RLG00000023324

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000105
Physical Location & Seq
Reverse (-)
21929236 .. 21947003
17768 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023324

Sequence Viewer

Length: 2034 bp
ATGGCTTCAGCCTTTGCTCGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGGGGGGTAGAGACCCGCGAGTCGTTTACCCGTCTTCTGTATGCTGCACTAAATCAGAAAGGAATTGTCACCTTTATGGATGAGCCAGAGCTTGAGAAAGGAAAATCCATTGGGCCTGAACTTTTAGCTGCAATTGAGGAGTCCAGATTTGCGATTGTCATTCTCTCACCCAACTATGCTCGTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGCTGGGCCAACAAGTCCTCCCCGTCTTCTTGTGGGTGGATCCTTCTGATGTGCGGCACCAAACGGGAAGGTTTAAGCTCAAATGGGAACGCCAAGTAGAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGCAAGAATGAGGACAGACTAAATGCCTGGAGAGCTGCTCTGACAGAGGTGGCCAATCTTTCTGGCTGGGTTTACAAGAGATTTTCAAATGATTTAAAAATGATTGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATGCATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATCTATTAAGCAATTACATATGTCCACAGCTATGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGGTATGCTCAGGGGCTTCCTTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCGCTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACACATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAACGACAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAATTATCTCCAACAACGAACTGTGGATGCATGATTTGCTGCAAGAATTGGGCTGGGAAATTGTTCGGGAACAGTCTCCCAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAAAAATAAGAATTTCGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCACAGGCATTCAGAATCTTGAGAGTTTAGACCTTGAAGGATGTGAGAATTTGGTAAGAGTCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCAAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCTATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAGTTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTGTCTCTGGCTCTTGAGAGTAGTCATCCTCTTTTCCAGAGCTGCATTCTCCTCCAGAACAGTGCTCTCCTCAAGAACAGCAACAAATTGACTTGGATCTCTTTTCTTCAGATTATGATGGGAGCTTTCGAGATGGTAACCATCGATCCTACATCTGAGGCGGAGACAAAACTTATGTCCTTGGTTAAAATGGGATACAACGAATTGGAGGCTTCAATGGCAATAGAATGCGGTATTGACTCGTCACTTGTTGATTTGACTGATTTCATATCAGCTGCTCAAATTTCAGAGGCAGAAGATGGCTATCTTCCCCTTGAAGAGATGCGCTGCACCGTGCAGCATGAGCACTGTTCAGCAGCGGCAGCGCACGTGAGATGTAAAAAAGCTGGAGAGAAGCGTTTCATTGTGATCAAGCGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

678

Amino Acids

76.73

Weight (kDa)

