Rroxscaffold_2G00146660

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000105
Physical Location & Seq
Reverse (-)
84508905 .. 84509980
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146660.1

Sequence Viewer

Length: 912 bp
ATGCCGTTGTCTTTATCTGATCTGTGTAGCTCTACAGAGCAAAGAATGAACAGATCACTGAGCAACCGGTTGCCTTTATTTGGTCTTTGTAATTTAACAGATCTAAACCTAAGTAACTGCAATCTTGGTGACACAGCATTTGCCAACAACTTCGGCTGCTTTCCCTCTTTACTGGCATTAAATCTCAGTGGAAATAATTTTTCTAAGCTTCCCCCAGGCATCAGATCATGTTTGAAACTAGAGAAGATTAACTTGGAGAACTGCATGAGTCTTCAAGAGTTGCCAGGCCTTCCTTCAAATAGTACATTGGATATAAGAGCTGATGGTTGTTCTTCACTGGAAATGTTGCTTCATGCATTCAATTTTGATAGATTGAGCATATCACATTTGAATTTCATCAACTGCTTCAAAATAAAGGACAATCAAGGCTGCAATAGCATATCATTTGAAATGCTGAAGGCATCTCTAATGCAGAGCAAACTTTTCAAGTTGTACTTCCTGGAAGGAAAATTCAAAATTCCCAGCTGGTTCAGCCATGAAAGGCCTGGAGTTTCGTTAAGTCTATGGCGAAGTCCAGATTTCGATTGCTGGGATACCAAATCTTTGATGGGTTTTGCTCTCTGTGTTGTGTTTAGACTCCATAAGCATCATCACATTAATGAACTCAACACAGATCAATTCAAGACATTTAAAGCTACACATCATCTGGTATGTTGCCTGAAAACCAATGGAAGAGAACAAGAAGTGTGTGGCAGACAGCCTGCGTTTCGCTTTAGTGAAGAATTTGCCGGGTTGAGTCACATCACATTTGGCTATTCTATGTTCGCAATCCCTGCGATTATTACTTTGGTACAGAGTGGAAGACTAGCAATCAGATTGAGTTCTTATTTGAAACCAAAGGGCCTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

303

Amino Acids

34.11

Weight (kDa)

