RchiOBHm_Chr6g0251011

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000105
Physical Location & Seq
Forward (+)
6335378 .. 6340486
5109 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22502

Sequence Viewer

Length: 3378 bp
ATGACTACCCAAGCAGATATTCCTTCTTCTTCTGCTCCTCTCCCTAACTACGATGTCTTCCTTAGTTCCAGTGGAGAAGACACCGTTCGAAATTTTACAGATCATCTCTATACTGCTCTGGACCAGAAGGGGATATTTACATTTAGAGATGGTGAAGATGGTGAAGAACTCGTAGCAATTGAAGAATCAAGATGTGCAATTGTGGTTCTTTCAGGAAAGTACACCGATCCTTGGTGTTTGGATGCGATTGCAAAGATCGTTGAATGCAAGGAAGTGATGAAACTGACAGTACTCCCAGTCTTTCATGATGTGGATCCATCTGATGTGCGAAAACAAACAGGGGATCATTTCGGGGAAGCCTTCTCGAAGCATGAAGAGGCCTTTAAACACGAACCGGAGAAGGTGCGCAGGTGGAGACGTGCTCTACTTGAAGTAGCCAATCTCTCTGGATGGCAATTGCAAAATGGGGATGACGGAAAATTGATTGAGGAAATTGTTAGGGCCACAATAAGTAATCTAGGTGATACCTATACCTCTTCAAGTATTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTATTAAGCAATTACATATGTACGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACAGTTGCTCGAGCTATCTATGATGAAATTGGTCAGGATTTTGACCGAAGCTGCTTTCTTTCCAATGTTAGAGAATTGTCTAATCACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCATAATCCTGATGGTAAAAGTTAACAATATAGAGAATGAATACATAGGAGCTTCTATGCTACAAAGGCGGATGTGTAGACTAAAGGTGCTTGTTGTTATTGATGATGTGGATAAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGGGACTGGTTCGGTCCAGGGAGTAGAATCATCATAACCACCACAGATATTCATTTGTTAGAGGCACATGATGTTGATGCTACATACAAGGCTACTGGGTTAACTCGTGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAAATGTACGCCACCAGAAGATTATTTGGACTTGTGCCACTGTATATCATGGTATGCTCAGGGGCTTCCGCTGGCACTTGTGGTTTTAGGTTCTTTTCTATTAGGTAGAAGCGCTGATGAATGGGCAAGTGCAATTGAAAGGCTGAACAATAGACCAAATAGAGTAGTTACGGATGTGCTTCGGATTAGTTTTGATGGACTAGACAATAATGACAAGGGTGTTTTTTTGGACATTGCATGTTTCTTTAAAGGAGAGAGTATAGATCGTGTAACAAGAATACTACACCGATCCAACTGTAATCCAGACATTGTGATACAAGTTCTTGTGGACAGAGCTCTAGTAACTGTGGTTGAAAGACAATTATGGATGCATGACCTGATACAAAAATTGGGTCAAGAAATTGTTTTTGAAGAATGTCCTCAAGAGCCTGGCAATCGTAGCAGGTTGTGGAGTCCGCAAGAAATCATCCATGTACTCGAGATGAATAAGGGTTCAAGTGCACTTCGAGCCATAGCCTTAGACTCTCCAAAAACAGAAGATATACACTGCCACCCAGAGGCATTCTCTGTGATGCAGAGACTTCAGTTTCTCCAAATCCGTAATGTGAATATGAAGGAAGGCCCCAGTTATCTTCCTGGGGGATTAATAGTTCTGGAATGGAGTGGGTATCCTATAGGAAATCTCCCACAAAATTTTGATCCTAGGCAATTATGTGAACTTAACCTCTGCTACAGTAGCATTAAACAACTTTGGAGTGGAATAAAGAACCTGCCCTATTTGACAGTCATCAATTTAAGCTACTCTTGGAAGTTAATTAGGACCCCAGATTTTACACATGCTCCAGGGCTTGAAAGATTAATTCTTGAAGGTTGTACAAGTTTAGTGGACATCCACCCATCCATTGCTCACCTGGAGAGGCTTACTTGCTTGAATCTTAAAGATTGCAGAAGTCTTGAAAGTCTTCCAAGTAAGCTTGAAACAAAATGCCTTCGAATTTTTGTTCTTTCAGGCTGTTCAAAAGTCAAGAAAATTCCAGATTTTGTGGGATGTATGGAAGGCTTGTTAGAACTTTATTTGGATGAGACTGCTATAGAGCAATTACCTATATCAGTTCGACTTCTGACTGGCCTCATTTTATTAAATTTGAGAGATTGCAAAAATCTGAAGAGACTTCCAAGAGACATTGGCAATTTAAAGTCCCTCAAGCGTCTCAATATCTGTGGATGCTCAAAACTTGAAACGCTACCAGACAGCTTGGGAAAGATAGACTGCTTGGAAGAGCTTGATGCGGCTGGTGGTAGTTCCATAAGCAATATACCATCCTCTATTTCCTGTTTGGAAAATCTTGAAGTGTTATCTTTTGGTGGATGTAAAGGGATGTCAGGCAACATGACAAGATATCCCCTCAGTTTTCTGTTGCCCATAAGTGGTTCAGGTGGTTTGCGTTCTTTAACGGAGCTAAATCTCAGTGACTGTAATCTTCAGGAGGGAAGCATACCGGAAGATTTTGGCTGCTTATTCCCTTTAGTTTCACTAAATCTAAGCAAAAACAACTTTGATAGTCTTCCAAAAAGCATCAGACAGCTTTCAAAGCTTCGCAACTTGAATTTGGAAAGTTGTAAGACTCTTCAAAAATTGCCAGACCTCTCATCAAGCTTAAACTTCAGTTTCGGGGCAGACGGCAGTTTTTCACGGGAAAGGCTGTCATCATGTTTCAATCTGATTAATTGCTCCAAATTGGTTGTCAATCAACGATGCAACAATATAGCATTGAAAATGCTATGGAGATCTCTTCAGGGAATTCCTTCTGCAGGAAATAGATTTGAAACTATAATTCCTGGCAGTGAAATCTCTGAGCTGTTCACTGATCAAAGTGCAGGACCAGTTATAAGCATGGAGCTACCTCGACAATGGCAGAAGAATAAGTGGATGGGATATGCATTTTGTGCTTGTTTTGTCATCCGTCGAGATCTTCCAGTTCCAGCTAACCGGCTTGGTAAATGGAAGTTCGGGACCCATAGCACTGCAAATGGTCTTCGATTCGAAGTGAAGCCTAAGAATCTGGGTGTGGCTGGCTGGTGTCCTTCCTTTGTCTGCAGCCAAGAATTAGGCCAAATTGAGTCGAAACACCTTTGGCTATCCTTTGTCTCTGGTGATTATTTTGGAACAGCATGGCAAGAAACCTTTCATAACATCGAGTTTACATTTAAAACATTAGGCACTGGTATGGAGGTGAAGAAGTGTGGAGTCCGTTTGATATACAAGCAAGATTTAGATTTGATTGGAATGACAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1125

Amino Acids

125.87

Weight (kDa)

6.16

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 183 6.4e-39 TIR domain
TIR_2 PF13676 19 - 107 5e-07 TIR domain
NB-ARC PF00931 207 - 361 7.1e-16 NB-ARC domain
WHD_ROQ1 PF23282 429 - 497 1.3e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 679 - 814 2.1e-13 Leucine-rich repeat region
LRR_13 PF23286 690 - 772 1.2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 773 - 909 3.4e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 972 - 1118 7.3e-11 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2969
AarI CACCTGC 1 cut(s) 399
Acc16I TGCGCA 1 cut(s) 407
Acc36I ACCTGC 3 cut(s) 399, 1512, 1857
AccI GTMKAC 1 cut(s) 865
AciI CCGC 6 cut(s) 574, 649, 856, 1148, 1535, 2369
AclWI GGATC 8 cut(s) 221, 308, 321, 351, 631, 644, 1362, 1772
AcsI RAATTY 7 cut(s) 91, 1771, 2073, 2109, 2221, 2686, 2880
