RLG00000030358

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000105
Physical Location & Seq
Reverse (-)
60047058 .. 60050150
3093 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030358

Sequence Viewer

Length: 2133 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTCTGAGTTTCAGGGGGGAAGATACCCGTAGTGGTTTTACCGATCACCTGTATGCTGCACTCATTCAGAGAGGAATTCTCACCTTTCGGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAAGCCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATCTGCGATTGTCATTCTCTCACGGGGCTATGCTCGTTCCTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGATATGGGTCAACAAGTCTTCCCCGTCTTCTGCTTGGTGAGTCCTTCTGATGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAATGGGAACCTCAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATGAGGACAGACTAAATGCGTGGAGAGCTGCTTTGACAGAGGTGGCCAATCTTTCTGGCTGGGAGTATGCAGATGGTTCAAAACTAATTGAGGAAATTGGTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTAATTATTTATTAAGCAAGTATGTATATCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGGATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCTTCATGAAATTTGTCAAGATTTTGACCGAACCTGCTTTCTTTCTAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCTAAAATTGAAAATTTAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGCGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATCATCATAACCACCACAGATATTCAATTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTCACCTTTTGAGTTTGAAGGCTTTCAAGAAATGTTCCCCACCAGAAGATTATTTGCATCTGTGCTACCGTATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGAACGGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACGCCACATAAACGAATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCATCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCTGATGACAAAGAACTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAAAAATAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAAAATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTTGATCTTGAGGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTTTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGACGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTCAATGCTCTATCTTGATGGGACTGCTATTAAGGAACTGCCTATTTCAATTGAACGGCTGAGTGGCCTTGTGTCACTGAATCTAAGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATGAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAGACTGATGTGAATAGTGGGTGTCTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

711

Amino Acids

80.59

Weight (kDa)

