RchiOBHm_Chr6g0250871

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000105
Physical Location & Seq
Forward (+)
6184449 .. 6189616
5168 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22488

Sequence Viewer

Length: 3030 bp
ATGGATTCCCGCATTCATGATTTATTAAGCAATTACATATGTCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCCATCCATGATCGAATTTGTCAGGATTTTGACCAAAGCTGCATTCTTTCCAATGTTAGAGAAAAATCTGAAAAGGATGGCCTAGTTTCTCTTCAAGAAAAACTACTTTCCAGAATCCTAAGGACAAAAGTTGACATTGAGGATGAATATGCAGGAGCTACTATGATAGAAAGGCGGTTATGTAAACTAAAGGTGCTTGTTGTTATTGATGATGTGAATCACATCAATCAATTAGATAAATTGGCTGGAAGTCGGGACTGGTTCGGTCCTGGGAGTAGAATTATTGTAACCACCCCAGATATTCATTTGCTAAGGGGACATGATGTTGATGCTACATACAAGCCTACTGGGTTAAGTGATGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAAAAGCTTCCCACCAGAAGGTTATTTGGACTTGTGCCACCATATTATAGGGTATGCTCAGGGGCTTCCATTGGCTCTTGTGGTTCTAGGTTCTTTTCTATTTGGTAGAAGACCCAATGAATGGGAAAGTGCAATAGAGAGGCTAAACAATACACCAAATAGACAAGTTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGAAAAAGACCAACAGATTTTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGGCTATTGCAAGCTAGAACCTGGCATTGGTTTAAAGGTTCTTGAGGAAAAATCTCTCATTACTATTTTTGAGAACAAACTGTCAATGCATGATTTGCTACAAGAAATGGGCTTGGAAATAGTTCGTCGAGAGTCCCCTGATGAGCCAGGTAAACGAAGTAGATTATGGTCTCCTGAAGTTATCCATAATGTGCTGAAGAAAAATAAGGGAACAGACAAAATCCGAGCCATGGTAATGGACTTGCCTGAGTTACAAGTGGCTCATTGGAAACCAGAAGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCATAACGTGGATCTTCCCAAAGGCCTCACTTTTCTATCAAATTCCGTGAGACTCCTTGATTGGAGTGGGTACCCCTTAAGATCCCTCCCACAAAATTTTGAACCGGACGAACTTATTGAACTTAACTTGTGCCACAGCAACATTGAACACCTCTGGGAAGGAGCAAAGAATTTTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCAGACCCCAGACCTCACAGCCGTTCAAAATCTTGAGACTTTAGATCTTGAAGGATGTAAGACTTTGGTAAAACTTCATCAATCTGTCGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAAGATTGCGAGAGTCTCGAGAATCTGCCAAGTAAGATTGAAATGGAATCTCTTGAAACTTTAATTCTTTCTAACTGCTCCAAAGTTAAAAAGATTCCCGAGTTCGCTGGAAATATGAAGCGTTTATCAAAGCTTTATCTCAATGAGACTGCCATTGAAAAACTACCTGTGTCAATTGGATGCCTGAGTGGCCTTGCTTCACTGAATCTTAGCAACTGCAAAAATCTTCTATGCCTTGCAAGCACCTTAGAACTGCTTAGTAGATTGGAGTCTCTTAAAAAGCTTGATCTTTCTGGATGTTTGAAACTTCGAGAACAGGATAGTGTGAGGGAGAATGGTGCAATTAGAACTGCTGTAAATCATCTTATAAAACGTGGTTGTGAAGTTGGGAAATATTTGTCATGTTCCTTGCCTTCTAGATTGGTGCAAAGAGCCTATATGGAGCCAATGAGTATGAAGTTGCCTGTGTCTGATCTGTGTTATTCTACAGAGCAAAATGCAAATACATCAATGAGTATCCGGTTGCCACTATCTCGTCTATGGAATTTAACAGATCTAAACTTAAGTAACTGCAATCTTGGTGACACTGCATTTGCCAACTTTGCCTGTTTTCCCTCTTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTAAGCTTCAGAACATTAATTTGGAGAATTGCAAGACACTTCAAGAGTTGTCCGGACTTCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAAATGCTGTTTGATGTATCCAATTTCAATAGATTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTGCTGTAACATAGCATTAGAAATGCTGAGGATATTCCTTAATCAGCAAGTTTCTAGTGTGACGGAAACTTTTCAAATTGTAATTCCTGGAAGTGGAATTCCTGAGTGGTTTGATCATCGAAGATTAGCGAGTTCATTAACTGTAGACCTACTTCCAGATTGGAATGAGAGTAGGTTTTTGGGATTTGTTTTGTGTGCTGTTTTTGTACTCCATGAGCACCGTCAGGTTGATGAGCTTGATATCCATCAGTTCAAGACTTTTAAGGCCACTCATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTAGAAGTATACGGCAAACAGCCTGCATTTCGCTTTAGCGAACAATTTTGCCAGGTTGAGTCAGATCACCTTTGGATATTCTATGTGTCCCGTGATAAATACTTTGGTACAGATTGGTGGCATAACAGTTGCAGTCAACTTGAGTTCTTATTTGAAACAAGAGGGCCAGGCCTGAGGGTGAAGGAGTGTGGGGTACGTCTGATATATGAGCAAGATGTGCTAGAGTTGAACCAAACAATAACTCAATCAAGCAGTAGGATGTCTCCTCCATATGAGGATATATTGACTGATTTTGAGAATCAAGTTGAAGGGGAAACCATTCACCCAAACAAAAAGTTGAAAGGGGAAACTTGTGGTACTGGTAGTAGAACCTGCACCCTTGAAGAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1009

Amino Acids

114.47

Weight (kDa)

6.43

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 19 - 173 1.5e-16 NB-ARC domain
WHD_ROQ1 PF23282 239 - 310 1.3e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 439 - 581 3.6e-09 Leucine-rich repeat region
LRR_13 PF23286 499 - 589 9.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 804 - 952 8.2e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1817
Acc36I ACCTGC 1 cut(s) 3017
Acc65I GGTACC 1 cut(s) 1179
AccB1I GGYRCC 1 cut(s) 1179
AccB7I CCANNNNNTGG 1 cut(s) 633
AccI GTMKAC 2 cut(s) 2472, 2646
AccIII TCCGGA 1 cut(s) 2183
AciI CCGC 4 cut(s) 10, 44, 85, 289
AclWI GGATC 5 cut(s) 67, 80, 783, 1128, 1185
AcsI RAATTY 7 cut(s) 129, 1081, 1150, 1204, 1279, 1993, 2424
AcuI CTGAAG 5 cut(s) 677, 990, 1010, 2123, 2639
AfaI GTAC 6 cut(s) 758, 1181, 2535, 2746, 2832, 2995
AfiI CCNNNNNNNGG 6 cut(s) 50, 530, 633, 821, 1024, 2420
AflII CTTAAG 2 cut(s) 1186, 2011
AjnI CCWGG 6 cut(s) 382, 814, 940, 2413, 2688, 2803
AjuI GAANNNNNNNTTGG 6 cut(s) 141, 173, 804, 836, 2135, 2167
Alw21I GWGCWC 1 cut(s) 2547
Alw26I GTCTC 8 cut(s) 969, 1092, 1153, 1358, 1469, 1589, 1725, 2904
AlwI GGATC 5 cut(s) 67, 80, 783, 1128, 1185
AlwNI CAGNNNCTG 1 cut(s) 2127
Ama87I CYCGRG 3 cut(s) 111, 1466, 1547
