RchiOBHm_Chr6g0250551

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000105
Physical Location & Seq
Forward (+)
6020375 .. 6026891
6517 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22460

Sequence Viewer

Length: 3558 bp
ATGGCGTCAACTTGGTCTCCTCGTCGACTATACAAATACGATGTGTTTCTAAGCTTCAGAGGGGAAGATACCCGCGAGACTTTCATCTGCCATCTGTATCGTGCGCTAACTGCGAAAGGAATTATAACCTTCATAGATGACGAAAGCCTGGAGAGAGGATCAACCATTGGCCCATCACTTTTTACAGCAATTCAGGACTCAAGATTCGCCCTTGTCGTTTTTTCAGAGAATTTCGCTTCTTCATCCTGGTGCTTGGATGAACTCTCCAAGATTTTTCAATGCTTGGTAAGGAGGGGACGATTCGTCTTCCCGATCTTCTACCGAGTGGCTCCCTCTGATGTGCGCAAGCAAACGGGAAGTTTTCAGCTGCGTACAGTAGCGGTAAGGGAACATGAAGAAGTTTATGGAAATAAGGACAAACTAGAGGAGTGGAGACATGCTTTGACAACGGTTGGCGGTATTGGTGGGTGGGTCTCGGAGGATTATAAGGATGACGGAAAATTGATTGAGGACGTTGTTACGTATACACTTCGGAGACTAGGTCATACTTATACCTCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTATTAAGCAATTACATATGTCCGCAGCTTGGTGGGGTGCGTTTTATAGGCATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCCATCCATGATCGAATTTGTCAGGATTTTGACCAAAGCTGCATTCTTTCCAATGTTAGAGAAATATCTGAAAAGGATGGCCTAGTTTCTCTTCAAGAAAAACTACTTTCCAGAATCCTGAGGACAAAAGTTGACATTGAGGATGAATATGCAGGAGCTACTATGATAGAAAGGCGGTTATGTAAGCTAAAGGTGCTTGTTGTTATTGATGATGTGAATCACATCAATCAATTAGATAAATTGGCTGGAAGTCGGGACTGGTTCGGTCCTGGGAGTAGAATTATTGTAACCACCCCAGATATTCATTTGCTAAGGGGACATGATGTTGATGCTACATACAAGCCTACTGGGTTAAGTGATGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAAAAGCTTCCCACCAGAAGATTATTTGGACTTGTGCCACCATATTATAGGGTATGCTCAGGGGCTTCCATTGTCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGACCCAATGAATGGGAAAGTGCAATAGAGAGGCTAAACAATACACCAAATAGACAAGTTATGGATGTGCTTCAGATTAGTTTTGATGGACTACAGGAAAAAGACCAACAGATATTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGGCTATTGCAAGCTAGAACCTGGCATTGGTTTAAGGGTTCTTGAGGAAAAATCTCTCATTACAATTTTTAACAACAAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTTGGAAATAGTTCGTCGAGAGTCCCCTGATGAGCCAGGTAAACGAAGTAGATTATGGTCTCCTGAAGATATCCACAAAGTGCTGAAGAAAAATAAGGGAACAGACAAAATCCGAGCCATGGTAATGGACTTGCCTGAGTTACAAGTGGCTCATTGGAAACCAGAAGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCGTAACGTGGATCTTCCCAAAGGCCTCACTTTTTTATCTAATTCATTGAGGCTCCTTGATTGGAGTGGGTACCCCTTAAGATCCCTCCCACAAAATTTTGAACCAGACGAACTTATTGAACTTAACTTGTGCCACAGCAACATTGAACACCTCTGGGAAGGAGCAAAGAATTTTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCAGACCCCAGACCTCACAGGCGTTCAAAATCTTGAGACTATAGATCTTGAAGGATGTAAGACTTTGGTAAAACTTCATCAATCTGTCGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAAGATTGCGAGAGTCTCGAGAATATTCCAAGTAAGATTGAAATGGAATCTCTTGAAACTTTAATTCTTTCTAACTGCTCAAAAGTTAAAAAGATTCCCGAGTTCGCTGGAAATATGAAGCGTTTATCAAAGCTTTATCTCAATGAGACTGCCATTGAAAAACTACCTGTGTCAATTGGATGCCTGAGTGGCCTTGCTTCACTGAATCTTAGCAACTGCAAAAATCTTCTATGCCTTGCAAGCACCTTAGAACTGCTTAGTAGATTGGAGTCTCTTAAAAAGCTTGATCTTTCTGGATGTTTGAAACTTCGAGAACAGGATAGTGTGAGGGAGAATGGTGCAATTAGAACTGCTGTAAATCATCTTATAAAACGTGGTTGTGAAGTTGGGAAATATTTGTCATGGTCCTTGCCTTCTAGATTGGTGCAAAGAGCCTATATGGAGCCAATGAGTATGAAGTTGCCTGTGTCTGATCTGTGTTATTCTACAGAGCAAAATGCAAATACATCAATGAGTATCCGGTTGCCACTATCTCGTCTATGGAATTTAACAGATCTAAATTTAAGTAACTGCAATCTTGGTGACACTGCATTTGCCAACTTTGCCTGTTTTCCCTCTTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTAAGCTTCAGAACATTAATTTGGAGAATTGCAAGACACTTGAAGAGTTGTCAGGACTTCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAAATGCTGTTTGATGTATCCAATTTCAATAGATTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTGCTGTAACATAGCATTTGAAATGCTGAGGATATTCCTTAATCAGCAAGTTTCTAGTGTGACAGAAACTTTTCAAATTGTAATTCCTGGAAGTGGAATTCCTGAGTGGTTTGATCATCGAAGATTTGGGAGTTCATTAACTGTAGACCTACTTCCAGATTGGAATGAGAGTAGGTTTTTGGGATTTGTTTTGTGTGCTGTTTTTGTACTCCATGAGCACCGTCAGGTTGATGAGCTTGATATCCATCAGTTCAAGACTTTTAAGGCCACTCATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTAGAAGTATACGGCAAACAGCCTGCATTTCGCTTTAGCGAACAATTTTGCCAGGTTGAGTCAGATCACCTTTGGATATTCTATGTGTCCCGTGATAAATACTTTGGTACAGATTGGTGGCATAACAGTTGCAGTCAGCTTGAGTTCTTATTTGAAACAAGAGGGCCAGGCCTGAGGGTGAAGGAGTGTGGGGTACGTCTGATATATGAGCAAGATGTGCTAGAGTTGAACCAAACAATAACTCAATCAAGCAGTAGGATGTCTCCTCCATATGAGGATATATTGACTGATTTTGAGAATCAAGTTGAAGGGGAAACCATTCACCCAAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1185

Amino Acids

135.05

Weight (kDa)

6.47

Isoelectric Point (pI)

44.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 193 2.9e-49 TIR domain
TIR_2 PF13676 15 - 111 2.8e-13 TIR domain
NB-ARC PF00931 214 - 368 1.2e-16 NB-ARC domain
WHD_ROQ1 PF23282 435 - 505 1e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 635 - 776 5.7e-08 Leucine-rich repeat region
LRR_13 PF23286 694 - 784 7.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 999 - 1147 4.9e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 125, 486, 2402
Acc16I TGCGCA 1 cut(s) 344
Acc65I GGTACC 1 cut(s) 1764
AccB1I GGYRCC 1 cut(s) 1764
AccB7I CCANNNNNTGG 1 cut(s) 1218
AccI GTMKAC 4 cut(s) 25, 524, 3057, 3231
AccII CGCG 1 cut(s) 75
AciI CCGC 7 cut(s) 73, 380, 456, 595, 629, 670, 874
AclWI GGATC 4 cut(s) 166, 1368, 1713, 1770
AcsI RAATTY 8 cut(s) 229, 714, 1666, 1789, 1864, 2578, 2593, 3009
AcuI CTGAAG 6 cut(s) 40, 1262, 1575, 1595, 2708, 3224
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1570
AfaI GTAC 6 cut(s) 373, 1343, 1766, 3120, 3331, 3417
