Rw1G004800

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Forward (+)
9207073 .. 9210495
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004800.1

Sequence Viewer

Length: 2745 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGGGGGGGAGATACGCGCTATGGTTTTACCCATGATCTGTATACTGCATTAAAGCAGAGAGGAATTCTCACCTTTCGGGATGACCCAGAGCTTGAGAAAGGAAAATCCATTAGACCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATCTGCGATTGTCATTCTCTCGGAGAATTATGCTTGTTCGTCATGGTGCTTGGATGAACTCGTCAAAATTATTCAATGCATGAAAGATATGGGCCAACAAGTCTTCCCCGTCTTCTACGTCGTGGATCCTTCTGATGTGCGGCACCAAACGGGAAGTTTTGAGCTCAAAGGGGAACCCCAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGCCTAGTGAGGACAGACTAAATGCCTGGAGAGCTGCTTTGACTGAGGTCGCCAATCTTTCTGGCTGGCATGCCAAGTATACTCTATATGACTCAAGTTTGACTGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATGTATCTTCGAGTGTTGACAAGGGCTTCATAGGAATGGGTTCCCGCATTGATGTTTTATTAAGCCATTACATATGTCCACAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCAGGGGAGTAGAATTATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTTATGGGTTAAAATGTGATGAAGCATTTCAACTCTTGAGTTTGAAGGCTTTTAGGAAATGTCCCCCACCAGAAGATTATTTGCATCTGTGCTACCATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTGTCGTCGTTTTAGGTTCTTTTCTATTTGGTAGAAGCACTGATGAATGGGCAAGTGCAATAGATAGGTTAAAGAACACACCACATAAACGAATTATGGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAGACGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAGAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCCGACGGCCTCACCTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGAGTATCCCTTGAGATCTCTCCCACAAAATTTTGAAGCAGATGAACTTATTGAACTCAACTTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGACGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAAAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCTTGTAGAATTGAAATGGAATCTCTTGAAACGTTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATCCCCGAGTTTGTTGGAAATATGGAACGTTTGTCGGTGCTTTGTCTTGATCAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGACATTGAATCTGAGCAACTGCAGAAATCTTGTATGTCTTCCAAGTACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGAGATGGAATGTTTCGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATTTCAACTACCCATGATCGCATAAAATATGTGAGAGGTTCAATCTTTCATGGATGCGAAGCAGTTTGGGAATCTTCAAATAAGTTCCTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGCGCCAATGTGTTTCTGCTTGCCTATATCTGGTCTGCGTAATTTAACATATCTGAACTTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGCAACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTCCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGACTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACCTGTTTGATGCATCGAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCATTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

914

Amino Acids

103.24

Weight (kDa)

