Rroxscaffold_4G00327260

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
59793853 .. 59798796
4944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327260.1

Sequence Viewer

Length: 1812 bp
ATGGATTTTTTCCACCATTCAATTAAAGCTACTATTCAATACAACAAGGCCGGCCCCGAGGGCTGCTACTTGAGGTGGCCGCCTCGTGCCACGGCCATCGGCGGCCTCTGTCGCCCATCGCCGGAGCCCTCGCATACTCGCCACCGGCGCTGGGTCCTCCTCACCATCCCCGTCGCCGACGACTCTCCTCCTCCGCCGCAGTCCACGGCTCGCCCGGATCTCAACCTCGACCACCTCCTCTCTCCTAGCGCTCCGACCTCGACAAGCACCTCTCCAATCTCTAAAAATCCTCCCGGTGTCCTCGACATCGTCAAGTCCGACGCCGACCACGTCCTCTCCAACGTCTCCTCCACCTGCGACCTCGCCTACCACGTCGCACCAAGACATCAAACAGAACTAATTGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTGATAGGAATGGAGTCCCGCATTGATGATTTATTAAGCAATTACATATTTGCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACAGTTGCTCGAGCTATCTATGATGAAATTTGTCAGGGTTTTTACCGAAGCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTTGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAAGTTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGTCACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATTATCATAACCACCACAGATATTCAGTTGTTAAAGGCACATGATGTTGATGCTACGTACAAGGCTTATGGGTTAAACTGTGATGAAACACTGCAACTTTTGAGTTTGAAGGCTTTTAAGAAGTGTTCCCCACCAGAAGATTATTTGCAACTGTGCTACCATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGAGGTGCTTCGGATTAGTTTTGATGGCCTGGATGAAAAAGACAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGAGTGACACAAATACTAGACTATTGCAAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATGTCTCATCACTATCTCCAACAACGAACTATCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTGAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACACTGTGCTGAAGAGAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTGAATTACAAGTGGCTCATTGGATGCCAAAAGCCTTTTCAAATTTGTCTCAACTTATTCTACTCCATATTCATAATGTGGACCTTCCTGAAGGCCTCACTTGTCTCTCTAATTCCTTGAGACTCCTCGAATGGACGGGGTATCCCTTAAGATCTCTCCCAAAAAAATTTGAAGCAGATGAACTCATTGAACTTAACTTGTGCCGAAGCAACATTAAACAGCTTTGGAAGGGAACAAAGCAAATTGTCTGTTCTGTGTGGTGGCTCACTGTTTCGATTTTATGGGCAGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

603

Amino Acids

68.01

Weight (kDa)

6.54

Isoelectric Point (pI)

56.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 176 - 331 1.3e-16 NB-ARC domain
NACHT PF05729 181 - 277 2.2e-06 NACHT domain
WHD_ROQ1 PF23282 398 - 469 3.6e-22 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 362
Acc16I TGCGCA 1 cut(s) 519
Acc36I ACCTGC 1 cut(s) 362
AccB7I CCANNNNNTGG 1 cut(s) 1501
AccI GTMKAC 1 cut(s) 440
AciI CCGC 7 cut(s) 80, 102, 194, 197, 484, 559, 766
AclWI GGATC 5 cut(s) 225, 541, 554, 816, 1260
AcoI YGGCCR 2 cut(s) 77, 93
AcsI RAATTY 4 cut(s) 603, 1558, 1682, 1805
AcuI CTGAAG 3 cut(s) 1431, 1487, 1626
