Rroxscaffold_4G00326870

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
59089356 .. 59095438
6083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326870.1

Sequence Viewer

Length: 2214 bp
ATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCGTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAAAAATATGGGAACAGATTCAATCCAAGGCATGGTAATAGAGTTGACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTCTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAACGTGGACCTTCCCAAAGGCCTCACCTGTCTTTCTAATTCCTTGAGACTTCTTGAATGGACTGGGTGTCCCTTAAGATCTCTCCCACAAAACTTTGATGCAGATGAACTTGTTGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGACGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTTCTCGTTTGGTAAGAATCCATCAATCCCTTGCATTTCTCAAAAAGCTTATTGTGCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAGTCTCTTGAATCATTAATTCTTTCTAACTGTTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAATGTTTGTTGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGATTCAATTGAACGCCTGAGTGGCCTTGTGTCATTGAATCTACGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAATTGAAGTCTATTAAAGATCTTAATCTTTCTGGATGCTTGAAACTGGGCAAACCTCAGGTAAATGTGGGGGTGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCACAAGAAAGAAACTGATGTGAATAGTGGATCTGCAATAGAAATGTCGTCTACCCATGATCGCAAGAAAAAAGGGAGAGGTTCAATCTTTCGAGGATGCAAAGTAGTTTGGCGATCTTTCAAAAAGTTCTTGCCTTCTGGATTGGTGCTAGAAGTGAATACAGAGCCAAAGAGCTTCCACTCGCCTATATCACAAAACGTGAATGCAGAGCCAAAGAGCTTCCCCTTGTCTGTATCTGGTCTGTGTAATTTAATAAATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGGAACAATTTTGTTCATCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAGATCATATTTCAATTTCATCAATTGCTTCAATCTAAACAGCAATCAAGGATGCAATGACATAGCATTCGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAGCAGGGAAACTTTTCAAATTGTATTTCCTGGAAGTAATATTCTTGAGTGGTTCAGTCATCAGAGTGTTGGGTGTTCTGTAAGTGTATCTCTACCTGCACATTGGAATAACAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTCCATGAGCACCATCAGGTGGATGAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTTGATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAATCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATGTGCATGAGTTCAACCAAACAACCACTCAATCAAGCAGTGGGATGTCTCCTTATGAGGATATGTTGATTGGTTTTGACAATCCAGTTGCAGGGGAAACCAGTGGCAGTGGTAGCGGAACTTGCACGCTTGAAGAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

737

Amino Acids

83.15

Weight (kDa)