AfiI CCNNNNNNNGG 5 cut(s) 635, 1218, 1406, 1609, 3005
AflII CTTAAG 1 cut(s) 1771
AhdI GACNNNNNGTC 1 cut(s) 302
AjnI CCWGG 8 cut(s) 147, 245, 967, 1399, 1525, 2998, 3273, 3388
AjuI GAANNNNNNNTTGG 6 cut(s) 726, 758, 1389, 1421, 2720, 2752
Alw21I GWGCWC 1 cut(s) 3132
AlwI GGATC 4 cut(s) 166, 1368, 1713, 1770
AlwNI CAGNNNCTG 1 cut(s) 2712
Ama87I CYCGRG 3 cut(s) 696, 2051, 2132
AoxI GGCC 7 cut(s) 169, 778, 1717, 2224, 3177, 3386, 3391
ApeKI GCWGC 4 cut(s) 367, 631, 738, 2913
ApoI RAATTY 8 cut(s) 229, 714, 1666, 1789, 1864, 2578, 2593, 3009
ArsI GACNNNNNNTTYG 4 cut(s) 188, 220, 2609, 2641
AseI ATTAAT 1 cut(s) 2733
Asp700I GAANNNNTTC 6 cut(s) 589, 1068, 1103, 1500, 2982, 3540
Asp718I GGTACC 1 cut(s) 1764
AspLEI GCGC 2 cut(s) 106, 345
AspS9I GGNCC 5 cut(s) 170, 965, 2439, 2709, 3386
AsuHPI GGTGA 5 cut(s) 1073, 2627, 3281, 3412, 3536
AvaI CYCGRG 3 cut(s) 696, 2051, 2132
AvaII GGWCC 3 cut(s) 965, 2439, 2709
AxyI CCTNAGG 2 cut(s) 818, 3395
BanI GGYRCC 1 cut(s) 1764
BarI GAAGNNNNNNTAC 2 cut(s) 3048, 3080
BbsI GAAGAC 3 cut(s) 298, 1213, 1676
Bbv12I GWGCWC 1 cut(s) 3132
BbvCI CCTCAGC 1 cut(s) 2939
BbvI GCAGC 4 cut(s) 354, 643, 725, 2900
BccI CCATC 9 cut(s) 99, 181, 710, 770, 1052, 1286, 2785, 2801, 3165
BceAI ACGGC 1 cut(s) 3250
BciT130I CCWGG 8 cut(s) 149, 247, 969, 1401, 1527, 3000, 3275, 3390
BciVI GTATCC 2 cut(s) 2561, 2851
BclI TGATCA 1 cut(s) 3025
BfaI CTAG 8 cut(s) 422, 539, 782, 1379, 1394, 2451, 2967, 3443
BfmI CTRYAG 4 cut(s) 1298, 1953, 2520, 3054
BfrI CTTAAG 1 cut(s) 1771
BfuI GTATCC 2 cut(s) 2561, 2851
BglI GCCNNNNNGGC 1 cut(s) 2223
BglII AGATCT 2 cut(s) 1957, 2587
BisI GCNGC 4 cut(s) 368, 632, 739, 2914
BlsI GCNGC 4 cut(s) 369, 633, 740, 2915
Bme1390I CCNGG 8 cut(s) 149, 247, 969, 1401, 1527, 3000, 3275, 3390
Bme18I GGWCC 3 cut(s) 965, 2439, 2709
BmeRI GACNNNNNGTC 1 cut(s) 302
BmeT110I CYCGRG 3 cut(s) 696, 2051, 2132
BmgT120I GGNCC 5 cut(s) 170, 965, 2439, 2709, 3386
BmiI GGNNCC 4 cut(s) 330, 1748, 1766, 2478
BmrFI CCNGG 8 cut(s) 149, 247, 969, 1401, 1527, 3000, 3275, 3390
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 5 cut(s) 666, 1018, 1455, 2204, 2712
BmuI ACTGGG 1 cut(s) 1056
BpiI GAAGAC 3 cut(s) 298, 1213, 1676
BpmI CTGGAG 1 cut(s) 170
Bpu10I CCTNAGC 3 cut(s) 1010, 1155, 2939
BpuEI CTTGAG 4 cut(s) 184, 1442, 1967, 3383
BsaAI YACGTR 1 cut(s) 522
BsaBI GATNNNNATC 2 cut(s) 915, 3156
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 3 cut(s) 21, 478, 1554
BsaJI CCNNGG 3 cut(s) 661, 968, 1608
BsaWI WCCGGW 1 cut(s) 2553
BsaXI ACNNNNNCTCC 3 cut(s) 31, 526, 556