6.09

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 191 3.9e-52 TIR domain
TIR_2 PF13676 14 - 107 1.7e-14 TIR domain
WHD_ROQ1 PF23282 281 - 352 1.5e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 449 - 534 4.1e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 460 - 528 1.2e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 342
AccII CGCG 1 cut(s) 72
AciI CCGC 8 cut(s) 70, 340, 604, 679, 1772, 1842, 1970, 2026
AclI AACGTT 1 cut(s) 1420
AclWI GGATC 7 cut(s) 320, 333, 661, 674, 909, 1715, 1751
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 3 cut(s) 1129, 1240, 1893
AcuI CTGAAG 2 cut(s) 1136, 1703
AcvI CACGTG 1 cut(s) 1981
AfeI AGCGCT 1 cut(s) 751
AfiI CCNNNNNNNGG 1 cut(s) 947
AjnI CCWGG 2 cut(s) 443, 1066
Alw21I GWGCWC 2 cut(s) 1678, 1959
Alw26I GTCTC 8 cut(s) 59, 1059, 1095, 1175, 1319, 1439, 1620, 1769
AlwI GGATC 7 cut(s) 320, 333, 661, 674, 909, 1715, 1751
Ama87I CYCGRG 1 cut(s) 1397
Aor51HI AGCGCT 1 cut(s) 751
AoxI GGCC 4 cut(s) 167, 292, 468, 1489
ApoI RAATTY 3 cut(s) 1129, 1240, 1893
ArsI GACNNNNNNTTYG 2 cut(s) 1772, 1804
AseI ATTAAT 1 cut(s) 1361
Asp700I GAANNNNTTC 3 cut(s) 598, 1041, 2009
AspLEI GCGC 3 cut(s) 752, 1938, 1978
AspS9I GGNCC 2 cut(s) 167, 292
AsuHPI GGTGA 2 cut(s) 115, 213
AvaI CYCGRG 1 cut(s) 1397
BalI TGGCCA 1 cut(s) 470
BamHI GGATCC 2 cut(s) 325, 666
BanI GGYRCC 1 cut(s) 342
BauI CACGAG 1 cut(s) 716
BbrPI CACGTG 1 cut(s) 1981
BbsI GAAGAC 4 cut(s) 80, 304, 1104, 1526
Bbv12I GWGCWC 2 cut(s) 1678, 1959
BccI CCATC 7 cut(s) 827, 1263, 1553, 1723, 1738, 1760, 1904
BceAI ACGGC 1 cut(s) 1496
BciT130I CCWGG 2 cut(s) 445, 1068
BciVI GTATCC 1 cut(s) 1799
BclI TGATCA 1 cut(s) 2019
BcoDI GTCTC 8 cut(s) 59, 1059, 1095, 1175, 1319, 1439, 1620, 1769
BfaI CTAG 1 cut(s) 920
BfmI CTRYAG 1 cut(s) 1516
BfoI RGCGCY 1 cut(s) 753
BfuI GTATCC 1 cut(s) 1799
BglII AGATCT 1 cut(s) 971
Bme1390I CCNGG 2 cut(s) 445, 1068
BmeT110I CYCGRG 1 cut(s) 1397
BmgT120I GGNCC 2 cut(s) 167, 292
BmiI GGNNCC 3 cut(s) 327, 344, 668
BmrFI CCNGG 2 cut(s) 445, 1068
BmsI GCATC 4 cut(s) 572, 996, 1577, 1923
BpiI GAAGAC 4 cut(s) 80, 304, 1104, 1526
BplI GAGNNNNNCTC 2 cut(s) 439, 471
BpmI CTGGAG 3 cut(s) 466, 1649, 2019
Bpu10I CCTNAGC 1 cut(s) 696
BpuEI CTTGAG 5 cut(s) 167, 1232, 1301, 1646, 1667
Bsa29I ATCGAT 1 cut(s) 1755
BsaAI YACGTR 1 cut(s) 1981
BsaBI GATNNNNATC 1 cut(s) 1914
BsaI GGTCTC 3 cut(s) 59, 1095, 1175
BsaJI CCNNGG 3 cut(s) 670, 1264, 1791
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 947
Bse1I ACTGG 2 cut(s) 843, 1492
Bse8I GATNNNNATC 1 cut(s) 1914
BseBI CCWGG 2 cut(s) 445, 1068
BseCI ATCGAT 1 cut(s) 1755
BseDI CCNNGG 3 cut(s) 670, 1264, 1791
BseGI GGATG 7 cut(s) 139, 259, 847, 1011, 1238, 1592, 1636
BseJI GATNNNNATC 1 cut(s) 1914
BseLI CCNNNNNNNGG 1 cut(s) 947
BseMII CTCAG 2 cut(s) 710, 1758
