Rw2G024080

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000105
Physical Location & Seq
Forward (+)
35471294 .. 35475624
4331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024080.1

Sequence Viewer

Length: 3318 bp
ATGACTACCCAAACAGACATTCCTTCTTCTTCTGCTCCTCTCCGTCCGATCCCTAAATACGATGTCTTCCTTAGTTCCAGTGGAGAAGGCCCCCTTCCAAATTTTACAGATCATCTCTGTACAGCTCTGGAACAGAAGGAGCTATTGACATTTAGAGATGGTGAAGAACGCGAGAGGGGAAAAGCAATTGAAGAATCAAGATGTGCAATTGTGGTTCTTTCAGCCAAGTACACCAATCCATGGTGTTTGGATGCAATTGCAAAGATCGTTGAATGCAAGGAAGTGATGAAACTGACAGTAGTCCCAGTCTTTGATGGTGTGGATCCATCTGATGTACGAAATCAAACAGGGCATCATTTCGGCGAAGCCTTCTCCAAGCATGAAAAGGCCTTTAAAGACGAACCGGAGAAGGTGAGCAGGTGGAGACGTGCTCTTTTACAAGTAGCCGAACTCTCTGGATGGGATTTGCAAAATGTGCAAAATGGTTATGAAGCCAAGATTATTCAAAAAATTACAGAAGACCTTTTTATCAAATTAAACCGTAGGTTACCTGACAGATTAGTTGGGATGGATTCTCACCTGAACAAAATGCTCTCCCGCTTGGAAGTGGGGGGTCCTGGAGTTCGTACCGTAGGGATTTGGGGGATGGGGGGTATCGGAAAGTCAACTATTGCAAGAGCTGTTTTTGACTGGATACATTCTGCGTTTGAAGAACGAGCTTTTCTTTATAATGTTAGAGAACAATTTCAACGTCGACGATTTGATCTACAAGAGGAACTTTGGTGGATGCTTAACCGTAATGTATATGTATCTGACAAGGATATGGGAATAGATATGATCAGACAAAGACTACGTACTACAATGGTTCTTATCGTTCTTGATGATGTGGATAAACTAGAACAATTGGAAGCATTGTGTGATCGTAGTTGGTTTGGGCCAGGGAGTCGAATCATCATAACATCAAGAGACGAGCATTTGCTGAGAGAATTTGGAGTAGATAGCATATACACGGTTGAAAGATTAACTGATGTTGAATCTCTTCAGCTGTTTAGTCAGTATGCCTTCAGGGGTTGTCAAGCAGGAGAATATCCTCAGACGCTTTCTAAGGAAATTGTGAAATATGCTGCTGGCCTTCCATTAGCTCTCAAAGTTTTGGGTGCCAGTCTACGTGGTAGAAGTATAGAACAATGGTCAGCTTCCTTGGATGGACTAAAACACAAACCTAATCGACTTACTGCTGATGTGCTTAGATTAAGTTATGATGGACTAGAAGCATCAACAAAAGTTACTTTTTTGGACGTTGCATGCTTCTTTAAAGGAGAGAATATAGATCGTGTAAAAAGAATACTGCATGGATTGGACAATTGTATTTCAGACATTGATATAGAAGTTCTAGTTGCGAGAGCTCTAGTAACTGTAGATGTGACTGTGGATGGAAGAAAATTATGGATGCATGACCTGCTACAACAATTGGGTCAACAAATTGTTTCTCAAGAATGTTTTGAGCCTGGCAAGCGTAGTCGGTTGTGGATCCCAAAAGATATCATCCATGTACTTGAAAAGAATACGGGTTCAAGTTCACTTGAAGGCATAGCCTTAGACTTTCCAAAAATAGAAGAGGTACATTGCAGCTCAGAGGCATTCTCTAAGATGGACAGACTTCGGATTCTCCAAATCCGAAATGTGCAAATGAAGGAAGGCCCTAGATATCTTTCTCAGAAGTTAATGCTTCTGGAATGGAGTGAATATCCTACAGAAAGTCTCCCACAAAATTTCCTTCCTGATAGACTATGTGAACTTAACCTCTGCCATAGCATGATCAAACGACTTTGGACTGGAAGAAAGGACATGCCCTGCTTGACAGACATCAATTTAAGCTACTCTCAGAAGTTGACCAGGACCCCAGATTTTACATGTGCTCCACGGCTTGAAAGATTAATTCTTGTAGAGTGTAGAAAATTGGTGGTGATCGACCCATCCATTGGTTTTCTTAAAATGCTCACTTGCTTGAATCTTAAAGATTGCACAAGTCTTAAAAGTCTTCCAGATAAGCTTGAAACGAAATGCCTTCGAATTCTTGTTCTTTCAGGCTGTTCAAACGTCAAGAAAATTCCAGATTTTGTGGGATCTATGGAAGGCTTGTCAGAACTTTATTTGGATGAGACTGCTATAGAGCAATTGCCTATATCAGTTCAACTTCTGACTGGCCTCATCCTATTAAATTTGAGAGATTGCAAAAATCTTAAGAGTCTTCCAAGCGACATTGGCAATTTGATGTCCCTCAAGCTTCTCAATATCTGTGGATGCTCAAAACTTGAAGCACTACCAGACAGCTTGGGAAAGATAGACAGTTTGGAAGAGCTTGATGCGAGTGGTACGTCCATTAGTGCCGTACCATCATCCATTTCTCTTCTGGGAAATCTTAAAGTTTTATCTCTTTGTGGATGTAAAGGGATGCCAGGCAAGAGGCCAAGATATCCGCGCAGTTTTCGATTGCCCATTACTGATTCAGGTGGTCTGCGTTCTTTAACCGAACTGAATCTTAGTGACTGTAATCTTCAGGAGGGAACAATTCCAAAAGATATTGGCTGCTTATCGTCATTGGTATCACTAAATGTAAGCAAAAACAGTTTTGTTAGTCTTCCCAAAAGCATTAGACAGCTTTCAAAGCTTCAGAAGTTGAATTTGGAAAGTTGCAAGAGTCTTCAAAATTTGCCGGACCTTTCATCAAAAATAGCCTGTAGTGCTGGGGCAGAAGACGGTAATTCAGAGAAAAGACTGTCATCATGTTTCAATTTGATTAATTGCTTCGAATTGGTTGCCAATGAGAGATGCACAAATATAGCGCTTGCAATGCTAAGGAGATCTCTTCAGGGAATTTCTTATGCAGGAAATAGTTTTGAAACTATAATTCCTGGCAGTGAAATGTCTGAGCTGTTCAGTGATTGGAGTATGGGACCAGCAGTAAGCATGGAACTACCTCGACAATGGCACAAGAATGAGTGGATGGGATATGCATTTTGTGCTTGTTTTGTTATCCGTCGAGATCTTCCACACTGCACATGTCTTCGATGCGAAGTGAATTCACTGGCTGACTGGTGTCCTTCCTTTGCCTGCAGCCAAGAATTAGGCCAAATTGAGTCGGAACACCTTTGGCTATCCTTTGTCTCTGGTGATTATTTTGGAACAGCATGGCAAGAAACCTGCCATAGCATTGAGTTTACATTTAAAACATTAGGCACTGGTATGGAGGTGAAGAAGTGTGGAGTCCGTTTGATATACGAGCACGATTTAGATTTGATTGGAGTGAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1105

Amino Acids

124.42

Weight (kDa)

6.38

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 183 8.6e-33 TIR domain
NB-ARC PF00931 192 - 356 5.3e-18 NB-ARC domain
WHD_ROQ1 PF23282 427 - 497 3.4e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 656 - 767 1.4e-08 Leucine-rich repeat region
LRR_13 PF23286 690 - 772 1e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 740 - 820 1.3e-09 Leucine-rich repeat region
LRR_14 PF23598 839 - 906 2.7e-06 Leucine-rich repeat region
C-JID PF20160 972 - 1098 5.6e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AarI CACCTGC 1 cut(s) 408
Acc36I ACCTGC 3 cut(s) 408, 1467, 3212
AccB1I GGYRCC 1 cut(s) 1157
AccB7I CCANNNNNTGG 2 cut(s) 240, 1982
