RchiOBHm_Chr1g0322041

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
9544902 .. 9549589
4688 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55206

Sequence Viewer

Length: 3351 bp
ATGTGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGAGGGGCCAACAAGTCTTCCCCGTTTTCTACATGGGCGTCGATCCTTCTGATGTGCGGCACCAAAGGCGAAGTTTTGAGCTCATCAAATGGGAGCACCAAGTAGATGTGGAAGTACGGGAACATGAAGAAGTTTATGGGAAGAATCATGATAGACTAAATGCGTGGAGAGCTGCTTTGACTGAAGTGGCCAATCTGTCTGGCTGGGATAACAAGAATTATGGATATGAACAAATACTAATCGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGGTTCCCGTATTGATAGTTTATTAAGCAAATACATATATCCGCAGCTTGATGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATGTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCCAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCTAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTCGTGTAGAATGAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGATACAATTAGAAAAATTGGCTGGAAATCGCAACTGGTTTGGTCCGGGGAGTAGAATTATCATAACCACCACAGATATTCAGTTGTTAAAGGCGCATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATTTCCCCCACCAGAAGATTATTTGCATCTGTGCTACCGTATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCGTTTTTATTTGGTAGAAGAACTGATCAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATTATGATCGTGTGATACAAATACTAGACTATTGCCAACTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAATGAACTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCCAAAGAGCCAGGCAAACGTAGTAGACTATGGTCTCATGAAGACATCAACAATGTGCTGAAAAAAAATATGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAATCCAGAAGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCATAATGTGGACCTTCCCGAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCAAAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGACAGGTTGAAGTTCATCAAACTCTGTCATTCTCAAAATATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAAAGTTTAGATCTTGAAGGATGTGAGAATTTGGTTAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAATCTCAAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAATGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGTGTCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTGAGCAAATGCAGAAATCTTGTCCGTCTTCCGAGCACCATCAATAAATTGAAGTCTATTAAAAATCTTGATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGAGATATATTGTTTTGAGGAAAATGATGTGAATAGTAGGTCTGCAATAGAAATGTCATCTTCCCATGATCTCATAAAATCTGTGAGAGGTTCAATCTTTCATGTATATGAAGTAGTTTGGGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATGCAGGGCCATCGAGTTTCCGCTTGCCTATTTCTGGTTTