RLG00000030367

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000105
Physical Location & Seq
Forward (+)
60162019 .. 60174318
12300 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030367

Sequence Viewer

Length: 3420 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGGGGGTTAGATAGCCGTAGGAGTTTTACCGGTTATCTGTATTCTGCACTAAGACAGAAAGGAATTGTCACCTTTATGGATGAGCCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGGGGCAATTGAGGACTCCAGATCTGCGATTGTCATTCTCTCAAAGAGATATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATGGGCCAACAAGTCCTCCCCGTCTTCTACAAGGTGGATCCTTCTGATGTGAGGCACCAAAGGGGAGATTTTGAGCTCAAATGGGAAACACAAGTAGAAGTACAAGTAAGGGACCATGAAGAAGTTTATGGGATTGCTGAGGACAGACTAAATGCCTGGAGAGCTGCTTTGACACAGGTCGCCAATCTTTCTGGCTGGGATTTCAACAATTACAACGGCTTCAAAGCAAATCTAGTTGACGAAATTGTTCATCAAATACTAAAGAAGTCCGTGCATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTCATGATTTATTAAGCAATTACATATATCCGCAGCTTGGTGGGGTGGTGCGTTTTACAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTGTCCATGATGAAATTTGTCAGGATTTTGACCGGAGCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAAGAACGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAAGTTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATCGAAAGGCGGTCGTGTAAAATGAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGATGTCGCAACTGGTTTGGTCCAGGGAGTAGAATCATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGTTAAAGGGTTAAACCATGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAGGAATTGTCCCCCACCAGAAGATTATTTGCATCTGTGCAACCTTATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTCTTTGGTAGAAGCACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGAGGTGCTTCGAATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACGCAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTCTTCTTTCTGATAGATCTCTCATAACTATCTCCAACAATGAAGTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCTACAGAACCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAAAAATAAGAATTTTTACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTGCGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCAAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAGCATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGACTTTGTTGGAAATATGGAACGTTTGTTAGTGCTTTGTCTTGATGAGACTGCTATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTTTGTCATTGAATTTAAGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAATTGAAGTCTCTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTAGGGGAGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCGATGATCGCAGAAAATTTGTGAGAGGTTCAATCTTTCATGGATGGAAAGTAGTTTGGGGATCTTTAAAAAAGTTCTTGCCTTCTCGAGTAGTGCAAAAAGTGAATACAGAGCCAATGAGTTTCCGCTTGCCTATATCTGGTCTATGTAATTTAACATATCTGAACCTGAGTAATTCCAATCTTAGTGAAGGAGCATTTGCCAATGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTTGCCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTTGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGACTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGCTCTTGAAACTAGTCCTCCGGAGCAACATTCTCTTCCAGAGCTGCATTCTCCTCATGAACAGCAACAAATTGACTCAGATCTCTTTTCTTCAGATTATGATGGGAGCTTTCTGGATGGTTTTTTAGATCTCAATAGCTCTGATGATGAGGTAACCATCGATCCTACATCTGAGGCGGAGACGAAACGTATGTCCTTGGCTAAAATGGGGTACAATGAATTGGAGGCTTCAACGGCAATAGAAAGATGCGTTTATGTACACATTCCATGTCTGGTAGATTATGACTGCTGGTTCAACTTTTACAGCGTACACATCTGCGGAGAAAAGGATATGGGACATGGAAACCAGATCATAAGGGAAGATGCTGGTACAATTCATCTCCCGAATCCAATGAATGGGTTTGGGACTCCAGCTGATCTCTGTCAAATCCACAGAAGTCTTCCAGAGTCGGCCACAGGACCTCCTTATTTTTACTATGAGAATGTGGCATTGACTCCTAAAGGAGTTTGGAGCACAATACCCCACTTTTTGTATGATGTGAAGCCTGAGTTTGTTGATCCGAAGTATTTTTGTGCTGCTGCAAGAAAAAGGGGCTATGTTCACAATCTTCCAATCCAGACTTCCAATCAAACTATATTTGATGCATTTCCCATGACAAGGAGGCGGTGGCCTACTTGGGATGATTGGACAAAGCTAAATTACTTACAGACTTGCATTAATTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1140

Amino Acids

129.23

Weight (kDa)

5.95

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 192 4.9e-48 TIR domain
TIR_2 PF13676 14 - 116 3.3e-14 TIR domain
NB-ARC PF00931 222 - 373 7.4e-18 NB-ARC domain
NACHT PF05729 225 - 366 6.3e-08 NACHT domain
WHD_ROQ1 PF23282 440 - 511 6e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 608 - 692 4.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 615 - 688 1e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1664
Acc36I ACCTGC 1 cut(s) 1664
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 1 cut(s) 3089
AccI GTMKAC 1 cut(s) 2524
AccIII TCCGGA 1 cut(s) 2713
AciI CCGC 8 cut(s) 607, 641, 685, 892, 2287, 2870, 3012, 3358
AclI AACGTT 1 cut(s) 1897
AclWI GGATC 9 cut(s) 320, 333, 667, 680, 942, 1386, 2230, 2849, 3245
AcoI YGGCCR 1 cut(s) 3144
AcsI RAATTY 7 cut(s) 729, 1606, 1717, 1981, 2177, 2369, 2581
AcuI CTGAAG 4 cut(s) 1613, 2418, 2696, 2769
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 390, 2551, 2906, 2952, 3003, 3064
AfiI CCNNNNNNNGG 5 cut(s) 647, 1424, 2300, 3089, 3351
AgeI ACCGGT 1 cut(s) 83
AhlI ACTAGT 1 cut(s) 2705
AjnI CCWGG 3 cut(s) 443, 985, 1543
AjuI GAANNNNNNNTTGG 4 cut(s) 2456, 2488, 3298, 3330
Alw21I GWGCWC 2 cut(s) 366, 3209
Alw26I GTCTC 9 cut(s) 1536, 1572, 1652, 1685, 1796, 1916, 2043, 2482, 2867
AlwI GGATC 9 cut(s) 320, 333, 667, 680, 942, 1386, 2230, 2849, 3245
AlwNI CAGNNNCTG 1 cut(s) 875
Ama87I CYCGRG 2 cut(s) 711, 2247
Aor13HI TCCGGA 1 cut(s) 2713
AoxI GGCC 6 cut(s) 167, 292, 793, 1966, 3144, 3362
ApeKI GCWGC 7 cut(s) 452, 643, 753, 875, 2737, 3269, 3272
ApoI RAATTY 7 cut(s) 729, 1606, 1717, 1981, 2177, 2369, 2581
ArsI GACNNNNNNTTYG 4 cut(s) 1954, 1986, 2870, 2902
AseI ATTAAT 2 cut(s) 1838, 3411
AsiGI ACCGGT 1 cut(s) 83
Asp700I GAANNNNTTC 6 cut(s) 534, 601, 816, 1086, 1518, 2681
AspS9I GGNCC 4 cut(s) 292, 400, 983, 3152
AsuHPI GGTGA 2 cut(s) 115, 2404
AsuII TTCGAA 1 cut(s) 1297
AvaI CYCGRG 2 cut(s) 711, 2247
AvaII GGWCC 3 cut(s) 400, 983, 3152
BamHI GGATCC 2 cut(s) 325, 672
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1193
