277, 407, 674, 679, 1034, 1484, 1568, 1624
DdeI CTNAG 5 cut(s) 44, 1170, 1198, 1694, 1757
DpnI GATC 7 cut(s) 199, 327, 671, 933, 1377, 1447, 1792
DpnII GATC 7 cut(s) 197, 325, 669, 931, 1375, 1445, 1790
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 673, 1182, 1618
Eco147I AGGCCT 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 1569
Eco47I GGWCC 2 cut(s) 747, 980
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 1610
Eco88I CYCGRG 1 cut(s) 708
EcoICRI GAGCTC 1 cut(s) 364
EcoRI GAATTC 2 cut(s) 91, 553
EcoRII CCWGG 1 cut(s) 1540
EcoT14I CCWWGG 3 cut(s) 673, 1182, 1618
EcoT22I ATGCAT 2 cut(s) 278, 1485
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 673, 1182, 1618
FaeI CATG 9 cut(s) 243, 280, 410, 677, 682, 1037, 1487, 1571, 1627
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FatI CATG 9 cut(s) 239, 276, 406, 673, 678, 1033, 1483, 1567, 1623
FauI CCCGC 2 cut(s) 74, 614
FblI GTMKAC 1 cut(s) 563
Fnu4HI GCNGC 5 cut(s) 180, 341, 456, 873, 1653
FokI GGATG 5 cut(s) 143, 263, 869, 1328, 1492
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 5 cut(s) 180, 341, 456, 873, 1653
FspBI CTAG 3 cut(s) 18, 195, 1394
GlaI GCGC 1 cut(s) 659
GluI GCNGC 5 cut(s) 180, 341, 456, 873, 1653
GsaI CCCAGC 2 cut(s) 490, 1510
HaeIII GGCC 5 cut(s) 166, 291, 473, 792, 1734
HapII CCGG 3 cut(s) 745, 983, 1667
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 660
Hin1II CATG 9 cut(s) 243, 280, 410, 677, 682, 1037, 1487, 1571, 1627
Hin6I GCGC 1 cut(s) 658
HinP1I GCGC 1 cut(s) 658
HincII GTYRAC 2 cut(s) 939, 1638
HindII GTYRAC 2 cut(s) 939, 1638
HinfI GANTC 6 cut(s) 115, 191, 825, 940, 1610, 1761
HpaII CCGG 3 cut(s) 745, 983, 1667
Hpy166II GTNNAC 5 cut(s) 564, 580, 939, 1523, 1638
Hpy188I TCNGA 4 cut(s) 90, 120, 334, 1296
Hpy188III TCNNGA 8 cut(s) 195, 262, 734, 822, 829, 1520, 1568, 1727
Hpy8I GTNNAC 5 cut(s) 564, 580, 939, 1523, 1638
Hpy99I CGWCG 1 cut(s) 1769
HpyAV CCTTC 4 cut(s) 339, 1096, 1360, 1861
HpyCH4III ACNGT 3 cut(s) 1476, 1527, 1952
HpyCH4IV ACGT 1 cut(s) 1549
HpyCH4V TGCA 6 cut(s) 98, 182, 276, 1141, 1244, 1483
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 684, 1489
HpyF3I CTNAG 5 cut(s) 44, 1170, 1198, 1694, 1757
HpySE526I ACGT 1 cut(s) 1549
Hsp92II CATG 9 cut(s) 243, 280, 410, 677, 682, 1037, 1487, 1571, 1627
HspAI GCGC 1 cut(s) 658
Kzo9I GATC 7 cut(s) 197, 325, 669, 931, 1375, 1445, 1790
LmnI GCTCC 1 cut(s) 869
Lsp1109I GCAGC 3 cut(s) 166, 442, 859
LweI GCATC 4 cut(s) 652, 1033, 1150, 1470
MaeI CTAG 3 cut(s) 18, 195, 1394
MaeII ACGT 1 cut(s) 1549
MaeIII GTNAC 2 cut(s) 965, 1381
MalI GATC 7 cut(s) 199, 327, 671, 933, 1377, 1447, 1792
MboI GATC 7 cut(s) 197, 325, 669, 931, 1375, 1445, 1790
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 5 cut(s) 197, 325, 669, 1445, 1790
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 1 cut(s) 473
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 2 cut(s) 185, 934
MmeI TCCRAC 1 cut(s) 1488
MnlI CCTC 6 cut(s) 181, 406, 520, 677, 847, 1745
Mox20I TGGCCA 1 cut(s) 473
Mph1103I ATGCAT 2 cut(s) 278, 1485
MroXI GAANNNNTTC 5 cut(s) 601, 813, 1083, 1515, 1609
