Rmu_sc0004368.1_g000015

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004368.1
OG0000105
Physical Location & Seq
Forward (+)
70968 .. 75521
4554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004368.1_g000015.1.cds

Sequence Viewer

Length: 3867 bp
atgtgggggatgaggtcccgttcaagtggggggcaggggcctgctcgtcagccgacggcgcaggctgtgattggtgacggccgccgccgcgggggcaggaatcttggaaagcacgatggcggcggctggtttgatggagagtacgtcattgcggcggcggcggcggaggagaaggtggcggcgaaggagaaggtggcggcggcggtgaagttggcggcggcggtggaggcggcggtggcggcgaaggcggcggagagggtgtacccttctgattcaattccgaaggagatggtgtcgccgagaaggcgagatacgcgatggaagtacgatgtgtttctaagtttcagagggccagatacgcgcaaggggattacctccgagctgtatcatagactacaatggagaggaattaaaacctttatggatgaccccgatcttcgtgtaggagattccattagcccgtctcttgtttcagcaattgaggagtcaagatttgccattgtcattctttcacccaactatgcttcttcgccatggtgcttggatgaactcgtcaagattattcaatgcatgaaagagaaggggctacgagtcatgcccgttttctacaaagtggagccttctgatgtgcggcaccaaaggggaagttttgagctcaaagggaaaacccgaccccgagtagaagtaagggaacatgaagaagtttttggcaagaatgaggaccaattcaaggcgtggagagctgctttgagagaaatagccgatctttctgggtgggattcaaagaattttagaactgagagagaacttgttgaggcaattgttggggatattgggagtaaggtggtgtataaatcttcaagtgttgacaagggcttcgtaggaatggatgtccgtgttggtgttttattacataagtacatacatcaagagttggatgaggtacgaattatagggatacttgggatgcgaggagttggtaagacaacgcttgctcaagctttttatgatgactttggtcaggattatgagcatagatgctttctcttcaatgttagagaaaagtttaaaagggttgatggcctagtttctttacaaaagaaccttctttccagcattttgatggacaaatttgaatacatagaggatgaatatactggagctgctatgattaaaagaagtttatgtaaaaaaaaggtgctaattgttattgatgatgtggatcagttggcacaattacgaaacttggctggaagtcgcgactggttttgtccagggagcagaattatcataaccaccacggataatcacttgttagtggcacatggtgttgatgatacatacaaggctaccgggttaaattctggtgatgctcgtcaactcttgagtttaaaggctttcaagaaaagctccccaccagaaaattatttggacctgtgtgaccgtattttagggtatgctcaggggctgccgttagctcttgaggttttaggttcttttttatgtggtagagagcccaatgaatgggcaagtgcaattgataggctaaaaaatacaccagatagcggaattatgaatgtgcttcagattagttttgatggtctagaggacaaagagagagaaatattcctacatattgcttgtttttacaaggggcaagatagggatcgtgtgacacaaatactagactattgtgacctgaaccctgtcattggattacatgttcttgctgataaatctctcattactacttccagcaatgaactgtcaatgcatgatctgctacaagaaatgggctgggaaatagttcgtcgagagtctcgtgatgagccaggcaaacgtagtaggttatggtctcatgaagacatccataatatgttggagaaaaatgagggaacaaaagcaatccgtggcatggtaatggagttgcctaaattagaagtggctcattggaatccagaagccttctcaaatttgtctcaccttagtcttctccatattcgtaatgtggaccttcccaaaggcctcacttgtctttctaattccttgagactcctggaatggcctgggtgtccgttaagatctctcccacaaaagttcgaaccggacaaacttattgaacttaacttgtgtcacagcaatattaaacatctttggaaaggaagaaagaattttaaaaagttgaagatcatcaaactctgccattctcaaaacattgtggagactccagacctcacaggcgttccgaatcttgagagtttagaccttgaaggatgtaagagtttggtaagaatccatcaatcccttggatttctcaaaaagcttattgtcctgaaccttaaagactgcaaaagtcttgagaatctgccaggtagaattgaaatggaatctcttgaaacattaattctttctaactgctcaaaaattaaaaagattcccgagtttgatggaaatatggagcgcctgtctgtgcttcatcttgacaagactgctattgaggaactgcctgtttcaattgaacgcctttgtggccttgtgtcattgaatctacataactgcacaaatcttgtttttcttccaagcaccatcaataaattgaagtctgttgaaaatcttaatctttctggatgcataaaacttggcaaacatcaggaaagcgtgggggaggtggactgttatgaggaaaatgatgtgaatagtgggtctgcagtagaaatgtcatctacccgtgatctcataaaatatgtgagaggtccaatcattcatggatgcaaagtagtttgcgaatctttaaataagttcttgccttctggattggtgcaaaaagtgaatacagagccaatgagtgtccacttgcctatatcgggtctctgtaatttaacatgtctgaacctgagtaattgcaatcttggtgaaggagcatttgccaatgaatttggttactttccctctttggtgaccttgaatctaagtggaaacaattttattcgtcttccttcaggcattggattgctttcgaagcttgagaactttaacttggagaattgcaagagacttgaagagttgtcagaccttccatcaaatagtagcctagatttaagggcagatggttgtacgtcactgaaatacttgtttgatgcatcaaattttgacagattaaacatatcatatttcagtttcatgaattgcgtcaatctaaatggcaatcacggatgcaataacatagcatttgaaatgctgaagacattcatgtatcagggaatctctaatatgatggaaacttctcgaattgcaattcctggaagtaatattcctgagtggttcagccatcgacgtgttgggtgttcagtaagtgtagagctgcctcctgattgggatagcagtaggtttatgggatttgctttgtgtgttgtttttgtactccatgagcaccatcaggttgatgagcgtgatattcatcaattcaagacttttaacgctactcatcatcttgtatgttgcctgaagctcaatggaagagaattagaaatatacgacagacagcctgcatttcgctttagtgaagaattaggccaggttgagtcagatcacctgtggctattctatgtatctcgtgataaatactttggtacggagtggtggcataacagttgcagtcagcttgatttcttatttaaaaccagagggccgggtctgaaggtgaaggagtgtggagtccgtctgatatatgcgcaagatgtgcaagagttgaaccaaacaaccactcaatctagcagtccgtctgatatattgattgattttgacattccagttgaaggggaaaccagtggcactggtagcagaacttgcacgcttgaagaattgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1288

Amino Acids

145.16

Weight (kDa)

6.35

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 3732
AccB1I GGYRCC 1 cut(s) 633
AccB7I CCANNNNNTGG 1 cut(s) 1524
AccII CGCG 4 cut(s) 90, 316, 361, 1260
AclWI GGATC 2 cut(s) 1230, 1674
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 7 cut(s) 787, 1130, 1360, 1972, 2170, 2933, 3145
AcuI CTGAAG 5 cut(s) 1568, 2982, 3260, 3524, 3716
AdeI CACNNNGTG 1 cut(s) 1328
