Rmu_sc0000252.1_g000020

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000252.1
OG0000105
Physical Location & Seq
Forward (+)
103687 .. 108204
4518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000252.1_g000020.1.cds

Sequence Viewer

Length: 3426 bp
atggcgtcaacctcttctcgtcgtcaatggaaatacgatgtgtttctaaatttcaggggggaagatacccgcaagggttttactggtcgcctgtattctgcactaaatcagagaggaattctcacctttatggatgatgcagagcttgagaaaggaaaatccattaggcctgaacttttagctgcaattgaggactctagatccgcgattgtcattctctcatggaactatgctcattcgtcatggtgcttggatgaactcgtcaagattattcaatgcatgaaaaagatgggccaacaagtcctccccgtcttctacaaagtggatccttctgatgtgcggcaccaaaggcgaagttttgagctcaaagtaagggaaccccaagtagaagtaagggaacatgaagaagtttatgggaagcttaatgaggacagactaaatgcctggagagctgctttgactgaggtcgccaatctttctggctggcatttcaagaatgcttcatctcttacaaaactaactgaggaaattgttaatcatatactaaagaagtccgtatatacatcttcaagtgttgacaagggcttcataggaatgggttcccgcattcatgatttattaagcaattacgtatgcccgcacctttgcggggtgcgttttatagggatccatagcatgcggggcataggtaagacaacacttgctcgagctatccatgatgaaatccgtcaggattttgaccgaacctgctttctttccaatgttagagaaatgtctaagaagaatggcctagtttctctacaagaaaaacttctttccagaatcctgctggcaaaatttaaaaatatagagaatgaagacataggagctgctatgatagaaaggcggttgtgtagaagaaaggtgcttgttgttattgatgatgtggatcagttgatacaattagagaaattggctggagggcgcagctggtttggtccagggagtagaatcatcataaccaccacagatattcagttgttaaaggcacatgatgttgatgctacatacaaggctaacgggttaaactgttatgaagcacttcaacttttgagtttgaaggcttttaagaaatgtcccccaccagtagattatttgcatttgtgcaaccttattttagggtatgctcgggggcttccgttggctctagtggttttaggttcttttctatttggtagaagcactgttgaatgggcaagtgcaatagataggctaaagaacacaccacataaacgcattactgaggtgcttcggattagttttgatggactggatgaaaaagaccaggaaatattcctacatatcgcttgcttttacaaggggaaggataaggatcgtgtgacacaaatactagactattgccagctaaaccctgtcattggtttaagtgttcttgctgataaatctctcataactatctccaacaacaaactgtcgatgcatgatttgctacaagaaatgggctgggaaattgttcgtgaacagtctcctaaagagccaggcaaacgtagtagattatggtctcatgaagacatcaacaatgtgctgaagagacataagggaacagattcaatccaaggcatggtaatggagttgactaaattacaagtggctcattggaatccagaagcctttacaaatttgtctgaacttggtcttctccatattcgtaatgtggaccttcccaaaggcctcacttgtctttctagttccttgagactccttgaatggactgggtatcccttaagatctctcccaaaattttttgaagcagatgaacttattgaacttaacttgtgccacagcaacattaaacagctttggaagggaacaaaggctattcttctccatattcgtaatgtggaccttcctgaaggcctcacttgtctttctaattccttgagactcctggaatggactgggtatcccttaagatctctcccacaaaactttgaagcagatgatctcattgaacttaacctgtgccacagcaacattgcatatctttggaagggaacaaagaattttgacaggttgaagttcatcaaactctgccactctcaaaacattttggagaccccagacctcacaggcgttcagaatcttgagtgtttagatcttgaaggatgtgagaatttggtaagagtccatcaatcccttggatttctcaaaaagcttaatgacctgaatcttaaagactgcaaaagtctcgagagtctgccaagtataattgaaatggaatctctcgaaacattaattctttctaactgcttaaaagttaagaagattcccgagtttgttggaaatatggagcgtttgtcggtgctttatcttgatgagactgccattgaggaactgcctgtgtcaattgaacggctgagtggccttgtgtcattgaatctgagcaactgcaaaaatcttgtctgtcttccgagcaccatcaataaattgaagtctattagaaatcttaatctttctggatgcttgaaacttggcaaacatcaggtaaatgtgggggaaaatgatgtgaatagtgggtctgcaatagaattgtcatctacccatgatctcataaaatatgtgagaggttcagtttttcgtggatgcgaagtagtttggggatctgtaaataagttcttgccttctggattggtgcaaaaagtgaatacagagccaatgagtttccgcttgcctatatctggtctgtgtaatttaacatatctgaacctgagtaattgcaatcttggtgaaggagcatttgcaaacgaatttggttactttccctctttggtgaccttggatctaagtggaaacaattttgttcgtcttccttcaggcattggattgctttctaagcttgagaacttgaacttggagaattgcaagagacttcaagagctgtcaaaccttccatcaaatagtatactagatttaagggcagatggtttttttgcactccatgagcaccatcgggtggatgagctttatattgataaattcaagacttttaatgcaacacatcatcttgtatgttgcctaaagctcgatggaagagaattggaagtatatggcatacagcctgcatttcgatttagtgaagagttttgccaggttgagtcagatcacctgtggctattctatgtatctcatgataaattctttggtgcagagtggtggcataacagttgcagtcagcttgagttcttttttgaaactagagggctgggtctgaaggtgaaggagtgtggagtccatctgatatatgagcaagatgtgcaagagttgaaccaaacaaccactcaatcaagcagtaggatgcctcctaatgaggatatattgattggttttgactttctggagctctcttgccttcatgttttgccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1141

Amino Acids

129.71

Weight (kDa)

6.75

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 755
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1621, 3013
AccI GTMKAC 1 cut(s) 2962
AccII CGCG 1 cut(s) 206
AciI CCGC 9 cut(s) 70, 204, 340, 604, 638, 648, 679, 886, 2725
AclWI GGATC 9 cut(s) 195, 320, 333, 661, 674, 936, 1380, 2668, 2847
AcuI CTGAAG 4 cut(s) 1607, 1932, 2856, 3290
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 5 cut(s) 649, 1418, 1621, 2738, 3013
AflII CTTAAG 2 cut(s) 1783, 1969
AjnI CCWGG 6 cut(s) 443, 979, 1323, 1537, 1947, 3147
AjuI GAANNNNNNNTTGG 2 cut(s) 2894, 2926
Alw21I GWGCWC 4 cut(s) 366, 2477, 3006, 3402
Alw26I GTCTC 9 cut(s) 1530, 1566, 1585, 1750, 1936, 2108, 2252, 2372, 2920
AlwI GGATC 9 cut(s) 195, 320, 333, 661, 674, 936, 1380, 2668, 2847
Ama87I CYCGRG 4 cut(s) 705, 1167, 2249, 2330
AoxI GGCC 6 cut(s) 167, 292, 787, 1729, 1915, 2422
ApeKI GCWGC 4 cut(s) 182, 452, 869, 966
ArsI GACNNNNNNTTYG 2 cut(s) 2445, 2477
