RchiOBHm_Chr1g0322831

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
10246377 .. 10251390
5014 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55280

Sequence Viewer

Length: 2427 bp
ATGTCTAAAACCAATGGCCTTGTTTCTCTACAAGAAAAACTTCTTTCCAAAATCCTGATGGCAAAAGTTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATACAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAACTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAGTCATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACGTGACGTTGATGCTACATACAAGGCTAACCCGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAGGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGGATCTGTGCTACCATATTCTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCGCTGATAAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGACAAACGCATTATTGGGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTGCATATTGCTTGCTTTTACAAGGGGATGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGACCCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTTGATGCATGATTTACTACAAGCAATGGGCTGGGAAATTGTTCATGAACAGTCTCCGAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAACGTGCTGAATAGAAATACGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTACATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACCTGTCTTTCTAATTCCTTGAGACTTCTTGAATGGACTGGGTGTCCCTTAAGATCTCTCCCACAAAACTTTGAAGCCGATGAACTTGTTGAACTTAACTTGTGCCACAGCAACATTGAACAACTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAGCTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCGTTTGGTAAGAATCCATCAATCCCTTGCATTTCTCAAAAAGCTTATTGTGCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAGTCTCTTGAATCATTAATTCTTTCTAACTGTTCAAAAGTTAAGAAGATTCCTGAGTTTGTTGGAAATATGGAATGTTTGTTGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGATTCAGTTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTACGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAATTGAAGTCTATTGAAGATCTTAATCTTTCTGGATGCTTGAACCTCGGCAAACCTCAGGTAAATGTGGGGGTGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGTGGGTCTGCAATAGAAATGTCGTCTACCCATGATCGCAAGAAAAAAGGGAGAGGTCCAATCTTTCAAGGATGCAAAGTAGTTTGGCGCTCTTTCAAAAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAAAGAGCTTCCACTTGTCTATATCACAAAACGTGAATGCAGAGCCAAAGAGTTTCCACTTGCCTATATCACAAAACGTGAATGCAGAGCCAAAGAGTTTCCACTTGCCTATATCTGGTCTGTGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGATTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTTAACAGACTAGACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAACCAAGGATGCAATAATATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAAGAGTGAAACTTGTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCCGCCATCAGAGTTATGGGTGTTCTGTAAGTGTATATCTTCCTGCATATGGGAATAACGGTCGGCTAATGGGATTTTCTTTGTGTGCTGTTTTTGTACTCCATAAGTACCATCTGGTGGATAAGCTTCCTAGAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCTTGAAGCTTGATGGAAGAAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

808

Amino Acids

91.0

Weight (kDa)