5.86

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_3 PF07725 115 - 133 3.8e-07 Leucine Rich Repeat
LRR_14 PF23598 185 - 285 3.3e-10 Leucine-rich repeat region
LRR_13 PF23286 206 - 290 4.1e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 544 - 692 7e-18 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 458
Acc36I ACCTGC 2 cut(s) 458, 1708
AccB7I CCANNNNNTGG 2 cut(s) 145, 1774
AccI GTMKAC 2 cut(s) 1013, 1423
AciI CCGC 1 cut(s) 2187
AclWI GGATC 1 cut(s) 1000
AcsI RAATTY 6 cut(s) 202, 400, 1268, 1480, 1796, 1901
AcuI CTGAAG 5 cut(s) 131, 1317, 1595, 1859, 2051
AfaI GTAC 3 cut(s) 1450, 1755, 1966
AfiI CCNNNNNNNGG 3 cut(s) 145, 1774, 2162
AflII CTTAAG 1 cut(s) 307
AhdI GACNNNNNGTC 1 cut(s) 300
AjnI CCWGG 3 cut(s) 61, 1633, 2023
AjuI GAANNNNNNNTTGG 2 cut(s) 1355, 1387
AluBI AGCT 7 cut(s) 382, 553, 1137, 1182, 1357, 1783, 1843
AluI AGCT 7 cut(s) 382, 553, 1137, 1182, 1357, 1783, 1843
Alw21I GWGCWC 1 cut(s) 1767
AlwI GGATC 1 cut(s) 1000
Ama87I CYCGRG 2 cut(s) 587, 668
AoxI GGCC 3 cut(s) 253, 760, 2021
ApoI RAATTY 6 cut(s) 202, 400, 1268, 1480, 1796, 1901
ArsI GACNNNNNNTTYG 2 cut(s) 1547, 1579
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 4 cut(s) 36, 130, 1580, 1617
AspS9I GGNCC 2 cut(s) 241, 2021
AsuHPI GGTGA 6 cut(s) 250, 907, 1259, 1303, 1916, 2047
AsuII TTCGAA 1 cut(s) 1566
AvaI CYCGRG 2 cut(s) 587, 668
AvaII GGWCC 1 cut(s) 241
AxyI CCTNAGG 1 cut(s) 879
BauI CACGAG 1 cut(s) 1947
BbsI GAAGAC 4 cut(s) 99, 212, 797, 1583
Bbv12I GWGCWC 1 cut(s) 1767
BccI CCATC 7 cut(s) 534, 824, 892, 1420, 1436, 1776, 1841
BcgI CGANNNNNNTGC 2 cut(s) 568, 602
BciT130I CCWGG 3 cut(s) 63, 1635, 2025
BclI TGATCA 1 cut(s) 961
BfaI CTAG 2 cut(s) 1112, 1427
BfmI CTRYAG 1 cut(s) 787
BfrI CTTAAG 1 cut(s) 307
BfuAI ACCTGC 2 cut(s) 458, 1708
BglI GCCNNNNNGGC 1 cut(s) 759
BglII AGATCT 3 cut(s) 311, 493, 841
Bme1390I CCNGG 3 cut(s) 63, 1635, 2025
Bme18I GGWCC 1 cut(s) 241
BmeRI GACNNNNNGTC 1 cut(s) 300
BmeT110I CYCGRG 2 cut(s) 587, 668
BmgT120I GGNCC 2 cut(s) 241, 2021
BmrFI CCNGG 3 cut(s) 63, 1635, 2025
BmrI ACTGGG 2 cut(s) 306, 878
BmsI GCATC 6 cut(s) 322, 848, 1049, 1462, 1484, 1538
BmuI ACTGGG 2 cut(s) 306, 878
BpiI GAAGAC 4 cut(s) 99, 212, 797, 1583
Bpu14I TTCGAA 1 cut(s) 1566
BpuEI CTTGAG 4 cut(s) 298, 503, 1379, 1670
BsaBI GATNNNNATC 1 cut(s) 846
BsaI GGTCTC 2 cut(s) 90, 446
BsaJI CCNNGG 1 cut(s) 139
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 1774, 2162
Bse1I ACTGG 4 cut(s) 301, 873, 2156, 2172
Bse21I CCTNAGG 1 cut(s) 879
Bse3DI GCAATG 1 cut(s) 1558
Bse8I GATNNNNATC 1 cut(s) 846
BseBI CCWGG 3 cut(s) 63, 1635, 2025
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 6 cut(s) 509, 863, 1064, 1553, 1783, 2121
