Rmu_sc0000745.1_g000041

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000745.1
OG0000105
Physical Location & Seq
Reverse (-)
169787 .. 174031
4245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000745.1_g000041.1.cds

Sequence Viewer

Length: 3546 bp
atggcgtcaaccgcttctcgccgtcgatggacgtacgatgtgtttctaagtttcagagggccagatacgcgcaaggggattacctccgacctgtttaatagactgcaacggagaggaatcaaaaccttcatggatgatcaggatcttcaggtaggagatgtcattagcccgtctcttttagctgcaattgaggagtctagatttgccattgtcattctctcacagaactacgctaattcgtcatggtgcttggaagaactcgtcaagattattcaatgcatgaaagagaaggggcaacgagtcatgcccttgttctactccgtggagccttctgatgtgcggcaccaaaggggaagttttgagctcaaaaggaaaccccaagtagatgtggaagtaagggaacatgaagaagtttatggcaagaatgaggacaaattaaaggcgtggagagctgctttgacagaggtagccaatctttctggaactcatatcgaacttgttgacgcaattgttgggacgattgagggtaaggtggtgccaaaatcctcaagtggtgacaagggctttgtaggaatggatgtccgtgttgatgttttattacataagtacatacatcaagagttggatgaggtacgaattatagggatacttgggatgcgaggagttggtaagacaacgcttgctcgagctttttatgatgacattggtcaggattatgagcatagatgctttctcttcaatgttagagaaaagtttaaaagggttgatggcctagtttctttacaaaagaaccttctttccagcattttgatggacaaatttgaatacatagaggataaatatactggagctgctatgattaaaagaagtttatgtaaaaaaaaggtgctaattgttattgatgatgtggatcagttggcacaattacgaaacttggctggaagtcgcgactggttttgtccagggagcagaattatcataaccaccacggataatcacttgttagtggcacatggtgttgatgatacatacaaggctaccgggttaaattctggtgatgctcgtcaactcttggagttaaaggctttcaagaaaagctccccaccagaaaattatttggacctgtgtgaccgtattttagggtatgctcaggggctgccgttagctcttgaggttttaggttcttttttatgtggtagagagcccaatgaatgggaaagtgcaattgataggctaaaaaatagaccagatagcggaattatggatgtgcttcagattagttttgatggtctagaggacaaagagagagaaatattcctacatattgcttgtttttacaaggggcaagatagggatcgtgtgacacaaatactagactattgtgacctgaaccctgtcattggattacgtgttcttgctgataaatctctcattactacttccagcaatgaactgtcaatgcatgattttctacaagaaatgggctgtgaaatagttcgtcgagagtctcctaaagaggcaggcaaacgtagtagattatggtctcatgaagacatccacaaaatgttgaagaaaaataagggaacaggagcaatccaagggatggtaatggagttacctaaattagaagtggctcattggaatccagaagccttctcaaatttgtctcaacttaatcttctccatattcgtaatgtggaccttcccgaaggcctcacttgtctttctgattccttgagactcctggaatggcctgggtgtccgttaagatctctcccacaaaagttcgaatcagatgaacttattgaacttaacttgtgtcacagcaatattgaacatctttgggtgggaacaaagaattttgagaagttgaagttcatcaaactctgccattctctaaacattgtggagaccccagacctcgcgggcgttcagaatcttgagagtttagatcttgaaggatgtaagagtttggtaagaatccatcaatccctcggatttctcaaaaagcttattgtcctgaatcttaaagactgcaaaagtcttgagaatctgccaggtagaattgaaatggaatctcttcaaacattaattctgtctaactgctcaaaaattaaaaagattcccgagtttgatggaaatatggagcgtctgtctgtgcttgatcttgatgagactgccattgaggaactgcctgtttcaattggacgcctgagtggccttgtgtcattgaatctaagtaactgcacaaatcttgtccgtcttccaagcaccatcaataatttgaagtttgttaagaatcttaatctttctggatgcatgaaacttggcaaacatcaggaaagcgtgggggagagggaatattatgaggaaaatgatgtgaatagtgggtctgcaatagaaatgctcataaagtatgtgagaggttcaatcgttcgtggacgcgaagtagttttgggatctttacgcttgccttctggattggtgaaaaaagtgaatacaaagccaatgagtttccacttgcctatatcgggtctctgtaatttaacatatctgaacctgagtaattgcaatcttggtgaaggagcatttcccaacgaatttggttacttttcctctttggtgaccttgaatctaagtggaaacaattttgttcgtcttccttcgggcattggattgctttctaagcttgagaactttaacttggagaattgcaagagacttgaagagttgtcagaccttccatcaaatagtagcctagatttaagggcagatggttgtacttcactgaaatacttgtttgttgcatcaaacttgaacagattaaacaaatcatattttaatttcatcaattgcgtcaatataaatggcaatcaaggatgcaataacatagcatttgaaatgctgaagacattcatgtatcagggaatctctgataagagggaaacttttcaaattgtaattcctggaagtaatattcctgagtggttcagccatcgaagtgttgggtgttcattaagtgtggggctgcctccagattgggatagccgtaggtttatgggatttgctgtgtgtgttgtttttgtactccatgagcaccatcaggttgatgagcttgatattcatcaattcaagaattttaacgctactcatcatcttgtatgttgcctgaagctcaatggaagagaattagaagtatacggcagacagcctgcatttcgctttagtgaagagttttgccagctgaactcagatcacctgtggctattctatgtatctcatgataaatactttggtacagagtggtggcataacagttgcagtcagcttgatttcttatttgaaaccagagggccaggtctgaaggtgaaagagtgtggagtccgtctgatatatgagcaagatgtgcaagagttgaaccaaacaacaactcaatcaagcagtaggatgtctccatatgaggatatattggttgattttgatattccagttgaaggggaaaccagtggcccttgtagcagaacttgcagacttgaagaattgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1181

Amino Acids

133.9

Weight (kDa)

5.99

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 342, 535
AccB7I CCANNNNNTGG 2 cut(s) 1212, 1603
AccI GTMKAC 1 cut(s) 3198
AccII CGCG 4 cut(s) 70, 948, 1925, 2445
AciI CCGC 4 cut(s) 12, 340, 1254, 1925
AclWI GGATC 4 cut(s) 150, 918, 1362, 2467
AcsI RAATTY 6 cut(s) 818, 1048, 1660, 1858, 2600, 3136
AcuI CTGAAG 5 cut(s) 131, 1256, 2927, 3191, 3383
AcyI GRCGYC 2 cut(s) 5, 2209
AdeI CACNNNGTG 1 cut(s) 1016
AfaI GTAC 6 cut(s) 35, 608, 633, 2782, 3087, 3298
AfiI CCNNNNNNNGG 6 cut(s) 1212, 1253, 1400, 1603, 2531, 3494
AflIII ACRYGT 1 cut(s) 1408
AjnI CCWGG 6 cut(s) 961, 1743, 1753, 2056, 2965, 3355
AjuI GAANNNNNNNTTGG 2 cut(s) 2687, 2719
AloI GAACNNNNNNTCC 4 cut(s) 782, 814, 1395, 1427
Alw21I GWGCWC 2 cut(s) 366, 3099
AlwI GGATC 4 cut(s) 150, 918, 1362, 2467
AlwNI CAGNNNCTG 1 cut(s) 1156
Ama87I CYCGRG 2 cut(s) 684, 2126
AoxI GGCC 7 cut(s) 59, 769, 1711, 1751, 2218, 3353, 3508
ApeKI GCWGC 5 cut(s) 182, 452, 851, 1156, 3028
ApoI RAATTY 6 cut(s) 818, 1048, 1660, 1858, 2600, 3136
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
