Rw1G004700

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
9045680 .. 9049348
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004700.1

Sequence Viewer

Length: 2898 bp
ATGGCGTCAACCTCTTCTTGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGGGGGAAAGATACCCGCAAGAGTTTTACCTCTCTTCTGTATTCTGCACTAAATCGGAAAGGAATTCTGACCTTTATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGACTCTAGATCTGCCATTGTCATTCTCTCATGCAGATATGCTGAGTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCAGGAAAGACATGGGCCAAGAAGTCTTCCCCGTCTTCTACTTGGTGGATCCTTCTCATGTGCGGCACCAAACGGGATGTTATGAGCTCATCAAATGGAAAACCGAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGCAAGAATGAGGACAGACGAAATGCCTGGAAAGCCGCTTTGACAGAGGTGGCCAATCTTTCTGGCTGGGATTTCTTGTATTATCCATATCCAACAAACCTAGTTGACGAAATTGTTAATCACATGCTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATATATTAAGCAATTACATATATCCGCAGCTTGGTGGGGTGTGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAAGAATGGCCTAGTTTCTCTGCAAGAAAAACTTCTTTCCAGAATCCTGATGTCAGAAGTTAAAAATATAGAGGATGAATACACAGGAGCTGCTATGATACAAAGGCGGTTGTGTAGAAAAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTTCAGGGAGTAGAATTATCATAACCACCACAGATTATCAGTTGTTAAAGGCACATGAAGTTGATGCTACATACAAGGCTAGCGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAACGTCCCCCACCAGAAGATTATTTGCATCTGTGCTATGATATTATAGGGTATGCTAAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGAACTGATGAATGGGAAAGTGCGATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGATGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTCCTTGTTGATAGGTCTCTCATAACTATCTCCAACAATGAACTGTCCATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACTGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATAAAGACATCAACAATGTGCTGAAGAAAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTTGTAATGTGGACCTTCCCGACGGTCTCACTTGTCTTCCTAATTCCTTGAGACTCCTCGAATGGACAGGGTATCCCTTGACATCTCTCCCACAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGTCGAGTTGAAGTTCATCAAACTCTGCCATTCTCATAACATTGTGGAGACTCCAGACCTCGCAGGCGTCCAGAATCTTGAGAGTTTAGATCTGGAAGGGTGTGACAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAATGTTATGAAGATTCCCGAGTTTGGTGGAAATATGGAACGTTTGTTGGTTCTTTGTCTAGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATCGAACAGCTGAGCGGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAGTTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACAACAGGTAAATGTGGAAGTAATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCGCAGAAAATATGTGAGAGGTTCAATCTCTCATAGGTGCAAAGTAGTTTGGCGATCTTTAAAAAAGTTCTTGCTTTCTGGATTGGTGCGAAAAGTGAATACAGAGCCAAAGAGTTTTCACTTGCCTATATCACAAAAAGTGAATACAGAGCCAATGAGTTTCCACTTGCCTATATCACAAAAAGTGAATACAAAGCCAATGAGTTTCCGTTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAACTGCAATCTTGATGAAGGAGTATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTAACCTTGAATCTAAGTGGGAACAATTTTGTTCATCTTCCTTCAGGCATTGGATTGCTTTCTAGGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGGAAACTTTTCAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

965

Amino Acids

109.65

Weight (kDa)