BseJI GATNNNNATC 1 cut(s) 846
BseLI CCNNNNNNNGG 3 cut(s) 145, 1774, 2162
BseMI GCAATG 1 cut(s) 1558
BseMII CTCAG 3 cut(s) 746, 893, 1220
BseNI ACTGG 4 cut(s) 301, 873, 2156, 2172
BseYI CCCAGC 1 cut(s) 27
BsgI GTGCAG 1 cut(s) 1686
BshFI GGCC 3 cut(s) 255, 762, 2023
BsiHKAI GWGCWC 1 cut(s) 1767
BsiHKCI CYCGRG 2 cut(s) 587, 668
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 3 cut(s) 145, 1774, 2162
BsmFI GGGAC 1 cut(s) 288
BsmI GAATGC 2 cut(s) 1171, 1562
BsnI GGCC 3 cut(s) 255, 762, 2023
Bso31I GGTCTC 2 cut(s) 90, 446
BsoBI CYCGRG 2 cut(s) 587, 668
Bsp119I TTCGAA 1 cut(s) 1566
Bsp1286I GDGCHC 1 cut(s) 1767
Bsp143I GATC 9 cut(s) 311, 493, 841, 961, 992, 1021, 1076, 1499, 1921
BspACI CCGC 1 cut(s) 2187
BspANI GGCC 3 cut(s) 255, 762, 2023
BspCNI CTCAG 3 cut(s) 747, 892, 1221
BspHI TCATGA 1 cut(s) 88
BspMAI CTGCAG 1 cut(s) 791
BspMI ACCTGC 2 cut(s) 458, 1708
BspPI GGATC 1 cut(s) 1000
BspT104I TTCGAA 1 cut(s) 1566
BspTI CTTAAG 1 cut(s) 307
BspTNI GGTCTC 2 cut(s) 90, 446
BsrDI GCAATG 1 cut(s) 1558
BsrI ACTGG 4 cut(s) 301, 873, 2156, 2172
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 9 cut(s) 311, 493, 841, 961, 992, 1021, 1076, 1499, 1921
BssNAI GTATAC 1 cut(s) 1424
BssSI CACGAG 1 cut(s) 1947
BssT1I CCWWGG 1 cut(s) 139
Bst1107I GTATAC 1 cut(s) 1424
Bst2BI CACGAG 1 cut(s) 1947
Bst2UI CCWGG 3 cut(s) 63, 1635, 2025
Bst4CI ACNGT 5 cut(s) 48, 647, 905, 1718, 1985
Bst6I CTCTTC 1 cut(s) 1846
BstAFI CTTAAG 1 cut(s) 307
BstAPI GCANNNNNTGC 2 cut(s) 10, 2074
BstBI TTCGAA 1 cut(s) 1566
BstC8I GCNNGC 2 cut(s) 1881, 2198
BstDEI CTNAG 6 cut(s) 215, 755, 879, 1229, 1304, 1353
BstEII GGTNACC 1 cut(s) 1291
BstF5I GGATG 6 cut(s) 509, 863, 1064, 1553, 1783, 2121
BstKTI GATC 9 cut(s) 314, 496, 844, 964, 995, 1024, 1079, 1502, 1924
BstMBI GATC 9 cut(s) 311, 493, 841, 961, 992, 1021, 1076, 1499, 1921
BstMWI GCNNNNNNNGC 8 cut(s) 10, 559, 759, 1354, 1742, 2074, 2184, 2193
BstNI CCWGG 3 cut(s) 63, 1635, 2025
BstPI GGTNACC 1 cut(s) 1291
BstSCI CCNGG 3 cut(s) 61, 1633, 2023
BstSFI CTRYAG 1 cut(s) 787
BstV2I GAAGAC 4 cut(s) 99, 212, 797, 1583
BstX2I RGATCY 4 cut(s) 311, 493, 841, 992
BstYI RGATCY 4 cut(s) 311, 493, 841, 992
BstZ17I GTATAC 1 cut(s) 1424
Bsu36I CCTNAGG 1 cut(s) 879
BsuRI GGCC 3 cut(s) 255, 762, 2023
BtsCI GGATG 6 cut(s) 509, 863, 1064, 1553, 1783, 2121
BtsI GCAGTG 2 cut(s) 2116, 2185
BtsIMutI CAGTG 4 cut(s) 1454, 2116, 2179, 2185
BveI ACCTGC 2 cut(s) 458, 1708
Cac8I GCNNGC 2 cut(s) 1881, 2198
CciI TCATGA 1 cut(s) 88
Cfr13I GGNCC 2 cut(s) 241, 2021
Csp6I GTAC 3 cut(s) 1449, 1754, 1965
CviAII CATG 8 cut(s) 5, 89, 145, 959, 1019, 1585, 1760, 2078
CviQI GTAC 3 cut(s) 1449, 1754, 1965
