RLG00000024652

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000105
Physical Location & Seq
Forward (+)
37908019 .. 37911092
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024652

Sequence Viewer

Length: 2229 bp
ATGGCGTCAATCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGGGGGATAGATACGCGCGGGTCTTTAACCGATCATCTGTATTCTGCACTAAATCAGAAAGGAATTCTTACCTTTCGGGATGATGCAGAGCTTGAGAAAGGAAAATCCATTAGGCCGGAACTTTTAGCTGCAATCGAGGAGTCTAGATCTGCGATTGTCATTCTCTCACCCAGATATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATGGGTCAACAAGTCCTCCCCGTCTTCTACTGGGTGGATCCTTCTGAAGTGCGGCACCAAACGGGAAGTTTTGAGCTCAGTGTGAAACCGAAACCGAAAGTGGAAGTGAGGGAACATGAAGAAGTTTATGGGAAGAATGACGACAGACTAAATGCGTGGACAGCTGCTCTGACAGAGGTGGCCAAGCTTTCTGGCTGGGTTTCCAAGAGTTATTCACATGAATCAACTTTAATTGAGGAAATTGTTGAACATATACTAAAGAAGTCCGTGTATACCTCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTATTAAACAATTACATTTATCCGCAGCTTTGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATAAAATTTGTCAGGATTTTGACCAAAGCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCATAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATTGCAAAAGTTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAAAAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCACAACTGGTTTGGTCCAGGGAGTAGAATTATCATAACCACAACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTAAACTTTTGAGTTTGAAGGCTTTCAAGAAATGTCCCCCACCAGAAAATTATTTGCATCTGTGCTACGAAATTATAGGATATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTCTTAGGTTCTTTTCTATTTGGTAGAAGCACTGATGAATGGGAAAGTGCAATAGATAGGCTAAAGAACACACCACATAAACGAATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGAAGAAAAAGACAAAGAAATCTTCCTACATATCGCTTGCTTTTACAAGGGGAGGGATAAGGATCGTGTGACACAAATACTGGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTAGCTGATAGATCTCTCATAACTATCTCCAACAACGAATTGTCGATGCATGATTTGCTACAAGAAACGGGTTGGGAAATTGTTCGTGAGGAGTTTCCCAAAGAGCCAGGCAAACGTAGTAGGTTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAGAAATAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCCAGAGTCTGCCTAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCTGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTTCCTATTTCAATTGAACGTCTGACTGGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTATGTCTTCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAAGCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAACTGATGTGAGTAGTGGGTCTGCAATAGAAACGTCATCTACCCATGATCTCATTAAATATGTGAGCGGTTCAATCTTTCATGGTTGCAAAGTAGTTTGGCGATCTTTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

743

Amino Acids

83.96

Weight (kDa)