BseNI ACTGG 2 cut(s) 843, 1492
BseRI GAGGAG 3 cut(s) 206, 1652, 1670
BseYI CCCAGC 3 cut(s) 289, 483, 1032
BsgI GTGCAG 3 cut(s) 84, 1924, 1967
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 4 cut(s) 169, 294, 470, 1491
BshNI GGYRCC 1 cut(s) 342
BshVI ATCGAT 1 cut(s) 1755
BsiHKAI GWGCWC 2 cut(s) 1678, 1959
BsiHKCI CYCGRG 1 cut(s) 1397
BslI CCNNNNNNNGG 1 cut(s) 947
BsmAI GTCTC 8 cut(s) 59, 1059, 1095, 1175, 1319, 1439, 1620, 1769
BsmI GAATGC 3 cut(s) 1200, 1656, 1844
BsnI GGCC 4 cut(s) 169, 294, 470, 1491
Bso31I GGTCTC 3 cut(s) 59, 1095, 1175
BsoBI CYCGRG 1 cut(s) 1397
Bsp1286I GDGCHC 2 cut(s) 1678, 1959
Bsp143I GATC 8 cut(s) 325, 613, 666, 901, 971, 1707, 1756, 2019
Bsp19I CCATGG 1 cut(s) 670
BspACI CCGC 8 cut(s) 70, 340, 604, 679, 1772, 1842, 1970, 2026
BspANI GGCC 4 cut(s) 169, 294, 470, 1491
BspCNI CTCAG 2 cut(s) 709, 1759
BspDI ATCGAT 1 cut(s) 1755
BspFNI CGCG 1 cut(s) 72
BspHI TCATGA 1 cut(s) 1093
BspLI GGNNCC 3 cut(s) 327, 344, 668
BspMAI CTGCAG 1 cut(s) 1520
BspPI GGATC 7 cut(s) 320, 333, 661, 674, 909, 1715, 1751
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 3 cut(s) 59, 1095, 1175
BsrI ACTGG 2 cut(s) 843, 1492
BssECI CCNNGG 3 cut(s) 670, 1264, 1791
BssMI GATC 8 cut(s) 325, 613, 666, 901, 971, 1707, 1756, 2019
BssSI CACGAG 1 cut(s) 716
BssT1I CCWWGG 3 cut(s) 670, 1264, 1791
Bst2BI CACGAG 1 cut(s) 716
Bst2UI CCWGG 2 cut(s) 445, 1068
Bst4CI ACNGT 5 cut(s) 1002, 1053, 1673, 1945, 1961
Bst6I CTCTTC 1 cut(s) 1923
BstAPI GCANNNNNTGC 1 cut(s) 1015
BstBAI YACGTR 1 cut(s) 1981
BstC8I GCNNGC 3 cut(s) 677, 877, 932
BstDEI CTNAG 4 cut(s) 47, 696, 1508, 1767
BstDSI CCRYGG 1 cut(s) 670
BstEII GGTNACC 1 cut(s) 1747
BstF5I GGATG 7 cut(s) 139, 259, 847, 1011, 1238, 1592, 1636
BstFNI CGCG 1 cut(s) 72
BstH2I RGCGCY 1 cut(s) 753
BstHHI GCGC 3 cut(s) 752, 1938, 1978
BstKTI GATC 8 cut(s) 328, 616, 669, 904, 974, 1710, 1759, 2022
BstMAI GTCTC 8 cut(s) 59, 1059, 1095, 1175, 1319, 1439, 1620, 1769
BstMBI GATC 8 cut(s) 325, 613, 666, 901, 971, 1707, 1756, 2019
BstMWI GCNNNNNNNGC 6 cut(s) 449, 681, 1015, 1829, 1954, 1973
BstNI CCWGG 2 cut(s) 445, 1068
BstNSI RCATGY 1 cut(s) 679
BstPI GGTNACC 1 cut(s) 1747
BstSCI CCNGG 2 cut(s) 443, 1066
BstSFI CTRYAG 1 cut(s) 1516
BstUI CGCG 1 cut(s) 72
BstV2I GAAGAC 4 cut(s) 80, 304, 1104, 1526
BstX2I RGATCY 4 cut(s) 325, 666, 971, 1707
BstYI RGATCY 4 cut(s) 325, 666, 971, 1707
Bsu15I ATCGAT 1 cut(s) 1755
BsuI GTATCC 1 cut(s) 1799
BsuRI GGCC 4 cut(s) 169, 294, 470, 1491
BsuTUI ATCGAT 1 cut(s) 1755
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 7 cut(s) 139, 259, 847, 1011, 1238, 1592, 1636
BtsIMutI CAGTG 2 cut(s) 1678, 1957
Cac8I GCNNGC 3 cut(s) 677, 877, 932
CciI TCATGA 1 cut(s) 1093
CfoI GCGC 3 cut(s) 752, 1938, 1978
Cfr13I GGNCC 2 cut(s) 167, 292
ClaI ATCGAT 1 cut(s) 1755
