Rmu_sc0001209.1_g000022

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001209.1
OG0000105
Physical Location & Seq
Reverse (-)
77782 .. 82095
4314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001209.1_g000022.1.cds

Sequence Viewer

Length: 3222 bp
atgactacccaagcagatattccttcttcttctgctcctctccctccgatccctaaatacggtgtctttctcagttccagcggagaagacaccgatcaaaattttacagatcatctctgttatgctctgaaccggaaggggatattgacatttagagatggtgaagaactcgagaggggaaaagcaattgaagaatcaagatgtgcaattgtggttctttcaggaaactacaccaatccatggtgtttggatgcgattgcaaagatcgttgaatgcaaggaagtgatgatgaaactgggagtactcccagtctttcttgatgtgaatccatctgatgtacgaaaacaaacaggggatcatttcggggaagccttcttgaagcatgaagaggcctttaaacacgaaccggagaaggtgcgcaggtggagacgtgctcttacttcagtaaccaatctctctggatggcttgtgcaaaatcgtcatgaagccaaagttattcaagaaatgacagaatttattctcagagaattctttcctagaatcccaagtgtacctgagggattagttgggatggattctcacgtgaacgaaatgctctcatacttggaagtggggcgtcctggagttcgtaccgtagggatttgggggatggggggtatcggaaagacaactattgcaaaagctatttatgaaaggatttattctcagtttgaaggtcacgtctttcttgagaatgtcagagaatcaagtcgtatgccaggtggtagtattggtttacaagaggaacttctttcaaacttgctgagacgtaatgtaaatgtacgtgacatcactaggggaagaaccgtgatcagggaaagactacatactagaatggttcttatcgttgttgatgatgtggatgaactagaacaattggaagtattatgtggtgctgctagttggttcgggtcagggagtagaatcatcataacatcaagagatgatcatttgctgaaagcatttagtgtggagaacatatacaaggtcaatgcattatctgatgttgaggctcttcagctgttttgttcaagagccttcaagaaggaccaggttggagaagatgttctcaagctttctaaggagtttgtaaaatatgctgctggccttccattagctcttcaagtattggctgcatctctccgtggtaaggaaaaaactctgtggtcaagtgcattggatagactaaaacagaaacctgatagagaaattatgaatgtgcttaaggtaagttatgatggactagatgacttagaaaaggttatttttttagacattgcatgcttctttaaaggagagaacgtagattgtgtagaaagaatactaggggtcgattattttccaaactttggtatcagagttcttctggatagatctctagtaactgtggttggaagaaaattatggatgcatgacctgctgcaacaattgggtaaagcaattgttattcaagaatgtcttgaagagcctggcaatcgtagcaggttgtgggttccgcaagaaatcatccatgtactcgagaagaataagggttcaagtgcactgcgagcgatagccttagactttccaaaaacagaagagatacactgccactcagaggcattctctgtgatgcccagacttgagtttctccaaatccgtaatgtgcatatgaaggaaggccccagttatcttcctgggggattagtagttctggaatggagtgggaccccagattttacacatgctccagggcttgaaagattaattcttgaaggttgtacaagtttagtggacatccacccatccattgctcacctggagaggcttattagcttgaatcttaaagattgcagaagtcttgcaagtcttccaagtaagcttgaaacaaaatgccttcgacttcttgttctttcaggctgttcaaaagtcaagaaaattccagattttgtggaatctatggaaggcttgttagaactttatttggatgggactgctatagaggaaatacctatatcagttcgacttctgactggcctcattttattaaatttgagagattgcaaaaatctcaagagacttccaggagacattggcaatttaaagtccctcaagcgtctcaatatttgtggatgctcaaaacttgaaacactaccagacagcttgggaaagatagactgcttggaagagcttgatgcggctgggggtagttccataagcaatataccatcctctatttcccgtttggaaagtcttgaagtgttatctctttgtggatgtaaagggatgtcaggcaacatgacaagttatccactcagttttctgttgcacaaaagtgattcaggtggcttgcgttctttaactgagctaaatctcagtgactgtaatcttcaggagggaagcataccggaagattttggctgcttattccctttagtttcactaaatctaagcaaaaacaactttgttagtcttccaaaaagcatcagacagctttcaaagcttcgaaacttgaatttggaaagttgtaagactcttcaaaaattgccagacctttcatcaagcataaagttttatggggcagagggcggtatttcacaggaaaggctgtcatcatgtttcaatttgattaattgctccaaattggttgtcaatcaaccatgcaacaatatagcattgaaaatgctatcgagatttcttcagggaattccttctgcaggaaataaatttgaaactataattcctggcagtgaaatctctgagctgttcagtcatccaagtgtgggaccagtaataagtatggagctacctcgacaatggcacaagaataagtggatgggatatgcattttgtgcttgttttgttatccgtcgagatcttccagttccagctaaccgacttggtaaatggaagtttgggacccatagcactgcaaatggtcttcgatgcgaagtgaagcctgagaatctgggggtggctggctggtgtccttcctttgcctgcagccaagaattaggccaaattgagtctgaacacctttggctatcttttgtctctggtgattattttggaacagcatggcaagaaaactgtcgtaacattgagtttacattcaaaacattaggcactggtatggaggtgaagaagtgtggagtccgtttgatatacgagcaagatttagatttgattggaatgaaaagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1073

Amino Acids

119.79

Weight (kDa)

6.65

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 411
Acc16I TGCGCA 1 cut(s) 419
Acc36I ACCTGC 3 cut(s) 411, 1464, 1512
AccB7I CCANNNNNTGG 2 cut(s) 240, 1388
AciI CCGC 4 cut(s) 81, 1535, 2216, 2607
AclWI GGATC 2 cut(s) 43, 363
AcsI RAATTY 8 cut(s) 100, 512, 527, 1956, 2068, 2533, 2724, 2744
AcuI CTGAAG 4 cut(s) 426, 1040, 2393, 2702
AcvI CACGTG 1 cut(s) 583
AcyI GRCGYC 1 cut(s) 616
AfaI GTAC 7 cut(s) 303, 339, 552, 631, 822, 1554, 1801
AfiI CCNNNNNNNGG 8 cut(s) 59, 240, 852, 1189, 1388, 1636, 2735, 2801
AflII CTTAAG 1 cut(s) 1262
AjiI CACGTC 2 cut(s) 431, 721
AjnI CCWGG 9 cut(s) 619, 757, 1089, 1507, 1714, 1768, 1836, 2101, 2761
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AleI CACNNNNGTG 1 cut(s) 2352
AloI GAACNNNNNNTCC 4 cut(s) 1089, 1121, 1713, 1745
Alw21I GWGCWC 2 cut(s) 436, 1582
Alw26I GTCTC 6 cut(s) 421, 799, 2089, 2100, 2141, 3076
Alw44I GTGCAC 1 cut(s) 1578
AlwI GGATC 2 cut(s) 43, 363
AlwNI CAGNNNCTG 1 cut(s) 2400
Ama87I CYCGRG 2 cut(s) 170, 1556
AoxI GGCC 5 cut(s) 390, 1144, 1699, 2053, 3034