6.13

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 190 1.6e-49 TIR domain
TIR_2 PF13676 14 - 107 5.1e-12 TIR domain
NB-ARC PF00931 231 - 311 5.2e-07 NB-ARC domain
WHD_ROQ1 PF23282 378 - 449 3e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 547 - 565 2.4e-06 Leucine Rich Repeat
LRR_14 PF23598 578 - 671 8.5e-06 Leucine-rich repeat region
LRR_14 PF23598 619 - 719 8.2e-10 Leucine-rich repeat region
LRR_13 PF23286 638 - 722 3.9e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 3 cut(s) 641, 1441, 2531
AccI GTMKAC 3 cut(s) 95, 494, 2614
AccII CGCG 1 cut(s) 70
AciI CCGC 4 cut(s) 340, 601, 676, 706
AclI AACGTT 2 cut(s) 1925, 1987
AclWI GGATC 7 cut(s) 320, 333, 658, 671, 756, 1200, 1954
AcsI RAATTY 8 cut(s) 117, 1498, 1621, 1696, 1807, 2241, 2459, 2671
AcuI CTGAAG 1 cut(s) 1427
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 2110, 2641
AfiI CCNNNNNNNGG 5 cut(s) 641, 1238, 1441, 2390, 2531
AjnI CCWGG 3 cut(s) 440, 1357, 2524
AjuI GAANNNNNNNTTGG 2 cut(s) 2546, 2578
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 8 cut(s) 1310, 1350, 1386, 1509, 1570, 1742, 1886, 2572
AlwI GGATC 7 cut(s) 320, 333, 658, 671, 756, 1200, 1954
Ama87I CYCGRG 2 cut(s) 1883, 1964
AoxI GGCC 3 cut(s) 292, 1549, 2056
ApeKI GCWGC 3 cut(s) 182, 449, 689
ApoI RAATTY 8 cut(s) 117, 1498, 1621, 1696, 1807, 2241, 2459, 2671
Asp700I GAANNNNTTC 4 cut(s) 595, 900, 1332, 1426
AspLEI GCGC 2 cut(s) 72, 2365
AspS9I GGNCC 2 cut(s) 292, 1537
AsuHPI GGTGA 3 cut(s) 115, 1546, 2450
AvaI CYCGRG 2 cut(s) 1883, 1964
AvaII GGWCC 1 cut(s) 1537
BamHI GGATCC 2 cut(s) 325, 663
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BbsI GAAGAC 6 cut(s) 295, 304, 1395, 1508, 2093, 2510
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 169, 436, 676
BccI CCATC 6 cut(s) 1118, 1830, 2120, 2188, 2611, 2627
BceAI ACGGC 2 cut(s) 1564, 2063
BcgI CGANNNNNNTGC 2 cut(s) 1864, 1898
BciT130I CCWGG 3 cut(s) 442, 1359, 2526
BciVI GTATCC 1 cut(s) 1608
BclI TGATCA 1 cut(s) 2008
BcoDI GTCTC 8 cut(s) 1310, 1350, 1386, 1509, 1570, 1742, 1886, 2572
BfaI CTAG 4 cut(s) 198, 420, 1211, 2618
BfmI CTRYAG 1 cut(s) 2083
BfoI RGCGCY 1 cut(s) 2366
BfuI GTATCC 1 cut(s) 1608
BglII AGATCT 4 cut(s) 200, 1262, 1607, 1789
BisI GCNGC 4 cut(s) 183, 341, 450, 690
BlsI GCNGC 4 cut(s) 184, 342, 451, 691
Bme1390I CCNGG 3 cut(s) 442, 1359, 2526
Bme18I GGWCC 1 cut(s) 1537
BmeT110I CYCGRG 2 cut(s) 1883, 1964
BmgT120I GGNCC 2 cut(s) 292, 1537
BmiI GGNNCC 5 cut(s) 327, 344, 375, 598, 665
BmrFI CCNGG 3 cut(s) 442, 1359, 2526
BmsI GCATC 7 cut(s) 850, 967, 1287, 2144, 2285, 2653, 2675
BoxI GACNNNNGTC 1 cut(s) 461
BpiI GAAGAC 6 cut(s) 295, 304, 1395, 1508, 2093, 2510
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 2 cut(s) 463, 2508
Bpu10I CCTNAGC 1 cut(s) 987
BpuEI CTTGAG 8 cut(s) 167, 493, 931, 1594, 1624, 1799, 2440, 2570
BsaBI GATNNNNATC 2 cut(s) 747, 1191
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1386, 1742
