RchiOBHm_Chr1g0329001

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
18455650 .. 18464236
8587 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55835

Sequence Viewer

Length: 3774 bp
ATGGCGTCAACCTCTTCTTGTCGTCAATGTAAATACGATGTCTTTCTAAGTTTCAGGGGGACAGATACCCGCATGGGTTTTACCGGTCATCTGTATTCTGCACTAATTCAGAGAGGGATTCTCACCTTTCGGGATGACCCAGGGCTTGAGAGAGGAAGATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGAGTCCAGATGTGCGATTGTTATTCTCTCATGGAACTATGCTTCTTCGTCATGGTGCTTGGACGAACTCGTCAAGATTATTCAATGCATGAAAGAGAGGGGCCAACAAGTCTTCCCCGTCTTCTTAAATGGCGCGGATCCTTCTGATGTGCGTCACCAAAGGGGACATTTTGAGCTCAAAAGCAAAGCCAAAGTAGATGTGGAAGTAAGGGAGCATGAAGAAGTTTATGGGCAGAATAATGAGGACAGAGTAAATGCGTGGAGAGCTGCTTTGACACAGGTCGCCAATCTTTCTGGCTGGGATGCCAAGAAGTATTCGTTCACTGAAACAAGAATAATTGAGGAAATTATTAATCATATACTAAAGAACTCCGTGTATACATCTGCAAGTGTTGATAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTATTAAGCAATTACATATGTCCGCAGCTTGGTGGGGTGCGGTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATTCATGATGAAATTCGTCAGGATTTTGACCGAGCCTACTTTCTTTCCAATGTCAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTTCAAGAAAAACTTCTTTCCGAAATCCTCATGGCAAAAATTAAAAATATAGAGCATGAAGACACAGGAGCTGCTATGATAAAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATTGTCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGGTGTTGATGCTACATACAGAGCTAACGGGTTAAACTGTGATGAATCACTTCAACTTTTGTGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGCATCTGTGCTACAATATTTTAGGGTATGCTAAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTTTATTCGGTAGAAGTACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGATGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTACATATTGCTTGCTTTTACAGGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAGTTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAAAAATATGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTGGACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTTGTAATGTGGACCTTCCTGACGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCTTTGAGATCTCTCCCACAAAACTTTGAAGCAGATGAACTTGTTGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCACAACATTGTGGAGACCCCAGACCTTGCAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGTTAAGAGTCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAGAGTCTCGAGAGTCTGCCAAGTATAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAATAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTCGGCGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGTGTCAATTGAACGGCTGAGTGGCCTTCTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTCTGTCTTCAGAGCACCATCAATAAATTGAAGTCTATTGAAGATCTTAATCTTTCTGGATGCTTGAACCTCGGCAAACCTCAGGTAAATGTGGGGGTGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCGTCTACCCATGATCGCAAGAAAAAAGGGAGAGGTCCAATCTTTCAAGGATGCAAAGTAGTTTGGCGATCTTTCAAAAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATATAGAGCCAAAGAGCTTCCACTTGCCTATATCACAAAACGTGAATGCAGAGCCAAAGAGTTTCCACCGGCCTATATCCCAAAAAGTGAATGCAGAGCCAAAGAGTTTCCACTCGCCTATATCACAAAAAGTGAATGCAGAGCCAAAGAGCTTCCACTCGCCTATATCACAAAAAGTAAATACAGAGCCAATGAGTTTCCCCTTGCCTATATCTGGTCTGTGTAATTTAATAAATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGGAACAATTTTGTTCGTCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAAACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAACAGCAATCAAGGATGCAATAACATAGCATTCGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAGCAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCTTGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCTGTAAGTGTATCTCTACCTGCACATTGGAATAACAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTCCATGAGCACCTTCAGGTGGATAAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTTGATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGTAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCGTAATAGTTGCAGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATGTGCAGGAGTTGAACAAAACAACCACTCAATCAAGTAGTGGGATGTCTCCTTACGAGGATATATTGATTAGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGGCAGTGGTAGCAGAACTTGCACGCTTGAAGAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1257

Amino Acids

142.09

Weight (kDa)

6.43

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 193 3.2e-44 TIR domain
TIR_2 PF13676 14 - 123 1.7e-12 TIR domain
NB-ARC PF00931 220 - 374 1.4e-19 NB-ARC domain
NACHT PF05729 224 - 321 2.8e-07 NACHT domain
WHD_ROQ1 PF23282 441 - 512 2.7e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 610 - 628 5.3e-07 Leucine Rich Repeat
LRR_14 PF23598 679 - 780 5.3e-09 Leucine-rich repeat region
LRR_13 PF23286 701 - 785 1.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1064 - 1213 3.5e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3772
AasI GACNNNNNNGTC 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 3268
AccB7I CCANNNNNTGG 1 cut(s) 1630
AccI GTMKAC 3 cut(s) 569, 2438, 2983
AccII CGCG 1 cut(s) 326
AciI CCGC 7 cut(s) 70, 326, 613, 647, 664, 688, 895
AclI AACGTT 1 cut(s) 2176
AclWI GGATC 7 cut(s) 153, 323, 336, 670, 683, 945, 1389
AcsI RAATTY 8 cut(s) 732, 1687, 1885, 1996, 2828, 3040, 3356, 3461
AcuI CTGAAG 7 cut(s) 1616, 2276, 2877, 3155, 3314, 3419, 3611
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 4 cut(s) 1231, 3010, 3315, 3526
AfiI CCNNNNNNNGG 6 cut(s) 653, 1427, 1630, 2759, 3334, 3722
AgeI ACCGGT 1 cut(s) 83
AjnI CCWGG 5 cut(s) 139, 988, 1546, 3193, 3583
AjuI GAANNNNNNNTTGG 2 cut(s) 2915, 2947
Alw21I GWGCWC 3 cut(s) 369, 2300, 3327
Alw26I GTCTC 9 cut(s) 1539, 1575, 1698, 1759, 1931, 2075, 2195, 2941, 3684
