Rmu_sc0001209.1_g000031

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001209.1
OG0000105
Physical Location & Seq
Reverse (-)
129094 .. 132172
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001209.1_g000031.1.cds

Sequence Viewer

Length: 2754 bp
atgatagaaaggcggttatgcaaactaaaggtgcttgttcttattgatgatgtgaatcacatcaatcaattagataaattggctggaagtcgggactggttcggtcctgggagtagaattgttgtaaccaccccagatattcatttgctaaggggacatgatgttgatgctacatacaagcctactgggttaagtgatggtgaagctattcaactattgagtttaaaggctttcaagaaaagcttcccaccagaagattatttggacttgtgccaccatattatagggtatgctcaggggcttccattggctcttgtggttttaggttcttttctatttggtagaagacccaatgaatgggaaagtgcaatagagaggctaaacaataccccaaatagacaagttatggatgtgcttcagattagttttgatggactagaggaaaaagaccaacagatattcctacatattgcctgtttgtacaaggggaaggacagggatcgtgtgacacaaatactaggctattgcaagctagaacctggcattggtttaagagttcttgaggaaaaatctctcattacaatttttaacaacaaactgtcgatgcatgatttgctacaagaaatgggcttggaaatagttcgtcgagagtcccctgatgagccaggtaaacgaagtagattatggtctcctgaagatatccacaaagtgctgaagaaaaataagggaacagacaaaatccgagccatggtaatggacttgcctgagttagaagtggctcattggaaaccagaagccttttcaaatttgtctcaactcagtcttctccatattcgtaacgtggatcttcccaaaggcctcacttttttatctaattcattgaggctccttgattggagcgggtaccccttaagatccctcccacaaaattttgaaccagacgaacttattgaacttaacttgtgccacagcaacattgaacacctctgggaaggagcaaagaattttgacaagttaaagttcatcaaactatgccattctcaaaaaattgtccagaccccagacctcacaggcgttcaaaatcttgagactttagatcttgaaggatgtaagactttggtaaaacttcatcaatctgtcggacaactcaaaaggcttattgtattgaatcttaaagattgcgagagtctcgagaatctgccaagtaagattgaaatggaatctcttgaaactttaattctttctaactgctccaaagttaaaaagattcccgagttcgctggaaatatgaagcgtttatcaaagctttatctcaatgagactgccattgaaaaactacctgtgtcaattggatgcctgagtggccttgcttcactgaatcttagcaactgcaaaaatcttctatgccttgcaagcaccttagaactgcttagtagattggagtctctgaaaaagcttgatctttctggatgtttgaaacttcgagaacaggatagtgtgagggagaatggtgcaattagaactgctgtaaatcatcttataaaacgtggttgtgaagttgggaaatatttgtcatggtccttgccttctagattggtgcaaagagcctatatggagccaatgagtatgaagttgcctgtgtctgatctgtgttattctacagagcaaaatgcaaatacatcaatgagtatccggttgccactatcttgtctatggaatttaacagatctaaacttaagtaactgcaatcttggtgacactgcatttgccaactttgcctgttttccctctttagtggcactaaatctgagtggaaataattttgttaaccttcctccaagcatcaggtcctgttttaagcttcagaacattaatttggagaattgcaagacacttgaagagttgtccggacttccatcaaatagtaaattggatgtgagggcagatggttgttcttcactggaaatgctgtttgatgtatccaagttcaatagattggagaaatcatatttcaatttcatcaattgcttcaaactaaatgacaatcagggctgctgtaacatagcatttgaaatgctgaggatattccttaatcagcaagtttctagtgtgacggaaacttttcaaattgtaattcctggaagtggaattcctgagtggtttgatcatcgaagatttgggagttcattaactgtagacctacttccagattggaatgagagtaggtttttgggatttgttttgtgtgctgtttttgtactccatgagcaccgtcaggttgatgaccttgctatccatcagttcaagacttttaaggccactcatcatcttgtatgttgcctgaagctcaatggaagagaattagaagtatacggcaaacagcctgcatttcgctttagcgaacaattttgccaggttgagtcagatcacctttggatattctatgtgtcccgtgataaatactttggtacagattggtggcataacagttgtagtcagcttgagttcttatttgaaacaagagggccaggcctgagggtgaaggagtgtggggtacgtctgatatatgagcaagatgtgctagagttgaaccaaacaataactcaatcaagcagtaggatgtctcctccatatgaggatatattgactgattttgagaatcaagttggaggggaaaccattcacccaaaaaaaaagttgaaaggggaaacttgtggtactggtagtagaacctgcacccttgaagaactatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

917

Amino Acids

104.25

Weight (kDa)

6.42

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1541
Acc36I ACCTGC 1 cut(s) 2741
Acc65I GGTACC 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 903
AccB7I CCANNNNNTGG 1 cut(s) 357
AccBSI CCGCTC 1 cut(s) 900
AccI GTMKAC 2 cut(s) 2196, 2370
AccIII TCCGGA 1 cut(s) 1907
AciI CCGC 2 cut(s) 13, 900
AclWI GGATC 3 cut(s) 507, 852, 909
AcsI RAATTY 5 cut(s) 805, 928, 1003, 1717, 2148
AcuI CTGAAG 5 cut(s) 401, 714, 734, 1847, 2363
AdeI CACNNNGTG 1 cut(s) 709
AfaI GTAC 6 cut(s) 482, 905, 2259, 2470, 2556, 2719
AfiI CCNNNNNNNGG 4 cut(s) 357, 545, 748, 2144
AflII CTTAAG 2 cut(s) 910, 1735
AjnI CCWGG 6 cut(s) 106, 538, 664, 2137, 2412, 2527
AjuI GAANNNNNNNTTGG 4 cut(s) 528, 560, 1859, 1891
AluBI AGCT 8 cut(s) 206, 243, 532, 1306, 1456, 1861, 2347, 2500
AluI AGCT 8 cut(s) 206, 243, 532, 1306, 1456, 1861, 2347, 2500
Alw21I GWGCWC 1 cut(s) 2271
Alw26I GTCTC 7 cut(s) 693, 816, 1082, 1193, 1313, 1449, 2628
AlwI GGATC 3 cut(s) 507, 852, 909
AlwNI CAGNNNCTG 1 cut(s) 1851
Ama87I CYCGRG 2 cut(s) 1190, 1271
Aor13HI TCCGGA 1 cut(s) 1907
AoxI GGCC 5 cut(s) 856, 1363, 2316, 2525, 2530
ApeKI GCWGC 1 cut(s) 2052
ApoI RAATTY 5 cut(s) 805, 928, 1003, 1717, 2148
ArsI GACNNNNNNTTYG 2 cut(s) 1748, 1780
AseI ATTAAT 1 cut(s) 1872
Asp700I GAANNNNTTC 5 cut(s) 207, 242, 639, 2121, 2679
Asp718I GGTACC 1 cut(s) 903