AcuI CTGAAG 5 cut(s) 1646, 2264, 2546, 2728, 2858
AfaI GTAC 6 cut(s) 221, 291, 607, 1087, 1554, 1954
AfeI AGCGCT 1 cut(s) 1192
AfiI CCNNNNNNNGG 4 cut(s) 231, 1636, 2507, 2891
AjiI CACGTC 1 cut(s) 419
AjnI CCWGG 6 cut(s) 949, 1507, 1714, 1921, 1989, 2917
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AloI GAACNNNNNNTCC 2 cut(s) 1713, 1745
Alw21I GWGCWC 3 cut(s) 424, 1417, 1582
Alw26I GTCTC 7 cut(s) 409, 1651, 2156, 2242, 2253, 2294, 3232
Alw44I GTGCAC 1 cut(s) 1578
AlwI GGATC 8 cut(s) 221, 308, 321, 351, 631, 644, 1362, 1772
AlwNI CAGNNNCTG 1 cut(s) 2553
Ama87I CYCGRG 2 cut(s) 675, 1556
Aor51HI AGCGCT 1 cut(s) 1192
AoxI GGCC 6 cut(s) 378, 501, 757, 1699, 2206, 3190
ApaLI GTGCAC 1 cut(s) 1578
ApeKI GCWGC 4 cut(s) 610, 717, 2592, 3177
ApoI RAATTY 7 cut(s) 91, 1771, 2073, 2109, 2221, 2686, 2880
ArsI GACNNNNNNTTYG 4 cut(s) 1456, 1488, 2808, 2840
AseI ATTAAT 3 cut(s) 1724, 1937, 2805
Asp700I GAANNNNTTC 6 cut(s) 568, 780, 1050, 2040, 2884, 3264
AspA2I CCTAGG 1 cut(s) 1781
AspLEI GCGC 2 cut(s) 408, 1193
AspS9I GGNCC 7 cut(s) 121, 501, 947, 1700, 1899, 2960, 3093
AsuHPI GGTGA 7 cut(s) 164, 173, 533, 1055, 1979, 3245, 3325
AsuII TTCGAA 3 cut(s) 88, 2071, 3123
AvaI CYCGRG 2 cut(s) 675, 1556
AvaII GGWCC 5 cut(s) 121, 947, 1899, 2960, 3093
AvrII CCTAGG 1 cut(s) 1781
BaeGI GKGCMC 1 cut(s) 1582
BamHI GGATCC 2 cut(s) 313, 636
BanII GRGCYC 1 cut(s) 1417
BauI CACGAG 1 cut(s) 1038
BbsI GAAGAC 5 cut(s) 49, 84, 2033, 2636, 3107
Bbv12I GWGCWC 3 cut(s) 424, 1417, 1582
BbvI GCAGC 4 cut(s) 622, 704, 2579, 3189
BccI CCATC 9 cut(s) 143, 152, 325, 444, 793, 1268, 1984, 2407, 3004
BceAI ACGGC 1 cut(s) 2776
BciT130I CCWGG 6 cut(s) 951, 1509, 1716, 1923, 1991, 2919
BciVI GTATCC 1 cut(s) 1758
BclI TGATCA 1 cut(s) 2947
BcoDI GTCTC 7 cut(s) 409, 1651, 2156, 2242, 2253, 2294, 3232
BfaI CTAG 5 cut(s) 518, 761, 1280, 1418, 1782
BfmI CTRYAG 5 cut(s) 1752, 1810, 2169, 2889, 3175
BfoI RGCGCY 1 cut(s) 1194
BfuAI ACCTGC 3 cut(s) 399, 1512, 1857
BfuI GTATCC 1 cut(s) 1758
BglII AGATCT 2 cut(s) 2867, 3049
BisI GCNGC 5 cut(s) 611, 718, 2370, 2593, 3178
BlnI CCTAGG 1 cut(s) 1781
BlsI GCNGC 5 cut(s) 612, 719, 2371, 2594, 3179
BmcAI AGTACT 1 cut(s) 291
Bme1390I CCNGG 6 cut(s) 951, 1509, 1716, 1923, 1991, 2919
Bme18I GGWCC 5 cut(s) 121, 947, 1899, 2960, 3093
BmeT110I CYCGRG 2 cut(s) 675, 1556
