Rh2CG149400

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000105
Physical Location & Seq
Forward (+)
13044036 .. 13050279
6244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG149400.1

Sequence Viewer

Length: 4188 bp
ATGACCAGCCAAACAGAACGTCCTTCTTCTTCCCCTCTTGGATGGACATACGATGTGTTCCTCAGTTTCAGCGGTGTAGATACACGCAAAAAATTTACAGACCATCTTTACACTGCTCTGACGCAGAAAGGGATATTCACATTCAGAGATGAAGAGGAACTTGAGAGGGGGACGGCAATCAATCCAAAGCTCTTGAAAGCCATTGAAGAATCAAGATATGTAATAGTCATTCTTTCAAAAAATTACGCCAGTTCAAGATGGTGTTTGGATGAGCTTGCAAAAGCCGTGGAGTGCATGAAAGCCGTGGGACAAACAATCCTGCCGGTGTTCTACGATGTGGATCCATCTGATGTGCGAAAACAAGCGGGGGATCATTTCGGAAAGGCCTTTCTGAAGCACAGAGAGGACTTTAATCCAGACAGGGTGCAGAGGTGGAGAGATGCTCTTTCTCAAGTAGGCAATCTCTCCGGATGGGATTTGCGAGATGGATATGAATCAAAAGTAATTCGAGATATAGTTGGAAAGATTTTCACTACATTAAATCAGCTGGCAATACCCATATTACCGTCAACTCCTCCTCCTCCTCGATGGAAATACGATGTATATGTCAGTTTCCATGGGGATGGTCCTCACTTGGGTTTTGTTGATCATCTGTATGATGCACTAGATAAGAATGCAGTTGTTACCTTTAGAGATGACAAAATACTTGAGAGAGGATCATCCATTTCTTCAGTAGTTCTTAAGGCTATCGAGGAGTCAAGAGTTGCCCTCATCATTGTTTCAGCAAACTATGCTTCTTCATCGTGGTGCTTGGATGAGCTTTGGAATATTTTTCAATGCAAAAGTATGGAGCGAGATATCATACCAGTCTACTACGAAATGAATATATTGCTATTACGGCAACAAGGGGGAGTTAATAAGATCCTTTTGAGACCGGAAGTAACGGAACATGAAGAAGTTTATTGGGAGAAATGGGACAAACTAAATATGTGGCAACAACAGTGCAGGGATGCTTTGGTACAGCTGTGCTACATAGATGGGTTTGCTCTGGACTCCGAGAATGTGCCAGAAACTATAATAGGTCAAATTTGTGGCGAAGTAAAGAAATCGTATACACCATCTACAAATGTACACAAGGGCATGGTAGGACTACAAAAGGGCATGGTAGGACTGGAATCCCGCATTCATGATTTAGTACGTAATTATGTATGTCCACATTTTCTTAACGTACGTATCATAGGAATCCATGGAATGCGAGGAATAGGTAAGACAACTGTTGCCCGAGCTGTTTATCATCATATTCGTGATGGTTTTGAAAGTAGCTGCTTTCTTTCCAATGTTAGAGAAAGGTCCAAAAAGGATGGTCTAGTCTCTTTACAAGAAGAACTTCTTTCCAGAATGCTGTCTACAGAAATTGGAGTAGAGGATGAGTATGCAGGAACTGCTCTGATAAAAAAATGGTTATGCAGGCGAAGAGTACTTTTTGTCATTGATGATGTGGATGATCTAACACAATTGGAGAATTTGGCTGGATGTCATGACTGGTTCGGCCTGGGGAGTAGAATTATCATAACTACCACAGATGTTCATTTATTGAAGGCATATGATGATGGTGTTGATGGTATATATGAGGCGACTGGATTACTATATGGTGAATCTCTTGAACTTTTATGTTTGAAGGCTTTTAAGAAGAGCCAACCACCTGAAGATCATTTGCACCTGTGCCACCATATTTTGAATTATGCATGTGGGCTGCCATTAGCTCTTGAGGTCATTGGTTCCTTTCTATGTGGTAGAAGCATGGGAGAATGGGTAAGTGCCATCGGTAGGCTAAAGAATAAACCAGAAAGAAGTATTATCGATGTGCTTCAGATAAGTTTTGATGGACTAGGGGAAACAGAAAAGGAAATATTCCTACATATTGCATGCTTTTATAAGGGGAAATCCAGGGATCGTGTAATACAAATACTAGACTACTGTCAGCTTTCCCCTGTTATTGGTTTAAGTGTTCTTGTTGACAAATCTCTCGTTACTATTTCCAACAACAAACTGTTGATGCATGATTTACTACAAGAAATGGGCTGGGAAATAGTTCGCCGAGAGTCTCCTAATAATCCAGGCAAACGTAGTAGATTATGGTCTCATGAGGACATCCACACTGTGCTGAAGAAAGATAAGGGAACAGAAGCAGTTCAAGGCATGGTGATGGACTTGCCCAAGTTAGAAGTGGCTCAGTGGAAACCGGAAGCCTTTTCAAATTTGTCTCAACTTAGTTTCCTCCAAATTCGAAATGTGGACCTTCCCAAAGGCCCCACTTGTCTCTCTAATTCGTTGAAACTCCTCGAATGGAGTAGATTCCCTTTAAGGTTTCTCCCACAAGATTTTGAATCAGATGAACTCATTGAACTTAACTTGTGCTACAGCAACATTGAACAGCTTTGGCAAGGAGCAAAGAACTTTGACAAGTTGAAGTTCCTCAAACTCTGCCATTCTGAAAGCCTTTGTGAGACCCCAGACTTCACAGGCGTTCAAAATCTTGAACGATTAGATCTTGAAGGATGTAAAAAGTTGGTTAGAATTCATCCATCCCTTGGAGTTTTCAGAAAGCTTATTCTATTGAATCTTAAAGACTGTGAAAGTCTCATGAGTCTGCCAGATAAAATCGAAATGGAATCTCTCGAAACTCTAATTCTTTCAAACTGCTCAAGTCTTAAGAAGATCCCCGAGTTTGTTGGATATATGGAACGTTTGTTGGTGCTTAATCTTGACAAGACTGCCATTGAAACACTGCCTCCCTCAATTGAGCGCTTAAGTGGCCTCGTTTCCTTGGATTTAAGCAACTGCAAAAATCTTGTTTCTCTTCCTCACACCATTAATGGATTGAACTCCCTCCAAAATCTAAGTCTTTCTGGATGCTTGAAACTTGGCGAACATCAGGGTAGTGTGAGGGAGACGGGGTATATGGAGGGAAATGGTGCAAGCAGAGCTGCTGTAGCAGAATTGCCATCATGGAAAGATAGTTTAAGCCATTGTTTTGAAGTGGTTCGAGAGCATTTCTCATGGTCCTTGCCTTCTAGATCAATGCAAAGAGTCAATACAGAACCAATGAGTATGCAGTTGAATTTATCTGGTCTCTGTAATTTAACACATCTAAACATAAGCAATTGCAATCTCGGTGATGGAGCATTTCCCAACAACTTTGGCTGCTTTCCCTCATTGATGGCATTGAATCTGAGTGGAAATGATTTTGTTAAGCTTCCTTCAAGCATCAAATCATTTCTTAAGCTTGAAAACCTTAACTTGGAGAATTGCAAAAGGCTTAAAGAGTTGTCGGAGCTTCCATCAAATAGTAAATTGGATGTAAGGGCAGATGGCTGTTCATCACTGGAAATGCTGTTTGATGCATCCAATTTCAATAGATTGGAGAAATCATATTTTAATTTCATCAATTGCTTCAGACTAAATGACAGTCAAGGCTGCAGTAACATAGCATTTGAAATGCTGAAGACATTCATTCCTCAGGTAATCTCGAATGCAAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAAAATTCCTGAGTGGTTTGATCATCGAACTGTTGGGAGTTCATTAAGTGTAAAGTTACCTCCATTTTGGAGTAACAGTAAGTTTATGGGGTTTGCTTTTTGTGTTGTTTTTGTAATCCATGAGCTCCATGGGTTTGGTATACCGCATGAATTCAAGACTTTCAACGCTACACATCATCTTGTATGTTGCATGACCCTAAATGGGTTTGAGTTACAACAAGTATATGGCAAACAGCCTGCATTTCGCTTTAGCGAACAGTTTTGCCAGGTCGAGTCAGATCACCTTTGGACATTCTATGTATCCCGTGATAAATATTTTGCTGCAGAATGGTGGAATAGCAGTTGCAGTCAGCTTGAGTTCTTATTTAAAACCAGAGGGCCCGGTCTGAAGGTGAAGGAGTGCGGGGTCCATTTGATATATGAGCAAGGTGTACAAGAGTTGAACCAAAAAATGACTCAATTGAGCAAAAGGGTTTCTCCTTGTGGGAATATATTGATTGATGTTGACAAACCCGTTGAAGAAGAGGAAAAAAGAATAAAAAAAGAAAAGGAGAGTAGAAAAAAGAGAAACCAGTTGAAGCAAAAGCCAGTGAAACTGGTTCCAGAACCTGCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1395

Amino Acids

158.59

Weight (kDa)

6.34

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 17 - 182 2e-52 TIR domain
TIR_2 PF13676 19 - 116 6.1e-18 TIR domain
TIR PF01582 198 - 327 9.1e-27 TIR domain
TIR_2 PF13676 201 - 295 1.4e-10 TIR domain
NB-ARC PF00931 409 - 565 3.2e-17 NB-ARC domain
NACHT PF05729 412 - 508 1.3e-06 NACHT domain
WHD_ROQ1 PF23282 632 - 702 3.5e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 859 - 971 3.4e-09 Leucine-rich repeat region