AccI GTMKAC 2 cut(s) 754, 1165
AccII CGCG 2 cut(s) 171, 2482
AciI CCGC 2 cut(s) 598, 2480
AclWI GGATC 6 cut(s) 43, 317, 330, 1525, 1538, 2134
AcuI CTGAAG 5 cut(s) 1025, 1048, 2543, 2657, 2855
AfaI GTAC 9 cut(s) 121, 230, 336, 628, 856, 1554, 1623, 2377, 2394
AfeI AGCGCT 1 cut(s) 2847
AfiI CCNNNNNNNGG 2 cut(s) 240, 1982
AflII CTTAAG 1 cut(s) 2243
AflIII ACRYGT 2 cut(s) 1913, 3062
AjiI CACGTC 1 cut(s) 428
AjnI CCWGG 6 cut(s) 616, 937, 1507, 1895, 2458, 2914
AjuI GAANNNNNNNTTGG 3 cut(s) 35, 2669, 2701
Alw21I GWGCWC 4 cut(s) 433, 1408, 1921, 3288
Alw26I GTCTC 5 cut(s) 418, 960, 1766, 2156, 3172
AlwI GGATC 6 cut(s) 43, 317, 330, 1525, 1538, 2134
Aor51HI AGCGCT 1 cut(s) 2847
AoxI GGCC 8 cut(s) 88, 387, 935, 1129, 1699, 2206, 2468, 3130
ApeKI GCWGC 4 cut(s) 1124, 1629, 2589, 3117
AseI ATTAAT 2 cut(s) 1937, 2802
Asp700I GAANNNNTTC 2 cut(s) 744, 1038
AspLEI GCGC 2 cut(s) 2484, 2848
AspS9I GGNCC 7 cut(s) 89, 614, 935, 1700, 1899, 2719, 2957
AsuHPI GGTGA 6 cut(s) 173, 424, 569, 1978, 3185, 3265
AsuII TTCGAA 2 cut(s) 2071, 2811
AvaII GGWCC 4 cut(s) 614, 1899, 2719, 2957
BamHI GGATCC 2 cut(s) 322, 1530
BanI GGYRCC 1 cut(s) 1157
BanII GRGCYC 1 cut(s) 1408
BbsI GAAGAC 8 cut(s) 58, 525, 2033, 2243, 2633, 2696, 2763, 3059
Bbv12I GWGCWC 4 cut(s) 433, 1408, 1921, 3288
BbvI GCAGC 4 cut(s) 1111, 1641, 2576, 3129
BceAI ACGGC 2 cut(s) 1940, 2375
BcgI CGANNNNNNTGC 2 cut(s) 2801, 2835
BciT130I CCWGG 6 cut(s) 618, 939, 1509, 1897, 2460, 2916
BciVI GTATCC 1 cut(s) 687
BclI TGATCA 2 cut(s) 837, 1818
BcoDI GTCTC 5 cut(s) 418, 960, 1766, 2156, 3172
BfaI CTAG 5 cut(s) 896, 1268, 1394, 1409, 1704
BfmI CTRYAG 5 cut(s) 1416, 1752, 2169, 2740, 3115
BfoI RGCGCY 1 cut(s) 2849
BfrI CTTAAG 1 cut(s) 2243
BfuAI ACCTGC 3 cut(s) 408, 1467, 3212
BfuI GTATCC 1 cut(s) 687
BglII AGATCT 2 cut(s) 2864, 3046
BisI GCNGC 4 cut(s) 1125, 1630, 2590, 3118
BlsI GCNGC 4 cut(s) 1126, 1631, 2591, 3119
Bme1390I CCNGG 6 cut(s) 618, 939, 1509, 1897, 2460, 2916
Bme18I GGWCC 4 cut(s) 614, 1899, 2719, 2957
BmgBI CACGTC 1 cut(s) 428
BmgT120I GGNCC 7 cut(s) 89, 614, 935, 1700, 1899, 2719, 2957
BmiI GGNNCC 7 cut(s) 91, 324, 615, 1159, 1532, 1901, 2958