AspS9I GGNCC 4 cut(s) 104, 1578, 1848, 2525
AsuHPI GGTGA 5 cut(s) 212, 1766, 2420, 2551, 2675
AvaI CYCGRG 2 cut(s) 1190, 1271
AvaII GGWCC 3 cut(s) 104, 1578, 1848
AxyI CCTNAGG 1 cut(s) 2534
BanI GGYRCC 1 cut(s) 903
BarI GAAGNNNNNNTAC 2 cut(s) 2187, 2219
BbsI GAAGAC 2 cut(s) 352, 815
Bbv12I GWGCWC 1 cut(s) 2271
BbvCI CCTCAGC 1 cut(s) 2078
BbvI GCAGC 1 cut(s) 2039
BccI CCATC 5 cut(s) 191, 425, 1924, 1940, 2304
BceAI ACGGC 1 cut(s) 2389
BciT130I CCWGG 6 cut(s) 108, 540, 666, 2139, 2414, 2529
BciVI GTATCC 2 cut(s) 1700, 1990
BclI TGATCA 1 cut(s) 2164
BcoDI GTCTC 7 cut(s) 693, 816, 1082, 1193, 1313, 1449, 2628
BfaI CTAG 6 cut(s) 437, 518, 533, 1590, 2106, 2582
BfmI CTRYAG 3 cut(s) 1659, 2193, 2750
BfrI CTTAAG 2 cut(s) 910, 1735
BfuAI ACCTGC 1 cut(s) 2741
BfuI GTATCC 2 cut(s) 1700, 1990
BglI GCCNNNNNGGC 1 cut(s) 1362
BglII AGATCT 2 cut(s) 1096, 1726
BisI GCNGC 1 cut(s) 2053
BlsI GCNGC 1 cut(s) 2054
Bme1390I CCNGG 6 cut(s) 108, 540, 666, 2139, 2414, 2529
Bme18I GGWCC 3 cut(s) 104, 1578, 1848
BmeT110I CYCGRG 2 cut(s) 1190, 1271
BmgT120I GGNCC 4 cut(s) 104, 1578, 1848, 2525
BmiI GGNNCC 3 cut(s) 887, 905, 1617
BmrFI CCNGG 6 cut(s) 108, 540, 666, 2139, 2414, 2529
BmrI ACTGGG 1 cut(s) 195
BmsI GCATC 4 cut(s) 157, 594, 1343, 1851
BmuI ACTGGG 1 cut(s) 195
BpiI GAAGAC 2 cut(s) 352, 815
Bpu10I CCTNAGC 3 cut(s) 149, 294, 2078
BpuEI CTTGAG 3 cut(s) 581, 1106, 2522
BsaBI GATNNNNATC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 693
BsaJI CCNNGG 2 cut(s) 107, 747
BsaWI WCCGGW 2 cut(s) 1692, 1907
Bsc4I CCNNNNNNNGG 4 cut(s) 357, 545, 748, 2144
Bse1I ACTGG 4 cut(s) 101, 190, 1965, 2725
Bse21I CCTNAGG 1 cut(s) 2534
Bse8I GATNNNNATC 1 cut(s) 54
BseAI TCCGGA 1 cut(s) 1907
BseBI CCWGG 6 cut(s) 108, 540, 666, 2139, 2414, 2529
BseDI CCNNGG 2 cut(s) 107, 747
BseGI GGATG 6 cut(s) 415, 1112, 1358, 1475, 1939, 2625
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 4 cut(s) 357, 545, 748, 2144
BseMII CTCAG 8 cut(s) 308, 756, 832, 1349, 1799, 2069, 2145, 2525
BseNI ACTGG 4 cut(s) 101, 190, 1965, 2725
BseRI GAGGAG 1 cut(s) 2616
BseXI GCAGC 1 cut(s) 2039
BsgI GTGCAG 1 cut(s) 2719
BshFI GGCC 5 cut(s) 858, 1365, 2318, 2527, 2532
BshNI GGYRCC 1 cut(s) 903
BsiHKAI GWGCWC 1 cut(s) 2271