7.05

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 190 2.3e-48 TIR domain
TIR_2 PF13676 14 - 107 1.4e-13 TIR domain
NB-ARC PF00931 217 - 371 2e-21 NB-ARC domain
AAA_22 PF13401 220 - 317 5e-06 AAA domain
NACHT PF05729 222 - 316 1.1e-07 NACHT domain
WHD_ROQ1 PF23282 437 - 508 6.5e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 697 - 782 4.1e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 708 - 777 6.9e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 752
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 1 cut(s) 1618
AccBSI CCGCTC 1 cut(s) 2232
AccI GTMKAC 1 cut(s) 557
AciI CCGC 9 cut(s) 70, 340, 450, 601, 635, 676, 883, 1056, 2232
AclI AACGTT 1 cut(s) 2164
AclWI GGATC 6 cut(s) 320, 333, 658, 671, 933, 1377
AcoI YGGCCR 1 cut(s) 465
AcsI RAATTY 6 cut(s) 117, 720, 1675, 1798, 1873, 2726
AcuI CTGAAG 2 cut(s) 1604, 2775
AcyI GRCGYC 2 cut(s) 5, 1944
AfiI CCNNNNNNNGG 5 cut(s) 641, 1415, 1618, 2147, 2657
AjnI CCWGG 2 cut(s) 440, 1534
AjuI GAANNNNNNNTTGG 2 cut(s) 2813, 2845
Alw21I GWGCWC 1 cut(s) 366
AlwI GGATC 6 cut(s) 320, 333, 658, 671, 933, 1377
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 3 cut(s) 702, 2060, 2141
AoxI GGCC 5 cut(s) 167, 292, 465, 784, 2233
ApeKI GCWGC 3 cut(s) 182, 637, 866
ApoI RAATTY 6 cut(s) 117, 720, 1675, 1798, 1873, 2726
AseI ATTAAT 1 cut(s) 2105
Asp700I GAANNNNTTC 6 cut(s) 595, 807, 1077, 1509, 1603, 2885
AspS9I GGNCC 2 cut(s) 292, 1714
AsuNHI GCTAGC 1 cut(s) 1052
AvaI CYCGRG 3 cut(s) 702, 2060, 2141
AvaII GGWCC 1 cut(s) 1714
BalI TGGCCA 1 cut(s) 467
BamHI GGATCC 2 cut(s) 325, 663
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1184
BbsI GAAGAC 5 cut(s) 295, 304, 1685, 1730, 2270
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 169, 649, 853
BccI CCATC 4 cut(s) 1295, 2007, 2297, 2878
BcgI CGANNNNNNTGC 2 cut(s) 2041, 2075
BciT130I CCWGG 2 cut(s) 442, 1536
BciVI GTATCC 1 cut(s) 1785
BclI TGATCA 1 cut(s) 715
BfaI CTAG 7 cut(s) 198, 515, 788, 1053, 1388, 2183, 2811
BfmI CTRYAG 1 cut(s) 2260
BfuAI ACCTGC 1 cut(s) 752
BfuI GTATCC 1 cut(s) 1785
BglII AGATCT 2 cut(s) 200, 1966
BisI GCNGC 6 cut(s) 183, 341, 450, 638, 867, 2233
BlpI GCTNAGC 1 cut(s) 2228
BlsI GCNGC 6 cut(s) 184, 342, 451, 639, 868, 2234
Bme1390I CCNGG 2 cut(s) 442, 1536
Bme18I GGWCC 1 cut(s) 1714
BmeT110I CYCGRG 3 cut(s) 702, 2060, 2141
BmgT120I GGNCC 2 cut(s) 292, 1714
BmiI GGNNCC 3 cut(s) 327, 344, 665
BmrFI CCNGG 2 cut(s) 442, 1536
BmsI GCATC 3 cut(s) 1027, 1144, 2321
BmtI GCTAGC 1 cut(s) 1056
BpiI GAAGAC 5 cut(s) 295, 304, 1685, 1730, 2270
BpmI CTGGAG 1 cut(s) 1914
Bpu10I CCTNAGC 1 cut(s) 1164
Bpu1102I GCTNAGC 1 cut(s) 2228
BpuEI CTTGAG 4 cut(s) 167, 1771, 1976, 2837
BsaBI GATNNNNATC 2 cut(s) 924, 1368
BsaHI GRCGYC 2 cut(s) 5, 1944
BsaI GGTCTC 3 cut(s) 1446, 1563, 1733
BsaJI CCNNGG 3 cut(s) 667, 1612, 2008
Bsc4I CCNNNNNNNGG 5 cut(s) 641, 1415, 1618, 2147, 2657
Bse1I ACTGG 2 cut(s) 971, 1311
Bse8I GATNNNNATC 2 cut(s) 924, 1368
BseBI CCWGG 2 cut(s) 442, 1536
BseDI CCNNGG 3 cut(s) 667, 1612, 2008
BseGI GGATG 6 cut(s) 139, 259, 359, 856, 1315, 2336