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 929
AfeI AGCGCT 1 cut(s) 250
AfiI CCNNNNNNNGG 4 cut(s) 121, 151, 1298, 1501
AflII CTTAAG 1 cut(s) 1663
AgsI TTSAA 9 cut(s) 21, 38, 450, 722, 979, 1491, 1557, 1688, 1706
AjiI CACGTC 2 cut(s) 331, 373
AjnI CCWGG 3 cut(s) 859, 1188, 1417
AjuI GAANNNNNNNTTGG 2 cut(s) 1537, 1569
AluBI AGCT 7 cut(s) 29, 523, 590, 627, 749, 1285, 1738
AluI AGCT 7 cut(s) 29, 523, 590, 627, 749, 1285, 1738
Alw26I GTCTC 7 cut(s) 349, 1331, 1410, 1446, 1569, 1625, 1630
AlwI GGATC 5 cut(s) 225, 541, 554, 816, 1260
AlwNI CAGNNNCTG 1 cut(s) 749
Ama87I CYCGRG 2 cut(s) 56, 585
Aor51HI AGCGCT 1 cut(s) 250
AoxI GGCC 8 cut(s) 48, 52, 77, 93, 103, 667, 1186, 1609
ApeKI GCWGC 4 cut(s) 63, 520, 627, 749
ApoI RAATTY 4 cut(s) 603, 1558, 1682, 1805
Asp700I GAANNNNTTC 3 cut(s) 690, 1392, 1486
AspLEI GCGC 3 cut(s) 150, 251, 520
AspS9I GGNCC 4 cut(s) 53, 154, 857, 1597
AsuC2I CCSGG 2 cut(s) 215, 294
AsuHPI GGTGA 1 cut(s) 154
AvaI CYCGRG 2 cut(s) 56, 585
AvaII GGWCC 3 cut(s) 154, 857, 1597
BamHI GGATCC 1 cut(s) 546
BanII GRGCYC 1 cut(s) 129
BauI CACGAG 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 1455
BbvI GCAGC 4 cut(s) 50, 532, 614, 736
BccI CCATC 5 cut(s) 104, 124, 173, 703, 1178
BceAI ACGGC 2 cut(s) 108, 222
BciT130I CCWGG 3 cut(s) 861, 1190, 1419
BciVI GTATCC 1 cut(s) 1668
BcnI CCSGG 2 cut(s) 215, 294
BcoDI GTCTC 7 cut(s) 349, 1331, 1410, 1446, 1569, 1625, 1630
BfaI CTAG 2 cut(s) 246, 1271
BfoI RGCGCY 2 cut(s) 151, 252
BfrI CTTAAG 1 cut(s) 1663
BfuAI ACCTGC 1 cut(s) 362
BfuI GTATCC 1 cut(s) 1668
BglI GCCNNNNNGGC 1 cut(s) 60
BglII AGATCT 1 cut(s) 1667
BisI GCNGC 7 cut(s) 64, 80, 103, 197, 521, 628, 750
BlsI GCNGC 7 cut(s) 65, 81, 104, 198, 522, 629, 751
Bme1390I CCNGG 5 cut(s) 215, 294, 861, 1190, 1419
Bme18I GGWCC 3 cut(s) 154, 857, 1597
BmeT110I CYCGRG 2 cut(s) 56, 585
BmgBI CACGTC 2 cut(s) 331, 373
BmgT120I GGNCC 4 cut(s) 53, 154, 857, 1597
BmiI GGNNCC 4 cut(s) 55, 126, 155, 548
BmrFI CCNGG 5 cut(s) 215, 294, 861, 1190, 1419
BmsI GCATC 3 cut(s) 910, 1347, 1530
BpiI GAAGAC 1 cut(s) 1455
Bpu10I CCTNAGC 1 cut(s) 1047
BpuEI CTTGAG 2 cut(s) 91, 1654
BpuMI CCSGG 2 cut(s) 215, 294
Bsa29I ATCGAT 1 cut(s) 1355
BsaAI YACGTR 1 cut(s) 927
BsaBI GATNNNNATC 2 cut(s) 807, 1251
BsaHI GRCGYC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 1446
BsaJI CCNNGG 6 cut(s) 57, 90, 204, 550, 860, 1495
BsaXI ACNNNNNCTCC 4 cut(s) 320, 332, 350, 362
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 151, 1298, 1501
Bse118I RCCGGY 2 cut(s) 50, 144
Bse1I ACTGG 1 cut(s) 854
Bse8I GATNNNNATC 2 cut(s) 807, 1251