AseI ATTAAT 1 cut(s) 2090
Asp700I GAANNNNTTC 4 cut(s) 1494, 2079, 2912, 2949
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 5 cut(s) 59, 1120, 1699, 3353, 3509
AsuC2I CCSGG 1 cut(s) 1042
AsuHPI GGTGA 7 cut(s) 566, 1067, 2497, 2591, 2635, 3248, 3379
AsuII TTCGAA 1 cut(s) 1788
AvaI CYCGRG 2 cut(s) 684, 2126
AvaII GGWCC 2 cut(s) 1120, 1699
BanI GGYRCC 2 cut(s) 342, 535
BanII GRGCYC 2 cut(s) 366, 1206
BbsI GAAGAC 4 cut(s) 1557, 2255, 2651, 2915
Bbv12I GWGCWC 2 cut(s) 366, 3099
BbvI GCAGC 5 cut(s) 169, 439, 838, 1143, 3015
BceAI ACGGC 4 cut(s) 6, 1144, 3033, 3217
BciT130I CCWGG 6 cut(s) 963, 1745, 1755, 2058, 2967, 3357
BciVI GTATCC 1 cut(s) 639
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 1 cut(s) 1042
BfaI CTAG 5 cut(s) 198, 773, 1292, 1373, 2759
BfuI GTATCC 1 cut(s) 639
BglI GCCNNNNNGGC 1 cut(s) 2217
BglII AGATCT 2 cut(s) 1769, 1951
BisI GCNGC 6 cut(s) 183, 341, 453, 852, 1157, 3029
BlsI GCNGC 6 cut(s) 184, 342, 454, 853, 1158, 3030
Bme1390I CCNGG 7 cut(s) 963, 1042, 1745, 1755, 2058, 2967, 3357
Bme18I GGWCC 2 cut(s) 1120, 1699
BmeT110I CYCGRG 2 cut(s) 684, 2126
BmgT120I GGNCC 5 cut(s) 59, 1120, 1699, 3353, 3509
BmiI GGNNCC 3 cut(s) 327, 344, 537
BmrFI CCNGG 7 cut(s) 963, 1042, 1745, 1755, 2058, 2967, 3357
BmsI GCATC 6 cut(s) 645, 716, 1048, 2306, 2816, 2870
BoxI GACNNNNGTC 1 cut(s) 705
BpiI GAAGAC 4 cut(s) 1557, 2255, 2651, 2915
BpmI CTGGAG 2 cut(s) 867, 3018
Bpu10I CCTNAGC 1 cut(s) 1149
Bpu14I TTCGAA 1 cut(s) 1788
BpuEI CTTGAG 6 cut(s) 532, 1190, 1756, 1961, 2066, 2711
BpuMI CCSGG 1 cut(s) 1042
BsaAI YACGTR 1 cut(s) 1409
BsaBI GATNNNNATC 4 cut(s) 141, 909, 1353, 3123
BsaHI GRCGYC 2 cut(s) 5, 2209
BsaI GGTCTC 3 cut(s) 1548, 1904, 2540
BsaJI CCNNGG 6 cut(s) 321, 962, 987, 1597, 1754, 1993
BsaXI ACNNNNNCTCC 2 cut(s) 2138, 2168
Bsc4I CCNNNNNNNGG 6 cut(s) 1212, 1253, 1400, 1603, 2531, 3494
Bse1I ACTGG 4 cut(s) 850, 956, 3488, 3504
Bse3DI GCAATG 1 cut(s) 1453
Bse8I GATNNNNATC 4 cut(s) 141, 909, 1353, 3123
BseBI CCWGG 6 cut(s) 963, 1745, 1755, 2058, 2967, 3357
BseDI CCNNGG 6 cut(s) 321, 962, 987, 1597, 1754, 1993
BseJI GATNNNNATC 4 cut(s) 141, 909, 1353, 3123
BseLI CCNNNNNNNGG 6 cut(s) 1212, 1253, 1400, 1603, 2531, 3494
BseMI GCAATG 1 cut(s) 1453
BseMII CTCAG 5 cut(s) 1163, 2204, 2552, 2973, 3264
BseNI ACTGG 4 cut(s) 850, 956, 3488, 3504
BseRI GAGGAG 2 cut(s) 206, 675
BseXI GCAGC 5 cut(s) 169, 439, 838, 1143, 3015
BsgI GTGCAG 1 cut(s) 2230
Bsh1236I CGCG 4 cut(s) 70, 948, 1925, 2445
