Rw1G004440

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
8466557 .. 8470813
4257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004440.1

Sequence Viewer

Length: 3624 bp
ATGGCCTCAACCTCTTCTCGTCGTCGATGGAAATACGATGTGTTTCTAAACTTCAGGGGGAAAGATACCCGCAAATATTTTACCAGTAATCTGTATGCTGAATTAAATCAGAAAGGAATTCTCTCCTTTTGGGATGGGGCAGAGCTTGAGAAAGGGAAATCCATTTGGCCTGAACTTTTAGCTGCAATTGAGGACTCTAGATTTGCGATTGTCATTCTCTCAGAGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATGGGTCAACAAGTCCTGCCCGTCTTCTACAAGGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGGTTTGAGCTCAAAAGGGACCCCGAAGTAGAAGTAAGGGCACATGAAGAAGTTTATGGGAATAATGAGGACAGACTAAATGCGTGGAGAGATGCTTTGACACAGGTGGCCAACCTTTCTGGCTGGGATTCCAAGAATTATCAACATGAATCAAGTTTAATTGACGAGCTTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGGTTCTCGCATTGATGATTTATTAAGCAATTACATATATCCGCAGCTTGATGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCCAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCTAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTCGTGTAGAATGAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCACAACTGGTTTGGTTCAGGGACTAGAATTATCATAACCACCACAGATTTTCAGTTGTTAAAGGCACACGGTGTTGATGCTACGTACAAGGCTAATGGCTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGCATCTGTGCTACGATATTACAGGGTATGCTAAGGGGCTTCCCTTGGCTGTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCGCTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTCTCATAACTCTCTCCAACAACGAGCTATGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGATGACATCAACAATGTGCTGAAGAAAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACGAGTGGCTCATTGGAAGCCAGAAGCCTTTACAAATTTGTCTGAACTTGGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCATTTGTCTTTCTAGTTCCTTGAGACTCCTTGAATGGACTGGGTATCCCTTAAGATCTCTCCCAAAATTTTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAAAATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTAAATCTTAAAGACTGCAAAAATCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGTCATTGAATCTAAACAACTGCAGAAAACTTGTTTGTCTTCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATCTGGGGGAGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCACCTACCAATGATCGCATAAAACATGTGAGAGTTTCAATCTTTCATCAATGCAAAGTAGTTTGGGGATCTTTAAATAAGTTTTTGCCTTCTGGATTGGTGCAAGAAGTGAATACAGAGCCAATGAGTTTCCGCTTGCCTATATCTCAAAAAGTGAATGCAAAGCCAACGAGTGTCCGCTTGCCTATTTCTGGTCTCTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTCGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCATTCCTTCAGGCGTTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGACTAAATAGATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATGAAGGATGCAATAACATAGCATTTGAAATGCTGAGGACACTCTTGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCAGAGCGTTGGGTGTTCATTAAGTGTATCTCTACCTGTACATTGGAATAACAGTCGGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCGGTGGGTGAGCTTTATATAGATGAATTCAGGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTAAATCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGTTTGAGTTCTTATTTGAAACTAGAGGGCCAGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGACCAAGATGTGCAAGAGTTGAACAAAACAACCCCTCAATCAAGTAGTGGGATGTCTCCTTATGAGGATATATCGATTAGTTTTCACATTCCAGTTGCAGGGGAAACCAGTGGCAGTGGTAGCAGAACTTGCACGCTTGAAGAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1207

Amino Acids

137.21

Weight (kDa)

6.43

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 189 1.9e-54 TIR domain
TIR_2 PF13676 14 - 111 9.4e-14 TIR domain
NB-ARC PF00931 214 - 369 1.7e-17 NB-ARC domain
NACHT PF05729 219 - 315 6.4e-07 NACHT domain
WHD_ROQ1 PF23282 436 - 507 5.4e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 696 - 781 2.1e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 707 - 777 7.2e-09 Leucine-rich repeat region
C-JID PF20160 1014 - 1163 2.4e-20 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3622
AarI CACCTGC 1 cut(s) 1928
Acc36I ACCTGC 2 cut(s) 749, 1928
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1615, 3184
AccI GTMKAC 2 cut(s) 554, 2833
AciI CCGC 7 cut(s) 70, 340, 632, 673, 880, 2551, 2596
AclI AACGTT 1 cut(s) 2161
AclWI GGATC 7 cut(s) 320, 333, 655, 668, 930, 1374, 2494
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 9 cut(s) 117, 717, 1672, 1793, 1870, 2678, 2890, 3206, 3311
AcuI CTGAAG 5 cut(s) 37, 1601, 2727, 3269, 3461
AdeI CACNNNGTG 1 cut(s) 1028
AfaI GTAC 5 cut(s) 1043, 2860, 3114, 3165, 3376
AfeI AGCGCT 1 cut(s) 1216
AfiI CCNNNNNNNGG 5 cut(s) 1412, 1615, 2609, 3184, 3572
AflII CTTAAG 1 cut(s) 1777
AflIII ACRYGT 1 cut(s) 2443
AjnI CCWGG 4 cut(s) 1531, 3043, 3318, 3433
AjuI GAANNNNNNNTTGG 2 cut(s) 2765, 2797
Alw21I GWGCWC 2 cut(s) 366, 3177
Alw26I GTCTC 9 cut(s) 1524, 1560, 1744, 1916, 1949, 2180, 2618, 2791, 3534
AlwI GGATC 7 cut(s) 320, 333, 655, 668, 930, 1374, 2494
AlwNI CAGNNNCTG 1 cut(s) 863
Ama87I CYCGRG 3 cut(s) 699, 2057, 2138
Aor51HI AGCGCT 1 cut(s) 1216
AoxI GGCC 7 cut(s) 3, 167, 462, 781, 1723, 2230, 3431
ApeKI GCWGC 3 cut(s) 182, 634, 863
ApoI RAATTY 9 cut(s) 117, 717, 1672, 1793, 1870, 2678, 2890, 3206, 3311
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AseI ATTAAT 1 cut(s) 2102