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTCCCAAAGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACCATTTTGTTCGCGTTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTATCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAATAGACTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAAGAAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCCAAGTGGTTCGGCCATCAGAGTGTTGGGTGTTCATTAAGTGTCCGTCTACCTGCAGATTGGAATAACAGTCGGTTTTTAGGATTTGCTTTGTGTGCTGATTTTGTACTCCGTGAGCACCATCGGGTGGATGAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGATCGATGGAAGAGAACTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAATCAAATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGCTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATACGAGCAAGAAGTGCAAGGCTTGAACCAAACAACCACTCAATCAAGCAGTAGGATGTCTCCTTATGCAGATATATTGATTGGTTTTGACATTCCAGTTGGAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACACTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1116

Amino Acids

127.33

Weight (kDa)

6.52

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 113 1e-16 TIR domain
NB-ARC PF00931 138 - 293 3.6e-17 NB-ARC domain
NACHT PF05729 143 - 237 2.1e-06 NACHT domain
WHD_ROQ1 PF23282 360 - 431 1.7e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 620 - 704 1.1e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 631 - 699 1.5e-09 Leucine-rich repeat region
C-JID PF20160 923 - 1072 1.4e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 521, 2845
AccB1I GGYRCC 1 cut(s) 105
AccB7I CCANNNNNTGG 2 cut(s) 1387, 2911
AccI GTMKAC 4 cut(s) 326, 1318, 2560, 2833
AccII CGCG 1 cut(s) 2463
AciI CCGC 5 cut(s) 103, 404, 445, 652, 2323
AclWI GGATC 5 cut(s) 83, 427, 440, 702, 2266
AcoI YGGCCR 2 cut(s) 234, 2797
AcsI RAATTY 9 cut(s) 878, 1444, 1567, 1642, 1753, 2405, 2617, 2933, 3038
AcuI CTGAAG 5 cut(s) 249, 2454, 2732, 2996, 3188
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 4 cut(s) 162, 2587, 2892, 3103
AfiI CCNNNNNNNGG 5 cut(s) 1184, 1387, 2336, 2911, 3299
AflII CTTAAG 1 cut(s) 1549
AjnI CCWGG 3 cut(s) 1303, 2770, 3045
AjuI GAANNNNNNNTTGG 2 cut(s) 2492, 2524
Alw21I GWGCWC 4 cut(s) 129, 144, 2057, 2904
Alw26I GTCTC 8 cut(s) 1296, 1332, 1455, 1516, 1688, 1952, 2518, 3261
AlwI GGATC 5 cut(s) 83, 427, 440, 702, 2266
AlwNI CAGNNNCTG 1 cut(s) 635
Ama87I CYCGRG 2 cut(s) 471, 1910
AoxI GGCC 8 cut(s) 52, 234, 553, 1495, 2002, 2309, 2797, 3158
ApeKI GCWGC 3 cut(s) 218, 406, 635
