RchiOBHm_Chr1g0322491

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
9851257 .. 9857626
6370 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55248

Sequence Viewer

Length: 3579 bp
ATGGCGTCAACAGCTTCTCGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGGGGGGAAGATACGCGCAGGGGTTTTACCGAGTATCTGTATACTGCACTAAATGAGAAAGGAATACTCACCTTTCTGGATGACCGAGACCTTGAGAAAGGAAAATCCATTAGTCCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATATGCGATTGTCATTCTCTCACACAGCTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATAAAAGAGATGGGCCAACAAGTCTTCCCCGTCTTCTACAAGGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAATGGGAAGCCCAAGTACAAGTAAGGGAACACGAAGAAGTTTATGGGAGGAATGAGGACAGACTAAATGCGTGGACGGCTGCTTTGACTAAGGTCGCCAATCTTTCTGGCTGGGATTACAAGAATTTTTCACATGAAGCAAAACTAATTGACGAAATTGTTGATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTATTAAGCAATTACATATGTCCTCAGCTTCGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGAATAGGTAAGACAACAGTTGCTAGAGCAATCCATGATGAAATTTGTCAAAATTTTAACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCATTGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGACAAAAATTGAAAATATAGAGGATGAATACATAGGAGCTGCTATGATAAAAAGGCGGTCGTGTAGAATGAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACGCAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATTATCGTAACCACCACAGATATTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTAATGAAGCACTTCAACTTTTGAGCTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAGAATTATTTGCATCTGTGCTACCATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATCTGGTAGAGGGGCTGATAAATGGGCAAGTGCAATAGATAGGCTAAGGAGCACACCAGATAAACGCATTATTGACGTGCTTCGGATTAGTTTTGATGACCTGCATGAAAAAGAGAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGCAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGAACCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTGAAGAGCCAGGCAAACGTAGTAGATTATGGTCTTATAAAGACATCAACAAGGTGCTGGAGAGAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTCAATTGCAAGTGGCTCATTGGAAGCCAGGAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCTCTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGAGCTCTCCCAAAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGCATCTTGAGACTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATCAATCCGTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCAAGAGTCTGCCAAGTAAAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCTGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTTTCTTGATGAGACTGCTATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTAGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTTTGTCTCCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTTGGCAGACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAATTGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCACAGAAAATATGTGAGAGGTTCAATCTTTCATGGATGCAAAGTAATTTGGCGATCTTTAAATAAGTTCTTGCCTTCTGGATTGGTGACAAAAGTGAATACAGAGCCAATGAGTTTCCACTTGCCTATATCTGGTCTGTCTAATTTAACATATCTGAACCTGAGTAATTCCAATCTTGGTGAAGGAGCATTTGCCAATGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTACACTTGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGACTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAAGAGTGAAACTTTCCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCGGACTGTTGGGTGTTCCTCAAGTGTATATCTACCTGCACATTGGAATAACAGTCGACTTATGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATAAGCACCATCGGGTGGATAAGCTTTGTAGAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAAAATTGGAAGTATATGGCAAACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCAAAACAGTTGCAGTCAGCTTGAGTTCTTATTTAAAACCAGAGGGCCGGGTCTAAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTAGGATATCTCCTTATGAGGATATATTGATTGGTTTTGACATTCCGGTTGCAGGGGAAACCAGTGGCACTGGTGGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1192

Amino Acids

135.42

Weight (kDa)

7.15

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 189 1.8e-54 TIR domain
TIR_2 PF13676 14 - 117 4.6e-16 TIR domain
NB-ARC PF00931 215 - 369 1.3e-16 NB-ARC domain
NACHT PF05729 219 - 315 8.5e-06 NACHT domain
WHD_ROQ1 PF23282 437 - 507 2.7e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 696 - 780 1.5e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 707 - 776 1.1e-08 Leucine-rich repeat region
C-JID PF20160 999 - 1148 9e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1560
AarI CACCTGC 1 cut(s) 1928
Acc36I ACCTGC 4 cut(s) 749, 1311, 1928, 3073
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1615, 3139
AccI GTMKAC 3 cut(s) 95, 554, 3085
AccII CGCG 1 cut(s) 70
AciI CCGC 4 cut(s) 340, 598, 673, 880
AclI AACGTT 1 cut(s) 2161
AclWI GGATC 6 cut(s) 320, 333, 655, 668, 930, 1374
AcuI CTGAAG 4 cut(s) 1545, 2682, 2960, 3224
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 384, 2788, 2815, 3120, 3331
AfiI CCNNNNNNNGG 5 cut(s) 1412, 1615, 2564, 3139, 3527
AflII CTTAAG 1 cut(s) 1777
AjiI CACGTC 1 cut(s) 1279
AjnI CCWGG 5 cut(s) 973, 1531, 1657, 2998, 3273
AjuI GAANNNNNNNTTGG 6 cut(s) 1395, 1427, 2720, 2752, 2979, 3011
Alw21I GWGCWC 3 cut(s) 366, 1256, 1786
AlwI GGATC 6 cut(s) 320, 333, 655, 668, 930, 1374
AoxI GGCC 3 cut(s) 292, 1723, 3386
ApeKI GCWGC 3 cut(s) 182, 446, 863
ArsI GACNNNNNNTTYG 2 cut(s) 433, 465
AseI ATTAAT 1 cut(s) 2102
Asp700I GAANNNNTTC 6 cut(s) 592, 804, 1074, 1506, 1600, 2945
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 4 cut(s) 292, 971, 1711, 3386
AsuC2I CCSGG 1 cut(s) 3390
AsuHPI GGTGA 6 cut(s) 115, 2530, 2624, 2668, 3281, 3412
AvaII GGWCC 2 cut(s) 971, 1711
BamHI GGATCC 2 cut(s) 325, 660