Asp700I GAANNNNTTC 6 cut(s) 592, 804, 1074, 1506, 1600, 3027
AspLEI GCGC 1 cut(s) 1217
AspS9I GGNCC 3 cut(s) 373, 1711, 3431
AsuHPI GGTGA 6 cut(s) 2412, 2669, 2713, 3200, 3326, 3457
AvaI CYCGRG 3 cut(s) 699, 2057, 2138
AvaII GGWCC 2 cut(s) 373, 1711
BaeGI GKGCMC 1 cut(s) 397
BaeI ACNNNNGTAYC 2 cut(s) 2981, 3014
BalI TGGCCA 1 cut(s) 464
BamHI GGATCC 2 cut(s) 325, 660
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 3357
BbsI GAAGAC 3 cut(s) 304, 1682, 2267
Bbv12I GWGCWC 2 cut(s) 366, 3177
BbvCI CCTCAGC 1 cut(s) 2984
BbvI GCAGC 3 cut(s) 169, 646, 850
BceAI ACGGC 1 cut(s) 2237
BcgI CGANNNNNNTGC 2 cut(s) 2038, 2072
BciT130I CCWGG 4 cut(s) 1533, 3045, 3320, 3435
BciVI GTATCC 1 cut(s) 1782
BcoDI GTCTC 9 cut(s) 1524, 1560, 1744, 1916, 1949, 2180, 2618, 2791, 3534
BfaI CTAG 7 cut(s) 198, 785, 981, 1385, 1740, 2837, 3426
BfmI CTRYAG 1 cut(s) 2257
BfoI RGCGCY 1 cut(s) 1218
BfrI CTTAAG 1 cut(s) 1777
BfuAI ACCTGC 2 cut(s) 749, 1928
BfuI GTATCC 1 cut(s) 1782
BglII AGATCT 3 cut(s) 1436, 1781, 1963
BisI GCNGC 4 cut(s) 183, 341, 635, 864
BlsI GCNGC 4 cut(s) 184, 342, 636, 865
Bme1390I CCNGG 4 cut(s) 1533, 3045, 3320, 3435
Bme18I GGWCC 2 cut(s) 373, 1711
BmeT110I CYCGRG 3 cut(s) 699, 2057, 2138
BmgT120I GGNCC 3 cut(s) 373, 1711, 3431
BmiI GGNNCC 5 cut(s) 327, 344, 374, 375, 662
BmrFI CCNGG 4 cut(s) 1533, 3045, 3320, 3435
BmrI ACTGGG 1 cut(s) 1776
BmsI GCATC 8 cut(s) 436, 1024, 1141, 1461, 2318, 2872, 2894, 2948
BmuI ACTGGG 1 cut(s) 1776
BpiI GAAGAC 3 cut(s) 304, 1682, 2267
Bpu10I CCTNAGC 2 cut(s) 1161, 2984
BpuEI CTTGAG 4 cut(s) 167, 1768, 1973, 2789
Bsa29I ATCGAT 1 cut(s) 3548
BsaAI YACGTR 1 cut(s) 1041
BsaBI GATNNNNATC 2 cut(s) 921, 1365
BsaI GGTCTC 3 cut(s) 1560, 1916, 2618
BsaJI CCNNGG 4 cut(s) 664, 1173, 1609, 2005
BsaWI WCCGGW 1 cut(s) 3181
Bsc4I CCNNNNNNNGG 5 cut(s) 1412, 1615, 2609, 3184, 3572
Bse1I ACTGG 7 cut(s) 84, 968, 1308, 1771, 2233, 3566, 3582
Bse3DI GCAATG 1 cut(s) 2956
Bse8I GATNNNNATC 2 cut(s) 921, 1365
BseBI CCWGG 4 cut(s) 1533, 3045, 3320, 3435
BseCI ATCGAT 1 cut(s) 3548
BseDI CCNNGG 4 cut(s) 664, 1173, 1609, 2005
BseJI GATNNNNATC 2 cut(s) 921, 1365
BseLI CCNNNNNNNGG 5 cut(s) 1412, 1615, 2609, 3184, 3572
BseMI GCAATG 1 cut(s) 2956
BseMII CTCAG 4 cut(s) 234, 2630, 2975, 3051
BseNI ACTGG 7 cut(s) 84, 968, 1308, 1771, 2233, 3566, 3582
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 3 cut(s) 169, 646, 850
BseYI CCCAGC 2 cut(s) 477, 1497
Bsh1285I CGRYCG 1 cut(s) 884
BshFI GGCC 7 cut(s) 5, 169, 464, 783, 1725, 2232, 3433