6.5

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 191 1.5e-51 TIR domain
TIR_2 PF13676 14 - 107 3.9e-13 TIR domain
NB-ARC PF00931 216 - 371 9.4e-21 NB-ARC domain
NACHT PF05729 221 - 318 8.7e-08 NACHT domain
WHD_ROQ1 PF23282 438 - 507 1.1e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 606 - 691 9e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 617 - 687 1.3e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1658
Acc36I ACCTGC 1 cut(s) 1658
AccB1I GGYRCC 1 cut(s) 342
AccBSI CCGCTC 1 cut(s) 2181
AccI GTMKAC 1 cut(s) 560
AccII CGCG 2 cut(s) 70, 72
AciI CCGC 7 cut(s) 72, 340, 604, 638, 679, 886, 2181
AclI AACGTT 1 cut(s) 1891
AclWI GGATC 6 cut(s) 320, 333, 661, 674, 936, 1380
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 3 cut(s) 117, 723, 1600
AcuI CTGAAG 2 cut(s) 354, 1607
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 1418
AjnI CCWGG 2 cut(s) 979, 1537
AjuI GAANNNNNNNTTGG 2 cut(s) 735, 767
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 5 cut(s) 1566, 1646, 1679, 1790, 1910
AlwI GGATC 6 cut(s) 320, 333, 661, 674, 936, 1380
AlwNI CAGNNNCTG 2 cut(s) 869, 1795
Ama87I CYCGRG 1 cut(s) 705
AoxI GGCC 3 cut(s) 167, 468, 1960
ApeKI GCWGC 5 cut(s) 182, 452, 640, 747, 869
ApoI RAATTY 3 cut(s) 117, 723, 1600
AseI ATTAAT 1 cut(s) 1832
Asp700I GAANNNNTTC 4 cut(s) 598, 810, 1103, 1512
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 1 cut(s) 977
AsuHPI GGTGA 1 cut(s) 213
AvaI CYCGRG 1 cut(s) 705
AvaII GGWCC 1 cut(s) 977
BalI TGGCCA 1 cut(s) 470
BamHI GGATCC 2 cut(s) 325, 666
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1187
BbsI GAAGAC 3 cut(s) 304, 1575, 1997
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 5 cut(s) 169, 439, 652, 734, 856
BccI CCATC 5 cut(s) 283, 1298, 1734, 2024, 2092
BciT130I CCWGG 2 cut(s) 981, 1539
BcoDI GTCTC 5 cut(s) 1566, 1646, 1679, 1790, 1910
BfaI CTAG 3 cut(s) 198, 1433, 1800
BfmI CTRYAG 1 cut(s) 1987
BfuAI ACCTGC 1 cut(s) 1658
BglII AGATCT 3 cut(s) 200, 1442, 1693
BisI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
BlsI GCNGC 6 cut(s) 184, 342, 454, 642, 749, 871
Bme1390I CCNGG 2 cut(s) 981, 1539
Bme18I GGWCC 1 cut(s) 977
BmeT110I CYCGRG 1 cut(s) 705
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 3 cut(s) 327, 344, 668
BmrFI CCNGG 2 cut(s) 981, 1539
BmrI ACTGGG 1 cut(s) 328
BmsI GCATC 5 cut(s) 127, 1030, 1147, 1467, 2048
BmuI ACTGGG 1 cut(s) 328
BpiI GAAGAC 3 cut(s) 304, 1575, 1997
BpmI CTGGAG 1 cut(s) 1772
Bpu10I CCTNAGC 1 cut(s) 1167
BpuEI CTTGAG 2 cut(s) 167, 1703
BsaBI GATNNNNATC 2 cut(s) 927, 1371
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1566, 1646
BsaJI CCNNGG 3 cut(s) 670, 980, 1735
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 1418
Bse1I ACTGG 5 cut(s) 323, 974, 1314, 1395, 1963
Bse8I GATNNNNATC 2 cut(s) 927, 1371
BseBI CCWGG 2 cut(s) 981, 1539
BseDI CCNNGG 3 cut(s) 670, 980, 1735
BseGI GGATG 5 cut(s) 139, 259, 859, 1709, 2063
BseJI GATNNNNATC 2 cut(s) 927, 1371
BseLI CCNNNNNNNGG 1 cut(s) 1418
BseMII CTCAG 3 cut(s) 379, 1181, 1860
BseNI ACTGG 5 cut(s) 323, 974, 1314, 1395, 1963
BseRI GAGGAG 2 cut(s) 206, 1535
BseXI GCAGC 5 cut(s) 169, 439, 652, 734, 856
BseYI CCCAGC 1 cut(s) 483
BsgI GTGCAG 1 cut(s) 84
Bsh1236I CGCG 2 cut(s) 70, 72
BshFI GGCC 3 cut(s) 169, 470, 1962
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 705
BsiSI CCGG 1 cut(s) 170
BslFI GGGAC 1 cut(s) 1101
BslI CCNNNNNNNGG 1 cut(s) 1418
BsmAI GTCTC 5 cut(s) 1566, 1646, 1679, 1790, 1910
BsmFI GGGAC 1 cut(s) 1101
BsnI GGCC 3 cut(s) 169, 470, 1962
Bso31I GGTCTC 2 cut(s) 1566, 1646
BsoBI CYCGRG 1 cut(s) 705