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 2 cut(s) 366, 1786
BarI GAAGNNNNNNTAC 2 cut(s) 366, 398
BauI CACGAG 2 cut(s) 1181, 3312
BbsI GAAGAC 4 cut(s) 295, 304, 1682, 2948
Bbv12I GWGCWC 3 cut(s) 366, 1256, 1786
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 3 cut(s) 169, 433, 850
BccI CCATC 9 cut(s) 283, 2004, 2294, 2362, 2785, 2801, 3036, 3141, 3206
BceAI ACGGC 2 cut(s) 459, 2237
BciT130I CCWGG 5 cut(s) 975, 1533, 1659, 3000, 3275
BciVI GTATCC 1 cut(s) 1782
BclI TGATCA 2 cut(s) 529, 2431
BcnI CCSGG 1 cut(s) 3390
BfaI CTAG 5 cut(s) 198, 699, 1385, 1400, 2229
BfmI CTRYAG 1 cut(s) 2257
BfrI CTTAAG 1 cut(s) 1777
BfuAI ACCTGC 4 cut(s) 749, 1311, 1928, 3073
BfuI GTATCC 1 cut(s) 1782
BglII AGATCT 2 cut(s) 1436, 1963
BisI GCNGC 4 cut(s) 183, 341, 447, 864
BlsI GCNGC 4 cut(s) 184, 342, 448, 865
Bme1390I CCNGG 6 cut(s) 975, 1533, 1659, 3000, 3275, 3390
Bme18I GGWCC 2 cut(s) 971, 1711
BmgBI CACGTC 1 cut(s) 1279
BmgT120I GGNCC 4 cut(s) 292, 971, 1711, 3386
BmiI GGNNCC 4 cut(s) 327, 344, 662, 1663
BmrFI CCNGG 6 cut(s) 975, 1533, 1659, 3000, 3275, 3390
BmrI ACTGGG 1 cut(s) 1776
BmsI GCATC 9 cut(s) 1024, 1141, 1461, 1957, 2318, 2459, 2827, 2849, 2903
BmuI ACTGGG 1 cut(s) 1776
BoxI GACNNNNGTC 1 cut(s) 458
BpiI GAAGAC 4 cut(s) 295, 304, 1682, 2948
BpmI CTGGAG 1 cut(s) 1601
Bpu10I CCTNAGC 3 cut(s) 633, 1161, 1247
BpuEI CTTGAG 7 cut(s) 167, 1768, 1973, 2042, 2744, 3034, 3383
BpuMI CCSGG 1 cut(s) 3390
BsaBI GATNNNNATC 2 cut(s) 921, 1365
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 135, 1916
BsaJI CCNNGG 3 cut(s) 664, 974, 1609
BsaWI WCCGGW 1 cut(s) 3520
Bsc4I CCNNNNNNNGG 5 cut(s) 1412, 1615, 2564, 3139, 3527
Bse1I ACTGG 4 cut(s) 968, 1771, 3537, 3550
Bse8I GATNNNNATC 2 cut(s) 921, 1365
BseBI CCWGG 5 cut(s) 975, 1533, 1659, 3000, 3275
BseDI CCNNGG 3 cut(s) 664, 974, 1609
BseGI GGATG 7 cut(s) 139, 259, 853, 1979, 2333, 2474, 2918
BseJI GATNNNNATC 2 cut(s) 921, 1365
BseLI CCNNNNNNNGG 5 cut(s) 1412, 1615, 2564, 3139, 3527
BseMII CTCAG 5 cut(s) 647, 1175, 2130, 2585, 3006
BseNI ACTGG 4 cut(s) 968, 1771, 3537, 3550
BseRI GAGGAG 2 cut(s) 206, 1745
BseXI GCAGC 3 cut(s) 169, 433, 850
BseYI CCCAGC 2 cut(s) 477, 1497
BsgI GTGCAG 2 cut(s) 84, 3051
Bsh1236I CGCG 1 cut(s) 70
Bsh1285I CGRYCG 1 cut(s) 884
BshFI GGCC 3 cut(s) 294, 1725, 3388
BshNI GGYRCC 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 884
BsiHKAI GWGCWC 3 cut(s) 366, 1256, 1786
BsiSI CCGG 2 cut(s) 3389, 3521
BslFI GGGAC 1 cut(s) 1095
BslI CCNNNNNNNGG 5 cut(s) 1412, 1615, 2564, 3139, 3527
BsmFI GGGAC 1 cut(s) 1095
BsnI GGCC 3 cut(s) 294, 1725, 3388
Bso31I GGTCTC 2 cut(s) 135, 1916
Bsp1286I GDGCHC 3 cut(s) 366, 1256, 1786
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 4 cut(s) 340, 598, 673, 880
