RLG00000002602

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000105
Physical Location & Seq
Forward (+)
36842885 .. 36846505
3621 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002602

Sequence Viewer

Length: 2775 bp
ATGGCGTCAACCTCCTCTCGTCGTCAATGGAAATACGATGTCTTTCTCAATTTCAGGGGGTTTGATACGCGCGGGCGTTTAGCCGGTGATCTGTATTCTGCACTAGTTGGGAAAGGAATTCTCACCTTTATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGACTCTAGATGTGCGATTGTCATTCTCTCACGGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATGGGCCAACAAGTCTTCCACGGCGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGGTTTAATCTCATCTGGGAATCCAAATTAGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATGAGGACAGACTAAATGCGTGGAGAGCTGCTTTGATTGATGTCGCCAAGCTTTCTGGCTGGGATGGCAAGAACTATCCAAGTGTAACAAAACTAGTTGAGGAAATTGTTAATCACATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTCATGATTTATCAAGGAATTACATACGTACACAGCTTGGTGAGGTGCGTTTTATAGGGATCCATGGCATGCGGGGGATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAGCAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATGAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAAAATTATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATATTGATGCTACATACAAGGTTAAAGGGTTAAAATGTCATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTTCCCCACCAGAAGATTATTTACATCTGTGCAACCTTATTTTAGGGTATGCTCAGGGGCTTCCATTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCACATAAACGAATCATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCGAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAATTATCTCCGACGACAAAGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGAAAACGTAGTAGATTATGGTCTCATGAAGACGTCAACAATGTGCTGAAGAAAAATAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAAAATTGAGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCTGAGTTTGTTGGAAGTATGGAACGTTTGTCGGTGCTTTTTCTTGATGAGACTGCCATTGAGGAACTGCCGGTGTCAATTGAACGGCTGAGTAGCCTTGTGTCATTAAATCTGAACAACTGCAGAAATCTTGTCTGTCTTCCGAGTACCATCAATAAATTGAAGTCTATTAAAGATCTTAATCTTTCTGGATGCTTGAGACTCGGCAAACATCCGGTAAATGTGGGGGTGATGGACTGTTTTGAGGAAACTGATGTGAATGGTGGGTCTGCAATACAAATGTCATCTACCCATGATCACAAAAAATATGTGAGAGGTCCAATCTTTCATGGATGCAAAGTAGTTTGGCGATCTTTAAAAAAGTTCTTGCCATCTGGATCGGTGCAAAAAATGAATACAGAGCCAAAGAGTTTCCACTTGCCTATATCACAAAAAGTGAATACAGAGCCCATGAGTTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGTAGGAGCATTTGTCAACGAATTTGGTTACATGCCCTCTTTGGTGTCCTTGAATCTAAGTGGGAACAACTTTATTCGTCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGTGTTGTCAGACCTTCCATCAAATAGTAGTCTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTCGAAATGCTGAAGACATTCATGTATCAGGTACTCCCTAAGTGTGTGCGTGCGCGTTTCTGCTCATATACTTATAGACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

