RLG00000030369

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000105
Physical Location & Seq
Forward (+)
60189426 .. 60197537
8112 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030369

Sequence Viewer

Length: 3222 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAGTACGATGTGTTTCTAAGTTTCAGGGGGAAAGATACCCGCTGGTTTTTTACCGACCGTCTGTATTCTGCACTAAATCAAAGAGGAATTCACACCTTTATGGATGACGCAGAACTTGAGAAAGGAAAAACCATTAGGCCTGAACTTTTAGCTGCAATTGAGCACTCTAGATGTGCGATTGTCATTCTCTCAGAGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATGGACCAACAAGTCCTCCCCGTCTTCTTAAACGGCGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGTTTTGAGTTCAAAAATAAACATGAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGGATTAATGAGGACAGACTAAATGCGTGGAGAGCTGCTTTGACTGAGGTCGCCAATCTCTCGGGCTGGCATTCCAAGAATATATTCGAATCAAAACTAATTCAGGATATTGTTAATTATATACTAAAGAAGTCTGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATCAATTCTCGCATTGATGATTTATTAAGCAATTACATATATCCTCAGCTTGGTGGGGCGGTGCGTTTAATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCCCGAGCTATCTATAATGAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAAAAATGGCCTAATTTCTGTACAAGAAAAACTTCTTTCCACAATCCTGATGGCAAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGCTGTGTATAATAAAGGTGCTTGTTGTTATTGATGATGTGGATGAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGACTAGAATTATCATAACCACCACAGATAATCAGTTGTTGAAGGCACATGATGTTGATGCAACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAGGGCTTTTAAGAAATGTCCCCCACCAGAACATTATTTGCAACTGTGCTACCATATTTTAGGGTATGCTCAGGGGCTTCCATTGGCTCTCGTGGTTTTAGGTTCTTTTCTGTTTGGTAGAAGCACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCCCATAAACATATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCGAACAACGAACTGTTGATGCATGATTTGCTACAAGAAACGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGAGATCAACGATGTGCTGACGAGAAATACGTCTACTTGTTTATCTCTATTATGCAAATTGTCTAATTTCGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACTTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTTTCCTGAATCTTAAAGACTGCAAAAGTCTCAAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTGATTCTTTCTACCTGCTCAAAACTTAAGAAGATTCCCAAGTTTGTTGGAAATATGGAACGTTTGTCGGTGCTTTATCTTGATGGGACTGCCATTAAGGAACTGCCTGTTTCAATCGAACAGCTGAGCGGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTTTGTCTCCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCCGGTAATGGTGGAGATGGACTGTTTTGAGGAAACTGATGTGAATAATGGGTCTGCAATGGAATCATCCGCTACACATGATCTAATAAGATATGTGAGAGGTTCAATCTTTCATGGATGCGAGTTAATTTGTGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGATGCAAAAAGTGCTGCAGAAAGTGAATAATGAGCCAATGAGTTTCCACTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAACTGCAATCTTGGTGAAGAAGCGTTTGCCAACGAATTTGGTCACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTTCCAGCATAGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGCATACTGGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAGTAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGCTCTTGAAAGTAGTCCTCCGGAGCAACATTCTCTTCCAGAACAGTGCTATCCTCAAGGACGGCAACTAGTTGACTCGATGCCATTGGCCCTTTTCGATAAGAATCTCTATCCTCCACACCACGTAACCATCTATTGTACATCTGAGGCAGAGACAAAACTTATGTCCTCGGTTAAAATGGGGTACACCGAATTGGAGGCTTCAATGGCAATAGAGAGATGCGCTGCTCAAATTTCAAAGGCAGAAGATGGCCATCTTCCCCTTGAAGAGATGGTATTCGTACACATCTGTGGAGTGAGTCCTGAGAAGAATAAAAGGAAACTTTGTGAATCTTCATTGCTGAAAAGGAAAAGTATTTCGGGACATGGAAACCAGATCATAGGGGAAGATGCTGGTGCAATTCATCTCCCAAATCCAATGAATGGGTTTGGGACTCCAGCTGAACTCTGTCAAATCCACAGAAGTCTTCCAAAGGCAGCCACAGGACCTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1074

Amino Acids

120.72

Weight (kDa)

6.36

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 189 1.5e-48 TIR domain
TIR_2 PF13676 14 - 125 4.6e-18 TIR domain
NB-ARC PF00931 217 - 370 1.1e-17 NB-ARC domain
NACHT PF05729 219 - 339 1.9e-06 NACHT domain
WHD_ROQ1 PF23282 437 - 508 4.2e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 616 - 700 4.1e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 627 - 696 4.1e-09 Leucine-rich repeat region
LRR_14 PF23598 777 - 859 9.7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1688
AasI GACNNNNNNGTC 1 cut(s) 299
Acc36I ACCTGC 3 cut(s) 752, 1688, 1883
AccB1I GGYRCC 1 cut(s) 345
AccB7I CCANNNNNTGG 1 cut(s) 3149
AccBSI CCGCTC 1 cut(s) 1989
AccI GTMKAC 2 cut(s) 554, 1598
AccIII TCCGGA 1 cut(s) 2746
AciI CCGC 7 cut(s) 70, 343, 647, 676, 883, 1989, 2178
AclI AACGTT 1 cut(s) 1921
AclWI GGATC 6 cut(s) 323, 336, 658, 671, 1377, 2251
AcoI YGGCCR 1 cut(s) 2977
AcsI RAATTY 5 cut(s) 117, 720, 2402, 2614, 2958
AcuI CTGAAG 1 cut(s) 2729
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 35, 798, 2584, 2866, 2912, 3009
AfiI CCNNNNNNNGG 4 cut(s) 638, 1415, 2333, 3149
AflII CTTAAG 1 cut(s) 1886
AhlI ACTAGT 1 cut(s) 2794
AjnI CCWGG 2 cut(s) 976, 1534
AjuI GAANNNNNNNTTGG 4 cut(s) 482, 514, 2489, 2521
AleI CACNNNNGTG 1 cut(s) 3015
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 8 cut(s) 1527, 1563, 1676, 1709, 1820, 2039, 2515, 2873
AlwI GGATC 6 cut(s) 323, 336, 658, 671, 1377, 2251
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 2 cut(s) 475, 702
Aor13HI TCCGGA 1 cut(s) 2746
AoxI GGCC 5 cut(s) 167, 784, 1990, 2814, 2977
ApeKI GCWGC 6 cut(s) 182, 449, 866, 2287, 2951, 3203