LRR_13 PF23286 891 - 978 8.7e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1187 - 1334 2.3e-20 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1935
AccB7I CCANNNNNTGG 1 cut(s) 2591
AccI GTMKAC 4 cut(s) 870, 1112, 1406, 3709
AccIII TCCGGA 1 cut(s) 467
AciI CCGC 5 cut(s) 72, 365, 1180, 3713, 3972
AclI AACGTT 1 cut(s) 2746
AclWI GGATC 7 cut(s) 335, 348, 378, 724, 916, 1959, 2713
AcsI RAATTY 9 cut(s) 92, 1086, 1522, 2257, 2283, 2577, 3121, 3573, 3719
AcuI CTGAAG 8 cut(s) 413, 714, 1725, 1853, 2186, 3439, 3524, 3977
AdeI CACNNNGTG 1 cut(s) 2161
AfaI GTAC 6 cut(s) 1020, 1131, 1197, 1230, 1479, 4002
AfeI AGCGCT 1 cut(s) 2807
AfiI CCNNNNNNNGG 6 cut(s) 635, 1827, 1997, 2591, 3301, 3771
AflII CTTAAG 4 cut(s) 740, 2711, 2809, 3281
AjnI CCWGG 5 cut(s) 1551, 1946, 2116, 3562, 3834
Alw21I GWGCWC 1 cut(s) 3696
AlwI GGATC 7 cut(s) 335, 348, 378, 724, 916, 1959, 2713
AlwNI CAGNNNCTG 2 cut(s) 1442, 4178
Ama87I CYCGRG 2 cut(s) 1281, 2723
Aor13HI TCCGGA 1 cut(s) 467
Aor51HI AGCGCT 1 cut(s) 2807
AoxI GGCC 5 cut(s) 384, 1549, 2308, 2815, 3947
ApaI GGGCCC 1 cut(s) 3951
ApeKI GCWGC 6 cut(s) 1323, 1753, 2987, 3204, 3477, 3890
ApoI RAATTY 9 cut(s) 92, 1086, 1522, 2257, 2283, 2577, 3121, 3573, 3719
ArsI GACNNNNNNTTYG 3 cut(s) 36, 2887, 2919
AseI ATTAAT 1 cut(s) 2874
Asp700I GAANNNNTTC 6 cut(s) 1386, 2091, 2190, 3042, 3509, 3546
AspLEI GCGC 1 cut(s) 2808
AspS9I GGNCC 8 cut(s) 626, 1350, 2296, 2309, 3063, 3947, 3948, 3976
AsuC2I CCSGG 1 cut(s) 3951
AsuHPI GGTGA 5 cut(s) 1664, 2215, 3188, 3842, 3973
AsuII TTCGAA 1 cut(s) 2287
AvaI CYCGRG 2 cut(s) 1281, 2723
AvaII GGWCC 5 cut(s) 626, 1350, 2296, 3063, 3976
AxyI CCTNAGG 1 cut(s) 3519
BaeGI GKGCMC 1 cut(s) 3951
BaeI ACNNNNGTAYC 4 cut(s) 1010, 1043, 1216, 1249
BamHI GGATCC 1 cut(s) 340
BanII GRGCYC 2 cut(s) 3696, 3951
BbsI GAAGAC 1 cut(s) 3512
Bbv12I GWGCWC 1 cut(s) 3696
BbvI GCAGC 6 cut(s) 1310, 1740, 2974, 3191, 3464, 3877
BceAI ACGGC 4 cut(s) 189, 269, 287, 914
BciT130I CCWGG 5 cut(s) 1553, 1948, 2118, 3564, 3836
BciVI GTATCC 1 cut(s) 3880
BclI TGATCA 2 cut(s) 646, 3589
BcnI CCSGG 1 cut(s) 3951
BfaI CTAG 6 cut(s) 665, 1367, 1889, 1970, 3075, 4186
BfmI CTRYAG 6 cut(s) 1407, 2419, 2991, 3478, 3891, 4179
BfoI RGCGCY 1 cut(s) 2809
BfrI CTTAAG 4 cut(s) 740, 2711, 2809, 3281
BfuI GTATCC 1 cut(s) 3880
BglII AGATCT 1 cut(s) 2548
BisI GCNGC 6 cut(s) 1324, 1754, 2988, 3205, 3478, 3891
BlsI GCNGC 6 cut(s) 1325, 1755, 2989, 3206, 3479, 3892
BmcAI AGTACT 1 cut(s) 1479