BsiHKCI CYCGRG 2 cut(s) 1190, 1271
BsiSI CCGG 2 cut(s) 1693, 1908
BslFI GGGAC 4 cut(s) 107, 168, 637, 2434
BslI CCNNNNNNNGG 4 cut(s) 357, 545, 748, 2144
BsmAI GTCTC 7 cut(s) 693, 816, 1082, 1193, 1313, 1449, 2628
BsmFI GGGAC 4 cut(s) 107, 168, 637, 2434
BsnI GGCC 5 cut(s) 858, 1365, 2318, 2527, 2532
Bso31I GGTCTC 1 cut(s) 693
BsoBI CYCGRG 2 cut(s) 1190, 1271
Bsp1286I GDGCHC 1 cut(s) 2271
Bsp13I TCCGGA 1 cut(s) 1907
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 9 cut(s) 499, 844, 914, 1096, 1459, 1645, 1726, 2164, 2425
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 2 cut(s) 13, 900
BspANI GGCC 5 cut(s) 858, 1365, 2318, 2527, 2532
BspCNI CTCAG 8 cut(s) 307, 757, 831, 1350, 1800, 2070, 2146, 2526
BspEI TCCGGA 1 cut(s) 1907
BspLI GGNNCC 3 cut(s) 887, 905, 1617
BspMI ACCTGC 1 cut(s) 2741
BspPI GGATC 3 cut(s) 507, 852, 909
BspT107I GGYRCC 1 cut(s) 903
BspTI CTTAAG 2 cut(s) 910, 1735
BspTNI GGTCTC 1 cut(s) 693
BsrBI CCGCTC 1 cut(s) 900
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 4 cut(s) 101, 190, 1965, 2725
BssECI CCNNGG 2 cut(s) 107, 747
BssMI GATC 9 cut(s) 499, 844, 914, 1096, 1459, 1645, 1726, 2164, 2425
BssNAI GTATAC 1 cut(s) 2371
BssT1I CCWWGG 1 cut(s) 747
Bst1107I GTATAC 1 cut(s) 2371
Bst2UI CCWGG 6 cut(s) 108, 540, 666, 2139, 2414, 2529
Bst4CI ACNGT 4 cut(s) 600, 2194, 2273, 2489
Bst6I CTCTTC 2 cut(s) 1893, 2350
BstAFI CTTAAG 2 cut(s) 910, 1735
BstAPI GCANNNNNTGC 2 cut(s) 613, 2578
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 3 cut(s) 530, 1414, 2385
BstDSI CCRYGG 1 cut(s) 747
BstF5I GGATG 6 cut(s) 415, 1112, 1358, 1475, 1939, 2625
BstKTI GATC 9 cut(s) 502, 847, 917, 1099, 1462, 1648, 1729, 2167, 2428
BstMAI GTCTC 7 cut(s) 693, 816, 1082, 1193, 1313, 1449, 2628
BstMBI GATC 9 cut(s) 499, 844, 914, 1096, 1459, 1645, 1726, 2164, 2425
BstMWI GCNNNNNNNGC 5 cut(s) 613, 1362, 1413, 1775, 2578
BstNI CCWGG 6 cut(s) 108, 540, 666, 2139, 2414, 2529
BstSCI CCNGG 6 cut(s) 106, 538, 664, 2137, 2412, 2527
BstSFI CTRYAG 3 cut(s) 1659, 2193, 2750
BstV1I GCAGC 1 cut(s) 2039
BstV2I GAAGAC 2 cut(s) 352, 815
BstX2I RGATCY 4 cut(s) 844, 914, 1096, 1726
BstXI CCANNNNNNTGG 1 cut(s) 754
BstYI RGATCY 4 cut(s) 844, 914, 1096, 1726
BstZ17I GTATAC 1 cut(s) 2371
Bsu36I CCTNAGG 1 cut(s) 2534