ApoI RAATTY 5 cut(s) 117, 720, 2402, 2614, 2958
ArsI GACNNNNNNTTYG 2 cut(s) 2876, 2908
AseI ATTAAT 1 cut(s) 420
Asp700I GAANNNNTTC 6 cut(s) 497, 592, 807, 1077, 1509, 2714
AspLEI GCGC 1 cut(s) 2951
AspS9I GGNCC 4 cut(s) 292, 974, 2815, 3212
AsuHPI GGTGA 2 cut(s) 2393, 2437
AsuII TTCGAA 1 cut(s) 501
AvaI CYCGRG 2 cut(s) 475, 702
AvaII GGWCC 3 cut(s) 292, 974, 3212
BalI TGGCCA 1 cut(s) 2979
BamHI GGATCC 2 cut(s) 328, 663
BanI GGYRCC 1 cut(s) 345
BauI CACGAG 1 cut(s) 1184
BbsI GAAGAC 4 cut(s) 304, 2453, 2717, 3185
Bbv12I GWGCWC 1 cut(s) 195
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 6 cut(s) 169, 436, 853, 2274, 2938, 3215
BceAI ACGGC 2 cut(s) 337, 2804
BciT130I CCWGG 2 cut(s) 978, 1536
BcoDI GTCTC 8 cut(s) 1527, 1563, 1676, 1709, 1820, 2039, 2515, 2873
BcuI ACTAGT 1 cut(s) 2794
BfaI CTAG 4 cut(s) 198, 984, 1388, 2795
BfmI CTRYAG 2 cut(s) 2017, 2288
BfrI CTTAAG 1 cut(s) 1886
BfuAI ACCTGC 3 cut(s) 752, 1688, 1883
BglII AGATCT 2 cut(s) 1439, 1723
BisI GCNGC 9 cut(s) 183, 344, 450, 867, 884, 1990, 2288, 2952, 3204
BlpI GCTNAGC 1 cut(s) 1985
BlsI GCNGC 9 cut(s) 184, 345, 451, 868, 885, 1991, 2289, 2953, 3205
Bme1390I CCNGG 2 cut(s) 978, 1536
Bme18I GGWCC 3 cut(s) 292, 974, 3212
BmeT110I CYCGRG 2 cut(s) 475, 702
BmgT120I GGNCC 4 cut(s) 292, 974, 2815, 3212
BmiI GGNNCC 3 cut(s) 330, 347, 665
BmrFI CCNGG 2 cut(s) 978, 1536
BoxI GACNNNNGTC 1 cut(s) 461
BpiI GAAGAC 4 cut(s) 304, 2453, 2717, 3185
BpmI CTGGAG 1 cut(s) 3147
Bpu10I CCTNAGC 2 cut(s) 633, 1164
Bpu1102I GCTNAGC 1 cut(s) 1985
Bpu14I TTCGAA 1 cut(s) 501
BpuEI CTTGAG 5 cut(s) 167, 1733, 1802, 2513, 2766
BsaAI YACGTR 1 cut(s) 2851
BsaBI GATNNNNATC 3 cut(s) 1368, 2829, 2979
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1563, 1676
BsaJI CCNNGG 4 cut(s) 667, 977, 1765, 2895
BsaWI WCCGGW 2 cut(s) 2110, 2746
BsaXI ACNNNNNCTCC 3 cut(s) 288, 318, 3199
Bsc4I CCNNNNNNNGG 4 cut(s) 638, 1415, 2333, 3149
Bse1I ACTGG 3 cut(s) 971, 1311, 2565
Bse3DI GCAATG 2 cut(s) 2172, 3062
Bse8I GATNNNNATC 3 cut(s) 1368, 2829, 2979
BseAI TCCGGA 1 cut(s) 2746
BseBI CCWGG 2 cut(s) 978, 1536
BseDI CCNNGG 4 cut(s) 667, 977, 1765, 2895
BseJI GATNNNNATC 3 cut(s) 1368, 2829, 2979
BseLI CCNNNNNNNGG 4 cut(s) 638, 1415, 2333, 3149
BseMI GCAATG 2 cut(s) 2172, 3062
BseMII CTCAG 8 cut(s) 234, 450, 647, 1178, 1976, 2354, 2862, 3021
BseNI ACTGG 3 cut(s) 971, 1311, 2565
BseXI GCAGC 6 cut(s) 169, 436, 853, 2274, 2938, 3215
BseYI CCCAGC 1 cut(s) 1500
BsgI GTGCAG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 5 cut(s) 169, 786, 1992, 2816, 2979