BseBI CCWGG 3 cut(s) 861, 1190, 1419
BseCI ATCGAT 1 cut(s) 1355
BseDI CCNNGG 6 cut(s) 57, 90, 204, 550, 860, 1495
BseGI GGATG 4 cut(s) 165, 739, 1198, 1545
BseJI GATNNNNATC 2 cut(s) 807, 1251
BseLI CCNNNNNNNGG 4 cut(s) 121, 151, 1298, 1501
BseMII CTCAG 1 cut(s) 1061
BseNI ACTGG 1 cut(s) 854
BseRI GAGGAG 6 cut(s) 149, 177, 180, 227, 337, 1631
BseXI GCAGC 4 cut(s) 50, 532, 614, 736
BseYI CCCAGC 2 cut(s) 150, 1383
BshFI GGCC 8 cut(s) 50, 54, 79, 95, 105, 669, 1188, 1611
BshVI ATCGAT 1 cut(s) 1355
BsiHKCI CYCGRG 2 cut(s) 56, 585
BsiSI CCGG 5 cut(s) 51, 122, 145, 215, 294
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 4 cut(s) 121, 151, 1298, 1501
BsmAI GTCTC 7 cut(s) 349, 1331, 1410, 1446, 1569, 1625, 1630
BsmBI CGTCTC 1 cut(s) 349
BsmFI GGGAC 1 cut(s) 466
BsnI GGCC 8 cut(s) 50, 54, 79, 95, 105, 669, 1188, 1611
Bso31I GGTCTC 1 cut(s) 1446
BsoBI CYCGRG 2 cut(s) 56, 585
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 5 cut(s) 217, 546, 808, 1252, 1667
Bsp19I CCATGG 1 cut(s) 550
BspACI CCGC 7 cut(s) 80, 102, 194, 197, 484, 559, 766
BspANI GGCC 8 cut(s) 50, 54, 79, 95, 105, 669, 1188, 1611
BspCNI CTCAG 1 cut(s) 1060
BspDI ATCGAT 1 cut(s) 1355
BspHI TCATGA 1 cut(s) 1444
BspLI GGNNCC 4 cut(s) 55, 126, 155, 548
BspMI ACCTGC 1 cut(s) 362
BspPI GGATC 5 cut(s) 225, 541, 554, 816, 1260
BspQI GCTCTTC 1 cut(s) 1407
BspTI CTTAAG 1 cut(s) 1663
BspTNI GGTCTC 1 cut(s) 1446
BsrFI RCCGGY 2 cut(s) 50, 144
BsrI ACTGG 1 cut(s) 854
BssAI RCCGGY 2 cut(s) 50, 144
BssECI CCNNGG 6 cut(s) 57, 90, 204, 550, 860, 1495
BssMI GATC 5 cut(s) 217, 546, 808, 1252, 1667
BssNAI GTATAC 1 cut(s) 441
BssNI GRCGYC 1 cut(s) 321
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 2 cut(s) 550, 1495
Bst1107I GTATAC 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 3 cut(s) 861, 1190, 1419
Bst4CI ACNGT 6 cut(s) 580, 950, 1023, 1404, 1462, 1786
Bst6I CTCTTC 2 cut(s) 1407, 1463
BstACI GRCGYC 1 cut(s) 321
BstAFI CTTAAG 1 cut(s) 1663
BstAPI GCANNNNNTGC 1 cut(s) 1366
BstBAI YACGTR 1 cut(s) 927
BstC8I GCNNGC 5 cut(s) 52, 211, 557, 1228, 1283
BstDEI CTNAG 1 cut(s) 1047
BstDSI CCRYGG 3 cut(s) 90, 204, 550
BstF5I GGATG 4 cut(s) 165, 739, 1198, 1545
BstH2I RGCGCY 2 cut(s) 151, 252
BstHHI GCGC 3 cut(s) 150, 251, 520
BstKTI GATC 5 cut(s) 220, 549, 811, 1255, 1670
BstMAI GTCTC 7 cut(s) 349, 1331, 1410, 1446, 1569, 1625, 1630
BstMBI GATC 5 cut(s) 217, 546, 808, 1252, 1667
BstMWI GCNNNNNNNGC 5 cut(s) 60, 111, 147, 561, 1366
BstNI CCWGG 3 cut(s) 861, 1190, 1419
BstNSI RCATGY 1 cut(s) 559
BstSCI CCNGG 5 cut(s) 213, 292, 859, 1188, 1417
BstSNI TACGTA 1 cut(s) 927
BstV1I GCAGC 4 cut(s) 50, 532, 614, 736