ApoI RAATTY 9 cut(s) 878, 1444, 1567, 1642, 1753, 2405, 2617, 2933, 3038
AseI ATTAAT 1 cut(s) 1874
Asp700I GAANNNNTTC 7 cut(s) 364, 576, 846, 1278, 1372, 2717, 2754
AspLEI GCGC 1 cut(s) 796
AspS9I GGNCC 5 cut(s) 52, 743, 1483, 2309, 3158
AsuC2I CCSGG 2 cut(s) 747, 3162
AsuHPI GGTGA 4 cut(s) 2396, 2440, 3053, 3184
AvaI CYCGRG 2 cut(s) 471, 1910
AvaII GGWCC 2 cut(s) 743, 1483
BalI TGGCCA 1 cut(s) 236
BamHI GGATCC 1 cut(s) 432
BanI GGYRCC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 129
BauI CACGAG 2 cut(s) 953, 3084
BbsI GAAGAC 5 cut(s) 55, 1341, 1454, 2039, 2720
Bbv12I GWGCWC 4 cut(s) 129, 144, 2057, 2904
BbvI GCAGC 3 cut(s) 205, 418, 622
BceAI ACGGC 1 cut(s) 2009
BcgI CGANNNNNNTGC 2 cut(s) 2295, 2329
BciT130I CCWGG 3 cut(s) 1305, 2772, 3047
BciVI GTATCC 1 cut(s) 1554
BclI TGATCA 2 cut(s) 484, 991
BcnI CCSGG 2 cut(s) 747, 3162
BcoDI GTCTC 8 cut(s) 1296, 1332, 1455, 1516, 1688, 1952, 2518, 3261
BfaI CTAG 3 cut(s) 557, 1157, 2564
BfmI CTRYAG 1 cut(s) 2838
BfrI CTTAAG 1 cut(s) 1549
BfuAI ACCTGC 2 cut(s) 521, 2845
BfuI GTATCC 1 cut(s) 1554
BglII AGATCT 3 cut(s) 1208, 1553, 1735
BisI GCNGC 4 cut(s) 104, 219, 407, 636
BlsI GCNGC 4 cut(s) 105, 220, 408, 637
Bme1390I CCNGG 5 cut(s) 747, 1305, 2772, 3047, 3162
Bme18I GGWCC 2 cut(s) 743, 1483
BmeT110I CYCGRG 2 cut(s) 471, 1910
BmgT120I GGNCC 5 cut(s) 52, 743, 1483, 2309, 3158
BmiI GGNNCC 4 cut(s) 53, 107, 367, 434
BmrFI CCNGG 5 cut(s) 747, 1305, 2772, 3047, 3162
BmrI ACTGGG 1 cut(s) 1548
BmsI GCATC 7 cut(s) 796, 913, 1233, 2090, 2599, 2621, 2675
BmuI ACTGGG 1 cut(s) 1548
BoxI GACNNNNGTC 1 cut(s) 1324
BpiI GAAGAC 5 cut(s) 55, 1341, 1454, 2039, 2720
Bpu10I CCTNAGC 1 cut(s) 933
BpuEI CTTGAG 4 cut(s) 1540, 1814, 2516, 3155
BpuMI CCSGG 2 cut(s) 747, 3162
Bsa29I ATCGAT 1 cut(s) 2982
BsaBI GATNNNNATC 2 cut(s) 693, 1137
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 2 cut(s) 1332, 1688
BsaJI CCNNGG 4 cut(s) 436, 746, 1381, 1777
Bsc4I CCNNNNNNNGG 5 cut(s) 1184, 1387, 2336, 2911, 3299
Bse1I ACTGG 7 cut(s) 740, 1080, 1543, 3000, 3293, 3309, 3322
Bse8I GATNNNNATC 2 cut(s) 693, 1137
BseBI CCWGG 3 cut(s) 1305, 2772, 3047
BseCI ATCGAT 1 cut(s) 2982
BseDI CCNNGG 4 cut(s) 436, 746, 1381, 1777
BseGI GGATG 9 cut(s) 19, 625, 1084, 1248, 1751, 2105, 2690, 2920, 3258
BseJI GATNNNNATC 2 cut(s) 693, 1137
BseLI CCNNNNNNNGG 5 cut(s) 1184, 1387, 2336, 2911, 3299
BseMII CTCAG 5 cut(s) 947, 1471, 1988, 2012, 2357
BseNI ACTGG 7 cut(s) 740, 1080, 1543, 3000, 3293, 3309, 3322
BseRI GAGGAG 1 cut(s) 1517
BseXI GCAGC 3 cut(s) 205, 418, 622
BseYI CCCAGC 2 cut(s) 249, 1269
Bsh1236I CGCG 1 cut(s) 2463
Bsh1285I CGRYCG 1 cut(s) 656
BshFI GGCC 8 cut(s) 54, 236, 555, 1497, 2004, 2311, 2799, 3160
BshNI GGYRCC 1 cut(s) 105
BshVI ATCGAT 1 cut(s) 2982
BsiEI CGRYCG 1 cut(s) 656
BsiHKAI GWGCWC 4 cut(s) 129, 144, 2057, 2904
BsiHKCI CYCGRG 2 cut(s) 471, 1910
BsiSI CCGG 2 cut(s) 746, 3161
BslI CCNNNNNNNGG 5 cut(s) 1184, 1387, 2336, 2911, 3299
BsmAI GTCTC 8 cut(s) 1296, 1332, 1455, 1516, 1688, 1952, 2518, 3261
BsmI GAATGC 2 cut(s) 2308, 2475
BsnI GGCC 8 cut(s) 54, 236, 555, 1497, 2004, 2311, 2799, 3160
Bso31I GGTCTC 2 cut(s) 1332, 1688
BsoBI CYCGRG 2 cut(s) 471, 1910
Bsp1286I GDGCHC 4 cut(s) 129, 144, 2057, 2904
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 5 cut(s) 103, 404, 445, 652, 2323
BspANI GGCC 8 cut(s) 54, 236, 555, 1497, 2004, 2311, 2799, 3160
BspCNI CTCAG 5 cut(s) 946, 1470, 1989, 2013, 2358
BspDI ATCGAT 1 cut(s) 2982
BspFNI CGCG 1 cut(s) 2463
BspHI TCATGA 2 cut(s) 193, 1330
BspLI GGNNCC 4 cut(s) 53, 107, 367, 434
BspMAI CTGCAG 1 cut(s) 2842
BspMI ACCTGC 2 cut(s) 521, 2845
BspPI GGATC 5 cut(s) 83, 427, 440, 702, 2266
BspT107I GGYRCC 1 cut(s) 105
BspTI CTTAAG 1 cut(s) 1549
BspTNI GGTCTC 2 cut(s) 1332, 1688
BsrI ACTGG 7 cut(s) 740, 1080, 1543, 3000, 3293, 3309, 3322
BssECI CCNNGG 4 cut(s) 436, 746, 1381, 1777
BssNAI GTATAC 1 cut(s) 327
BssNI GRCGYC 1 cut(s) 84
BssSI CACGAG 2 cut(s) 953, 3084
BssT1I CCWWGG 3 cut(s) 436, 1381, 1777
Bst1107I GTATAC 1 cut(s) 327
Bst2BI CACGAG 2 cut(s) 953, 3084
Bst2UI CCWGG 3 cut(s) 1305, 2772, 3047
Bst4CI ACNGT 6 cut(s) 836, 917, 1239, 1290, 2855, 3122
Bst6I CTCTTC 1 cut(s) 2983
BstACI GRCGYC 1 cut(s) 84
BstAFI CTTAAG 1 cut(s) 1549
BstAPI GCANNNNNTGC 3 cut(s) 1252, 3211, 3330
BstC8I GCNNGC 5 cut(s) 443, 1114, 1711, 2327, 3018
BstDEI CTNAG 7 cut(s) 933, 1457, 1997, 2021, 2366, 2441, 2490
BstDSI CCRYGG 1 cut(s) 436
BstEII GGTNACC 1 cut(s) 2428
BstF5I GGATG 9 cut(s) 19, 625, 1084, 1248, 1751, 2105, 2690, 2920, 3258
BstFNI CGCG 1 cut(s) 2463
BstHHI GCGC 1 cut(s) 796
BstMAI GTCTC 8 cut(s) 1296, 1332, 1455, 1516, 1688, 1952, 2518, 3261
BstMCI CGRYCG 1 cut(s) 656
BstMWI GCNNNNNNNGC 9 cut(s) 112, 215, 447, 1252, 2491, 2879, 3211, 3321, 3330
BstNI CCWGG 3 cut(s) 1305, 2772, 3047
BstNSI RCATGY 1 cut(s) 445
BstPAI GACNNNNGTC 1 cut(s) 1324
BstPI GGTNACC 1 cut(s) 2428