AlwI GGATC 7 cut(s) 153, 323, 336, 670, 683, 945, 1389
AlwNI CAGNNNCTG 1 cut(s) 878
Ama87I CYCGRG 3 cut(s) 714, 2072, 2153
AoxI GGCC 7 cut(s) 167, 292, 796, 1738, 2245, 2615, 3581
ApeKI GCWGC 4 cut(s) 182, 458, 649, 878
ApoI RAATTY 8 cut(s) 732, 1687, 1885, 1996, 2828, 3040, 3356, 3461
AseI ATTAAT 2 cut(s) 543, 2117
AsiGI ACCGGT 1 cut(s) 83
Asp700I GAANNNNTTC 7 cut(s) 607, 819, 1089, 1521, 1615, 3140, 3177
AspLEI GCGC 2 cut(s) 326, 2188
AspS9I GGNCC 5 cut(s) 292, 986, 1726, 2468, 3581
AsuHPI GGTGA 7 cut(s) 115, 338, 2392, 2819, 2863, 3476, 3607
AsuII TTCGAA 1 cut(s) 3126
AvaI CYCGRG 3 cut(s) 714, 2072, 2153
AvaII GGWCC 3 cut(s) 986, 1726, 2468
AxyI CCTNAGG 1 cut(s) 2364
BamHI GGATCC 2 cut(s) 328, 675
BanII GRGCYC 1 cut(s) 369
BauI CACGAG 2 cut(s) 1196, 3507
BbsI GAAGAC 8 cut(s) 295, 304, 873, 1584, 1697, 2282, 2879, 3143
Bbv12I GWGCWC 3 cut(s) 369, 2300, 3327
BbvI GCAGC 4 cut(s) 169, 445, 661, 865
BccI CCATC 8 cut(s) 1307, 2019, 2309, 2377, 2980, 2996, 3231, 3401
BceAI ACGGC 2 cut(s) 1753, 2252
BcgI CGANNNNNNTGC 2 cut(s) 2053, 2087
BciT130I CCWGG 5 cut(s) 141, 990, 1548, 3195, 3585
BciVI GTATCC 1 cut(s) 1797
BcoDI GTCTC 9 cut(s) 1539, 1575, 1698, 1759, 1931, 2075, 2195, 2941, 3684
BfaI CTAG 3 cut(s) 800, 1400, 2987
BfmI CTRYAG 1 cut(s) 2272
BfoI RGCGCY 1 cut(s) 2189
BfuAI ACCTGC 1 cut(s) 3268
BfuI GTATCC 1 cut(s) 1797
BglII AGATCT 4 cut(s) 1451, 1796, 1978, 2326
BisI GCNGC 4 cut(s) 183, 459, 650, 879
BlsI GCNGC 4 cut(s) 184, 460, 651, 880
BmcAI AGTACT 1 cut(s) 1231
Bme1390I CCNGG 5 cut(s) 141, 990, 1548, 3195, 3585
Bme18I GGWCC 3 cut(s) 986, 1726, 2468
BmeT110I CYCGRG 3 cut(s) 714, 2072, 2153
BmgT120I GGNCC 5 cut(s) 292, 986, 1726, 2468, 3581
BmiI GGNNCC 3 cut(s) 293, 330, 677
BmrFI CCNGG 5 cut(s) 141, 990, 1548, 3195, 3585
BmrI ACTGGG 1 cut(s) 1791
BmsI GCATC 9 cut(s) 484, 1039, 1156, 1476, 2333, 2474, 3022, 3044, 3098
BmuI ACTGGG 1 cut(s) 1791
BoxI GACNNNNGTC 1 cut(s) 470
BpiI GAAGAC 8 cut(s) 295, 304, 873, 1584, 1697, 2282, 2879, 3143
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu10I CCTNAGC 1 cut(s) 1176
Bpu14I TTCGAA 1 cut(s) 3126
BpuEI CTTGAG 5 cut(s) 167, 1783, 1988, 2939, 3230
BsaBI GATNNNNATC 3 cut(s) 936, 1380, 2331
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1575, 1931
BsaJI CCNNGG 7 cut(s) 139, 140, 679, 989, 1624, 2020, 2353
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 6 cut(s) 653, 1427, 1630, 2759, 3334, 3722
Bse118I RCCGGY 2 cut(s) 83, 2613
Bse1I ACTGG 5 cut(s) 983, 1323, 1786, 3716, 3732
Bse21I CCTNAGG 1 cut(s) 2364
Bse8I GATNNNNATC 3 cut(s) 936, 1380, 2331
BseBI CCWGG 5 cut(s) 141, 990, 1548, 3195, 3585