925

Amino Acids

104.64

Weight (kDa)

8.58

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 186 1.9e-41 TIR domain
TIR_2 PF13676 14 - 98 4.1e-11 TIR domain
NB-ARC PF00931 211 - 366 2.6e-19 NB-ARC domain
NACHT PF05729 216 - 314 3.6e-07 NACHT domain
WHD_ROQ1 PF23282 433 - 505 4.9e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 542 - 635 2.3e-06 Leucine-rich repeat region
LRR_13 PF23286 602 - 686 1.5e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 613 - 681 3.1e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1646
AatII GACGTC 1 cut(s) 1563
Acc36I ACCTGC 2 cut(s) 740, 1646
AccB1I GGYRCC 1 cut(s) 333
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 545
AccII CGCG 3 cut(s) 70, 72, 2746
AciI CCGC 6 cut(s) 72, 331, 589, 664, 871, 2314
AclI AACGTT 1 cut(s) 1879
AclWI GGATC 7 cut(s) 311, 324, 646, 659, 921, 1365, 2239
AcsI RAATTY 5 cut(s) 117, 708, 1588, 2396, 2608
AcuI CTGAAG 3 cut(s) 1595, 2445, 2723
AcyI GRCGYC 2 cut(s) 5, 1560
AdeI CACNNNGTG 1 cut(s) 313
AfaI GTAC 4 cut(s) 622, 2002, 2578, 2724
AfiI CCNNNNNNNGG 3 cut(s) 313, 1403, 2327
AhlI ACTAGT 2 cut(s) 103, 502
AjnI CCWGG 2 cut(s) 964, 1525
AjuI GAANNNNNNNTTGG 2 cut(s) 2483, 2515
Alw26I GTCTC 8 cut(s) 1518, 1554, 1634, 1667, 1778, 1898, 2047, 2509
AlwI GGATC 7 cut(s) 311, 324, 646, 659, 921, 1365, 2239
AlwNI CAGNNNCTG 1 cut(s) 854
Ama87I CYCGRG 2 cut(s) 690, 1775
AoxI GGCC 3 cut(s) 167, 292, 772
ApeKI GCWGC 3 cut(s) 182, 437, 854
ApoI RAATTY 5 cut(s) 117, 708, 1588, 2396, 2608
AseI ATTAAT 1 cut(s) 1820
Asp700I GAANNNNTTC 5 cut(s) 583, 795, 1065, 1500, 2708
AspLEI GCGC 2 cut(s) 72, 2746
AspS9I GGNCC 3 cut(s) 292, 962, 2171
AsuHPI GGTGA 4 cut(s) 98, 115, 644, 2095
AsuII TTCGAA 1 cut(s) 2694
AvaI CYCGRG 2 cut(s) 690, 1775
AvaII GGWCC 2 cut(s) 962, 2171
BamHI GGATCC 2 cut(s) 316, 651
BanI GGYRCC 1 cut(s) 333
BanII GRGCYC 1 cut(s) 2304
BarI GAAGNNNNNNTAC 2 cut(s) 1104, 1136
BauI CACGAG 1 cut(s) 1172
BbsI GAAGAC 5 cut(s) 295, 1563, 1985, 2447, 2711
BbvI GCAGC 3 cut(s) 169, 424, 841
BceAI ACGGC 2 cut(s) 325, 1955
BcgI CGANNNNNNTGC 2 cut(s) 1756, 1790
BciT130I CCWGG 2 cut(s) 966, 1527
BclI TGATCA 1 cut(s) 2149
BcoDI GTCTC 8 cut(s) 1518, 1554, 1634, 1667, 1778, 1898, 2047, 2509
BcuI ACTAGT 2 cut(s) 103, 502
BfaI CTAG 6 cut(s) 104, 198, 503, 776, 1376, 2555
BfmI CTRYAG 1 cut(s) 1975
BfuAI ACCTGC 2 cut(s) 740, 1646