BshNI GGYRCC 1 cut(s) 345
BsiEI CGRYCG 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 2 cut(s) 475, 702
BsiSI CCGG 2 cut(s) 2111, 2747
BslFI GGGAC 5 cut(s) 994, 1098, 1960, 3102, 3172
BslI CCNNNNNNNGG 4 cut(s) 638, 1415, 2333, 3149
BsmAI GTCTC 8 cut(s) 1527, 1563, 1676, 1709, 1820, 2039, 2515, 2873
BsmFI GGGAC 5 cut(s) 994, 1098, 1960, 3102, 3172
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 5 cut(s) 169, 786, 1992, 2816, 2979
Bso31I GGTCTC 2 cut(s) 1563, 1676
BsoBI CYCGRG 2 cut(s) 475, 702
Bsp119I TTCGAA 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 195
Bsp13I TCCGGA 1 cut(s) 2746
Bsp1407I TGTACA 2 cut(s) 796, 2864
Bsp143I GATC 9 cut(s) 328, 663, 1369, 1439, 1569, 1723, 2188, 2243, 3102
Bsp1720I GCTNAGC 1 cut(s) 1985
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 7 cut(s) 70, 343, 647, 676, 883, 1989, 2178
BspANI GGCC 5 cut(s) 169, 786, 1992, 2816, 2979
BspCNI CTCAG 8 cut(s) 233, 451, 646, 1177, 1977, 2355, 2863, 3022
BspEI TCCGGA 1 cut(s) 2746
BspHI TCATGA 1 cut(s) 1561
BspLI GGNNCC 3 cut(s) 330, 347, 665
BspMAI CTGCAG 2 cut(s) 2021, 2292
BspMI ACCTGC 3 cut(s) 752, 1688, 1883
BspPI GGATC 6 cut(s) 323, 336, 658, 671, 1377, 2251
BspT104I TTCGAA 1 cut(s) 501
BspT107I GGYRCC 1 cut(s) 345
BspTI CTTAAG 1 cut(s) 1886
BspTNI GGTCTC 2 cut(s) 1563, 1676
BsrBI CCGCTC 1 cut(s) 1989
BsrDI GCAATG 2 cut(s) 2172, 3062
BsrGI TGTACA 2 cut(s) 796, 2864
BsrI ACTGG 3 cut(s) 971, 1311, 2565
BssECI CCNNGG 4 cut(s) 667, 977, 1765, 2895
BssMI GATC 9 cut(s) 328, 663, 1369, 1439, 1569, 1723, 2188, 2243, 3102
BssNAI GTATAC 1 cut(s) 555
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1184
BssT1I CCWWGG 2 cut(s) 667, 1765
Bst1107I GTATAC 1 cut(s) 555
Bst2BI CACGAG 1 cut(s) 1184
Bst2UI CCWGG 2 cut(s) 978, 1536
Bst4CI ACNGT 7 cut(s) 89, 1067, 1140, 1470, 1521, 2132, 2772
Bst6I CTCTTC 4 cut(s) 19, 1560, 2766, 2988
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1886
BstAPI GCANNNNNTGC 2 cut(s) 1483, 2284
BstAUI TGTACA 2 cut(s) 796, 2864
BstBAI YACGTR 1 cut(s) 2851
BstBI TTCGAA 1 cut(s) 501
BstC8I GCNNGC 4 cut(s) 482, 674, 1345, 1400
BstDSI CCRYGG 1 cut(s) 667
BstEII GGTNACC 1 cut(s) 2425
BstHHI GCGC 1 cut(s) 2951
BstKTI GATC 9 cut(s) 331, 666, 1372, 1442, 1572, 1726, 2191, 2246, 3105
BstMAI GTCTC 8 cut(s) 1527, 1563, 1676, 1709, 1820, 2039, 2515, 2873
BstMBI GATC 9 cut(s) 328, 663, 1369, 1439, 1569, 1723, 2188, 2243, 3102
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 6 cut(s) 446, 678, 1483, 2284, 2488, 2933
BstNI CCWGG 2 cut(s) 978, 1536