6.82

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 186 6.6e-46 TIR domain
TIR_2 PF13676 14 - 107 8e-13 TIR domain
NB-ARC PF00931 212 - 366 1.1e-17 NB-ARC domain
AAA_22 PF13401 214 - 313 2.1e-06 AAA domain
NACHT PF05729 216 - 312 1e-06 NACHT domain
WHD_ROQ1 PF23282 433 - 505 2e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 602 - 686 3.8e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 613 - 682 1.8e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1646
Acc36I ACCTGC 2 cut(s) 740, 1646
AccB1I GGYRCC 1 cut(s) 342
AccI GTMKAC 1 cut(s) 545
AciI CCGC 6 cut(s) 340, 589, 623, 664, 871, 911
AclI AACGTT 1 cut(s) 1879
AclWI GGATC 4 cut(s) 646, 659, 921, 1365
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 4 cut(s) 117, 708, 829, 1588
AcuI CTGAAG 1 cut(s) 1595
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 629, 1403
AjnI CCWGG 2 cut(s) 964, 1525
AluBI AGCT 8 cut(s) 145, 182, 364, 446, 628, 695, 854, 1741
AluI AGCT 8 cut(s) 145, 182, 364, 446, 628, 695, 854, 1741
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 6 cut(s) 1518, 1554, 1634, 1667, 1778, 2096
AlwI GGATC 4 cut(s) 646, 659, 921, 1365
AlwNI CAGNNNCTG 1 cut(s) 854
Ama87I CYCGRG 3 cut(s) 690, 1775, 1856
AoxI GGCC 3 cut(s) 462, 772, 1948
ApeKI GCWGC 5 cut(s) 98, 182, 446, 625, 854
ApoI RAATTY 4 cut(s) 117, 708, 829, 1588
AseI ATTAAT 2 cut(s) 594, 1820
Asp700I GAANNNNTTC 5 cut(s) 583, 795, 1065, 1088, 1500
AspS9I GGNCC 1 cut(s) 962
AsuHPI GGTGA 4 cut(s) 80, 115, 334, 1061
AvaI CYCGRG 3 cut(s) 690, 1775, 1856
AvaII GGWCC 1 cut(s) 962
BalI TGGCCA 1 cut(s) 464
BamHI GGATCC 1 cut(s) 651
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1172
BbsI GAAGAC 4 cut(s) 295, 304, 1563, 1985
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 5 cut(s) 85, 169, 433, 637, 841
BccI CCATC 7 cut(s) 485, 808, 1283, 1395, 1722, 1895, 2080
BceAI ACGGC 1 cut(s) 1955
BcgI CGANNNNNNTGC 2 cut(s) 1756, 1790
BciT130I CCWGG 2 cut(s) 966, 1527
BcoDI GTCTC 6 cut(s) 1518, 1554, 1634, 1667, 1778, 2096
BfaI CTAG 3 cut(s) 198, 776, 1376
BfmI CTRYAG 1 cut(s) 1975
BfuAI ACCTGC 2 cut(s) 740, 1646
BglII AGATCT 2 cut(s) 200, 1427
BisI GCNGC 6 cut(s) 99, 183, 341, 447, 626, 855
BlsI GCNGC 6 cut(s) 100, 184, 342, 448, 627, 856
Bme1390I CCNGG 2 cut(s) 966, 1527
Bme18I GGWCC 1 cut(s) 962
BmeT110I CYCGRG 3 cut(s) 690, 1775, 1856
BmgT120I GGNCC 1 cut(s) 962
BmiI GGNNCC 3 cut(s) 344, 375, 653
BmrFI CCNGG 2 cut(s) 966, 1527
BmsI GCATC 6 cut(s) 651, 898, 1015, 1132, 1455, 2036
BpiI GAAGAC 4 cut(s) 295, 304, 1563, 1985
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu10I CCTNAGC 1 cut(s) 1152
BpuEI CTTGAG 4 cut(s) 167, 363, 1691, 1706
BsaBI GATNNNNATC 2 cut(s) 912, 1356
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1554, 1634
BsaJI CCNNGG 3 cut(s) 655, 965, 1723
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 1403
Bse1I ACTGG 2 cut(s) 959, 1299
