RLG00000015531

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000105
Physical Location & Seq
Reverse (-)
67580009 .. 67586777
6769 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015531

Sequence Viewer

Length: 2637 bp
ATGACGTCAACTTGGTCTCCCCGGCGACGAAAGAAATACGATGTTTTTCTAAGCTTCAGAGGGGAAGATACCCGCGAGACTTTCATCTGCCATCTGTATCGTGCACTAACTGGGAGAGGAATTATAACCTTCTTAGATGACGAAAGCCTGGAGAGAGGATCAACCATTGGCCCATCACTTTTTACAGCAATTCAGGACTCAAGAATGTCCCTTGTCGTTCTTTCACAGAATTTCGCTTCTTCATCCTGGTGCTTGGATGAACTCTCCAAGATTCTTCAATGGACGGAAGAGACGGGACAAAGAGTCGTCCCAATCTTCTACGGAGTGGATCCTTCTGATGTGCGCAAGCAAAGGAGAAGTTTTCAGCTCCGTACAGTAGAGGTAAGGGAACATGAAGAAGTTTATGGGAATGAGGACAAGCTAAAGGAGTGGATAGTTTCTTTGAGACGAGTGGCCGATATTTCTGGGTATGATTCGAGGAAATATAAGGATGACGGAAAATTGATCGAGGAAATTGTTACGGATACACTAAGAAGTCTACGTGATACTTATACCTCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTTATGATTTATTAAGCAATTACATATGTCCGGAGCTTGGCGGGGTGCGCTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATTTATGATGGACTTTCTCACGGGTTTGAAAGAAGCTGCTTTCTTTTAAATGTTAGAGAAACATCTGAAAAGGATGGCCTAGTTTCTTTACAAGAAAAACTACTTTCCAGAATCCTGAGGACAAAAGTTGACATTGAGGATGAATATGCAGGAGCTACTATGATAGAAAAGCAGTTATGTGAACAAAAGGTCCTTGTTGTTATTGATGATGTGGATCACTTCAATCAATTAGATAAATTGGCTAGAAGTCGGGACTGGTTTGGTCCAGGGAGTAGAATCATTGTAACCACCCCAGATATTCATTTGCTAAGGGGACATGATGTTACATACGAGGCTACTGGGTTAACTGATGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCCAGAAAAGCTTCCCACCAGAAGATTATTTGGACTTGTGCCACCATATTATAGGGTATGCTCAGGGGCTTCCGTTGGCTCTTGTTGTTTTAGGTTCTTTTCTACGTGGTAGAAGCACCAATGAATGGGAAAGTACAATAGAGAGGCTACACAATACCCCAAATGGACAAATTATGGATGTGCTTCGGATTAGTTTTGATGGACTAGAGGAAAAAGATCAACAGATATTCCTACATATTGCTTGTTTGTACAAGGGGAAGGATAGGGATCGTGTGACACAAATACTAGACTATTGCAAGCTAAACCCTGGCATTGGTTTAAGTGTTCTTGAGGAAAAATCTCTAATTACTATTTTTAAGAACAAACTGTCGATGCATGATTTGCTACAAGACATGGGCTTGGAAATAGTTCGTCGAGAGTCCCCTGATGAGCCAGGTGAACGAAGTAGATTATGGTCTCCTGAAGTTATCCACAATGTGCTGAAGAAAAATAAGACCCCAGACCTCAGCGGCGTTCAAAATCTTGAGACTTTAGATCTTGAAGGATGTAAGACTTTGGTAACACTTCATGAATCCCTCGGACAACTCAAAAGGCTTATTATATTGAATCTTAAAGATTGCGAGAGTCTCAAGAGTCTGCCAAGTAAGATTGAAATGGAATCTCTTCAAACTTTAATTCTTTCTAACTGCTCGAAAGTTATAAGGATTCCTGAGTTCGCTGGAAATATGGAGCGTTTATCAGAGCTTTATCTCAATGGGACAGCCATTGAAAAACTACCTGTTTCAATTGGATGCCTGAGTGGCCTTACTTTGCTGAATCTGAGCAACTGCAAAAATCTTGATTGCCTTCCAAGCACCTTTAGTAGATTGGAGTCTCTTGAAAAGCTTGATCTTTCTGGATGTTTGAAACTTGGAGAACAGGTTAGTGTGAGGGAGAATGGTGCAATTAGAACTGCTGTAAATCATCTTACAAAACGCTTTTGTGAAGTTGTTGGGAAATATTTGTCATGGTTCTTGCCTTCTAGATTGGTGCAAAGAGCCGATATAGAACCAGTGAGTATGCAGTTTCCTGTATCTGATCTGTGTTATTCTACAGAGCAAAATGCAAATACATCCATGAGTATCCGGTTGCCATTACCTCGTCTATATCTAAATATAAGTAACTGCAATCTTGATGACGCTGCATTTGCCAACTTTGCCTGTTTTCCCTCCTTAGTGGCGCTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTGAAGCTTCAGAACATTAATTTGGAGAATTGCAAGACACTTCAAGAGTTGTCAGGACTTCCATCGAATGGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAAATGCTGTTTGATGTATCCAATTTCAATATATTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTGCGGCCTCTCATCTGCATTTGCACCTCTGGAGGAGGAGGAGGATGCAGAACTTCCCTGGCTGGTCCTCATAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

879

Amino Acids

99.12

Weight (kDa)

5.52

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 193 2.8e-48 TIR domain
TIR_2 PF13676 15 - 124 3.3e-14 TIR domain
NB-ARC PF00931 214 - 366 5.3e-17 NB-ARC domain
AAA_22 PF13401 216 - 321 5.6e-06 AAA domain
NACHT PF05729 218 - 314 5.4e-09 NACHT domain
WHD_ROQ1 PF23282 432 - 503 1.5e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 582 - 669 1.9e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 736 - 829 6.2e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 125, 1787
AatII GACGTC 1 cut(s) 8
Acc16I TGCGCA 1 cut(s) 344
AccB7I CCANNNNNTGG 2 cut(s) 1212, 2429
AccI GTMKAC 1 cut(s) 538
AccII CGCG 1 cut(s) 75
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 6 cut(s) 73, 595, 639, 670, 1596, 2562
AclWI GGATC 7 cut(s) 166, 323, 336, 652, 665, 924, 1362
AcoI YGGCCR 1 cut(s) 453
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 4 cut(s) 40, 1569, 1589, 2354
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1564
AfaI GTAC 3 cut(s) 373, 1222, 1337
AfiI CCNNNNNNNGG 4 cut(s) 635, 1212, 1400, 2429
AhdI GACNNNNNGTC 1 cut(s) 302
AjnI CCWGG 6 cut(s) 147, 245, 967, 1393, 1519, 2615
AjuI GAANNNNNNNTTGG 4 cut(s) 2366, 2398, 2420, 2452
AloI GAACNNNNNNTCC 2 cut(s) 876, 908
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 8 cut(s) 21, 71, 284, 439, 1548, 1607, 1718, 1965
Alw44I GTGCAC 1 cut(s) 102
AlwI GGATC 7 cut(s) 166, 323, 336, 652, 665, 924, 1362
AlwNI CAGNNNCTG 1 cut(s) 2358
Ama87I CYCGRG 1 cut(s) 696
Aor13HI TCCGGA 1 cut(s) 628
AoxI GGCC 5 cut(s) 169, 453, 778, 1888, 2563
ApaLI GTGCAC 1 cut(s) 102
ApeKI GCWGC 3 cut(s) 738, 2267, 2559
ApoI RAATTY 1 cut(s) 229
ArsI GACNNNNNNTTYG 2 cut(s) 2255, 2287
AseI ATTAAT 1 cut(s) 2379
Asp700I GAANNNNTTC 5 cut(s) 589, 1062, 1097, 1494, 1749
AspLEI GCGC 3 cut(s) 345, 648, 2308
AspS9I GGNCC 5 cut(s) 170, 892, 965, 2355, 2623
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 2 cut(s) 1067, 1535
AvaI CYCGRG 1 cut(s) 696
AvaII GGWCC 4 cut(s) 892, 965, 2355, 2623
AxyI CCTNAGG 1 cut(s) 818
BaeGI GKGCMC 1 cut(s) 106
BamHI GGATCC 2 cut(s) 328, 657
Bbv12I GWGCWC 1 cut(s) 106
BbvCI CCTCAGC 1 cut(s) 1592