BseJI GATNNNNATC 2 cut(s) 924, 1368
BseLI CCNNNNNNNGG 5 cut(s) 641, 1415, 1618, 2147, 2657
BseMII CTCAG 3 cut(s) 225, 2219, 2678
BseNI ACTGG 2 cut(s) 971, 1311
BseRI GAGGAG 1 cut(s) 1748
BseXI GCAGC 3 cut(s) 169, 649, 853
BseYI CCCAGC 2 cut(s) 480, 1500
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 5 cut(s) 169, 294, 467, 786, 2235
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 3 cut(s) 702, 2060, 2141
BslFI GGGAC 1 cut(s) 1098
BslI CCNNNNNNNGG 5 cut(s) 641, 1415, 1618, 2147, 2657
BsmFI GGGAC 1 cut(s) 1098
BsnI GGCC 5 cut(s) 169, 294, 467, 786, 2235
Bso31I GGTCTC 3 cut(s) 1446, 1563, 1733
BsoBI CYCGRG 3 cut(s) 702, 2060, 2141
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 9 cut(s) 200, 325, 663, 715, 925, 1369, 1966, 2434, 2489
Bsp1720I GCTNAGC 1 cut(s) 2228
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 9 cut(s) 70, 340, 450, 601, 635, 676, 883, 1056, 2232
BspANI GGCC 5 cut(s) 169, 294, 467, 786, 2235
BspCNI CTCAG 3 cut(s) 226, 2220, 2679
BspLI GGNNCC 3 cut(s) 327, 344, 665
BspMAI CTGCAG 1 cut(s) 2264
BspMI ACCTGC 1 cut(s) 752
BspOI GCTAGC 1 cut(s) 1056
BspPI GGATC 6 cut(s) 320, 333, 658, 671, 933, 1377
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 3 cut(s) 1446, 1563, 1733
BsrBI CCGCTC 1 cut(s) 2232
BsrI ACTGG 2 cut(s) 971, 1311
BssECI CCNNGG 3 cut(s) 667, 1612, 2008
BssMI GATC 9 cut(s) 200, 325, 663, 715, 925, 1369, 1966, 2434, 2489
BssNAI GTATAC 1 cut(s) 558
BssNI GRCGYC 2 cut(s) 5, 1944
BssSI CACGAG 1 cut(s) 1184
BssT1I CCWWGG 3 cut(s) 667, 1612, 2008
Bst1107I GTATAC 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 1184
Bst2UI CCWGG 2 cut(s) 442, 1536
Bst4CI ACNGT 5 cut(s) 1067, 1470, 1521, 1727, 2378
Bst6I CTCTTC 2 cut(s) 19, 93
BstACI GRCGYC 2 cut(s) 5, 1944
BstAPI GCANNNNNTGC 1 cut(s) 1483
BstC8I GCNNGC 5 cut(s) 674, 1054, 1345, 1400, 1942
BstDEI CTNAG 8 cut(s) 47, 234, 1164, 1688, 2228, 2252, 2687, 2762
BstDSI CCRYGG 1 cut(s) 667
BstEII GGTNACC 1 cut(s) 2749
BstF5I GGATG 6 cut(s) 139, 259, 359, 856, 1315, 2336
BstKTI GATC 9 cut(s) 203, 328, 666, 718, 928, 1372, 1969, 2437, 2492
BstMBI GATC 9 cut(s) 200, 325, 663, 715, 925, 1369, 1966, 2434, 2489
BstMWI GCNNNNNNNGC 4 cut(s) 446, 678, 1483, 2812
BstNI CCWGG 2 cut(s) 442, 1536
BstNSI RCATGY 2 cut(s) 541, 676
BstPI GGTNACC 1 cut(s) 2749
BstSCI CCNGG 2 cut(s) 440, 1534
BstSFI CTRYAG 1 cut(s) 2260
BstV1I GCAGC 3 cut(s) 169, 649, 853
BstV2I GAAGAC 5 cut(s) 295, 304, 1685, 1730, 2270
BstX2I RGATCY 4 cut(s) 200, 325, 663, 1966
BstYI RGATCY 4 cut(s) 200, 325, 663, 1966
BstZ17I GTATAC 1 cut(s) 558
BsuI GTATCC 1 cut(s) 1785
BsuRI GGCC 5 cut(s) 169, 294, 467, 786, 2235
BtgI CCRYGG 1 cut(s) 667
BtsCI GGATG 6 cut(s) 139, 259, 359, 856, 1315, 2336
BveI ACCTGC 1 cut(s) 752
Cac8I GCNNGC 5 cut(s) 674, 1054, 1345, 1400, 1942
CaiI CAGNNNCTG 1 cut(s) 866
Cfr13I GGNCC 2 cut(s) 292, 1714
CseI GACGC 2 cut(s) 146, 1933
DdeI CTNAG 8 cut(s) 47, 234, 1164, 1688, 2228, 2252, 2687, 2762
DpnI GATC 9 cut(s) 202, 327, 665, 717, 927, 1371, 1968, 2436, 2491
DpnII GATC 9 cut(s) 200, 325, 663, 715, 925, 1369, 1966, 2434, 2489