BshFI GGCC 7 cut(s) 61, 771, 1713, 1753, 2220, 3355, 3510
BshNI GGYRCC 2 cut(s) 342, 535
BsiHKAI GWGCWC 2 cut(s) 366, 3099
BsiHKCI CYCGRG 2 cut(s) 684, 2126
BsiSI CCGG 1 cut(s) 1041
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 529
BslI CCNNNNNNNGG 6 cut(s) 1212, 1253, 1400, 1603, 2531, 3494
BsmBI CGTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 529
BsnI GGCC 7 cut(s) 61, 771, 1713, 1753, 2220, 3355, 3510
Bso31I GGTCTC 3 cut(s) 1548, 1904, 2540
BsoBI CYCGRG 2 cut(s) 684, 2126
Bsp119I TTCGAA 1 cut(s) 1788
Bsp1286I GDGCHC 3 cut(s) 366, 1206, 3099
Bsp143I GATC 9 cut(s) 136, 142, 910, 1354, 1769, 1951, 2164, 2459, 3253
Bsp68I TCGCGA 1 cut(s) 948
BspACI CCGC 4 cut(s) 12, 340, 1254, 1925
BspANI GGCC 7 cut(s) 61, 771, 1713, 1753, 2220, 3355, 3510
BspCNI CTCAG 5 cut(s) 1162, 2205, 2553, 2974, 3263
BspFNI CGCG 4 cut(s) 70, 948, 1925, 2445
BspHI TCATGA 2 cut(s) 1546, 3280
BspLI GGNNCC 3 cut(s) 327, 344, 537
BspPI GGATC 4 cut(s) 150, 918, 1362, 2467
BspT104I TTCGAA 1 cut(s) 1788
BspT107I GGYRCC 2 cut(s) 342, 535
BspTNI GGTCTC 3 cut(s) 1548, 1904, 2540
BsrDI GCAATG 1 cut(s) 1453
BsrI ACTGG 4 cut(s) 850, 956, 3488, 3504
BssECI CCNNGG 6 cut(s) 321, 962, 987, 1597, 1754, 1993
BssMI GATC 9 cut(s) 136, 142, 910, 1354, 1769, 1951, 2164, 2459, 3253
BssNAI GTATAC 1 cut(s) 3199
BssNI GRCGYC 2 cut(s) 5, 2209
BssT1I CCWWGG 1 cut(s) 1597
Bst1107I GTATAC 1 cut(s) 3199
Bst2UI CCWGG 6 cut(s) 963, 1745, 1755, 2058, 2967, 3357
Bst4CI ACNGT 3 cut(s) 1133, 1455, 3317
Bst6I CTCTTC 5 cut(s) 740, 2085, 2721, 3178, 3225
BstACI GRCGYC 2 cut(s) 5, 2209
BstAPI GCANNNNNTGC 2 cut(s) 3406, 3525
BstBAI YACGTR 1 cut(s) 1409
BstBI TTCGAA 1 cut(s) 1788
BstC8I GCNNGC 6 cut(s) 681, 1522, 1927, 2471, 3213, 3242
BstDEI CTNAG 9 cut(s) 47, 1149, 2213, 2237, 2561, 2636, 2685, 2982, 3250
BstDSI CCRYGG 2 cut(s) 321, 987
BstEII GGTNACC 1 cut(s) 2623
BstFNI CGCG 4 cut(s) 70, 948, 1925, 2445
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 9 cut(s) 139, 145, 913, 1357, 1772, 1954, 2167, 2462, 3256
BstMBI GATC 9 cut(s) 136, 142, 910, 1354, 1769, 1951, 2164, 2459, 3253
BstMWI GCNNNNNNNGC 8 cut(s) 11, 67, 449, 2217, 2686, 3406, 3516, 3525
BstNI CCWGG 6 cut(s) 963, 1745, 1755, 2058, 2967, 3357
BstPAI GACNNNNGTC 1 cut(s) 705
BstPI GGTNACC 1 cut(s) 2623
BstSCI CCNGG 7 cut(s) 961, 1040, 1743, 1753, 2056, 2965, 3355
BstUI CGCG 4 cut(s) 70, 948, 1925, 2445
BstV1I GCAGC 5 cut(s) 169, 439, 838, 1143, 3015
BstV2I GAAGAC 4 cut(s) 1557, 2255, 2651, 2915
BstX2I RGATCY 4 cut(s) 142, 1769, 1951, 2459