CviAII CATG 8 cut(s) 243, 280, 404, 671, 676, 1010, 1094, 1952
DdeI CTNAG 4 cut(s) 47, 696, 1508, 1767
DpnI GATC 8 cut(s) 327, 615, 668, 903, 973, 1709, 1758, 2021
DpnII GATC 8 cut(s) 325, 613, 666, 901, 971, 1707, 1756, 2019
DraI TTTAAA 1 cut(s) 513
DrdI GACNNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 1923
EarI CTCTTC 1 cut(s) 1923
EciI GGCGGA 1 cut(s) 1787
Eco130I CCWWGG 3 cut(s) 670, 1264, 1791
Eco31I GGTCTC 3 cut(s) 59, 1095, 1175
Eco47III AGCGCT 1 cut(s) 751
Eco57I CTGAAG 2 cut(s) 1136, 1703
Eco72I CACGTG 1 cut(s) 1981
Eco88I CYCGRG 1 cut(s) 1397
Eco91I GGTNACC 1 cut(s) 1747
EcoO65I GGTNACC 1 cut(s) 1747
EcoRII CCWGG 2 cut(s) 443, 1066
EcoT14I CCWWGG 3 cut(s) 670, 1264, 1791
EcoT22I ATGCAT 3 cut(s) 281, 565, 1011
ErhI CCWWGG 3 cut(s) 670, 1264, 1791
FaeI CATG 8 cut(s) 246, 283, 407, 674, 679, 1013, 1097, 1955
FatI CATG 8 cut(s) 242, 279, 403, 670, 675, 1009, 1093, 1951
FauI CCCGC 3 cut(s) 77, 611, 672
FauNDI CATATG 1 cut(s) 632
FbaI TGATCA 1 cut(s) 2019
FokI GGATG 7 cut(s) 146, 266, 854, 1018, 1245, 1599, 1623
FspBI CTAG 1 cut(s) 920
GlaI GCGC 3 cut(s) 751, 1937, 1977
GsaI CCCAGC 3 cut(s) 293, 487, 1036
GsuI CTGGAG 3 cut(s) 466, 1649, 2019
HaeII RGCGCY 1 cut(s) 753
HaeIII GGCC 4 cut(s) 169, 294, 470, 1491
HhaI GCGC 3 cut(s) 752, 1938, 1978
Hin1II CATG 8 cut(s) 246, 283, 407, 674, 679, 1013, 1097, 1955
Hin6I GCGC 3 cut(s) 750, 1936, 1976
HinP1I GCGC 3 cut(s) 750, 1936, 1976
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 1 cut(s) 1280
HphI GGTGA 2 cut(s) 115, 213
Hpy166II GTNNAC 4 cut(s) 81, 490, 577, 638
Hpy188I TCNGA 8 cut(s) 111, 334, 459, 822, 1206, 1722, 1768, 1900
Hpy8I GTNNAC 4 cut(s) 81, 490, 577, 638
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 3 cut(s) 339, 348, 1223
HpyCH4III ACNGT 5 cut(s) 1002, 1053, 1673, 1945, 1961
HpyCH4IV ACGT 3 cut(s) 1075, 1420, 1980
HpyF10VI GCNNNNNNNGC 6 cut(s) 449, 681, 1015, 1829, 1954, 1973
HpyF3I CTNAG 4 cut(s) 47, 696, 1508, 1767
HpySE526I ACGT 3 cut(s) 1075, 1420, 1980
Hsp92II CATG 8 cut(s) 246, 283, 407, 674, 679, 1013, 1097, 1955
HspAI GCGC 3 cut(s) 750, 1936, 1976
Ksp22I TGATCA 1 cut(s) 2019
Kzo9I GATC 8 cut(s) 325, 613, 666, 901, 971, 1707, 1756, 2019
LmnI GCTCC 1 cut(s) 1733
LweI GCATC 4 cut(s) 572, 996, 1577, 1923
MaeI CTAG 1 cut(s) 920
MaeII ACGT 3 cut(s) 1075, 1420, 1980
MaeIII GTNAC 4 cut(s) 121, 907, 1747, 1854
MalI GATC 8 cut(s) 327, 615, 668, 903, 973, 1709, 1758, 2021
MboI GATC 8 cut(s) 325, 613, 666, 901, 971, 1707, 1756, 2019
MfeI CAATTG 2 cut(s) 186, 1473
MflI RGATCY 4 cut(s) 325, 666, 971, 1707
MhlI GDGCHC 2 cut(s) 1678, 1959
MlsI TGGCCA 1 cut(s) 470
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 5 cut(s) 83, 203, 1260, 1330, 1844
MmeI TCCRAC 2 cut(s) 1014, 1387
Mox20I TGGCCA 1 cut(s) 470
Mph1103I ATGCAT 3 cut(s) 281, 565, 1011