BstV2I GAAGAC 1 cut(s) 1455
BstX2I RGATCY 3 cut(s) 217, 546, 1667
BstYI RGATCY 3 cut(s) 217, 546, 1667
BstZ17I GTATAC 1 cut(s) 441
Bsu15I ATCGAT 1 cut(s) 1355
BsuI GTATCC 1 cut(s) 1668
BsuRI GGCC 8 cut(s) 50, 54, 79, 95, 105, 669, 1188, 1611
BsuTUI ATCGAT 1 cut(s) 1355
BtgI CCRYGG 3 cut(s) 90, 204, 550
BtgZI GCGATG 1 cut(s) 102
BtrI CACGTC 2 cut(s) 331, 373
BtsCI GGATG 4 cut(s) 165, 739, 1198, 1545
BtsI GCAGTG 1 cut(s) 959
BtsIMutI CAGTG 4 cut(s) 959, 1101, 1458, 1782
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 5 cut(s) 52, 211, 557, 1228, 1283
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 1 cut(s) 1444
CfoI GCGC 3 cut(s) 150, 251, 520
Cfr10I RCCGGY 2 cut(s) 50, 144
Cfr13I GGNCC 4 cut(s) 53, 154, 857, 1597
ClaI ATCGAT 1 cut(s) 1355
CseI GACGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 928
CviAII CATG 6 cut(s) 551, 556, 911, 1361, 1445, 1501
CviQI GTAC 1 cut(s) 928
DdeI CTNAG 1 cut(s) 1047
DpnI GATC 5 cut(s) 219, 548, 810, 1254, 1669
DpnII GATC 5 cut(s) 217, 546, 808, 1252, 1667
EaeI YGGCCR 2 cut(s) 77, 93
Eam1104I CTCTTC 2 cut(s) 1407, 1463
EarI CTCTTC 2 cut(s) 1407, 1463
EciI GGCGGA 1 cut(s) 183
Eco105I TACGTA 1 cut(s) 927
Eco130I CCWWGG 2 cut(s) 550, 1495
Eco147I AGGCCT 1 cut(s) 1611
Eco24I GRGCYC 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 1446
Eco47I GGWCC 3 cut(s) 154, 857, 1597
Eco47III AGCGCT 1 cut(s) 250
Eco57I CTGAAG 3 cut(s) 1431, 1487, 1626
Eco88I CYCGRG 2 cut(s) 56, 585
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 3 cut(s) 859, 1188, 1417
EcoT14I CCWWGG 2 cut(s) 550, 1495
EcoT22I ATGCAT 1 cut(s) 1362
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 2 cut(s) 550, 1495
Esp3I CGTCTC 1 cut(s) 349
FaeI CATG 6 cut(s) 554, 559, 914, 1364, 1448, 1504
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FaqI GGGAC 1 cut(s) 466
FatI CATG 6 cut(s) 550, 555, 910, 1360, 1444, 1500
FauI CCCGC 2 cut(s) 491, 552
FblI GTMKAC 1 cut(s) 440
Fnu4HI GCNGC 7 cut(s) 64, 80, 103, 197, 521, 628, 750
FokI GGATG 4 cut(s) 152, 746, 1205, 1552
FriOI GRGCYC 1 cut(s) 129
FseI GGCCGGCC 1 cut(s) 54
Fsp4HI GCNGC 7 cut(s) 64, 80, 103, 197, 521, 628, 750
FspBI CTAG 2 cut(s) 246, 1271
FspI TGCGCA 1 cut(s) 519
GlaI GCGC 3 cut(s) 149, 250, 519
GluI GCNGC 7 cut(s) 64, 80, 103, 197, 521, 628, 750
GsaI CCCAGC 2 cut(s) 154, 1387
HaeII RGCGCY 2 cut(s) 151, 252
HaeIII GGCC 8 cut(s) 50, 54, 79, 95, 105, 669, 1188, 1611
HapII CCGG 5 cut(s) 51, 122, 145, 215, 294
HgaI GACGC 1 cut(s) 329
HhaI GCGC 3 cut(s) 150, 251, 520
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 6 cut(s) 554, 559, 914, 1364, 1448, 1504
