RchiOBHm_Chr1g0323761

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Forward (+)
11221771 .. 11227133
5363 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55363

Sequence Viewer

Length: 3573 bp
ATGGCGTCAACCTCTTCTCGTCGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGGGGGGAAGATACCCGCTACAATTTTACCGATCGTCTGTATGATGCACTAAGTGAGAAAGGAATTCTGACCTTTATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGCAGTCTAGATCTGCCATTGTCATTCTCTCACTCAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGACATGGGCCAACAAGTCCTCCCCGTCTTCTACACGGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAATGGGAACCCAAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATGACGACAGACTAAATGCCTGGAGAGCTGCTTTGACTGAGGTTGCCAATCTTTCTGGCTGGGATGCCAAGAATTATAAATTCGACTCAATAATATGTAAGGAAATTGTTAATCATATGTTAAAGAAGTCCATGTGTACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTCATGATTTATTAAGAAATTACATATGTCCGCAGCTTGTTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTGATGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAACCAATGGCCTAGTCTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTGGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAAAATTATCATAACCACCACAGATGTTCAGTTGTTAAAAGCACATGATGTTGATGCTACATACAAGGTTAAAGGGTTAAAATGTGATGAAGCACTTCAACTTTTGAGTTCGAAGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGCATCTGTGCAACCTTATTTTAGGATATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCACATAAACGAATCATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATCGATGTCTCATAACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAACAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTCATGAAGAGACATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTCAACTAAATTACAAGTGGCTGATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTTGTAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTCAGATATCTCCCACAAAATTTTGAAGCAGATGAACTAATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCTATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAGTCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAAATTCCCCAGTTTGTTGGAAATATGGAACGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGAAACTGCCTGTGTCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTGAGCAACTGCAAAAATCTTGTCTGTCTTCCGAGCACCATCAATAAATTGAAGTCTATTAGAAATCTTAATCTTTCGGGATGCTTGAAACTTGGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAAATGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCTCATAAAATATGTGAGATGTTCAGTCTTTCGTGGATGCGAAGTAGTTTGGGGATCTGTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAATGAGTTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCATCTGGCATTAGATTGCTTTCTAAGCTTGAGAACTTCAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAAGGCAGGTGGTTGTACTTCACTGAAATACCTGTTTGATGCATCAAATTTGAACGGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCATATGGTAATTCCAGGAAGTAATATTCCTGAGTGGTTCAGCCATCGGAGTGTTGGGTGTTCATTAAGTATGGGACCTGCATGCTTAGATGAAGCTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCAGTGGATAAGCTTTATATGGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGTTCAATGGAATAGAATTGGAAGTATATGGCACACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAATCAGATCACCTGTGGCTATTCTATGTATCTAGTGATAAGTACTTTGGTACAGAGAGGTGGCGTAACTGTTACGGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAGGGAGTGTGGAGTCCGTCTGATATATGAGGAAGATGTGCAAGCGTTGAACCAAACAACCACTCAATCAAGCAGCAGGATGTCTCCTTATGAGGATATATTGATTGGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGCCACTGGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1190

Amino Acids

134.89

Weight (kDa)

6.12

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 190 1.2e-51 TIR domain
TIR_2 PF13676 15 - 111 2.3e-15 TIR domain
NB-ARC PF00931 216 - 370 1e-19 NB-ARC domain
NACHT PF05729 220 - 363 1.1e-06 NACHT domain
WHD_ROQ1 PF23282 437 - 508 7.9e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 637 - 730 9.9e-07 Leucine-rich repeat region
LRR_13 PF23286 697 - 782 1.7e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 708 - 777 2.9e-08 Leucine-rich repeat region
C-JID PF20160 1000 - 1146 3.9e-13 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 498
AarI CACCTGC 1 cut(s) 2798
Acc36I ACCTGC 3 cut(s) 752, 2798, 3073
AccB1I GGYRCC 1 cut(s) 345
AccB7I CCANNNNNTGG 2 cut(s) 1618, 3133
AccI GTMKAC 1 cut(s) 2791
AciI CCGC 7 cut(s) 73, 343, 601, 635, 676, 883, 2554
AclI AACGTT 1 cut(s) 2164
AclWI GGATC 7 cut(s) 323, 336, 658, 671, 933, 1377, 2497
AcuI CTGAAG 4 cut(s) 1765, 2963, 3218, 3410
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 110
AfaI GTAC 5 cut(s) 559, 2818, 3114, 3317, 3325
AfiI CCNNNNNNNGG 5 cut(s) 1415, 1618, 2567, 3133, 3521
AjnI CCWGG 5 cut(s) 440, 976, 1534, 3001, 3267
AjuI GAANNNNNNNTTGG 2 cut(s) 2723, 2755
Alw21I GWGCWC 3 cut(s) 369, 2288, 3126
AlwI GGATC 7 cut(s) 323, 336, 658, 671, 933, 1377, 2497
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 2 cut(s) 702, 2060
AoxI GGCC 6 cut(s) 170, 295, 784, 1726, 2233, 3380
ApeKI GCWGC 5 cut(s) 185, 449, 637, 866, 3468
ArsI GACNNNNNNTTYG 2 cut(s) 2256, 2288
AseI ATTAAT 1 cut(s) 2105
Asp700I GAANNNNTTC 7 cut(s) 595, 807, 1077, 1509, 1603, 2948, 2985
AspS9I GGNCC 5 cut(s) 295, 974, 1714, 3062, 3380
AsuC2I CCSGG 1 cut(s) 3384
AsuHPI GGTGA 4 cut(s) 2627, 2671, 3275, 3406
AsuII TTCGAA 1 cut(s) 1094
AvaI CYCGRG 2 cut(s) 702, 2060
AvaII GGWCC 3 cut(s) 974, 1714, 3062
BamHI GGATCC 2 cut(s) 328, 663
BanI GGYRCC 1 cut(s) 345
BanII GRGCYC 1 cut(s) 369
BauI CACGAG 1 cut(s) 1184
BbsI GAAGAC 6 cut(s) 307, 1572, 1685, 2270, 2687, 2951
Bbv12I GWGCWC 3 cut(s) 369, 2288, 3126
BbvI GCAGC 5 cut(s) 172, 436, 649, 853, 3480
BccI CCATC 9 cut(s) 820, 1295, 2007, 2297, 2365, 2707, 2788, 3039, 3135
BceAI ACGGC 1 cut(s) 2240
BcgI CGANNNNNNTGC 2 cut(s) 2041, 2075
BciT130I CCWGG 5 cut(s) 442, 978, 1536, 3003, 3269
BciVI GTATCC 1 cut(s) 1785
BcnI CCSGG 1 cut(s) 3384
BfaI CTAG 5 cut(s) 201, 788, 1388, 2795, 3306
BfuAI ACCTGC 3 cut(s) 752, 2798, 3073
BfuI GTATCC 1 cut(s) 1785
BglII AGATCT 2 cut(s) 203, 1966
BisI GCNGC 6 cut(s) 186, 344, 450, 638, 867, 3469
BlsI GCNGC 6 cut(s) 187, 345, 451, 639, 868, 3470
BmcAI AGTACT 1 cut(s) 3317
Bme1390I CCNGG 6 cut(s) 442, 978, 1536, 3003, 3269, 3384
Bme18I GGWCC 3 cut(s) 974, 1714, 3062
BmeT110I CYCGRG 2 cut(s) 702, 2060
BmgT120I GGNCC 5 cut(s) 295, 974, 1714, 3062, 3380
BmiI GGNNCC 5 cut(s) 330, 347, 378, 665, 3063
BmrFI CCNGG 6 cut(s) 442, 978, 1536, 3003, 3269, 3384
BmrI ACTGGG 2 cut(s) 1779, 2137
BmuI ACTGGG 2 cut(s) 1779, 2137
BpiI GAAGAC 6 cut(s) 307, 1572, 1685, 2270, 2687, 2951
BpmI CTGGAG 1 cut(s) 463
Bpu10I CCTNAGC 1 cut(s) 1164
Bpu14I TTCGAA 1 cut(s) 1094
BpuEI CTTGAG 4 cut(s) 170, 1771, 1976, 2747
BpuMI CCSGG 1 cut(s) 3384
Bsa29I ATCGAT 1 cut(s) 1438
BsaBI GATNNNNATC 2 cut(s) 924, 1368
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1563, 1919
BsaJI CCNNGG 4 cut(s) 667, 977, 1612, 2008
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 5 cut(s) 1415, 1618, 2567, 3133, 3521
Bse1I ACTGG 8 cut(s) 971, 1311, 1774, 2143, 3131, 3515, 3531, 3544
Bse8I GATNNNNATC 2 cut(s) 924, 1368
BseBI CCWGG 5 cut(s) 442, 978, 1536, 3003, 3269
BseCI ATCGAT 1 cut(s) 1438
BseDI CCNNGG 4 cut(s) 667, 977, 1612, 2008
BseJI GATNNNNATC 2 cut(s) 924, 1368
BseLI CCNNNNNNNGG 5 cut(s) 1415, 1618, 2567, 3133, 3521
BseMII CTCAG 6 cut(s) 450, 1178, 2219, 2243, 2588, 3009
BseNI ACTGG 8 cut(s) 971, 1311, 1774, 2143, 3131, 3515, 3531, 3544
BseRI GAGGAG 1 cut(s) 1748
BseXI GCAGC 5 cut(s) 172, 436, 649, 853, 3480
BseYI CCCAGC 2 cut(s) 480, 1500
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 6 cut(s) 172, 297, 786, 1728, 2235, 3382
BshNI GGYRCC 1 cut(s) 345
BshVI ATCGAT 1 cut(s) 1438
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 3 cut(s) 369, 2288, 3126
BsiHKCI CYCGRG 2 cut(s) 702, 2060
BsiSI CCGG 1 cut(s) 3383
BslFI GGGAC 2 cut(s) 1098, 3075
BslI CCNNNNNNNGG 5 cut(s) 1415, 1618, 2567, 3133, 3521
BsmFI GGGAC 2 cut(s) 1098, 3075
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 6 cut(s) 172, 297, 786, 1728, 2235, 3382
Bso31I GGTCTC 2 cut(s) 1563, 1919
BsoBI CYCGRG 2 cut(s) 702, 2060
Bsp119I TTCGAA 1 cut(s) 1094
Bsp1286I GDGCHC 3 cut(s) 369, 2288, 3126
Bsp1407I TGTACA 1 cut(s) 557
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 7 cut(s) 73, 343, 601, 635, 676, 883, 2554
BspANI GGCC 6 cut(s) 172, 297, 786, 1728, 2235, 3382
BspCNI CTCAG 6 cut(s) 451, 1177, 2220, 2244, 2589, 3010
BspDI ATCGAT 1 cut(s) 1438
BspHI TCATGA 3 cut(s) 607, 1561, 1581
BspLI GGNNCC 5 cut(s) 330, 347, 378, 665, 3063