BstSCI CCNGG 5 cut(s) 745, 1303, 2770, 3045, 3160
BstSFI CTRYAG 1 cut(s) 2838
BstUI CGCG 1 cut(s) 2463
BstV1I GCAGC 3 cut(s) 205, 418, 622
BstV2I GAAGAC 5 cut(s) 55, 1341, 1454, 2039, 2720
BstX2I RGATCY 5 cut(s) 432, 1208, 1553, 1735, 2258
BstYI RGATCY 5 cut(s) 432, 1208, 1553, 1735, 2258
BstZ17I GTATAC 1 cut(s) 327
Bsu15I ATCGAT 1 cut(s) 2982
BsuI GTATCC 1 cut(s) 1554
BsuRI GGCC 8 cut(s) 54, 236, 555, 1497, 2004, 2311, 2799, 3160
BsuTUI ATCGAT 1 cut(s) 2982
BtgI CCRYGG 1 cut(s) 436
BtsCI GGATG 9 cut(s) 19, 625, 1084, 1248, 1751, 2105, 2690, 2920, 3258
BtsIMutI CAGTG 3 cut(s) 2591, 3315, 3316
BveI ACCTGC 2 cut(s) 521, 2845
Cac8I GCNNGC 5 cut(s) 443, 1114, 1711, 2327, 3018
CaiI CAGNNNCTG 1 cut(s) 635
CciI TCATGA 2 cut(s) 193, 1330
CfoI GCGC 1 cut(s) 796
Cfr13I GGNCC 5 cut(s) 52, 743, 1483, 2309, 3158
ClaI ATCGAT 1 cut(s) 2982
CseI GACGC 1 cut(s) 73
Csp6I GTAC 4 cut(s) 161, 2586, 2891, 3102
CviQI GTAC 4 cut(s) 161, 2586, 2891, 3102
DdeI CTNAG 7 cut(s) 933, 1457, 1997, 2021, 2366, 2441, 2490
DraI TTTAAA 1 cut(s) 2265
EaeI YGGCCR 2 cut(s) 234, 2797
Eam1104I CTCTTC 1 cut(s) 2983
EarI CTCTTC 1 cut(s) 2983
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 3 cut(s) 436, 1381, 1777
Eco147I AGGCCT 1 cut(s) 1497
Eco24I GRGCYC 1 cut(s) 129
Eco31I GGTCTC 2 cut(s) 1332, 1688
Eco47I GGWCC 2 cut(s) 743, 1483
Eco53kI GAGCTC 1 cut(s) 127
Eco57I CTGAAG 5 cut(s) 249, 2454, 2732, 2996, 3188
Eco88I CYCGRG 2 cut(s) 471, 1910
Eco91I GGTNACC 1 cut(s) 2428
EcoICRI GAGCTC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 2428
EcoRI GAATTC 1 cut(s) 2933
EcoRII CCWGG 3 cut(s) 1303, 2770, 3045
EcoT14I CCWWGG 3 cut(s) 436, 1381, 1777
EcoT22I ATGCAT 3 cut(s) 41, 1248, 2614
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 3 cut(s) 436, 1381, 1777
FalI AAGNNNNNCTT 2 cut(s) 561, 593
FauI CCCGC 1 cut(s) 438
FbaI TGATCA 2 cut(s) 484, 991
FblI GTMKAC 4 cut(s) 326, 1318, 2560, 2833
Fnu4HI GCNGC 4 cut(s) 104, 219, 407, 636
FokI GGATG 9 cut(s) 26, 632, 1091, 1255, 1758, 2112, 2697, 2927, 3265
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 4 cut(s) 104, 219, 407, 636
FspBI CTAG 3 cut(s) 557, 1157, 2564
GlaI GCGC 1 cut(s) 795
GluI GCNGC 4 cut(s) 104, 219, 407, 636
GsaI CCCAGC 2 cut(s) 253, 1273
HaeIII GGCC 8 cut(s) 54, 236, 555, 1497, 2004, 2311, 2799, 3160
HapII CCGG 2 cut(s) 746, 3161
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 796
Hin1I GRCGYC 1 cut(s) 84