BsuI GTATCC 2 cut(s) 1700, 1990
BsuRI GGCC 5 cut(s) 858, 1365, 2318, 2527, 2532
BtgI CCRYGG 1 cut(s) 747
BtsCI GGATG 6 cut(s) 415, 1112, 1358, 1475, 1939, 2625
BtsI GCAGTG 1 cut(s) 1758
BtsIMutI CAGTG 3 cut(s) 1373, 1758, 1958
BveI ACCTGC 1 cut(s) 2741
Cac8I GCNNGC 3 cut(s) 530, 1414, 2385
CaiI CAGNNNCTG 1 cut(s) 1851
Cfr13I GGNCC 4 cut(s) 104, 1578, 1848, 2525
Csp6I GTAC 6 cut(s) 481, 904, 2258, 2469, 2555, 2718
CviAII CATG 5 cut(s) 158, 608, 748, 1575, 2264
CviQI GTAC 6 cut(s) 481, 904, 2258, 2469, 2555, 2718
DpnI GATC 9 cut(s) 501, 846, 916, 1098, 1461, 1647, 1728, 2166, 2427
DpnII GATC 9 cut(s) 499, 844, 914, 1096, 1459, 1645, 1726, 2164, 2425
DraI TTTAAA 1 cut(s) 225
DraIII CACNNNGTG 1 cut(s) 709
Eam1104I CTCTTC 2 cut(s) 1893, 2350
EarI CTCTTC 2 cut(s) 1893, 2350
Eco130I CCWWGG 1 cut(s) 747
Eco147I AGGCCT 2 cut(s) 858, 2532
Eco31I GGTCTC 1 cut(s) 693
Eco32I GATATC 1 cut(s) 700
Eco47I GGWCC 3 cut(s) 104, 1578, 1848
Eco57I CTGAAG 5 cut(s) 401, 714, 734, 1847, 2363
Eco81I CCTNAGG 1 cut(s) 2534
Eco88I CYCGRG 2 cut(s) 1190, 1271
EcoO109I RGGNCCY 1 cut(s) 1848
EcoRI GAATTC 1 cut(s) 2148
EcoRII CCWGG 6 cut(s) 106, 538, 664, 2137, 2412, 2527
EcoRV GATATC 1 cut(s) 700
EcoT14I CCWWGG 1 cut(s) 747
EcoT22I ATGCAT 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 747
FaeI CATG 5 cut(s) 161, 611, 751, 1578, 2267
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 4 cut(s) 107, 168, 637, 2434
FatI CATG 5 cut(s) 157, 607, 747, 1574, 2263
FauI CCCGC 1 cut(s) 893
FauNDI CATATG 1 cut(s) 2632
FbaI TGATCA 1 cut(s) 2164
FblI GTMKAC 2 cut(s) 2196, 2370
Fnu4HI GCNGC 1 cut(s) 2053
FokI GGATG 6 cut(s) 422, 1119, 1365, 1482, 1946, 2632
Fsp4HI GCNGC 1 cut(s) 2053
FspBI CTAG 6 cut(s) 437, 518, 533, 1590, 2106, 2582
GluI GCNGC 1 cut(s) 2053
HaeIII GGCC 5 cut(s) 858, 1365, 2318, 2527, 2532
HapII CCGG 2 cut(s) 1693, 1908
Hin1II CATG 5 cut(s) 161, 611, 751, 1578, 2267
HincII GTYRAC 1 cut(s) 1828
HindII GTYRAC 1 cut(s) 1828
HindIII AAGCTT 4 cut(s) 241, 1304, 1454, 1859
HpaI GTTAAC 1 cut(s) 1828
HpaII CCGG 2 cut(s) 1693, 1908
HphI GGTGA 5 cut(s) 212, 1766, 2420, 2551, 2675
Hpy166II GTNNAC 4 cut(s) 671, 1828, 2197, 2371
Hpy188I TCNGA 9 cut(s) 420, 743, 1142, 1449, 1645, 1809, 1866, 2425, 2562