BmrFI CCNGG 6 cut(s) 618, 939, 1509, 1897, 2460, 2916
BmrI ACTGGG 1 cut(s) 299
BmuI ACTGGG 1 cut(s) 299
BoxI GACNNNNGTC 2 cut(s) 299, 3099
BpiI GAAGAC 8 cut(s) 58, 525, 2033, 2243, 2633, 2696, 2763, 3059
BplI GAGNNNNNCTC 4 cut(s) 415, 447, 1628, 1660
BpmI CTGGAG 1 cut(s) 639
Bpu10I CCTNAGC 1 cut(s) 2858
Bpu14I TTCGAA 2 cut(s) 2071, 2811
BpuEI CTTGAG 2 cut(s) 1476, 2267
BsaAI YACGTR 2 cut(s) 854, 1169
BsaJI CCNNGG 4 cut(s) 239, 938, 1200, 1922
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 1903, 1933
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 1982
Bse1I ACTGG 9 cut(s) 78, 305, 695, 1161, 1840, 2209, 3093, 3101, 3247
Bse3DI GCAATG 2 cut(s) 1624, 2859
BseBI CCWGG 6 cut(s) 618, 939, 1509, 1897, 2460, 2916
BseDI CCNNGG 4 cut(s) 239, 938, 1200, 1922
BseLI CCNNNNNNNGG 2 cut(s) 240, 1982
BseMI GCAATG 2 cut(s) 1624, 2859
BseMII CTCAG 6 cut(s) 971, 1106, 1647, 1730, 1898, 2922
BseNI ACTGG 9 cut(s) 78, 305, 695, 1161, 1840, 2209, 3093, 3101, 3247
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 4 cut(s) 1111, 1641, 2576, 3129
BseYI CCCAGC 1 cut(s) 2747
BsgI GTGCAG 1 cut(s) 3043
Bsh1236I CGCG 2 cut(s) 171, 2482
BshFI GGCC 8 cut(s) 90, 389, 937, 1131, 1701, 2208, 2470, 3132
BshNI GGYRCC 1 cut(s) 1157
BsiHKAI GWGCWC 4 cut(s) 433, 1408, 1921, 3288
BsiSI CCGG 2 cut(s) 404, 2717
BslFI GGGAC 3 cut(s) 287, 2263, 2970
BslI CCNNNNNNNGG 2 cut(s) 240, 1982
BsmAI GTCTC 5 cut(s) 418, 960, 1766, 2156, 3172
BsmBI CGTCTC 2 cut(s) 418, 960
BsmFI GGGAC 3 cut(s) 287, 2263, 2970
BsmI GAATGC 2 cut(s) 278, 1640
BsnI GGCC 8 cut(s) 90, 389, 937, 1131, 1701, 2208, 2470, 3132
Bsp119I TTCGAA 2 cut(s) 2071, 2811
Bsp1286I GDGCHC 4 cut(s) 433, 1408, 1921, 3288
Bsp1407I TGTACA 1 cut(s) 119
Bsp19I CCATGG 1 cut(s) 239
BspACI CCGC 2 cut(s) 598, 2480
BspANI GGCC 8 cut(s) 90, 389, 937, 1131, 1701, 2208, 2470, 3132
BspCNI CTCAG 6 cut(s) 972, 1105, 1646, 1729, 1897, 2923
BspFNI CGCG 2 cut(s) 171, 2482
BspLI GGNNCC 7 cut(s) 91, 324, 615, 1159, 1532, 1901, 2958
BspMAI CTGCAG 1 cut(s) 3119
BspMI ACCTGC 3 cut(s) 408, 1467, 3212
BspPI GGATC 6 cut(s) 43, 317, 330, 1525, 1538, 2134
BspQI GCTCTTC 1 cut(s) 2352
BspT104I TTCGAA 2 cut(s) 2071, 2811