BsaJI CCNNGG 3 cut(s) 667, 1435, 1831
Bsc4I CCNNNNNNNGG 5 cut(s) 641, 1238, 1441, 2390, 2531
Bse1I ACTGG 3 cut(s) 794, 1134, 2059
Bse8I GATNNNNATC 2 cut(s) 747, 1191
BseBI CCWGG 3 cut(s) 442, 1359, 2526
BseDI CCNNGG 3 cut(s) 667, 1435, 1831
BseGI GGATG 6 cut(s) 139, 259, 1138, 1805, 2159, 2300
BseJI GATNNNNATC 2 cut(s) 747, 1191
BseLI CCNNNNNNNGG 5 cut(s) 641, 1238, 1441, 2390, 2531
BseMII CTCAG 5 cut(s) 450, 510, 1001, 1584, 2066
BseNI ACTGG 3 cut(s) 794, 1134, 2059
BseRI GAGGAG 2 cut(s) 206, 1571
BseXI GCAGC 3 cut(s) 169, 436, 676
BseYI CCCAGC 1 cut(s) 1323
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 3 cut(s) 294, 1551, 2058
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 2 cut(s) 1883, 1964
BslFI GGGAC 1 cut(s) 921
BslI CCNNNNNNNGG 5 cut(s) 641, 1238, 1441, 2390, 2531
BsmAI GTCTC 8 cut(s) 1310, 1350, 1386, 1509, 1570, 1742, 1886, 2572
BsmBI CGTCTC 1 cut(s) 1310
BsmFI GGGAC 1 cut(s) 921
BsnI GGCC 3 cut(s) 294, 1551, 2058
Bso31I GGTCTC 2 cut(s) 1386, 1742
BsoBI CYCGRG 2 cut(s) 1883, 1964
Bsp1286I GDGCHC 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 4 cut(s) 340, 601, 676, 706
BspANI GGCC 3 cut(s) 294, 1551, 2058
BspCNI CTCAG 5 cut(s) 451, 511, 1000, 1585, 2067
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 1384
BspLI GGNNCC 5 cut(s) 327, 344, 375, 598, 665
BspMAI CTGCAG 1 cut(s) 2087
BspPI GGATC 7 cut(s) 320, 333, 658, 671, 756, 1200, 1954
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 2 cut(s) 1386, 1742
BsrI ACTGG 3 cut(s) 794, 1134, 2059
BssECI CCNNGG 3 cut(s) 667, 1435, 1831
BssNAI GTATAC 3 cut(s) 96, 495, 2615
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 667, 1435, 1831
Bst1107I GTATAC 3 cut(s) 96, 495, 2615
Bst2UI CCWGG 3 cut(s) 442, 1359, 2526
Bst4CI ACNGT 2 cut(s) 1293, 1344
Bst6I CTCTTC 2 cut(s) 19, 1403
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1306
BstC8I GCNNGC 6 cut(s) 482, 486, 674, 1168, 1223, 2381
BstDEI CTNAG 9 cut(s) 47, 459, 519, 987, 1511, 1593, 2075, 2495, 2544
BstDSI CCRYGG 1 cut(s) 667
BstF5I GGATG 6 cut(s) 139, 259, 1138, 1805, 2159, 2300
BstFNI CGCG 1 cut(s) 70
BstH2I RGCGCY 1 cut(s) 2366
BstHHI GCGC 2 cut(s) 72, 2365
BstMAI GTCTC 8 cut(s) 1310, 1350, 1386, 1509, 1570, 1742, 1886, 2572
BstMWI GCNNNNNNNGC 4 cut(s) 446, 678, 1306, 2545
BstNI CCWGG 3 cut(s) 442, 1359, 2526
BstNSI RCATGY 2 cut(s) 488, 676
BstPAI GACNNNNGTC 1 cut(s) 461
BstSCI CCNGG 3 cut(s) 440, 1357, 2524
BstSFI CTRYAG 1 cut(s) 2083
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 3 cut(s) 169, 436, 676
BstV2I GAAGAC 6 cut(s) 295, 304, 1395, 1508, 2093, 2510
BstX2I RGATCY 7 cut(s) 200, 325, 663, 1262, 1607, 1789, 1959
BstYI RGATCY 7 cut(s) 200, 325, 663, 1262, 1607, 1789, 1959
BstZ17I GTATAC 3 cut(s) 96, 495, 2615
BsuI GTATCC 1 cut(s) 1608
BsuRI GGCC 3 cut(s) 294, 1551, 2058
BtgI CCRYGG 1 cut(s) 667
BtsCI GGATG 6 cut(s) 139, 259, 1138, 1805, 2159, 2300
BtsIMutI CAGTG 2 cut(s) 1041, 2645