BglII AGATCT 3 cut(s) 1427, 1681, 2029
BisI GCNGC 4 cut(s) 183, 332, 438, 855
BlsI GCNGC 4 cut(s) 184, 333, 439, 856
Bme1390I CCNGG 2 cut(s) 966, 1527
Bme18I GGWCC 2 cut(s) 962, 2171
BmeT110I CYCGRG 2 cut(s) 690, 1775
BmgT120I GGNCC 3 cut(s) 292, 962, 2171
BmiI GGNNCC 3 cut(s) 318, 335, 653
BmrFI CCNGG 2 cut(s) 966, 1527
BmsI GCATC 7 cut(s) 1015, 1455, 2036, 2177, 2590, 2612, 2666
BpiI GAAGAC 5 cut(s) 295, 1563, 1985, 2447, 2711
Bpu10I CCTNAGC 1 cut(s) 1152
Bpu14I TTCGAA 1 cut(s) 2694
BpuEI CTTGAG 4 cut(s) 167, 1691, 2071, 2507
BsaAI YACGTR 1 cut(s) 620
BsaBI GATNNNNATC 3 cut(s) 912, 1356, 2034
BsaHI GRCGYC 2 cut(s) 5, 1560
BsaI GGTCTC 2 cut(s) 1554, 1634
BsaJI CCNNGG 4 cut(s) 307, 655, 965, 1723
BsaWI WCCGGW 1 cut(s) 2068
BsaXI ACNNNNNCTCC 2 cut(s) 2373, 2403
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 1403, 2327
Bse118I RCCGGY 2 cut(s) 83, 1924
Bse1I ACTGG 2 cut(s) 959, 1299
Bse3DI GCAATG 1 cut(s) 775
Bse8I GATNNNNATC 3 cut(s) 912, 1356, 2034
BseBI CCWGG 2 cut(s) 966, 1527
BseDI CCNNGG 4 cut(s) 307, 655, 965, 1723
BseJI GATNNNNATC 3 cut(s) 912, 1356, 2034
BseLI CCNNNNNNNGG 3 cut(s) 313, 1403, 2327
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 4 cut(s) 1166, 1848, 1934, 2348
BseNI ACTGG 2 cut(s) 959, 1299
BseRI GAGGAG 2 cut(s) 4, 1652
BseXI GCAGC 3 cut(s) 169, 424, 841
BseYI CCCAGC 2 cut(s) 468, 1491
BsgI GTGCAG 1 cut(s) 84
Bsh1236I CGCG 3 cut(s) 70, 72, 2746
BshFI GGCC 3 cut(s) 169, 294, 774
BshNI GGYRCC 1 cut(s) 333
BsiHKCI CYCGRG 2 cut(s) 690, 1775
BsiSI CCGG 3 cut(s) 84, 1925, 2069
BslI CCNNNNNNNGG 3 cut(s) 313, 1403, 2327
BsmAI GTCTC 8 cut(s) 1518, 1554, 1634, 1667, 1778, 1898, 2047, 2509
BsmI GAATGC 2 cut(s) 591, 2690
BsnI GGCC 3 cut(s) 169, 294, 774
Bso31I GGTCTC 2 cut(s) 1554, 1634
BsoBI CYCGRG 2 cut(s) 690, 1775
Bsp119I TTCGAA 1 cut(s) 2694
Bsp1286I GDGCHC 1 cut(s) 2304
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 6 cut(s) 72, 331, 589, 664, 871, 2314
BspANI GGCC 3 cut(s) 169, 294, 774
BspCNI CTCAG 4 cut(s) 1165, 1849, 1935, 2349
BspFNI CGCG 3 cut(s) 70, 72, 2746
BspHI TCATGA 3 cut(s) 595, 1057, 1552
BspLI GGNNCC 3 cut(s) 318, 335, 653
BspMAI CTGCAG 1 cut(s) 1979
BspMI ACCTGC 2 cut(s) 740, 1646
BspPI GGATC 7 cut(s) 311, 324, 646, 659, 921, 1365, 2239
BspT104I TTCGAA 1 cut(s) 2694
BspT107I GGYRCC 1 cut(s) 333
BspTNI GGTCTC 2 cut(s) 1554, 1634
BsrDI GCAATG 1 cut(s) 775
BsrFI RCCGGY 2 cut(s) 83, 1924
BsrI ACTGG 2 cut(s) 959, 1299
BssAI RCCGGY 2 cut(s) 83, 1924