AseI ATTAAT 1 cut(s) 2294
Asp700I GAANNNNTTC 5 cut(s) 598, 810, 1080, 1512, 1606
AspLEI GCGC 1 cut(s) 966
AspS9I GGNCC 4 cut(s) 292, 977, 1717, 1903
AsuHPI GGTGA 5 cut(s) 115, 2798, 2842, 3155, 3286
AvaI CYCGRG 4 cut(s) 705, 1167, 2249, 2330
AvaII GGWCC 3 cut(s) 977, 1717, 1903
BamHI GGATCC 2 cut(s) 325, 666
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 2 cut(s) 366, 3402
BbsI GAAGAC 6 cut(s) 304, 864, 1575, 1688, 2459, 2858
Bbv12I GWGCWC 4 cut(s) 366, 2477, 3006, 3402
BbvI GCAGC 4 cut(s) 169, 439, 856, 978
BccI CCATC 9 cut(s) 283, 1298, 2196, 2486, 2959, 2975, 3015, 3080, 3300
BceAI ACGGC 1 cut(s) 2429
BcgI CGANNNNNNTGC 2 cut(s) 2230, 2264
BciT130I CCWGG 6 cut(s) 445, 981, 1325, 1539, 1949, 3149
BciVI GTATCC 2 cut(s) 1788, 1974
BcoDI GTCTC 9 cut(s) 1530, 1566, 1585, 1750, 1936, 2108, 2252, 2372, 2920
BfaI CTAG 7 cut(s) 198, 791, 1187, 1391, 1746, 2966, 3255
BfrI CTTAAG 2 cut(s) 1783, 1969
BfuAI ACCTGC 1 cut(s) 755
BfuI GTATCC 2 cut(s) 1788, 1974
BglII AGATCT 3 cut(s) 1787, 1973, 2155
BisI GCNGC 5 cut(s) 183, 341, 453, 870, 967
BlsI GCNGC 5 cut(s) 184, 342, 454, 871, 968
Bme1390I CCNGG 6 cut(s) 445, 981, 1325, 1539, 1949, 3149
Bme18I GGWCC 3 cut(s) 977, 1717, 1903
BmeT110I CYCGRG 4 cut(s) 705, 1167, 2249, 2330
BmgT120I GGNCC 4 cut(s) 292, 977, 1717, 1903
BmiI GGNNCC 5 cut(s) 327, 344, 378, 601, 668
BmrFI CCNGG 6 cut(s) 445, 981, 1325, 1539, 1949, 3149
BmrI ACTGGG 2 cut(s) 1782, 1968
BmsI GCATC 6 cut(s) 127, 1030, 1467, 2510, 2633, 3345
BmuI ACTGGG 2 cut(s) 1782, 1968
BoxI GACNNNNGTC 1 cut(s) 464
BpiI GAAGAC 6 cut(s) 304, 864, 1575, 1688, 2459, 2858
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 3 cut(s) 466, 978, 3416
BpuEI CTTGAG 6 cut(s) 167, 1774, 1960, 2165, 2918, 3257
BsaAI YACGTR 1 cut(s) 631
BsaBI GATNNNNATC 2 cut(s) 927, 1371
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1566, 2108
BsaJI CCNNGG 4 cut(s) 980, 1615, 2197, 2835
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 2342, 2372
Bsc4I CCNNNNNNNGG 5 cut(s) 649, 1418, 1621, 2738, 3013
Bse1I ACTGG 5 cut(s) 88, 1124, 1314, 1777, 1963
Bse3DI GCAATG 1 cut(s) 2034
Bse8I GATNNNNATC 2 cut(s) 927, 1371
BseBI CCWGG 6 cut(s) 445, 981, 1325, 1539, 1949, 3149
BseDI CCNNGG 4 cut(s) 980, 1615, 2197, 2835
BseGI GGATG 8 cut(s) 139, 259, 1318, 2171, 2525, 2648, 3022, 3360
BseJI GATNNNNATC 2 cut(s) 927, 1371