BstNSI RCATGY 1 cut(s) 676
BstPAI GACNNNNGTC 1 cut(s) 461
BstPI GGTNACC 1 cut(s) 2425
BstSCI CCNGG 2 cut(s) 976, 1534
BstSFI CTRYAG 2 cut(s) 2017, 2288
BstV1I GCAGC 6 cut(s) 169, 436, 853, 2274, 2938, 3215
BstV2I GAAGAC 4 cut(s) 304, 2453, 2717, 3185
BstX2I RGATCY 5 cut(s) 328, 663, 1439, 1723, 2243
BstYI RGATCY 5 cut(s) 328, 663, 1439, 1723, 2243
BstZ17I GTATAC 1 cut(s) 555
BsuRI GGCC 5 cut(s) 169, 786, 1992, 2816, 2979
BtgI CCRYGG 1 cut(s) 667
BtsIMutI CAGTG 3 cut(s) 1218, 2588, 2777
BveI ACCTGC 3 cut(s) 752, 1688, 1883
Cac8I GCNNGC 4 cut(s) 482, 674, 1345, 1400
CaiI CAGNNNCTG 1 cut(s) 866
CciI TCATGA 1 cut(s) 1561
CfoI GCGC 1 cut(s) 2951
Cfr13I GGNCC 4 cut(s) 292, 974, 2815, 3212
CseI GACGC 1 cut(s) 146
Csp6I GTAC 6 cut(s) 34, 797, 2583, 2865, 2911, 3008
CviQI GTAC 6 cut(s) 34, 797, 2583, 2865, 2911, 3008
DpnI GATC 9 cut(s) 330, 665, 1371, 1441, 1571, 1725, 2190, 2245, 3104
DpnII GATC 9 cut(s) 328, 663, 1369, 1439, 1569, 1723, 2188, 2243, 3102
DraI TTTAAA 1 cut(s) 2250
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 1 cut(s) 2977
Eam1104I CTCTTC 4 cut(s) 19, 1560, 2766, 2988
EarI CTCTTC 4 cut(s) 19, 1560, 2766, 2988
Eco130I CCWWGG 2 cut(s) 667, 1765
Eco147I AGGCCT 1 cut(s) 169
Eco31I GGTCTC 2 cut(s) 1563, 1676
Eco47I GGWCC 3 cut(s) 292, 974, 3212
Eco57I CTGAAG 1 cut(s) 2729
Eco88I CYCGRG 2 cut(s) 475, 702
Eco91I GGTNACC 1 cut(s) 2425
EcoO109I RGGNCCY 1 cut(s) 3212
EcoO65I GGTNACC 1 cut(s) 2425
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 2 cut(s) 976, 1534
EcoT14I CCWWGG 2 cut(s) 667, 1765
EcoT22I ATGCAT 3 cut(s) 281, 1479, 2611
ErhI CCWWGG 2 cut(s) 667, 1765
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 5 cut(s) 994, 1098, 1960, 3102, 3172
FauI CCCGC 2 cut(s) 77, 669
FblI GTMKAC 2 cut(s) 554, 1598
Fnu4HI GCNGC 9 cut(s) 183, 344, 450, 867, 884, 1990, 2288, 2952, 3204
Fsp4HI GCNGC 9 cut(s) 183, 344, 450, 867, 884, 1990, 2288, 2952, 3204
FspBI CTAG 4 cut(s) 198, 984, 1388, 2795
GlaI GCGC 1 cut(s) 2950
GluI GCNGC 9 cut(s) 183, 344, 450, 867, 884, 1990, 2288, 2952, 3204
GsaI CCCAGC 1 cut(s) 1504
GsuI CTGGAG 1 cut(s) 3147
HaeIII GGCC 5 cut(s) 169, 786, 1992, 2816, 2979
HapII CCGG 2 cut(s) 2111, 2747
HgaI GACGC 1 cut(s) 146
HhaI GCGC 1 cut(s) 2951
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 2949
HinP1I GCGC 1 cut(s) 2949
HincII GTYRAC 4 cut(s) 9, 571, 933, 2800
HindII GTYRAC 4 cut(s) 9, 571, 933, 2800
HindIII AAGCTT 2 cut(s) 1781, 2489
HpaII CCGG 2 cut(s) 2111, 2747
HphI GGTGA 2 cut(s) 2393, 2437