Bme1390I CCNGG 6 cut(s) 1553, 1948, 2118, 3564, 3836, 3951
Bme18I GGWCC 5 cut(s) 626, 1350, 2296, 3063, 3976
BmeT110I CYCGRG 2 cut(s) 1281, 2723
BmgT120I GGNCC 8 cut(s) 626, 1350, 2296, 2309, 3063, 3947, 3948, 3976
BmiI GGNNCC 6 cut(s) 342, 1780, 2311, 3949, 3977, 4170
BmrFI CCNGG 6 cut(s) 1553, 1948, 2118, 3564, 3836, 3951
BmsI GCATC 8 cut(s) 430, 649, 1000, 2046, 2903, 3276, 3391, 3413
BoxI GACNNNNGTC 1 cut(s) 1977
BpiI GAAGAC 1 cut(s) 3512
BplI GAGNNNNNCTC 6 cut(s) 427, 459, 753, 785, 3041, 3073
Bpu14I TTCGAA 1 cut(s) 2287
BpuEI CTTGAG 6 cut(s) 182, 435, 728, 1787, 2689, 3944
BpuMI CCSGG 1 cut(s) 3951
Bsa29I ATCGAT 1 cut(s) 1860
BsaAI YACGTR 2 cut(s) 1199, 1232
BsaBI GATNNNNATC 2 cut(s) 339, 493
BsaI GGTCTC 4 cut(s) 925, 2145, 2501, 3137
BsaJI CCNNGG 9 cut(s) 285, 303, 616, 1246, 1552, 1947, 2590, 2826, 3697
BsaWI WCCGGW 3 cut(s) 467, 934, 2242
Bsc4I CCNNNNNNNGG 6 cut(s) 635, 1827, 1997, 2591, 3301, 3771
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 9 cut(s) 249, 866, 1176, 1547, 1642, 3390, 4141, 4157, 4170
Bse21I CCTNAGG 1 cut(s) 3519
Bse8I GATNNNNATC 2 cut(s) 339, 493
BseAI TCCGGA 1 cut(s) 467
BseBI CCWGG 5 cut(s) 1553, 1948, 2118, 3564, 3836
BseCI ATCGAT 1 cut(s) 1860
BseDI CCNNGG 9 cut(s) 285, 303, 616, 1246, 1552, 1947, 2590, 2826, 3697
BseJI GATNNNNATC 2 cut(s) 339, 493
BseLI CCNNNNNNNGG 6 cut(s) 635, 1827, 1997, 2591, 3301, 3771
BseMII CTCAG 5 cut(s) 76, 2246, 3224, 3533, 3570
BseNI ACTGG 9 cut(s) 249, 866, 1176, 1547, 1642, 3390, 4141, 4157, 4170
BseRI GAGGAG 6 cut(s) 564, 567, 570, 573, 767, 2330
BseSI GKGCMC 1 cut(s) 3951
BseXI GCAGC 6 cut(s) 1310, 1740, 2974, 3191, 3464, 3877
BseYI CCCAGC 1 cut(s) 2082
BsgI GTGCAG 2 cut(s) 446, 1024
BshFI GGCC 5 cut(s) 386, 1551, 2310, 2817, 3949
BshVI ATCGAT 1 cut(s) 1860
BsiHKAI GWGCWC 1 cut(s) 3696
BsiHKCI CYCGRG 2 cut(s) 1281, 2723
BsiSI CCGG 5 cut(s) 323, 468, 935, 2243, 3951
BsiWI CGTACG 1 cut(s) 1228
BslFI GGGAC 3 cut(s) 184, 321, 989
BslI CCNNNNNNNGG 6 cut(s) 635, 1827, 1997, 2591, 3301, 3771
BsmBI CGTCTC 1 cut(s) 2945
BsmFI GGGAC 3 cut(s) 184, 321, 989
BsmI GAATGC 5 cut(s) 679, 1182, 1257, 1404, 3538
BsnI GGCC 5 cut(s) 386, 1551, 2310, 2817, 3949
Bso31I GGTCTC 4 cut(s) 925, 2145, 2501, 3137
BsoBI CYCGRG 2 cut(s) 1281, 2723
Bsp119I TTCGAA 1 cut(s) 2287
Bsp120I GGGCCC 1 cut(s) 3947
Bsp1286I GDGCHC 2 cut(s) 3696, 3951
Bsp13I TCCGGA 1 cut(s) 467
Bsp1407I TGTACA 2 cut(s) 1129, 4000
Bsp19I CCATGG 3 cut(s) 616, 1246, 3697
BspACI CCGC 5 cut(s) 72, 365, 1180, 3713, 3972
