RchiOBHm_Chr1g0321301

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
8891390 .. 8897064
5675 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55137

Sequence Viewer

Length: 2019 bp
ATGGTAATGGAGTTGACAAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAAGTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACTTGCCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTGTCCCTTAAGATCTCTCCCAAAAAAATTTAAAGCACATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAAAAGCTATGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTAAAAAAATTGTGGAGAGCCCCGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTCGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACAGCTGACTGGCCTTGTGTCATTGAATGTAAGCAACTGCAGAAAACTTGTTTGTCTTCCAAGCACCGTCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAGCATCAGTTAAATATGGAGGAGATGGACTGTTTTGAGGAAACTGATGTGAAGAGTGGGTATGCAATAGAAATGTCATCTACCCTTGATCGCATAAAACATGTGAGAGGTTTAATCTTTCATCGATGCAAAGTAGTTAGGGGATCTTTAAATAAGTTTTTGCCTTCTGGATTGGTGCAAAAAGTGAATACACAGCCAATGAGTTTCTGCTTGCCTATATCTCAAAATGGGAATACAGAGCCAACGAGTTTCGGCTTGCCTATATCTCAAAAAGTGAATACAGAGCCGACGAGTTTCCGCTTGCCTATTTCTGGTCTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACAAATTCGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCACTTTCCTGCAGGCATTCGATTGCTTTCTAAACTTGAGAACTTTAACTTGGAGAATTGTAAGAGACTTAAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAATAGATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATGAAGGATGCAATAACATAGCATTTGAAATGCTGAGGACATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCATTAAGTGTATCTCTACCTGTGCATTGGAATAACAGTCAGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCAGTGGATGAGCTTTATACAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTAAATCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGCTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGAAGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTAGGATGTCTCAAGAGGATATATTGGTTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

672

Amino Acids

76.29

Weight (kDa)