BseLI CCNNNNNNNGG 5 cut(s) 649, 1418, 1621, 2738, 3013
BseMI GCAATG 1 cut(s) 2034
BseMII CTCAG 6 cut(s) 453, 513, 1272, 2408, 2432, 2759
BseNI ACTGG 5 cut(s) 88, 1124, 1314, 1777, 1963
BseXI GCAGC 4 cut(s) 169, 439, 856, 978
BseYI CCCAGC 2 cut(s) 1503, 3262
BsgI GTGCAG 2 cut(s) 84, 3225
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 6 cut(s) 169, 294, 789, 1731, 1917, 2424
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 4 cut(s) 366, 2477, 3006, 3402
BsiHKCI CYCGRG 4 cut(s) 705, 1167, 2249, 2330
BslFI GGGAC 1 cut(s) 1101
BslI CCNNNNNNNGG 5 cut(s) 649, 1418, 1621, 2738, 3013
BsmAI GTCTC 9 cut(s) 1530, 1566, 1585, 1750, 1936, 2108, 2252, 2372, 2920
BsmFI GGGAC 1 cut(s) 1101
BsmI GAATGC 2 cut(s) 502, 606
BsnI GGCC 6 cut(s) 169, 294, 789, 1731, 1917, 2424
Bso31I GGTCTC 2 cut(s) 1566, 2108
BsoBI CYCGRG 4 cut(s) 705, 1167, 2249, 2330
Bsp1286I GDGCHC 4 cut(s) 366, 2477, 3006, 3402
BspACI CCGC 9 cut(s) 70, 204, 340, 604, 638, 648, 679, 886, 2725
BspANI GGCC 6 cut(s) 169, 294, 789, 1731, 1917, 2424
BspCNI CTCAG 6 cut(s) 454, 514, 1273, 2409, 2433, 2760
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 3 cut(s) 610, 1564, 3187
BspLI GGNNCC 5 cut(s) 327, 344, 378, 601, 668
BspMI ACCTGC 1 cut(s) 755
BspPI GGATC 9 cut(s) 195, 320, 333, 661, 674, 936, 1380, 2668, 2847
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 2 cut(s) 1783, 1969
BspTNI GGTCTC 2 cut(s) 1566, 2108
BsrDI GCAATG 1 cut(s) 2034
BsrI ACTGG 5 cut(s) 88, 1124, 1314, 1777, 1963
BssECI CCNNGG 4 cut(s) 980, 1615, 2197, 2835
BssNAI GTATAC 1 cut(s) 2963
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 1615, 2197, 2835
Bst1107I GTATAC 1 cut(s) 2963
Bst2UI CCWGG 6 cut(s) 445, 981, 1325, 1539, 1949, 3149
Bst4CI ACNGT 5 cut(s) 1070, 1225, 1473, 1524, 3224
Bst6I CTCTTC 4 cut(s) 19, 1583, 3085, 3132
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 2 cut(s) 1783, 1969
BstAPI GCANNNNNTGC 2 cut(s) 1486, 3313
BstBAI YACGTR 1 cut(s) 631
BstC8I GCNNGC 8 cut(s) 485, 638, 677, 831, 1348, 1403, 2729, 3120
BstDEI CTNAG 9 cut(s) 462, 522, 777, 1281, 2417, 2441, 2768, 2843, 2892
BstEII GGTNACC 1 cut(s) 2830
BstF5I GGATG 8 cut(s) 139, 259, 1318, 2171, 2525, 2648, 3022, 3360
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 1 cut(s) 966
BstMAI GTCTC 9 cut(s) 1530, 1566, 1585, 1750, 1936, 2108, 2252, 2372, 2920
BstMWI GCNNNNNNNGC 6 cut(s) 349, 449, 681, 1486, 2893, 3313