Hpy8I GTNNAC 4 cut(s) 671, 1828, 2197, 2371
Hpy99I CGWCG 1 cut(s) 648
HpyAV CCTTC 6 cut(s) 484, 986, 1097, 1596, 1841, 2536
HpyCH4III ACNGT 4 cut(s) 600, 2194, 2273, 2489
HpyCH4IV ACGT 3 cut(s) 840, 1546, 2557
HpyF10VI GCNNNNNNNGC 5 cut(s) 613, 1362, 1413, 1775, 2578
HpySE526I ACGT 3 cut(s) 840, 1546, 2557
Hsp92II CATG 5 cut(s) 161, 611, 751, 1578, 2267
Kpn2I TCCGGA 1 cut(s) 1907
KpnI GGTACC 1 cut(s) 907
Ksp22I TGATCA 1 cut(s) 2164
KspAI GTTAAC 1 cut(s) 1828
Kzo9I GATC 9 cut(s) 499, 844, 914, 1096, 1459, 1645, 1726, 2164, 2425
LmnI GCTCC 5 cut(s) 891, 897, 995, 1256, 1615
Lsp1109I GCAGC 1 cut(s) 2039
LweI GCATC 4 cut(s) 157, 594, 1343, 1851
MaeI CTAG 6 cut(s) 437, 518, 533, 1590, 2106, 2582
MaeII ACGT 3 cut(s) 840, 1546, 2557
MaeIII GTNAC 7 cut(s) 124, 505, 836, 1739, 1754, 2057, 2110
MalI GATC 9 cut(s) 501, 846, 916, 1098, 1461, 1647, 1728, 2166, 2427
MbiI CCGCTC 1 cut(s) 900
MboI GATC 9 cut(s) 499, 844, 914, 1096, 1459, 1645, 1726, 2164, 2425
MfeI CAATTG 2 cut(s) 1347, 2023
MflI RGATCY 4 cut(s) 844, 914, 1096, 1726
MhlI GDGCHC 1 cut(s) 2271
MlyI GAGTC 4 cut(s) 659, 1195, 1451, 2429
MmeI TCCRAC 2 cut(s) 1120, 2647
Mph1103I ATGCAT 1 cut(s) 609
MroI TCCGGA 1 cut(s) 1907
MroXI GAANNNNTTC 5 cut(s) 207, 242, 639, 2121, 2679
MslI CAYNNNNRTG 1 cut(s) 752
MspCI CTTAAG 2 cut(s) 910, 1735
MspI CCGG 2 cut(s) 1693, 1908
MspR9I CCNGG 6 cut(s) 108, 540, 666, 2139, 2414, 2529
MunI CAATTG 2 cut(s) 1347, 2023
MvaI CCWGG 6 cut(s) 108, 540, 666, 2139, 2414, 2529
MwoI GCNNNNNNNGC 5 cut(s) 613, 1362, 1413, 1775, 2578
NcoI CCATGG 1 cut(s) 747
NdeI CATATG 1 cut(s) 2632
NdeII GATC 9 cut(s) 499, 844, 914, 1096, 1459, 1645, 1726, 2164, 2425
NlaIII CATG 5 cut(s) 161, 611, 751, 1578, 2267
NlaIV GGNNCC 3 cut(s) 887, 905, 1617
NmuCI GTSAC 3 cut(s) 505, 1754, 2110
NsiI ATGCAT 1 cut(s) 609
PaeR7I CTCGAG 1 cut(s) 1190
PceI AGGCCT 2 cut(s) 858, 2532
PdmI GAANNNNTTC 5 cut(s) 207, 242, 639, 2121, 2679
PfeI GAWTC 7 cut(s) 55, 1168, 1195, 1220, 1267, 1378, 2659
PflMI CCANNNNNTGG 1 cut(s) 357
PfoI TCCNGGA 1 cut(s) 2137
PkrI GCNGC 1 cut(s) 2054
PleI GAGTC 4 cut(s) 658, 1194, 1450, 2428
PpsI GAGTC 4 cut(s) 658, 1194, 1450, 2428
PpuMI RGGWCCY 1 cut(s) 1848
PshBI ATTAAT 1 cut(s) 1872
PsiI TTATAA 1 cut(s) 1541