BstYI RGATCY 4 cut(s) 142, 1769, 1951, 2459
BstZ17I GTATAC 1 cut(s) 3199
BsuI GTATCC 1 cut(s) 639
BsuRI GGCC 7 cut(s) 61, 771, 1713, 1753, 2220, 3355, 3510
BtgI CCRYGG 2 cut(s) 321, 987
BtsIMutI CAGTG 2 cut(s) 2786, 3511
BtuMI TCGCGA 1 cut(s) 948
Cac8I GCNNGC 6 cut(s) 681, 1522, 1927, 2471, 3213, 3242
CaiI CAGNNNCTG 1 cut(s) 1156
CciI TCATGA 2 cut(s) 1546, 3280
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 5 cut(s) 59, 1120, 1699, 3353, 3509
CseI GACGC 5 cut(s) 512, 2138, 2217, 2451, 2845
Csp6I GTAC 6 cut(s) 34, 607, 632, 2781, 3086, 3297
CviQI GTAC 6 cut(s) 34, 607, 632, 2781, 3086, 3297
DdeI CTNAG 9 cut(s) 47, 1149, 2213, 2237, 2561, 2636, 2685, 2982, 3250
DpnI GATC 9 cut(s) 138, 144, 912, 1356, 1771, 1953, 2166, 2461, 3255
DpnII GATC 9 cut(s) 136, 142, 910, 1354, 1769, 1951, 2164, 2459, 3253
DraI TTTAAA 1 cut(s) 757
DraIII CACNNNGTG 1 cut(s) 1016
Eam1104I CTCTTC 5 cut(s) 740, 2085, 2721, 3178, 3225
EarI CTCTTC 5 cut(s) 740, 2085, 2721, 3178, 3225
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 1597
Eco147I AGGCCT 1 cut(s) 1713
Eco24I GRGCYC 2 cut(s) 366, 1206
Eco31I GGTCTC 3 cut(s) 1548, 1904, 2540
Eco47I GGWCC 2 cut(s) 1120, 1699
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 5 cut(s) 131, 1256, 2927, 3191, 3383
Eco88I CYCGRG 2 cut(s) 684, 2126
Eco91I GGTNACC 1 cut(s) 2623
EcoICRI GAGCTC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 2623
EcoRII CCWGG 6 cut(s) 961, 1743, 1753, 2056, 2965, 3355
EcoT14I CCWWGG 1 cut(s) 1597
EcoT22I ATGCAT 3 cut(s) 281, 1464, 2321
EcoT38I GRGCYC 2 cut(s) 366, 1206
ErhI CCWWGG 1 cut(s) 1597
Esp3I CGTCTC 1 cut(s) 177
FaqI GGGAC 1 cut(s) 529
FauI CCCGC 1 cut(s) 1918
FauNDI CATATG 1 cut(s) 3457
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 1 cut(s) 3198
Fnu4HI GCNGC 6 cut(s) 183, 341, 453, 852, 1157, 3029
FriOI GRGCYC 2 cut(s) 366, 1206
Fsp4HI GCNGC 6 cut(s) 183, 341, 453, 852, 1157, 3029
FspBI CTAG 5 cut(s) 198, 773, 1292, 1373, 2759
GlaI GCGC 1 cut(s) 71
GluI GCNGC 6 cut(s) 183, 341, 453, 852, 1157, 3029
GsuI CTGGAG 2 cut(s) 867, 3018
HaeIII GGCC 7 cut(s) 61, 771, 1713, 1753, 2220, 3355, 3510
HapII CCGG 1 cut(s) 1041
HgaI GACGC 5 cut(s) 512, 2138, 2217, 2451, 2845
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 2 cut(s) 5, 2209
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 3 cut(s) 9, 502, 1067
HindII GTYRAC 3 cut(s) 9, 502, 1067
HindIII AAGCTT 2 cut(s) 2009, 2687
HpaII CCGG 1 cut(s) 1041
HphI GGTGA 7 cut(s) 566, 1067, 2497, 2591, 2635, 3248, 3379