BseDI CCNNGG 7 cut(s) 139, 140, 679, 989, 1624, 2020, 2353
BseGI GGATG 9 cut(s) 139, 499, 1327, 1491, 1994, 2348, 2489, 3113, 3681
BseJI GATNNNNATC 3 cut(s) 936, 1380, 2331
BseLI CCNNNNNNNGG 6 cut(s) 653, 1427, 1630, 2759, 3334, 3722
BseMII CTCAG 3 cut(s) 2231, 2378, 2780
BseNI ACTGG 5 cut(s) 983, 1323, 1786, 3716, 3732
BseRI GAGGAG 2 cut(s) 206, 1760
BseXI GCAGC 4 cut(s) 169, 445, 661, 865
BseYI CCCAGC 2 cut(s) 489, 1512
BsgI GTGCAG 3 cut(s) 84, 3246, 3656
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 7 cut(s) 169, 294, 798, 1740, 2247, 2617, 3583
BshTI ACCGGT 1 cut(s) 83
BsiHKAI GWGCWC 3 cut(s) 369, 2300, 3327
BsiHKCI CYCGRG 3 cut(s) 714, 2072, 2153
BsiSI CCGG 2 cut(s) 84, 2614
BslFI GGGAC 3 cut(s) 73, 369, 1110
BslI CCNNNNNNNGG 6 cut(s) 653, 1427, 1630, 2759, 3334, 3722
BsmAI GTCTC 9 cut(s) 1539, 1575, 1698, 1759, 1931, 2075, 2195, 2941, 3684
BsmFI GGGAC 3 cut(s) 73, 369, 1110
BsmI GAATGC 4 cut(s) 2596, 2641, 2686, 3122
BsnI GGCC 7 cut(s) 169, 294, 798, 1740, 2247, 2617, 3583
Bso31I GGTCTC 2 cut(s) 1575, 1931
BsoBI CYCGRG 3 cut(s) 714, 2072, 2153
Bsp119I TTCGAA 1 cut(s) 3126
Bsp1286I GDGCHC 3 cut(s) 369, 2300, 3327
Bsp19I CCATGG 1 cut(s) 679
BspACI CCGC 7 cut(s) 70, 326, 613, 647, 664, 688, 895
BspANI GGCC 7 cut(s) 169, 294, 798, 1740, 2247, 2617, 3583
BspCNI CTCAG 3 cut(s) 2232, 2377, 2781
BspFNI CGCG 1 cut(s) 326
BspHI TCATGA 2 cut(s) 724, 1573
BspLI GGNNCC 3 cut(s) 293, 330, 677
BspMAI CTGCAG 1 cut(s) 2276
BspMI ACCTGC 1 cut(s) 3268
BspPI GGATC 7 cut(s) 153, 323, 336, 670, 683, 945, 1389
BspT104I TTCGAA 1 cut(s) 3126
BspTNI GGTCTC 2 cut(s) 1575, 1931
BsrFI RCCGGY 2 cut(s) 83, 2613
BsrI ACTGG 5 cut(s) 983, 1323, 1786, 3716, 3732
BssAI RCCGGY 2 cut(s) 83, 2613
BssECI CCNNGG 7 cut(s) 139, 140, 679, 989, 1624, 2020, 2353
BssNAI GTATAC 2 cut(s) 570, 2984
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1196, 3507
BssT1I CCWWGG 3 cut(s) 679, 1624, 2020
Bst1107I GTATAC 2 cut(s) 570, 2984
Bst2BI CACGAG 2 cut(s) 1196, 3507
Bst2UI CCWGG 5 cut(s) 141, 990, 1548, 3195, 3585
Bst4CI ACNGT 5 cut(s) 1079, 1482, 1533, 2390, 3278
Bst6I CTCTTC 3 cut(s) 19, 813, 3406
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 3 cut(s) 1495, 3634, 3753
BstBI TTCGAA 1 cut(s) 3126
BstC8I GCNNGC 6 cut(s) 686, 1357, 1412, 1954, 3441, 3758
BstDEI CTNAG 9 cut(s) 47, 1176, 1700, 2240, 2264, 2364, 2789, 2864, 2913
BstDSI CCRYGG 1 cut(s) 679
BstEII GGTNACC 1 cut(s) 2851
BstF5I GGATG 9 cut(s) 139, 499, 1327, 1491, 1994, 2348, 2489, 3113, 3681
BstFNI CGCG 1 cut(s) 326
BstH2I RGCGCY 1 cut(s) 2189
BstHHI GCGC 2 cut(s) 326, 2188
BstMAI GTCTC 9 cut(s) 1539, 1575, 1698, 1759, 1931, 2075, 2195, 2941, 3684