8.14

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_3 PF07725 67 - 85 2.3e-06 Leucine Rich Repeat
LRR_13 PF23286 158 - 242 5.9e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 169 - 239 2.4e-09 Leucine-rich repeat region
C-JID PF20160 491 - 640 3.6e-21 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 314
Acc36I ACCTGC 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 1615
AccI GTMKAC 1 cut(s) 1264
AciI CCGC 1 cut(s) 1027
AclI AACGTT 1 cut(s) 547
AclWI GGATC 1 cut(s) 880
AcsI RAATTY 6 cut(s) 182, 256, 1109, 1321, 1637, 1742
AcuI CTGAAG 2 cut(s) 1700, 1892
AfaI GTAC 3 cut(s) 1291, 1596, 1807
AfiI CCNNNNNNNGG 2 cut(s) 1040, 1615
AflII CTTAAG 1 cut(s) 163
AflIII ACRYGT 1 cut(s) 829
AhdI GACNNNNNGTC 1 cut(s) 156
AjnI CCWGG 2 cut(s) 1474, 1749
AjuI GAANNNNNNNTTGG 2 cut(s) 1196, 1228
AluBI AGCT 6 cut(s) 238, 409, 610, 1624, 1684, 1837
AluI AGCT 6 cut(s) 238, 409, 610, 1624, 1684, 1837
Alw21I GWGCWC 1 cut(s) 1608
Alw26I GTCTC 8 cut(s) 69, 130, 335, 446, 566, 569, 1222, 1965
AlwI GGATC 1 cut(s) 880
Ama87I CYCGRG 2 cut(s) 443, 524
AoxI GGCC 3 cut(s) 109, 616, 1862
ApoI RAATTY 6 cut(s) 182, 256, 1109, 1321, 1637, 1742
AseI ATTAAT 1 cut(s) 488
Asp700I GAANNNNTTC 1 cut(s) 1458
AspS9I GGNCC 2 cut(s) 97, 1862
AsuC2I CCSGG 1 cut(s) 1866
AsuHPI GGTGA 4 cut(s) 1100, 1144, 1757, 1888
AvaI CYCGRG 2 cut(s) 443, 524
AvaII GGWCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 314
BauI CACGAG 1 cut(s) 1788
BbsI GAAGAC 2 cut(s) 68, 653
Bbv12I GWGCWC 1 cut(s) 1608
BbvCI CCTCAGC 1 cut(s) 1415
BccI CCATC 6 cut(s) 390, 748, 1261, 1277, 1512, 1617
BcgI CGANNNNNNTGC 4 cut(s) 424, 458, 557, 591
BciT130I CCWGG 2 cut(s) 1476, 1751
BcnI CCSGG 1 cut(s) 1866
BcoDI GTCTC 8 cut(s) 69, 130, 335, 446, 566, 569, 1222, 1965
BfaI CTAG 1 cut(s) 1268
BfmI CTRYAG 2 cut(s) 643, 1173
BfrI CTTAAG 1 cut(s) 163
BfuAI ACCTGC 1 cut(s) 314
BglII AGATCT 2 cut(s) 167, 349
Bme1390I CCNGG 3 cut(s) 1476, 1751, 1866
Bme18I GGWCC 1 cut(s) 97
BmeRI GACNNNNNGTC 1 cut(s) 156
BmeT110I CYCGRG 2 cut(s) 443, 524
BmgT120I GGNCC 2 cut(s) 97, 1862
BmrFI CCNGG 3 cut(s) 1476, 1751, 1866
BmrI ACTGGG 1 cut(s) 162
BmsI GCATC 6 cut(s) 704, 742, 845, 1303, 1325, 1379
BmuI ACTGGG 1 cut(s) 162
BpiI GAAGAC 2 cut(s) 68, 653
Bpu10I CCTNAGC 1 cut(s) 1415
BpuEI CTTGAG 5 cut(s) 154, 359, 1220, 1859, 1947
BpuMI CCSGG 1 cut(s) 1866
Bsa29I ATCGAT 1 cut(s) 853
BsaJI CCNNGG 1 cut(s) 391
Bsc4I CCNNNNNNNGG 2 cut(s) 1040, 1615
Bse1I ACTGG 4 cut(s) 157, 619, 1613, 1990
Bse3DI GCAATG 1 cut(s) 1387
BseBI CCWGG 2 cut(s) 1476, 1751
BseCI ATCGAT 1 cut(s) 853
BseDI CCNNGG 1 cut(s) 391