Bsc4I CCNNNNNNNGG 5 cut(s) 635, 1218, 1406, 1609, 3005
Bse1I ACTGG 3 cut(s) 962, 1051, 2826
Bse21I CCTNAGG 2 cut(s) 818, 3395
Bse8I GATNNNNATC 2 cut(s) 915, 3156
BseBI CCWGG 8 cut(s) 149, 247, 969, 1401, 1527, 3000, 3275, 3390
BseDI CCNNGG 3 cut(s) 661, 968, 1608
BseJI GATNNNNATC 2 cut(s) 915, 3156
BseLI CCNNNNNNNGG 5 cut(s) 635, 1218, 1406, 1609, 3005
BseMII CTCAG 9 cut(s) 809, 1169, 1617, 1693, 2210, 2660, 2930, 3006, 3386
BseNI ACTGG 3 cut(s) 962, 1051, 2826
BseRI GAGGAG 3 cut(s) 9, 440, 3477
BseXI GCAGC 4 cut(s) 354, 643, 725, 2900
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 7 cut(s) 171, 780, 1719, 2226, 3179, 3388, 3393
BshNI GGYRCC 1 cut(s) 1764
BsiHKAI GWGCWC 1 cut(s) 3132
BsiHKCI CYCGRG 3 cut(s) 696, 2051, 2132
BsiSI CCGG 1 cut(s) 2554
BslFI GGGAC 5 cut(s) 309, 968, 1029, 1498, 3295
BslI CCNNNNNNNGG 5 cut(s) 635, 1218, 1406, 1609, 3005
BsmFI GGGAC 5 cut(s) 309, 968, 1029, 1498, 3295
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 7 cut(s) 171, 780, 1719, 2226, 3179, 3388, 3393
Bso31I GGTCTC 3 cut(s) 21, 478, 1554
BsoBI CYCGRG 3 cut(s) 696, 2051, 2132
Bsp1286I GDGCHC 1 cut(s) 3132
Bsp1407I TGTACA 1 cut(s) 1341
Bsp19I CCATGG 2 cut(s) 661, 1608
BspACI CCGC 7 cut(s) 73, 380, 456, 595, 629, 670, 874
BspANI GGCC 7 cut(s) 171, 780, 1719, 2226, 3179, 3388, 3393
BspCNI CTCAG 9 cut(s) 810, 1168, 1618, 1692, 2211, 2661, 2931, 3007, 3387
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 4 cut(s) 330, 1748, 1766, 2478
BspPI GGATC 4 cut(s) 166, 1368, 1713, 1770
BspT107I GGYRCC 1 cut(s) 1764
BspTI CTTAAG 1 cut(s) 1771
BspTNI GGTCTC 3 cut(s) 21, 478, 1554
BsrGI TGTACA 1 cut(s) 1341
BsrI ACTGG 3 cut(s) 962, 1051, 2826
BssECI CCNNGG 3 cut(s) 661, 968, 1608
BssNAI GTATAC 2 cut(s) 525, 3232
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 661, 1608
Bst1107I GTATAC 2 cut(s) 525, 3232
Bst2UI CCWGG 8 cut(s) 149, 247, 969, 1401, 1527, 3000, 3275, 3390
Bst4CI ACNGT 6 cut(s) 376, 451, 1461, 3055, 3134, 3350
Bst6I CTCTTC 4 cut(s) 562, 795, 2754, 3211
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1771
BstAPI GCANNNNNTGC 2 cut(s) 1474, 3439
BstAUI TGTACA 1 cut(s) 1341
BstBAI YACGTR 1 cut(s) 522
BstC8I GCNNGC 5 cut(s) 347, 668, 1391, 2275, 3246
BstDSI CCRYGG 2 cut(s) 661, 1608
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 2 cut(s) 106, 345
BstMWI GCNNNNNNNGC 9 cut(s) 110, 663, 672, 892, 1474, 2223, 2274, 2636, 3439
BstNI CCWGG 8 cut(s) 149, 247, 969, 1401, 1527, 3000, 3275, 3390
BstNSI RCATGY 2 cut(s) 440, 670
BstSCI CCNGG 8 cut(s) 147, 245, 967, 1399, 1525, 2998, 3273, 3388