BbsI GAAGAC 6 cut(s) 304, 1427, 1581, 2003, 2684, 3125
Bbv12I GWGCWC 2 cut(s) 366, 3209
BbvCI CCTCAGC 1 cut(s) 426
BbvI GCAGC 7 cut(s) 439, 655, 740, 862, 2724, 3256, 3259
BceAI ACGGC 5 cut(s) 54, 520, 808, 1973, 2943
BciT130I CCWGG 3 cut(s) 445, 987, 1545
BcoDI GTCTC 9 cut(s) 1536, 1572, 1652, 1685, 1796, 1916, 2043, 2482, 2867
BcuI ACTAGT 1 cut(s) 2705
BfaI CTAG 5 cut(s) 521, 797, 1397, 2706, 3418
BfmI CTRYAG 2 cut(s) 1536, 1993
BfuAI ACCTGC 1 cut(s) 1664
BglII AGATCT 5 cut(s) 200, 1448, 1699, 2773, 2821
BisI GCNGC 7 cut(s) 453, 644, 754, 876, 2738, 3270, 3273
BlsI GCNGC 7 cut(s) 454, 645, 755, 877, 2739, 3271, 3274
Bme1390I CCNGG 3 cut(s) 445, 987, 1545
Bme18I GGWCC 3 cut(s) 400, 983, 3152
BmeT110I CYCGRG 2 cut(s) 711, 2247
BmgT120I GGNCC 4 cut(s) 292, 400, 983, 3152
BmiI GGNNCC 4 cut(s) 327, 344, 401, 674
BmrFI CCNGG 3 cut(s) 445, 987, 1545
BoxI GACNNNNGTC 1 cut(s) 464
BpiI GAAGAC 6 cut(s) 304, 1427, 1581, 2003, 2684, 3125
BpmI CTGGAG 3 cut(s) 181, 466, 3087
Bpu10I CCTNAGC 2 cut(s) 426, 1173
Bpu14I TTCGAA 1 cut(s) 1297
BpuEI CTTGAG 4 cut(s) 167, 1709, 1778, 2480
Bsa29I ATCGAT 1 cut(s) 2853
BsaBI GATNNNNATC 2 cut(s) 933, 1377
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1572, 1652
BsaJI CCNNGG 4 cut(s) 676, 986, 1741, 2889
BsaWI WCCGGW 3 cut(s) 83, 747, 2713
BsaXI ACNNNNNCTCC 6 cut(s) 288, 318, 2695, 2725, 3139, 3169
Bsc4I CCNNNNNNNGG 5 cut(s) 647, 1424, 2300, 3089, 3351
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 3 cut(s) 980, 1320, 1969
Bse8I GATNNNNATC 2 cut(s) 933, 1377
BseAI TCCGGA 1 cut(s) 2713
BseBI CCWGG 3 cut(s) 445, 987, 1545
BseCI ATCGAT 1 cut(s) 2853
BseDI CCNNGG 4 cut(s) 676, 986, 1741, 2889
BseJI GATNNNNATC 2 cut(s) 933, 1377
BseLI CCNNNNNNNGG 5 cut(s) 647, 1424, 2300, 3089, 3351
BseMII CTCAG 6 cut(s) 417, 1187, 2321, 2784, 2856, 3231
BseNI ACTGG 3 cut(s) 980, 1320, 1969
BseRI GAGGAG 1 cut(s) 2736
BseXI GCAGC 7 cut(s) 439, 655, 740, 862, 2724, 3256, 3259
BseYI CCCAGC 2 cut(s) 483, 1509
BsgI GTGCAG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 896
BshFI GGCC 6 cut(s) 169, 294, 795, 1968, 3146, 3364
BshNI GGYRCC 1 cut(s) 342
BshTI ACCGGT 1 cut(s) 83
BshVI ATCGAT 1 cut(s) 2853
BsiEI CGRYCG 1 cut(s) 896
BsiHKAI GWGCWC 2 cut(s) 366, 3209
BsiHKCI CYCGRG 2 cut(s) 711, 2247
BsiSI CCGG 3 cut(s) 84, 748, 2714
BslFI GGGAC 4 cut(s) 413, 1107, 3042, 3112
BslI CCNNNNNNNGG 5 cut(s) 647, 1424, 2300, 3089, 3351
BsmAI GTCTC 9 cut(s) 1536, 1572, 1652, 1685, 1796, 1916, 2043, 2482, 2867
BsmBI CGTCTC 1 cut(s) 2867
BsmFI GGGAC 4 cut(s) 413, 1107, 3042, 3112
BsmI GAATGC 2 cut(s) 609, 2740
BsnI GGCC 6 cut(s) 169, 294, 795, 1968, 3146, 3364
Bso31I GGTCTC 2 cut(s) 1572, 1652
BsoBI CYCGRG 2 cut(s) 711, 2247
Bsp119I TTCGAA 1 cut(s) 1297
Bsp1286I GDGCHC 2 cut(s) 366, 3209
Bsp13I TCCGGA 1 cut(s) 2713
Bsp1407I TGTACA 1 cut(s) 2950
Bsp19I CCATGG 1 cut(s) 676
BspACI CCGC 8 cut(s) 607, 641, 685, 892, 2287, 2870, 3012, 3358
BspANI GGCC 6 cut(s) 169, 294, 795, 1968, 3146, 3364
BspCNI CTCAG 6 cut(s) 418, 1186, 2322, 2783, 2857, 3232
BspDI ATCGAT 1 cut(s) 2853
BspEI TCCGGA 1 cut(s) 2713
BspHI TCATGA 3 cut(s) 613, 1570, 2749
BspLI GGNNCC 4 cut(s) 327, 344, 401, 674
BspMAI CTGCAG 1 cut(s) 1997
BspMI ACCTGC 1 cut(s) 1664
BspPI GGATC 9 cut(s) 320, 333, 667, 680, 942, 1386, 2230, 2849, 3245
BspT104I TTCGAA 1 cut(s) 1297
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 2 cut(s) 1572, 1652
BsrFI RCCGGY 1 cut(s) 83
BsrGI TGTACA 1 cut(s) 2950
BsrI ACTGG 3 cut(s) 980, 1320, 1969
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 4 cut(s) 676, 986, 1741, 2889
BssNAI GTATAC 1 cut(s) 2525
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1193
BssT1I CCWWGG 3 cut(s) 676, 1741, 2889
Bst1107I GTATAC 1 cut(s) 2525
Bst2BI CACGAG 1 cut(s) 1193
Bst2UI CCWGG 3 cut(s) 445, 987, 1545
Bst4CI ACNGT 2 cut(s) 1530, 2111
Bst6I CTCTTC 2 cut(s) 19, 2733
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 1492, 3414
BstAUI TGTACA 1 cut(s) 2950
BstBI TTCGAA 1 cut(s) 1297
BstC8I GCNNGC 4 cut(s) 683, 1354, 1409, 2291
BstDSI CCRYGG 1 cut(s) 676
BstEII GGTNACC 2 cut(s) 2392, 2845
BstMAI GTCTC 9 cut(s) 1536, 1572, 1652, 1685, 1796, 1916, 2043, 2482, 2867
BstMCI CGRYCG 1 cut(s) 896
BstMWI GCNNNNNNNGC 6 cut(s) 449, 687, 1492, 2455, 2927, 3414
BstNI CCWGG 3 cut(s) 445, 987, 1545
BstNSI RCATGY 1 cut(s) 685
BstPAI GACNNNNGTC 1 cut(s) 464
BstPI GGTNACC 2 cut(s) 2392, 2845
BstSCI CCNGG 3 cut(s) 443, 985, 1543
BstSFI CTRYAG 2 cut(s) 1536, 1993
BstV1I GCAGC 7 cut(s) 439, 655, 740, 862, 2724, 3256, 3259
BstV2I GAAGAC 6 cut(s) 304, 1427, 1581, 2003, 2684, 3125
BstX2I RGATCY 8 cut(s) 200, 325, 672, 1448, 1699, 2222, 2773, 2821
BstYI RGATCY 8 cut(s) 200, 325, 672, 1448, 1699, 2222, 2773, 2821
BstZ17I GTATAC 1 cut(s) 2525
Bsu15I ATCGAT 1 cut(s) 2853
BsuRI GGCC 6 cut(s) 169, 294, 795, 1968, 3146, 3364
BsuTUI ATCGAT 1 cut(s) 2853
BtgI CCRYGG 1 cut(s) 676
BtsIMutI CAGTG 2 cut(s) 1227, 2555
BveI ACCTGC 1 cut(s) 1664
Cac8I GCNNGC 4 cut(s) 683, 1354, 1409, 2291
CaiI CAGNNNCTG 1 cut(s) 875
CciI TCATGA 3 cut(s) 613, 1570, 2749
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 4 cut(s) 292, 400, 983, 3152
ClaI ATCGAT 1 cut(s) 2853
CseI GACGC 1 cut(s) 1396
Csp6I GTAC 6 cut(s) 389, 2550, 2905, 2951, 3002, 3063
CspAI ACCGGT 1 cut(s) 83
CviQI GTAC 6 cut(s) 389, 2550, 2905, 2951, 3002, 3063
DraI TTTAAA 1 cut(s) 2229
EaeI YGGCCR 1 cut(s) 3144
Eam1104I CTCTTC 2 cut(s) 19, 2733
EarI CTCTTC 2 cut(s) 19, 2733
EciI GGCGGA 1 cut(s) 2885
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 676, 1741, 2889
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 2 cut(s) 1572, 1652
Eco47I GGWCC 3 cut(s) 400, 983, 3152
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 4 cut(s) 1613, 2418, 2696, 2769
Eco88I CYCGRG 2 cut(s) 711, 2247