MroXI GAANNNNTTC 3 cut(s) 598, 1041, 2009
MscI TGGCCA 1 cut(s) 470
MslI CAYNNNNRTG 3 cut(s) 128, 593, 643
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 2 cut(s) 1886, 1970
MspR9I CCNGG 2 cut(s) 445, 1068
MunI CAATTG 2 cut(s) 186, 1473
Mva1269I GAATGC 3 cut(s) 1200, 1656, 1844
MvaI CCWGG 2 cut(s) 445, 1068
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 6 cut(s) 449, 681, 1015, 1829, 1954, 1973
NcoI CCATGG 1 cut(s) 670
NdeI CATATG 1 cut(s) 632
NdeII GATC 8 cut(s) 325, 613, 666, 901, 971, 1707, 1756, 2019
NlaIII CATG 8 cut(s) 246, 283, 407, 674, 679, 1013, 1097, 1955
NlaIV GGNNCC 3 cut(s) 327, 344, 668
NmeAIII GCCGAG 1 cut(s) 1578
NmuCI GTSAC 3 cut(s) 121, 907, 1854
NsiI ATGCAT 3 cut(s) 281, 565, 1011
NspI RCATGY 1 cut(s) 679
PaeI GCATGC 1 cut(s) 679
PagI TCATGA 1 cut(s) 1093
PctI GAATGC 3 cut(s) 1200, 1656, 1844
PdmI GAANNNNTTC 3 cut(s) 598, 1041, 2009
PfeI GAWTC 6 cut(s) 599, 1207, 1294, 1346, 1393, 1504
PleI GAGTC 5 cut(s) 82, 202, 1259, 1329, 1844
PmaCI CACGTG 1 cut(s) 1981
PmlI CACGTG 1 cut(s) 1981
PpsI GAGTC 5 cut(s) 82, 202, 1259, 1329, 1844
Ppu21I YACGTR 1 cut(s) 1981
PshBI ATTAAT 1 cut(s) 1361
Psp1406I AACGTT 1 cut(s) 1420
Psp6I CCWGG 2 cut(s) 443, 1066
PspCI CACGTG 1 cut(s) 1981
PspEI GGTNACC 1 cut(s) 1747
PspFI CCCAGC 3 cut(s) 289, 483, 1032
PspGI CCWGG 2 cut(s) 443, 1066
PspN4I GGNNCC 3 cut(s) 327, 344, 668
PspPI GGNCC 2 cut(s) 167, 292
PstI CTGCAG 1 cut(s) 1520
PsuI RGATCY 4 cut(s) 325, 666, 971, 1707
PvuII CAGCTG 1 cut(s) 1886
RseI CAYNNNNRTG 3 cut(s) 128, 593, 643
Sau3AI GATC 8 cut(s) 325, 613, 666, 901, 971, 1707, 1756, 2019
Sau96I GGNCC 2 cut(s) 167, 292
SchI GAGTC 5 cut(s) 83, 203, 1260, 1330, 1844
ScrFI CCNGG 2 cut(s) 445, 1068
SduI GDGCHC 2 cut(s) 1678, 1959
SfaNI GCATC 4 cut(s) 572, 996, 1577, 1923
SfcI CTRYAG 1 cut(s) 1516
SmiMI CAYNNNNRTG 3 cut(s) 128, 593, 643
SmlI CTYRAG 5 cut(s) 146, 1211, 1316, 1625, 1682
SmoI CTYRAG 5 cut(s) 146, 1211, 1316, 1625, 1682
SphI GCATGC 1 cut(s) 679
SsiI CCGC 8 cut(s) 70, 340, 604, 679, 1772, 1842, 1970, 2026
SspI AATATT 1 cut(s) 861
SspMI CTAG 1 cut(s) 920
StyD4I CCNGG 2 cut(s) 443, 1066
StyI CCWWGG 3 cut(s) 670, 1264, 1791
TaaI ACNGT 5 cut(s) 1002, 1053, 1673, 1945, 1961
TaiI ACGT 3 cut(s) 1078, 1423, 1983
TaqI TCGA 3 cut(s) 1134, 1740, 1755
TauI GCSGC 3 cut(s) 343, 1973, 2029
TfiI GAWTC 6 cut(s) 599, 1207, 1294, 1346, 1393, 1504
TscAI CASTG 2 cut(s) 1678, 1964
TseFI GTSAC 3 cut(s) 121, 907, 1854
Tsp45I GTSAC 3 cut(s) 121, 907, 1854
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 2 cut(s) 1678, 1964
VspI ATTAAT 1 cut(s) 1361
XapI RAATTY 3 cut(s) 1129, 1240, 1893
XceI RCATGY 1 cut(s) 679
XcmI CCANNNNNNNNNTGG 1 cut(s) 1262
XmnI GAANNNNTTC 3 cut(s) 598, 1041, 2009
XspI CTAG 1 cut(s) 920
Zsp2I ATGCAT 3 cut(s) 281, 565, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.