DraI TTTAAA 1 cut(s) 2496
EaeI YGGCCR 1 cut(s) 465
Eam1104I CTCTTC 2 cut(s) 19, 93
EarI CTCTTC 2 cut(s) 19, 93
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 667, 1612, 2008
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 3 cut(s) 1446, 1563, 1733
Eco47I GGWCC 1 cut(s) 1714
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 2 cut(s) 1604, 2775
Eco88I CYCGRG 3 cut(s) 702, 2060, 2141
Eco91I GGTNACC 1 cut(s) 2749
EcoICRI GAGCTC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 2749
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 2 cut(s) 440, 1534
EcoT14I CCWWGG 3 cut(s) 667, 1612, 2008
EcoT22I ATGCAT 1 cut(s) 1479
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 667, 1612, 2008
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 1 cut(s) 1098
FauI CCCGC 4 cut(s) 77, 608, 669, 1049
FbaI TGATCA 1 cut(s) 715
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 6 cut(s) 183, 341, 450, 638, 867, 2233
FokI GGATG 6 cut(s) 146, 266, 366, 863, 1322, 2343
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 6 cut(s) 183, 341, 450, 638, 867, 2233
FspBI CTAG 7 cut(s) 198, 515, 788, 1053, 1388, 2183, 2811
GluI GCNGC 6 cut(s) 183, 341, 450, 638, 867, 2233
GsaI CCCAGC 2 cut(s) 484, 1504
GsuI CTGGAG 1 cut(s) 1914
HaeIII GGCC 5 cut(s) 169, 294, 467, 786, 2235
HgaI GACGC 2 cut(s) 146, 1933
Hin1I GRCGYC 2 cut(s) 5, 1944
HincII GTYRAC 5 cut(s) 9, 520, 574, 933, 1632
HindII GTYRAC 5 cut(s) 9, 520, 574, 933, 1632
HindIII AAGCTT 1 cut(s) 2024
Hpy166II GTNNAC 8 cut(s) 9, 520, 558, 574, 933, 1517, 1632, 1714
Hpy188I TCNGA 6 cut(s) 111, 123, 832, 1290, 2682, 2863
Hpy8I GTNNAC 8 cut(s) 9, 520, 558, 574, 933, 1517, 1632, 1714
Hpy99I CGWCG 1 cut(s) 1727
HpyAV CCTTC 9 cut(s) 339, 1090, 1354, 1727, 1855, 1967, 2702, 2799, 2876
HpyCH4III ACNGT 5 cut(s) 1067, 1470, 1521, 1727, 2378
HpyCH4IV ACGT 3 cut(s) 1111, 1543, 2164
HpyF10VI GCNNNNNNNGC 4 cut(s) 446, 678, 1483, 2812
HpyF3I CTNAG 8 cut(s) 47, 234, 1164, 1688, 2228, 2252, 2687, 2762
HpySE526I ACGT 3 cut(s) 1111, 1543, 2164
Hsp92I GRCGYC 2 cut(s) 5, 1944
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 9 cut(s) 200, 325, 663, 715, 925, 1369, 1966, 2434, 2489
LmnI GCTCC 1 cut(s) 863
Lsp1109I GCAGC 3 cut(s) 169, 649, 853
LweI GCATC 3 cut(s) 1027, 1144, 2321
MaeI CTAG 7 cut(s) 198, 515, 788, 1053, 1388, 2183, 2811
MaeII ACGT 3 cut(s) 1111, 1543, 2164
MaeIII GTNAC 5 cut(s) 1375, 1979, 2690, 2732, 2749
MalI GATC 9 cut(s) 202, 327, 665, 717, 927, 1371, 1968, 2436, 2491
MbiI CCGCTC 1 cut(s) 2232
MboI GATC 9 cut(s) 200, 325, 663, 715, 925, 1369, 1966, 2434, 2489
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 4 cut(s) 200, 325, 663, 1966
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 1 cut(s) 467
MluNI TGGCCA 1 cut(s) 467
MlyI GAGTC 5 cut(s) 188, 245, 1749, 1921, 2074
MmeI TCCRAC 2 cut(s) 530, 1482
Mox20I TGGCCA 1 cut(s) 467
Mph1103I ATGCAT 1 cut(s) 1479
MroXI GAANNNNTTC 6 cut(s) 595, 807, 1077, 1509, 1603, 2885
MscI TGGCCA 1 cut(s) 467
MslI CAYNNNNRTG 2 cut(s) 590, 1622