BshNI GGYRCC 1 cut(s) 342
BshVI ATCGAT 1 cut(s) 3548
BsiEI CGRYCG 1 cut(s) 884
BsiHKAI GWGCWC 2 cut(s) 366, 3177
BsiHKCI CYCGRG 3 cut(s) 699, 2057, 2138
BsiSI CCGG 1 cut(s) 3182
BslFI GGGAC 3 cut(s) 386, 991, 1095
BslI CCNNNNNNNGG 5 cut(s) 1412, 1615, 2609, 3184, 3572
BsmAI GTCTC 9 cut(s) 1524, 1560, 1744, 1916, 1949, 2180, 2618, 2791, 3534
BsmFI GGGAC 3 cut(s) 386, 991, 1095
BsmI GAATGC 2 cut(s) 2581, 2736
BsnI GGCC 7 cut(s) 5, 169, 464, 783, 1725, 2232, 3433
Bso31I GGTCTC 3 cut(s) 1560, 1916, 2618
BsoBI CYCGRG 3 cut(s) 699, 2057, 2138
Bsp1286I GDGCHC 3 cut(s) 366, 397, 3177
Bsp1407I TGTACA 1 cut(s) 3112
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 7 cut(s) 70, 340, 632, 673, 880, 2551, 2596
BspANI GGCC 7 cut(s) 5, 169, 464, 783, 1725, 2232, 3433
BspCNI CTCAG 4 cut(s) 233, 2631, 2976, 3052
BspDI ATCGAT 1 cut(s) 3548
BspHI TCATGA 1 cut(s) 1558
BspLI GGNNCC 5 cut(s) 327, 344, 374, 375, 662
BspMAI CTGCAG 1 cut(s) 2261
BspMI ACCTGC 2 cut(s) 749, 1928
BspPI GGATC 7 cut(s) 320, 333, 655, 668, 930, 1374, 2494
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 1777
BspTNI GGTCTC 3 cut(s) 1560, 1916, 2618
BsrDI GCAATG 1 cut(s) 2956
BsrGI TGTACA 1 cut(s) 3112
BsrI ACTGG 7 cut(s) 84, 968, 1308, 1771, 2233, 3566, 3582
BssECI CCNNGG 4 cut(s) 664, 1173, 1609, 2005
BssNAI GTATAC 1 cut(s) 555
BssSI CACGAG 1 cut(s) 3357
BssT1I CCWWGG 4 cut(s) 664, 1173, 1609, 2005
Bst1107I GTATAC 1 cut(s) 555
Bst2BI CACGAG 1 cut(s) 3357
Bst2UI CCWGG 4 cut(s) 1533, 3045, 3320, 3435
Bst4CI ACNGT 6 cut(s) 1028, 1064, 1518, 2375, 3128, 3395
Bst6I CTCTTC 2 cut(s) 19, 3256
BstAFI CTTAAG 1 cut(s) 1777
BstAPI GCANNNNNTGC 2 cut(s) 1480, 3603
BstAUI TGTACA 1 cut(s) 3112
BstBAI YACGTR 1 cut(s) 1041
BstC8I GCNNGC 7 cut(s) 671, 1342, 1397, 2555, 2600, 3291, 3608
BstDEI CTNAG 7 cut(s) 220, 1161, 2639, 2714, 2763, 2984, 3060
BstDSI CCRYGG 1 cut(s) 664
BstEII GGTNACC 1 cut(s) 2701
BstH2I RGCGCY 1 cut(s) 1218
BstHHI GCGC 1 cut(s) 1217
BstMAI GTCTC 9 cut(s) 1524, 1560, 1744, 1916, 1949, 2180, 2618, 2791, 3534
BstMCI CGRYCG 1 cut(s) 884
BstMWI GCNNNNNNNGC 8 cut(s) 675, 1471, 1480, 2764, 3078, 3152, 3594, 3603
BstNI CCWGG 4 cut(s) 1533, 3045, 3320, 3435
BstNSI RCATGY 2 cut(s) 673, 2447
BstPI GGTNACC 1 cut(s) 2701
BstSCI CCNGG 4 cut(s) 1531, 3043, 3318, 3433
BstSFI CTRYAG 1 cut(s) 2257
BstSLI GKGCMC 1 cut(s) 397
BstSNI TACGTA 1 cut(s) 1041
BstV1I GCAGC 3 cut(s) 169, 646, 850
BstV2I GAAGAC 3 cut(s) 304, 1682, 2267
BstX2I RGATCY 6 cut(s) 325, 660, 1436, 1781, 1963, 2486