BssECI CCNNGG 4 cut(s) 307, 655, 965, 1723
BssNAI GTATAC 1 cut(s) 546
BssNI GRCGYC 2 cut(s) 5, 1560
BssSI CACGAG 1 cut(s) 1172
BssT1I CCWWGG 2 cut(s) 655, 1723
Bst1107I GTATAC 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 1172
Bst2UI CCWGG 2 cut(s) 966, 1527
Bst4CI ACNGT 3 cut(s) 1461, 1512, 2093
BstACI GRCGYC 2 cut(s) 5, 1560
BstAPI GCANNNNNTGC 1 cut(s) 1474
BstBAI YACGTR 1 cut(s) 620
BstBI TTCGAA 1 cut(s) 2694
BstC8I GCNNGC 6 cut(s) 74, 662, 1333, 1388, 2318, 2742
BstDEI CTNAG 7 cut(s) 1152, 1857, 1943, 2357, 2432, 2481, 2730
BstDSI CCRYGG 2 cut(s) 307, 655
BstFNI CGCG 3 cut(s) 70, 72, 2746
BstHHI GCGC 2 cut(s) 72, 2746
BstMAI GTCTC 8 cut(s) 1518, 1554, 1634, 1667, 1778, 1898, 2047, 2509
BstMWI GCNNNNNNNGC 4 cut(s) 434, 474, 1474, 2482
BstNI CCWGG 2 cut(s) 966, 1527
BstNSI RCATGY 2 cut(s) 664, 2410
BstSCI CCNGG 2 cut(s) 964, 1525
BstSFI CTRYAG 1 cut(s) 1975
BstSNI TACGTA 1 cut(s) 620
BstUI CGCG 3 cut(s) 70, 72, 2746
BstV1I GCAGC 3 cut(s) 169, 424, 841
BstV2I GAAGAC 5 cut(s) 295, 1563, 1985, 2447, 2711
BstX2I RGATCY 5 cut(s) 316, 651, 1427, 1681, 2029
BstYI RGATCY 5 cut(s) 316, 651, 1427, 1681, 2029
BstZ17I GTATAC 1 cut(s) 546
BsuRI GGCC 3 cut(s) 169, 294, 774
BtgI CCRYGG 2 cut(s) 307, 655
BtsIMutI CAGTG 2 cut(s) 1206, 2582
BveI ACCTGC 2 cut(s) 740, 1646
Cac8I GCNNGC 6 cut(s) 74, 662, 1333, 1388, 2318, 2742
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 3 cut(s) 595, 1057, 1552
CfoI GCGC 2 cut(s) 72, 2746
Cfr10I RCCGGY 2 cut(s) 83, 1924
Cfr13I GGNCC 3 cut(s) 292, 962, 2171
CseI GACGC 1 cut(s) 146
Csp6I GTAC 4 cut(s) 621, 2001, 2577, 2723
CviQI GTAC 4 cut(s) 621, 2001, 2577, 2723
DdeI CTNAG 7 cut(s) 1152, 1857, 1943, 2357, 2432, 2481, 2730
DraI TTTAAA 1 cut(s) 2211
DraIII CACNNNGTG 1 cut(s) 313
Eco105I TACGTA 1 cut(s) 620
Eco130I CCWWGG 2 cut(s) 655, 1723
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 2304
Eco31I GGTCTC 2 cut(s) 1554, 1634
Eco47I GGWCC 2 cut(s) 962, 2171
Eco57I CTGAAG 3 cut(s) 1595, 2445, 2723
Eco88I CYCGRG 2 cut(s) 690, 1775
EcoRI GAATTC 1 cut(s) 117
EcoRII CCWGG 2 cut(s) 964, 1525
EcoT14I CCWWGG 2 cut(s) 655, 1723
EcoT22I ATGCAT 3 cut(s) 281, 1470, 2605
EcoT38I GRGCYC 1 cut(s) 2304
ErhI CCWWGG 2 cut(s) 655, 1723
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FauI CCCGC 3 cut(s) 65, 596, 657
FbaI TGATCA 1 cut(s) 2149
FblI GTMKAC 1 cut(s) 545
Fnu4HI GCNGC 4 cut(s) 183, 332, 438, 855
FriOI GRGCYC 1 cut(s) 2304
Fsp4HI GCNGC 4 cut(s) 183, 332, 438, 855
FspBI CTAG 6 cut(s) 104, 198, 503, 776, 1376, 2555