BseGI GGATG 6 cut(s) 365, 719, 1394, 1609, 1624, 1962
BseLI CCNNNNNNNGG 2 cut(s) 1040, 1615
BseMI GCAATG 1 cut(s) 1387
BseMII CTCAG 3 cut(s) 1061, 1406, 1482
BseNI ACTGG 4 cut(s) 157, 619, 1613, 1990
BseRI GAGGAG 2 cut(s) 131, 764
BshFI GGCC 3 cut(s) 111, 618, 1864
BshVI ATCGAT 1 cut(s) 853
BsiHKAI GWGCWC 1 cut(s) 1608
BsiHKCI CYCGRG 2 cut(s) 443, 524
BsiSI CCGG 1 cut(s) 1865
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 2 cut(s) 1040, 1615
BsmAI GTCTC 8 cut(s) 69, 130, 335, 446, 566, 569, 1222, 1965
BsmFI GGGAC 1 cut(s) 144
BsmI GAATGC 1 cut(s) 1179
BsnI GGCC 3 cut(s) 111, 618, 1864
BsoBI CYCGRG 2 cut(s) 443, 524
Bsp1286I GDGCHC 2 cut(s) 314, 1608
Bsp143I GATC 6 cut(s) 167, 349, 817, 872, 1340, 1762
BspACI CCGC 1 cut(s) 1027
BspANI GGCC 3 cut(s) 111, 618, 1864
BspCNI CTCAG 3 cut(s) 1062, 1407, 1483
BspDI ATCGAT 1 cut(s) 853
BspMAI CTGCAG 2 cut(s) 647, 1177
BspMI ACCTGC 1 cut(s) 314
BspPI GGATC 1 cut(s) 880
BspTI CTTAAG 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 1387
BsrI ACTGG 4 cut(s) 157, 619, 1613, 1990
BssECI CCNNGG 1 cut(s) 391
BssMI GATC 6 cut(s) 167, 349, 817, 872, 1340, 1762
BssSI CACGAG 1 cut(s) 1788
BssT1I CCWWGG 1 cut(s) 391
Bst2BI CACGAG 1 cut(s) 1788
Bst2UI CCWGG 2 cut(s) 1476, 1751
Bst4CI ACNGT 4 cut(s) 673, 761, 1559, 1826
Bst6I CTCTTC 2 cut(s) 776, 1687
BstAFI CTTAAG 1 cut(s) 163
BstAPI GCANNNNNTGC 2 cut(s) 1915, 1998
BstC8I GCNNGC 7 cut(s) 731, 941, 986, 1031, 1177, 1722, 2003
BstDEI CTNAG 5 cut(s) 71, 1070, 1145, 1415, 1491
BstEII GGTNACC 1 cut(s) 1132
BstF5I GGATG 6 cut(s) 365, 719, 1394, 1609, 1624, 1962
BstKTI GATC 6 cut(s) 170, 352, 820, 875, 1343, 1765
BstMAI GTCTC 8 cut(s) 69, 130, 335, 446, 566, 569, 1222, 1965
BstMBI GATC 6 cut(s) 167, 349, 817, 872, 1340, 1762
BstMWI GCNNNNNNNGC 5 cut(s) 117, 1583, 1915, 1989, 1998
BstNI CCWGG 2 cut(s) 1476, 1751
BstNSI RCATGY 1 cut(s) 833
BstPI GGTNACC 1 cut(s) 1132
BstSCI CCNGG 3 cut(s) 1474, 1749, 1864
BstSFI CTRYAG 2 cut(s) 643, 1173
BstV2I GAAGAC 2 cut(s) 68, 653
BstX2I RGATCY 3 cut(s) 167, 349, 872
BstYI RGATCY 3 cut(s) 167, 349, 872
Bsu15I ATCGAT 1 cut(s) 853
BsuRI GGCC 3 cut(s) 111, 618, 1864
BsuTUI ATCGAT 1 cut(s) 853
BtsCI GGATG 6 cut(s) 365, 719, 1394, 1609, 1624, 1962
BtsIMutI CAGTG 3 cut(s) 1295, 1620, 1983
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 7 cut(s) 731, 941, 986, 1031, 1177, 1722, 2003
Cfr13I GGNCC 2 cut(s) 97, 1862
ClaI ATCGAT 1 cut(s) 853
Csp6I GTAC 3 cut(s) 1290, 1595, 1806
CviAII CATG 4 cut(s) 194, 830, 1426, 1601
CviQI GTAC 3 cut(s) 1290, 1595, 1806
DdeI CTNAG 5 cut(s) 71, 1070, 1145, 1415, 1491
DpnI GATC 6 cut(s) 169, 351, 819, 874, 1342, 1764