Msp20I TGGCCA 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 2227
MspR9I CCNGG 2 cut(s) 442, 1536
MunI CAATTG 1 cut(s) 186
MvaI CCWGG 2 cut(s) 442, 1536
MwoI GCNNNNNNNGC 4 cut(s) 446, 678, 1483, 2812
NcoI CCATGG 1 cut(s) 667
NdeII GATC 9 cut(s) 200, 325, 663, 715, 925, 1369, 1966, 2434, 2489
NheI GCTAGC 1 cut(s) 1052
NlaIV GGNNCC 3 cut(s) 327, 344, 665
NmeAIII GCCGAG 1 cut(s) 2322
NmuCI GTSAC 2 cut(s) 1375, 1979
NsiI ATGCAT 1 cut(s) 1479
NspI RCATGY 2 cut(s) 541, 676
PaeI GCATGC 1 cut(s) 676
PaeR7I CTCGAG 2 cut(s) 702, 2060
PceI AGGCCT 1 cut(s) 169
PdmI GAANNNNTTC 6 cut(s) 595, 807, 1077, 1509, 1603, 2885
PflMI CCANNNNNTGG 1 cut(s) 1618
PkrI GCNGC 6 cut(s) 184, 342, 451, 639, 868, 2234
PleI GAGTC 5 cut(s) 188, 244, 1749, 1921, 2073
PpsI GAGTC 5 cut(s) 188, 244, 1749, 1921, 2073
PshBI ATTAAT 1 cut(s) 2105
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 2164
Psp6I CCWGG 2 cut(s) 440, 1534
PspEI GGTNACC 1 cut(s) 2749
PspFI CCCAGC 2 cut(s) 480, 1500
PspGI CCWGG 2 cut(s) 440, 1534
PspN4I GGNNCC 3 cut(s) 327, 344, 665
PspPI GGNCC 2 cut(s) 292, 1714
PspXI VCTCGAGB 1 cut(s) 702
PstI CTGCAG 1 cut(s) 2264
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 4 cut(s) 200, 325, 663, 1966
PvuII CAGCTG 1 cut(s) 2227
RseI CAYNNNNRTG 2 cut(s) 590, 1622
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 6 cut(s) 183, 341, 450, 638, 867, 2233
Sau3AI GATC 9 cut(s) 200, 325, 663, 715, 925, 1369, 1966, 2434, 2489
Sau96I GGNCC 2 cut(s) 292, 1714
SchI GAGTC 5 cut(s) 188, 245, 1749, 1921, 2074
ScrFI CCNGG 2 cut(s) 442, 1536
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 3 cut(s) 1027, 1144, 2321
SfcI CTRYAG 1 cut(s) 2260
Sfr274I CTCGAG 2 cut(s) 702, 2060
SinI GGWCC 1 cut(s) 1714
SlaI CTCGAG 2 cut(s) 702, 2060
SmiMI CAYNNNNRTG 2 cut(s) 590, 1622
SmlI CTYRAG 6 cut(s) 146, 702, 1750, 1955, 2060, 2816
SmoI CTYRAG 6 cut(s) 146, 702, 1750, 1955, 2060, 2816
SphI GCATGC 1 cut(s) 676
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 9 cut(s) 70, 340, 450, 601, 635, 676, 883, 1056, 2232
SspI AATATT 1 cut(s) 1329
SspMI CTAG 7 cut(s) 198, 515, 788, 1053, 1388, 2183, 2811
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 2 cut(s) 440, 1534
StyI CCWWGG 3 cut(s) 667, 1612, 2008
TaaI ACNGT 5 cut(s) 1067, 1470, 1521, 1727, 2378
TaiI ACGT 3 cut(s) 1114, 1546, 2167
TaqI TCGA 5 cut(s) 703, 1761, 1881, 2061, 2220
TaqII GACCGA 1 cut(s) 753
TauI GCSGC 3 cut(s) 343, 452, 2235
TseFI GTSAC 2 cut(s) 1375, 1979
TseI GCWGC 3 cut(s) 182, 637, 866
Tsp45I GTSAC 2 cut(s) 1375, 1979
TspGWI ACGGA 3 cut(s) 541, 1164, 2633
Van91I CCANNNNNTGG 1 cut(s) 1618
VpaK11BI GGWCC 1 cut(s) 1714
VspI ATTAAT 1 cut(s) 2105
XapI RAATTY 6 cut(s) 117, 720, 1675, 1798, 1873, 2726
XbaI TCTAGA 2 cut(s) 197, 2182
XceI RCATGY 2 cut(s) 541, 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 2006
XhoI CTCGAG 2 cut(s) 702, 2060
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 6 cut(s) 595, 807, 1077, 1509, 1603, 2885
XspI CTAG 7 cut(s) 198, 515, 788, 1053, 1388, 2183, 2811
Zsp2I ATGCAT 1 cut(s) 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.