ApaLI GTGCAC 1 cut(s) 1578
ApeKI GCWGC 6 cut(s) 935, 1139, 1172, 1459, 2439, 3021
ApoI RAATTY 8 cut(s) 100, 512, 527, 1956, 2068, 2533, 2724, 2744
ArsI GACNNNNNNTTYG 2 cut(s) 2652, 2684
AseI ATTAAT 2 cut(s) 1784, 2649
Asp700I GAANNNNTTC 4 cut(s) 516, 531, 1104, 2728
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 5 cut(s) 1087, 1700, 1746, 2804, 2937
AsuHPI GGTGA 4 cut(s) 173, 1826, 3089, 3169
AsuII TTCGAA 1 cut(s) 2524
AvaI CYCGRG 2 cut(s) 170, 1556
AvaII GGWCC 4 cut(s) 1087, 1746, 2804, 2937
AxyI CCTNAGG 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 1582
BaeI ACNNNNGTAYC 2 cut(s) 1375, 1408
BbrPI CACGTG 1 cut(s) 583
BbsI GAAGAC 4 cut(s) 93, 1880, 2483, 2951
Bbv12I GWGCWC 2 cut(s) 436, 1582
BbvI GCAGC 6 cut(s) 922, 1126, 1159, 1446, 2426, 3033
BciT130I CCWGG 9 cut(s) 621, 759, 1091, 1509, 1716, 1770, 1838, 2103, 2763
BclI TGATCA 2 cut(s) 849, 985
BcoDI GTCTC 6 cut(s) 421, 799, 2089, 2100, 2141, 3076
BfaI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
BfmI CTRYAG 3 cut(s) 2016, 2733, 3019
BfrI CTTAAG 1 cut(s) 1262
BfuAI ACCTGC 3 cut(s) 411, 1464, 1512
BglII AGATCT 2 cut(s) 1412, 2893
BisI GCNGC 7 cut(s) 936, 1140, 1173, 1460, 2217, 2440, 3022
BlsI GCNGC 7 cut(s) 937, 1141, 1174, 1461, 2218, 2441, 3023
BmcAI AGTACT 1 cut(s) 303
Bme1390I CCNGG 9 cut(s) 621, 759, 1091, 1509, 1716, 1770, 1838, 2103, 2763
Bme18I GGWCC 4 cut(s) 1087, 1746, 2804, 2937
BmeT110I CYCGRG 2 cut(s) 170, 1556
BmgBI CACGTC 2 cut(s) 431, 721
BmgT120I GGNCC 5 cut(s) 1087, 1700, 1746, 2804, 2937
BmiI GGNNCC 7 cut(s) 1533, 1702, 1747, 1748, 2805, 2938, 2939
BmrFI CCNGG 9 cut(s) 621, 759, 1091, 1509, 1716, 1770, 1838, 2103, 2763
BmrI ACTGGG 3 cut(s) 302, 305, 1698
BmsI GCATC 8 cut(s) 241, 1184, 1437, 1641, 2141, 2203, 2511, 2954
BmuI ACTGGG 3 cut(s) 302, 305, 1698
BpiI GAAGAC 4 cut(s) 93, 1880, 2483, 2951
BplI GAGNNNNNCTC 4 cut(s) 418, 450, 1628, 1660
BpmI CTGGAG 3 cut(s) 642, 1752, 1859
Bpu14I TTCGAA 1 cut(s) 2524
BpuEI CTTGAG 5 cut(s) 749, 1094, 1682, 2075, 2114
BsaAI YACGTR 2 cut(s) 583, 824
BsaBI GATNNNNATC 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 616
BsaJI CCNNGG 4 cut(s) 239, 1183, 1715, 1769
BsaWI WCCGGW 3 cut(s) 132, 406, 2425
BsaXI ACNNNNNCTCC 6 cut(s) 1089, 1119, 1326, 1356, 1750, 1780
Bsc4I CCNNNNNNNGG 8 cut(s) 59, 240, 852, 1189, 1388, 1636, 2735, 2801
Bse1I ACTGG 7 cut(s) 300, 308, 1704, 2056, 2807, 2900, 3151
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 2 cut(s) 1314, 1827
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 9 cut(s) 621, 759, 1091, 1509, 1716, 1770, 1838, 2103, 2763
BseDI CCNNGG 4 cut(s) 239, 1183, 1715, 1769
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 8 cut(s) 59, 240, 852, 1189, 1388, 1636, 2735, 2801
BseMI GCAATG 2 cut(s) 1314, 1827
BseNI ACTGG 7 cut(s) 300, 308, 1704, 2056, 2807, 2900, 3151
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 1582
BseXI GCAGC 6 cut(s) 922, 1126, 1159, 1446, 2426, 3033
BseYI CCCAGC 1 cut(s) 2219
BshFI GGCC 5 cut(s) 392, 1146, 1701, 2055, 3036
BsiHKAI GWGCWC 2 cut(s) 436, 1582
BsiHKCI CYCGRG 2 cut(s) 170, 1556
BsiSI CCGG 3 cut(s) 133, 407, 2426
BslFI GGGAC 5 cut(s) 1759, 2023, 2110, 2817, 2950
BslI CCNNNNNNNGG 8 cut(s) 59, 240, 852, 1189, 1388, 1636, 2735, 2801
BsmAI GTCTC 6 cut(s) 421, 799, 2089, 2100, 2141, 3076
BsmBI CGTCTC 3 cut(s) 421, 799, 2141
BsmFI GGGAC 5 cut(s) 1759, 2023, 2110, 2817, 2950
BsmI GAATGC 2 cut(s) 278, 1640
BsnI GGCC 5 cut(s) 392, 1146, 1701, 2055, 3036
BsoBI CYCGRG 2 cut(s) 170, 1556
Bsp119I TTCGAA 1 cut(s) 2524
Bsp1286I GDGCHC 2 cut(s) 436, 1582
Bsp1407I TGTACA 1 cut(s) 1799
Bsp143I GATC 9 cut(s) 48, 94, 109, 264, 355, 849, 985, 1412, 2893
Bsp19I CCATGG 1 cut(s) 239
BspACI CCGC 4 cut(s) 81, 1535, 2216, 2607
BspANI GGCC 5 cut(s) 392, 1146, 1701, 2055, 3036
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 7 cut(s) 1533, 1702, 1747, 1748, 2805, 2938, 2939
BspMAI CTGCAG 2 cut(s) 2737, 3023
BspMI ACCTGC 3 cut(s) 411, 1464, 1512
BspPI GGATC 2 cut(s) 43, 363
BspQI GCTCTTC 4 cut(s) 1059, 1164, 1497, 2199
BspT104I TTCGAA 1 cut(s) 2524
BspTI CTTAAG 1 cut(s) 1262
BsrDI GCAATG 2 cut(s) 1314, 1827
BsrGI TGTACA 1 cut(s) 1799
BsrI ACTGG 7 cut(s) 300, 308, 1704, 2056, 2807, 2900, 3151
BssECI CCNNGG 4 cut(s) 239, 1183, 1715, 1769
BssMI GATC 9 cut(s) 48, 94, 109, 264, 355, 849, 985, 1412, 2893
BssNI GRCGYC 1 cut(s) 616
BssT1I CCWWGG 1 cut(s) 239
Bst2UI CCWGG 9 cut(s) 621, 759, 1091, 1509, 1716, 1770, 1838, 2103, 2763
Bst4CI ACNGT 6 cut(s) 62, 634, 847, 1426, 2402, 3110
Bst6I CTCTTC 7 cut(s) 381, 1059, 1164, 1497, 1611, 2199, 2559
BstACI GRCGYC 1 cut(s) 616
BstAFI CTTAAG 1 cut(s) 1262