AfaI GTAC 8 cut(s) 143, 263, 326, 920, 945, 3115, 3420, 3631
AfiI CCNNNNNNNGG 8 cut(s) 91, 730, 1524, 1565, 1712, 1915, 2864, 3815
AflIII ACRYGT 3 cut(s) 1720, 2882, 3334
AjiI CACGTC 1 cut(s) 3335
AjnI CCWGG 7 cut(s) 1273, 1831, 2055, 2065, 2368, 3298, 3573
AjuI GAANNNNNNNTTGG 4 cut(s) 690, 722, 3020, 3052
AloI GAACNNNNNNTCC 4 cut(s) 1094, 1126, 1707, 1739
Alw21I GWGCWC 2 cut(s) 657, 3432
Alw26I GTCTC 8 cut(s) 468, 1824, 1860, 1983, 2044, 2216, 2873, 3046
AlwI GGATC 2 cut(s) 1230, 1674
AlwNI CAGNNNCTG 1 cut(s) 1468
Ama87I CYCGRG 2 cut(s) 675, 2438
AoxI GGCC 9 cut(s) 38, 79, 350, 1081, 2023, 2063, 2530, 3571, 3686
ApeKI GCWGC 4 cut(s) 743, 1163, 1468, 3361
ApoI RAATTY 7 cut(s) 787, 1130, 1360, 1972, 2170, 2933, 3145
ArsI GACNNNNNNTTYG 2 cut(s) 860, 892
AseI ATTAAT 1 cut(s) 2402
Asp700I GAANNNNTTC 2 cut(s) 1806, 3245
AspLEI GCGC 4 cut(s) 61, 363, 2463, 3733
AspS9I GGNCC 8 cut(s) 15, 38, 350, 721, 1432, 2011, 2753, 3686
AsuC2I CCSGG 2 cut(s) 1354, 3690
AsuHPI GGTGA 9 cut(s) 86, 217, 504, 1379, 1973, 2924, 2968, 3581, 3712
AsuII TTCGAA 2 cut(s) 2100, 3017
AvaI CYCGRG 2 cut(s) 675, 2438
AvaII GGWCC 5 cut(s) 15, 721, 1432, 2011, 2753
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 2 cut(s) 657, 1518
BarI GAAGNNNNNNTAC 2 cut(s) 295, 327
BauI CACGAG 2 cut(s) 1821, 3612
BbsI GAAGAC 4 cut(s) 1869, 1982, 2984, 3248
Bbv12I GWGCWC 2 cut(s) 657, 3432
BbvI GCAGC 4 cut(s) 730, 1150, 1455, 3348
BceAI ACGGC 3 cut(s) 72, 94, 1456
BciT130I CCWGG 7 cut(s) 1275, 1833, 2057, 2067, 2370, 3300, 3575
BciVI GTATCC 1 cut(s) 951
BcnI CCSGG 2 cut(s) 1354, 3690
BcoDI GTCTC 8 cut(s) 468, 1824, 1860, 1983, 2044, 2216, 2873, 3046
BfaI CTAG 5 cut(s) 1085, 1604, 1685, 3092, 3771
BfmI CTRYAG 1 cut(s) 2706
BfoI RGCGCY 1 cut(s) 2464
BfuI GTATCC 1 cut(s) 951
BglI GCCNNNNNGGC 3 cut(s) 93, 304, 2529
BglII AGATCT 1 cut(s) 2081
Bme1390I CCNGG 9 cut(s) 1275, 1354, 1833, 2057, 2067, 2370, 3300, 3575, 3690
Bme18I GGWCC 5 cut(s) 15, 721, 1432, 2011, 2753
BmeT110I CYCGRG 2 cut(s) 675, 2438
BmgBI CACGTC 1 cut(s) 3335
BmgT120I GGNCC 8 cut(s) 15, 38, 350, 721, 1432, 2011, 2753, 3686
BmiI GGNNCC 4 cut(s) 17, 39, 618, 635
BmrFI CCNGG 9 cut(s) 1275, 1354, 1833, 2057, 2067, 2370, 3300, 3575, 3690
BmsI GCATC 8 cut(s) 957, 1028, 1360, 2618, 2759, 3127, 3149, 3203
BoxI GACNNNNGTC 1 cut(s) 1017