BspMI ACCTGC 3 cut(s) 752, 2798, 3073
BspPI GGATC 7 cut(s) 323, 336, 658, 671, 933, 1377, 2497
BspT104I TTCGAA 1 cut(s) 1094
BspT107I GGYRCC 1 cut(s) 345
BspTNI GGTCTC 2 cut(s) 1563, 1919
BsrGI TGTACA 1 cut(s) 557
BsrI ACTGG 8 cut(s) 971, 1311, 1774, 2143, 3131, 3515, 3531, 3544
BssECI CCNNGG 4 cut(s) 667, 977, 1612, 2008
BssNAI GTATAC 1 cut(s) 2792
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1184
BssT1I CCWWGG 3 cut(s) 667, 1612, 2008
Bst1107I GTATAC 1 cut(s) 2792
Bst2BI CACGAG 1 cut(s) 1184
Bst2UI CCWGG 5 cut(s) 442, 978, 1536, 3003, 3269
Bst4CI ACNGT 6 cut(s) 325, 1470, 1521, 2378, 3344, 3350
Bst6I CTCTTC 2 cut(s) 19, 1580
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 1483, 3552
BstAUI TGTACA 1 cut(s) 557
BstBI TTCGAA 1 cut(s) 1094
BstC8I GCNNGC 9 cut(s) 674, 1345, 1400, 1942, 2558, 3070, 3240, 3438, 3557
BstDSI CCRYGG 1 cut(s) 667
BstEII GGTNACC 1 cut(s) 2659
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 6 cut(s) 446, 678, 1483, 2722, 3101, 3552
BstNI CCWGG 5 cut(s) 442, 978, 1536, 3003, 3269
BstNSI RCATGY 2 cut(s) 676, 3072
BstPI GGTNACC 1 cut(s) 2659
BstSCI CCNGG 6 cut(s) 440, 976, 1534, 3001, 3267, 3382
BstV1I GCAGC 5 cut(s) 172, 436, 649, 853, 3480
BstV2I GAAGAC 6 cut(s) 307, 1572, 1685, 2270, 2687, 2951
BstX2I RGATCY 5 cut(s) 203, 328, 663, 1966, 2489
BstXI CCANNNNNNTGG 1 cut(s) 2150
BstYI RGATCY 5 cut(s) 203, 328, 663, 1966, 2489
BstZ17I GTATAC 1 cut(s) 2792
Bsu15I ATCGAT 1 cut(s) 1438
BsuI GTATCC 1 cut(s) 1785
BsuRI GGCC 6 cut(s) 172, 297, 786, 1728, 2235, 3382
BsuTUI ATCGAT 1 cut(s) 1438
BtgI CCRYGG 1 cut(s) 667
BtsIMutI CAGTG 5 cut(s) 1218, 2822, 3138, 3537, 3538
BveI ACCTGC 3 cut(s) 752, 2798, 3073
Cac8I GCNNGC 9 cut(s) 674, 1345, 1400, 1942, 2558, 3070, 3240, 3438, 3557
CaiI CAGNNNCTG 1 cut(s) 866
CciI TCATGA 3 cut(s) 607, 1561, 1581
Cfr13I GGNCC 5 cut(s) 295, 974, 1714, 3062, 3380
ClaI ATCGAT 1 cut(s) 1438
CseI GACGC 1 cut(s) 149
Csp6I GTAC 5 cut(s) 558, 2817, 3113, 3316, 3324
CviQI GTAC 5 cut(s) 558, 2817, 3113, 3316, 3324
DraI TTTAAA 1 cut(s) 2802
DraIII CACNNNGTG 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 19, 1580
EarI CTCTTC 2 cut(s) 19, 1580
Ecl136II GAGCTC 1 cut(s) 367
Eco130I CCWWGG 3 cut(s) 667, 1612, 2008
Eco147I AGGCCT 2 cut(s) 172, 1728
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 2 cut(s) 1563, 1919
Eco32I GATATC 1 cut(s) 1787
Eco47I GGWCC 3 cut(s) 974, 1714, 3062
Eco53kI GAGCTC 1 cut(s) 367
Eco57I CTGAAG 4 cut(s) 1765, 2963, 3218, 3410
Eco88I CYCGRG 2 cut(s) 702, 2060
Eco91I GGTNACC 1 cut(s) 2659
EcoICRI GAGCTC 1 cut(s) 367
EcoO109I RGGNCCY 1 cut(s) 3062
EcoO65I GGTNACC 1 cut(s) 2659
EcoRI GAATTC 2 cut(s) 120, 3155
EcoRII CCWGG 5 cut(s) 440, 976, 1534, 3001, 3267
EcoRV GATATC 1 cut(s) 1787
EcoT14I CCWWGG 3 cut(s) 667, 1612, 2008
EcoT22I ATGCAT 3 cut(s) 284, 1479, 2845
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 3 cut(s) 667, 1612, 2008
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 2 cut(s) 1098, 3075
FauI CCCGC 3 cut(s) 80, 608, 669
FauNDI CATATG 3 cut(s) 537, 629, 2991
FblI GTMKAC 1 cut(s) 2791
Fnu4HI GCNGC 6 cut(s) 186, 344, 450, 638, 867, 3469
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 6 cut(s) 186, 344, 450, 638, 867, 3469
FspBI CTAG 5 cut(s) 201, 788, 1388, 2795, 3306
GluI GCNGC 6 cut(s) 186, 344, 450, 638, 867, 3469
GsaI CCCAGC 2 cut(s) 484, 1504
GsuI CTGGAG 1 cut(s) 463
HaeIII GGCC 6 cut(s) 172, 297, 786, 1728, 2235, 3382
HapII CCGG 1 cut(s) 3383
HgaI GACGC 1 cut(s) 149
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 4 cut(s) 9, 574, 933, 1632
HindII GTYRAC 4 cut(s) 9, 574, 933, 1632
HindIII AAGCTT 4 cut(s) 2024, 2723, 3081, 3140
HpaII CCGG 1 cut(s) 3383
HphI GGTGA 4 cut(s) 2627, 2671, 3275, 3406
Hpy166II GTNNAC 8 cut(s) 9, 558, 574, 933, 1517, 1632, 1714, 2792
Hpy8I GTNNAC 8 cut(s) 9, 558, 574, 933, 1517, 1632, 1714, 2792
Hpy99I CGWCG 2 cut(s) 24, 27
HpyCH4III ACNGT 6 cut(s) 325, 1470, 1521, 2378, 3344, 3350
HpyCH4IV ACGT 2 cut(s) 1543, 2164
HpyF10VI GCNNNNNNNGC 6 cut(s) 446, 678, 1483, 2722, 3101, 3552
HpySE526I ACGT 2 cut(s) 1543, 2164
Hsp92I GRCGYC 1 cut(s) 5
LmnI GCTCC 2 cut(s) 863, 2621
Lsp1109I GCAGC 5 cut(s) 172, 436, 649, 853, 3480
MaeI CTAG 5 cut(s) 201, 788, 1388, 2795, 3306
MaeII ACGT 2 cut(s) 1543, 2164
MaeIII GTNAC 5 cut(s) 1375, 2642, 2659, 3338, 3344
MfeI CAATTG 4 cut(s) 189, 938, 2217, 2887
MflI RGATCY 5 cut(s) 203, 328, 663, 1966, 2489
MhlI GDGCHC 3 cut(s) 369, 2288, 3126
MlyI GAGTC 6 cut(s) 500, 1637, 1749, 2004, 2074, 3417
MmeI TCCRAC 2 cut(s) 1482, 2131
Mph1103I ATGCAT 3 cut(s) 284, 1479, 2845
MroXI GAANNNNTTC 7 cut(s) 595, 807, 1077, 1509, 1603, 2948, 2985
MslI CAYNNNNRTG 4 cut(s) 590, 1280, 1622, 3036
MspI CCGG 1 cut(s) 3383
MspR9I CCNGG 6 cut(s) 442, 978, 1536, 3003, 3269, 3384
MunI CAATTG 4 cut(s) 189, 938, 2217, 2887
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 5 cut(s) 442, 978, 1536, 3003, 3269
MwoI GCNNNNNNNGC 6 cut(s) 446, 678, 1483, 2722, 3101, 3552
NciI CCSGG 1 cut(s) 3384
NcoI CCATGG 1 cut(s) 667
NdeI CATATG 3 cut(s) 537, 629, 2991
NlaIV GGNNCC 5 cut(s) 330, 347, 378, 665, 3063
NmuCI GTSAC 2 cut(s) 1375, 2659
NsiI ATGCAT 3 cut(s) 284, 1479, 2845
NspI RCATGY 2 cut(s) 676, 3072
NspV TTCGAA 1 cut(s) 1094
PaeI GCATGC 2 cut(s) 676, 3072
PaeR7I CTCGAG 2 cut(s) 702, 2060
PagI TCATGA 3 cut(s) 607, 1561, 1581
PaqCI CACCTGC 1 cut(s) 2798
PceI AGGCCT 2 cut(s) 172, 1728
PctI GAATGC 1 cut(s) 603
PdmI GAANNNNTTC 7 cut(s) 595, 807, 1077, 1509, 1603, 2948, 2985
PflMI CCANNNNNTGG 2 cut(s) 1618, 3133
PfoI TCCNGGA 1 cut(s) 3001
PkrI GCNGC 6 cut(s) 187, 345, 451, 639, 868, 3470