BstAPI GCANNNNNTGC 2 cut(s) 1456, 2870
BstAUI TGTACA 1 cut(s) 1799
BstBAI YACGTR 2 cut(s) 583, 824
BstBI TTCGAA 1 cut(s) 2524
BstC8I GCNNGC 6 cut(s) 1144, 1321, 1587, 2369, 2998, 3019
BstDSI CCRYGG 2 cut(s) 239, 1183
BstENI CCTNNNNNAGG 1 cut(s) 2733
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 9 cut(s) 51, 97, 112, 267, 358, 852, 988, 1415, 2896
BstMAI GTCTC 6 cut(s) 421, 799, 2089, 2100, 2141, 3076
BstMBI GATC 9 cut(s) 48, 94, 109, 264, 355, 849, 985, 1412, 2893
BstMWI GCNNNNNNNGC 5 cut(s) 1456, 1518, 1586, 2518, 2870
BstNI CCWGG 9 cut(s) 621, 759, 1091, 1509, 1716, 1770, 1838, 2103, 2763
BstNSI RCATGY 2 cut(s) 1323, 1766
BstSCI CCNGG 9 cut(s) 619, 757, 1089, 1507, 1714, 1768, 1836, 2101, 2761
BstSFI CTRYAG 3 cut(s) 2016, 2733, 3019
BstSLI GKGCMC 1 cut(s) 1582
BstV1I GCAGC 6 cut(s) 922, 1126, 1159, 1446, 2426, 3033
BstV2I GAAGAC 4 cut(s) 93, 1880, 2483, 2951
BstX2I RGATCY 2 cut(s) 1412, 2893
BstYI RGATCY 2 cut(s) 1412, 2893
Bsu36I CCTNAGG 1 cut(s) 555
BsuRI GGCC 5 cut(s) 392, 1146, 1701, 2055, 3036
BtgI CCRYGG 2 cut(s) 239, 1183
BtrI CACGTC 2 cut(s) 431, 721
BtsI GCAGTG 4 cut(s) 1580, 1624, 2773, 2946
BtsIMutI CAGTG 6 cut(s) 1580, 1624, 2401, 2773, 2946, 3144
BveI ACCTGC 3 cut(s) 411, 1464, 1512
Cac8I GCNNGC 6 cut(s) 1144, 1321, 1587, 2369, 2998, 3019
CaiI CAGNNNCTG 1 cut(s) 2400
CciI TCATGA 1 cut(s) 481
CfoI GCGC 1 cut(s) 420
Cfr13I GGNCC 5 cut(s) 1087, 1700, 1746, 2804, 2937
CseI GACGC 2 cut(s) 605, 2123
CsiI ACCWGGT 1 cut(s) 1089
Csp6I GTAC 7 cut(s) 302, 338, 551, 630, 821, 1553, 1800
CspCI CAANNNNNGTGG 4 cut(s) 1791, 1826, 2128, 2163
CviQI GTAC 7 cut(s) 302, 338, 551, 630, 821, 1553, 1800
DpnI GATC 9 cut(s) 50, 96, 111, 266, 357, 851, 987, 1414, 2895
DpnII GATC 9 cut(s) 48, 94, 109, 264, 355, 849, 985, 1412, 2893
DraI TTTAAA 3 cut(s) 397, 1330, 2121
Eam1104I CTCTTC 7 cut(s) 381, 1059, 1164, 1497, 1611, 2199, 2559
EarI CTCTTC 7 cut(s) 381, 1059, 1164, 1497, 1611, 2199, 2559
Eco130I CCWWGG 1 cut(s) 239
Eco147I AGGCCT 1 cut(s) 392
Eco47I GGWCC 4 cut(s) 1087, 1746, 2804, 2937
Eco57I CTGAAG 4 cut(s) 426, 1040, 2393, 2702
Eco72I CACGTG 1 cut(s) 583
Eco81I CCTNAGG 1 cut(s) 555
Eco88I CYCGRG 2 cut(s) 170, 1556
EcoNI CCTNNNNNAGG 1 cut(s) 2733
EcoO109I RGGNCCY 3 cut(s) 1700, 1746, 2937
EcoRI GAATTC 2 cut(s) 527, 2724
EcoRII CCWGG 9 cut(s) 619, 757, 1089, 1507, 1714, 1768, 1836, 2101, 2761