BstYI RGATCY 6 cut(s) 325, 660, 1436, 1781, 1963, 2486
BstZ17I GTATAC 1 cut(s) 555
Bsu15I ATCGAT 1 cut(s) 3548
BsuI GTATCC 1 cut(s) 1782
BsuRI GGCC 7 cut(s) 5, 169, 464, 783, 1725, 2232, 3433
BsuTUI ATCGAT 1 cut(s) 3548
BtgI CCRYGG 1 cut(s) 664
BtsI GCAGTG 1 cut(s) 3595
BtsIMutI CAGTG 3 cut(s) 2864, 3589, 3595
BveI ACCTGC 2 cut(s) 749, 1928
Cac8I GCNNGC 7 cut(s) 671, 1342, 1397, 2555, 2600, 3291, 3608
CaiI CAGNNNCTG 1 cut(s) 863
CciI TCATGA 1 cut(s) 1558
CfoI GCGC 1 cut(s) 1217
Cfr13I GGNCC 3 cut(s) 373, 1711, 3431
ClaI ATCGAT 1 cut(s) 3548
Csp6I GTAC 5 cut(s) 1042, 2859, 3113, 3164, 3375
CviQI GTAC 5 cut(s) 1042, 2859, 3113, 3164, 3375
DdeI CTNAG 7 cut(s) 220, 1161, 2639, 2714, 2763, 2984, 3060
DraI TTTAAA 1 cut(s) 2493
DraIII CACNNNGTG 1 cut(s) 1028
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 2 cut(s) 19, 3256
EarI CTCTTC 2 cut(s) 19, 3256
Ecl136II GAGCTC 1 cut(s) 364
Eco105I TACGTA 1 cut(s) 1041
Eco130I CCWWGG 4 cut(s) 664, 1173, 1609, 2005
Eco147I AGGCCT 1 cut(s) 1725
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 3 cut(s) 1560, 1916, 2618
Eco47I GGWCC 2 cut(s) 373, 1711
Eco47III AGCGCT 1 cut(s) 1216
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 5 cut(s) 37, 1601, 2727, 3269, 3461
Eco88I CYCGRG 3 cut(s) 699, 2057, 2138
Eco91I GGTNACC 1 cut(s) 2701
EcoICRI GAGCTC 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 373
EcoO65I GGTNACC 1 cut(s) 2701
EcoRI GAATTC 3 cut(s) 117, 2678, 3206
EcoRII CCWGG 4 cut(s) 1531, 3043, 3318, 3433
EcoT14I CCWWGG 4 cut(s) 664, 1173, 1609, 2005
EcoT22I ATGCAT 3 cut(s) 281, 1476, 2887
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 4 cut(s) 664, 1173, 1609, 2005
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 3 cut(s) 386, 991, 1095
FauI CCCGC 2 cut(s) 77, 666
FblI GTMKAC 2 cut(s) 554, 2833
Fnu4HI GCNGC 4 cut(s) 183, 341, 635, 864
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 4 cut(s) 183, 341, 635, 864
FspBI CTAG 7 cut(s) 198, 785, 981, 1385, 1740, 2837, 3426
GlaI GCGC 1 cut(s) 1216
GluI GCNGC 4 cut(s) 183, 341, 635, 864
GsaI CCCAGC 2 cut(s) 481, 1501
HaeII RGCGCY 1 cut(s) 1218
HaeIII GGCC 7 cut(s) 5, 169, 464, 783, 1725, 2232, 3433
HapII CCGG 1 cut(s) 3182
HhaI GCGC 1 cut(s) 1217
Hin6I GCGC 1 cut(s) 1215
HinP1I GCGC 1 cut(s) 1215
HincII GTYRAC 4 cut(s) 296, 571, 930, 1629
HindII GTYRAC 4 cut(s) 296, 571, 930, 1629
HindIII AAGCTT 2 cut(s) 2021, 2765
HpaII CCGG 1 cut(s) 3182
HphI GGTGA 6 cut(s) 2412, 2669, 2713, 3200, 3326, 3457