Cac8I GCNNGC 6 cut(s) 482, 486, 674, 1168, 1223, 2381
CciI TCATGA 1 cut(s) 1384
CfoI GCGC 2 cut(s) 72, 2365
Cfr13I GGNCC 2 cut(s) 292, 1537
Csp6I GTAC 2 cut(s) 2109, 2640
CviQI GTAC 2 cut(s) 2109, 2640
DdeI CTNAG 9 cut(s) 47, 459, 519, 987, 1511, 1593, 2075, 2495, 2544
Eam1104I CTCTTC 2 cut(s) 19, 1403
EarI CTCTTC 2 cut(s) 19, 1403
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 667, 1435, 1831
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 2 cut(s) 1386, 1742
Eco47I GGWCC 1 cut(s) 1537
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 1427
Eco88I CYCGRG 2 cut(s) 1883, 1964
EcoICRI GAGCTC 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 3 cut(s) 440, 1357, 2524
EcoT14I CCWWGG 3 cut(s) 667, 1435, 1831
EcoT22I ATGCAT 3 cut(s) 281, 1302, 2668
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 667, 1435, 1831
Esp3I CGTCTC 1 cut(s) 1310
FaqI GGGAC 1 cut(s) 921
FauI CCCGC 2 cut(s) 608, 669
FauNDI CATATG 1 cut(s) 629
FbaI TGATCA 1 cut(s) 2008
FblI GTMKAC 3 cut(s) 95, 494, 2614
Fnu4HI GCNGC 4 cut(s) 183, 341, 450, 690
FokI GGATG 6 cut(s) 146, 266, 1145, 1812, 2166, 2307
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 4 cut(s) 183, 341, 450, 690
FspBI CTAG 4 cut(s) 198, 420, 1211, 2618
GlaI GCGC 2 cut(s) 71, 2364
GluI GCNGC 4 cut(s) 183, 341, 450, 690
GsaI CCCAGC 1 cut(s) 1327
GsuI CTGGAG 2 cut(s) 463, 2508
HaeII RGCGCY 1 cut(s) 2366
HaeIII GGCC 3 cut(s) 294, 1551, 2058
HhaI GCGC 2 cut(s) 72, 2365
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 70, 2363
HinP1I GCGC 2 cut(s) 70, 2363
HincII GTYRAC 4 cut(s) 9, 574, 756, 1455
HindII GTYRAC 4 cut(s) 9, 574, 756, 1455
HindIII AAGCTT 2 cut(s) 1847, 2546
HphI GGTGA 3 cut(s) 115, 1546, 2450
Hpy188I TCNGA 8 cut(s) 223, 334, 1113, 1773, 2013, 2076, 2415, 2596
Hpy99I CGWCG 4 cut(s) 24, 323, 1013, 1550
HpyAV CCTTC 9 cut(s) 339, 913, 1177, 1550, 1678, 1790, 2343, 2435, 2609
HpyCH4III ACNGT 2 cut(s) 1293, 1344
HpyCH4IV ACGT 5 cut(s) 318, 1366, 1767, 1925, 1987
HpyF10VI GCNNNNNNNGC 4 cut(s) 446, 678, 1306, 2545
HpyF3I CTNAG 9 cut(s) 47, 459, 519, 987, 1511, 1593, 2075, 2495, 2544
HpySE526I ACGT 5 cut(s) 318, 1366, 1767, 1925, 1987
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 70, 2363
Ksp22I TGATCA 1 cut(s) 2008
LmnI GCTCC 2 cut(s) 686, 2444
Lsp1109I GCAGC 3 cut(s) 169, 436, 676
LweI GCATC 7 cut(s) 850, 967, 1287, 2144, 2285, 2653, 2675
MaeI CTAG 4 cut(s) 198, 420, 1211, 2618
MaeII ACGT 5 cut(s) 318, 1366, 1767, 1925, 1987
MaeIII GTNAC 3 cut(s) 1198, 2062, 2465
MfeI CAATTG 3 cut(s) 186, 2040, 2710
MflI RGATCY 7 cut(s) 200, 325, 663, 1262, 1607, 1789, 1959
MhlI GDGCHC 1 cut(s) 366
MlyI GAGTC 4 cut(s) 203, 500, 1572, 1897
MmeI TCCRAC 2 cut(s) 1305, 1954
Mph1103I ATGCAT 3 cut(s) 281, 1302, 2668
MroXI GAANNNNTTC 4 cut(s) 595, 900, 1332, 1426
MslI CAYNNNNRTG 2 cut(s) 590, 1445
MspR9I CCNGG 3 cut(s) 442, 1359, 2526
MunI CAATTG 3 cut(s) 186, 2040, 2710
MvaI CCWGG 3 cut(s) 442, 1359, 2526