Hpy166II GTNNAC 9 cut(s) 9, 555, 571, 933, 1517, 1599, 2800, 2913, 3010
Hpy188I TCNGA 7 cut(s) 223, 337, 1290, 1707, 2358, 2539, 2872
Hpy8I GTNNAC 9 cut(s) 9, 555, 571, 933, 1517, 1599, 2800, 2913, 3010
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 7 cut(s) 342, 1015, 1354, 1724, 2274, 2475, 2552
HpyCH4III ACNGT 7 cut(s) 89, 1067, 1140, 1470, 1521, 2132, 2772
HpyCH4IV ACGT 4 cut(s) 1543, 1595, 1921, 2850
HpyF10VI GCNNNNNNNGC 6 cut(s) 446, 678, 1483, 2284, 2488, 2933
HpySE526I ACGT 4 cut(s) 1543, 1595, 1921, 2850
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 2949
Kpn2I TCCGGA 1 cut(s) 2746
Kzo9I GATC 9 cut(s) 328, 663, 1369, 1439, 1569, 1723, 2188, 2243, 3102
LmnI GCTCC 2 cut(s) 863, 2749
Lsp1109I GCAGC 6 cut(s) 169, 436, 853, 2274, 2938, 3215
MaeI CTAG 4 cut(s) 198, 984, 1388, 2795
MaeII ACGT 4 cut(s) 1543, 1595, 1921, 2850
MaeIII GTNAC 6 cut(s) 1375, 2366, 2408, 2425, 2687, 2851
MalI GATC 9 cut(s) 330, 665, 1371, 1441, 1571, 1725, 2190, 2245, 3104
MbiI CCGCTC 1 cut(s) 1989
MboI GATC 9 cut(s) 328, 663, 1369, 1439, 1569, 1723, 2188, 2243, 3102
MfeI CAATTG 2 cut(s) 186, 2653
MflI RGATCY 5 cut(s) 328, 663, 1439, 1723, 2243
MhlI GDGCHC 1 cut(s) 195
MlsI TGGCCA 1 cut(s) 2979
MluNI TGGCCA 1 cut(s) 2979
MlyI GAGTC 4 cut(s) 1831, 2795, 3034, 3154
MmeI TCCRAC 1 cut(s) 1888
Mox20I TGGCCA 1 cut(s) 2979
Mph1103I ATGCAT 3 cut(s) 281, 1479, 2611
MroI TCCGGA 1 cut(s) 2746
MroXI GAANNNNTTC 6 cut(s) 497, 592, 807, 1077, 1509, 2714
MscI TGGCCA 1 cut(s) 2979
MslI CAYNNNNRTG 2 cut(s) 128, 3015
Msp20I TGGCCA 1 cut(s) 2979
MspA1I CMGCKG 3 cut(s) 72, 1984, 3167
MspCI CTTAAG 1 cut(s) 1886
MspI CCGG 2 cut(s) 2111, 2747
MspR9I CCNGG 2 cut(s) 978, 1536
MunI CAATTG 2 cut(s) 186, 2653
Mva1269I GAATGC 1 cut(s) 484
MvaI CCWGG 2 cut(s) 978, 1536
MwoI GCNNNNNNNGC 6 cut(s) 446, 678, 1483, 2284, 2488, 2933
NcoI CCATGG 1 cut(s) 667
NdeII GATC 9 cut(s) 328, 663, 1369, 1439, 1569, 1723, 2188, 2243, 3102
NlaIV GGNNCC 3 cut(s) 330, 347, 665
NmeAIII GCCGAG 1 cut(s) 2079
NmuCI GTSAC 3 cut(s) 1375, 2408, 2425
NsiI ATGCAT 3 cut(s) 281, 1479, 2611
NspI RCATGY 1 cut(s) 676
NspV TTCGAA 1 cut(s) 501
OliI CACNNNNGTG 1 cut(s) 3015
PaeI GCATGC 1 cut(s) 676
PagI TCATGA 1 cut(s) 1561
PaqCI CACCTGC 1 cut(s) 1688
PceI AGGCCT 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 1592
PctI GAATGC 1 cut(s) 484
PdmI GAANNNNTTC 6 cut(s) 497, 592, 807, 1077, 1509, 2714
PflMI CCANNNNNTGG 1 cut(s) 3149
PkrI GCNGC 9 cut(s) 184, 345, 451, 868, 885, 1991, 2289, 2953, 3205
PleI GAGTC 4 cut(s) 1830, 2795, 3033, 3154