BspANI GGCC 5 cut(s) 386, 1551, 2310, 2817, 3949
BspCNI CTCAG 5 cut(s) 75, 2245, 3225, 3532, 3571
BspDI ATCGAT 1 cut(s) 1860
BspEI TCCGGA 1 cut(s) 467
BspHI TCATGA 4 cut(s) 1186, 1537, 2143, 2643
BspLI GGNNCC 6 cut(s) 342, 1780, 2311, 3949, 3977, 4170
BspMAI CTGCAG 3 cut(s) 3482, 3895, 4183
BspPI GGATC 7 cut(s) 335, 348, 378, 724, 916, 1959, 2713
BspQI GCTCTTC 1 cut(s) 1685
BspT104I TTCGAA 1 cut(s) 2287
BspTI CTTAAG 4 cut(s) 740, 2711, 2809, 3281
BspTNI GGTCTC 4 cut(s) 925, 2145, 2501, 3137
BsrFI RCCGGY 1 cut(s) 322
BsrGI TGTACA 2 cut(s) 1129, 4000
BsrI ACTGG 9 cut(s) 249, 866, 1176, 1547, 1642, 3390, 4141, 4157, 4170
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 9 cut(s) 285, 303, 616, 1246, 1552, 1947, 2590, 2826, 3697
BssNAI GTATAC 2 cut(s) 1113, 3710
BssT1I CCWWGG 5 cut(s) 616, 1246, 2590, 2826, 3697
Bst1107I GTATAC 2 cut(s) 1113, 3710
Bst2UI CCWGG 5 cut(s) 1553, 1948, 2118, 3564, 3836
Bst6I CTCTTC 5 cut(s) 147, 1468, 1685, 2865, 4086
BstAFI CTTAAG 4 cut(s) 740, 2711, 2809, 3281
BstAPI GCANNNNNTGC 2 cut(s) 791, 1442
BstAUI TGTACA 2 cut(s) 1129, 4000
BstBAI YACGTR 2 cut(s) 1199, 1232
BstBI TTCGAA 1 cut(s) 2287
BstC8I GCNNGC 6 cut(s) 276, 549, 1469, 1927, 2980, 3807
BstDEI CTNAG 7 cut(s) 62, 2232, 2270, 2900, 3233, 3519, 3579
BstDSI CCRYGG 5 cut(s) 285, 303, 616, 1246, 3697
BstH2I RGCGCY 1 cut(s) 2809
BstHHI GCGC 1 cut(s) 2808
BstMWI GCNNNNNNNGC 6 cut(s) 791, 898, 1442, 2814, 2984, 2993
BstNI CCWGG 5 cut(s) 1553, 1948, 2118, 3564, 3836
BstNSI RCATGY 2 cut(s) 1749, 1929
BstPAI GACNNNNGTC 1 cut(s) 1977
BstSCI CCNGG 6 cut(s) 1551, 1946, 2116, 3562, 3834, 3949
BstSFI CTRYAG 6 cut(s) 1407, 2419, 2991, 3478, 3891, 4179
BstSLI GKGCMC 1 cut(s) 3951
BstSNI TACGTA 2 cut(s) 1199, 1232
BstV1I GCAGC 6 cut(s) 1310, 1740, 2974, 3191, 3464, 3877
BstV2I GAAGAC 1 cut(s) 3512
BstX2I RGATCY 4 cut(s) 340, 921, 2548, 2718
BstXI CCANNNNNNTGG 2 cut(s) 623, 3704
BstYI RGATCY 4 cut(s) 340, 921, 2548, 2718
BstZ17I GTATAC 2 cut(s) 1113, 3710
Bsu15I ATCGAT 1 cut(s) 1860
Bsu36I CCTNAGG 1 cut(s) 3519
BsuI GTATCC 1 cut(s) 3880
BsuRI GGCC 5 cut(s) 386, 1551, 2310, 2817, 3949
BsuTUI ATCGAT 1 cut(s) 1860
BtgI CCRYGG 5 cut(s) 285, 303, 616, 1246, 3697
BtsI GCAGTG 2 cut(s) 111, 2786
BtsIMutI CAGTG 7 cut(s) 111, 1007, 2157, 2240, 2786, 3383, 4164
Cac8I GCNNGC 6 cut(s) 276, 549, 1469, 1927, 2980, 3807
CaiI CAGNNNCTG 2 cut(s) 1442, 4178
CciI TCATGA 4 cut(s) 1186, 1537, 2143, 2643
CfoI GCGC 1 cut(s) 2808