BstSFI CTRYAG 4 cut(s) 1298, 1953, 2520, 3054
BstSNI TACGTA 1 cut(s) 522
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 4 cut(s) 354, 643, 725, 2900
BstV2I GAAGAC 3 cut(s) 298, 1213, 1676
BstX2I RGATCY 4 cut(s) 1705, 1775, 1957, 2587
BstXI CCANNNNNNTGG 1 cut(s) 1615
BstYI RGATCY 4 cut(s) 1705, 1775, 1957, 2587
BstZ17I GTATAC 2 cut(s) 525, 3232
Bsu36I CCTNAGG 2 cut(s) 818, 3395
BsuI GTATCC 2 cut(s) 2561, 2851
BsuRI GGCC 7 cut(s) 171, 780, 1719, 2226, 3179, 3388, 3393
BtgI CCRYGG 2 cut(s) 661, 1608
BtsI GCAGTG 1 cut(s) 2619
BtsIMutI CAGTG 3 cut(s) 2234, 2619, 2819
Cac8I GCNNGC 5 cut(s) 347, 668, 1391, 2275, 3246
CaiI CAGNNNCTG 1 cut(s) 2712
CfoI GCGC 2 cut(s) 106, 345
Cfr13I GGNCC 5 cut(s) 170, 965, 2439, 2709, 3386
Csp6I GTAC 6 cut(s) 372, 1342, 1765, 3119, 3330, 3416
CviQI GTAC 6 cut(s) 372, 1342, 1765, 3119, 3330, 3416
DraI TTTAAA 1 cut(s) 1086
DraIII CACNNNGTG 1 cut(s) 1570
DriI GACNNNNNGTC 1 cut(s) 302
Eam1104I CTCTTC 4 cut(s) 562, 795, 2754, 3211
Eam1105I GACNNNNNGTC 1 cut(s) 302
EarI CTCTTC 4 cut(s) 562, 795, 2754, 3211
Eco105I TACGTA 1 cut(s) 522
Eco130I CCWWGG 2 cut(s) 661, 1608
Eco147I AGGCCT 2 cut(s) 1719, 3393
Eco31I GGTCTC 3 cut(s) 21, 478, 1554
Eco32I GATATC 2 cut(s) 1561, 3154
Eco47I GGWCC 3 cut(s) 965, 2439, 2709
Eco57I CTGAAG 6 cut(s) 40, 1262, 1575, 1595, 2708, 3224
Eco81I CCTNAGG 2 cut(s) 818, 3395
Eco88I CYCGRG 3 cut(s) 696, 2051, 2132
EcoO109I RGGNCCY 1 cut(s) 2709
EcoRI GAATTC 1 cut(s) 3009
EcoRII CCWGG 8 cut(s) 147, 245, 967, 1399, 1525, 2998, 3273, 3388
EcoRV GATATC 2 cut(s) 1561, 3154
EcoT14I CCWWGG 2 cut(s) 661, 1608
EcoT22I ATGCAT 1 cut(s) 1470
ErhI CCWWGG 2 cut(s) 661, 1608
FalI AAGNNNNNCTT 2 cut(s) 1088, 1120
FaqI GGGAC 5 cut(s) 309, 968, 1029, 1498, 3295
FauI CCCGC 3 cut(s) 80, 602, 663
FauNDI CATATG 2 cut(s) 623, 3493
FbaI TGATCA 1 cut(s) 3025
FblI GTMKAC 4 cut(s) 25, 524, 3057, 3231
Fnu4HI GCNGC 4 cut(s) 368, 632, 739, 2914
Fsp4HI GCNGC 4 cut(s) 368, 632, 739, 2914
FspBI CTAG 8 cut(s) 422, 539, 782, 1379, 1394, 2451, 2967, 3443
FspI TGCGCA 1 cut(s) 344
GlaI GCGC 2 cut(s) 105, 344
GluI GCNGC 4 cut(s) 368, 632, 739, 2914
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 7 cut(s) 171, 780, 1719, 2226, 3179, 3388, 3393
HapII CCGG 1 cut(s) 2554
HhaI GCGC 2 cut(s) 106, 345
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 104, 343
HinP1I GCGC 2 cut(s) 104, 343
HincII GTYRAC 4 cut(s) 9, 26, 568, 832
HindII GTYRAC 4 cut(s) 9, 26, 568, 832
HindIII AAGCTT 6 cut(s) 52, 1102, 2165, 2315, 2690, 2720