BbvI GCAGC 3 cut(s) 725, 2254, 2546
BccI CCATC 8 cut(s) 99, 181, 704, 770, 1046, 1280, 2431, 2447
BciT130I CCWGG 6 cut(s) 149, 247, 969, 1395, 1521, 2617
BciVI GTATCC 3 cut(s) 517, 2219, 2497
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 8 cut(s) 21, 71, 284, 439, 1548, 1607, 1718, 1965
BfaI CTAG 5 cut(s) 782, 945, 1292, 1373, 2109
BfmI CTRYAG 1 cut(s) 2178
BfoI RGCGCY 1 cut(s) 2309
BfuI GTATCC 3 cut(s) 517, 2219, 2497
BglI GCCNNNNNGGC 1 cut(s) 1887
BglII AGATCT 1 cut(s) 1621
BisI GCNGC 5 cut(s) 739, 1597, 2268, 2560, 2563
BlsI GCNGC 5 cut(s) 740, 1598, 2269, 2561, 2564
Bme1390I CCNGG 7 cut(s) 22, 149, 247, 969, 1395, 1521, 2617
Bme18I GGWCC 4 cut(s) 892, 965, 2355, 2623
BmeRI GACNNNNNGTC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 696
BmgT120I GGNCC 5 cut(s) 170, 892, 965, 2355, 2623
BmiI GGNNCC 2 cut(s) 330, 659
BmrFI CCNGG 7 cut(s) 22, 149, 247, 969, 1395, 1521, 2617
BmrI ACTGGG 2 cut(s) 120, 1050
BmsI GCATC 4 cut(s) 1449, 1868, 2358, 2593
BmuI ACTGGG 2 cut(s) 120, 1050
BpmI CTGGAG 2 cut(s) 170, 2609
Bpu10I CCTNAGC 3 cut(s) 1010, 1149, 1592
BpuEI CTTGAG 4 cut(s) 184, 1436, 1631, 1700
BpuMI CCSGG 1 cut(s) 22
BsaAI YACGTR 2 cut(s) 542, 1193
BsaBI GATNNNNATC 2 cut(s) 915, 1353
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 21, 1548
BsaJI CCNNGG 6 cut(s) 20, 661, 968, 1393, 1663, 2615
BsaWI WCCGGW 2 cut(s) 628, 2211
BsaXI ACNNNNNCTCC 3 cut(s) 31, 1992, 2022
Bsc4I CCNNNNNNNGG 4 cut(s) 635, 1212, 1400, 2429
Bse1I ACTGG 5 cut(s) 115, 962, 1045, 2138, 2472
Bse21I CCTNAGG 1 cut(s) 818
Bse8I GATNNNNATC 2 cut(s) 915, 1353
BseAI TCCGGA 1 cut(s) 628
BseBI CCWGG 6 cut(s) 149, 247, 969, 1395, 1521, 2617
BseDI CCNNGG 6 cut(s) 20, 661, 968, 1393, 1663, 2615
BseJI GATNNNNATC 2 cut(s) 915, 1353
BseLI CCNNNNNNNGG 4 cut(s) 635, 1212, 1400, 2429
BseMII CTCAG 7 cut(s) 809, 1163, 1606, 1788, 1874, 1898, 2306
BseNI ACTGG 5 cut(s) 115, 962, 1045, 2138, 2472
BseRI GAGGAG 3 cut(s) 2606, 2609, 2612
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 3 cut(s) 725, 2254, 2546
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 5 cut(s) 171, 455, 780, 1890, 2565
BsiHKAI GWGCWC 1 cut(s) 106
BsiHKCI CYCGRG 1 cut(s) 696
BsiSI CCGG 3 cut(s) 22, 629, 2212
BslFI GGGAC 7 cut(s) 193, 293, 309, 968, 1029, 1492, 1858
BslI CCNNNNNNNGG 4 cut(s) 635, 1212, 1400, 2429
BsmAI GTCTC 8 cut(s) 21, 71, 284, 439, 1548, 1607, 1718, 1965
BsmBI CGTCTC 2 cut(s) 284, 439
BsmFI GGGAC 7 cut(s) 193, 293, 309, 968, 1029, 1492, 1858
BsnI GGCC 5 cut(s) 171, 455, 780, 1890, 2565
Bso31I GGTCTC 2 cut(s) 21, 1548