BpiI GAAGAC 4 cut(s) 1869, 1982, 2984, 3248
BpmI CTGGAG 2 cut(s) 1179, 2211
Bpu10I CCTNAGC 1 cut(s) 1461
Bpu14I TTCGAA 2 cut(s) 2100, 3017
BpuEI CTTGAG 7 cut(s) 981, 1405, 1502, 2068, 2273, 2378, 3044
BpuMI CCSGG 2 cut(s) 1354, 3690
BsaBI GATNNNNATC 3 cut(s) 1221, 1665, 3456
BsaI GGTCTC 2 cut(s) 1860, 2873
BsaJI CCNNGG 7 cut(s) 88, 533, 1274, 1299, 1909, 2066, 2305
BsaWI WCCGGW 1 cut(s) 2104
BsaXI ACNNNNNCTCC 8 cut(s) 129, 159, 245, 275, 278, 308, 2450, 2480
Bsc4I CCNNNNNNNGG 8 cut(s) 91, 730, 1524, 1565, 1712, 1915, 2864, 3815
Bse1I ACTGG 5 cut(s) 1162, 1268, 3809, 3825, 3838
Bse3DI GCAATG 2 cut(s) 147, 1765
Bse8I GATNNNNATC 3 cut(s) 1221, 1665, 3456
BseBI CCWGG 7 cut(s) 1275, 1833, 2057, 2067, 2370, 3300, 3575
BseDI CCNNGG 7 cut(s) 88, 533, 1274, 1299, 1909, 2066, 2305
BseJI GATNNNNATC 3 cut(s) 1221, 1665, 3456
BseLI CCNNNNNNNGG 8 cut(s) 91, 730, 1524, 1565, 1712, 1915, 2864, 3815
BseMI GCAATG 2 cut(s) 147, 1765
BseMII CTCAG 4 cut(s) 789, 1475, 2885, 3306
BseNI ACTGG 5 cut(s) 1162, 1268, 3809, 3825, 3838
BseRI GAGGAG 3 cut(s) 182, 497, 987
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 4 cut(s) 730, 1150, 1455, 3348
BseYI CCCAGC 1 cut(s) 1797
BsgI GTGCAG 1 cut(s) 2542
Bsh1236I CGCG 4 cut(s) 90, 316, 361, 1260
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 9 cut(s) 40, 81, 352, 1083, 2025, 2065, 2532, 3573, 3688
BshNI GGYRCC 1 cut(s) 633
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 2 cut(s) 657, 3432
BsiHKCI CYCGRG 2 cut(s) 675, 2438
BsiSI CCGG 3 cut(s) 1353, 2105, 3689
BslI CCNNNNNNNGG 8 cut(s) 91, 730, 1524, 1565, 1712, 1915, 2864, 3815
BsmAI GTCTC 8 cut(s) 468, 1824, 1860, 1983, 2044, 2216, 2873, 3046
BsmBI CGTCTC 1 cut(s) 468
BsnI GGCC 9 cut(s) 40, 81, 352, 1083, 2025, 2065, 2532, 3573, 3688
Bso31I GGTCTC 2 cut(s) 1860, 2873
BsoBI CYCGRG 2 cut(s) 675, 2438
Bsp119I TTCGAA 2 cut(s) 2100, 3017
Bsp1286I GDGCHC 3 cut(s) 657, 1518, 3432
Bsp143I GATC 9 cut(s) 433, 763, 1222, 1666, 1777, 2081, 2187, 2731, 3586
Bsp19I CCATGG 1 cut(s) 533
Bsp68I TCGCGA 1 cut(s) 1260
BspANI GGCC 9 cut(s) 40, 81, 352, 1083, 2025, 2065, 2532, 3573, 3688
BspCNI CTCAG 4 cut(s) 790, 1474, 2886, 3307
BspFNI CGCG 4 cut(s) 90, 316, 361, 1260
BspHI TCATGA 2 cut(s) 1858, 3180
BspLI GGNNCC 4 cut(s) 17, 39, 618, 635
BspMAI CTGCAG 1 cut(s) 2710
BspPI GGATC 2 cut(s) 1230, 1674