Aor13HI TCCGGA 1 cut(s) 2183
AoxI GGCC 6 cut(s) 193, 1132, 1639, 2592, 2801, 2806
ApeKI GCWGC 3 cut(s) 46, 153, 2328
ApoI RAATTY 7 cut(s) 129, 1081, 1150, 1204, 1279, 1993, 2424
ArsI GACNNNNNNTTYG 2 cut(s) 2024, 2056
AseI ATTAAT 1 cut(s) 2148
Asp700I GAANNNNTTC 5 cut(s) 483, 518, 915, 2397, 2955
Asp718I GGTACC 1 cut(s) 1179
AspS9I GGNCC 3 cut(s) 380, 2124, 2801
AsuHPI GGTGA 5 cut(s) 488, 2042, 2696, 2827, 2951
AvaI CYCGRG 3 cut(s) 111, 1466, 1547
AvaII GGWCC 2 cut(s) 380, 2124
AxyI CCTNAGG 2 cut(s) 233, 2810
BamHI GGATCC 1 cut(s) 72
BanI GGYRCC 1 cut(s) 1179
BarI GAAGNNNNNNTAC 2 cut(s) 2463, 2495
BbsI GAAGAC 2 cut(s) 628, 1091
Bbv12I GWGCWC 1 cut(s) 2547
BbvCI CCTCAGC 1 cut(s) 2354
BbvI GCAGC 3 cut(s) 58, 140, 2315
BccI CCATC 7 cut(s) 125, 185, 467, 701, 2200, 2216, 2580
BceAI ACGGC 2 cut(s) 1334, 2665
BciT130I CCWGG 6 cut(s) 384, 816, 942, 2415, 2690, 2805
BciVI GTATCC 2 cut(s) 1976, 2266
BclI TGATCA 1 cut(s) 2440
BcoDI GTCTC 8 cut(s) 969, 1092, 1153, 1358, 1469, 1589, 1725, 2904
BfaI CTAG 8 cut(s) 197, 599, 713, 794, 809, 1866, 2382, 2858
BfmI CTRYAG 3 cut(s) 1935, 2469, 3026
BfrI CTTAAG 2 cut(s) 1186, 2011
BfuAI ACCTGC 1 cut(s) 3017
BfuI GTATCC 2 cut(s) 1976, 2266
BglI GCCNNNNNGGC 1 cut(s) 1638
BglII AGATCT 2 cut(s) 1372, 2002
BisI GCNGC 3 cut(s) 47, 154, 2329
BlsI GCNGC 3 cut(s) 48, 155, 2330
Bme1390I CCNGG 6 cut(s) 384, 816, 942, 2415, 2690, 2805
Bme18I GGWCC 2 cut(s) 380, 2124
BmeT110I CYCGRG 3 cut(s) 111, 1466, 1547
BmgT120I GGNCC 3 cut(s) 380, 2124, 2801
BmiI GGNNCC 3 cut(s) 74, 1181, 1893
BmrFI CCNGG 6 cut(s) 384, 816, 942, 2415, 2690, 2805
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 3 cut(s) 433, 1619, 2127
BmuI ACTGGG 1 cut(s) 471
BpiI GAAGAC 2 cut(s) 628, 1091
Bpu10I CCTNAGC 3 cut(s) 425, 570, 2354
BpuEI CTTGAG 3 cut(s) 857, 1382, 2798
BsaBI GATNNNNATC 2 cut(s) 330, 2571
BsaI GGTCTC 1 cut(s) 969
BsaJI CCNNGG 3 cut(s) 76, 383, 1023
BsaWI WCCGGW 3 cut(s) 1213, 1968, 2183
Bsc4I CCNNNNNNNGG 6 cut(s) 50, 530, 633, 821, 1024, 2420
Bse1I ACTGG 4 cut(s) 377, 466, 2241, 3001
Bse21I CCTNAGG 2 cut(s) 233, 2810
Bse8I GATNNNNATC 2 cut(s) 330, 2571
BseAI TCCGGA 1 cut(s) 2183
BseBI CCWGG 6 cut(s) 384, 816, 942, 2415, 2690, 2805
BseDI CCNNGG 3 cut(s) 76, 383, 1023
BseGI GGATG 9 cut(s) 117, 196, 262, 691, 1388, 1634, 1751, 2215, 2901
BseJI GATNNNNATC 2 cut(s) 330, 2571