EcoT14I CCWWGG 1 cut(s) 239
EcoT22I ATGCAT 3 cut(s) 1036, 1452, 2866
ErhI CCWWGG 1 cut(s) 239
Esp3I CGTCTC 3 cut(s) 421, 799, 2141
FalI AAGNNNNNCTT 4 cut(s) 771, 803, 1482, 1514
FaqI GGGAC 5 cut(s) 1759, 2023, 2110, 2817, 2950
FauNDI CATATG 1 cut(s) 1689
FbaI TGATCA 2 cut(s) 849, 985
Fnu4HI GCNGC 7 cut(s) 936, 1140, 1173, 1460, 2217, 2440, 3022
Fsp4HI GCNGC 7 cut(s) 936, 1140, 1173, 1460, 2217, 2440, 3022
FspBI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
FspI TGCGCA 1 cut(s) 419
GlaI GCGC 1 cut(s) 419
GluI GCNGC 7 cut(s) 936, 1140, 1173, 1460, 2217, 2440, 3022
GsaI CCCAGC 1 cut(s) 2223
GsuI CTGGAG 3 cut(s) 642, 1752, 1859
HaeIII GGCC 5 cut(s) 392, 1146, 1701, 2055, 3036
HapII CCGG 3 cut(s) 133, 407, 2426
HgaI GACGC 2 cut(s) 605, 2123
HhaI GCGC 1 cut(s) 420
Hin1I GRCGYC 1 cut(s) 616
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HindIII AAGCTT 3 cut(s) 1112, 1898, 2519
HpaII CCGG 3 cut(s) 133, 407, 2426
HphI GGTGA 4 cut(s) 173, 1826, 3089, 3169
Hpy166II GTNNAC 6 cut(s) 551, 586, 776, 1580, 1813, 3126
Hpy8I GTNNAC 6 cut(s) 551, 586, 776, 1580, 1813, 3126
Hpy99I CGWCG 1 cut(s) 2892
HpyCH4III ACNGT 6 cut(s) 62, 634, 847, 1426, 2402, 3110
HpyCH4IV ACGT 6 cut(s) 430, 582, 720, 808, 823, 1341
HpyF10VI GCNNNNNNNGC 5 cut(s) 1456, 1518, 1586, 2518, 2870
HpySE526I ACGT 6 cut(s) 430, 582, 720, 808, 823, 1341
Hsp92I GRCGYC 1 cut(s) 616
HspAI GCGC 1 cut(s) 418
KflI GGGWCCC 2 cut(s) 1746, 2937
Ksp22I TGATCA 2 cut(s) 849, 985
Kzo9I GATC 9 cut(s) 48, 94, 109, 264, 355, 849, 985, 1412, 2893
LguI GCTCTTC 4 cut(s) 1059, 1164, 1497, 2199
LmnI GCTCC 4 cut(s) 40, 1771, 2660, 2821
Lsp1109I GCAGC 6 cut(s) 922, 1126, 1159, 1446, 2426, 3033
LweI GCATC 8 cut(s) 241, 1184, 1437, 1641, 2141, 2203, 2511, 2954
MabI ACCWGGT 1 cut(s) 1089
MaeI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
MaeII ACGT 6 cut(s) 430, 582, 720, 808, 823, 1341
MaeIII GTNAC 6 cut(s) 445, 716, 824, 1420, 2396, 3113
MalI GATC 9 cut(s) 50, 96, 111, 266, 357, 851, 987, 1414, 2895
MboI GATC 9 cut(s) 48, 94, 109, 264, 355, 849, 985, 1412, 2893
MfeI CAATTG 5 cut(s) 186, 207, 914, 1466, 1479
MflI RGATCY 2 cut(s) 1412, 2893
MhlI GDGCHC 2 cut(s) 436, 1582
MlyI GAGTC 3 cut(s) 2545, 3053, 3180
MmeI TCCRAC 2 cut(s) 1075, 1411
Mph1103I ATGCAT 3 cut(s) 1036, 1452, 2866