Hpy166II GTNNAC 8 cut(s) 296, 555, 571, 930, 1514, 1629, 1711, 2834
Hpy8I GTNNAC 8 cut(s) 296, 555, 571, 930, 1514, 1629, 1711, 2834
Hpy99I CGWCG 2 cut(s) 24, 27
HpyCH4III ACNGT 6 cut(s) 1028, 1064, 1518, 2375, 3128, 3395
HpyCH4IV ACGT 3 cut(s) 1040, 1540, 2161
HpyF10VI GCNNNNNNNGC 8 cut(s) 675, 1471, 1480, 2764, 3078, 3152, 3594, 3603
HpyF3I CTNAG 7 cut(s) 220, 1161, 2639, 2714, 2763, 2984, 3060
HpySE526I ACGT 3 cut(s) 1040, 1540, 2161
HspAI GCGC 1 cut(s) 1215
KflI GGGWCCC 1 cut(s) 373
LmnI GCTCC 2 cut(s) 860, 2663
Lsp1109I GCAGC 3 cut(s) 169, 646, 850
LweI GCATC 8 cut(s) 436, 1024, 1141, 1461, 2318, 2872, 2894, 2948
MaeI CTAG 7 cut(s) 198, 785, 981, 1385, 1740, 2837, 3426
MaeII ACGT 3 cut(s) 1040, 1540, 2161
MaeIII GTNAC 5 cut(s) 956, 1372, 2418, 2684, 2701
MfeI CAATTG 3 cut(s) 186, 2214, 2929
MflI RGATCY 6 cut(s) 325, 660, 1436, 1781, 1963, 2486
MhlI GDGCHC 3 cut(s) 366, 397, 3177
MlsI TGGCCA 1 cut(s) 464
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 4 cut(s) 188, 1746, 2071, 3468
MmeI TCCRAC 2 cut(s) 1479, 2128
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 3 cut(s) 281, 1476, 2887
MroXI GAANNNNTTC 6 cut(s) 592, 804, 1074, 1506, 1600, 3027
MscI TGGCCA 1 cut(s) 464
MslI CAYNNNNRTG 2 cut(s) 587, 1619
Msp20I TGGCCA 1 cut(s) 464
MspCI CTTAAG 1 cut(s) 1777
MspI CCGG 1 cut(s) 3182
MspR9I CCNGG 4 cut(s) 1533, 3045, 3320, 3435
MunI CAATTG 3 cut(s) 186, 2214, 2929
Mva1269I GAATGC 2 cut(s) 2581, 2736
MvaI CCWGG 4 cut(s) 1533, 3045, 3320, 3435
MwoI GCNNNNNNNGC 8 cut(s) 675, 1471, 1480, 2764, 3078, 3152, 3594, 3603
NcoI CCATGG 1 cut(s) 664
NlaIV GGNNCC 5 cut(s) 327, 344, 374, 375, 662
NmeAIII GCCGAG 1 cut(s) 2319
NmuCI GTSAC 4 cut(s) 956, 1372, 2418, 2701
NsiI ATGCAT 3 cut(s) 281, 1476, 2887
NspI RCATGY 2 cut(s) 673, 2447
PaeI GCATGC 1 cut(s) 673
PaeR7I CTCGAG 2 cut(s) 699, 2057
PagI TCATGA 1 cut(s) 1558
PaqCI CACCTGC 1 cut(s) 1928
PceI AGGCCT 1 cut(s) 1725
PciI ACATGT 1 cut(s) 2443
PctI GAATGC 2 cut(s) 2581, 2736
PdmI GAANNNNTTC 6 cut(s) 592, 804, 1074, 1506, 1600, 3027
PflMI CCANNNNNTGG 2 cut(s) 1615, 3184
PfoI TCCNGGA 1 cut(s) 3043
PkrI GCNGC 4 cut(s) 184, 342, 636, 865
PleI GAGTC 4 cut(s) 188, 1746, 2070, 3467
PpsI GAGTC 4 cut(s) 188, 1746, 2070, 3467
Ppu21I YACGTR 1 cut(s) 1041
PpuMI RGGWCCY 1 cut(s) 373
PscI ACATGT 1 cut(s) 2443
PshBI ATTAAT 1 cut(s) 2102
PsiI TTATAA 1 cut(s) 3622
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 2161
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 4 cut(s) 1531, 3043, 3318, 3433
PspEI GGTNACC 1 cut(s) 2701