BseLI CCNNNNNNNGG 6 cut(s) 50, 530, 633, 821, 1024, 2420
BseMII CTCAG 8 cut(s) 584, 1032, 1108, 1625, 2075, 2345, 2421, 2801
BseNI ACTGG 4 cut(s) 377, 466, 2241, 3001
BseRI GAGGAG 1 cut(s) 2892
BseXI GCAGC 3 cut(s) 58, 140, 2315
BsgI GTGCAG 1 cut(s) 2995
BshFI GGCC 6 cut(s) 195, 1134, 1641, 2594, 2803, 2808
BshNI GGYRCC 1 cut(s) 1179
BsiHKAI GWGCWC 1 cut(s) 2547
BsiHKCI CYCGRG 3 cut(s) 111, 1466, 1547
BsiSI CCGG 3 cut(s) 1214, 1969, 2184
BslFI GGGAC 4 cut(s) 383, 444, 913, 2710
BslI CCNNNNNNNGG 6 cut(s) 50, 530, 633, 821, 1024, 2420
BsmAI GTCTC 8 cut(s) 969, 1092, 1153, 1358, 1469, 1589, 1725, 2904
BsmFI GGGAC 4 cut(s) 383, 444, 913, 2710
BsmI GAATGC 2 cut(s) 12, 156
BsnI GGCC 6 cut(s) 195, 1134, 1641, 2594, 2803, 2808
Bso31I GGTCTC 1 cut(s) 969
BsoBI CYCGRG 3 cut(s) 111, 1466, 1547
Bsp1286I GDGCHC 1 cut(s) 2547
Bsp13I TCCGGA 1 cut(s) 2183
Bsp1407I TGTACA 1 cut(s) 756
Bsp19I CCATGG 2 cut(s) 76, 1023
BspACI CCGC 4 cut(s) 10, 44, 85, 289
BspANI GGCC 6 cut(s) 195, 1134, 1641, 2594, 2803, 2808
BspCNI CTCAG 8 cut(s) 583, 1033, 1107, 1626, 2076, 2346, 2422, 2802
BspEI TCCGGA 1 cut(s) 2183
BspHI TCATGA 1 cut(s) 16
BspLI GGNNCC 3 cut(s) 74, 1181, 1893
BspMI ACCTGC 1 cut(s) 3017
BspPI GGATC 5 cut(s) 67, 80, 783, 1128, 1185
BspT107I GGYRCC 1 cut(s) 1179
BspTI CTTAAG 2 cut(s) 1186, 2011
BspTNI GGTCTC 1 cut(s) 969
BsrGI TGTACA 1 cut(s) 756
BsrI ACTGG 4 cut(s) 377, 466, 2241, 3001
BssECI CCNNGG 3 cut(s) 76, 383, 1023
BssNAI GTATAC 1 cut(s) 2647
BssT1I CCWWGG 2 cut(s) 76, 1023
Bst1107I GTATAC 1 cut(s) 2647
Bst2UI CCWGG 6 cut(s) 384, 816, 942, 2415, 2690, 2805
Bst4CI ACNGT 4 cut(s) 876, 2470, 2549, 2765
Bst6I CTCTTC 2 cut(s) 210, 2626
BstAFI CTTAAG 2 cut(s) 1186, 2011
BstAPI GCANNNNNTGC 2 cut(s) 889, 2854
BstAUI TGTACA 1 cut(s) 756
BstC8I GCNNGC 4 cut(s) 83, 806, 1690, 2661
BstDSI CCRYGG 2 cut(s) 76, 1023
BstF5I GGATG 9 cut(s) 117, 196, 262, 691, 1388, 1634, 1751, 2215, 2901
BstMAI GTCTC 8 cut(s) 969, 1092, 1153, 1358, 1469, 1589, 1725, 2904
BstMWI GCNNNNNNNGC 6 cut(s) 87, 889, 1638, 1689, 2051, 2854
BstNI CCWGG 6 cut(s) 384, 816, 942, 2415, 2690, 2805
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 6 cut(s) 382, 814, 940, 2413, 2688, 2803
BstSFI CTRYAG 3 cut(s) 1935, 2469, 3026
BstV1I GCAGC 3 cut(s) 58, 140, 2315
BstV2I GAAGAC 2 cut(s) 628, 1091
BstX2I RGATCY 5 cut(s) 72, 1120, 1190, 1372, 2002