7.0

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 114 2.7e-08 NB-ARC domain
WHD_ROQ1 PF23282 181 - 252 1.9e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 350 - 368 3.3e-07 Leucine Rich Repeat
LRR_13 PF23286 441 - 525 1.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 452 - 520 8.8e-10 Leucine-rich repeat region
C-JID PF20160 732 - 781 7e-11 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1163
Acc36I ACCTGC 1 cut(s) 1163
AccB7I CCANNNNNTGG 2 cut(s) 850, 2338
AccI GTMKAC 2 cut(s) 1661, 1987
AciI CCGC 2 cut(s) 115, 2224
AclWI GGATC 3 cut(s) 165, 375, 609
AcsI RAATTY 4 cut(s) 907, 1105, 2044, 2360
AcuI CTGAAG 1 cut(s) 2159
AcvI CACGTG 1 cut(s) 260
AfaI GTAC 3 cut(s) 2014, 2319, 2330
AfeI AGCGCT 1 cut(s) 451
AfiI CCNNNNNNNGG 5 cut(s) 647, 850, 1892, 2270, 2338
AflII CTTAAG 1 cut(s) 1012
AhdI GACNNNNNGTC 1 cut(s) 1005
AjnI CCWGG 3 cut(s) 208, 766, 2197
AjuI GAANNNNNNNTTGG 2 cut(s) 1919, 1951
AluBI AGCT 8 cut(s) 98, 634, 1132, 1258, 1785, 1921, 2347, 2407
AluI AGCT 8 cut(s) 98, 634, 1132, 1258, 1785, 1921, 2347, 2407
AlwI GGATC 3 cut(s) 165, 375, 609
AlwNI CAGNNNCTG 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 1292
Aor51HI AGCGCT 1 cut(s) 451
AoxI GGCC 3 cut(s) 16, 958, 1465
ApeKI GCWGC 1 cut(s) 98
ApoI RAATTY 4 cut(s) 907, 1105, 2044, 2360
AseI ATTAAT 1 cut(s) 1337
Asp700I GAANNNNTTC 5 cut(s) 39, 309, 741, 835, 2144
AspLEI GCGC 2 cut(s) 452, 1725
AspS9I GGNCC 3 cut(s) 206, 946, 1691
AsuHPI GGTGA 2 cut(s) 955, 1612
AvaI CYCGRG 1 cut(s) 1292
AvaII GGWCC 3 cut(s) 206, 946, 1691
AxyI CCTNAGG 1 cut(s) 1584
BauI CACGAG 1 cut(s) 416
BbrPI CACGTG 1 cut(s) 260
BbsI GAAGAC 4 cut(s) 804, 917, 1502, 2147
BbvI GCAGC 1 cut(s) 85
BceAI ACGGC 1 cut(s) 1472
BcgI CGANNNNNNTGC 2 cut(s) 1273, 1307
BciT130I CCWGG 3 cut(s) 210, 768, 2199
BfaI CTAG 7 cut(s) 173, 386, 620, 635, 1991, 2057, 2352
BfmI CTRYAG 1 cut(s) 1492
BfoI RGCGCY 2 cut(s) 453, 1726
BfrI CTTAAG 1 cut(s) 1012
BfuAI ACCTGC 1 cut(s) 1163
BglII AGATCT 4 cut(s) 671, 1016, 1198, 1546
BisI GCNGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 100
Bme1390I CCNGG 3 cut(s) 210, 768, 2199
Bme18I GGWCC 3 cut(s) 206, 946, 1691
BmeRI GACNNNNNGTC 1 cut(s) 1005
BmeT110I CYCGRG 1 cut(s) 1292
BmgT120I GGNCC 3 cut(s) 206, 946, 1691
BmiI GGNNCC 1 cut(s) 2222
BmrFI CCNGG 3 cut(s) 210, 768, 2199
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 7 cut(s) 259, 696, 1553, 1697, 2026, 2048, 2102
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 4 cut(s) 804, 917, 1502, 2147
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 3 cut(s) 1003, 1208, 1943
BsaAI YACGTR 1 cut(s) 260
BsaBI GATNNNNATC 3 cut(s) 156, 600, 1551
BsaI GGTCTC 2 cut(s) 795, 1151
BsaJI CCNNGG 4 cut(s) 209, 844, 1573, 2107
Bsc4I CCNNNNNNNGG 5 cut(s) 647, 850, 1892, 2270, 2338
Bse1I ACTGG 3 cut(s) 203, 543, 1006
Bse21I CCTNAGG 1 cut(s) 1584
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 3 cut(s) 156, 600, 1551
BseBI CCWGG 3 cut(s) 210, 768, 2199