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 8 cut(s) 626, 1350, 2296, 2309, 3063, 3947, 3948, 3976
ClaI ATCGAT 1 cut(s) 1860
CseI GACGC 1 cut(s) 130
Csp6I GTAC 6 cut(s) 1019, 1130, 1196, 1229, 1478, 4001
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
CviQI GTAC 6 cut(s) 1019, 1130, 1196, 1229, 1478, 4001
DdeI CTNAG 7 cut(s) 62, 2232, 2270, 2900, 3233, 3519, 3579
DraI TTTAAA 1 cut(s) 3937
DraIII CACNNNGTG 1 cut(s) 2161
Eam1104I CTCTTC 5 cut(s) 147, 1468, 1685, 2865, 4086
EarI CTCTTC 5 cut(s) 147, 1468, 1685, 2865, 4086
Ecl136II GAGCTC 1 cut(s) 3694
Eco105I TACGTA 2 cut(s) 1199, 1232
Eco130I CCWWGG 5 cut(s) 616, 1246, 2590, 2826, 3697
Eco147I AGGCCT 1 cut(s) 386
Eco24I GRGCYC 2 cut(s) 3696, 3951
Eco31I GGTCTC 4 cut(s) 925, 2145, 2501, 3137
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 5 cut(s) 626, 1350, 2296, 3063, 3976
Eco47III AGCGCT 1 cut(s) 2807
Eco53kI GAGCTC 1 cut(s) 3694
Eco57I CTGAAG 8 cut(s) 413, 714, 1725, 1853, 2186, 3439, 3524, 3977
Eco81I CCTNAGG 1 cut(s) 3519
Eco88I CYCGRG 2 cut(s) 1281, 2723
EcoICRI GAGCTC 1 cut(s) 3694
EcoO109I RGGNCCY 2 cut(s) 2309, 3947
EcoRI GAATTC 2 cut(s) 2577, 3719
EcoRII CCWGG 5 cut(s) 1551, 1946, 2116, 3562, 3834
EcoRV GATATC 1 cut(s) 859
EcoT14I CCWWGG 5 cut(s) 616, 1246, 2590, 2826, 3697
EcoT22I ATGCAT 3 cut(s) 1747, 2061, 3406
EcoT38I GRGCYC 2 cut(s) 3696, 3951
ErhI CCWWGG 5 cut(s) 616, 1246, 2590, 2826, 3697
Esp3I CGTCTC 1 cut(s) 2945
FalI AAGNNNNNCTT 8 cut(s) 144, 176, 1371, 1403, 1374, 1406, 3529, 3561
FaqI GGGAC 3 cut(s) 184, 321, 989
FauI CCCGC 3 cut(s) 358, 1187, 3965
FauNDI CATATG 1 cut(s) 1603
FbaI TGATCA 2 cut(s) 646, 3589
FblI GTMKAC 4 cut(s) 870, 1112, 1406, 3709
Fnu4HI GCNGC 6 cut(s) 1324, 1754, 2988, 3205, 3478, 3891
FriOI GRGCYC 2 cut(s) 3696, 3951
Fsp4HI GCNGC 6 cut(s) 1324, 1754, 2988, 3205, 3478, 3891
FspBI CTAG 6 cut(s) 665, 1367, 1889, 1970, 3075, 4186
GlaI GCGC 1 cut(s) 2807
GluI GCNGC 6 cut(s) 1324, 1754, 2988, 3205, 3478, 3891
GsaI CCCAGC 1 cut(s) 2086
HaeII RGCGCY 1 cut(s) 2809
HaeIII GGCC 5 cut(s) 386, 1551, 2310, 2817, 3949
HapII CCGG 5 cut(s) 323, 468, 935, 2243, 3951
HgaI GACGC 1 cut(s) 130
HhaI GCGC 1 cut(s) 2808
Hin6I GCGC 1 cut(s) 2806
HinP1I GCGC 1 cut(s) 2806
HincII GTYRAC 3 cut(s) 570, 2017, 4075
HindII GTYRAC 3 cut(s) 570, 2017, 4075
HindIII AAGCTT 3 cut(s) 2606, 3254, 3284
HpaII CCGG 5 cut(s) 323, 468, 935, 2243, 3951
HphI GGTGA 5 cut(s) 1664, 2215, 3188, 3842, 3973
HpyAV CCTTC 9 cut(s) 33, 1591, 1672, 2309, 2549, 3081, 3270, 3952, 3958
HpyCH4IV ACGT 6 cut(s) 19, 1198, 1227, 1231, 2125, 2746