Hin6I GCGC 3 cut(s) 148, 249, 518
HinP1I GCGC 3 cut(s) 148, 249, 518
HincII GTYRAC 2 cut(s) 457, 1515
HindII GTYRAC 2 cut(s) 457, 1515
HinfI GANTC 5 cut(s) 182, 479, 702, 1487, 1638
HpaII CCGG 5 cut(s) 51, 122, 145, 215, 294
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 6 cut(s) 204, 441, 457, 1400, 1515, 1597
Hpy188I TCNGA 3 cut(s) 255, 319, 1173
Hpy188III TCNNGA 6 cut(s) 699, 706, 1397, 1409, 1445, 1604
Hpy8I GTNNAC 6 cut(s) 204, 441, 457, 1400, 1515, 1597
Hpy99I CGWCG 4 cut(s) 176, 182, 323, 377
HpyAV CCTTC 5 cut(s) 973, 1237, 1601, 1610, 1738
HpyCH4III ACNGT 6 cut(s) 580, 950, 1023, 1404, 1462, 1786
HpyCH4IV ACGT 5 cut(s) 330, 342, 372, 926, 1426
HpyCH4V TGCA 5 cut(s) 964, 1018, 1121, 1281, 1360
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 111, 147, 561, 1366
HpyF3I CTNAG 1 cut(s) 1047
HpySE526I ACGT 5 cut(s) 330, 342, 372, 926, 1426
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 6 cut(s) 554, 559, 914, 1364, 1448, 1504
HspAI GCGC 3 cut(s) 148, 249, 518
KroI GCCGGC 1 cut(s) 50
KroNI GCCGGC 1 cut(s) 52
Kzo9I GATC 5 cut(s) 217, 546, 808, 1252, 1667
LguI GCTCTTC 1 cut(s) 1407
LmnI GCTCC 3 cut(s) 124, 256, 746
Lsp1109I GCAGC 4 cut(s) 50, 532, 614, 736
LweI GCATC 3 cut(s) 910, 1347, 1530
MaeI CTAG 2 cut(s) 246, 1271
MaeII ACGT 5 cut(s) 330, 342, 372, 926, 1426
MaeIII GTNAC 2 cut(s) 816, 1258
MalI GATC 5 cut(s) 219, 548, 810, 1254, 1669
MboI GATC 5 cut(s) 217, 546, 808, 1252, 1667
MboII GAAGA 5 cut(s) 438, 1019, 1424, 1460, 1480
MflI RGATCY 3 cut(s) 217, 546, 1667
MhlI GDGCHC 1 cut(s) 129
MlyI GAGTC 3 cut(s) 176, 488, 1632
MmeI TCCRAC 4 cut(s) 278, 342, 363, 1365
Mph1103I ATGCAT 1 cut(s) 1362
MroNI GCCGGC 1 cut(s) 50
MroXI GAANNNNTTC 3 cut(s) 690, 1392, 1486
MslI CAYNNNNRTG 1 cut(s) 1505
MspCI CTTAAG 1 cut(s) 1663
MspI CCGG 5 cut(s) 51, 122, 145, 215, 294
MspR9I CCNGG 5 cut(s) 215, 294, 861, 1190, 1419
MvaI CCWGG 3 cut(s) 861, 1190, 1419
MwoI GCNNNNNNNGC 5 cut(s) 60, 111, 147, 561, 1366
NaeI GCCGGC 1 cut(s) 52
NciI CCSGG 2 cut(s) 215, 294
NcoI CCATGG 1 cut(s) 550
NdeII GATC 5 cut(s) 217, 546, 808, 1252, 1667
NgoMIV GCCGGC 1 cut(s) 50
NlaIII CATG 6 cut(s) 554, 559, 914, 1364, 1448, 1504
NlaIV GGNNCC 4 cut(s) 55, 126, 155, 548
NmuCI GTSAC 2 cut(s) 816, 1258
NsbI TGCGCA 1 cut(s) 519
NsiI ATGCAT 1 cut(s) 1362
NspI RCATGY 1 cut(s) 559
PaeI GCATGC 1 cut(s) 559
PaeR7I CTCGAG 1 cut(s) 585
PagI TCATGA 1 cut(s) 1444
PaqCI CACCTGC 1 cut(s) 362
PceI AGGCCT 1 cut(s) 1611
PciSI GCTCTTC 1 cut(s) 1407
PcsI WCGNNNNNNNCGW 3 cut(s) 315, 327, 369
PdiI GCCGGC 1 cut(s) 52
PdmI GAANNNNTTC 3 cut(s) 690, 1392, 1486