BmgBI CACGTC 1 cut(s) 419
BmgT120I GGNCC 7 cut(s) 121, 501, 947, 1700, 1899, 2960, 3093
BmiI GGNNCC 6 cut(s) 315, 638, 1702, 1901, 3094, 3095
BmrFI CCNGG 6 cut(s) 951, 1509, 1716, 1923, 1991, 2919
BmrI ACTGGG 3 cut(s) 290, 1038, 1698
BmsI GCATC 8 cut(s) 232, 1000, 1437, 1641, 2294, 2356, 2664, 2825
BmuI ACTGGG 3 cut(s) 290, 1038, 1698
BpiI GAAGAC 5 cut(s) 49, 84, 2033, 2636, 3107
BplI GAGNNNNNCTC 4 cut(s) 406, 438, 1628, 1660
BpmI CTGGAG 2 cut(s) 1905, 2012
Bpu10I CCTNAGC 1 cut(s) 1137
Bpu14I TTCGAA 3 cut(s) 88, 2071, 3123
BpuEI CTTGAG 2 cut(s) 1485, 2267
BsaBI GATNNNNATC 1 cut(s) 312
BsaJI CCNNGG 6 cut(s) 230, 640, 950, 1715, 1781, 1922
BsaWI WCCGGW 2 cut(s) 394, 2578
BsaXI ACNNNNNCTCC 2 cut(s) 1903, 1933
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 1636, 2507, 2891
Bse118I RCCGGY 1 cut(s) 3069
Bse1I ACTGG 9 cut(s) 69, 296, 944, 1033, 1704, 2209, 2963, 3056, 3307
Bse3DI GCAATG 2 cut(s) 1311, 1980
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 6 cut(s) 951, 1509, 1716, 1923, 1991, 2919
BseDI CCNNGG 6 cut(s) 230, 640, 950, 1715, 1781, 1922
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 4 cut(s) 231, 1636, 2507, 2891
BseMI GCAATG 2 cut(s) 1311, 1980
BseMII CTCAG 4 cut(s) 1151, 2500, 2560, 2925
BseNI ACTGG 9 cut(s) 69, 296, 944, 1033, 1704, 2209, 2963, 3056, 3307
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 1582
BseXI GCAGC 4 cut(s) 622, 704, 2579, 3189
BsgI GTGCAG 1 cut(s) 2976
BshFI GGCC 6 cut(s) 380, 503, 759, 1701, 2208, 3192
BsiHKAI GWGCWC 3 cut(s) 424, 1417, 1582
BsiHKCI CYCGRG 2 cut(s) 675, 1556
BsiSI CCGG 3 cut(s) 395, 2579, 3070
BslFI GGGAC 3 cut(s) 950, 2263, 3106
BslI CCNNNNNNNGG 4 cut(s) 231, 1636, 2507, 2891
BsmAI GTCTC 7 cut(s) 409, 1651, 2156, 2242, 2253, 2294, 3232
BsmBI CGTCTC 2 cut(s) 409, 2294
BsmFI GGGAC 3 cut(s) 950, 2263, 3106
BsmI GAATGC 2 cut(s) 269, 1640
BsnI GGCC 6 cut(s) 380, 503, 759, 1701, 2208, 3192
BsoBI CYCGRG 2 cut(s) 675, 1556
Bsp119I TTCGAA 3 cut(s) 88, 2071, 3123
Bsp1286I GDGCHC 3 cut(s) 424, 1417, 1582
Bsp1407I TGTACA 1 cut(s) 1952
Bsp19I CCATGG 1 cut(s) 640
BspACI CCGC 6 cut(s) 574, 649, 856, 1148, 1535, 2369
BspANI GGCC 6 cut(s) 380, 503, 759, 1701, 2208, 3192
BspCNI CTCAG 4 cut(s) 1150, 2499, 2559, 2926
BspHI TCATGA 1 cut(s) 304
BspLI GGNNCC 6 cut(s) 315, 638, 1702, 1901, 3094, 3095