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 4 cut(s) 446, 678, 1306, 2545
NcoI CCATGG 1 cut(s) 667
NdeI CATATG 1 cut(s) 629
NlaIV GGNNCC 5 cut(s) 327, 344, 375, 598, 665
NmeAIII GCCGAG 1 cut(s) 2145
NmuCI GTSAC 2 cut(s) 1198, 2062
NsiI ATGCAT 3 cut(s) 281, 1302, 2668
NspI RCATGY 2 cut(s) 488, 676
PaeI GCATGC 2 cut(s) 488, 676
PaeR7I CTCGAG 1 cut(s) 1883
PagI TCATGA 1 cut(s) 1384
PdmI GAANNNNTTC 4 cut(s) 595, 900, 1332, 1426
PfeI GAWTC 8 cut(s) 1427, 1774, 1818, 1861, 1913, 2071, 2312, 2491
PflMI CCANNNNNTGG 3 cut(s) 641, 1441, 2531
PkrI GCNGC 4 cut(s) 184, 342, 451, 691
PleI GAGTC 4 cut(s) 202, 500, 1572, 1896
PpsI GAGTC 4 cut(s) 202, 500, 1572, 1896
PshAI GACNNNNGTC 1 cut(s) 461
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 2 cut(s) 1925, 1987
Psp6I CCWGG 3 cut(s) 440, 1357, 2524
PspFI CCCAGC 1 cut(s) 1323
PspGI CCWGG 3 cut(s) 440, 1357, 2524
PspN4I GGNNCC 5 cut(s) 327, 344, 375, 598, 665
PspPI GGNCC 2 cut(s) 292, 1537
PstI CTGCAG 1 cut(s) 2087
PsuI RGATCY 7 cut(s) 200, 325, 663, 1262, 1607, 1789, 1959
RsaI GTAC 2 cut(s) 2110, 2641
RsaNI GTAC 2 cut(s) 2109, 2640
RseI CAYNNNNRTG 2 cut(s) 590, 1445
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 4 cut(s) 183, 341, 450, 690
Sau96I GGNCC 2 cut(s) 292, 1537
SchI GAGTC 4 cut(s) 203, 500, 1572, 1897
ScrFI CCNGG 3 cut(s) 442, 1359, 2526
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 7 cut(s) 850, 967, 1287, 2144, 2285, 2653, 2675
SfcI CTRYAG 1 cut(s) 2083
Sfr274I CTCGAG 1 cut(s) 1883
SinI GGWCC 1 cut(s) 1537
SlaI CTCGAG 1 cut(s) 1883
SmiMI CAYNNNNRTG 2 cut(s) 590, 1445
SmlI CTYRAG 9 cut(s) 146, 508, 910, 1573, 1603, 1778, 1883, 2419, 2549
SmoI CTYRAG 9 cut(s) 146, 508, 910, 1573, 1603, 1778, 1883, 2419, 2549
SphI GCATGC 2 cut(s) 488, 676
SsiI CCGC 4 cut(s) 340, 601, 676, 706
SspI AATATT 1 cut(s) 1152
SspMI CTAG 4 cut(s) 198, 420, 1211, 2618
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 3 cut(s) 440, 1357, 2524
StyI CCWWGG 3 cut(s) 667, 1435, 1831
TaaI ACNGT 2 cut(s) 1293, 1344
TaiI ACGT 5 cut(s) 321, 1369, 1770, 1928, 1990
TaqI TCGA 6 cut(s) 566, 1295, 1584, 1884, 2205, 2669
TatI WGTACW 1 cut(s) 2639
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 8 cut(s) 1427, 1774, 1818, 1861, 1913, 2071, 2312, 2491
TscAI CASTG 2 cut(s) 1048, 2652
TseFI GTSAC 2 cut(s) 1198, 2062
TseI GCWGC 3 cut(s) 182, 449, 689
Tsp45I GTSAC 2 cut(s) 1198, 2062
TspGWI ACGGA 2 cut(s) 541, 987
TspRI CASTG 2 cut(s) 1048, 2652
Van91I CCANNNNNTGG 3 cut(s) 641, 1441, 2531
VpaK11BI GGWCC 1 cut(s) 1537
XapI RAATTY 8 cut(s) 117, 1498, 1621, 1696, 1807, 2241, 2459, 2671
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 2 cut(s) 488, 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 1829
XhoI CTCGAG 1 cut(s) 1883
XmiI GTMKAC 3 cut(s) 95, 494, 2614
XmnI GAANNNNTTC 4 cut(s) 595, 900, 1332, 1426
XspI CTAG 4 cut(s) 198, 420, 1211, 2618
Zsp2I ATGCAT 3 cut(s) 281, 1302, 2668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.