PfeI GAWTC 2 cut(s) 702, 1487
PflFI GACNNNGTC 2 cut(s) 308, 329
PflMI CCANNNNNTGG 1 cut(s) 1501
PkrI GCNGC 7 cut(s) 65, 81, 104, 198, 522, 629, 751
PleI GAGTC 3 cut(s) 176, 487, 1632
PpsI GAGTC 3 cut(s) 176, 487, 1632
Ppu21I YACGTR 1 cut(s) 927
PpuMI RGGWCCY 1 cut(s) 154
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 3 cut(s) 859, 1188, 1417
PspFI CCCAGC 2 cut(s) 150, 1383
PspGI CCWGG 3 cut(s) 859, 1188, 1417
PspN4I GGNNCC 4 cut(s) 55, 126, 155, 548
PspPI GGNCC 4 cut(s) 53, 154, 857, 1597
PspPPI RGGWCCY 1 cut(s) 154
PspXI VCTCGAGB 1 cut(s) 585
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 3 cut(s) 217, 546, 1667
PsyI GACNNNGTC 2 cut(s) 308, 329
RigI GGCCGGCC 1 cut(s) 54
RsaI GTAC 1 cut(s) 929
RsaNI GTAC 1 cut(s) 928
RseI CAYNNNNRTG 1 cut(s) 1505
SapI GCTCTTC 1 cut(s) 1407
SatI GCNGC 7 cut(s) 64, 80, 103, 197, 521, 628, 750
Sau3AI GATC 5 cut(s) 217, 546, 808, 1252, 1667
Sau96I GGNCC 4 cut(s) 53, 154, 857, 1597
SchI GAGTC 3 cut(s) 176, 488, 1632
ScrFI CCNGG 5 cut(s) 215, 294, 861, 1190, 1419
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 3 cut(s) 910, 1347, 1530
Sfr274I CTCGAG 1 cut(s) 585
SgrAI CRCCGGYG 1 cut(s) 144
SinI GGWCC 3 cut(s) 154, 857, 1597
SlaI CTCGAG 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 1505
SmlI CTYRAG 4 cut(s) 70, 585, 1633, 1663
SmoI CTYRAG 4 cut(s) 70, 585, 1633, 1663
SnaBI TACGTA 1 cut(s) 927
SphI GCATGC 1 cut(s) 559
SseBI AGGCCT 1 cut(s) 1611
SsiI CCGC 7 cut(s) 80, 102, 194, 197, 484, 559, 766
SspI AATATT 1 cut(s) 1212
SspMI CTAG 2 cut(s) 246, 1271
StuI AGGCCT 1 cut(s) 1611
StyD4I CCNGG 5 cut(s) 213, 292, 859, 1188, 1417
StyI CCWWGG 2 cut(s) 550, 1495
TaaI ACNGT 6 cut(s) 580, 950, 1023, 1404, 1462, 1786
TaiI ACGT 5 cut(s) 333, 345, 375, 929, 1429
TaqI TCGA 8 cut(s) 228, 260, 303, 586, 1255, 1355, 1644, 1790
TauI GCSGC 3 cut(s) 82, 105, 199
TfiI GAWTC 2 cut(s) 702, 1487
TscAI CASTG 4 cut(s) 966, 1108, 1465, 1789
TseFI GTSAC 2 cut(s) 816, 1258
TseI GCWGC 4 cut(s) 63, 520, 627, 749
Tsp45I GTSAC 2 cut(s) 816, 1258
TspDTI ATGAA 8 cut(s) 615, 750, 969, 1122, 1209, 1461, 1577, 1710
TspGWI ACGGA 2 cut(s) 424, 1047
TspRI CASTG 4 cut(s) 966, 1108, 1465, 1789
Tth111I GACNNNGTC 2 cut(s) 308, 329
Van91I CCANNNNNTGG 1 cut(s) 1501
Vha464I CTTAAG 1 cut(s) 1663
VpaK11BI GGWCC 3 cut(s) 154, 857, 1597
XapI RAATTY 4 cut(s) 603, 1558, 1682, 1805
XceI RCATGY 1 cut(s) 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XhoI CTCGAG 1 cut(s) 585
XmiI GTMKAC 1 cut(s) 440
XmnI GAANNNNTTC 3 cut(s) 690, 1392, 1486
XspI CTAG 2 cut(s) 246, 1271
Zsp2I ATGCAT 1 cut(s) 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.