BspT107I GGYRCC 1 cut(s) 1157
BspTI CTTAAG 1 cut(s) 2243
BsrDI GCAATG 2 cut(s) 1624, 2859
BsrGI TGTACA 1 cut(s) 119
BsrI ACTGG 9 cut(s) 78, 305, 695, 1161, 1840, 2209, 3093, 3101, 3247
BssECI CCNNGG 4 cut(s) 239, 938, 1200, 1922
BssT1I CCWWGG 2 cut(s) 239, 1200
Bst2UI CCWGG 6 cut(s) 618, 939, 1509, 1897, 2460, 2916
Bst6I CTCTTC 5 cut(s) 1044, 1611, 2352, 2415, 2874
BstAFI CTTAAG 1 cut(s) 2243
BstAPI GCANNNNNTGC 3 cut(s) 475, 1459, 3023
BstAUI TGTACA 1 cut(s) 119
BstBAI YACGTR 2 cut(s) 854, 1169
BstBI TTCGAA 2 cut(s) 2071, 2811
BstC8I GCNNGC 5 cut(s) 1129, 1306, 1514, 2850, 3115
BstDSI CCRYGG 2 cut(s) 239, 1922
BstEII GGTNACC 1 cut(s) 546
BstFNI CGCG 2 cut(s) 171, 2482
BstH2I RGCGCY 1 cut(s) 2849
BstHHI GCGC 2 cut(s) 2484, 2848
BstMAI GTCTC 5 cut(s) 418, 960, 1766, 2156, 3172
BstMWI GCNNNNNNNGC 8 cut(s) 475, 1459, 1513, 2265, 2668, 2744, 2854, 3023
BstNI CCWGG 6 cut(s) 618, 939, 1509, 1897, 2460, 2916
BstNSI RCATGY 4 cut(s) 1308, 1852, 1917, 3066
BstPAI GACNNNNGTC 2 cut(s) 299, 3099
BstPI GGTNACC 1 cut(s) 546
BstSCI CCNGG 6 cut(s) 616, 937, 1507, 1895, 2458, 2914
BstSFI CTRYAG 5 cut(s) 1416, 1752, 2169, 2740, 3115
BstSNI TACGTA 1 cut(s) 854
BstUI CGCG 2 cut(s) 171, 2482
BstV1I GCAGC 4 cut(s) 1111, 1641, 2576, 3129
BstV2I GAAGAC 8 cut(s) 58, 525, 2033, 2243, 2633, 2696, 2763, 3059
BstX2I RGATCY 5 cut(s) 322, 1530, 2126, 2864, 3046
BstYI RGATCY 5 cut(s) 322, 1530, 2126, 2864, 3046
BsuI GTATCC 1 cut(s) 687
BsuRI GGCC 8 cut(s) 90, 389, 937, 1131, 1701, 2208, 2470, 3132
BtgI CCRYGG 2 cut(s) 239, 1922
BtrI CACGTC 1 cut(s) 428
BtsI GCAGTG 2 cut(s) 2926, 3055
BtsIMutI CAGTG 6 cut(s) 85, 2926, 2947, 3055, 3086, 3240
BveI ACCTGC 3 cut(s) 408, 1467, 3212
Cac8I GCNNGC 5 cut(s) 1129, 1306, 1514, 2850, 3115
CfoI GCGC 2 cut(s) 2484, 2848
Cfr13I GGNCC 7 cut(s) 89, 614, 935, 1700, 1899, 2719, 2957
CseI GACGC 1 cut(s) 1105
Csp6I GTAC 9 cut(s) 120, 229, 335, 627, 855, 1553, 1622, 2376, 2393
CspCI CAANNNNNGTGG 2 cut(s) 2281, 2316
CviQI GTAC 9 cut(s) 120, 229, 335, 627, 855, 1553, 1622, 2376, 2393
DraI TTTAAA 3 cut(s) 394, 1315, 3229
Eam1104I CTCTTC 5 cut(s) 1044, 1611, 2352, 2415, 2874
EarI CTCTTC 5 cut(s) 1044, 1611, 2352, 2415, 2874