BspT104I TTCGAA 2 cut(s) 2100, 3017
BspT107I GGYRCC 1 cut(s) 633
BspTNI GGTCTC 2 cut(s) 1860, 2873
BsrDI GCAATG 2 cut(s) 147, 1765
BsrI ACTGG 5 cut(s) 1162, 1268, 3809, 3825, 3838
BssECI CCNNGG 7 cut(s) 88, 533, 1274, 1299, 1909, 2066, 2305
BssMI GATC 9 cut(s) 433, 763, 1222, 1666, 1777, 2081, 2187, 2731, 3586
BssSI CACGAG 2 cut(s) 1821, 3612
BssT1I CCWWGG 2 cut(s) 533, 2305
Bst2BI CACGAG 2 cut(s) 1821, 3612
Bst2UI CCWGG 7 cut(s) 1275, 1833, 2057, 2067, 2370, 3300, 3575
Bst4CI ACNGT 4 cut(s) 1445, 1767, 2675, 3650
Bst6I CTCTTC 3 cut(s) 1052, 3054, 3511
BstAPI GCANNNNNTGC 3 cut(s) 1780, 3739, 3846
BstBI TTCGAA 2 cut(s) 2100, 3017
BstC8I GCNNGC 5 cut(s) 42, 63, 993, 3546, 3851
BstDEI CTNAG 7 cut(s) 338, 798, 1461, 1985, 2894, 2969, 3315
BstDSI CCRYGG 4 cut(s) 88, 533, 1299, 1909
BstEII GGTNACC 1 cut(s) 2956
BstFNI CGCG 4 cut(s) 90, 316, 361, 1260
BstH2I RGCGCY 1 cut(s) 2464
BstHHI GCGC 4 cut(s) 61, 363, 2463, 3733
BstKTI GATC 9 cut(s) 436, 766, 1225, 1669, 1780, 2084, 2190, 2734, 3589
BstMAI GTCTC 8 cut(s) 468, 1824, 1860, 1983, 2044, 2216, 2873, 3046
BstMBI GATC 9 cut(s) 433, 763, 1222, 1666, 1777, 2081, 2187, 2731, 3586
BstMCI CGRYCG 1 cut(s) 82
BstNI CCWGG 7 cut(s) 1275, 1833, 2057, 2067, 2370, 3300, 3575
BstNSI RCATGY 2 cut(s) 1724, 2886
BstPAI GACNNNNGTC 1 cut(s) 1017
BstPI GGTNACC 1 cut(s) 2956
BstSCI CCNGG 9 cut(s) 1273, 1352, 1831, 2055, 2065, 2368, 3298, 3573, 3688
BstSFI CTRYAG 1 cut(s) 2706
BstUI CGCG 4 cut(s) 90, 316, 361, 1260
BstV1I GCAGC 4 cut(s) 730, 1150, 1455, 3348
BstV2I GAAGAC 4 cut(s) 1869, 1982, 2984, 3248
BstX2I RGATCY 1 cut(s) 2081
BstYI RGATCY 1 cut(s) 2081
BstZI CGGCCG 1 cut(s) 79
BsuI GTATCC 1 cut(s) 951
BsuRI GGCC 9 cut(s) 40, 81, 352, 1083, 2025, 2065, 2532, 3573, 3688
BtgI CCRYGG 4 cut(s) 88, 533, 1299, 1909
BtgZI GCGATG 1 cut(s) 331
BtrI CACGTC 1 cut(s) 3335
BtsIMutI CAGTG 3 cut(s) 3119, 3831, 3832
BtuMI TCGCGA 1 cut(s) 1260
Cac8I GCNNGC 5 cut(s) 42, 63, 993, 3546, 3851
CaiI CAGNNNCTG 1 cut(s) 1468
CciI TCATGA 2 cut(s) 1858, 3180
CfoI GCGC 4 cut(s) 61, 363, 2463, 3733
Cfr13I GGNCC 8 cut(s) 15, 38, 350, 721, 1432, 2011, 2753, 3686
Cfr42I CCGCGG 1 cut(s) 91
CseI GACGC 1 cut(s) 3178
Csp6I GTAC 8 cut(s) 142, 262, 325, 919, 944, 3114, 3419, 3630
CviQI GTAC 8 cut(s) 142, 262, 325, 919, 944, 3114, 3419, 3630
DdeI CTNAG 7 cut(s) 338, 798, 1461, 1985, 2894, 2969, 3315