MscI TGGCCA 1 cut(s) 473
MseI TTAA 8 cut(s) 125, 537, 623, 1025, 1067, 1110, 1833, 1893
MslI CAYNNNNRTG 3 cut(s) 596, 648, 1628
Msp20I TGGCCA 1 cut(s) 473
MspI CCGG 3 cut(s) 745, 983, 1667
MspR9I CCNGG 2 cut(s) 984, 1542
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 1542
MwoI GCNNNNNNNGC 3 cut(s) 452, 684, 1489
NciI CCSGG 1 cut(s) 984
NcoI CCATGG 1 cut(s) 673
NdeII GATC 7 cut(s) 197, 325, 669, 931, 1375, 1445, 1790
NlaIII CATG 9 cut(s) 243, 280, 410, 677, 682, 1037, 1487, 1571, 1627
NlaIV GGNNCC 4 cut(s) 327, 344, 604, 671
NmuCI GTSAC 2 cut(s) 965, 1381
NsiI ATGCAT 2 cut(s) 278, 1485
NspI RCATGY 1 cut(s) 682
PaeI GCATGC 1 cut(s) 682
PaeR7I CTCGAG 1 cut(s) 708
PagI TCATGA 1 cut(s) 1567
PceI AGGCCT 1 cut(s) 166
PdmI GAANNNNTTC 5 cut(s) 601, 813, 1083, 1515, 1609
PfeI GAWTC 4 cut(s) 115, 825, 1610, 1761
PflMI CCANNNNNTGG 1 cut(s) 1624
PkrI GCNGC 5 cut(s) 181, 342, 457, 874, 1654
PleI GAGTC 2 cut(s) 185, 934
PpsI GAGTC 2 cut(s) 185, 934
Psp124BI GAGCTC 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 1540
PspFI CCCAGC 2 cut(s) 486, 1506
PspGI CCWGG 1 cut(s) 1540
PspN4I GGNNCC 4 cut(s) 327, 344, 604, 671
PspPI GGNCC 3 cut(s) 289, 747, 980
PspXI VCTCGAGB 1 cut(s) 708
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 5 cut(s) 197, 325, 669, 1445, 1790
RseI CAYNNNNRTG 3 cut(s) 596, 648, 1628
SacI GAGCTC 1 cut(s) 366
SaqAI TTAA 8 cut(s) 125, 537, 623, 1025, 1067, 1110, 1833, 1893
SatI GCNGC 5 cut(s) 180, 341, 456, 873, 1653
Sau3AI GATC 7 cut(s) 197, 325, 669, 931, 1375, 1445, 1790
Sau96I GGNCC 3 cut(s) 289, 747, 980
SchI GAGTC 2 cut(s) 185, 934
ScrFI CCNGG 2 cut(s) 984, 1542
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 4 cut(s) 652, 1033, 1150, 1470
Sfr274I CTCGAG 1 cut(s) 708
SinI GGWCC 2 cut(s) 747, 980
SlaI CTCGAG 1 cut(s) 708
SmiMI CAYNNNNRTG 3 cut(s) 596, 648, 1628
SmlI CTYRAG 3 cut(s) 143, 708, 1786
SmoI CTYRAG 3 cut(s) 143, 708, 1786
SphI GCATGC 1 cut(s) 682
SseBI AGGCCT 1 cut(s) 166
SsiI CCGC 6 cut(s) 67, 340, 607, 641, 889, 1652
SspI AATATT 2 cut(s) 1335, 1960
SspMI CTAG 3 cut(s) 18, 195, 1394
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 166
StyD4I CCNGG 2 cut(s) 982, 1540
StyI CCWWGG 3 cut(s) 673, 1182, 1618
TaaI ACNGT 3 cut(s) 1476, 1527, 1952
TaiI ACGT 1 cut(s) 1552
TaqI TCGA 2 cut(s) 709, 1767
TauI GCSGC 2 cut(s) 343, 1655
TfiI GAWTC 4 cut(s) 115, 825, 1610, 1761
Tru1I TTAA 8 cut(s) 125, 537, 623, 1025, 1067, 1110, 1833, 1893
Tru9I TTAA 8 cut(s) 125, 537, 623, 1025, 1067, 1110, 1833, 1893
TscAI CASTG 1 cut(s) 1955
TseFI GTSAC 2 cut(s) 965, 1381
TseI GCWGC 3 cut(s) 179, 455, 872
Tsp45I GTSAC 2 cut(s) 965, 1381
TspGWI ACGGA 2 cut(s) 547, 719
TspRI CASTG 1 cut(s) 1955
Van91I CCANNNNNTGG 1 cut(s) 1624
VpaK11BI GGWCC 2 cut(s) 747, 980
XapI RAATTY 6 cut(s) 91, 553, 1115, 1681, 1804, 1971
XbaI TCTAGA 1 cut(s) 194
XceI RCATGY 1 cut(s) 682
XcmI CCANNNNNNNNNTGG 1 cut(s) 829
XhoI CTCGAG 1 cut(s) 708
XmiI GTMKAC 1 cut(s) 563
XmnI GAANNNNTTC 5 cut(s) 601, 813, 1083, 1515, 1609
XspI CTAG 3 cut(s) 18, 195, 1394
Zsp2I ATGCAT 2 cut(s) 278, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.