BstMWI GCNNNNNNNGC 8 cut(s) 455, 690, 1495, 2914, 3302, 3634, 3744, 3753
BstNI CCWGG 5 cut(s) 141, 990, 1548, 3195, 3585
BstNSI RCATGY 1 cut(s) 688
BstPAI GACNNNNGTC 1 cut(s) 470
BstPI GGTNACC 1 cut(s) 2851
BstSCI CCNGG 5 cut(s) 139, 988, 1546, 3193, 3583
BstSFI CTRYAG 1 cut(s) 2272
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 4 cut(s) 169, 445, 661, 865
BstV2I GAAGAC 8 cut(s) 295, 304, 873, 1584, 1697, 2282, 2879, 3143
BstX2I RGATCY 7 cut(s) 158, 328, 675, 1451, 1796, 1978, 2326
BstYI RGATCY 7 cut(s) 158, 328, 675, 1451, 1796, 1978, 2326
BstZ17I GTATAC 2 cut(s) 570, 2984
Bsu36I CCTNAGG 1 cut(s) 2364
BsuI GTATCC 1 cut(s) 1797
BsuRI GGCC 7 cut(s) 169, 294, 798, 1740, 2247, 2617, 3583
BtgI CCRYGG 1 cut(s) 679
BtsCI GGATG 9 cut(s) 139, 499, 1327, 1491, 1994, 2348, 2489, 3113, 3681
BtsI GCAGTG 1 cut(s) 3745
BtsIMutI CAGTG 4 cut(s) 513, 3014, 3739, 3745
BveI ACCTGC 1 cut(s) 3268
Cac8I GCNNGC 6 cut(s) 686, 1357, 1412, 1954, 3441, 3758
CaiI CAGNNNCTG 1 cut(s) 878
CciI TCATGA 2 cut(s) 724, 1573
CfoI GCGC 2 cut(s) 326, 2188
Cfr10I RCCGGY 2 cut(s) 83, 2613
Cfr13I GGNCC 5 cut(s) 292, 986, 1726, 2468, 3581
CseI GACGC 1 cut(s) 332
Csp6I GTAC 4 cut(s) 1230, 3009, 3314, 3525
CspAI ACCGGT 1 cut(s) 83
CviQI GTAC 4 cut(s) 1230, 3009, 3314, 3525
DdeI CTNAG 9 cut(s) 47, 1176, 1700, 2240, 2264, 2364, 2789, 2864, 2913
DraIII CACNNNGTG 1 cut(s) 1043
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
Eam1104I CTCTTC 3 cut(s) 19, 813, 3406
EarI CTCTTC 3 cut(s) 19, 813, 3406
Ecl136II GAGCTC 1 cut(s) 367
Eco130I CCWWGG 3 cut(s) 679, 1624, 2020
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 2 cut(s) 1575, 1931
Eco47I GGWCC 3 cut(s) 986, 1726, 2468
Eco53kI GAGCTC 1 cut(s) 367
Eco57I CTGAAG 7 cut(s) 1616, 2276, 2877, 3155, 3314, 3419, 3611
Eco81I CCTNAGG 1 cut(s) 2364
Eco88I CYCGRG 3 cut(s) 714, 2072, 2153
Eco91I GGTNACC 1 cut(s) 2851
EcoICRI GAGCTC 1 cut(s) 367
EcoO65I GGTNACC 1 cut(s) 2851
EcoRI GAATTC 1 cut(s) 3356
EcoRII CCWGG 5 cut(s) 139, 988, 1546, 3193, 3583
EcoT14I CCWWGG 3 cut(s) 679, 1624, 2020
EcoT22I ATGCAT 3 cut(s) 281, 1491, 3037
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 3 cut(s) 679, 1624, 2020
FalI AAGNNNNNCTT 2 cut(s) 804, 836
FaqI GGGAC 3 cut(s) 73, 369, 1110
FauI CCCGC 3 cut(s) 77, 620, 681
FauNDI CATATG 1 cut(s) 641
FblI GTMKAC 3 cut(s) 569, 2438, 2983
Fnu4HI GCNGC 4 cut(s) 183, 459, 650, 879
FokI GGATG 9 cut(s) 146, 506, 1334, 1498, 2001, 2355, 2496, 3120, 3688
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 4 cut(s) 183, 459, 650, 879
FspBI CTAG 3 cut(s) 800, 1400, 2987
GlaI GCGC 2 cut(s) 325, 2187
GluI GCNGC 4 cut(s) 183, 459, 650, 879
GsaI CCCAGC 2 cut(s) 493, 1516