BstNI CCWGG 6 cut(s) 445, 981, 1325, 1539, 1949, 3149
BstNSI RCATGY 1 cut(s) 679
BstPAI GACNNNNGTC 1 cut(s) 464
BstPI GGTNACC 1 cut(s) 2830
BstSCI CCNGG 6 cut(s) 443, 979, 1323, 1537, 1947, 3147
BstSNI TACGTA 1 cut(s) 631
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 4 cut(s) 169, 439, 856, 978
BstV2I GAAGAC 6 cut(s) 304, 864, 1575, 1688, 2459, 2858
BstX2I RGATCY 8 cut(s) 200, 325, 666, 1787, 1973, 2155, 2660, 2839
BstYI RGATCY 8 cut(s) 200, 325, 666, 1787, 1973, 2155, 2660, 2839
BstZ17I GTATAC 1 cut(s) 2963
BsuI GTATCC 2 cut(s) 1788, 1974
BsuRI GGCC 6 cut(s) 169, 294, 789, 1731, 1917, 2424
BtsCI GGATG 8 cut(s) 139, 259, 1318, 2171, 2525, 2648, 3022, 3360
BtsIMutI CAGTG 1 cut(s) 1221
BveI ACCTGC 1 cut(s) 755
Cac8I GCNNGC 8 cut(s) 485, 638, 677, 831, 1348, 1403, 2729, 3120
CciI TCATGA 3 cut(s) 610, 1564, 3187
CfoI GCGC 1 cut(s) 966
Cfr13I GGNCC 4 cut(s) 292, 977, 1717, 1903
DdeI CTNAG 9 cut(s) 462, 522, 777, 1281, 2417, 2441, 2768, 2843, 2892
DraI TTTAAA 1 cut(s) 841
Eam1104I CTCTTC 4 cut(s) 19, 1583, 3085, 3132
EarI CTCTTC 4 cut(s) 19, 1583, 3085, 3132
Ecl136II GAGCTC 2 cut(s) 364, 3400
Eco105I TACGTA 1 cut(s) 631
Eco130I CCWWGG 3 cut(s) 1615, 2197, 2835
Eco147I AGGCCT 3 cut(s) 169, 1731, 1917
Eco24I GRGCYC 2 cut(s) 366, 3402
Eco31I GGTCTC 2 cut(s) 1566, 2108
Eco47I GGWCC 3 cut(s) 977, 1717, 1903
Eco53kI GAGCTC 2 cut(s) 364, 3400
Eco57I CTGAAG 4 cut(s) 1607, 1932, 2856, 3290
Eco88I CYCGRG 4 cut(s) 705, 1167, 2249, 2330
Eco91I GGTNACC 1 cut(s) 2830
EcoICRI GAGCTC 2 cut(s) 364, 3400
EcoO65I GGTNACC 1 cut(s) 2830
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 6 cut(s) 443, 979, 1323, 1537, 1947, 3147
EcoT14I CCWWGG 3 cut(s) 1615, 2197, 2835
EcoT22I ATGCAT 2 cut(s) 281, 1482
EcoT38I GRGCYC 2 cut(s) 366, 3402
ErhI CCWWGG 3 cut(s) 1615, 2197, 2835
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 1 cut(s) 1101
FauI CCCGC 5 cut(s) 77, 611, 641, 645, 672
FblI GTMKAC 1 cut(s) 2962
Fnu4HI GCNGC 5 cut(s) 183, 341, 453, 870, 967
FokI GGATG 8 cut(s) 146, 266, 1325, 2178, 2532, 2655, 3029, 3367
FriOI GRGCYC 2 cut(s) 366, 3402
Fsp4HI GCNGC 5 cut(s) 183, 341, 453, 870, 967
FspBI CTAG 7 cut(s) 198, 791, 1187, 1391, 1746, 2966, 3255
GlaI GCGC 1 cut(s) 965
GluI GCNGC 5 cut(s) 183, 341, 453, 870, 967
GsaI CCCAGC 2 cut(s) 1507, 3266
GsuI CTGGAG 3 cut(s) 466, 978, 3416
HaeIII GGCC 6 cut(s) 169, 294, 789, 1731, 1917, 2424