Bsp1286I GDGCHC 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 670
BspACI CCGC 7 cut(s) 72, 340, 604, 638, 679, 886, 2181
BspANI GGCC 3 cut(s) 169, 470, 1962
BspCNI CTCAG 3 cut(s) 378, 1180, 1861
BspFNI CGCG 2 cut(s) 70, 72
BspHI TCATGA 1 cut(s) 1564
BspLI GGNNCC 3 cut(s) 327, 344, 668
BspMAI CTGCAG 1 cut(s) 1991
BspMI ACCTGC 1 cut(s) 1658
BspPI GGATC 6 cut(s) 320, 333, 661, 674, 936, 1380
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 2 cut(s) 1566, 1646
BsrBI CCGCTC 1 cut(s) 2181
BsrI ACTGG 5 cut(s) 323, 974, 1314, 1395, 1963
BssECI CCNNGG 3 cut(s) 670, 980, 1735
BssNAI GTATAC 1 cut(s) 561
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 2 cut(s) 670, 1735
Bst1107I GTATAC 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 2 cut(s) 981, 1539
Bst4CI ACNGT 2 cut(s) 1070, 2105
Bst6I CTCTTC 3 cut(s) 19, 571, 1583
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1486
BstC8I GCNNGC 3 cut(s) 677, 1348, 1403
BstDEI CTNAG 6 cut(s) 47, 365, 1167, 1195, 1869, 1979
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 5 cut(s) 139, 259, 859, 1709, 2063
BstFNI CGCG 2 cut(s) 70, 72
BstHHI GCGC 1 cut(s) 72
BstMAI GTCTC 5 cut(s) 1566, 1646, 1679, 1790, 1910
BstMWI GCNNNNNNNGC 3 cut(s) 449, 681, 1486
BstNI CCWGG 2 cut(s) 981, 1539
BstNSI RCATGY 1 cut(s) 679
BstSCI CCNGG 2 cut(s) 979, 1537
BstSFI CTRYAG 1 cut(s) 1987
BstUI CGCG 2 cut(s) 70, 72
BstV1I GCAGC 5 cut(s) 169, 439, 652, 734, 856
BstV2I GAAGAC 3 cut(s) 304, 1575, 1997
BstX2I RGATCY 5 cut(s) 200, 325, 666, 1442, 1693
BstYI RGATCY 5 cut(s) 200, 325, 666, 1442, 1693
BstZ17I GTATAC 1 cut(s) 561
BsuRI GGCC 3 cut(s) 169, 470, 1962
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 5 cut(s) 139, 259, 859, 1709, 2063
BtsIMutI CAGTG 2 cut(s) 373, 1221
BveI ACCTGC 1 cut(s) 1658
Cac8I GCNNGC 3 cut(s) 677, 1348, 1403
CaiI CAGNNNCTG 2 cut(s) 869, 1795
CciI TCATGA 1 cut(s) 1564
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 977
DdeI CTNAG 6 cut(s) 47, 365, 1167, 1195, 1869, 1979
DraI TTTAAA 1 cut(s) 2223
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 3 cut(s) 19, 571, 1583
EarI CTCTTC 3 cut(s) 19, 571, 1583
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 670, 1735
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 2 cut(s) 1566, 1646
Eco47I GGWCC 1 cut(s) 977
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 2 cut(s) 354, 1607
Eco88I CYCGRG 1 cut(s) 705
EcoICRI GAGCTC 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 2 cut(s) 979, 1537
EcoT14I CCWWGG 2 cut(s) 670, 1735
EcoT22I ATGCAT 2 cut(s) 281, 1482
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 2 cut(s) 670, 1735
FalI AAGNNNNNCTT 4 cut(s) 105, 137, 795, 827
FaqI GGGAC 1 cut(s) 1101
FauI CCCGC 3 cut(s) 65, 611, 672
FblI GTMKAC 1 cut(s) 560
Fnu4HI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
FokI GGATG 5 cut(s) 146, 266, 866, 1716, 2070
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
FspBI CTAG 3 cut(s) 198, 1433, 1800
GlaI GCGC 1 cut(s) 71
GluI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
GsaI CCCAGC 1 cut(s) 487
GsuI CTGGAG 1 cut(s) 1772
HaeIII GGCC 3 cut(s) 169, 470, 1962
HapII CCGG 1 cut(s) 170
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 3 cut(s) 296, 577, 936
HindII GTYRAC 3 cut(s) 296, 577, 936
HindIII AAGCTT 3 cut(s) 473, 1751, 2042
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 213
Hpy166II GTNNAC 5 cut(s) 296, 447, 561, 577, 936
Hpy188I TCNGA 6 cut(s) 111, 334, 459, 1293, 1677, 1956
Hpy8I GTNNAC 5 cut(s) 296, 447, 561, 577, 936
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 3 cut(s) 339, 1093, 1694