Psp5II RGGWCCY 1 cut(s) 1848
Psp6I CCWGG 6 cut(s) 106, 538, 664, 2137, 2412, 2527
PspGI CCWGG 6 cut(s) 106, 538, 664, 2137, 2412, 2527
PspN4I GGNNCC 3 cut(s) 887, 905, 1617
PspPI GGNCC 4 cut(s) 104, 1578, 1848, 2525
PspPPI RGGWCCY 1 cut(s) 1848
PstNI CAGNNNCTG 1 cut(s) 1851
PsuI RGATCY 4 cut(s) 844, 914, 1096, 1726
RsaI GTAC 6 cut(s) 482, 905, 2259, 2470, 2556, 2719
RsaNI GTAC 6 cut(s) 481, 904, 2258, 2469, 2555, 2718
RseI CAYNNNNRTG 1 cut(s) 752
SatI GCNGC 1 cut(s) 2053
Sau3AI GATC 9 cut(s) 499, 844, 914, 1096, 1459, 1645, 1726, 2164, 2425
Sau96I GGNCC 4 cut(s) 104, 1578, 1848, 2525
SchI GAGTC 4 cut(s) 659, 1195, 1451, 2429
ScrFI CCNGG 6 cut(s) 108, 540, 666, 2139, 2414, 2529
SduI GDGCHC 1 cut(s) 2271
SfaNI GCATC 4 cut(s) 157, 594, 1343, 1851
SfcI CTRYAG 3 cut(s) 1659, 2193, 2750
Sfr274I CTCGAG 1 cut(s) 1190
SinI GGWCC 3 cut(s) 104, 1578, 1848
SlaI CTCGAG 1 cut(s) 1190
SmiMI CAYNNNNRTG 1 cut(s) 752
SmlI CTYRAG 6 cut(s) 560, 910, 1085, 1190, 1735, 2501
SmoI CTYRAG 6 cut(s) 560, 910, 1085, 1190, 1735, 2501
SseBI AGGCCT 2 cut(s) 858, 2532
SsiI CCGC 2 cut(s) 13, 900
SspI AATATT 1 cut(s) 1568
SspMI CTAG 6 cut(s) 437, 518, 533, 1590, 2106, 2582
StuI AGGCCT 2 cut(s) 858, 2532
StyD4I CCNGG 6 cut(s) 106, 538, 664, 2137, 2412, 2527
StyI CCWWGG 1 cut(s) 747
TaaI ACNGT 4 cut(s) 600, 2194, 2273, 2489
TaiI ACGT 3 cut(s) 843, 1549, 2560
TaqI TCGA 5 cut(s) 602, 646, 1191, 1483, 2170
TaqII GACCGA 1 cut(s) 92
TatI WGTACW 2 cut(s) 480, 2257
TfiI GAWTC 7 cut(s) 55, 1168, 1195, 1220, 1267, 1378, 2659
TscAI CASTG 3 cut(s) 1380, 1765, 1965
TseFI GTSAC 3 cut(s) 505, 1754, 2110
TseI GCWGC 1 cut(s) 2052
Tsp45I GTSAC 3 cut(s) 505, 1754, 2110
TspDTI ATGAA 9 cut(s) 131, 369, 867, 1012, 1118, 1304, 1643, 2008, 2175
TspGWI ACGGA 1 cut(s) 2129
TspRI CASTG 3 cut(s) 1380, 1765, 1965
Van91I CCANNNNNTGG 1 cut(s) 357
Vha464I CTTAAG 2 cut(s) 910, 1735
VpaK11BI GGWCC 3 cut(s) 104, 1578, 1848
VspI ATTAAT 1 cut(s) 1872
XapI RAATTY 5 cut(s) 805, 928, 1003, 1717, 2148
XbaI TCTAGA 1 cut(s) 1589
XhoI CTCGAG 1 cut(s) 1190
XmiI GTMKAC 2 cut(s) 2196, 2370
XmnI GAANNNNTTC 5 cut(s) 207, 242, 639, 2121, 2679
XspI CTAG 6 cut(s) 437, 518, 533, 1590, 2106, 2582
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.