Bse8I GATNNNNATC 2 cut(s) 912, 1356
BseBI CCWGG 2 cut(s) 966, 1527
BseDI CCNNGG 3 cut(s) 655, 965, 1723
BseGI GGATG 9 cut(s) 139, 259, 666, 844, 1216, 1303, 1470, 1697, 2051
BseJI GATNNNNATC 2 cut(s) 912, 1356
BseLI CCNNNNNNNGG 2 cut(s) 629, 1403
BseMII CTCAG 3 cut(s) 38, 1166, 1934
BseNI ACTGG 2 cut(s) 959, 1299
BseRI GAGGAG 2 cut(s) 206, 2114
BseXI GCAGC 5 cut(s) 85, 169, 433, 637, 841
BseYI CCCAGC 2 cut(s) 477, 1491
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 3 cut(s) 464, 774, 1950
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 3 cut(s) 690, 1775, 1856
BslFI GGGAC 1 cut(s) 1918
BslI CCNNNNNNNGG 2 cut(s) 629, 1403
BsmAI GTCTC 6 cut(s) 1518, 1554, 1634, 1667, 1778, 2096
BsmFI GGGAC 1 cut(s) 1918
BsnI GGCC 3 cut(s) 464, 774, 1950
Bso31I GGTCTC 2 cut(s) 1554, 1634
BsoBI CYCGRG 3 cut(s) 690, 1775, 1856
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 7 cut(s) 85, 200, 651, 913, 1357, 1427, 1681
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 6 cut(s) 340, 589, 623, 664, 871, 911
BspANI GGCC 3 cut(s) 464, 774, 1950
BspCNI CTCAG 3 cut(s) 39, 1165, 1935
BspHI TCATGA 2 cut(s) 703, 1552
BspLI GGNNCC 3 cut(s) 344, 375, 653
BspMAI CTGCAG 1 cut(s) 1979
BspMI ACCTGC 2 cut(s) 740, 1646
BspPI GGATC 4 cut(s) 646, 659, 921, 1365
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 2 cut(s) 1554, 1634
BsrI ACTGG 2 cut(s) 959, 1299
BssECI CCNNGG 3 cut(s) 655, 965, 1723
BssMI GATC 7 cut(s) 85, 200, 651, 913, 1357, 1427, 1681
BssNAI GTATAC 1 cut(s) 546
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1172
BssT1I CCWWGG 2 cut(s) 655, 1723
Bst1107I GTATAC 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 1172
Bst2UI CCWGG 2 cut(s) 966, 1527
Bst4CI ACNGT 5 cut(s) 1055, 1136, 1461, 1512, 2093
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1474
BstC8I GCNNGC 1 cut(s) 1333
BstDEI CTNAG 4 cut(s) 47, 1152, 1943, 1967
BstDSI CCRYGG 1 cut(s) 655
BstF5I GGATG 9 cut(s) 139, 259, 666, 844, 1216, 1303, 1470, 1697, 2051
BstKTI GATC 7 cut(s) 88, 203, 654, 916, 1360, 1430, 1684
BstMAI GTCTC 6 cut(s) 1518, 1554, 1634, 1667, 1778, 2096
BstMBI GATC 7 cut(s) 85, 200, 651, 913, 1357, 1427, 1681
BstMWI GCNNNNNNNGC 2 cut(s) 443, 1474
BstNI CCWGG 2 cut(s) 966, 1527
BstSCI CCNGG 2 cut(s) 964, 1525
BstSFI CTRYAG 1 cut(s) 1975
BstV1I GCAGC 5 cut(s) 85, 169, 433, 637, 841
BstV2I GAAGAC 4 cut(s) 295, 304, 1563, 1985
BstX2I RGATCY 3 cut(s) 200, 651, 1427
BstYI RGATCY 3 cut(s) 200, 651, 1427
BstZ17I GTATAC 1 cut(s) 546
BsuRI GGCC 3 cut(s) 464, 774, 1950
BtgI CCRYGG 1 cut(s) 655
BtsCI GGATG 9 cut(s) 139, 259, 666, 844, 1216, 1303, 1470, 1697, 2051
BtsIMutI CAGTG 1 cut(s) 1958
BveI ACCTGC 2 cut(s) 740, 1646
Cac8I GCNNGC 1 cut(s) 1333
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 2 cut(s) 703, 1552
Cfr13I GGNCC 1 cut(s) 962
CseI GACGC 1 cut(s) 146
CviAII CATG 9 cut(s) 243, 280, 398, 656, 704, 1016, 1469, 1553, 2005