BseDI CCNNGG 4 cut(s) 209, 844, 1573, 2107
BseGI GGATG 7 cut(s) 88, 547, 597, 1214, 1568, 1712, 2117
BseJI GATNNNNATC 3 cut(s) 156, 600, 1551
BseLI CCNNNNNNNGG 5 cut(s) 647, 850, 1892, 2270, 2338
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 5 cut(s) 410, 1365, 1451, 1598, 2205
BseNI ACTGG 3 cut(s) 203, 543, 1006
BseXI GCAGC 1 cut(s) 85
BseYI CCCAGC 1 cut(s) 732
Bsh1285I CGRYCG 1 cut(s) 2284
BshFI GGCC 3 cut(s) 18, 960, 1467
BsiEI CGRYCG 1 cut(s) 2284
BsiHKCI CYCGRG 1 cut(s) 1292
BslFI GGGAC 2 cut(s) 330, 993
BslI CCNNNNNNNGG 5 cut(s) 647, 850, 1892, 2270, 2338
BsmFI GGGAC 2 cut(s) 330, 993
BsmI GAATGC 2 cut(s) 1819, 1864
BsnI GGCC 3 cut(s) 18, 960, 1467
Bso31I GGTCTC 2 cut(s) 795, 1151
BsoBI CYCGRG 1 cut(s) 1292
Bsp143I GATC 8 cut(s) 157, 367, 601, 671, 1016, 1198, 1546, 1669
BspACI CCGC 2 cut(s) 115, 2224
BspANI GGCC 3 cut(s) 18, 960, 1467
BspCNI CTCAG 5 cut(s) 409, 1366, 1452, 1597, 2206
BspHI TCATGA 2 cut(s) 745, 793
BspLI GGNNCC 1 cut(s) 2222
BspMAI CTGCAG 1 cut(s) 1496
BspMI ACCTGC 1 cut(s) 1163
BspPI GGATC 3 cut(s) 165, 375, 609
BspTI CTTAAG 1 cut(s) 1012
BspTNI GGTCTC 2 cut(s) 795, 1151
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 3 cut(s) 203, 543, 1006
BssECI CCNNGG 4 cut(s) 209, 844, 1573, 2107
BssMI GATC 8 cut(s) 157, 367, 601, 671, 1016, 1198, 1546, 1669
BssNAI GTATAC 1 cut(s) 1988
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 2 cut(s) 844, 2107
Bst1107I GTATAC 1 cut(s) 1988
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 3 cut(s) 210, 768, 2199
Bst4CI ACNGT 6 cut(s) 299, 702, 753, 1352, 1610, 2282
BstAFI CTTAAG 1 cut(s) 1012
BstBAI YACGTR 1 cut(s) 260
BstC8I GCNNGC 2 cut(s) 577, 632
BstDEI CTNAG 7 cut(s) 396, 920, 1374, 1460, 1584, 1917, 2214
BstF5I GGATG 7 cut(s) 88, 547, 597, 1214, 1568, 1712, 2117
BstH2I RGCGCY 2 cut(s) 453, 1726
BstHHI GCGC 2 cut(s) 452, 1725
BstKTI GATC 8 cut(s) 160, 370, 604, 674, 1019, 1201, 1549, 1672
BstMBI GATC 8 cut(s) 157, 367, 601, 671, 1016, 1198, 1546, 1669
BstMCI CGRYCG 1 cut(s) 2284
BstMWI GCNNNNNNNGC 2 cut(s) 1264, 1918
BstNI CCWGG 3 cut(s) 210, 768, 2199
BstSCI CCNGG 3 cut(s) 208, 766, 2197
BstSFI CTRYAG 1 cut(s) 1492
BstV1I GCAGC 1 cut(s) 85
BstV2I GAAGAC 4 cut(s) 804, 917, 1502, 2147
BstX2I RGATCY 5 cut(s) 367, 671, 1016, 1198, 1546
BstYI RGATCY 5 cut(s) 367, 671, 1016, 1198, 1546
BstZ17I GTATAC 1 cut(s) 1988
Bsu36I CCTNAGG 1 cut(s) 1584
BsuRI GGCC 3 cut(s) 18, 960, 1467
BtsCI GGATG 7 cut(s) 88, 547, 597, 1214, 1568, 1712, 2117
BtsIMutI CAGTG 1 cut(s) 2018
BveI ACCTGC 1 cut(s) 1163
Cac8I GCNNGC 2 cut(s) 577, 632
CaiI CAGNNNCTG 1 cut(s) 98
CciI TCATGA 2 cut(s) 745, 793
CfoI GCGC 2 cut(s) 452, 1725
Cfr13I GGNCC 3 cut(s) 206, 946, 1691
Csp6I GTAC 3 cut(s) 2013, 2318, 2329
CviAII CATG 6 cut(s) 710, 746, 794, 850, 1667, 2149
CviQI GTAC 3 cut(s) 2013, 2318, 2329
DdeI CTNAG 7 cut(s) 396, 920, 1374, 1460, 1584, 1917, 2214
DpnI GATC 8 cut(s) 159, 369, 603, 673, 1018, 1200, 1548, 1671
DpnII GATC 8 cut(s) 157, 367, 601, 671, 1016, 1198, 1546, 1669