8.74

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 172 - 239 5.9e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 4 cut(s) 391, 509, 516, 782
AcuI CTGAAG 1 cut(s) 476
AfaI GTAC 3 cut(s) 304, 494, 852
AfiI CCNNNNNNNGG 1 cut(s) 80
AgeI ACCGGT 1 cut(s) 66
AjnI CCWGG 5 cut(s) 214, 283, 498, 544, 903
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 5 cut(s) 30, 208, 320, 525, 695
AluI AGCT 5 cut(s) 30, 208, 320, 525, 695
AoxI GGCC 4 cut(s) 286, 542, 901, 906
ApeKI GCWGC 2 cut(s) 156, 429
ApoI RAATTY 4 cut(s) 391, 509, 516, 782
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AseI ATTAAT 1 cut(s) 657
AsiGI ACCGGT 1 cut(s) 66
AspS9I GGNCC 1 cut(s) 901
AsuC2I CCSGG 1 cut(s) 790
AsuHPI GGTGA 1 cut(s) 140
BbsI GAAGAC 2 cut(s) 263, 868
BbvI GCAGC 2 cut(s) 143, 416
BccI CCATC 2 cut(s) 317, 601
BciT130I CCWGG 5 cut(s) 216, 285, 500, 546, 905
BciVI GTATCC 1 cut(s) 586
BcnI CCSGG 1 cut(s) 790
BfaI CTAG 2 cut(s) 239, 866
BfmI CTRYAG 1 cut(s) 33
BfuI GTATCC 1 cut(s) 586
BglII AGATCT 1 cut(s) 100
BisI GCNGC 2 cut(s) 157, 430
BlsI GCNGC 2 cut(s) 158, 431
Bme1390I CCNGG 6 cut(s) 216, 285, 500, 546, 790, 905
BmgT120I GGNCC 1 cut(s) 901
BmrFI CCNGG 6 cut(s) 216, 285, 500, 546, 790, 905
BmsI GCATC 3 cut(s) 228, 470, 655
BpiI GAAGAC 2 cut(s) 263, 868
BpmI CTGGAG 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 790
BsaJI CCNNGG 1 cut(s) 214
BsaWI WCCGGW 1 cut(s) 66
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse118I RCCGGY 1 cut(s) 66
Bse1I ACTGG 2 cut(s) 177, 342
BseBI CCWGG 5 cut(s) 216, 285, 500, 546, 905
BseDI CCNNGG 1 cut(s) 214
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMII CTCAG 2 cut(s) 50, 199
BseNI ACTGG 2 cut(s) 177, 342
BseXI GCAGC 2 cut(s) 143, 416
BseYI CCCAGC 2 cut(s) 521, 588
BshFI GGCC 4 cut(s) 288, 544, 903, 908
BshTI ACCGGT 1 cut(s) 66
BsiSI CCGG 2 cut(s) 67, 789
BslI CCNNNNNNNGG 1 cut(s) 80
BsmI GAATGC 1 cut(s) 356
BsnI GGCC 4 cut(s) 288, 544, 903, 908
Bsp143I GATC 5 cut(s) 19, 53, 100, 224, 673
BspANI GGCC 4 cut(s) 288, 544, 903, 908
BspCNI CTCAG 2 cut(s) 51, 198
BsrFI RCCGGY 1 cut(s) 66
BsrI ACTGG 2 cut(s) 177, 342
BssAI RCCGGY 1 cut(s) 66
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 5 cut(s) 19, 53, 100, 224, 673
Bst2UI CCWGG 5 cut(s) 216, 285, 500, 546, 905
Bst6I CTCTTC 1 cut(s) 727
BstAPI GCANNNNNTGC 1 cut(s) 833
BstC8I GCNNGC 1 cut(s) 762
BstDEI CTNAG 4 cut(s) 59, 110, 185, 204
BstKTI GATC 5 cut(s) 22, 56, 103, 227, 676
BstMBI GATC 5 cut(s) 19, 53, 100, 224, 673
BstMWI GCNNNNNNNGC 3 cut(s) 435, 531, 833
BstNI CCWGG 5 cut(s) 216, 285, 500, 546, 905
BstSCI CCNGG 6 cut(s) 214, 283, 498, 544, 788, 903
BstSFI CTRYAG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 143, 416
BstV2I GAAGAC 2 cut(s) 263, 868
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuI GTATCC 1 cut(s) 586
BsuRI GGCC 4 cut(s) 288, 544, 903, 908
BtsIMutI CAGTG 3 cut(s) 56, 193, 335
Cac8I GCNNGC 1 cut(s) 762
Cfr10I RCCGGY 1 cut(s) 66
Cfr13I GGNCC 1 cut(s) 901
Csp6I GTAC 3 cut(s) 303, 493, 851
CspAI ACCGGT 1 cut(s) 66
CviAII CATG 4 cut(s) 228, 265, 353, 536
CviQI GTAC 3 cut(s) 303, 493, 851
DdeI CTNAG 4 cut(s) 59, 110, 185, 204
DpnI GATC 5 cut(s) 21, 55, 102, 226, 675
DpnII GATC 5 cut(s) 19, 53, 100, 224, 673
DraI TTTAAA 1 cut(s) 691
Eam1104I CTCTTC 1 cut(s) 727
EarI CTCTTC 1 cut(s) 727
Eco147I AGGCCT 2 cut(s) 288, 544
Eco57I CTGAAG 1 cut(s) 476
EcoO109I RGGNCCY 1 cut(s) 901
EcoRII CCWGG 5 cut(s) 214, 283, 498, 544, 903
EcoT22I ATGCAT 1 cut(s) 358
FaeI CATG 4 cut(s) 231, 268, 356, 539