Eco91I GGTNACC 2 cut(s) 2392, 2845
EcoICRI GAGCTC 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 3152
EcoO65I GGTNACC 2 cut(s) 2392, 2845
EcoRII CCWGG 3 cut(s) 443, 985, 1543
EcoT14I CCWWGG 3 cut(s) 676, 1741, 2889
EcoT22I ATGCAT 4 cut(s) 281, 1488, 2578, 3340
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 676, 1741, 2889
Esp3I CGTCTC 1 cut(s) 2867
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 4 cut(s) 413, 1107, 3042, 3112
FauI CCCGC 2 cut(s) 614, 678
FblI GTMKAC 1 cut(s) 2524
Fnu4HI GCNGC 7 cut(s) 453, 644, 754, 876, 2738, 3270, 3273
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 7 cut(s) 453, 644, 754, 876, 2738, 3270, 3273
FspBI CTAG 5 cut(s) 521, 797, 1397, 2706, 3418
GluI GCNGC 7 cut(s) 453, 644, 754, 876, 2738, 3270, 3273
GsaI CCCAGC 2 cut(s) 487, 1513
GsuI CTGGAG 3 cut(s) 181, 466, 3087
HaeIII GGCC 6 cut(s) 169, 294, 795, 1968, 3146, 3364
HapII CCGG 3 cut(s) 84, 748, 2714
HgaI GACGC 1 cut(s) 1396
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 4 cut(s) 9, 526, 580, 942
HindII GTYRAC 4 cut(s) 9, 526, 580, 942
HindIII AAGCTT 2 cut(s) 1757, 2456
HpaII CCGG 3 cut(s) 84, 748, 2714
HphI GGTGA 2 cut(s) 115, 2404
Hpy166II GTNNAC 9 cut(s) 9, 526, 580, 942, 1526, 2525, 2953, 3004, 3295
Hpy8I GTNNAC 9 cut(s) 9, 526, 580, 942, 1526, 2525, 2953, 3004, 3295
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 2 cut(s) 1530, 2111
HpyCH4IV ACGT 3 cut(s) 1552, 1897, 2881
HpyF10VI GCNNNNNNNGC 6 cut(s) 449, 687, 1492, 2455, 2927, 3414
HpySE526I ACGT 3 cut(s) 1552, 1897, 2881
Hsp92I GRCGYC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 2713
LmnI GCTCC 6 cut(s) 750, 872, 2354, 2716, 2799, 3204
Lsp1109I GCAGC 7 cut(s) 439, 655, 740, 862, 2724, 3256, 3259
MaeI CTAG 5 cut(s) 521, 797, 1397, 2706, 3418
MaeII ACGT 3 cut(s) 1552, 1897, 2881
MaeIII GTNAC 5 cut(s) 121, 1384, 2375, 2392, 2845
MfeI CAATTG 3 cut(s) 186, 1950, 2620
MflI RGATCY 8 cut(s) 200, 325, 672, 1448, 1699, 2222, 2773, 2821
MhlI GDGCHC 2 cut(s) 366, 3209
MlyI GAGTC 6 cut(s) 188, 1807, 2762, 3094, 3149, 3181
MmeI TCCRAC 2 cut(s) 1491, 1864
Mph1103I ATGCAT 4 cut(s) 281, 1488, 2578, 3340
MroI TCCGGA 1 cut(s) 2713
MroXI GAANNNNTTC 6 cut(s) 534, 601, 816, 1086, 1518, 2681
MslI CAYNNNNRTG 2 cut(s) 128, 596
MspA1I CMGCKG 1 cut(s) 3107
MspI CCGG 3 cut(s) 84, 748, 2714
MspR9I CCNGG 3 cut(s) 445, 987, 1545
MunI CAATTG 3 cut(s) 186, 1950, 2620
Mva1269I GAATGC 2 cut(s) 609, 2740
MvaI CCWGG 3 cut(s) 445, 987, 1545
MwoI GCNNNNNNNGC 6 cut(s) 449, 687, 1492, 2455, 2927, 3414
NcoI CCATGG 1 cut(s) 676
NlaIV GGNNCC 4 cut(s) 327, 344, 401, 674
NmeAIII GCCGAG 1 cut(s) 2055
NmuCI GTSAC 3 cut(s) 121, 1384, 2392
NsiI ATGCAT 4 cut(s) 281, 1488, 2578, 3340
NspI RCATGY 1 cut(s) 685
NspV TTCGAA 1 cut(s) 1297
PaeI GCATGC 1 cut(s) 685
PaeR7I CTCGAG 2 cut(s) 711, 2247
PagI TCATGA 3 cut(s) 613, 1570, 2749
PaqCI CACCTGC 1 cut(s) 1664