PspFI CCCAGC 2 cut(s) 477, 1497
PspGI CCWGG 4 cut(s) 1531, 3043, 3318, 3433
PspN4I GGNNCC 5 cut(s) 327, 344, 374, 375, 662
PspPI GGNCC 3 cut(s) 373, 1711, 3431
PspPPI RGGWCCY 1 cut(s) 373
PspXI VCTCGAGB 1 cut(s) 699
PstI CTGCAG 1 cut(s) 2261
PstNI CAGNNNCTG 1 cut(s) 863
PsuI RGATCY 6 cut(s) 325, 660, 1436, 1781, 1963, 2486
RsaI GTAC 5 cut(s) 1043, 2860, 3114, 3165, 3376
RsaNI GTAC 5 cut(s) 1042, 2859, 3113, 3164, 3375
RseI CAYNNNNRTG 2 cut(s) 587, 1619
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 4 cut(s) 183, 341, 635, 864
Sau96I GGNCC 3 cut(s) 373, 1711, 3431
SchI GAGTC 4 cut(s) 188, 1746, 2071, 3468
ScrFI CCNGG 4 cut(s) 1533, 3045, 3320, 3435
SduI GDGCHC 3 cut(s) 366, 397, 3177
SfaNI GCATC 8 cut(s) 436, 1024, 1141, 1461, 2318, 2872, 2894, 2948
SfcI CTRYAG 1 cut(s) 2257
Sfr274I CTCGAG 2 cut(s) 699, 2057
SinI GGWCC 2 cut(s) 373, 1711
SlaI CTCGAG 2 cut(s) 699, 2057
SmiMI CAYNNNNRTG 2 cut(s) 587, 1619
SmlI CTYRAG 7 cut(s) 146, 699, 1747, 1777, 1952, 2057, 2768
SmoI CTYRAG 7 cut(s) 146, 699, 1747, 1777, 1952, 2057, 2768
SnaBI TACGTA 1 cut(s) 1041
SphI GCATGC 1 cut(s) 673
SseBI AGGCCT 1 cut(s) 1725
SsiI CCGC 7 cut(s) 70, 340, 632, 673, 880, 2551, 2596
SspI AATATT 3 cut(s) 77, 1326, 3055
SspMI CTAG 7 cut(s) 198, 785, 981, 1385, 1740, 2837, 3426
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 1725
StyD4I CCNGG 4 cut(s) 1531, 3043, 3318, 3433
StyI CCWWGG 4 cut(s) 664, 1173, 1609, 2005
TaaI ACNGT 6 cut(s) 1028, 1064, 1518, 2375, 3128, 3395
TaiI ACGT 3 cut(s) 1043, 1543, 2164
TaqI TCGA 5 cut(s) 25, 700, 2058, 2752, 3548
TaqII GACCGA 1 cut(s) 750
TatI WGTACW 3 cut(s) 2858, 3112, 3163
TauI GCSGC 1 cut(s) 343
TscAI CASTG 3 cut(s) 2871, 3589, 3595
TseFI GTSAC 4 cut(s) 956, 1372, 2418, 2701
TseI GCWGC 3 cut(s) 182, 634, 863
Tsp45I GTSAC 4 cut(s) 956, 1372, 2418, 2701
TspGWI ACGGA 2 cut(s) 538, 3452
TspRI CASTG 3 cut(s) 2871, 3589, 3595
Van91I CCANNNNNTGG 2 cut(s) 1615, 3184
Vha464I CTTAAG 1 cut(s) 1777
VpaK11BI GGWCC 2 cut(s) 373, 1711
VspI ATTAAT 1 cut(s) 2102
XapI RAATTY 9 cut(s) 117, 717, 1672, 1793, 1870, 2678, 2890, 3206, 3311
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 2 cut(s) 673, 2447
XcmI CCANNNNNNNNNTGG 3 cut(s) 820, 2003, 3080
XhoI CTCGAG 2 cut(s) 699, 2057
XmiI GTMKAC 2 cut(s) 554, 2833
XmnI GAANNNNTTC 6 cut(s) 592, 804, 1074, 1506, 1600, 3027
XspI CTAG 7 cut(s) 198, 785, 981, 1385, 1740, 2837, 3426
Zsp2I ATGCAT 3 cut(s) 281, 1476, 2887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.