DpnI GATC 9 cut(s) 435, 765, 1224, 1668, 1779, 2083, 2189, 2733, 3588
DpnII GATC 9 cut(s) 433, 763, 1222, 1666, 1777, 2081, 2187, 2731, 3586
DraI TTTAAA 5 cut(s) 1069, 1392, 2176, 2793, 3676
DraIII CACNNNGTG 1 cut(s) 1328
EaeI YGGCCR 1 cut(s) 79
EagI CGGCCG 1 cut(s) 79
Eam1104I CTCTTC 3 cut(s) 1052, 3054, 3511
EarI CTCTTC 3 cut(s) 1052, 3054, 3511
EciI GGCGGA 2 cut(s) 179, 266
Ecl136II GAGCTC 1 cut(s) 655
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 2 cut(s) 533, 2305
Eco147I AGGCCT 1 cut(s) 2025
Eco24I GRGCYC 2 cut(s) 657, 1518
Eco31I GGTCTC 2 cut(s) 1860, 2873
Eco47I GGWCC 5 cut(s) 15, 721, 1432, 2011, 2753
Eco52I CGGCCG 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 655
Eco57I CTGAAG 5 cut(s) 1568, 2982, 3260, 3524, 3716
Eco88I CYCGRG 2 cut(s) 675, 2438
Eco91I GGTNACC 1 cut(s) 2956
EcoICRI GAGCTC 1 cut(s) 655
EcoO109I RGGNCCY 2 cut(s) 15, 38
EcoO65I GGTNACC 1 cut(s) 2956
EcoRII CCWGG 7 cut(s) 1273, 1831, 2055, 2065, 2368, 3298, 3573
EcoT14I CCWWGG 2 cut(s) 533, 2305
EcoT22I ATGCAT 4 cut(s) 572, 1776, 2633, 3142
EcoT38I GRGCYC 2 cut(s) 657, 1518
ErhI CCWWGG 2 cut(s) 533, 2305
Esp3I CGTCTC 1 cut(s) 468
FauI CCCGC 1 cut(s) 83
FriOI GRGCYC 2 cut(s) 657, 1518
FspBI CTAG 5 cut(s) 1085, 1604, 1685, 3092, 3771
FspI TGCGCA 1 cut(s) 3732
GlaI GCGC 4 cut(s) 60, 362, 2462, 3732
GsaI CCCAGC 1 cut(s) 1801
GsuI CTGGAG 2 cut(s) 1179, 2211
HaeII RGCGCY 1 cut(s) 2464
HaeIII GGCC 9 cut(s) 40, 81, 352, 1083, 2025, 2065, 2532, 3573, 3688
HapII CCGG 3 cut(s) 1353, 2105, 3689
HgaI GACGC 1 cut(s) 3178
HhaI GCGC 4 cut(s) 61, 363, 2463, 3733
Hin6I GCGC 4 cut(s) 59, 361, 2461, 3731
HinP1I GCGC 4 cut(s) 59, 361, 2461, 3731
HincII GTYRAC 2 cut(s) 868, 1379
HindII GTYRAC 2 cut(s) 868, 1379
HindIII AAGCTT 3 cut(s) 999, 2321, 3020
HpaII CCGG 3 cut(s) 1353, 2105, 3689
HphI GGTGA 9 cut(s) 86, 217, 504, 1379, 1973, 2924, 2968, 3581, 3712
Hpy166II GTNNAC 6 cut(s) 262, 868, 1379, 2011, 2671, 2851
Hpy8I GTNNAC 6 cut(s) 262, 868, 1379, 2011, 2671, 2851
Hpy99I CGWCG 3 cut(s) 58, 1815, 3336
HpyCH4III ACNGT 4 cut(s) 1445, 1767, 2675, 3650
HpyCH4IV ACGT 4 cut(s) 144, 1840, 3116, 3334
HpyF3I CTNAG 7 cut(s) 338, 798, 1461, 1985, 2894, 2969, 3315
HpySE526I ACGT 4 cut(s) 144, 1840, 3116, 3334
HspAI GCGC 4 cut(s) 59, 361, 2461, 3731
KspI CCGCGG 1 cut(s) 91