BspANI GGCC 3 cut(s) 294, 1725, 3388
BspCNI CTCAG 5 cut(s) 646, 1174, 2131, 2586, 3007
BspFNI CGCG 1 cut(s) 70
BspLI GGNNCC 4 cut(s) 327, 344, 662, 1663
BspMAI CTGCAG 1 cut(s) 2261
BspMI ACCTGC 4 cut(s) 749, 1311, 1928, 3073
BspPI GGATC 6 cut(s) 320, 333, 655, 668, 930, 1374
BspQI GCTCTTC 1 cut(s) 1521
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 1777
BspTNI GGTCTC 2 cut(s) 135, 1916
BsrI ACTGG 4 cut(s) 968, 1771, 3537, 3550
BssECI CCNNGG 3 cut(s) 664, 974, 1609
BssNAI GTATAC 2 cut(s) 96, 555
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1181, 3312
BssT1I CCWWGG 2 cut(s) 664, 1609
Bst1107I GTATAC 2 cut(s) 96, 555
Bst2BI CACGAG 2 cut(s) 1181, 3312
Bst2UI CCWGG 5 cut(s) 975, 1533, 1659, 3000, 3275
Bst4CI ACNGT 8 cut(s) 694, 1064, 1467, 1518, 2375, 3037, 3083, 3350
Bst6I CTCTTC 1 cut(s) 1521
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1777
BstAPI GCANNNNNTGC 3 cut(s) 1480, 3439, 3558
BstC8I GCNNGC 5 cut(s) 671, 1342, 1397, 3246, 3563
BstDSI CCRYGG 1 cut(s) 664
BstEII GGTNACC 1 cut(s) 2656
BstF5I GGATG 7 cut(s) 139, 259, 853, 1979, 2333, 2474, 2918
BstFNI CGCG 1 cut(s) 70
BstHHI GCGC 1 cut(s) 72
BstMCI CGRYCG 1 cut(s) 884
BstMWI GCNNNNNNNGC 8 cut(s) 11, 443, 1480, 2719, 3107, 3439, 3549, 3558
BstNI CCWGG 5 cut(s) 975, 1533, 1659, 3000, 3275
BstNSI RCATGY 1 cut(s) 673
BstPAI GACNNNNGTC 1 cut(s) 458
BstPI GGTNACC 1 cut(s) 2656
BstSCI CCNGG 6 cut(s) 973, 1531, 1657, 2998, 3273, 3388
BstSFI CTRYAG 1 cut(s) 2257
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 3 cut(s) 169, 433, 850
BstV2I GAAGAC 4 cut(s) 295, 304, 1682, 2948
BstX2I RGATCY 4 cut(s) 325, 660, 1436, 1963
BstYI RGATCY 4 cut(s) 325, 660, 1436, 1963
BstZ17I GTATAC 2 cut(s) 96, 555
BsuI GTATCC 1 cut(s) 1782
BsuRI GGCC 3 cut(s) 294, 1725, 3388
BtgI CCRYGG 1 cut(s) 664
BtrI CACGTC 1 cut(s) 1279
BtsCI GGATG 7 cut(s) 139, 259, 853, 1979, 2333, 2474, 2918
BtsIMutI CAGTG 3 cut(s) 2819, 3543, 3544
BveI ACCTGC 4 cut(s) 749, 1311, 1928, 3073
Cac8I GCNNGC 5 cut(s) 671, 1342, 1397, 3246, 3563
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 4 cut(s) 292, 971, 1711, 3386
CseI GACGC 1 cut(s) 940
Csp6I GTAC 5 cut(s) 383, 2787, 2814, 3119, 3330
CviQI GTAC 5 cut(s) 383, 2787, 2814, 3119, 3330
DraI TTTAAA 2 cut(s) 2493, 3376
Eam1104I CTCTTC 1 cut(s) 1521
EarI CTCTTC 1 cut(s) 1521
Ecl136II GAGCTC 2 cut(s) 364, 1784
Eco130I CCWWGG 2 cut(s) 664, 1609
Eco147I AGGCCT 1 cut(s) 1725
Eco24I GRGCYC 2 cut(s) 366, 1786
Eco31I GGTCTC 2 cut(s) 135, 1916
Eco32I GATATC 1 cut(s) 3483
Eco47I GGWCC 2 cut(s) 971, 1711
Eco53kI GAGCTC 2 cut(s) 364, 1784
Eco57I CTGAAG 4 cut(s) 1545, 2682, 2960, 3224
Eco91I GGTNACC 1 cut(s) 2656
EcoICRI GAGCTC 2 cut(s) 364, 1784
EcoO65I GGTNACC 1 cut(s) 2656