MroXI GAANNNNTTC 4 cut(s) 516, 531, 1104, 2728
MseI TTAA 9 cut(s) 396, 1263, 1329, 1784, 1863, 2066, 2120, 2378, 2649
MslI CAYNNNNRTG 3 cut(s) 2352, 2796, 3149
MspA1I CMGCKG 2 cut(s) 81, 1060
MspCI CTTAAG 1 cut(s) 1262
MspI CCGG 3 cut(s) 133, 407, 2426
MspR9I CCNGG 9 cut(s) 621, 759, 1091, 1509, 1716, 1770, 1838, 2103, 2763
MunI CAATTG 5 cut(s) 186, 207, 914, 1466, 1479
Mva1269I GAATGC 2 cut(s) 278, 1640
MvaI CCWGG 9 cut(s) 621, 759, 1091, 1509, 1716, 1770, 1838, 2103, 2763
MwoI GCNNNNNNNGC 5 cut(s) 1456, 1518, 1586, 2518, 2870
NcoI CCATGG 1 cut(s) 239
NdeI CATATG 1 cut(s) 1689
NdeII GATC 9 cut(s) 48, 94, 109, 264, 355, 849, 985, 1412, 2893
NlaIV GGNNCC 7 cut(s) 1533, 1702, 1747, 1748, 2805, 2938, 2939
NmuCI GTSAC 3 cut(s) 716, 824, 2396
NsbI TGCGCA 1 cut(s) 419
NsiI ATGCAT 3 cut(s) 1036, 1452, 2866
NspI RCATGY 2 cut(s) 1323, 1766
NspV TTCGAA 1 cut(s) 2524
OliI CACNNNNGTG 1 cut(s) 2352
PaeI GCATGC 1 cut(s) 1323
PaeR7I CTCGAG 2 cut(s) 170, 1556
PagI TCATGA 1 cut(s) 481
PaqCI CACCTGC 1 cut(s) 411
PceI AGGCCT 1 cut(s) 392
PciSI GCTCTTC 4 cut(s) 1059, 1164, 1497, 2199
PctI GAATGC 2 cut(s) 278, 1640
PdmI GAANNNNTTC 4 cut(s) 516, 531, 1104, 2728
PflMI CCANNNNNTGG 2 cut(s) 240, 1388
PfoI TCCNGGA 2 cut(s) 619, 2101
PkrI GCNGC 7 cut(s) 937, 1141, 1174, 1461, 2218, 2441, 3023
PleI GAGTC 3 cut(s) 2545, 3052, 3179
PmaCI CACGTG 1 cut(s) 583
PmlI CACGTG 1 cut(s) 583
PpsI GAGTC 3 cut(s) 2545, 3052, 3179
Ppu21I YACGTR 2 cut(s) 583, 824
PpuMI RGGWCCY 2 cut(s) 1746, 2937
PshBI ATTAAT 2 cut(s) 1784, 2649
Psp5II RGGWCCY 2 cut(s) 1746, 2937
Psp6I CCWGG 9 cut(s) 619, 757, 1089, 1507, 1714, 1768, 1836, 2101, 2761
PspCI CACGTG 1 cut(s) 583
PspFI CCCAGC 1 cut(s) 2219
PspGI CCWGG 9 cut(s) 619, 757, 1089, 1507, 1714, 1768, 1836, 2101, 2761
PspN4I GGNNCC 7 cut(s) 1533, 1702, 1747, 1748, 2805, 2938, 2939
PspPI GGNCC 5 cut(s) 1087, 1700, 1746, 2804, 2937
PspPPI RGGWCCY 2 cut(s) 1746, 2937
PstI CTGCAG 2 cut(s) 2737, 3023
PstNI CAGNNNCTG 1 cut(s) 2400
PsuI RGATCY 2 cut(s) 1412, 2893
PvuII CAGCTG 1 cut(s) 1060
RsaI GTAC 7 cut(s) 303, 339, 552, 631, 822, 1554, 1801
RsaNI GTAC 7 cut(s) 302, 338, 551, 630, 821, 1553, 1800
RseI CAYNNNNRTG 3 cut(s) 2352, 2796, 3149
SapI GCTCTTC 4 cut(s) 1059, 1164, 1497, 2199
SaqAI TTAA 9 cut(s) 396, 1263, 1329, 1784, 1863, 2066, 2120, 2378, 2649
