Rmu_sc0003226.1_g000083

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003226.1
OG0000105
Physical Location & Seq
Forward (+)
283986 .. 285002
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003226.1_g000083.1.cds

Sequence Viewer

Length: 1017 bp
atggatgtccgtgttaatgttttattacgtaattacatacatcaagagttggataaggtacgaattatagggatacatgggatgcgaggagttggtaagacagcggttgctcgagctttttatgatgactttggtcaggatttcgagcagagatgctttcttttcaatgttagagaaaagtttaaaagggatggcctagtttctttacaaaagaacctgctttccagcatgttcatggacaaaactgatttcatagaggatgaacataatggagctgctgtgattcgaaggagtttacataacagaaaggtgcttgttgttattgatgatgtggatcggttgtaccaattagaaaaattggctggaaaacccaactggttcggtccagggagcagaattatcataaccaccacagattttcacttgttagaggtacatggtgttgatgctacatacaatgctaccgggttaagttctgatgaagctcttgaactcttgagtttaaaggctttcaagaaaatgcccccaccagaaaattatttggacctgtgctaccatattttagagtatgctcaggggctgccgttagctcttgtggttataggttcttttttatgtggtagaagctccaatattcgggcaagtgcaattgataggctaaaaaatacaccaaatagaggaatcatggatgtgcttcagattagttttgatggactagaggaaaaagacaaagaaatattcctacatattgcttgtttttacaaggggaaagataggaatcgtgtgacacaaatactagactattgtgagctgaaccctgtcattggattaagagttcttgctgataaatctctcattactatttccagcaacgaactgtccatgcatgatttgctacaagaaatgggctgggaaatagttcgtcaagagtctcctaaagagccaggcaaacgtaggagactatggtctcatgaagacatccacaaaatgttgaagaaaaataaggtaatagcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

38.77

Weight (kDa)