HpyF10VI GCNNNNNNNGC 6 cut(s) 791, 898, 1442, 2814, 2984, 2993
HpyF3I CTNAG 7 cut(s) 62, 2232, 2270, 2900, 3233, 3519, 3579
HpySE526I ACGT 6 cut(s) 19, 1198, 1227, 1231, 2125, 2746
HspAI GCGC 1 cut(s) 2806
Kpn2I TCCGGA 1 cut(s) 467
Ksp22I TGATCA 2 cut(s) 646, 3589
LguI GCTCTTC 1 cut(s) 1685
LmnI GCTCC 5 cut(s) 850, 2447, 3182, 3334, 3699
Lsp1109I GCAGC 6 cut(s) 1310, 1740, 2974, 3191, 3464, 3877
LweI GCATC 8 cut(s) 430, 649, 1000, 2046, 2903, 3276, 3391, 3413
MaeI CTAG 6 cut(s) 665, 1367, 1889, 1970, 3075, 4186
MaeII ACGT 6 cut(s) 19, 1198, 1227, 1231, 2125, 2746
MaeIII GTNAC 7 cut(s) 682, 940, 2029, 3482, 3624, 3641, 3780
MfeI CAATTG 5 cut(s) 1514, 2799, 3163, 3448, 4028
MflI RGATCY 4 cut(s) 340, 921, 2548, 2718
MhlI GDGCHC 2 cut(s) 3696, 3951
MlyI GAGTC 7 cut(s) 764, 1046, 2111, 2656, 3099, 3851, 4018
MmeI TCCRAC 4 cut(s) 499, 2064, 2713, 3312
Mph1103I ATGCAT 3 cut(s) 1747, 2061, 3406
MroI TCCGGA 1 cut(s) 467
MroXI GAANNNNTTC 6 cut(s) 1386, 2091, 2190, 3042, 3509, 3546
MslI CAYNNNNRTG 5 cut(s) 621, 654, 805, 1302, 2204
MspA1I CMGCKG 3 cut(s) 72, 547, 1024
MspCI CTTAAG 4 cut(s) 740, 2711, 2809, 3281
MspI CCGG 5 cut(s) 323, 468, 935, 2243, 3951
MspR9I CCNGG 6 cut(s) 1553, 1948, 2118, 3564, 3836, 3951
MunI CAATTG 5 cut(s) 1514, 2799, 3163, 3448, 4028
Mva1269I GAATGC 5 cut(s) 679, 1182, 1257, 1404, 3538
MvaI CCWGG 5 cut(s) 1553, 1948, 2118, 3564, 3836
MwoI GCNNNNNNNGC 6 cut(s) 791, 898, 1442, 2814, 2984, 2993
NciI CCSGG 1 cut(s) 3951
NcoI CCATGG 3 cut(s) 616, 1246, 3697
NdeI CATATG 1 cut(s) 1603
NlaIV GGNNCC 6 cut(s) 342, 1780, 2311, 3949, 3977, 4170
NmeAIII GCCGAG 1 cut(s) 2123
NsiI ATGCAT 3 cut(s) 1747, 2061, 3406
NspI RCATGY 2 cut(s) 1749, 1929
NspV TTCGAA 1 cut(s) 2287
PaeI GCATGC 1 cut(s) 1929
PagI TCATGA 4 cut(s) 1186, 1537, 2143, 2643
PceI AGGCCT 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 1685
PctI GAATGC 5 cut(s) 679, 1182, 1257, 1404, 3538
PdmI GAANNNNTTC 6 cut(s) 1386, 2091, 2190, 3042, 3509, 3546
Pfl23II CGTACG 1 cut(s) 1228
PflMI CCANNNNNTGG 1 cut(s) 2591
PfoI TCCNGGA 1 cut(s) 3562
PkrI GCNGC 6 cut(s) 1325, 1755, 2989, 3206, 3479, 3892
PleI GAGTC 7 cut(s) 763, 1046, 2110, 2655, 3098, 3850, 4018
PpsI GAGTC 7 cut(s) 763, 1046, 2110, 2655, 3098, 3850, 4018
Ppu21I YACGTR 2 cut(s) 1199, 1232
PshAI GACNNNNGTC 1 cut(s) 1977
PshBI ATTAAT 1 cut(s) 2874
PsiI TTATAA 1 cut(s) 1935
Psp124BI GAGCTC 1 cut(s) 3696
Psp1406I AACGTT 1 cut(s) 2746
Psp6I CCWGG 5 cut(s) 1551, 1946, 2116, 3562, 3834