Ecl136II GAGCTC 1 cut(s) 1406
Eco105I TACGTA 1 cut(s) 854
Eco130I CCWWGG 2 cut(s) 239, 1200
Eco147I AGGCCT 1 cut(s) 389
Eco24I GRGCYC 1 cut(s) 1408
Eco32I GATATC 3 cut(s) 1543, 1709, 2477
Eco47I GGWCC 4 cut(s) 614, 1899, 2719, 2957
Eco47III AGCGCT 1 cut(s) 2847
Eco53kI GAGCTC 1 cut(s) 1406
Eco57I CTGAAG 5 cut(s) 1025, 1048, 2543, 2657, 2855
Eco91I GGTNACC 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 1406
EcoO109I RGGNCCY 4 cut(s) 89, 614, 1700, 1899
EcoO65I GGTNACC 1 cut(s) 546
EcoRI GAATTC 2 cut(s) 2073, 3082
EcoRII CCWGG 6 cut(s) 616, 937, 1507, 1895, 2458, 2914
EcoRV GATATC 3 cut(s) 1543, 1709, 2477
EcoT14I CCWWGG 2 cut(s) 239, 1200
EcoT22I ATGCAT 2 cut(s) 1455, 3019
EcoT38I GRGCYC 1 cut(s) 1408
ErhI CCWWGG 2 cut(s) 239, 1200
Esp3I CGTCTC 2 cut(s) 418, 960
FalI AAGNNNNNCTT 4 cut(s) 78, 110, 762, 794
FaqI GGGAC 3 cut(s) 287, 2263, 2970
FauI CCCGC 1 cut(s) 605
FbaI TGATCA 2 cut(s) 837, 1818
FblI GTMKAC 2 cut(s) 754, 1165
Fnu4HI GCNGC 4 cut(s) 1125, 1630, 2590, 3118
FriOI GRGCYC 1 cut(s) 1408
Fsp4HI GCNGC 4 cut(s) 1125, 1630, 2590, 3118
FspBI CTAG 5 cut(s) 896, 1268, 1394, 1409, 1704
GlaI GCGC 2 cut(s) 2483, 2847
GluI GCNGC 4 cut(s) 1125, 1630, 2590, 3118
GsaI CCCAGC 1 cut(s) 2751
GsuI CTGGAG 1 cut(s) 639
HaeII RGCGCY 1 cut(s) 2849
HaeIII GGCC 8 cut(s) 90, 389, 937, 1131, 1701, 2208, 2470, 3132
HapII CCGG 2 cut(s) 404, 2717
HgaI GACGC 1 cut(s) 1105
HhaI GCGC 2 cut(s) 2484, 2848
Hin6I GCGC 2 cut(s) 2482, 2846
HinP1I GCGC 2 cut(s) 2482, 2846
HincII GTYRAC 4 cut(s) 666, 755, 1478, 1893
HindII GTYRAC 4 cut(s) 666, 755, 1478, 1893
HindIII AAGCTT 3 cut(s) 2051, 2285, 2669
HpaII CCGG 2 cut(s) 404, 2717
HphI GGTGA 6 cut(s) 173, 424, 569, 1978, 3185, 3265
Hpy166II GTNNAC 9 cut(s) 231, 666, 755, 1166, 1478, 1580, 1796, 1893, 3222
Hpy8I GTNNAC 9 cut(s) 231, 666, 755, 1166, 1478, 1580, 1796, 1893, 3222
Hpy99I CGWCG 3 cut(s) 756, 759, 3045
HpyCH4IV ACGT 7 cut(s) 427, 751, 853, 1168, 1299, 2100, 2378
HpyF10VI GCNNNNNNNGC 8 cut(s) 475, 1459, 1513, 2265, 2668, 2744, 2854, 3023
HpySE526I ACGT 7 cut(s) 427, 751, 853, 1168, 1299, 2100, 2378
HspAI GCGC 2 cut(s) 2482, 2846
Ksp22I TGATCA 2 cut(s) 837, 1818