HhaI GCGC 1 cut(s) 966
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 964
HinP1I GCGC 1 cut(s) 964
HincII GTYRAC 3 cut(s) 9, 577, 1635
HindII GTYRAC 3 cut(s) 9, 577, 1635
HindIII AAGCTT 3 cut(s) 419, 2213, 2894
HphI GGTGA 5 cut(s) 115, 2798, 2842, 3155, 3286
Hpy166II GTNNAC 7 cut(s) 9, 577, 1520, 1635, 1717, 1903, 2963
Hpy8I GTNNAC 7 cut(s) 9, 577, 1520, 1635, 1717, 1903, 2963
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 5 cut(s) 1070, 1225, 1473, 1524, 3224
HpyCH4IV ACGT 2 cut(s) 630, 1546
HpyF10VI GCNNNNNNNGC 6 cut(s) 349, 449, 681, 1486, 2893, 3313
HpyF3I CTNAG 9 cut(s) 462, 522, 777, 1281, 2417, 2441, 2768, 2843, 2892
HpySE526I ACGT 2 cut(s) 630, 1546
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 964
LmnI GCTCC 4 cut(s) 866, 2350, 2792, 3397
Lsp1109I GCAGC 4 cut(s) 169, 439, 856, 978
LweI GCATC 6 cut(s) 127, 1030, 1467, 2510, 2633, 3345
MaeI CTAG 7 cut(s) 198, 791, 1187, 1391, 1746, 2966, 3255
MaeII ACGT 2 cut(s) 630, 1546
MaeIII GTNAC 3 cut(s) 1378, 2813, 2830
MfeI CAATTG 2 cut(s) 186, 2406
MflI RGATCY 8 cut(s) 200, 325, 666, 1787, 1973, 2155, 2660, 2839
MhlI GDGCHC 4 cut(s) 366, 2477, 3006, 3402
MlyI GAGTC 7 cut(s) 188, 1752, 1938, 2193, 2263, 3164, 3297
MmeI TCCRAC 2 cut(s) 1485, 2320
Mph1103I ATGCAT 2 cut(s) 281, 1482
MroXI GAANNNNTTC 5 cut(s) 598, 810, 1080, 1512, 1606
MslI CAYNNNNRTG 3 cut(s) 128, 593, 1625
MspA1I CMGCKG 1 cut(s) 969
MspCI CTTAAG 2 cut(s) 1783, 1969
MspR9I CCNGG 6 cut(s) 445, 981, 1325, 1539, 1949, 3149
MunI CAATTG 2 cut(s) 186, 2406
Mva1269I GAATGC 2 cut(s) 502, 606
MvaI CCWGG 6 cut(s) 445, 981, 1325, 1539, 1949, 3149
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 6 cut(s) 349, 449, 681, 1486, 2893, 3313
NlaIV GGNNCC 5 cut(s) 327, 344, 378, 601, 668
NmuCI GTSAC 2 cut(s) 1378, 2830
NsiI ATGCAT 2 cut(s) 281, 1482
NspI RCATGY 1 cut(s) 679
PaeI GCATGC 1 cut(s) 679
PaeR7I CTCGAG 2 cut(s) 705, 2249
PagI TCATGA 3 cut(s) 610, 1564, 3187
PceI AGGCCT 3 cut(s) 169, 1731, 1917
PctI GAATGC 2 cut(s) 502, 606
PdmI GAANNNNTTC 5 cut(s) 598, 810, 1080, 1512, 1606
PfeI GAWTC 9 cut(s) 822, 990, 1607, 1660, 2140, 2227, 2279, 2326, 2437
PflMI CCANNNNNTGG 2 cut(s) 1621, 3013
PfoI TCCNGGA 1 cut(s) 1947
PkrI GCNGC 5 cut(s) 184, 342, 454, 871, 968
PleI GAGTC 7 cut(s) 188, 1752, 1938, 2192, 2262, 3163, 3296
PpsI GAGTC 7 cut(s) 188, 1752, 1938, 2192, 2262, 3163, 3296