PspFI CCCAGC 1 cut(s) 2082
PspGI CCWGG 5 cut(s) 1551, 1946, 2116, 3562, 3834
PspLI CGTACG 1 cut(s) 1228
PspN4I GGNNCC 6 cut(s) 342, 1780, 2311, 3949, 3977, 4170
PspOMI GGGCCC 1 cut(s) 3947
PspPI GGNCC 8 cut(s) 626, 1350, 2296, 2309, 3063, 3947, 3948, 3976
PstI CTGCAG 3 cut(s) 3482, 3895, 4183
PstNI CAGNNNCTG 2 cut(s) 1442, 4178
PsuI RGATCY 4 cut(s) 340, 921, 2548, 2718
PvuII CAGCTG 2 cut(s) 547, 1024
RsaI GTAC 6 cut(s) 1020, 1131, 1197, 1230, 1479, 4002
RsaNI GTAC 6 cut(s) 1019, 1130, 1196, 1229, 1478, 4001
RseI CAYNNNNRTG 5 cut(s) 621, 654, 805, 1302, 2204
SacI GAGCTC 1 cut(s) 3696
SapI GCTCTTC 1 cut(s) 1685
SatI GCNGC 6 cut(s) 1324, 1754, 2988, 3205, 3478, 3891
Sau96I GGNCC 8 cut(s) 626, 1350, 2296, 2309, 3063, 3947, 3948, 3976
ScaI AGTACT 1 cut(s) 1479
SchI GAGTC 7 cut(s) 764, 1046, 2111, 2656, 3099, 3851, 4018
ScrFI CCNGG 6 cut(s) 1553, 1948, 2118, 3564, 3836, 3951
SduI GDGCHC 2 cut(s) 3696, 3951
SfaNI GCATC 8 cut(s) 430, 649, 1000, 2046, 2903, 3276, 3391, 3413
SfcI CTRYAG 6 cut(s) 1407, 2419, 2991, 3478, 3891, 4179
SfuI TTCGAA 1 cut(s) 2287
SinI GGWCC 5 cut(s) 626, 1350, 2296, 3063, 3976
SmiMI CAYNNNNRTG 5 cut(s) 621, 654, 805, 1302, 2204
SnaBI TACGTA 2 cut(s) 1199, 1232
SphI GCATGC 1 cut(s) 1929
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 5 cut(s) 72, 365, 1180, 3713, 3972
SspI AATATT 3 cut(s) 829, 1911, 3884
SspMI CTAG 6 cut(s) 665, 1367, 1889, 1970, 3075, 4186
SstI GAGCTC 1 cut(s) 3696
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 6 cut(s) 1551, 1946, 2116, 3562, 3834, 3949
StyI CCWWGG 5 cut(s) 616, 1246, 2590, 2826, 3697
TaiI ACGT 6 cut(s) 22, 1201, 1230, 1234, 2128, 2749
TatI WGTACW 3 cut(s) 1129, 1477, 4000
TscAI CASTG 7 cut(s) 118, 1007, 2164, 2240, 2793, 3390, 4164
TseI GCWGC 6 cut(s) 1323, 1753, 2987, 3204, 3477, 3890
TspGWI ACGGA 1 cut(s) 959
TspRI CASTG 7 cut(s) 118, 1007, 2164, 2240, 2793, 3390, 4164
Van91I CCANNNNNTGG 1 cut(s) 2591
Vha464I CTTAAG 4 cut(s) 740, 2711, 2809, 3281
VpaK11BI GGWCC 5 cut(s) 626, 1350, 2296, 3063, 3976
VspI ATTAAT 1 cut(s) 2874
XapI RAATTY 9 cut(s) 92, 1086, 1522, 2257, 2283, 2577, 3121, 3573, 3719
XbaI TCTAGA 1 cut(s) 3074
XceI RCATGY 2 cut(s) 1749, 1929
XcmI CCANNNNNNNNNTGG 2 cut(s) 2224, 3695
XmiI GTMKAC 4 cut(s) 870, 1112, 1406, 3709
XmnI GAANNNNTTC 6 cut(s) 1386, 2091, 2190, 3042, 3509, 3546
XspI CTAG 6 cut(s) 665, 1367, 1889, 1970, 3075, 4186
ZrmI AGTACT 1 cut(s) 1479
Zsp2I ATGCAT 3 cut(s) 1747, 2061, 3406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.