Kzo9I GATC 9 cut(s) 433, 763, 1222, 1666, 1777, 2081, 2187, 2731, 3586
LmnI GCTCC 6 cut(s) 616, 1160, 1278, 1415, 2458, 2918
Lsp1109I GCAGC 4 cut(s) 730, 1150, 1455, 3348
LweI GCATC 8 cut(s) 957, 1028, 1360, 2618, 2759, 3127, 3149, 3203
MaeI CTAG 5 cut(s) 1085, 1604, 1685, 3092, 3771
MaeII ACGT 4 cut(s) 144, 1840, 3116, 3334
MaeIII GTNAC 8 cut(s) 74, 1439, 1672, 1694, 2132, 2939, 2956, 3117
MalI GATC 9 cut(s) 435, 765, 1224, 1668, 1779, 2083, 2189, 2733, 3588
MboI GATC 9 cut(s) 433, 763, 1222, 1666, 1777, 2081, 2187, 2731, 3586
MfeI CAATTG 4 cut(s) 477, 819, 1536, 2514
MflI RGATCY 1 cut(s) 2081
MhlI GDGCHC 3 cut(s) 657, 1518, 3432
MlyI GAGTC 7 cut(s) 494, 600, 1826, 2046, 2218, 3590, 3723
MmeI TCCRAC 2 cut(s) 915, 1860
Mph1103I ATGCAT 4 cut(s) 572, 1776, 2633, 3142
MroXI GAANNNNTTC 2 cut(s) 1806, 3245
MslI CAYNNNNRTG 3 cut(s) 1121, 1919, 3333
MspA1I CMGCKG 1 cut(s) 90
MspI CCGG 3 cut(s) 1353, 2105, 3689
MspR9I CCNGG 9 cut(s) 1275, 1354, 1833, 2057, 2067, 2370, 3300, 3575, 3690
MunI CAATTG 4 cut(s) 477, 819, 1536, 2514
MvaI CCWGG 7 cut(s) 1275, 1833, 2057, 2067, 2370, 3300, 3575
MvnI CGCG 4 cut(s) 90, 316, 361, 1260
NciI CCSGG 2 cut(s) 1354, 3690
NcoI CCATGG 1 cut(s) 533
NdeII GATC 9 cut(s) 433, 763, 1222, 1666, 1777, 2081, 2187, 2731, 3586
NlaIV GGNNCC 4 cut(s) 17, 39, 618, 635
NmeAIII GCCGAG 1 cut(s) 324
NmuCI GTSAC 7 cut(s) 74, 1439, 1672, 1694, 2132, 2956, 3117
NruI TCGCGA 1 cut(s) 1260
NsbI TGCGCA 1 cut(s) 3732
NsiI ATGCAT 4 cut(s) 572, 1776, 2633, 3142
NspI RCATGY 2 cut(s) 1724, 2886
NspV TTCGAA 2 cut(s) 2100, 3017
PagI TCATGA 2 cut(s) 1858, 3180
PceI AGGCCT 1 cut(s) 2025
PciI ACATGT 2 cut(s) 1720, 2882
PcsI WCGNNNNNNNCGW 1 cut(s) 1819
PdmI GAANNNNTTC 2 cut(s) 1806, 3245
PflMI CCANNNNNTGG 1 cut(s) 1524
PfoI TCCNGGA 2 cut(s) 2055, 3298
PleI GAGTC 7 cut(s) 493, 599, 1825, 2046, 2218, 3589, 3722
PpsI GAGTC 7 cut(s) 493, 599, 1825, 2046, 2218, 3589, 3722
PpuMI RGGWCCY 1 cut(s) 15
PscI ACATGT 2 cut(s) 1720, 2882
PshAI GACNNNNGTC 1 cut(s) 1017
PshBI ATTAAT 1 cut(s) 2402
Psp124BI GAGCTC 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 15
Psp6I CCWGG 7 cut(s) 1273, 1831, 2055, 2065, 2368, 3298, 3573
PspEI GGTNACC 1 cut(s) 2956
PspFI CCCAGC 1 cut(s) 1797
PspGI CCWGG 7 cut(s) 1273, 1831, 2055, 2065, 2368, 3298, 3573
PspN4I GGNNCC 4 cut(s) 17, 39, 618, 635