DdeI CTNAG 6 cut(s) 215, 755, 879, 1229, 1304, 1353
DpnI GATC 9 cut(s) 313, 495, 843, 963, 994, 1023, 1078, 1501, 1923
DpnII GATC 9 cut(s) 311, 493, 841, 961, 992, 1021, 1076, 1499, 1921
DriI GACNNNNNGTC 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 1846
Eam1105I GACNNNNNGTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 1846
Eco130I CCWWGG 1 cut(s) 139
Eco147I AGGCCT 1 cut(s) 255
Eco31I GGTCTC 2 cut(s) 90, 446
Eco47I GGWCC 1 cut(s) 241
Eco57I CTGAAG 5 cut(s) 131, 1317, 1595, 1859, 2051
Eco81I CCTNAGG 1 cut(s) 879
Eco88I CYCGRG 2 cut(s) 587, 668
Eco91I GGTNACC 1 cut(s) 1291
EcoO65I GGTNACC 1 cut(s) 1291
EcoRI GAATTC 1 cut(s) 1796
EcoRII CCWGG 3 cut(s) 61, 1633, 2023
EcoT14I CCWWGG 1 cut(s) 139
EcoT22I ATGCAT 2 cut(s) 6, 1477
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 8 cut(s) 8, 92, 148, 962, 1022, 1588, 1763, 2081
FaqI GGGAC 1 cut(s) 288
FatI CATG 8 cut(s) 4, 88, 144, 958, 1018, 1584, 1759, 2077
FbaI TGATCA 1 cut(s) 961
FblI GTMKAC 2 cut(s) 1013, 1423
FokI GGATG 6 cut(s) 516, 870, 1071, 1560, 1790, 2128
FspBI CTAG 2 cut(s) 1112, 1427
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 3 cut(s) 255, 762, 2023
Hin1II CATG 8 cut(s) 8, 92, 148, 962, 1022, 1588, 1763, 2081
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HindIII AAGCTT 3 cut(s) 551, 1355, 1841
HphI GGTGA 6 cut(s) 250, 907, 1259, 1303, 1916, 2047
Hpy166II GTNNAC 5 cut(s) 44, 159, 241, 1014, 1424
Hpy188I TCNGA 7 cut(s) 477, 1224, 1405, 1668, 1921, 2031, 2058
Hpy8I GTNNAC 5 cut(s) 44, 159, 241, 1014, 1424
HpyAV CCTTC 9 cut(s) 205, 254, 494, 1107, 1244, 1341, 1418, 2026, 2032
HpyCH4III ACNGT 5 cut(s) 48, 647, 905, 1718, 1985
HpyCH4IV ACGT 3 cut(s) 70, 237, 1161
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 559, 759, 1354, 1742, 2074, 2184, 2193
HpyF3I CTNAG 6 cut(s) 215, 755, 879, 1229, 1304, 1353
HpySE526I ACGT 3 cut(s) 70, 237, 1161
Hsp92II CATG 8 cut(s) 8, 92, 148, 962, 1022, 1588, 1763, 2081
Ksp22I TGATCA 1 cut(s) 961
Kzo9I GATC 9 cut(s) 311, 493, 841, 961, 992, 1021, 1076, 1499, 1921
LmnI GCTCC 1 cut(s) 1253
LweI GCATC 6 cut(s) 322, 848, 1049, 1462, 1484, 1538
MaeI CTAG 2 cut(s) 1112, 1427
MaeII ACGT 3 cut(s) 70, 237, 1161
MaeIII GTNAC 3 cut(s) 233, 1274, 1291
MalI GATC 9 cut(s) 313, 495, 843, 963, 994, 1023, 1078, 1501, 1923
MboI GATC 9 cut(s) 311, 493, 841, 961, 992, 1021, 1076, 1499, 1921
MboII GAAGA 9 cut(s) 104, 124, 212, 673, 797, 1319, 1588, 1863, 1910
MfeI CAATTG 2 cut(s) 744, 1519
MflI RGATCY 4 cut(s) 311, 493, 841, 992
MhlI GDGCHC 1 cut(s) 1767
MlyI GAGTC 3 cut(s) 601, 626, 2058
MmeI TCCRAC 1 cut(s) 658
Mph1103I ATGCAT 2 cut(s) 6, 1477
MroXI GAANNNNTTC 4 cut(s) 36, 130, 1580, 1617