DriI GACNNNNNGTC 1 cut(s) 1005
Eam1105I GACNNNNNGTC 1 cut(s) 1005
EciI GGCGGA 1 cut(s) 2213
Eco130I CCWWGG 2 cut(s) 844, 2107
Eco147I AGGCCT 1 cut(s) 960
Eco31I GGTCTC 2 cut(s) 795, 1151
Eco47I GGWCC 3 cut(s) 206, 946, 1691
Eco47III AGCGCT 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 2159
Eco72I CACGTG 1 cut(s) 260
Eco81I CCTNAGG 1 cut(s) 1584
Eco88I CYCGRG 1 cut(s) 1292
EcoRI GAATTC 1 cut(s) 2360
EcoRII CCWGG 3 cut(s) 208, 766, 2197
EcoT14I CCWWGG 2 cut(s) 844, 2107
EcoT22I ATGCAT 2 cut(s) 711, 2041
ErhI CCWWGG 2 cut(s) 844, 2107
FaeI CATG 6 cut(s) 713, 749, 797, 853, 1670, 2152
FalI AAGNNNNNCTT 2 cut(s) 24, 56
FaqI GGGAC 2 cut(s) 330, 993
FatI CATG 6 cut(s) 709, 745, 793, 849, 1666, 2148
FauNDI CATATG 1 cut(s) 2269
FblI GTMKAC 2 cut(s) 1661, 1987
Fnu4HI GCNGC 1 cut(s) 99
FokI GGATG 7 cut(s) 95, 554, 604, 1221, 1575, 1719, 2124
Fsp4HI GCNGC 1 cut(s) 99
FspBI CTAG 7 cut(s) 173, 386, 620, 635, 1991, 2057, 2352
GlaI GCGC 2 cut(s) 451, 1724
GluI GCNGC 1 cut(s) 99
GsaI CCCAGC 1 cut(s) 736
HaeII RGCGCY 2 cut(s) 453, 1726
HaeIII GGCC 3 cut(s) 18, 960, 1467
HhaI GCGC 2 cut(s) 452, 1725
Hin1II CATG 6 cut(s) 713, 749, 797, 853, 1670, 2152
Hin6I GCGC 2 cut(s) 450, 1723
HinP1I GCGC 2 cut(s) 450, 1723
HincII GTYRAC 2 cut(s) 165, 864
HindII GTYRAC 2 cut(s) 165, 864
HindIII AAGCTT 4 cut(s) 1256, 1919, 2345, 2405
HphI GGTGA 2 cut(s) 955, 1612
Hpy166II GTNNAC 5 cut(s) 165, 864, 946, 1662, 1988
Hpy188I TCNGA 5 cut(s) 522, 759, 1182, 1969, 2232
Hpy8I GTNNAC 5 cut(s) 165, 864, 946, 1662, 1988
HpyAV CCTTC 5 cut(s) 959, 1087, 1199, 1755, 1982
HpyCH4III ACNGT 6 cut(s) 299, 702, 753, 1352, 1610, 2282
HpyCH4IV ACGT 6 cut(s) 259, 264, 775, 810, 1809, 1854
HpyF10VI GCNNNNNNNGC 2 cut(s) 1264, 1918
HpyF3I CTNAG 7 cut(s) 396, 920, 1374, 1460, 1584, 1917, 2214
HpySE526I ACGT 6 cut(s) 259, 264, 775, 810, 1809, 1854
Hsp92II CATG 6 cut(s) 713, 749, 797, 853, 1670, 2152
HspAI GCGC 2 cut(s) 450, 1723
Kzo9I GATC 8 cut(s) 157, 367, 601, 671, 1016, 1198, 1546, 1669
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 7 cut(s) 259, 696, 1553, 1697, 2026, 2048, 2102
MaeI CTAG 7 cut(s) 173, 386, 620, 635, 1991, 2057, 2352
MaeII ACGT 6 cut(s) 259, 264, 775, 810, 1809, 1854
MaeIII GTNAC 2 cut(s) 260, 607
MalI GATC 8 cut(s) 159, 369, 603, 673, 1018, 1200, 1548, 1671
MboI GATC 8 cut(s) 157, 367, 601, 671, 1016, 1198, 1546, 1669
MboII GAAGA 9 cut(s) 368, 809, 917, 1378, 1502, 1556, 2152, 2252, 2427
MfeI CAATTG 1 cut(s) 2083
MflI RGATCY 5 cut(s) 367, 671, 1016, 1198, 1546
MlyI GAGTC 3 cut(s) 228, 1306, 1331
MmeI TCCRAC 2 cut(s) 714, 1363
MnlI CCTC 9 cut(s) 73, 321, 971, 1178, 1426, 1583, 1593, 1609, 1682
Mph1103I ATGCAT 2 cut(s) 711, 2041
MroXI GAANNNNTTC 5 cut(s) 39, 309, 741, 835, 2144
MslI CAYNNNNRTG 1 cut(s) 854
MspCI CTTAAG 1 cut(s) 1012
MspR9I CCNGG 3 cut(s) 210, 768, 2199
MunI CAATTG 1 cut(s) 2083
Mva1269I GAATGC 2 cut(s) 1819, 1864
MvaI CCWGG 3 cut(s) 210, 768, 2199
MwoI GCNNNNNNNGC 2 cut(s) 1264, 1918