GlaI GCGC 2 cut(s) 71, 2745
GluI GCNGC 4 cut(s) 183, 332, 438, 855
GsaI CCCAGC 2 cut(s) 472, 1495
HaeIII GGCC 3 cut(s) 169, 294, 774
HapII CCGG 3 cut(s) 84, 1925, 2069
HgaI GACGC 1 cut(s) 146
HhaI GCGC 2 cut(s) 72, 2746
Hin1I GRCGYC 2 cut(s) 5, 1560
Hin6I GCGC 2 cut(s) 70, 2744
HinP1I GCGC 2 cut(s) 70, 2744
HincII GTYRAC 5 cut(s) 9, 562, 921, 1564, 2392
HindII GTYRAC 5 cut(s) 9, 562, 921, 1564, 2392
HindIII AAGCTT 3 cut(s) 458, 1739, 2483
HpaII CCGG 3 cut(s) 84, 1925, 2069
HphI GGTGA 4 cut(s) 98, 115, 644, 2095
Hpy166II GTNNAC 8 cut(s) 9, 546, 562, 623, 921, 1508, 1564, 2392
Hpy188I TCNGA 8 cut(s) 325, 1278, 1447, 1665, 1968, 1998, 2352, 2533
Hpy8I GTNNAC 8 cut(s) 9, 546, 562, 623, 921, 1508, 1564, 2392
Hpy99I CGWCG 2 cut(s) 24, 1451
HpyAV CCTTC 8 cut(s) 330, 339, 880, 1078, 1342, 1682, 2469, 2546
HpyCH4III ACNGT 3 cut(s) 1461, 1512, 2093
HpyCH4IV ACGT 4 cut(s) 619, 1534, 1560, 1879
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 474, 1474, 2482
HpyF3I CTNAG 7 cut(s) 1152, 1857, 1943, 2357, 2432, 2481, 2730
HpySE526I ACGT 4 cut(s) 619, 1534, 1560, 1879
Hsp92I GRCGYC 2 cut(s) 5, 1560
HspAI GCGC 2 cut(s) 70, 2744
Ksp22I TGATCA 1 cut(s) 2149
LmnI GCTCC 2 cut(s) 851, 2381
Lsp1109I GCAGC 3 cut(s) 169, 424, 841
LweI GCATC 7 cut(s) 1015, 1455, 2036, 2177, 2590, 2612, 2666
MaeI CTAG 6 cut(s) 104, 198, 503, 776, 1376, 2555
MaeII ACGT 4 cut(s) 619, 1534, 1560, 1879
MaeIII GTNAC 3 cut(s) 493, 1363, 2402
MfeI CAATTG 3 cut(s) 186, 1932, 2647
MflI RGATCY 5 cut(s) 316, 651, 1427, 1681, 2029
MhlI GDGCHC 1 cut(s) 2304
MlyI GAGTC 3 cut(s) 188, 1789, 2049
MmeI TCCRAC 2 cut(s) 1470, 1846
Mph1103I ATGCAT 3 cut(s) 281, 1470, 2605
MroXI GAANNNNTTC 5 cut(s) 583, 795, 1065, 1500, 2708
MslI CAYNNNNRTG 3 cut(s) 128, 578, 1268
MspI CCGG 3 cut(s) 84, 1925, 2069
MspR9I CCNGG 2 cut(s) 966, 1527
MunI CAATTG 3 cut(s) 186, 1932, 2647
Mva1269I GAATGC 2 cut(s) 591, 2690
MvaI CCWGG 2 cut(s) 966, 1527
MvnI CGCG 3 cut(s) 70, 72, 2746
MwoI GCNNNNNNNGC 4 cut(s) 434, 474, 1474, 2482
NcoI CCATGG 1 cut(s) 655
NlaIV GGNNCC 3 cut(s) 318, 335, 653
NmeAIII GCCGAG 1 cut(s) 2037
NmuCI GTSAC 1 cut(s) 1363
NsiI ATGCAT 3 cut(s) 281, 1470, 2605
NspI RCATGY 2 cut(s) 664, 2410
NspV TTCGAA 1 cut(s) 2694
PaeI GCATGC 1 cut(s) 664
PaeR7I CTCGAG 2 cut(s) 690, 1775
PagI TCATGA 3 cut(s) 595, 1057, 1552
PaqCI CACCTGC 1 cut(s) 1646
PceI AGGCCT 1 cut(s) 169
PctI GAATGC 2 cut(s) 591, 2690
PdmI GAANNNNTTC 5 cut(s) 583, 795, 1065, 1500, 2708