PpsI GAGTC 4 cut(s) 1830, 2795, 3033, 3154
Ppu21I YACGTR 1 cut(s) 2851
PpuMI RGGWCCY 1 cut(s) 3212
PshAI GACNNNNGTC 1 cut(s) 461
PshBI ATTAAT 1 cut(s) 420
Psp1406I AACGTT 1 cut(s) 1921
Psp5II RGGWCCY 1 cut(s) 3212
Psp6I CCWGG 2 cut(s) 976, 1534
PspEI GGTNACC 1 cut(s) 2425
PspFI CCCAGC 1 cut(s) 1500
PspGI CCWGG 2 cut(s) 976, 1534
PspN4I GGNNCC 3 cut(s) 330, 347, 665
PspPI GGNCC 4 cut(s) 292, 974, 2815, 3212
PspPPI RGGWCCY 1 cut(s) 3212
PstI CTGCAG 2 cut(s) 2021, 2292
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 5 cut(s) 328, 663, 1439, 1723, 2243
PvuII CAGCTG 2 cut(s) 1984, 3167
RsaI GTAC 6 cut(s) 35, 798, 2584, 2866, 2912, 3009
RsaNI GTAC 6 cut(s) 34, 797, 2583, 2865, 2911, 3008
RseI CAYNNNNRTG 2 cut(s) 128, 3015
SatI GCNGC 9 cut(s) 183, 344, 450, 867, 884, 1990, 2288, 2952, 3204
Sau3AI GATC 9 cut(s) 328, 663, 1369, 1439, 1569, 1723, 2188, 2243, 3102
Sau96I GGNCC 4 cut(s) 292, 974, 2815, 3212
SchI GAGTC 4 cut(s) 1831, 2795, 3034, 3154
ScrFI CCNGG 2 cut(s) 978, 1536
SduI GDGCHC 1 cut(s) 195
SfcI CTRYAG 2 cut(s) 2017, 2288
SfuI TTCGAA 1 cut(s) 501
SinI GGWCC 3 cut(s) 292, 974, 3212
SmiMI CAYNNNNRTG 2 cut(s) 128, 3015
SmlI CTYRAG 6 cut(s) 146, 1712, 1817, 1886, 2492, 2781
SmoI CTYRAG 6 cut(s) 146, 1712, 1817, 1886, 2492, 2781
SpeI ACTAGT 1 cut(s) 2794
SphI GCATGC 1 cut(s) 676
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 7 cut(s) 70, 343, 647, 676, 883, 1989, 2178
SspI AATATT 1 cut(s) 1329
SspMI CTAG 4 cut(s) 198, 984, 1388, 2795
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 2 cut(s) 976, 1534
StyI CCWWGG 2 cut(s) 667, 1765
TaaI ACNGT 7 cut(s) 89, 1067, 1140, 1470, 1521, 2132, 2772
TaiI ACGT 4 cut(s) 1546, 1598, 1924, 2853
TaqI TCGA 6 cut(s) 501, 1457, 1635, 1977, 2804, 2823
TaqII GACCGA 1 cut(s) 753
TatI WGTACW 3 cut(s) 796, 2582, 2864
TauI GCSGC 3 cut(s) 346, 886, 1992
TscAI CASTG 3 cut(s) 1225, 2595, 2777
TseFI GTSAC 3 cut(s) 1375, 2408, 2425
TseI GCWGC 6 cut(s) 182, 449, 866, 2287, 2951, 3203
Tsp45I GTSAC 3 cut(s) 1375, 2408, 2425
TspRI CASTG 3 cut(s) 1225, 2595, 2777
Van91I CCANNNNNTGG 1 cut(s) 3149
Vha464I CTTAAG 1 cut(s) 1886
VpaK11BI GGWCC 3 cut(s) 292, 974, 3212
VspI ATTAAT 1 cut(s) 420
XapI RAATTY 5 cut(s) 117, 720, 2402, 2614, 2958
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 676
XcmI CCANNNNNNNNNTGG 2 cut(s) 823, 1763
XmiI GTMKAC 2 cut(s) 554, 1598
XmnI GAANNNNTTC 6 cut(s) 497, 592, 807, 1077, 1509, 2714
XspI CTAG 4 cut(s) 198, 984, 1388, 2795
Zsp2I ATGCAT 3 cut(s) 281, 1479, 2611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.