DdeI CTNAG 4 cut(s) 47, 1152, 1943, 1967
DpnI GATC 7 cut(s) 87, 202, 653, 915, 1359, 1429, 1683
DpnII GATC 7 cut(s) 85, 200, 651, 913, 1357, 1427, 1681
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 655, 1723
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 2 cut(s) 1554, 1634
Eco47I GGWCC 1 cut(s) 962
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 1595
Eco88I CYCGRG 3 cut(s) 690, 1775, 1856
EcoICRI GAGCTC 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 2 cut(s) 964, 1525
EcoT14I CCWWGG 2 cut(s) 655, 1723
EcoT22I ATGCAT 2 cut(s) 281, 1470
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 2 cut(s) 655, 1723
FaeI CATG 9 cut(s) 246, 283, 401, 659, 707, 1019, 1472, 1556, 2008
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 1 cut(s) 1918
FatI CATG 9 cut(s) 242, 279, 397, 655, 703, 1015, 1468, 1552, 2004
FauI CCCGC 2 cut(s) 596, 657
FblI GTMKAC 1 cut(s) 545
Fnu4HI GCNGC 6 cut(s) 99, 183, 341, 447, 626, 855
FokI GGATG 9 cut(s) 146, 266, 673, 851, 1223, 1310, 1477, 1704, 2058
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 6 cut(s) 99, 183, 341, 447, 626, 855
FspBI CTAG 3 cut(s) 198, 776, 1376
GluI GCNGC 6 cut(s) 99, 183, 341, 447, 626, 855
GsaI CCCAGC 2 cut(s) 481, 1495
HaeIII GGCC 3 cut(s) 464, 774, 1950
HgaI GACGC 1 cut(s) 146
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 9 cut(s) 246, 283, 401, 659, 707, 1019, 1472, 1556, 2008
HincII GTYRAC 4 cut(s) 9, 296, 562, 921
HindII GTYRAC 4 cut(s) 9, 296, 562, 921
HindIII AAGCTT 1 cut(s) 1739
HphI GGTGA 4 cut(s) 80, 115, 334, 1061
Hpy166II GTNNAC 6 cut(s) 9, 296, 546, 562, 921, 1508
Hpy188I TCNGA 6 cut(s) 48, 111, 334, 1278, 1447, 1665
Hpy8I GTNNAC 6 cut(s) 9, 296, 546, 562, 921, 1508
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 4 cut(s) 339, 710, 1078, 1342
HpyCH4III ACNGT 5 cut(s) 1055, 1136, 1461, 1512, 2093
HpyCH4IV ACGT 2 cut(s) 1534, 1879
HpyF10VI GCNNNNNNNGC 2 cut(s) 443, 1474
HpyF3I CTNAG 4 cut(s) 47, 1152, 1943, 1967
HpySE526I ACGT 2 cut(s) 1534, 1879
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 9 cut(s) 246, 283, 401, 659, 707, 1019, 1472, 1556, 2008
Kzo9I GATC 7 cut(s) 85, 200, 651, 913, 1357, 1427, 1681
LmnI GCTCC 1 cut(s) 851
Lsp1109I GCAGC 5 cut(s) 85, 169, 433, 637, 841
LweI GCATC 6 cut(s) 651, 898, 1015, 1132, 1455, 2036
MaeI CTAG 3 cut(s) 198, 776, 1376
MaeII ACGT 2 cut(s) 1534, 1879
MaeIII GTNAC 2 cut(s) 1363, 1956
MalI GATC 7 cut(s) 87, 202, 653, 915, 1359, 1429, 1683
MboI GATC 7 cut(s) 85, 200, 651, 913, 1357, 1427, 1681
MfeI CAATTG 3 cut(s) 186, 1001, 1932
MflI RGATCY 3 cut(s) 200, 651, 1427
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 1 cut(s) 464
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 2 cut(s) 203, 334
MmeI TCCRAC 1 cut(s) 1846
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 2 cut(s) 281, 1470
MroXI GAANNNNTTC 5 cut(s) 583, 795, 1065, 1088, 1500