HaeII RGCGCY 1 cut(s) 2189
HaeIII GGCC 7 cut(s) 169, 294, 798, 1740, 2247, 2617, 3583
HapII CCGG 2 cut(s) 84, 2614
HgaI GACGC 1 cut(s) 332
HhaI GCGC 2 cut(s) 326, 2188
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 324, 2186
HinP1I GCGC 2 cut(s) 324, 2186
HincII GTYRAC 2 cut(s) 9, 945
HindII GTYRAC 2 cut(s) 9, 945
HindIII AAGCTT 4 cut(s) 2036, 2915, 3341, 3401
HpaII CCGG 2 cut(s) 84, 2614
HphI GGTGA 7 cut(s) 115, 338, 2392, 2819, 2863, 3476, 3607
Hpy166II GTNNAC 9 cut(s) 9, 513, 570, 945, 1529, 1644, 1726, 2439, 2984
Hpy8I GTNNAC 9 cut(s) 9, 513, 570, 945, 1529, 1644, 1726, 2439, 2984
HpyCH4III ACNGT 5 cut(s) 1079, 1482, 1533, 2390, 3278
HpyCH4IV ACGT 3 cut(s) 1555, 2176, 2586
HpyF10VI GCNNNNNNNGC 8 cut(s) 455, 690, 1495, 2914, 3302, 3634, 3744, 3753
HpyF3I CTNAG 9 cut(s) 47, 1176, 1700, 2240, 2264, 2364, 2789, 2864, 2913
HpySE526I ACGT 3 cut(s) 1555, 2176, 2586
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 324, 2186
LmnI GCTCC 3 cut(s) 403, 875, 2813
Lsp1109I GCAGC 4 cut(s) 169, 445, 661, 865
LweI GCATC 9 cut(s) 484, 1039, 1156, 1476, 2333, 2474, 3022, 3044, 3098
MaeI CTAG 3 cut(s) 800, 1400, 2987
MaeII ACGT 3 cut(s) 1555, 2176, 2586
MaeIII GTNAC 4 cut(s) 344, 1387, 2834, 2851
MfeI CAATTG 3 cut(s) 186, 2229, 3079
MflI RGATCY 7 cut(s) 158, 328, 675, 1451, 1796, 1978, 2326
MhlI GDGCHC 3 cut(s) 369, 2300, 3327
MlyI GAGTC 6 cut(s) 203, 1761, 2016, 2077, 2086, 3618
MmeI TCCRAC 2 cut(s) 1494, 2143
Mph1103I ATGCAT 3 cut(s) 281, 1491, 3037
MroXI GAANNNNTTC 7 cut(s) 607, 819, 1089, 1521, 1615, 3140, 3177
MslI CAYNNNNRTG 3 cut(s) 602, 1634, 3228
MspI CCGG 2 cut(s) 84, 2614
MspR9I CCNGG 5 cut(s) 141, 990, 1548, 3195, 3585
MunI CAATTG 3 cut(s) 186, 2229, 3079
Mva1269I GAATGC 4 cut(s) 2596, 2641, 2686, 3122
MvaI CCWGG 5 cut(s) 141, 990, 1548, 3195, 3585
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 8 cut(s) 455, 690, 1495, 2914, 3302, 3634, 3744, 3753
NcoI CCATGG 1 cut(s) 679
NdeI CATATG 1 cut(s) 641
NlaIV GGNNCC 3 cut(s) 293, 330, 677
NmeAIII GCCGAG 1 cut(s) 2334
NmuCI GTSAC 3 cut(s) 344, 1387, 2851
NsiI ATGCAT 3 cut(s) 281, 1491, 3037
NspI RCATGY 1 cut(s) 688
NspV TTCGAA 1 cut(s) 3126
PaeI GCATGC 1 cut(s) 688
PaeR7I CTCGAG 2 cut(s) 714, 2072
PagI TCATGA 2 cut(s) 724, 1573
PasI CCCWGGG 1 cut(s) 140
PceI AGGCCT 1 cut(s) 169
PctI GAATGC 4 cut(s) 2596, 2641, 2686, 3122
PdmI GAANNNNTTC 7 cut(s) 607, 819, 1089, 1521, 1615, 3140, 3177
PflMI CCANNNNNTGG 1 cut(s) 1630
PfoI TCCNGGA 1 cut(s) 3193
PinAI ACCGGT 1 cut(s) 83
PkrI GCNGC 4 cut(s) 184, 460, 651, 880
PleI GAGTC 6 cut(s) 202, 1761, 2015, 2076, 2085, 3617
PpsI GAGTC 6 cut(s) 202, 1761, 2015, 2076, 2085, 3617