Ppu21I YACGTR 1 cut(s) 631
PshAI GACNNNNGTC 1 cut(s) 464
PshBI ATTAAT 1 cut(s) 2294
Psp124BI GAGCTC 2 cut(s) 366, 3402
Psp6I CCWGG 6 cut(s) 443, 979, 1323, 1537, 1947, 3147
PspEI GGTNACC 1 cut(s) 2830
PspFI CCCAGC 2 cut(s) 1503, 3262
PspGI CCWGG 6 cut(s) 443, 979, 1323, 1537, 1947, 3147
PspN4I GGNNCC 5 cut(s) 327, 344, 378, 601, 668
PspPI GGNCC 4 cut(s) 292, 977, 1717, 1903
PspXI VCTCGAGB 1 cut(s) 705
PsuI RGATCY 8 cut(s) 200, 325, 666, 1787, 1973, 2155, 2660, 2839
PvuII CAGCTG 1 cut(s) 969
RseI CAYNNNNRTG 3 cut(s) 128, 593, 1625
SacI GAGCTC 2 cut(s) 366, 3402
SatI GCNGC 5 cut(s) 183, 341, 453, 870, 967
Sau96I GGNCC 4 cut(s) 292, 977, 1717, 1903
SchI GAGTC 7 cut(s) 188, 1752, 1938, 2193, 2263, 3164, 3297
ScrFI CCNGG 6 cut(s) 445, 981, 1325, 1539, 1949, 3149
SduI GDGCHC 4 cut(s) 366, 2477, 3006, 3402
SfaNI GCATC 6 cut(s) 127, 1030, 1467, 2510, 2633, 3345
Sfr274I CTCGAG 2 cut(s) 705, 2249
SinI GGWCC 3 cut(s) 977, 1717, 1903
SlaI CTCGAG 2 cut(s) 705, 2249
SmiMI CAYNNNNRTG 3 cut(s) 128, 593, 1625
SnaBI TACGTA 1 cut(s) 631
SphI GCATGC 1 cut(s) 679
SseBI AGGCCT 3 cut(s) 169, 1731, 1917
SsiI CCGC 9 cut(s) 70, 204, 340, 604, 638, 648, 679, 886, 2725
SspI AATATT 1 cut(s) 1332
SspMI CTAG 7 cut(s) 198, 791, 1187, 1391, 1746, 2966, 3255
SstI GAGCTC 2 cut(s) 366, 3402
StuI AGGCCT 3 cut(s) 169, 1731, 1917
StyD4I CCNGG 6 cut(s) 443, 979, 1323, 1537, 1947, 3147
StyI CCWWGG 3 cut(s) 1615, 2197, 2835
TaaI ACNGT 5 cut(s) 1070, 1225, 1473, 1524, 3224
TaiI ACGT 2 cut(s) 633, 1549
TaqI TCGA 6 cut(s) 706, 1475, 2250, 2286, 3084, 3127
TaqII GACCGA 1 cut(s) 756
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 9 cut(s) 822, 990, 1607, 1660, 2140, 2227, 2279, 2326, 2437
TscAI CASTG 1 cut(s) 1228
TseFI GTSAC 2 cut(s) 1378, 2830
TseI GCWGC 4 cut(s) 182, 452, 869, 966
Tsp45I GTSAC 2 cut(s) 1378, 2830
TspGWI ACGGA 3 cut(s) 544, 716, 1167
TspRI CASTG 1 cut(s) 1228
Van91I CCANNNNNTGG 2 cut(s) 1621, 3013
Vha464I CTTAAG 2 cut(s) 1783, 1969
VpaK11BI GGWCC 3 cut(s) 977, 1717, 1903
VspI ATTAAT 1 cut(s) 2294
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 679
XcmI CCANNNNNNNNNTGG 2 cut(s) 826, 2195
XhoI CTCGAG 2 cut(s) 705, 2249
XmiI GTMKAC 1 cut(s) 2962
XmnI GAANNNNTTC 5 cut(s) 598, 810, 1080, 1512, 1606
XspI CTAG 7 cut(s) 198, 791, 1187, 1391, 1746, 2966, 3255
Zsp2I ATGCAT 2 cut(s) 281, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.