BspMAI CTGCAG 2 cut(s) 2893, 3179
BspMI ACCTGC 3 cut(s) 399, 1512, 1857
BspPI GGATC 8 cut(s) 221, 308, 321, 351, 631, 644, 1362, 1772
BspQI GCTCTTC 1 cut(s) 2352
BspT104I TTCGAA 3 cut(s) 88, 2071, 3123
BsrDI GCAATG 2 cut(s) 1311, 1980
BsrFI RCCGGY 1 cut(s) 3069
BsrGI TGTACA 1 cut(s) 1952
BsrI ACTGG 9 cut(s) 69, 296, 944, 1033, 1704, 2209, 2963, 3056, 3307
BssAI RCCGGY 1 cut(s) 3069
BssECI CCNNGG 6 cut(s) 230, 640, 950, 1715, 1781, 1922
BssSI CACGAG 1 cut(s) 1038
BssT1I CCWWGG 3 cut(s) 230, 640, 1781
Bst2BI CACGAG 1 cut(s) 1038
Bst2UI CCWGG 6 cut(s) 951, 1509, 1716, 1923, 1991, 2919
Bst4CI ACNGT 9 cut(s) 85, 289, 670, 1121, 1376, 1426, 1814, 1864, 2555
Bst6I CTCTTC 6 cut(s) 369, 541, 2240, 2352, 2712, 2877
BstAPI GCANNNNNTGC 1 cut(s) 3026
BstAUI TGTACA 1 cut(s) 1952
BstBI TTCGAA 3 cut(s) 88, 2071, 3123
BstC8I GCNNGC 3 cut(s) 647, 1152, 3154
BstDEI CTNAG 8 cut(s) 62, 1137, 1597, 2486, 2546, 2621, 2934, 3135
BstDSI CCRYGG 1 cut(s) 640
BstENI CCTNNNNNAGG 1 cut(s) 2889
BstH2I RGCGCY 1 cut(s) 1194
BstHHI GCGC 2 cut(s) 408, 1193
BstMAI GTCTC 7 cut(s) 409, 1651, 2156, 2242, 2253, 2294, 3232
BstMWI GCNNNNNNNGC 7 cut(s) 651, 853, 1518, 1586, 1815, 2671, 3026
BstNI CCWGG 6 cut(s) 951, 1509, 1716, 1923, 1991, 2919
BstNSI RCATGY 3 cut(s) 649, 1320, 1919
BstSCI CCNGG 6 cut(s) 949, 1507, 1714, 1921, 1989, 2917
BstSFI CTRYAG 5 cut(s) 1752, 1810, 2169, 2889, 3175
BstSLI GKGCMC 1 cut(s) 1582
BstV1I GCAGC 4 cut(s) 622, 704, 2579, 3189
BstV2I GAAGAC 5 cut(s) 49, 84, 2033, 2636, 3107
BstX2I RGATCY 4 cut(s) 313, 636, 2867, 3049
BstYI RGATCY 4 cut(s) 313, 636, 2867, 3049
BsuI GTATCC 1 cut(s) 1758
BsuRI GGCC 6 cut(s) 380, 503, 759, 1701, 2208, 3192
BtgI CCRYGG 1 cut(s) 640
BtrI CACGTC 1 cut(s) 419
BtsI GCAGTG 3 cut(s) 1624, 2929, 3102
BtsIMutI CAGTG 8 cut(s) 76, 1117, 1624, 2554, 2929, 2943, 3102, 3300
BveI ACCTGC 3 cut(s) 399, 1512, 1857
Cac8I GCNNGC 3 cut(s) 647, 1152, 3154
CaiI CAGNNNCTG 1 cut(s) 2553
CciI TCATGA 1 cut(s) 304
CfoI GCGC 2 cut(s) 408, 1193
Cfr10I RCCGGY 1 cut(s) 3069
Cfr13I GGNCC 7 cut(s) 121, 501, 947, 1700, 1899, 2960, 3093
CseI GACGC 1 cut(s) 2276
Csp6I GTAC 6 cut(s) 220, 290, 606, 1086, 1553, 1953
CspCI CAANNNNNGTGG 4 cut(s) 1944, 1979, 2281, 2316
CviQI GTAC 6 cut(s) 220, 290, 606, 1086, 1553, 1953
DdeI CTNAG 8 cut(s) 62, 1137, 1597, 2486, 2546, 2621, 2934, 3135