PshAI GACNNNNGTC 1 cut(s) 470
PshBI ATTAAT 2 cut(s) 543, 2117
PsiI TTATAA 1 cut(s) 3772
Psp124BI GAGCTC 1 cut(s) 369
Psp1406I AACGTT 1 cut(s) 2176
Psp6I CCWGG 5 cut(s) 139, 988, 1546, 3193, 3583
PspEI GGTNACC 1 cut(s) 2851
PspFI CCCAGC 2 cut(s) 489, 1512
PspGI CCWGG 5 cut(s) 139, 988, 1546, 3193, 3583
PspN4I GGNNCC 3 cut(s) 293, 330, 677
PspPI GGNCC 5 cut(s) 292, 986, 1726, 2468, 3581
PspXI VCTCGAGB 1 cut(s) 714
PstI CTGCAG 1 cut(s) 2276
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 7 cut(s) 158, 328, 675, 1451, 1796, 1978, 2326
RsaI GTAC 4 cut(s) 1231, 3010, 3315, 3526
RsaNI GTAC 4 cut(s) 1230, 3009, 3314, 3525
RseI CAYNNNNRTG 3 cut(s) 602, 1634, 3228
SacI GAGCTC 1 cut(s) 369
SatI GCNGC 4 cut(s) 183, 459, 650, 879
Sau96I GGNCC 5 cut(s) 292, 986, 1726, 2468, 3581
ScaI AGTACT 1 cut(s) 1231
SchI GAGTC 6 cut(s) 203, 1761, 2016, 2077, 2086, 3618
ScrFI CCNGG 5 cut(s) 141, 990, 1548, 3195, 3585
SduI GDGCHC 3 cut(s) 369, 2300, 3327
SfaNI GCATC 9 cut(s) 484, 1039, 1156, 1476, 2333, 2474, 3022, 3044, 3098
SfcI CTRYAG 1 cut(s) 2272
Sfr274I CTCGAG 2 cut(s) 714, 2072
SfuI TTCGAA 1 cut(s) 3126
SinI GGWCC 3 cut(s) 986, 1726, 2468
SlaI CTCGAG 2 cut(s) 714, 2072
SmiMI CAYNNNNRTG 3 cut(s) 602, 1634, 3228
SmlI CTYRAG 7 cut(s) 146, 714, 1762, 1967, 2072, 2918, 3209
SmoI CTYRAG 7 cut(s) 146, 714, 1762, 1967, 2072, 2918, 3209
SphI GCATGC 1 cut(s) 688
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 7 cut(s) 70, 326, 613, 647, 664, 688, 895
SspI AATATT 3 cut(s) 1162, 1341, 3205
SspMI CTAG 3 cut(s) 800, 1400, 2987
SstI GAGCTC 1 cut(s) 369
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 5 cut(s) 139, 988, 1546, 3193, 3583
StyI CCWWGG 3 cut(s) 679, 1624, 2020
TaaI ACNGT 5 cut(s) 1079, 1482, 1533, 2390, 3278
TaiI ACGT 3 cut(s) 1558, 2179, 2589
TaqI TCGA 4 cut(s) 715, 1773, 2073, 3126
TaqII GACCGA 1 cut(s) 765
TatI WGTACW 3 cut(s) 1229, 3008, 3313
TscAI CASTG 4 cut(s) 520, 3021, 3739, 3745
TseFI GTSAC 3 cut(s) 344, 1387, 2851
TseI GCWGC 4 cut(s) 182, 458, 649, 878
Tsp45I GTSAC 3 cut(s) 344, 1387, 2851
TspGWI ACGGA 3 cut(s) 553, 1176, 3602
TspRI CASTG 4 cut(s) 520, 3021, 3739, 3745
Van91I CCANNNNNTGG 1 cut(s) 1630
VpaK11BI GGWCC 3 cut(s) 986, 1726, 2468
VspI ATTAAT 2 cut(s) 543, 2117
XapI RAATTY 8 cut(s) 732, 1687, 1885, 1996, 2828, 3040, 3356, 3461
XceI RCATGY 1 cut(s) 688
XcmI CCANNNNNNNNNTGG 3 cut(s) 388, 2018, 3230
XhoI CTCGAG 2 cut(s) 714, 2072
XmiI GTMKAC 3 cut(s) 569, 2438, 2983
XmnI GAANNNNTTC 7 cut(s) 607, 819, 1089, 1521, 1615, 3140, 3177
XspI CTAG 3 cut(s) 800, 1400, 2987
ZrmI AGTACT 1 cut(s) 1231
Zsp2I ATGCAT 3 cut(s) 281, 1491, 3037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.