DpnII GATC 6 cut(s) 167, 349, 817, 872, 1340, 1762
DraI TTTAAA 2 cut(s) 187, 879
DriI GACNNNNNGTC 1 cut(s) 156
Eam1104I CTCTTC 2 cut(s) 776, 1687
Eam1105I GACNNNNNGTC 1 cut(s) 156
EarI CTCTTC 2 cut(s) 776, 1687
Eco130I CCWWGG 1 cut(s) 391
Eco147I AGGCCT 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 97
Eco57I CTGAAG 2 cut(s) 1700, 1892
Eco88I CYCGRG 2 cut(s) 443, 524
Eco91I GGTNACC 1 cut(s) 1132
EcoO65I GGTNACC 1 cut(s) 1132
EcoRI GAATTC 1 cut(s) 1637
EcoRII CCWGG 2 cut(s) 1474, 1749
EcoT14I CCWWGG 1 cut(s) 391
EcoT22I ATGCAT 1 cut(s) 1318
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 4 cut(s) 197, 833, 1429, 1604
FaqI GGGAC 1 cut(s) 144
FatI CATG 4 cut(s) 193, 829, 1425, 1600
FblI GTMKAC 1 cut(s) 1264
FokI GGATG 6 cut(s) 372, 726, 1401, 1596, 1631, 1969
FriOI GRGCYC 1 cut(s) 314
FspBI CTAG 1 cut(s) 1268
HaeIII GGCC 3 cut(s) 111, 618, 1864
HapII CCGG 1 cut(s) 1865
Hin1II CATG 4 cut(s) 197, 833, 1429, 1604
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HindIII AAGCTT 1 cut(s) 407
HpaII CCGG 1 cut(s) 1865
HphI GGTGA 4 cut(s) 1100, 1144, 1757, 1888
Hpy166II GTNNAC 4 cut(s) 15, 97, 1165, 1265
Hpy188I TCNGA 7 cut(s) 333, 1065, 1246, 1509, 1762, 1872, 1899
Hpy8I GTNNAC 4 cut(s) 15, 97, 1165, 1265
Hpy99I CGWCG 1 cut(s) 1021
HpyAV CCTTC 9 cut(s) 110, 238, 350, 903, 1085, 1259, 1379, 1867, 1873
HpyCH4III ACNGT 4 cut(s) 673, 761, 1559, 1826
HpyCH4IV ACGT 2 cut(s) 547, 1058
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 1583, 1915, 1989, 1998
HpyF3I CTNAG 5 cut(s) 71, 1070, 1145, 1415, 1491
HpySE526I ACGT 2 cut(s) 547, 1058
Hsp92II CATG 4 cut(s) 197, 833, 1429, 1604
Kzo9I GATC 6 cut(s) 167, 349, 817, 872, 1340, 1762
LmnI GCTCC 1 cut(s) 1094
LweI GCATC 6 cut(s) 704, 742, 845, 1303, 1325, 1379
MaeI CTAG 1 cut(s) 1268
MaeII ACGT 2 cut(s) 547, 1058
MaeIII GTNAC 2 cut(s) 1115, 1132
MalI GATC 6 cut(s) 169, 351, 819, 874, 1342, 1764
MboI GATC 6 cut(s) 167, 349, 817, 872, 1340, 1762
MboII GAAGA 6 cut(s) 68, 529, 653, 793, 1704, 1751
MfeI CAATTG 2 cut(s) 600, 1360
MflI RGATCY 3 cut(s) 167, 349, 872
MhlI GDGCHC 2 cut(s) 314, 1608
MlyI GAGTC 3 cut(s) 132, 457, 1899
MmeI TCCRAC 1 cut(s) 514
Mph1103I ATGCAT 1 cut(s) 1318
MroXI GAANNNNTTC 1 cut(s) 1458
MslI CAYNNNNRTG 1 cut(s) 1509
MspA1I CMGCKG 1 cut(s) 610
MspCI CTTAAG 1 cut(s) 163
MspI CCGG 1 cut(s) 1865
MspR9I CCNGG 3 cut(s) 1476, 1751, 1866
MunI CAATTG 2 cut(s) 600, 1360
Mva1269I GAATGC 1 cut(s) 1179
MvaI CCWGG 2 cut(s) 1476, 1751
MwoI GCNNNNNNNGC 5 cut(s) 117, 1583, 1915, 1989, 1998
NciI CCSGG 1 cut(s) 1866
NdeII GATC 6 cut(s) 167, 349, 817, 872, 1340, 1762