Hpy166II GTNNAC 6 cut(s) 9, 502, 1067, 1699, 2441, 3199
Hpy8I GTNNAC 6 cut(s) 9, 502, 1067, 1699, 2441, 3199
Hpy99I CGWCG 2 cut(s) 27, 1503
HpyCH4III ACNGT 3 cut(s) 1133, 1455, 3317
HpyCH4IV ACGT 3 cut(s) 32, 1408, 1528
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 67, 449, 2217, 2686, 3406, 3516, 3525
HpyF3I CTNAG 9 cut(s) 47, 1149, 2213, 2237, 2561, 2636, 2685, 2982, 3250
HpySE526I ACGT 3 cut(s) 32, 1408, 1528
Hsp92I GRCGYC 2 cut(s) 5, 2209
HspAI GCGC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 9 cut(s) 136, 142, 910, 1354, 1769, 1951, 2164, 2459, 3253
LmnI GCTCC 7 cut(s) 325, 848, 966, 1103, 1589, 2146, 2585
Lsp1109I GCAGC 5 cut(s) 169, 439, 838, 1143, 3015
LweI GCATC 6 cut(s) 645, 716, 1048, 2306, 2816, 2870
MaeI CTAG 5 cut(s) 198, 773, 1292, 1373, 2759
MaeII ACGT 3 cut(s) 32, 1408, 1528
MaeIII GTNAC 9 cut(s) 554, 1127, 1360, 1382, 1614, 1820, 2240, 2606, 2623
MalI GATC 9 cut(s) 138, 144, 912, 1356, 1771, 1953, 2166, 2461, 3255
MboI GATC 9 cut(s) 136, 142, 910, 1354, 1769, 1951, 2164, 2459, 3253
MfeI CAATTG 5 cut(s) 186, 507, 1224, 2202, 2851
MflI RGATCY 4 cut(s) 142, 1769, 1951, 2459
MhlI GDGCHC 3 cut(s) 366, 1206, 3099
MlyI GAGTC 5 cut(s) 203, 309, 1514, 1734, 3390
MmeI TCCRAC 2 cut(s) 111, 603
Mph1103I ATGCAT 3 cut(s) 281, 1464, 2321
MroXI GAANNNNTTC 4 cut(s) 1494, 2079, 2912, 2949
MslI CAYNNNNRTG 2 cut(s) 809, 3000
MspA1I CMGCKG 1 cut(s) 3244
MspI CCGG 1 cut(s) 1041
MspR9I CCNGG 7 cut(s) 963, 1042, 1745, 1755, 2058, 2967, 3357
MunI CAATTG 5 cut(s) 186, 507, 1224, 2202, 2851
MvaI CCWGG 6 cut(s) 963, 1745, 1755, 2058, 2967, 3357
MvnI CGCG 4 cut(s) 70, 948, 1925, 2445
MwoI GCNNNNNNNGC 8 cut(s) 11, 67, 449, 2217, 2686, 3406, 3516, 3525
NciI CCSGG 1 cut(s) 1042
NdeI CATATG 1 cut(s) 3457
NdeII GATC 9 cut(s) 136, 142, 910, 1354, 1769, 1951, 2164, 2459, 3253
NlaIV GGNNCC 3 cut(s) 327, 344, 537
NmuCI GTSAC 6 cut(s) 554, 1127, 1360, 1382, 1820, 2623
NruI TCGCGA 1 cut(s) 948
NsiI ATGCAT 3 cut(s) 281, 1464, 2321
NspV TTCGAA 1 cut(s) 1788
PaeR7I CTCGAG 1 cut(s) 684
PagI TCATGA 2 cut(s) 1546, 3280
PceI AGGCCT 1 cut(s) 1713
PdmI GAANNNNTTC 4 cut(s) 1494, 2079, 2912, 2949
Pfl23II CGTACG 1 cut(s) 33
PflMI CCANNNNNTGG 2 cut(s) 1212, 1603
PfoI TCCNGGA 2 cut(s) 1743, 2965
PkrI GCNGC 6 cut(s) 184, 342, 454, 853, 1158, 3030
PleI GAGTC 5 cut(s) 202, 308, 1513, 1734, 3389
PpsI GAGTC 5 cut(s) 202, 308, 1513, 1734, 3389
Ppu21I YACGTR 1 cut(s) 1409
PshAI GACNNNNGTC 1 cut(s) 705
PshBI ATTAAT 1 cut(s) 2090
Psp124BI GAGCTC 1 cut(s) 366