BstXI CCANNNNNNTGG 1 cut(s) 1030
BstYI RGATCY 5 cut(s) 72, 1120, 1190, 1372, 2002
BstZ17I GTATAC 1 cut(s) 2647
Bsu36I CCTNAGG 2 cut(s) 233, 2810
BsuI GTATCC 2 cut(s) 1976, 2266
BsuRI GGCC 6 cut(s) 195, 1134, 1641, 2594, 2803, 2808
BtgI CCRYGG 2 cut(s) 76, 1023
BtsCI GGATG 9 cut(s) 117, 196, 262, 691, 1388, 1634, 1751, 2215, 2901
BtsI GCAGTG 1 cut(s) 2034
BtsIMutI CAGTG 3 cut(s) 1649, 2034, 2234
BveI ACCTGC 1 cut(s) 3017
Cac8I GCNNGC 4 cut(s) 83, 806, 1690, 2661
CaiI CAGNNNCTG 1 cut(s) 2127
CciI TCATGA 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 380, 2124, 2801
Csp6I GTAC 6 cut(s) 757, 1180, 2534, 2745, 2831, 2994
CviAII CATG 9 cut(s) 17, 77, 82, 122, 434, 884, 1024, 1851, 2540
CviQI GTAC 6 cut(s) 757, 1180, 2534, 2745, 2831, 2994
DraI TTTAAA 2 cut(s) 501, 828
Eam1104I CTCTTC 2 cut(s) 210, 2626
EarI CTCTTC 2 cut(s) 210, 2626
Eco130I CCWWGG 2 cut(s) 76, 1023
Eco147I AGGCCT 2 cut(s) 1134, 2808
Eco31I GGTCTC 1 cut(s) 969
Eco32I GATATC 1 cut(s) 2569
Eco47I GGWCC 2 cut(s) 380, 2124
Eco57I CTGAAG 5 cut(s) 677, 990, 1010, 2123, 2639
Eco81I CCTNAGG 2 cut(s) 233, 2810
Eco88I CYCGRG 3 cut(s) 111, 1466, 1547
EcoO109I RGGNCCY 1 cut(s) 2124
EcoRI GAATTC 1 cut(s) 2424
EcoRII CCWGG 6 cut(s) 382, 814, 940, 2413, 2688, 2803
EcoRV GATATC 1 cut(s) 2569
EcoT14I CCWWGG 2 cut(s) 76, 1023
EcoT22I ATGCAT 1 cut(s) 885
ErhI CCWWGG 2 cut(s) 76, 1023
FaeI CATG 9 cut(s) 20, 80, 85, 125, 437, 887, 1027, 1854, 2543
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FaqI GGGAC 4 cut(s) 383, 444, 913, 2710
FatI CATG 9 cut(s) 16, 76, 81, 121, 433, 883, 1023, 1850, 2539
FauI CCCGC 2 cut(s) 17, 78
FauNDI CATATG 2 cut(s) 38, 2908
FbaI TGATCA 1 cut(s) 2440
FblI GTMKAC 2 cut(s) 2472, 2646
Fnu4HI GCNGC 3 cut(s) 47, 154, 2329
FokI GGATG 9 cut(s) 104, 203, 269, 698, 1395, 1641, 1758, 2222, 2908
Fsp4HI GCNGC 3 cut(s) 47, 154, 2329
FspBI CTAG 8 cut(s) 197, 599, 713, 794, 809, 1866, 2382, 2858
GluI GCNGC 3 cut(s) 47, 154, 2329
HaeIII GGCC 6 cut(s) 195, 1134, 1641, 2594, 2803, 2808
HapII CCGG 3 cut(s) 1214, 1969, 2184
Hin1II CATG 9 cut(s) 20, 80, 85, 125, 437, 887, 1027, 1854, 2543
HincII GTYRAC 2 cut(s) 247, 2774
HindII GTYRAC 2 cut(s) 247, 2774
HindIII AAGCTT 5 cut(s) 517, 1580, 1730, 2105, 2135
HpaII CCGG 3 cut(s) 1214, 1969, 2184
HphI GGTGA 5 cut(s) 488, 2042, 2696, 2827, 2951