DraI TTTAAA 5 cut(s) 385, 1068, 1327, 2274, 3289
Eam1104I CTCTTC 6 cut(s) 369, 541, 2240, 2352, 2712, 2877
EarI CTCTTC 6 cut(s) 369, 541, 2240, 2352, 2712, 2877
EciI GGCGGA 1 cut(s) 871
Ecl136II GAGCTC 1 cut(s) 1415
Eco130I CCWWGG 3 cut(s) 230, 640, 1781
Eco147I AGGCCT 1 cut(s) 380
Eco24I GRGCYC 1 cut(s) 1417
Eco32I GATATC 1 cut(s) 2480
Eco47I GGWCC 5 cut(s) 121, 947, 1899, 2960, 3093
Eco47III AGCGCT 1 cut(s) 1192
Eco53kI GAGCTC 1 cut(s) 1415
Eco57I CTGAAG 5 cut(s) 1646, 2264, 2546, 2728, 2858
Eco88I CYCGRG 2 cut(s) 675, 1556
EcoICRI GAGCTC 1 cut(s) 1415
EcoNI CCTNNNNNAGG 1 cut(s) 2889
EcoO109I RGGNCCY 3 cut(s) 1700, 1899, 3093
EcoRI GAATTC 1 cut(s) 2880
EcoRII CCWGG 6 cut(s) 949, 1507, 1714, 1921, 1989, 2917
EcoRV GATATC 1 cut(s) 2480
EcoT14I CCWWGG 3 cut(s) 230, 640, 1781
EcoT22I ATGCAT 2 cut(s) 1452, 3022
EcoT38I GRGCYC 1 cut(s) 1417
ErhI CCWWGG 3 cut(s) 230, 640, 1781
Esp3I CGTCTC 2 cut(s) 409, 2294
FalI AAGNNNNNCTT 4 cut(s) 765, 797, 1867, 1899
FaqI GGGAC 3 cut(s) 950, 2263, 3106
FauI CCCGC 2 cut(s) 581, 642
FauNDI CATATG 1 cut(s) 602
FbaI TGATCA 1 cut(s) 2947
FblI GTMKAC 1 cut(s) 865
Fnu4HI GCNGC 5 cut(s) 611, 718, 2370, 2593, 3178
FriOI GRGCYC 1 cut(s) 1417
Fsp4HI GCNGC 5 cut(s) 611, 718, 2370, 2593, 3178
FspBI CTAG 5 cut(s) 518, 761, 1280, 1418, 1782
FspI TGCGCA 1 cut(s) 407
GlaI GCGC 2 cut(s) 407, 1192
GluI GCNGC 5 cut(s) 611, 718, 2370, 2593, 3178
GsuI CTGGAG 2 cut(s) 1905, 2012
HaeII RGCGCY 1 cut(s) 1194
HaeIII GGCC 6 cut(s) 380, 503, 759, 1701, 2208, 3192
HapII CCGG 3 cut(s) 395, 2579, 3070
HgaI GACGC 1 cut(s) 2276
HhaI GCGC 2 cut(s) 408, 1193
Hin6I GCGC 2 cut(s) 406, 1191
HinP1I GCGC 2 cut(s) 406, 1191
HincII GTYRAC 3 cut(s) 811, 906, 1035
HindII GTYRAC 3 cut(s) 811, 906, 1035
HindIII AAGCTT 3 cut(s) 2051, 2672, 2734
HpaI GTTAAC 2 cut(s) 811, 1035
HpaII CCGG 3 cut(s) 395, 2579, 3070
HphI GGTGA 7 cut(s) 164, 173, 533, 1055, 1979, 3245, 3325
Hpy188I TCNGA 7 cut(s) 322, 1263, 2202, 2244, 2660, 2802, 2935
Hpy99I CGWCG 1 cut(s) 3048
HpyCH4III ACNGT 9 cut(s) 85, 289, 670, 1121, 1376, 1426, 1814, 1864, 2555
HpyCH4IV ACGT 1 cut(s) 418
HpyF10VI GCNNNNNNNGC 7 cut(s) 651, 853, 1518, 1586, 1815, 2671, 3026
HpyF3I CTNAG 8 cut(s) 62, 1137, 1597, 2486, 2546, 2621, 2934, 3135
HpySE526I ACGT 1 cut(s) 418
HspAI GCGC 2 cut(s) 406, 1191