HpyCH4III ACNGT 2 cut(s) 1070, 2105
HpyCH4IV ACGT 4 cut(s) 1546, 1891, 1951, 2147
HpyF10VI GCNNNNNNNGC 3 cut(s) 449, 681, 1486
HpyF3I CTNAG 6 cut(s) 47, 365, 1167, 1195, 1869, 1979
HpySE526I ACGT 4 cut(s) 1546, 1891, 1951, 2147
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 70
LmnI GCTCC 1 cut(s) 866
Lsp1109I GCAGC 5 cut(s) 169, 439, 652, 734, 856
LweI GCATC 5 cut(s) 127, 1030, 1147, 1467, 2048
MaeI CTAG 3 cut(s) 198, 1433, 1800
MaeII ACGT 4 cut(s) 1546, 1891, 1951, 2147
MaeIII GTNAC 2 cut(s) 962, 1378
MbiI CCGCTC 1 cut(s) 2181
MfeI CAATTG 1 cut(s) 1944
MflI RGATCY 5 cut(s) 200, 325, 666, 1442, 1693
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 1 cut(s) 470
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 2 cut(s) 203, 1801
MmeI TCCRAC 2 cut(s) 1485, 1858
Mox20I TGGCCA 1 cut(s) 470
Mph1103I ATGCAT 2 cut(s) 281, 1482
MroXI GAANNNNTTC 4 cut(s) 598, 810, 1103, 1512
MscI TGGCCA 1 cut(s) 470
MslI CAYNNNNRTG 1 cut(s) 593
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 2 cut(s) 981, 1539
MunI CAATTG 1 cut(s) 1944
MvaI CCWGG 2 cut(s) 981, 1539
MvnI CGCG 2 cut(s) 70, 72
MwoI GCNNNNNNNGC 3 cut(s) 449, 681, 1486
NcoI CCATGG 1 cut(s) 670
NlaIV GGNNCC 3 cut(s) 327, 344, 668
NmeAIII GCCGAG 1 cut(s) 2049
NmuCI GTSAC 2 cut(s) 962, 1378
NsiI ATGCAT 2 cut(s) 281, 1482
NspI RCATGY 1 cut(s) 679
PaeI GCATGC 1 cut(s) 679
PaeR7I CTCGAG 1 cut(s) 705
PagI TCATGA 1 cut(s) 1564
PaqCI CACCTGC 1 cut(s) 1658
PdmI GAANNNNTTC 4 cut(s) 598, 810, 1103, 1512
PfeI GAWTC 9 cut(s) 509, 599, 822, 1678, 1722, 1765, 1817, 1864, 1975
PkrI GCNGC 6 cut(s) 184, 342, 454, 642, 749, 871
PleI GAGTC 2 cut(s) 202, 1800
PpsI GAGTC 2 cut(s) 202, 1800
PshBI ATTAAT 1 cut(s) 1832
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 1891
Psp6I CCWGG 2 cut(s) 979, 1537
PspFI CCCAGC 1 cut(s) 483
PspGI CCWGG 2 cut(s) 979, 1537
PspN4I GGNNCC 3 cut(s) 327, 344, 668
PspPI GGNCC 1 cut(s) 977
PspXI VCTCGAGB 1 cut(s) 705
PstI CTGCAG 1 cut(s) 1991
PstNI CAGNNNCTG 2 cut(s) 869, 1795
PsuI RGATCY 5 cut(s) 200, 325, 666, 1442, 1693
PvuII CAGCTG 1 cut(s) 452
RseI CAYNNNNRTG 1 cut(s) 593
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
Sau96I GGNCC 1 cut(s) 977
SchI GAGTC 2 cut(s) 203, 1801
ScrFI CCNGG 2 cut(s) 981, 1539
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 5 cut(s) 127, 1030, 1147, 1467, 2048
SfcI CTRYAG 1 cut(s) 1987
Sfr274I CTCGAG 1 cut(s) 705
SinI GGWCC 1 cut(s) 977
SlaI CTCGAG 1 cut(s) 705
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 3 cut(s) 146, 705, 1682
SmoI CTYRAG 3 cut(s) 146, 705, 1682
SphI GCATGC 1 cut(s) 679
SsiI CCGC 7 cut(s) 72, 340, 604, 638, 679, 886, 2181
SspMI CTAG 3 cut(s) 198, 1433, 1800
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 2 cut(s) 979, 1537
StyI CCWWGG 2 cut(s) 670, 1735
TaaI ACNGT 2 cut(s) 1070, 2105
TaiI ACGT 4 cut(s) 1549, 1894, 1954, 2150
TaqI TCGA 3 cut(s) 189, 706, 1475
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 9 cut(s) 509, 599, 822, 1678, 1722, 1765, 1817, 1864, 1975
TscAI CASTG 2 cut(s) 373, 1228
TseFI GTSAC 2 cut(s) 962, 1378
TseI GCWGC 5 cut(s) 182, 452, 640, 747, 869
Tsp45I GTSAC 2 cut(s) 962, 1378
TspGWI ACGGA 2 cut(s) 544, 1167
TspRI CASTG 2 cut(s) 373, 1228
VpaK11BI GGWCC 1 cut(s) 977
VspI ATTAAT 1 cut(s) 1832
XapI RAATTY 3 cut(s) 117, 723, 1600
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 679
XcmI CCANNNNNNNNNTGG 1 cut(s) 1733
XhoI CTCGAG 1 cut(s) 705
XmiI GTMKAC 1 cut(s) 560
XmnI GAANNNNTTC 4 cut(s) 598, 810, 1103, 1512
XspI CTAG 3 cut(s) 198, 1433, 1800
Zsp2I ATGCAT 2 cut(s) 281, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.