NlaIII CATG 4 cut(s) 197, 833, 1429, 1604
NmeAIII GCCGAG 1 cut(s) 705
NmuCI GTSAC 1 cut(s) 1132
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 1 cut(s) 833
PaeR7I CTCGAG 1 cut(s) 443
PaqCI CACCTGC 1 cut(s) 314
PceI AGGCCT 1 cut(s) 111
PciI ACATGT 1 cut(s) 829
PctI GAATGC 1 cut(s) 1179
PdmI GAANNNNTTC 1 cut(s) 1458
PfeI GAWTC 7 cut(s) 334, 378, 421, 473, 520, 1141, 1437
PflMI CCANNNNNTGG 1 cut(s) 1615
PfoI TCCNGGA 1 cut(s) 1474
PleI GAGTC 3 cut(s) 132, 456, 1898
PpsI GAGTC 3 cut(s) 132, 456, 1898
PscI ACATGT 1 cut(s) 829
PshBI ATTAAT 1 cut(s) 488
Psp1406I AACGTT 1 cut(s) 547
Psp6I CCWGG 2 cut(s) 1474, 1749
PspEI GGTNACC 1 cut(s) 1132
PspGI CCWGG 2 cut(s) 1474, 1749
PspPI GGNCC 2 cut(s) 97, 1862
PstI CTGCAG 2 cut(s) 647, 1177
PsuI RGATCY 3 cut(s) 167, 349, 872
PvuII CAGCTG 1 cut(s) 610
RsaI GTAC 3 cut(s) 1291, 1596, 1807
RsaNI GTAC 3 cut(s) 1290, 1595, 1806
RseI CAYNNNNRTG 1 cut(s) 1509
Sau3AI GATC 6 cut(s) 167, 349, 817, 872, 1340, 1762
Sau96I GGNCC 2 cut(s) 97, 1862
SbfI CCTGCAGG 1 cut(s) 1177
SchI GAGTC 3 cut(s) 132, 457, 1899
ScrFI CCNGG 3 cut(s) 1476, 1751, 1866
SdaI CCTGCAGG 1 cut(s) 1177
SduI GDGCHC 2 cut(s) 314, 1608
SfaNI GCATC 6 cut(s) 704, 742, 845, 1303, 1325, 1379
SfcI CTRYAG 2 cut(s) 643, 1173
Sfr274I CTCGAG 1 cut(s) 443
SinI GGWCC 1 cut(s) 97
SlaI CTCGAG 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 1509
SmlI CTYRAG 7 cut(s) 133, 163, 338, 443, 1199, 1838, 1962
SmoI CTYRAG 7 cut(s) 133, 163, 338, 443, 1199, 1838, 1962
Sse8387I CCTGCAGG 1 cut(s) 1177
SseBI AGGCCT 1 cut(s) 111
SsiI CCGC 1 cut(s) 1027
SspI AATATT 1 cut(s) 1486
SspMI CTAG 1 cut(s) 1268
StuI AGGCCT 1 cut(s) 111
StyD4I CCNGG 3 cut(s) 1474, 1749, 1864
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 4 cut(s) 673, 761, 1559, 1826
TaiI ACGT 2 cut(s) 550, 1061
TaqI TCGA 5 cut(s) 144, 444, 567, 853, 1183
TatI WGTACW 2 cut(s) 1289, 1594
TfiI GAWTC 7 cut(s) 334, 378, 421, 473, 520, 1141, 1437
TscAI CASTG 3 cut(s) 1302, 1620, 1990
TseFI GTSAC 1 cut(s) 1132
Tsp45I GTSAC 1 cut(s) 1132
TspDTI ATGAA 8 cut(s) 210, 265, 839, 1345, 1398, 1414, 1512, 1650
TspGWI ACGGA 1 cut(s) 1883
TspRI CASTG 3 cut(s) 1302, 1620, 1990
Van91I CCANNNNNTGG 1 cut(s) 1615
Vha464I CTTAAG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 97
VspI ATTAAT 1 cut(s) 488
XapI RAATTY 6 cut(s) 182, 256, 1109, 1321, 1637, 1742
XceI RCATGY 1 cut(s) 833
XcmI CCANNNNNNNNNTGG 2 cut(s) 389, 1511
XhoI CTCGAG 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 1264
XmnI GAANNNNTTC 1 cut(s) 1458
XspI CTAG 1 cut(s) 1268
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.