8.17

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 225
AciI CCGC 1 cut(s) 104
AclWI GGATC 1 cut(s) 342
AcuI CTGAAG 1 cut(s) 680
AfaI GTAC 3 cut(s) 60, 344, 435
AfiI CCNNNNNNNGG 3 cut(s) 636, 677, 824
AgsI TTSAA 4 cut(s) 166, 491, 514, 994
AjnI CCWGG 2 cut(s) 385, 943
AloI GAACNNNNNNTCC 4 cut(s) 206, 238, 819, 851
AluBI AGCT 7 cut(s) 116, 275, 485, 590, 627, 811, 1013
AluI AGCT 7 cut(s) 116, 275, 485, 590, 627, 811, 1013
Alw26I GTCTC 3 cut(s) 936, 952, 972
AlwI GGATC 1 cut(s) 342
AlwNI CAGNNNCTG 1 cut(s) 580
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 2 cut(s) 275, 580
Asp700I GAANNNNTTC 1 cut(s) 918
AspS9I GGNCC 2 cut(s) 383, 544
AsuC2I CCSGG 1 cut(s) 466
AsuII TTCGAA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 111
AvaII GGWCC 2 cut(s) 383, 544
BbsI GAAGAC 1 cut(s) 981
BbvI GCAGC 2 cut(s) 262, 567
BccI CCATC 2 cut(s) 185, 704
BceAI ACGGC 1 cut(s) 568
BciT130I CCWGG 2 cut(s) 387, 945
BciVI GTATCC 1 cut(s) 66
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 3 cut(s) 936, 952, 972
BfaI CTAG 3 cut(s) 197, 716, 797
BfuAI ACCTGC 1 cut(s) 225
BfuI GTATCC 1 cut(s) 66
BisI GCNGC 2 cut(s) 276, 581
BlsI GCNGC 2 cut(s) 277, 582
Bme1390I CCNGG 3 cut(s) 387, 466, 945
Bme18I GGWCC 2 cut(s) 383, 544
BmeT110I CYCGRG 1 cut(s) 111
BmgT120I GGNCC 2 cut(s) 383, 544
BmrFI CCNGG 3 cut(s) 387, 466, 945
BmsI GCATC 3 cut(s) 72, 143, 436
BoxI GACNNNNGTC 2 cut(s) 132, 964
BpiI GAAGAC 1 cut(s) 981
Bpu10I CCTNAGC 1 cut(s) 573
Bpu14I TTCGAA 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 517
BpuMI CCSGG 1 cut(s) 466
BsaAI YACGTR 1 cut(s) 29
BsaBI GATNNNNATC 2 cut(s) 333, 777
BsaI GGTCTC 1 cut(s) 972
BsaJI CCNNGG 1 cut(s) 386
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 3 cut(s) 636, 677, 824
Bse1I ACTGG 1 cut(s) 380
Bse8I GATNNNNATC 2 cut(s) 333, 777
BseBI CCWGG 2 cut(s) 387, 945
BseDI CCNNGG 1 cut(s) 386
BseGI GGATG 6 cut(s) 10, 87, 196, 265, 694, 978
BseJI GATNNNNATC 2 cut(s) 333, 777
BseLI CCNNNNNNNGG 3 cut(s) 636, 677, 824
BseMII CTCAG 1 cut(s) 587
BseNI ACTGG 1 cut(s) 380
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 262, 567
BseYI CCCAGC 1 cut(s) 909
BshFI GGCC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 1 cut(s) 465
BslI CCNNNNNNNGG 3 cut(s) 636, 677, 824
BsmAI GTCTC 3 cut(s) 936, 952, 972
BsnI GGCC 1 cut(s) 195
Bso31I GGTCTC 1 cut(s) 972
BsoBI CYCGRG 1 cut(s) 111
Bsp119I TTCGAA 1 cut(s) 286
Bsp143I GATC 1 cut(s) 334
BspACI CCGC 1 cut(s) 104
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 1 cut(s) 586
BspHI TCATGA 1 cut(s) 970
BspMI ACCTGC 1 cut(s) 225
BspPI GGATC 1 cut(s) 342
BspT104I TTCGAA 1 cut(s) 286
BspTNI GGTCTC 1 cut(s) 972
BsrI ACTGG 1 cut(s) 380
BssECI CCNNGG 1 cut(s) 386
BssMI GATC 1 cut(s) 334
Bst2UI CCWGG 2 cut(s) 387, 945
Bst4CI ACNGT 1 cut(s) 879
BstAPI GCANNNNNTGC 1 cut(s) 892
BstBAI YACGTR 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 286
BstDEI CTNAG 1 cut(s) 573
BstF5I GGATG 6 cut(s) 10, 87, 196, 265, 694, 978
BstKTI GATC 1 cut(s) 337
BstMAI GTCTC 3 cut(s) 936, 952, 972
BstMBI GATC 1 cut(s) 334
BstMWI GCNNNNNNNGC 1 cut(s) 892
BstNI CCWGG 2 cut(s) 387, 945
BstNSI RCATGY 1 cut(s) 232
BstPAI GACNNNNGTC 2 cut(s) 132, 964
BstSCI CCNGG 3 cut(s) 385, 464, 943
BstSNI TACGTA 1 cut(s) 29
BstV1I GCAGC 2 cut(s) 262, 567
BstV2I GAAGAC 1 cut(s) 981
BsuI GTATCC 1 cut(s) 66
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 6 cut(s) 10, 87, 196, 265, 694, 978
BveI ACCTGC 1 cut(s) 225
CaiI CAGNNNCTG 1 cut(s) 580
CciI TCATGA 1 cut(s) 970
Cfr13I GGNCC 2 cut(s) 383, 544
Csp6I GTAC 3 cut(s) 59, 343, 434
CviAII CATG 8 cut(s) 77, 229, 235, 437, 685, 883, 887, 971
CviQI GTAC 3 cut(s) 59, 343, 434
DdeI CTNAG 1 cut(s) 573
DpnI GATC 1 cut(s) 336
DpnII GATC 1 cut(s) 334
DraI TTTAAA 2 cut(s) 184, 504
Eco105I TACGTA 1 cut(s) 29
Eco31I GGTCTC 1 cut(s) 972
Eco47I GGWCC 2 cut(s) 383, 544
Eco57I CTGAAG 1 cut(s) 680