Ple19I CGATCG 1 cut(s) 91
PleI GAGTC 6 cut(s) 500, 1636, 1749, 2003, 2073, 3416
PpsI GAGTC 6 cut(s) 500, 1636, 1749, 2003, 2073, 3416
PpuMI RGGWCCY 1 cut(s) 3062
PshBI ATTAAT 1 cut(s) 2105
PsiI TTATAA 1 cut(s) 498
Psp124BI GAGCTC 1 cut(s) 369
Psp1406I AACGTT 1 cut(s) 2164
Psp5II RGGWCCY 1 cut(s) 3062
Psp6I CCWGG 5 cut(s) 440, 976, 1534, 3001, 3267
PspEI GGTNACC 1 cut(s) 2659
PspFI CCCAGC 2 cut(s) 480, 1500
PspGI CCWGG 5 cut(s) 440, 976, 1534, 3001, 3267
PspN4I GGNNCC 5 cut(s) 330, 347, 378, 665, 3063
PspPI GGNCC 5 cut(s) 295, 974, 1714, 3062, 3380
PspPPI RGGWCCY 1 cut(s) 3062
PspXI VCTCGAGB 1 cut(s) 702
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 5 cut(s) 203, 328, 663, 1966, 2489
PvuI CGATCG 1 cut(s) 91
RsaI GTAC 5 cut(s) 559, 2818, 3114, 3317, 3325
RsaNI GTAC 5 cut(s) 558, 2817, 3113, 3316, 3324
RseI CAYNNNNRTG 4 cut(s) 590, 1280, 1622, 3036
SacI GAGCTC 1 cut(s) 369
SatI GCNGC 6 cut(s) 186, 344, 450, 638, 867, 3469
Sau96I GGNCC 5 cut(s) 295, 974, 1714, 3062, 3380
ScaI AGTACT 1 cut(s) 3317
SchI GAGTC 6 cut(s) 500, 1637, 1749, 2004, 2074, 3417
ScrFI CCNGG 6 cut(s) 442, 978, 1536, 3003, 3269, 3384
SduI GDGCHC 3 cut(s) 369, 2288, 3126
Sfr274I CTCGAG 2 cut(s) 702, 2060
SfuI TTCGAA 1 cut(s) 1094
SinI GGWCC 3 cut(s) 974, 1714, 3062
SlaI CTCGAG 2 cut(s) 702, 2060
SmiMI CAYNNNNRTG 4 cut(s) 590, 1280, 1622, 3036
SmlI CTYRAG 6 cut(s) 149, 702, 1750, 1955, 2060, 2726
SmoI CTYRAG 6 cut(s) 149, 702, 1750, 1955, 2060, 2726
SphI GCATGC 2 cut(s) 676, 3072
SseBI AGGCCT 2 cut(s) 172, 1728
SsiI CCGC 7 cut(s) 73, 343, 601, 635, 676, 883, 2554
SspI AATATT 2 cut(s) 1329, 3013
SspMI CTAG 5 cut(s) 201, 788, 1388, 2795, 3306
SstI GAGCTC 1 cut(s) 369
StuI AGGCCT 2 cut(s) 172, 1728
StyD4I CCNGG 6 cut(s) 440, 976, 1534, 3001, 3267, 3382
StyI CCWWGG 3 cut(s) 667, 1612, 2008
TaaI ACNGT 6 cut(s) 325, 1470, 1521, 2378, 3344, 3350
TaiI ACGT 2 cut(s) 1546, 2167
TaqI TCGA 7 cut(s) 504, 703, 1094, 1438, 1472, 1761, 2061
TaqII GACCGA 1 cut(s) 753
TatI WGTACW 4 cut(s) 557, 2816, 3112, 3315
TauI GCSGC 1 cut(s) 346
TscAI CASTG 5 cut(s) 1225, 2829, 3138, 3538, 3544
TseFI GTSAC 2 cut(s) 1375, 2659
TseI GCWGC 5 cut(s) 185, 449, 637, 866, 3468
Tsp45I GTSAC 2 cut(s) 1375, 2659
TspGWI ACGGA 3 cut(s) 1164, 2871, 3401
TspRI CASTG 5 cut(s) 1225, 2829, 3138, 3538, 3544
Van91I CCANNNNNTGG 2 cut(s) 1618, 3133
VpaK11BI GGWCC 3 cut(s) 974, 1714, 3062
VspI ATTAAT 1 cut(s) 2105
XbaI TCTAGA 1 cut(s) 200
XceI RCATGY 2 cut(s) 676, 3072
XcmI CCANNNNNNNNNTGG 3 cut(s) 823, 2006, 3038
XhoI CTCGAG 2 cut(s) 702, 2060
XmiI GTMKAC 1 cut(s) 2791
XmnI GAANNNNTTC 7 cut(s) 595, 807, 1077, 1509, 1603, 2948, 2985
XspI CTAG 5 cut(s) 201, 788, 1388, 2795, 3306
ZrmI AGTACT 1 cut(s) 3317
Zsp2I ATGCAT 3 cut(s) 284, 1479, 2845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.