LguI GCTCTTC 1 cut(s) 2352
LmnI GCTCC 3 cut(s) 40, 139, 1924
Lsp1109I GCAGC 4 cut(s) 1111, 1641, 2576, 3129
MaeI CTAG 5 cut(s) 896, 1268, 1394, 1409, 1704
MaeII ACGT 7 cut(s) 427, 751, 853, 1168, 1299, 2100, 2378
MaeIII GTNAC 5 cut(s) 546, 1285, 1411, 1423, 2546
MfeI CAATTG 7 cut(s) 186, 207, 255, 902, 1363, 1469, 2177
MflI RGATCY 5 cut(s) 322, 1530, 2126, 2864, 3046
MhlI GDGCHC 4 cut(s) 433, 1408, 1921, 3288
MlyI GAGTC 5 cut(s) 952, 2257, 2710, 3149, 3276
MmeI TCCRAC 1 cut(s) 3123
Mph1103I ATGCAT 2 cut(s) 1455, 3019
MroXI GAANNNNTTC 2 cut(s) 744, 1038
MslI CAYNNNNRTG 2 cut(s) 2997, 3245
MspA1I CMGCKG 1 cut(s) 1045
MspCI CTTAAG 1 cut(s) 2243
MspI CCGG 2 cut(s) 404, 2717
MspR9I CCNGG 6 cut(s) 618, 939, 1509, 1897, 2460, 2916
MunI CAATTG 7 cut(s) 186, 207, 255, 902, 1363, 1469, 2177
Mva1269I GAATGC 2 cut(s) 278, 1640
MvaI CCWGG 6 cut(s) 618, 939, 1509, 1897, 2460, 2916
MvnI CGCG 2 cut(s) 171, 2482
MwoI GCNNNNNNNGC 8 cut(s) 475, 1459, 1513, 2265, 2668, 2744, 2854, 3023
NcoI CCATGG 1 cut(s) 239
NlaIV GGNNCC 7 cut(s) 91, 324, 615, 1159, 1532, 1901, 2958
NmuCI GTSAC 2 cut(s) 1423, 2546
NsiI ATGCAT 2 cut(s) 1455, 3019
NspI RCATGY 4 cut(s) 1308, 1852, 1917, 3066
NspV TTCGAA 2 cut(s) 2071, 2811
PaeI GCATGC 1 cut(s) 1308
PaqCI CACCTGC 1 cut(s) 408
PceI AGGCCT 1 cut(s) 389
PciI ACATGT 2 cut(s) 1913, 3062
PciSI GCTCTTC 1 cut(s) 2352
PctI GAATGC 2 cut(s) 278, 1640
PdmI GAANNNNTTC 2 cut(s) 744, 1038
PfeI GAWTC 8 cut(s) 194, 572, 948, 1034, 1666, 2011, 2507, 2539
PflMI CCANNNNNTGG 2 cut(s) 240, 1982
PfoI TCCNGGA 1 cut(s) 616
PkrI GCNGC 4 cut(s) 1126, 1631, 2591, 3119
PleI GAGTC 5 cut(s) 951, 2256, 2709, 3148, 3275
PpsI GAGTC 5 cut(s) 951, 2256, 2709, 3148, 3275
Ppu21I YACGTR 2 cut(s) 854, 1169
PpuMI RGGWCCY 2 cut(s) 614, 1899
PscI ACATGT 2 cut(s) 1913, 3062
PshAI GACNNNNGTC 2 cut(s) 299, 3099
PshBI ATTAAT 2 cut(s) 1937, 2802
PsiI TTATAA 1 cut(s) 729
Psp124BI GAGCTC 1 cut(s) 1408
Psp5II RGGWCCY 2 cut(s) 614, 1899
Psp6I CCWGG 6 cut(s) 616, 937, 1507, 1895, 2458, 2914
PspEI GGTNACC 1 cut(s) 546
PspFI CCCAGC 1 cut(s) 2747
PspGI CCWGG 6 cut(s) 616, 937, 1507, 1895, 2458, 2914
PspN4I GGNNCC 7 cut(s) 91, 324, 615, 1159, 1532, 1901, 2958