BsoBI CYCGRG 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 106
Bsp13I TCCGGA 1 cut(s) 628
Bsp1407I TGTACA 1 cut(s) 1335
Bsp19I CCATGG 1 cut(s) 661
BspACI CCGC 6 cut(s) 73, 595, 639, 670, 1596, 2562
BspANI GGCC 5 cut(s) 171, 455, 780, 1890, 2565
BspCNI CTCAG 7 cut(s) 810, 1162, 1605, 1789, 1875, 1899, 2307
BspEI TCCGGA 1 cut(s) 628
BspFNI CGCG 1 cut(s) 75
BspHI TCATGA 1 cut(s) 1654
BspLI GGNNCC 2 cut(s) 330, 659
BspPI GGATC 7 cut(s) 166, 323, 336, 652, 665, 924, 1362
BspTNI GGTCTC 2 cut(s) 21, 1548
BsrGI TGTACA 1 cut(s) 1335
BsrI ACTGG 5 cut(s) 115, 962, 1045, 2138, 2472
BssECI CCNNGG 6 cut(s) 20, 661, 968, 1393, 1663, 2615
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 661
Bst2UI CCWGG 6 cut(s) 149, 247, 969, 1395, 1521, 2617
Bst4CI ACNGT 2 cut(s) 376, 1455
Bst6I CTCTTC 3 cut(s) 282, 562, 1755
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1468
BstAUI TGTACA 1 cut(s) 1335
BstBAI YACGTR 2 cut(s) 542, 1193
BstC8I GCNNGC 3 cut(s) 347, 668, 1385
BstDSI CCRYGG 1 cut(s) 661
BstFNI CGCG 1 cut(s) 75
BstH2I RGCGCY 1 cut(s) 2309
BstHHI GCGC 3 cut(s) 345, 648, 2308
BstMAI GTCTC 8 cut(s) 21, 71, 284, 439, 1548, 1607, 1718, 1965
BstMWI GCNNNNNNNGC 7 cut(s) 645, 672, 1468, 1887, 1938, 2273, 2282
BstNI CCWGG 6 cut(s) 149, 247, 969, 1395, 1521, 2617
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 7 cut(s) 20, 147, 245, 967, 1393, 1519, 2615
BstSFI CTRYAG 1 cut(s) 2178
BstSLI GKGCMC 1 cut(s) 106
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 725, 2254, 2546
BstX2I RGATCY 3 cut(s) 328, 657, 1621
BstYI RGATCY 3 cut(s) 328, 657, 1621
Bsu36I CCTNAGG 1 cut(s) 818
BsuI GTATCC 3 cut(s) 517, 2219, 2497
BsuRI GGCC 5 cut(s) 171, 455, 780, 1890, 2565
BtgI CCRYGG 1 cut(s) 661
BtsIMutI CAGTG 2 cut(s) 2145, 2465
Cac8I GCNNGC 3 cut(s) 347, 668, 1385
CaiI CAGNNNCTG 1 cut(s) 2358
CciI TCATGA 1 cut(s) 1654
CfoI GCGC 3 cut(s) 345, 648, 2308
Cfr13I GGNCC 5 cut(s) 170, 892, 965, 2355, 2623
CseI GACGC 1 cut(s) 2273
Csp6I GTAC 3 cut(s) 372, 1221, 1336
CviQI GTAC 3 cut(s) 372, 1221, 1336
DraI TTTAAA 2 cut(s) 750, 1080
DraIII CACNNNGTG 1 cut(s) 1564
DriI GACNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 3 cut(s) 282, 562, 1755
Eam1105I GACNNNNNGTC 1 cut(s) 302
EarI CTCTTC 3 cut(s) 282, 562, 1755
Eco130I CCWWGG 1 cut(s) 661
Eco31I GGTCTC 2 cut(s) 21, 1548
Eco47I GGWCC 4 cut(s) 892, 965, 2355, 2623
Eco57I CTGAAG 4 cut(s) 40, 1569, 1589, 2354
Eco81I CCTNAGG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 696
EcoO109I RGGNCCY 2 cut(s) 892, 2355
EcoRII CCWGG 6 cut(s) 147, 245, 967, 1393, 1519, 2615