NdeI CATATG 1 cut(s) 2269
NdeII GATC 8 cut(s) 157, 367, 601, 671, 1016, 1198, 1546, 1669
NlaIII CATG 6 cut(s) 713, 749, 797, 853, 1670, 2152
NlaIV GGNNCC 1 cut(s) 2222
NmeAIII GCCGAG 1 cut(s) 1554
NmuCI GTSAC 2 cut(s) 260, 607
NsiI ATGCAT 2 cut(s) 711, 2041
PaeR7I CTCGAG 1 cut(s) 1292
PagI TCATGA 2 cut(s) 745, 793
PaqCI CACCTGC 1 cut(s) 1163
PceI AGGCCT 1 cut(s) 960
PctI GAATGC 2 cut(s) 1819, 1864
PdmI GAANNNNTTC 5 cut(s) 39, 309, 741, 835, 2144
PfeI GAWTC 9 cut(s) 836, 1183, 1227, 1270, 1331, 1369, 1445, 1480, 2160
PflFI GACNNNGTC 1 cut(s) 641
PflMI CCANNNNNTGG 2 cut(s) 850, 2338
PfoI TCCNGGA 1 cut(s) 2197
PkrI GCNGC 1 cut(s) 100
PleI GAGTC 3 cut(s) 227, 1305, 1330
PmaCI CACGTG 1 cut(s) 260
PmlI CACGTG 1 cut(s) 260
PpsI GAGTC 3 cut(s) 227, 1305, 1330
Ppu21I YACGTR 1 cut(s) 260
PshBI ATTAAT 1 cut(s) 1337
Psp6I CCWGG 3 cut(s) 208, 766, 2197
PspCI CACGTG 1 cut(s) 260
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 3 cut(s) 208, 766, 2197
PspN4I GGNNCC 1 cut(s) 2222
PspPI GGNCC 3 cut(s) 206, 946, 1691
PstI CTGCAG 1 cut(s) 1496
PstNI CAGNNNCTG 1 cut(s) 98
PsuI RGATCY 5 cut(s) 367, 671, 1016, 1198, 1546
PsyI GACNNNGTC 1 cut(s) 641
RsaI GTAC 3 cut(s) 2014, 2319, 2330
RsaNI GTAC 3 cut(s) 2013, 2318, 2329
RseI CAYNNNNRTG 1 cut(s) 854
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 8 cut(s) 157, 367, 601, 671, 1016, 1198, 1546, 1669
Sau96I GGNCC 3 cut(s) 206, 946, 1691
SchI GAGTC 3 cut(s) 228, 1306, 1331
ScrFI CCNGG 3 cut(s) 210, 768, 2199
SfaNI GCATC 7 cut(s) 259, 696, 1553, 1697, 2026, 2048, 2102
SfcI CTRYAG 1 cut(s) 1492
Sfr274I CTCGAG 1 cut(s) 1292
SinI GGWCC 3 cut(s) 206, 946, 1691
SlaI CTCGAG 1 cut(s) 1292
SmiMI CAYNNNNRTG 1 cut(s) 854
SmlI CTYRAG 5 cut(s) 982, 1012, 1187, 1292, 1922
SmoI CTYRAG 5 cut(s) 982, 1012, 1187, 1292, 1922
SseBI AGGCCT 1 cut(s) 960
SsiI CCGC 2 cut(s) 115, 2224
SspI AATATT 2 cut(s) 561, 2209
SspMI CTAG 7 cut(s) 173, 386, 620, 635, 1991, 2057, 2352
StuI AGGCCT 1 cut(s) 960
StyD4I CCNGG 3 cut(s) 208, 766, 2197
StyI CCWWGG 2 cut(s) 844, 2107
TaaI ACNGT 6 cut(s) 299, 702, 753, 1352, 1610, 2282
TaiI ACGT 6 cut(s) 262, 267, 778, 813, 1812, 1857
TaqI TCGA 1 cut(s) 1293
TatI WGTACW 2 cut(s) 2012, 2317
TfiI GAWTC 9 cut(s) 836, 1183, 1227, 1270, 1331, 1369, 1445, 1480, 2160
TscAI CASTG 1 cut(s) 2025
TseFI GTSAC 2 cut(s) 260, 607
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 2 cut(s) 260, 607
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 1 cut(s) 2025
Tth111I GACNNNGTC 1 cut(s) 641
Van91I CCANNNNNTGG 2 cut(s) 850, 2338
Vha464I CTTAAG 1 cut(s) 1012
VpaK11BI GGWCC 3 cut(s) 206, 946, 1691
VspI ATTAAT 1 cut(s) 1337
XapI RAATTY 4 cut(s) 907, 1105, 2044, 2360
XcmI CCANNNNNNNNNTGG 2 cut(s) 55, 2234
XhoI CTCGAG 1 cut(s) 1292
XmiI GTMKAC 2 cut(s) 1661, 1987
XmnI GAANNNNTTC 5 cut(s) 39, 309, 741, 835, 2144
XspI CTAG 7 cut(s) 173, 386, 620, 635, 1991, 2057, 2352
Zsp2I ATGCAT 2 cut(s) 711, 2041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.