Hin6I GCGC 1 cut(s) 794
HinP1I GCGC 1 cut(s) 794
HincII GTYRAC 2 cut(s) 343, 1401
HindII GTYRAC 2 cut(s) 343, 1401
HindIII AAGCTT 2 cut(s) 1793, 2492
HpaII CCGG 2 cut(s) 746, 3161
HphI GGTGA 4 cut(s) 2396, 2440, 3053, 3184
Hpy166II GTNNAC 8 cut(s) 327, 343, 1286, 1319, 1401, 1483, 2561, 2834
Hpy8I GTNNAC 8 cut(s) 327, 343, 1286, 1319, 1401, 1483, 2561, 2834
Hpy99I CGWCG 1 cut(s) 89
HpyCH4III ACNGT 6 cut(s) 836, 917, 1239, 1290, 2855, 3122
HpyCH4IV ACGT 2 cut(s) 1312, 2354
HpyF10VI GCNNNNNNNGC 9 cut(s) 112, 215, 447, 1252, 2491, 2879, 3211, 3321, 3330
HpyF3I CTNAG 7 cut(s) 933, 1457, 1997, 2021, 2366, 2441, 2490
HpySE526I ACGT 2 cut(s) 1312, 2354
Hsp92I GRCGYC 1 cut(s) 84
HspAI GCGC 1 cut(s) 794
Ksp22I TGATCA 2 cut(s) 484, 991
LmnI GCTCC 3 cut(s) 139, 632, 2390
Lsp1109I GCAGC 3 cut(s) 205, 418, 622
LweI GCATC 7 cut(s) 796, 913, 1233, 2090, 2599, 2621, 2675
MaeI CTAG 3 cut(s) 557, 1157, 2564
MaeII ACGT 2 cut(s) 1312, 2354
MaeIII GTNAC 2 cut(s) 2411, 2428
MfeI CAATTG 2 cut(s) 1986, 2656
MflI RGATCY 5 cut(s) 432, 1208, 1553, 1735, 2258
MhlI GDGCHC 4 cut(s) 129, 144, 2057, 2904
MlsI TGGCCA 1 cut(s) 236
MluNI TGGCCA 1 cut(s) 236
MlyI GAGTC 3 cut(s) 1518, 1843, 3195
MmeI TCCRAC 3 cut(s) 1251, 1900, 3277
Mox20I TGGCCA 1 cut(s) 236
Mph1103I ATGCAT 3 cut(s) 41, 1248, 2614
MroXI GAANNNNTTC 7 cut(s) 364, 576, 846, 1278, 1372, 2717, 2754
MscI TGGCCA 1 cut(s) 236
MslI CAYNNNNRTG 4 cut(s) 359, 1391, 2241, 2805
Msp20I TGGCCA 1 cut(s) 236
MspCI CTTAAG 1 cut(s) 1549
MspI CCGG 2 cut(s) 746, 3161
MspR9I CCNGG 5 cut(s) 747, 1305, 2772, 3047, 3162
MunI CAATTG 2 cut(s) 1986, 2656
Mva1269I GAATGC 2 cut(s) 2308, 2475
MvaI CCWGG 3 cut(s) 1305, 2772, 3047
MvnI CGCG 1 cut(s) 2463
MwoI GCNNNNNNNGC 9 cut(s) 112, 215, 447, 1252, 2491, 2879, 3211, 3321, 3330
NciI CCSGG 2 cut(s) 747, 3162
NcoI CCATGG 1 cut(s) 436
NlaIV GGNNCC 4 cut(s) 53, 107, 367, 434
NmeAIII GCCGAG 1 cut(s) 2091
NmuCI GTSAC 1 cut(s) 2428
NsiI ATGCAT 3 cut(s) 41, 1248, 2614
NspI RCATGY 1 cut(s) 445
PaeI GCATGC 1 cut(s) 445
PaeR7I CTCGAG 1 cut(s) 471
PagI TCATGA 2 cut(s) 193, 1330
PceI AGGCCT 1 cut(s) 1497
PctI GAATGC 2 cut(s) 2308, 2475
PdmI GAANNNNTTC 7 cut(s) 364, 576, 846, 1278, 1372, 2717, 2754
PflMI CCANNNNNTGG 2 cut(s) 1387, 2911
PfoI TCCNGGA 1 cut(s) 2770
PkrI GCNGC 4 cut(s) 105, 220, 408, 637
PleI GAGTC 3 cut(s) 1518, 1842, 3194
PpsI GAGTC 3 cut(s) 1518, 1842, 3194
PshAI GACNNNNGTC 1 cut(s) 1324