HpaII CCGG 1 cut(s) 2554
HphI GGTGA 5 cut(s) 1073, 2627, 3281, 3412, 3536
Hpy166II GTNNAC 8 cut(s) 9, 26, 525, 568, 832, 1532, 3058, 3232
Hpy8I GTNNAC 8 cut(s) 9, 26, 525, 568, 832, 1532, 3058, 3232
Hpy99I CGWCG 2 cut(s) 27, 1509
HpyAV CCTTC 7 cut(s) 139, 1345, 1847, 1958, 2457, 3397, 3524
HpyCH4III ACNGT 6 cut(s) 376, 451, 1461, 3055, 3134, 3350
HpyCH4IV ACGT 5 cut(s) 513, 521, 1701, 2407, 3418
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 663, 672, 892, 1474, 2223, 2274, 2636, 3439
HpySE526I ACGT 5 cut(s) 513, 521, 1701, 2407, 3418
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 104, 343
KpnI GGTACC 1 cut(s) 1768
Ksp22I TGATCA 1 cut(s) 3025
LmnI GCTCC 5 cut(s) 334, 854, 1752, 1856, 2476
Lsp1109I GCAGC 4 cut(s) 354, 643, 725, 2900
LweI GCATC 5 cut(s) 666, 1018, 1455, 2204, 2712
MaeI CTAG 8 cut(s) 422, 539, 782, 1379, 1394, 2451, 2967, 3443
MaeII ACGT 5 cut(s) 513, 521, 1701, 2407, 3418
MaeIII GTNAC 9 cut(s) 517, 985, 1366, 1629, 1697, 2600, 2615, 2918, 2971
MfeI CAATTG 2 cut(s) 2208, 2884
MflI RGATCY 4 cut(s) 1705, 1775, 1957, 2587
MhlI GDGCHC 1 cut(s) 3132
MlyI GAGTC 5 cut(s) 191, 1520, 2056, 2312, 3290
MmeI TCCRAC 1 cut(s) 1981
Mph1103I ATGCAT 1 cut(s) 1470
MroXI GAANNNNTTC 6 cut(s) 589, 1068, 1103, 1500, 2982, 3540
MslI CAYNNNNRTG 3 cut(s) 247, 584, 1613
MspA1I CMGCKG 1 cut(s) 367
MspCI CTTAAG 1 cut(s) 1771
MspI CCGG 1 cut(s) 2554
MspR9I CCNGG 8 cut(s) 149, 247, 969, 1401, 1527, 3000, 3275, 3390
MunI CAATTG 2 cut(s) 2208, 2884
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 8 cut(s) 149, 247, 969, 1401, 1527, 3000, 3275, 3390
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 9 cut(s) 110, 663, 672, 892, 1474, 2223, 2274, 2636, 3439
NcoI CCATGG 2 cut(s) 661, 1608
NdeI CATATG 2 cut(s) 623, 3493
NlaIV GGNNCC 4 cut(s) 330, 1748, 1766, 2478
NmuCI GTSAC 3 cut(s) 1366, 2615, 2971
NsbI TGCGCA 1 cut(s) 344
NsiI ATGCAT 1 cut(s) 1470
NspI RCATGY 2 cut(s) 440, 670
PaeI GCATGC 1 cut(s) 670
PaeR7I CTCGAG 2 cut(s) 696, 2051
PceI AGGCCT 2 cut(s) 1719, 3393
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 6 cut(s) 589, 1068, 1103, 1500, 2982, 3540
PflFI GACNNNGTC 1 cut(s) 540
PflMI CCANNNNNTGG 1 cut(s) 1218
PfoI TCCNGGA 1 cut(s) 2998
PkrI GCNGC 4 cut(s) 369, 633, 740, 2915
PleI GAGTC 5 cut(s) 191, 1519, 2055, 2311, 3289
PpsI GAGTC 5 cut(s) 191, 1519, 2055, 2311, 3289
Ppu21I YACGTR 1 cut(s) 522
PpuMI RGGWCCY 1 cut(s) 2709
PshBI ATTAAT 1 cut(s) 2733
PsiI TTATAA 3 cut(s) 125, 486, 2402
Psp5II RGGWCCY 1 cut(s) 2709
Psp6I CCWGG 8 cut(s) 147, 245, 967, 1399, 1525, 2998, 3273, 3388