KflI GGGWCCC 1 cut(s) 3093
Ksp22I TGATCA 1 cut(s) 2947
KspAI GTTAAC 2 cut(s) 811, 1035
LguI GCTCTTC 1 cut(s) 2352
LmnI GCTCC 6 cut(s) 40, 836, 1924, 2536, 2816, 2977
Lsp1109I GCAGC 4 cut(s) 622, 704, 2579, 3189
LweI GCATC 8 cut(s) 232, 1000, 1437, 1641, 2294, 2356, 2664, 2825
MaeI CTAG 5 cut(s) 518, 761, 1280, 1418, 1782
MaeII ACGT 1 cut(s) 418
MaeIII GTNAC 4 cut(s) 1246, 1348, 1420, 2549
MfeI CAATTG 4 cut(s) 177, 198, 455, 1212
MflI RGATCY 4 cut(s) 313, 636, 2867, 3049
MhlI GDGCHC 3 cut(s) 424, 1417, 1582
MlyI GAGTC 5 cut(s) 1540, 1595, 2698, 3209, 3336
MmeI TCCRAC 1 cut(s) 1395
Mph1103I ATGCAT 2 cut(s) 1452, 3022
MroXI GAANNNNTTC 6 cut(s) 568, 780, 1050, 2040, 2884, 3264
MslI CAYNNNNRTG 2 cut(s) 563, 3305
MspA1I CMGCKG 1 cut(s) 1150
MspI CCGG 3 cut(s) 395, 2579, 3070
MspR9I CCNGG 6 cut(s) 951, 1509, 1716, 1923, 1991, 2919
MunI CAATTG 4 cut(s) 177, 198, 455, 1212
Mva1269I GAATGC 2 cut(s) 269, 1640
MvaI CCWGG 6 cut(s) 951, 1509, 1716, 1923, 1991, 2919
MwoI GCNNNNNNNGC 7 cut(s) 651, 853, 1518, 1586, 1815, 2671, 3026
NcoI CCATGG 1 cut(s) 640
NdeI CATATG 1 cut(s) 602
NlaIV GGNNCC 6 cut(s) 315, 638, 1702, 1901, 3094, 3095
NmuCI GTSAC 1 cut(s) 2549
NsbI TGCGCA 1 cut(s) 407
NsiI ATGCAT 2 cut(s) 1452, 3022
NspI RCATGY 3 cut(s) 649, 1320, 1919
NspV TTCGAA 3 cut(s) 88, 2071, 3123
PaeI GCATGC 1 cut(s) 649
PaeR7I CTCGAG 2 cut(s) 675, 1556
PagI TCATGA 1 cut(s) 304
PaqCI CACCTGC 1 cut(s) 399
PceI AGGCCT 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 2352
PctI GAATGC 2 cut(s) 269, 1640
PdmI GAANNNNTTC 6 cut(s) 568, 780, 1050, 2040, 2884, 3264
PfeI GAWTC 6 cut(s) 185, 569, 960, 2011, 3120, 3139
PkrI GCNGC 5 cut(s) 612, 719, 2371, 2594, 3179
PleI GAGTC 5 cut(s) 1539, 1595, 2698, 3208, 3335
PpsI GAGTC 5 cut(s) 1539, 1595, 2698, 3208, 3335
PpuMI RGGWCCY 2 cut(s) 1899, 3093
PshBI ATTAAT 3 cut(s) 1724, 1937, 2805
PsiI TTATAA 1 cut(s) 2969
Psp124BI GAGCTC 1 cut(s) 1417
Psp5II RGGWCCY 2 cut(s) 1899, 3093
Psp6I CCWGG 6 cut(s) 949, 1507, 1714, 1921, 1989, 2917
PspGI CCWGG 6 cut(s) 949, 1507, 1714, 1921, 1989, 2917
PspN4I GGNNCC 6 cut(s) 315, 638, 1702, 1901, 3094, 3095
PspPI GGNCC 7 cut(s) 121, 501, 947, 1700, 1899, 2960, 3093
PspPPI RGGWCCY 2 cut(s) 1899, 3093
PspXI VCTCGAGB 1 cut(s) 675
PstI CTGCAG 2 cut(s) 2893, 3179
PstNI CAGNNNCTG 1 cut(s) 2553