PspPI GGNCC 8 cut(s) 15, 38, 350, 721, 1432, 2011, 2753, 3686
PspPPI RGGWCCY 1 cut(s) 15
PstI CTGCAG 1 cut(s) 2710
PstNI CAGNNNCTG 1 cut(s) 1468
PsuI RGATCY 1 cut(s) 2081
RruI TCGCGA 1 cut(s) 1260
RsaI GTAC 8 cut(s) 143, 263, 326, 920, 945, 3115, 3420, 3631
RsaNI GTAC 8 cut(s) 142, 262, 325, 919, 944, 3114, 3419, 3630
RseI CAYNNNNRTG 3 cut(s) 1121, 1919, 3333
SacI GAGCTC 1 cut(s) 657
SacII CCGCGG 1 cut(s) 91
Sau3AI GATC 9 cut(s) 433, 763, 1222, 1666, 1777, 2081, 2187, 2731, 3586
Sau96I GGNCC 8 cut(s) 15, 38, 350, 721, 1432, 2011, 2753, 3686
SchI GAGTC 7 cut(s) 494, 600, 1826, 2046, 2218, 3590, 3723
ScrFI CCNGG 9 cut(s) 1275, 1354, 1833, 2057, 2067, 2370, 3300, 3575, 3690
SduI GDGCHC 3 cut(s) 657, 1518, 3432
SfaNI GCATC 8 cut(s) 957, 1028, 1360, 2618, 2759, 3127, 3149, 3203
SfcI CTRYAG 1 cut(s) 2706
Sfr303I CCGCGG 1 cut(s) 91
SfuI TTCGAA 2 cut(s) 2100, 3017
SgrBI CCGCGG 1 cut(s) 91
SinI GGWCC 5 cut(s) 15, 721, 1432, 2011, 2753
SmiMI CAYNNNNRTG 3 cut(s) 1121, 1919, 3333
SmlI CTYRAG 7 cut(s) 996, 1384, 1481, 2047, 2252, 2357, 3023
SmoI CTYRAG 7 cut(s) 996, 1384, 1481, 2047, 2252, 2357, 3023
SseBI AGGCCT 1 cut(s) 2025
SspI AATATT 3 cut(s) 1626, 2143, 3310
SspMI CTAG 5 cut(s) 1085, 1604, 1685, 3092, 3771
SstI GAGCTC 1 cut(s) 657
StuI AGGCCT 1 cut(s) 2025
StyD4I CCNGG 9 cut(s) 1273, 1352, 1831, 2055, 2065, 2368, 3298, 3573, 3688
StyI CCWWGG 2 cut(s) 533, 2305
TaaI ACNGT 4 cut(s) 1445, 1767, 2675, 3650
TaiI ACGT 4 cut(s) 147, 1843, 3119, 3337
TaqI TCGA 5 cut(s) 1813, 2100, 3017, 3286, 3331
TatI WGTACW 2 cut(s) 918, 3418
TscAI CASTG 3 cut(s) 3126, 3832, 3838
TseFI GTSAC 7 cut(s) 74, 1439, 1672, 1694, 2132, 2956, 3117
TseI GCWGC 4 cut(s) 743, 1163, 1468, 3361
Tsp45I GTSAC 7 cut(s) 74, 1439, 1672, 1694, 2132, 2956, 3117
TspGWI ACGGA 8 cut(s) 884, 1316, 1898, 2064, 3225, 3647, 3707, 3768
TspRI CASTG 3 cut(s) 3126, 3832, 3838
Van91I CCANNNNNTGG 1 cut(s) 1524
VpaK11BI GGWCC 5 cut(s) 15, 721, 1432, 2011, 2753
VspI ATTAAT 1 cut(s) 2402
XapI RAATTY 7 cut(s) 787, 1130, 1360, 1972, 2170, 2933, 3145
XbaI TCTAGA 1 cut(s) 1603
XceI RCATGY 2 cut(s) 1724, 2886
XcmI CCANNNNNNNNNTGG 3 cut(s) 1120, 2303, 3335
XmnI GAANNNNTTC 2 cut(s) 1806, 3245
XspI CTAG 5 cut(s) 1085, 1604, 1685, 3092, 3771
Zsp2I ATGCAT 4 cut(s) 572, 1776, 2633, 3142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.