PspGI CCWGG 8 cut(s) 147, 245, 967, 1399, 1525, 2998, 3273, 3388
PspN4I GGNNCC 4 cut(s) 330, 1748, 1766, 2478
PspPI GGNCC 5 cut(s) 170, 965, 2439, 2709, 3386
PspPPI RGGWCCY 1 cut(s) 2709
PspXI VCTCGAGB 1 cut(s) 696
PstNI CAGNNNCTG 1 cut(s) 2712
PsuI RGATCY 4 cut(s) 1705, 1775, 1957, 2587
PsyI GACNNNGTC 1 cut(s) 540
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 6 cut(s) 373, 1343, 1766, 3120, 3331, 3417
RsaNI GTAC 6 cut(s) 372, 1342, 1765, 3119, 3330, 3416
RseI CAYNNNNRTG 3 cut(s) 247, 584, 1613
SalI GTCGAC 1 cut(s) 24
SatI GCNGC 4 cut(s) 368, 632, 739, 2914
Sau96I GGNCC 5 cut(s) 170, 965, 2439, 2709, 3386
SchI GAGTC 5 cut(s) 191, 1520, 2056, 2312, 3290
ScrFI CCNGG 8 cut(s) 149, 247, 969, 1401, 1527, 3000, 3275, 3390
SduI GDGCHC 1 cut(s) 3132
SfaNI GCATC 5 cut(s) 666, 1018, 1455, 2204, 2712
SfcI CTRYAG 4 cut(s) 1298, 1953, 2520, 3054
Sfr274I CTCGAG 2 cut(s) 696, 2051
SinI GGWCC 3 cut(s) 965, 2439, 2709
SlaI CTCGAG 2 cut(s) 696, 2051
SmiMI CAYNNNNRTG 3 cut(s) 247, 584, 1613
SmlI CTYRAG 7 cut(s) 199, 696, 1421, 1771, 1946, 2051, 3362
SmoI CTYRAG 7 cut(s) 199, 696, 1421, 1771, 1946, 2051, 3362
SnaBI TACGTA 1 cut(s) 522
SphI GCATGC 1 cut(s) 670
SseBI AGGCCT 2 cut(s) 1719, 3393
SsiI CCGC 7 cut(s) 73, 380, 456, 595, 629, 670, 874
SspI AATATT 2 cut(s) 2059, 2429
SspMI CTAG 8 cut(s) 422, 539, 782, 1379, 1394, 2451, 2967, 3443
StuI AGGCCT 2 cut(s) 1719, 3393
StyD4I CCNGG 8 cut(s) 147, 245, 967, 1399, 1525, 2998, 3273, 3388
StyI CCWWGG 2 cut(s) 661, 1608
TaaI ACNGT 6 cut(s) 376, 451, 1461, 3055, 3134, 3350
TaiI ACGT 5 cut(s) 516, 524, 1704, 2410, 3421
TaqI TCGA 8 cut(s) 25, 697, 712, 1463, 1507, 2052, 2344, 3031
TaqII GACCGA 1 cut(s) 953
TatI WGTACW 2 cut(s) 1341, 3118
TscAI CASTG 3 cut(s) 2241, 2626, 2826
TseFI GTSAC 3 cut(s) 1366, 2615, 2971
TseI GCWGC 4 cut(s) 367, 631, 738, 2913
Tsp45I GTSAC 3 cut(s) 1366, 2615, 2971
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 3 cut(s) 2241, 2626, 2826
Tth111I GACNNNGTC 1 cut(s) 540
Van91I CCANNNNNTGG 1 cut(s) 1218
Vha464I CTTAAG 1 cut(s) 1771
VpaK11BI GGWCC 3 cut(s) 965, 2439, 2709
VspI ATTAAT 1 cut(s) 2733
XapI RAATTY 8 cut(s) 229, 714, 1666, 1789, 1864, 2578, 2593, 3009
XbaI TCTAGA 1 cut(s) 2450
XceI RCATGY 2 cut(s) 440, 670
XhoI CTCGAG 2 cut(s) 696, 2051
XmiI GTMKAC 4 cut(s) 25, 524, 3057, 3231
XmnI GAANNNNTTC 6 cut(s) 589, 1068, 1103, 1500, 2982, 3540
XspI CTAG 8 cut(s) 422, 539, 782, 1379, 1394, 2451, 2967, 3443
Zsp2I ATGCAT 1 cut(s) 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.