Eco88I CYCGRG 1 cut(s) 111
EcoRII CCWGG 2 cut(s) 385, 943
EcoT22I ATGCAT 1 cut(s) 888
FaeI CATG 8 cut(s) 80, 232, 238, 440, 688, 886, 890, 974
FatI CATG 8 cut(s) 76, 228, 234, 436, 684, 882, 886, 970
Fnu4HI GCNGC 2 cut(s) 276, 581
FokI GGATG 6 cut(s) 17, 94, 203, 272, 701, 965
Fsp4HI GCNGC 2 cut(s) 276, 581
FspBI CTAG 3 cut(s) 197, 716, 797
GluI GCNGC 2 cut(s) 276, 581
GsaI CCCAGC 1 cut(s) 913
HaeIII GGCC 1 cut(s) 195
HapII CCGG 1 cut(s) 465
Hin1II CATG 8 cut(s) 80, 232, 238, 440, 688, 886, 890, 974
HinfI GANTC 4 cut(s) 283, 681, 778, 929
HpaII CCGG 1 cut(s) 465
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 2 cut(s) 478, 699
Hpy188III TCNNGA 7 cut(s) 44, 137, 488, 496, 514, 926, 971
Hpy8I GTNNAC 1 cut(s) 296
HpyAV CCTTC 1 cut(s) 282
HpyCH4III ACNGT 1 cut(s) 879
HpyCH4IV ACGT 2 cut(s) 28, 952
HpyCH4V TGCA 2 cut(s) 647, 886
HpyF10VI GCNNNNNNNGC 1 cut(s) 892
HpyF3I CTNAG 1 cut(s) 573
HpySE526I ACGT 2 cut(s) 28, 952
Hsp92II CATG 8 cut(s) 80, 232, 238, 440, 688, 886, 890, 974
Kzo9I GATC 1 cut(s) 334
LmnI GCTCC 3 cut(s) 272, 390, 632
Lsp1109I GCAGC 2 cut(s) 262, 567
LweI GCATC 3 cut(s) 72, 143, 436
MaeI CTAG 3 cut(s) 197, 716, 797
MaeII ACGT 2 cut(s) 28, 952
MaeIII GTNAC 1 cut(s) 784
MalI GATC 1 cut(s) 336
MboI GATC 1 cut(s) 334
MboII GAAGA 2 cut(s) 986, 1006
MfeI CAATTG 1 cut(s) 648
MluCI AATT 7 cut(s) 31, 63, 347, 356, 396, 535, 648
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 5 cut(s) 80, 250, 424, 671, 712
Mph1103I ATGCAT 1 cut(s) 888
MroXI GAANNNNTTC 1 cut(s) 918
MseI TTAA 5 cut(s) 15, 183, 470, 503, 830
MslI CAYNNNNRTG 2 cut(s) 233, 689
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 1 cut(s) 465
MspR9I CCNGG 3 cut(s) 387, 466, 945
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 2 cut(s) 387, 945
MwoI GCNNNNNNNGC 1 cut(s) 892
NciI CCSGG 1 cut(s) 466
NdeII GATC 1 cut(s) 334
NlaIII CATG 8 cut(s) 80, 232, 238, 440, 688, 886, 890, 974
NmuCI GTSAC 1 cut(s) 784
NsiI ATGCAT 1 cut(s) 888
NspI RCATGY 1 cut(s) 232
NspV TTCGAA 1 cut(s) 286
PaeR7I CTCGAG 1 cut(s) 111
PagI TCATGA 1 cut(s) 970
PdmI GAANNNNTTC 1 cut(s) 918
PfeI GAWTC 3 cut(s) 283, 681, 778
PkrI GCNGC 2 cut(s) 277, 582
PleI GAGTC 1 cut(s) 937
PpsI GAGTC 1 cut(s) 937
Ppu21I YACGTR 1 cut(s) 29
PshAI GACNNNNGTC 2 cut(s) 132, 964
Psp6I CCWGG 2 cut(s) 385, 943
PspFI CCCAGC 1 cut(s) 909
PspGI CCWGG 2 cut(s) 385, 943
PspPI GGNCC 2 cut(s) 383, 544
PspXI VCTCGAGB 1 cut(s) 111
PstNI CAGNNNCTG 1 cut(s) 580
RsaI GTAC 3 cut(s) 60, 344, 435
RsaNI GTAC 3 cut(s) 59, 343, 434
RseI CAYNNNNRTG 2 cut(s) 233, 689
SaqAI TTAA 5 cut(s) 15, 183, 470, 503, 830
SatI GCNGC 2 cut(s) 276, 581
Sau3AI GATC 1 cut(s) 334
Sau96I GGNCC 2 cut(s) 383, 544
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 3 cut(s) 387, 466, 945
SfaNI GCATC 3 cut(s) 72, 143, 436
Sfr274I CTCGAG 1 cut(s) 111
SfuI TTCGAA 1 cut(s) 286
SinI GGWCC 2 cut(s) 383, 544
SlaI CTCGAG 1 cut(s) 111
SmiMI CAYNNNNRTG 2 cut(s) 233, 689
SmlI CTYRAG 2 cut(s) 111, 496
SmoI CTYRAG 2 cut(s) 111, 496
SnaBI TACGTA 1 cut(s) 29
Sse9I AATT 7 cut(s) 31, 63, 347, 356, 396, 535, 648
SsiI CCGC 1 cut(s) 104
SspI AATATT 2 cut(s) 634, 738
SspMI CTAG 3 cut(s) 197, 716, 797
StyD4I CCNGG 3 cut(s) 385, 464, 943
TaaI ACNGT 1 cut(s) 879
TaiI ACGT 2 cut(s) 31, 955
TaqI TCGA 3 cut(s) 112, 144, 286
TaqII GACCGA 1 cut(s) 371
TasI AATT 7 cut(s) 31, 63, 347, 356, 396, 535, 648
TfiI GAWTC 3 cut(s) 283, 681, 778
Tru1I TTAA 5 cut(s) 15, 183, 470, 503, 830
Tru9I TTAA 5 cut(s) 15, 183, 470, 503, 830
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 2 cut(s) 275, 580
Tsp45I GTSAC 1 cut(s) 784
TspDTI ATGAA 5 cut(s) 223, 241, 276, 495, 987
VpaK11BI GGWCC 2 cut(s) 383, 544
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XhoI CTCGAG 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 918
XspI CTAG 3 cut(s) 197, 716, 797
Zsp2I ATGCAT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.