PsuI RGATCY 4 cut(s) 313, 636, 2867, 3049
RsaI GTAC 6 cut(s) 221, 291, 607, 1087, 1554, 1954
RsaNI GTAC 6 cut(s) 220, 290, 606, 1086, 1553, 1953
RseI CAYNNNNRTG 2 cut(s) 563, 3305
SacI GAGCTC 1 cut(s) 1417
SapI GCTCTTC 1 cut(s) 2352
SatI GCNGC 5 cut(s) 611, 718, 2370, 2593, 3178
Sau96I GGNCC 7 cut(s) 121, 501, 947, 1700, 1899, 2960, 3093
ScaI AGTACT 1 cut(s) 291
SchI GAGTC 5 cut(s) 1540, 1595, 2698, 3209, 3336
ScrFI CCNGG 6 cut(s) 951, 1509, 1716, 1923, 1991, 2919
SduI GDGCHC 3 cut(s) 424, 1417, 1582
SfaNI GCATC 8 cut(s) 232, 1000, 1437, 1641, 2294, 2356, 2664, 2825
SfcI CTRYAG 5 cut(s) 1752, 1810, 2169, 2889, 3175
Sfr274I CTCGAG 2 cut(s) 675, 1556
SfuI TTCGAA 3 cut(s) 88, 2071, 3123
SinI GGWCC 5 cut(s) 121, 947, 1899, 2960, 3093
SlaI CTCGAG 2 cut(s) 675, 1556
SmiMI CAYNNNNRTG 2 cut(s) 563, 3305
SmlI CTYRAG 4 cut(s) 675, 1500, 1556, 2282
SmoI CTYRAG 4 cut(s) 675, 1500, 1556, 2282
SphI GCATGC 1 cut(s) 649
SseBI AGGCCT 1 cut(s) 380
SsiI CCGC 6 cut(s) 574, 649, 856, 1148, 1535, 2369
SspMI CTAG 5 cut(s) 518, 761, 1280, 1418, 1782
SstI GAGCTC 1 cut(s) 1417
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 6 cut(s) 949, 1507, 1714, 1921, 1989, 2917
StyI CCWWGG 3 cut(s) 230, 640, 1781
TaaI ACNGT 9 cut(s) 85, 289, 670, 1121, 1376, 1426, 1814, 1864, 2555
TaiI ACGT 1 cut(s) 421
TaqII GACCGA 2 cut(s) 726, 935
TatI WGTACW 4 cut(s) 219, 289, 1552, 1952
TauI GCSGC 1 cut(s) 2372
TfiI GAWTC 6 cut(s) 185, 569, 960, 2011, 3120, 3139
TscAI CASTG 8 cut(s) 76, 1124, 1631, 2554, 2929, 2950, 3109, 3307
TseFI GTSAC 1 cut(s) 2549
TseI GCWGC 4 cut(s) 610, 717, 2592, 3177
Tsp45I GTSAC 1 cut(s) 2549
TspGWI ACGGA 6 cut(s) 489, 1265, 1667, 2549, 3032, 3320
TspRI CASTG 8 cut(s) 76, 1124, 1631, 2554, 2929, 2950, 3109, 3307
VneI GTGCAC 1 cut(s) 1578
VpaK11BI GGWCC 5 cut(s) 121, 947, 1899, 2960, 3093
VspI ATTAAT 3 cut(s) 1724, 1937, 2805
XagI CCTNNNNNAGG 1 cut(s) 2889
XapI RAATTY 7 cut(s) 91, 1771, 2073, 2109, 2221, 2686, 2880
XceI RCATGY 3 cut(s) 649, 1320, 1919
XcmI CCANNNNNNNNNTGG 2 cut(s) 796, 1987
XhoI CTCGAG 2 cut(s) 675, 1556
XmaJI CCTAGG 1 cut(s) 1781
XmiI GTMKAC 1 cut(s) 865
XmnI GAANNNNTTC 6 cut(s) 568, 780, 1050, 2040, 2884, 3264
XspI CTAG 5 cut(s) 518, 761, 1280, 1418, 1782
ZrmI AGTACT 1 cut(s) 291
Zsp2I ATGCAT 2 cut(s) 1452, 3022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.