MslI CAYNNNNRTG 1 cut(s) 1668
MspCI CTTAAG 1 cut(s) 307
MspR9I CCNGG 3 cut(s) 63, 1635, 2025
MunI CAATTG 2 cut(s) 744, 1519
Mva1269I GAATGC 2 cut(s) 1171, 1562
MvaI CCWGG 3 cut(s) 63, 1635, 2025
MwoI GCNNNNNNNGC 8 cut(s) 10, 559, 759, 1354, 1742, 2074, 2184, 2193
NdeII GATC 9 cut(s) 311, 493, 841, 961, 992, 1021, 1076, 1499, 1921
NlaIII CATG 8 cut(s) 8, 92, 148, 962, 1022, 1588, 1763, 2081
NmuCI GTSAC 1 cut(s) 1291
NsiI ATGCAT 2 cut(s) 6, 1477
NspV TTCGAA 1 cut(s) 1566
PaeR7I CTCGAG 1 cut(s) 587
PagI TCATGA 1 cut(s) 88
PaqCI CACCTGC 1 cut(s) 458
PceI AGGCCT 1 cut(s) 255
PctI GAATGC 2 cut(s) 1171, 1562
PdmI GAANNNNTTC 4 cut(s) 36, 130, 1580, 1617
PflMI CCANNNNNTGG 2 cut(s) 145, 1774
PfoI TCCNGGA 1 cut(s) 1633
PleI GAGTC 3 cut(s) 600, 625, 2057
PpsI GAGTC 3 cut(s) 600, 625, 2057
PshBI ATTAAT 1 cut(s) 632
Psp6I CCWGG 3 cut(s) 61, 1633, 2023
PspEI GGTNACC 1 cut(s) 1291
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 3 cut(s) 61, 1633, 2023
PspPI GGNCC 2 cut(s) 241, 2021
PstI CTGCAG 1 cut(s) 791
PsuI RGATCY 4 cut(s) 311, 493, 841, 992
RsaI GTAC 3 cut(s) 1450, 1755, 1966
RsaNI GTAC 3 cut(s) 1449, 1754, 1965
RseI CAYNNNNRTG 1 cut(s) 1668
Sau3AI GATC 9 cut(s) 311, 493, 841, 961, 992, 1021, 1076, 1499, 1921
Sau96I GGNCC 2 cut(s) 241, 2021
SchI GAGTC 3 cut(s) 601, 626, 2058
ScrFI CCNGG 3 cut(s) 63, 1635, 2025
SduI GDGCHC 1 cut(s) 1767
SfaNI GCATC 6 cut(s) 322, 848, 1049, 1462, 1484, 1538
SfcI CTRYAG 1 cut(s) 787
Sfr274I CTCGAG 1 cut(s) 587
SfuI TTCGAA 1 cut(s) 1566
SinI GGWCC 1 cut(s) 241
SlaI CTCGAG 1 cut(s) 587
SmiMI CAYNNNNRTG 1 cut(s) 1668
SmlI CTYRAG 6 cut(s) 277, 307, 482, 587, 1358, 1649
SmoI CTYRAG 6 cut(s) 277, 307, 482, 587, 1358, 1649
SseBI AGGCCT 1 cut(s) 255
SsiI CCGC 1 cut(s) 2187
SspI AATATT 1 cut(s) 1645
SspMI CTAG 2 cut(s) 1112, 1427
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 3 cut(s) 61, 1633, 2023
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 5 cut(s) 48, 647, 905, 1718, 1985
TaiI ACGT 3 cut(s) 73, 240, 1164
TaqI TCGA 3 cut(s) 588, 1054, 1566
TatI WGTACW 2 cut(s) 1448, 1753
TscAI CASTG 4 cut(s) 1461, 2116, 2179, 2185
TseFI GTSAC 1 cut(s) 1291
Tsp45I GTSAC 1 cut(s) 1291
TspDTI ATGAA 7 cut(s) 105, 354, 409, 1313, 1504, 1573, 1809
TspGWI ACGGA 1 cut(s) 2042
TspRI CASTG 4 cut(s) 1461, 2116, 2179, 2185
Van91I CCANNNNNTGG 2 cut(s) 145, 1774
Vha464I CTTAAG 1 cut(s) 307
VpaK11BI GGWCC 1 cut(s) 241
VspI ATTAAT 1 cut(s) 632
XapI RAATTY 6 cut(s) 202, 400, 1268, 1480, 1796, 1901
XhoI CTCGAG 1 cut(s) 587
XmiI GTMKAC 2 cut(s) 1013, 1423
XmnI GAANNNNTTC 4 cut(s) 36, 130, 1580, 1617
XspI CTAG 2 cut(s) 1112, 1427
Zsp2I ATGCAT 2 cut(s) 6, 1477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.