EcoT14I CCWWGG 1 cut(s) 661
EcoT22I ATGCAT 1 cut(s) 1464
ErhI CCWWGG 1 cut(s) 661
Esp3I CGTCTC 2 cut(s) 284, 439
FaqI GGGAC 7 cut(s) 193, 293, 309, 968, 1029, 1492, 1858
FauI CCCGC 4 cut(s) 80, 602, 632, 663
FauNDI CATATG 1 cut(s) 623
FblI GTMKAC 1 cut(s) 538
Fnu4HI GCNGC 5 cut(s) 739, 1597, 2268, 2560, 2563
Fsp4HI GCNGC 5 cut(s) 739, 1597, 2268, 2560, 2563
FspBI CTAG 5 cut(s) 782, 945, 1292, 1373, 2109
FspI TGCGCA 1 cut(s) 344
GlaI GCGC 3 cut(s) 344, 647, 2307
GluI GCNGC 5 cut(s) 739, 1597, 2268, 2560, 2563
GsuI CTGGAG 2 cut(s) 170, 2609
HaeII RGCGCY 1 cut(s) 2309
HaeIII GGCC 5 cut(s) 171, 455, 780, 1890, 2565
HapII CCGG 3 cut(s) 22, 629, 2212
HgaI GACGC 1 cut(s) 2273
HhaI GCGC 3 cut(s) 345, 648, 2308
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 3 cut(s) 343, 646, 2306
HinP1I GCGC 3 cut(s) 343, 646, 2306
HincII GTYRAC 4 cut(s) 9, 568, 832, 1047
HindII GTYRAC 4 cut(s) 9, 568, 832, 1047
HindIII AAGCTT 5 cut(s) 52, 1096, 1970, 2336, 2366
HpaI GTTAAC 1 cut(s) 1047
HpaII CCGG 3 cut(s) 22, 629, 2212
HphI GGTGA 2 cut(s) 1067, 1535
Hpy166II GTNNAC 8 cut(s) 9, 104, 539, 568, 832, 884, 1047, 1526
Hpy8I GTNNAC 8 cut(s) 9, 104, 539, 568, 832, 884, 1047, 1526
Hpy99I CGWCG 2 cut(s) 30, 1503
HpyAV CCTTC 6 cut(s) 139, 342, 1339, 1622, 1943, 2115
HpyCH4III ACNGT 2 cut(s) 376, 1455
HpyCH4IV ACGT 3 cut(s) 5, 541, 1192
HpyF10VI GCNNNNNNNGC 7 cut(s) 645, 672, 1468, 1887, 1938, 2273, 2282
HpySE526I ACGT 3 cut(s) 5, 541, 1192
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 3 cut(s) 343, 646, 2306
Kpn2I TCCGGA 1 cut(s) 628
KspAI GTTAAC 1 cut(s) 1047
LmnI GCTCC 4 cut(s) 372, 631, 854, 1816
Lsp1109I GCAGC 3 cut(s) 725, 2254, 2546
LweI GCATC 4 cut(s) 1449, 1868, 2358, 2593
MaeI CTAG 5 cut(s) 782, 945, 1292, 1373, 2109
MaeII ACGT 3 cut(s) 5, 541, 1192
MaeIII GTNAC 6 cut(s) 517, 985, 1024, 1360, 1645, 2246
MfeI CAATTG 2 cut(s) 1872, 2530
MflI RGATCY 3 cut(s) 328, 657, 1621
MhlI GDGCHC 1 cut(s) 106
MlyI GAGTC 6 cut(s) 191, 312, 1514, 1720, 1729, 1967
Mph1103I ATGCAT 1 cut(s) 1464
MroI TCCGGA 1 cut(s) 628
MroXI GAANNNNTTC 5 cut(s) 589, 1062, 1097, 1494, 1749
MslI CAYNNNNRTG 2 cut(s) 247, 584
MspA1I CMGCKG 1 cut(s) 1596
MspI CCGG 3 cut(s) 22, 629, 2212
MspR9I CCNGG 7 cut(s) 22, 149, 247, 969, 1395, 1521, 2617
MunI CAATTG 2 cut(s) 1872, 2530
MvaI CCWGG 6 cut(s) 149, 247, 969, 1395, 1521, 2617
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 7 cut(s) 645, 672, 1468, 1887, 1938, 2273, 2282
NciI CCSGG 1 cut(s) 22
NcoI CCATGG 1 cut(s) 661
NdeI CATATG 1 cut(s) 623
NlaIV GGNNCC 2 cut(s) 330, 659
NmuCI GTSAC 1 cut(s) 1360