Hpy166II GTNNAC 6 cut(s) 247, 299, 947, 2473, 2647, 2774
Hpy188I TCNGA 9 cut(s) 184, 696, 1019, 1418, 1921, 2085, 2142, 2701, 2838
Hpy8I GTNNAC 6 cut(s) 247, 299, 947, 2473, 2647, 2774
Hpy99I CGWCG 1 cut(s) 924
HpyAV CCTTC 7 cut(s) 524, 760, 1262, 1373, 1872, 2812, 2939
HpyCH4III ACNGT 4 cut(s) 876, 2470, 2549, 2765
HpyCH4IV ACGT 3 cut(s) 1116, 1822, 2833
HpyF10VI GCNNNNNNNGC 6 cut(s) 87, 889, 1638, 1689, 2051, 2854
HpySE526I ACGT 3 cut(s) 1116, 1822, 2833
Hsp92II CATG 9 cut(s) 20, 80, 85, 125, 437, 887, 1027, 1854, 2543
Kpn2I TCCGGA 1 cut(s) 2183
KpnI GGTACC 1 cut(s) 1183
Ksp22I TGATCA 1 cut(s) 2440
LmnI GCTCC 4 cut(s) 269, 1271, 1532, 1891
Lsp1109I GCAGC 3 cut(s) 58, 140, 2315
LweI GCATC 3 cut(s) 433, 1619, 2127
MaeI CTAG 8 cut(s) 197, 599, 713, 794, 809, 1866, 2382, 2858
MaeII ACGT 3 cut(s) 1116, 1822, 2833
MaeIII GTNAC 7 cut(s) 400, 781, 1044, 2015, 2030, 2333, 2386
MboII GAAGA 9 cut(s) 197, 633, 1003, 1091, 1115, 1667, 2223, 2460, 2643
MfeI CAATTG 2 cut(s) 1623, 2299
MflI RGATCY 5 cut(s) 72, 1120, 1190, 1372, 2002
MhlI GDGCHC 1 cut(s) 2547
MlyI GAGTC 5 cut(s) 935, 1155, 1471, 1727, 2705
MmeI TCCRAC 1 cut(s) 1396
Mph1103I ATGCAT 1 cut(s) 885
MroI TCCGGA 1 cut(s) 2183
MroXI GAANNNNTTC 5 cut(s) 483, 518, 915, 2397, 2955
MslI CAYNNNNRTG 1 cut(s) 1028
MspCI CTTAAG 2 cut(s) 1186, 2011
MspI CCGG 3 cut(s) 1214, 1969, 2184
MspR9I CCNGG 6 cut(s) 384, 816, 942, 2415, 2690, 2805
MunI CAATTG 2 cut(s) 1623, 2299
Mva1269I GAATGC 2 cut(s) 12, 156
MvaI CCWGG 6 cut(s) 384, 816, 942, 2415, 2690, 2805
MwoI GCNNNNNNNGC 6 cut(s) 87, 889, 1638, 1689, 2051, 2854
NcoI CCATGG 2 cut(s) 76, 1023
NdeI CATATG 2 cut(s) 38, 2908
NlaIII CATG 9 cut(s) 20, 80, 85, 125, 437, 887, 1027, 1854, 2543
NlaIV GGNNCC 3 cut(s) 74, 1181, 1893
NmuCI GTSAC 3 cut(s) 781, 2030, 2386
NsiI ATGCAT 1 cut(s) 885
NspI RCATGY 1 cut(s) 85
PaeI GCATGC 1 cut(s) 85
PaeR7I CTCGAG 2 cut(s) 111, 1466
PagI TCATGA 1 cut(s) 16
PceI AGGCCT 2 cut(s) 1134, 2808
PctI GAATGC 2 cut(s) 12, 156
PdmI GAANNNNTTC 5 cut(s) 483, 518, 915, 2397, 2955
PfeI GAWTC 9 cut(s) 5, 228, 331, 1444, 1471, 1496, 1543, 1654, 2935
PflMI CCANNNNNTGG 1 cut(s) 633
PfoI TCCNGGA 1 cut(s) 2413
PkrI GCNGC 3 cut(s) 48, 155, 2330
PleI GAGTC 5 cut(s) 934, 1155, 1470, 1726, 2704
PpsI GAGTC 5 cut(s) 934, 1155, 1470, 1726, 2704
PpuMI RGGWCCY 1 cut(s) 2124