PshBI ATTAAT 1 cut(s) 1874
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 3 cut(s) 1303, 2770, 3045
PspEI GGTNACC 1 cut(s) 2428
PspFI CCCAGC 2 cut(s) 249, 1269
PspGI CCWGG 3 cut(s) 1303, 2770, 3045
PspN4I GGNNCC 4 cut(s) 53, 107, 367, 434
PspPI GGNCC 5 cut(s) 52, 743, 1483, 2309, 3158
PspXI VCTCGAGB 1 cut(s) 471
PstI CTGCAG 1 cut(s) 2842
PstNI CAGNNNCTG 1 cut(s) 635
PsuI RGATCY 5 cut(s) 432, 1208, 1553, 1735, 2258
RsaI GTAC 4 cut(s) 162, 2587, 2892, 3103
RsaNI GTAC 4 cut(s) 161, 2586, 2891, 3102
RseI CAYNNNNRTG 4 cut(s) 359, 1391, 2241, 2805
SacI GAGCTC 1 cut(s) 129
SatI GCNGC 4 cut(s) 104, 219, 407, 636
Sau96I GGNCC 5 cut(s) 52, 743, 1483, 2309, 3158
SchI GAGTC 3 cut(s) 1518, 1843, 3195
ScrFI CCNGG 5 cut(s) 747, 1305, 2772, 3047, 3162
SduI GDGCHC 4 cut(s) 129, 144, 2057, 2904
SfaNI GCATC 7 cut(s) 796, 913, 1233, 2090, 2599, 2621, 2675
SfcI CTRYAG 1 cut(s) 2838
Sfr274I CTCGAG 1 cut(s) 471
SinI GGWCC 2 cut(s) 743, 1483
SlaI CTCGAG 1 cut(s) 471
SmiMI CAYNNNNRTG 4 cut(s) 359, 1391, 2241, 2805
SmlI CTYRAG 6 cut(s) 471, 1519, 1549, 1829, 2495, 3134
SmoI CTYRAG 6 cut(s) 471, 1519, 1549, 1829, 2495, 3134
SphI GCATGC 1 cut(s) 445
SseBI AGGCCT 1 cut(s) 1497
SsiI CCGC 5 cut(s) 103, 404, 445, 652, 2323
SspI AATATT 3 cut(s) 1098, 1687, 2782
SspMI CTAG 3 cut(s) 557, 1157, 2564
SstI GAGCTC 1 cut(s) 129
StuI AGGCCT 1 cut(s) 1497
StyD4I CCNGG 5 cut(s) 745, 1303, 2770, 3045, 3160
StyI CCWWGG 3 cut(s) 436, 1381, 1777
TaaI ACNGT 6 cut(s) 836, 917, 1239, 1290, 2855, 3122
TaiI ACGT 2 cut(s) 1315, 2357
TaqI TCGA 7 cut(s) 87, 288, 472, 1530, 2315, 2479, 2982
TaqII GACCGA 1 cut(s) 522
TatI WGTACW 2 cut(s) 2585, 2890
TauI GCSGC 1 cut(s) 106
TscAI CASTG 3 cut(s) 2598, 3316, 3322
TseFI GTSAC 1 cut(s) 2428
TseI GCWGC 3 cut(s) 218, 406, 635
Tsp45I GTSAC 1 cut(s) 2428
TspGWI ACGGA 6 cut(s) 310, 933, 2033, 2819, 2885, 3179
TspRI CASTG 3 cut(s) 2598, 3316, 3322
Van91I CCANNNNNTGG 2 cut(s) 1387, 2911
Vha464I CTTAAG 1 cut(s) 1549
VpaK11BI GGWCC 2 cut(s) 743, 1483
VspI ATTAAT 1 cut(s) 1874
XapI RAATTY 9 cut(s) 878, 1444, 1567, 1642, 1753, 2405, 2617, 2933, 3038
XceI RCATGY 1 cut(s) 445
XcmI CCANNNNNNNNNTGG 3 cut(s) 592, 1775, 2807
XhoI CTCGAG 1 cut(s) 471
XmiI GTMKAC 4 cut(s) 326, 1318, 2560, 2833
XmnI GAANNNNTTC 7 cut(s) 364, 576, 846, 1278, 1372, 2717, 2754
XspI CTAG 3 cut(s) 557, 1157, 2564
Zsp2I ATGCAT 3 cut(s) 41, 1248, 2614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.