Psp6I CCWGG 6 cut(s) 961, 1743, 1753, 2056, 2965, 3355
PspEI GGTNACC 1 cut(s) 2623
PspGI CCWGG 6 cut(s) 961, 1743, 1753, 2056, 2965, 3355
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 3 cut(s) 327, 344, 537
PspPI GGNCC 5 cut(s) 59, 1120, 1699, 3353, 3509
PspXI VCTCGAGB 1 cut(s) 684
PstNI CAGNNNCTG 1 cut(s) 1156
PsuI RGATCY 4 cut(s) 142, 1769, 1951, 2459
PvuII CAGCTG 1 cut(s) 3244
RruI TCGCGA 1 cut(s) 948
RsaI GTAC 6 cut(s) 35, 608, 633, 2782, 3087, 3298
RsaNI GTAC 6 cut(s) 34, 607, 632, 2781, 3086, 3297
RseI CAYNNNNRTG 2 cut(s) 809, 3000
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 6 cut(s) 183, 341, 453, 852, 1157, 3029
Sau3AI GATC 9 cut(s) 136, 142, 910, 1354, 1769, 1951, 2164, 2459, 3253
Sau96I GGNCC 5 cut(s) 59, 1120, 1699, 3353, 3509
SchI GAGTC 5 cut(s) 203, 309, 1514, 1734, 3390
ScrFI CCNGG 7 cut(s) 963, 1042, 1745, 1755, 2058, 2967, 3357
SduI GDGCHC 3 cut(s) 366, 1206, 3099
SfaNI GCATC 6 cut(s) 645, 716, 1048, 2306, 2816, 2870
Sfr274I CTCGAG 1 cut(s) 684
SfuI TTCGAA 1 cut(s) 1788
SinI GGWCC 2 cut(s) 1120, 1699
SlaI CTCGAG 1 cut(s) 684
SmiMI CAYNNNNRTG 2 cut(s) 809, 3000
SmlI CTYRAG 7 cut(s) 547, 684, 1169, 1735, 1940, 2045, 2690
SmoI CTYRAG 7 cut(s) 547, 684, 1169, 1735, 1940, 2045, 2690
SseBI AGGCCT 1 cut(s) 1713
SsiI CCGC 4 cut(s) 12, 340, 1254, 1925
SspI AATATT 4 cut(s) 1314, 1831, 2363, 2977
SspMI CTAG 5 cut(s) 198, 773, 1292, 1373, 2759
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 1713
StyD4I CCNGG 7 cut(s) 961, 1040, 1743, 1753, 2056, 2965, 3355
StyI CCWWGG 1 cut(s) 1597
TaaI ACNGT 3 cut(s) 1133, 1455, 3317
TaiI ACGT 3 cut(s) 35, 1411, 1531
TaqI TCGA 6 cut(s) 25, 492, 685, 1501, 1788, 2998
TatI WGTACW 3 cut(s) 606, 2780, 3085
TauI GCSGC 1 cut(s) 343
TscAI CASTG 2 cut(s) 2793, 3511
TseFI GTSAC 6 cut(s) 554, 1127, 1360, 1382, 1820, 2623
TseI GCWGC 5 cut(s) 182, 452, 851, 1156, 3028
Tsp45I GTSAC 6 cut(s) 554, 1127, 1360, 1382, 1820, 2623
TspGWI ACGGA 7 cut(s) 124, 310, 572, 1004, 1752, 2249, 3374
TspRI CASTG 2 cut(s) 2793, 3511
Van91I CCANNNNNTGG 2 cut(s) 1212, 1603
VpaK11BI GGWCC 2 cut(s) 1120, 1699
VspI ATTAAT 1 cut(s) 2090
XapI RAATTY 6 cut(s) 818, 1048, 1660, 1858, 2600, 3136
XbaI TCTAGA 2 cut(s) 197, 1291
XcmI CCANNNNNNNNNTGG 2 cut(s) 808, 3002
XhoI CTCGAG 1 cut(s) 684
XmiI GTMKAC 1 cut(s) 3198
XmnI GAANNNNTTC 4 cut(s) 1494, 2079, 2912, 2949
XspI CTAG 5 cut(s) 198, 773, 1292, 1373, 2759
Zsp2I ATGCAT 3 cut(s) 281, 1464, 2321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.