PceI AGGCCT 1 cut(s) 169
PctI GAATGC 2 cut(s) 609, 2740
PdmI GAANNNNTTC 6 cut(s) 534, 601, 816, 1086, 1518, 2681
PflMI CCANNNNNTGG 1 cut(s) 3089
PinAI ACCGGT 1 cut(s) 83
PkrI GCNGC 7 cut(s) 454, 645, 755, 877, 2739, 3271, 3274
PleI GAGTC 6 cut(s) 188, 1806, 2762, 3094, 3148, 3181
PpsI GAGTC 6 cut(s) 188, 1806, 2762, 3094, 3148, 3181
PpuMI RGGWCCY 1 cut(s) 3152
PshAI GACNNNNGTC 1 cut(s) 464
PshBI ATTAAT 2 cut(s) 1838, 3411
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 1897
Psp5II RGGWCCY 1 cut(s) 3152
Psp6I CCWGG 3 cut(s) 443, 985, 1543
PspEI GGTNACC 2 cut(s) 2392, 2845
PspFI CCCAGC 2 cut(s) 483, 1509
PspGI CCWGG 3 cut(s) 443, 985, 1543
PspN4I GGNNCC 4 cut(s) 327, 344, 401, 674
PspPI GGNCC 4 cut(s) 292, 400, 983, 3152
PspPPI RGGWCCY 1 cut(s) 3152
PspXI VCTCGAGB 1 cut(s) 711
PstI CTGCAG 1 cut(s) 1997
PstNI CAGNNNCTG 1 cut(s) 875
PsuI RGATCY 8 cut(s) 200, 325, 672, 1448, 1699, 2222, 2773, 2821
PvuII CAGCTG 1 cut(s) 3107
RsaI GTAC 6 cut(s) 390, 2551, 2906, 2952, 3003, 3064
RsaNI GTAC 6 cut(s) 389, 2550, 2905, 2951, 3002, 3063
RseI CAYNNNNRTG 2 cut(s) 128, 596
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 7 cut(s) 453, 644, 754, 876, 2738, 3270, 3273
Sau96I GGNCC 4 cut(s) 292, 400, 983, 3152
SchI GAGTC 6 cut(s) 188, 1807, 2762, 3094, 3149, 3181
ScrFI CCNGG 3 cut(s) 445, 987, 1545
SduI GDGCHC 2 cut(s) 366, 3209
SfcI CTRYAG 2 cut(s) 1536, 1993
Sfr274I CTCGAG 2 cut(s) 711, 2247
SfuI TTCGAA 1 cut(s) 1297
SinI GGWCC 3 cut(s) 400, 983, 3152
SlaI CTCGAG 2 cut(s) 711, 2247
SmiMI CAYNNNNRTG 2 cut(s) 128, 596
SmlI CTYRAG 6 cut(s) 146, 711, 1688, 1793, 2247, 2459
SmoI CTYRAG 6 cut(s) 146, 711, 1688, 1793, 2247, 2459
SpeI ACTAGT 1 cut(s) 2705
SphI GCATGC 1 cut(s) 685
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 8 cut(s) 607, 641, 685, 892, 2287, 2870, 3012, 3358
SspI AATATT 1 cut(s) 1338
SspMI CTAG 5 cut(s) 521, 797, 1397, 2706, 3418
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 3 cut(s) 443, 985, 1543
StyI CCWWGG 3 cut(s) 676, 1741, 2889
TaaI ACNGT 2 cut(s) 1530, 2111
TaiI ACGT 3 cut(s) 1555, 1900, 2884
TaqI TCGA 5 cut(s) 712, 885, 1297, 2248, 2853
TatI WGTACW 3 cut(s) 388, 2549, 2950
TscAI CASTG 2 cut(s) 1234, 2562
TseFI GTSAC 3 cut(s) 121, 1384, 2392
TseI GCWGC 7 cut(s) 452, 643, 753, 875, 2737, 3269, 3272
Tsp45I GTSAC 3 cut(s) 121, 1384, 2392
TspGWI ACGGA 2 cut(s) 547, 1173
TspRI CASTG 2 cut(s) 1234, 2562
Van91I CCANNNNNTGG 1 cut(s) 3089
VpaK11BI GGWCC 3 cut(s) 400, 983, 3152
VspI ATTAAT 2 cut(s) 1838, 3411
XapI RAATTY 7 cut(s) 729, 1606, 1717, 1981, 2177, 2369, 2581
XceI RCATGY 1 cut(s) 685
XcmI CCANNNNNNNNNTGG 2 cut(s) 832, 1739
XhoI CTCGAG 2 cut(s) 711, 2247
XmiI GTMKAC 1 cut(s) 2524
XmnI GAANNNNTTC 6 cut(s) 534, 601, 816, 1086, 1518, 2681
XspI CTAG 5 cut(s) 521, 797, 1397, 2706, 3418
Zsp2I ATGCAT 4 cut(s) 281, 1488, 2578, 3340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.