PshBI ATTAAT 1 cut(s) 2148
PsiI TTATAA 1 cut(s) 1817
Psp5II RGGWCCY 1 cut(s) 2124
Psp6I CCWGG 6 cut(s) 382, 814, 940, 2413, 2688, 2803
PspGI CCWGG 6 cut(s) 382, 814, 940, 2413, 2688, 2803
PspN4I GGNNCC 3 cut(s) 74, 1181, 1893
PspPI GGNCC 3 cut(s) 380, 2124, 2801
PspPPI RGGWCCY 1 cut(s) 2124
PspXI VCTCGAGB 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 2127
PsuI RGATCY 5 cut(s) 72, 1120, 1190, 1372, 2002
RsaI GTAC 6 cut(s) 758, 1181, 2535, 2746, 2832, 2995
RsaNI GTAC 6 cut(s) 757, 1180, 2534, 2745, 2831, 2994
RseI CAYNNNNRTG 1 cut(s) 1028
SatI GCNGC 3 cut(s) 47, 154, 2329
Sau96I GGNCC 3 cut(s) 380, 2124, 2801
SchI GAGTC 5 cut(s) 935, 1155, 1471, 1727, 2705
ScrFI CCNGG 6 cut(s) 384, 816, 942, 2415, 2690, 2805
SduI GDGCHC 1 cut(s) 2547
SfaNI GCATC 3 cut(s) 433, 1619, 2127
SfcI CTRYAG 3 cut(s) 1935, 2469, 3026
Sfr274I CTCGAG 2 cut(s) 111, 1466
SinI GGWCC 2 cut(s) 380, 2124
SlaI CTCGAG 2 cut(s) 111, 1466
SmiMI CAYNNNNRTG 1 cut(s) 1028
SmlI CTYRAG 7 cut(s) 111, 836, 1186, 1361, 1466, 2011, 2777
SmoI CTYRAG 7 cut(s) 111, 836, 1186, 1361, 1466, 2011, 2777
SphI GCATGC 1 cut(s) 85
SseBI AGGCCT 2 cut(s) 1134, 2808
SsiI CCGC 4 cut(s) 10, 44, 85, 289
SspI AATATT 1 cut(s) 1844
SspMI CTAG 8 cut(s) 197, 599, 713, 794, 809, 1866, 2382, 2858
StuI AGGCCT 2 cut(s) 1134, 2808
StyD4I CCNGG 6 cut(s) 382, 814, 940, 2413, 2688, 2803
StyI CCWWGG 2 cut(s) 76, 1023
TaaI ACNGT 4 cut(s) 876, 2470, 2549, 2765
TaiI ACGT 3 cut(s) 1119, 1825, 2836
TaqI TCGA 6 cut(s) 112, 127, 922, 1467, 1759, 2446
TaqII GACCGA 1 cut(s) 368
TatI WGTACW 2 cut(s) 756, 2533
TfiI GAWTC 9 cut(s) 5, 228, 331, 1444, 1471, 1496, 1543, 1654, 2935
TscAI CASTG 3 cut(s) 1656, 2041, 2241
TseFI GTSAC 3 cut(s) 781, 2030, 2386
TseI GCWGC 3 cut(s) 46, 153, 2328
Tsp45I GTSAC 3 cut(s) 781, 2030, 2386
TspGWI ACGGA 2 cut(s) 1144, 2405
TspRI CASTG 3 cut(s) 1656, 2041, 2241
Van91I CCANNNNNTGG 1 cut(s) 633
Vha464I CTTAAG 2 cut(s) 1186, 2011
VpaK11BI GGWCC 2 cut(s) 380, 2124
VspI ATTAAT 1 cut(s) 2148
XapI RAATTY 7 cut(s) 129, 1081, 1150, 1204, 1279, 1993, 2424
XbaI TCTAGA 1 cut(s) 1865
XceI RCATGY 1 cut(s) 85
XhoI CTCGAG 2 cut(s) 111, 1466
XmiI GTMKAC 2 cut(s) 2472, 2646
XmnI GAANNNNTTC 5 cut(s) 483, 518, 915, 2397, 2955
XspI CTAG 8 cut(s) 197, 599, 713, 794, 809, 1866, 2382, 2858
Zsp2I ATGCAT 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.