MscI TGGCCA 1 cut(s) 464
MslI CAYNNNNRTG 2 cut(s) 93, 578
Msp20I TGGCCA 1 cut(s) 464
MspR9I CCNGG 2 cut(s) 966, 1527
MunI CAATTG 3 cut(s) 186, 1001, 1932
MvaI CCWGG 2 cut(s) 966, 1527
MwoI GCNNNNNNNGC 2 cut(s) 443, 1474
NcoI CCATGG 1 cut(s) 655
NdeII GATC 7 cut(s) 85, 200, 651, 913, 1357, 1427, 1681
NlaIII CATG 9 cut(s) 246, 283, 401, 659, 707, 1019, 1472, 1556, 2008
NlaIV GGNNCC 3 cut(s) 344, 375, 653
NmeAIII GCCGAG 1 cut(s) 2037
NmuCI GTSAC 2 cut(s) 1363, 1956
NsiI ATGCAT 2 cut(s) 281, 1470
PaeR7I CTCGAG 2 cut(s) 690, 1775
PagI TCATGA 2 cut(s) 703, 1552
PaqCI CACCTGC 1 cut(s) 1646
PdmI GAANNNNTTC 5 cut(s) 583, 795, 1065, 1088, 1500
PfeI GAWTC 9 cut(s) 584, 807, 975, 1666, 1710, 1753, 1805, 1852, 1963
PkrI GCNGC 6 cut(s) 100, 184, 342, 448, 627, 856
PleI GAGTC 2 cut(s) 202, 333
PpsI GAGTC 2 cut(s) 202, 333
PshBI ATTAAT 2 cut(s) 594, 1820
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 1879
Psp6I CCWGG 2 cut(s) 964, 1525
PspFI CCCAGC 2 cut(s) 477, 1491
PspGI CCWGG 2 cut(s) 964, 1525
PspN4I GGNNCC 3 cut(s) 344, 375, 653
PspPI GGNCC 1 cut(s) 962
PspXI VCTCGAGB 1 cut(s) 690
PstI CTGCAG 1 cut(s) 1979
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 3 cut(s) 200, 651, 1427
RseI CAYNNNNRTG 2 cut(s) 93, 578
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 6 cut(s) 99, 183, 341, 447, 626, 855
Sau3AI GATC 7 cut(s) 85, 200, 651, 913, 1357, 1427, 1681
Sau96I GGNCC 1 cut(s) 962
SchI GAGTC 2 cut(s) 203, 334
ScrFI CCNGG 2 cut(s) 966, 1527
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 6 cut(s) 651, 898, 1015, 1132, 1455, 2036
SfcI CTRYAG 1 cut(s) 1975
Sfr274I CTCGAG 2 cut(s) 690, 1775
SinI GGWCC 1 cut(s) 962
SlaI CTCGAG 2 cut(s) 690, 1775
SmiMI CAYNNNNRTG 2 cut(s) 93, 578
SmlI CTYRAG 6 cut(s) 146, 378, 690, 1670, 1685, 1775
SmoI CTYRAG 6 cut(s) 146, 378, 690, 1670, 1685, 1775
SsiI CCGC 6 cut(s) 340, 589, 623, 664, 871, 911
SspI AATATT 1 cut(s) 1317
SspMI CTAG 3 cut(s) 198, 776, 1376
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 2 cut(s) 964, 1525
StyI CCWWGG 2 cut(s) 655, 1723
TaaI ACNGT 5 cut(s) 1055, 1136, 1461, 1512, 2093
TaiI ACGT 2 cut(s) 1537, 1882
TaqI TCGA 2 cut(s) 691, 1776
TaqII GACCGA 1 cut(s) 741
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 9 cut(s) 584, 807, 975, 1666, 1710, 1753, 1805, 1852, 1963
TscAI CASTG 1 cut(s) 1965
TseFI GTSAC 2 cut(s) 1363, 1956
TseI GCWGC 5 cut(s) 98, 182, 446, 625, 854
Tsp45I GTSAC 2 cut(s) 1363, 1956
TspGWI ACGGA 3 cut(s) 529, 1152, 1223
TspRI CASTG 1 cut(s) 1965
VpaK11BI GGWCC 1 cut(s) 962
VspI ATTAAT 2 cut(s) 594, 1820
XapI RAATTY 4 cut(s) 117, 708, 829, 1588
XbaI TCTAGA 1 cut(s) 197
XcmI CCANNNNNNNNNTGG 2 cut(s) 811, 1721
XhoI CTCGAG 2 cut(s) 690, 1775
XmiI GTMKAC 1 cut(s) 545
XmnI GAANNNNTTC 5 cut(s) 583, 795, 1065, 1088, 1500
XspI CTAG 3 cut(s) 198, 776, 1376
Zsp2I ATGCAT 2 cut(s) 281, 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.