FalI AAGNNNNNCTT 4 cut(s) 236, 268, 479, 511
FatI CATG 4 cut(s) 227, 264, 352, 535
Fnu4HI GCNGC 2 cut(s) 157, 430
Fsp4HI GCNGC 2 cut(s) 157, 430
FspBI CTAG 2 cut(s) 239, 866
GluI GCNGC 2 cut(s) 157, 430
GsaI CCCAGC 2 cut(s) 525, 592
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 4 cut(s) 288, 544, 903, 908
HapII CCGG 2 cut(s) 67, 789
Hin1II CATG 4 cut(s) 231, 268, 356, 539
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 3 cut(s) 268, 636, 796
HpaII CCGG 2 cut(s) 67, 789
HphI GGTGA 1 cut(s) 140
Hpy188I TCNGA 3 cut(s) 19, 224, 875
Hpy188III TCNNGA 3 cut(s) 275, 575, 682
HpyAV CCTTC 4 cut(s) 299, 303, 451, 497
HpyCH4V TGCA 5 cut(s) 120, 264, 356, 432, 472
HpyF10VI GCNNNNNNNGC 3 cut(s) 435, 531, 833
HpyF3I CTNAG 4 cut(s) 59, 110, 185, 204
Hsp92II CATG 4 cut(s) 231, 268, 356, 539
Kzo9I GATC 5 cut(s) 19, 53, 100, 224, 673
Lsp1109I GCAGC 2 cut(s) 143, 416
LweI GCATC 3 cut(s) 228, 470, 655
MaeI CTAG 2 cut(s) 239, 866
MaeIII GTNAC 3 cut(s) 113, 128, 797
MalI GATC 5 cut(s) 21, 55, 102, 226, 675
MboI GATC 5 cut(s) 19, 53, 100, 224, 673
MboII GAAGA 6 cut(s) 256, 263, 324, 744, 791, 873
MflI RGATCY 1 cut(s) 100
MluCI AATT 8 cut(s) 91, 196, 361, 391, 509, 516, 677, 782
MlyI GAGTC 3 cut(s) 277, 630, 805
MnlI CCTC 1 cut(s) 175
Mph1103I ATGCAT 1 cut(s) 358
MseI TTAA 6 cut(s) 95, 179, 249, 557, 657, 690
MslI CAYNNNNRTG 1 cut(s) 657
MspA1I CMGCKG 1 cut(s) 525
MspI CCGG 2 cut(s) 67, 789
MspR9I CCNGG 6 cut(s) 216, 285, 500, 546, 790, 905
Mva1269I GAATGC 1 cut(s) 356
MvaI CCWGG 5 cut(s) 216, 285, 500, 546, 905
MwoI GCNNNNNNNGC 3 cut(s) 435, 531, 833
NciI CCSGG 1 cut(s) 790
NdeII GATC 5 cut(s) 19, 53, 100, 224, 673
NlaIII CATG 4 cut(s) 231, 268, 356, 539
NmuCI GTSAC 2 cut(s) 128, 797
NsiI ATGCAT 1 cut(s) 358
PceI AGGCCT 2 cut(s) 288, 544
PctI GAATGC 1 cut(s) 356
PfoI TCCNGGA 1 cut(s) 498
PinAI ACCGGT 1 cut(s) 66
PkrI GCNGC 2 cut(s) 158, 431
PleI GAGTC 3 cut(s) 276, 630, 804
PpsI GAGTC 3 cut(s) 276, 630, 804
PshBI ATTAAT 1 cut(s) 657
Psp6I CCWGG 5 cut(s) 214, 283, 498, 544, 903
PspFI CCCAGC 2 cut(s) 521, 588
PspGI CCWGG 5 cut(s) 214, 283, 498, 544, 903
PspPI GGNCC 1 cut(s) 901
PsuI RGATCY 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 525
RsaI GTAC 3 cut(s) 304, 494, 852
RsaNI GTAC 3 cut(s) 303, 493, 851
RseI CAYNNNNRTG 1 cut(s) 657
SaqAI TTAA 6 cut(s) 95, 179, 249, 557, 657, 690
SatI GCNGC 2 cut(s) 157, 430
Sau3AI GATC 5 cut(s) 19, 53, 100, 224, 673
Sau96I GGNCC 1 cut(s) 901
SchI GAGTC 3 cut(s) 277, 630, 805
ScrFI CCNGG 6 cut(s) 216, 285, 500, 546, 790, 905
SetI ASST 6 cut(s) 32, 111, 210, 322, 527, 697
SfaNI GCATC 3 cut(s) 228, 470, 655
SfcI CTRYAG 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 657
Sse9I AATT 8 cut(s) 91, 196, 361, 391, 509, 516, 677, 782
SseBI AGGCCT 2 cut(s) 288, 544
SspMI CTAG 2 cut(s) 239, 866
StuI AGGCCT 2 cut(s) 288, 544
StyD4I CCNGG 6 cut(s) 214, 283, 498, 544, 788, 903
TaqI TCGA 1 cut(s) 582
TasI AATT 8 cut(s) 91, 196, 361, 391, 509, 516, 677, 782
TatI WGTACW 2 cut(s) 302, 492
Tru1I TTAA 6 cut(s) 95, 179, 249, 557, 657, 690
Tru9I TTAA 6 cut(s) 95, 179, 249, 557, 657, 690
TscAI CASTG 3 cut(s) 63, 193, 342
TseFI GTSAC 2 cut(s) 128, 797
TseI GCWGC 2 cut(s) 156, 429
Tsp45I GTSAC 2 cut(s) 128, 797
TspDTI ATGAA 5 cut(s) 62, 341, 385, 552, 675
TspRI CASTG 3 cut(s) 63, 193, 342
VspI ATTAAT 1 cut(s) 657
XapI RAATTY 4 cut(s) 391, 509, 516, 782
XcmI CCANNNNNNNNNTGG 2 cut(s) 542, 604
XspI CTAG 2 cut(s) 239, 866
Zsp2I ATGCAT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.