PspPI GGNCC 7 cut(s) 89, 614, 935, 1700, 1899, 2719, 2957
PspPPI RGGWCCY 2 cut(s) 614, 1899
PstI CTGCAG 1 cut(s) 3119
PsuI RGATCY 5 cut(s) 322, 1530, 2126, 2864, 3046
PvuII CAGCTG 1 cut(s) 1045
RsaI GTAC 9 cut(s) 121, 230, 336, 628, 856, 1554, 1623, 2377, 2394
RsaNI GTAC 9 cut(s) 120, 229, 335, 627, 855, 1553, 1622, 2376, 2393
RseI CAYNNNNRTG 2 cut(s) 2997, 3245
SacI GAGCTC 1 cut(s) 1408
SalI GTCGAC 1 cut(s) 753
SapI GCTCTTC 1 cut(s) 2352
SatI GCNGC 4 cut(s) 1125, 1630, 2590, 3118
Sau96I GGNCC 7 cut(s) 89, 614, 935, 1700, 1899, 2719, 2957
SchI GAGTC 5 cut(s) 952, 2257, 2710, 3149, 3276
ScrFI CCNGG 6 cut(s) 618, 939, 1509, 1897, 2460, 2916
SduI GDGCHC 4 cut(s) 433, 1408, 1921, 3288
SfcI CTRYAG 5 cut(s) 1416, 1752, 2169, 2740, 3115
SfuI TTCGAA 2 cut(s) 2071, 2811
SgrDI CGTCGACG 1 cut(s) 753
SinI GGWCC 4 cut(s) 614, 1899, 2719, 2957
SmiMI CAYNNNNRTG 2 cut(s) 2997, 3245
SmlI CTYRAG 3 cut(s) 1491, 2243, 2282
SmoI CTYRAG 3 cut(s) 1491, 2243, 2282
SnaBI TACGTA 1 cut(s) 854
SphI GCATGC 1 cut(s) 1308
SseBI AGGCCT 1 cut(s) 389
SsiI CCGC 2 cut(s) 598, 2480
SspMI CTAG 5 cut(s) 896, 1268, 1394, 1409, 1704
SstI GAGCTC 1 cut(s) 1408
StuI AGGCCT 1 cut(s) 389
StyD4I CCNGG 6 cut(s) 616, 937, 1507, 1895, 2458, 2914
StyI CCWWGG 2 cut(s) 239, 1200
TaiI ACGT 7 cut(s) 430, 754, 856, 1171, 1302, 2103, 2381
TatI WGTACW 3 cut(s) 119, 228, 1552
TfiI GAWTC 8 cut(s) 194, 572, 948, 1034, 1666, 2011, 2507, 2539
TscAI CASTG 6 cut(s) 85, 2926, 2947, 3062, 3093, 3247
TseFI GTSAC 2 cut(s) 1423, 2546
TseI GCWGC 4 cut(s) 1124, 1629, 2589, 3117
Tsp45I GTSAC 2 cut(s) 1423, 2546
TspDTI ATGAA 5 cut(s) 302, 396, 504, 1706, 2715
TspGWI ACGGA 3 cut(s) 32, 3029, 3260
TspRI CASTG 6 cut(s) 85, 2926, 2947, 3062, 3093, 3247
Van91I CCANNNNNTGG 2 cut(s) 240, 1982
Vha464I CTTAAG 1 cut(s) 2243
VpaK11BI GGWCC 4 cut(s) 614, 1899, 2719, 2957
VspI ATTAAT 2 cut(s) 1937, 2802
XceI RCATGY 4 cut(s) 1308, 1852, 1917, 3066
XcmI CCANNNNNNNNNTGG 1 cut(s) 2410
XmiI GTMKAC 2 cut(s) 754, 1165
XmnI GAANNNNTTC 2 cut(s) 744, 1038
XspI CTAG 5 cut(s) 896, 1268, 1394, 1409, 1704
Zsp2I ATGCAT 2 cut(s) 1455, 3019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.