SatI GCNGC 7 cut(s) 936, 1140, 1173, 1460, 2217, 2440, 3022
Sau3AI GATC 9 cut(s) 48, 94, 109, 264, 355, 849, 985, 1412, 2893
Sau96I GGNCC 5 cut(s) 1087, 1700, 1746, 2804, 2937
ScaI AGTACT 1 cut(s) 303
SchI GAGTC 3 cut(s) 2545, 3053, 3180
ScrFI CCNGG 9 cut(s) 621, 759, 1091, 1509, 1716, 1770, 1838, 2103, 2763
SduI GDGCHC 2 cut(s) 436, 1582
SexAI ACCWGGT 1 cut(s) 1089
SfaNI GCATC 8 cut(s) 241, 1184, 1437, 1641, 2141, 2203, 2511, 2954
SfcI CTRYAG 3 cut(s) 2016, 2733, 3019
Sfr274I CTCGAG 2 cut(s) 170, 1556
SfuI TTCGAA 1 cut(s) 2524
SinI GGWCC 4 cut(s) 1087, 1746, 2804, 2937
SlaI CTCGAG 2 cut(s) 170, 1556
SmiMI CAYNNNNRTG 3 cut(s) 2352, 2796, 3149
SmlI CTYRAG 8 cut(s) 170, 728, 1109, 1262, 1556, 1661, 2090, 2129
SmoI CTYRAG 8 cut(s) 170, 728, 1109, 1262, 1556, 1661, 2090, 2129
SphI GCATGC 1 cut(s) 1323
SseBI AGGCCT 1 cut(s) 392
SsiI CCGC 4 cut(s) 81, 1535, 2216, 2607
SspI AATATT 1 cut(s) 2143
SspMI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 9 cut(s) 619, 757, 1089, 1507, 1714, 1768, 1836, 2101, 2761
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 6 cut(s) 62, 634, 847, 1426, 2402, 3110
TaiI ACGT 6 cut(s) 433, 585, 723, 811, 826, 1344
TatI WGTACW 3 cut(s) 301, 1552, 1799
TauI GCSGC 1 cut(s) 2219
Tru1I TTAA 9 cut(s) 396, 1263, 1329, 1784, 1863, 2066, 2120, 2378, 2649
Tru9I TTAA 9 cut(s) 396, 1263, 1329, 1784, 1863, 2066, 2120, 2378, 2649
TscAI CASTG 6 cut(s) 1587, 1631, 2401, 2773, 2953, 3151
TseFI GTSAC 3 cut(s) 716, 824, 2396
TseI GCWGC 6 cut(s) 935, 1139, 1172, 1459, 2439, 3021
Tsp45I GTSAC 3 cut(s) 716, 824, 2396
TspDTI ATGAA 8 cut(s) 305, 399, 498, 705, 918, 1268, 1706, 2565
TspGWI ACGGA 4 cut(s) 1172, 1667, 2876, 3164
TspRI CASTG 6 cut(s) 1587, 1631, 2401, 2773, 2953, 3151
Van91I CCANNNNNTGG 2 cut(s) 240, 1388
Vha464I CTTAAG 1 cut(s) 1262
VneI GTGCAC 1 cut(s) 1578
VpaK11BI GGWCC 4 cut(s) 1087, 1746, 2804, 2937
VspI ATTAAT 2 cut(s) 1784, 2649
XagI CCTNNNNNAGG 1 cut(s) 2733
XapI RAATTY 8 cut(s) 100, 512, 527, 1956, 2068, 2533, 2724, 2744
XceI RCATGY 2 cut(s) 1323, 1766
XcmI CCANNNNNNNNNTGG 1 cut(s) 1834
XhoI CTCGAG 2 cut(s) 170, 1556
XmnI GAANNNNTTC 4 cut(s) 516, 531, 1104, 2728
XspI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
ZrmI AGTACT 1 cut(s) 303
Zsp2I ATGCAT 3 cut(s) 1036, 1452, 2866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.