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 4 cut(s) 184, 333, 439, 856
PleI GAGTC 3 cut(s) 188, 1788, 2049
PpsI GAGTC 3 cut(s) 188, 1788, 2049
Ppu21I YACGTR 1 cut(s) 620
PshBI ATTAAT 1 cut(s) 1820
Psp1406I AACGTT 1 cut(s) 1879
Psp6I CCWGG 2 cut(s) 964, 1525
PspFI CCCAGC 2 cut(s) 468, 1491
PspGI CCWGG 2 cut(s) 964, 1525
PspN4I GGNNCC 3 cut(s) 318, 335, 653
PspPI GGNCC 3 cut(s) 292, 962, 2171
PspXI VCTCGAGB 1 cut(s) 690
PstI CTGCAG 1 cut(s) 1979
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 5 cut(s) 316, 651, 1427, 1681, 2029
RsaI GTAC 4 cut(s) 622, 2002, 2578, 2724
RsaNI GTAC 4 cut(s) 621, 2001, 2577, 2723
RseI CAYNNNNRTG 3 cut(s) 128, 578, 1268
SatI GCNGC 4 cut(s) 183, 332, 438, 855
Sau96I GGNCC 3 cut(s) 292, 962, 2171
SchI GAGTC 3 cut(s) 188, 1789, 2049
ScrFI CCNGG 2 cut(s) 966, 1527
SduI GDGCHC 1 cut(s) 2304
SfaNI GCATC 7 cut(s) 1015, 1455, 2036, 2177, 2590, 2612, 2666
SfcI CTRYAG 1 cut(s) 1975
Sfr274I CTCGAG 2 cut(s) 690, 1775
SfuI TTCGAA 1 cut(s) 2694
SinI GGWCC 2 cut(s) 962, 2171
SlaI CTCGAG 2 cut(s) 690, 1775
SmiMI CAYNNNNRTG 3 cut(s) 128, 578, 1268
SmlI CTYRAG 6 cut(s) 146, 690, 1670, 1775, 2050, 2486
SmoI CTYRAG 6 cut(s) 146, 690, 1670, 1775, 2050, 2486
SnaBI TACGTA 1 cut(s) 620
SpeI ACTAGT 2 cut(s) 103, 502
SphI GCATGC 1 cut(s) 664
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 6 cut(s) 72, 331, 589, 664, 871, 2314
SspI AATATT 1 cut(s) 1317
SspMI CTAG 6 cut(s) 104, 198, 503, 776, 1376, 2555
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 2 cut(s) 964, 1525
StyI CCWWGG 2 cut(s) 655, 1723
TaaI ACNGT 3 cut(s) 1461, 1512, 2093
TaiI ACGT 4 cut(s) 622, 1537, 1563, 1882
TaqI TCGA 4 cut(s) 691, 1463, 1776, 2694
TaqII GACCGA 1 cut(s) 741
TatI WGTACW 1 cut(s) 2576
TauI GCSGC 1 cut(s) 334
TscAI CASTG 2 cut(s) 1213, 2589
TseFI GTSAC 1 cut(s) 1363
TseI GCWGC 3 cut(s) 182, 437, 854
Tsp45I GTSAC 1 cut(s) 1363
TspGWI ACGGA 2 cut(s) 238, 529
TspRI CASTG 2 cut(s) 1213, 2589
Van91I CCANNNNNTGG 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 962, 2171
VspI ATTAAT 1 cut(s) 1820
XapI RAATTY 5 cut(s) 117, 708, 1588, 2396, 2608
XbaI TCTAGA 2 cut(s) 197, 2554
XceI RCATGY 2 cut(s) 664, 2410
XcmI CCANNNNNNNNNTGG 2 cut(s) 811, 1721
XhoI CTCGAG 2 cut(s) 690, 1775
XmiI GTMKAC 1 cut(s) 545
XmnI GAANNNNTTC 5 cut(s) 583, 795, 1065, 1500, 2708
XspI CTAG 6 cut(s) 104, 198, 503, 776, 1376, 2555
ZraI GACGTC 1 cut(s) 1561
Zsp2I ATGCAT 3 cut(s) 281, 1470, 2605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.