NsbI TGCGCA 1 cut(s) 344
NsiI ATGCAT 1 cut(s) 1464
NspI RCATGY 1 cut(s) 670
PaeI GCATGC 1 cut(s) 670
PaeR7I CTCGAG 1 cut(s) 696
PagI TCATGA 1 cut(s) 1654
PdmI GAANNNNTTC 5 cut(s) 589, 1062, 1097, 1494, 1749
PflMI CCANNNNNTGG 2 cut(s) 1212, 2429
PkrI GCNGC 5 cut(s) 740, 1598, 2269, 2561, 2564
PleI GAGTC 6 cut(s) 191, 311, 1513, 1719, 1728, 1966
PpsI GAGTC 6 cut(s) 191, 311, 1513, 1719, 1728, 1966
Ppu21I YACGTR 2 cut(s) 542, 1193
PpuMI RGGWCCY 2 cut(s) 892, 2355
PshBI ATTAAT 1 cut(s) 2379
PsiI TTATAA 2 cut(s) 125, 1787
Psp5II RGGWCCY 2 cut(s) 892, 2355
Psp6I CCWGG 6 cut(s) 147, 245, 967, 1393, 1519, 2615
PspGI CCWGG 6 cut(s) 147, 245, 967, 1393, 1519, 2615
PspN4I GGNNCC 2 cut(s) 330, 659
PspPI GGNCC 5 cut(s) 170, 892, 965, 2355, 2623
PspPPI RGGWCCY 2 cut(s) 892, 2355
PspXI VCTCGAGB 1 cut(s) 696
PstNI CAGNNNCTG 1 cut(s) 2358
PsuI RGATCY 3 cut(s) 328, 657, 1621
RsaI GTAC 3 cut(s) 373, 1222, 1337
RsaNI GTAC 3 cut(s) 372, 1221, 1336
RseI CAYNNNNRTG 2 cut(s) 247, 584
SatI GCNGC 5 cut(s) 739, 1597, 2268, 2560, 2563
Sau96I GGNCC 5 cut(s) 170, 892, 965, 2355, 2623
SchI GAGTC 6 cut(s) 191, 312, 1514, 1720, 1729, 1967
ScrFI CCNGG 7 cut(s) 22, 149, 247, 969, 1395, 1521, 2617
SduI GDGCHC 1 cut(s) 106
SfaNI GCATC 4 cut(s) 1449, 1868, 2358, 2593
SfcI CTRYAG 1 cut(s) 2178
Sfr274I CTCGAG 1 cut(s) 696
SinI GGWCC 4 cut(s) 892, 965, 2355, 2623
SlaI CTCGAG 1 cut(s) 696
SmiMI CAYNNNNRTG 2 cut(s) 247, 584
SmlI CTYRAG 5 cut(s) 199, 696, 1415, 1610, 1715
SmoI CTYRAG 5 cut(s) 199, 696, 1415, 1610, 1715
SphI GCATGC 1 cut(s) 670
SsiI CCGC 6 cut(s) 73, 595, 639, 670, 1596, 2562
SspI AATATT 1 cut(s) 2087
SspMI CTAG 5 cut(s) 782, 945, 1292, 1373, 2109
StyD4I CCNGG 7 cut(s) 20, 147, 245, 967, 1393, 1519, 2615
StyI CCWWGG 1 cut(s) 661
TaaI ACNGT 2 cut(s) 376, 1455
TaiI ACGT 3 cut(s) 8, 544, 1195
TaqI TCGA 7 cut(s) 476, 507, 697, 1457, 1501, 1778, 2426
TatI WGTACW 2 cut(s) 1220, 1335
TauI GCSGC 2 cut(s) 1599, 2565
TscAI CASTG 2 cut(s) 2145, 2472
TseFI GTSAC 1 cut(s) 1360
TseI GCWGC 3 cut(s) 738, 2267, 2559
Tsp45I GTSAC 1 cut(s) 1360
TspGWI ACGGA 6 cut(s) 299, 336, 359, 510, 536, 1149
TspRI CASTG 2 cut(s) 2145, 2472
Van91I CCANNNNNTGG 2 cut(s) 1212, 2429
VneI GTGCAC 1 cut(s) 102
VpaK11BI GGWCC 4 cut(s) 892, 965, 2355, 2623
VspI ATTAAT 1 cut(s) 2379
XapI RAATTY 1 cut(s) 229
XbaI TCTAGA 1 cut(s) 2108
XceI RCATGY 1 cut(s) 670
XhoI CTCGAG 1 cut(s) 696
XmiI GTMKAC 1 cut(s) 538
XmnI GAANNNNTTC 5 cut(s) 589, 1062, 1097, 1494, 1749
XspI CTAG 5 cut(s) 782, 945, 1292, 1373, 2109
ZraI GACGTC 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.