EcoRI GAATTC 1 cut(s) 3161
EcoRII CCWGG 5 cut(s) 973, 1531, 1657, 2998, 3273
EcoRV GATATC 1 cut(s) 3483
EcoT14I CCWWGG 2 cut(s) 664, 1609
EcoT22I ATGCAT 3 cut(s) 281, 1476, 2842
EcoT38I GRGCYC 2 cut(s) 366, 1786
ErhI CCWWGG 2 cut(s) 664, 1609
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 1 cut(s) 1095
FauI CCCGC 2 cut(s) 605, 666
FauNDI CATATG 1 cut(s) 626
FbaI TGATCA 2 cut(s) 529, 2431
FblI GTMKAC 3 cut(s) 95, 554, 3085
Fnu4HI GCNGC 4 cut(s) 183, 341, 447, 864
FokI GGATG 7 cut(s) 146, 266, 860, 1986, 2340, 2481, 2925
FriOI GRGCYC 2 cut(s) 366, 1786
Fsp4HI GCNGC 4 cut(s) 183, 341, 447, 864
FspBI CTAG 5 cut(s) 198, 699, 1385, 1400, 2229
GlaI GCGC 1 cut(s) 71
GluI GCNGC 4 cut(s) 183, 341, 447, 864
GsaI CCCAGC 2 cut(s) 481, 1501
GsuI CTGGAG 1 cut(s) 1601
HaeIII GGCC 3 cut(s) 294, 1725, 3388
HapII CCGG 2 cut(s) 3389, 3521
HgaI GACGC 1 cut(s) 940
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 5 cut(s) 9, 571, 930, 1629, 3086
HindII GTYRAC 5 cut(s) 9, 571, 930, 1629, 3086
HindIII AAGCTT 3 cut(s) 2021, 2720, 3146
HpaII CCGG 2 cut(s) 3389, 3521
HphI GGTGA 6 cut(s) 115, 2530, 2624, 2668, 3281, 3412
Hpy188I TCNGA 7 cut(s) 334, 1287, 2589, 2770, 3033, 3286, 3423
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 8 cut(s) 694, 1064, 1467, 1518, 2375, 3037, 3083, 3350
HpyCH4IV ACGT 3 cut(s) 1278, 1540, 2161
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 443, 1480, 2719, 3107, 3439, 3549, 3558
HpySE526I ACGT 3 cut(s) 1278, 1540, 2161
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 70
Ksp22I TGATCA 2 cut(s) 529, 2431
LguI GCTCTTC 1 cut(s) 1521
LmnI GCTCC 4 cut(s) 860, 1251, 1661, 2618
Lsp1109I GCAGC 3 cut(s) 169, 433, 850
LweI GCATC 9 cut(s) 1024, 1141, 1461, 1957, 2318, 2459, 2827, 2849, 2903
MaeI CTAG 5 cut(s) 198, 699, 1385, 1400, 2229
MaeII ACGT 3 cut(s) 1278, 1540, 2161
MaeIII GTNAC 5 cut(s) 991, 1372, 2518, 2639, 2656
MfeI CAATTG 4 cut(s) 186, 1634, 2214, 2884
MflI RGATCY 4 cut(s) 325, 660, 1436, 1963
MhlI GDGCHC 3 cut(s) 366, 1256, 1786
MlyI GAGTC 5 cut(s) 203, 1624, 1746, 2071, 3423
MmeI TCCRAC 3 cut(s) 1479, 1987, 2128
Mph1103I ATGCAT 3 cut(s) 281, 1476, 2842
MroXI GAANNNNTTC 6 cut(s) 592, 804, 1074, 1506, 1600, 2945
MslI CAYNNNNRTG 3 cut(s) 228, 587, 1619
MspCI CTTAAG 1 cut(s) 1777
MspI CCGG 2 cut(s) 3389, 3521
MspR9I CCNGG 6 cut(s) 975, 1533, 1659, 3000, 3275, 3390
MunI CAATTG 4 cut(s) 186, 1634, 2214, 2884
MvaI CCWGG 5 cut(s) 975, 1533, 1659, 3000, 3275
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 8 cut(s) 11, 443, 1480, 2719, 3107, 3439, 3549, 3558
NciI CCSGG 1 cut(s) 3390
NcoI CCATGG 1 cut(s) 664
NdeI CATATG 1 cut(s) 626
NlaIV GGNNCC 4 cut(s) 327, 344, 662, 1663
NmuCI GTSAC 3 cut(s) 1372, 2518, 2656
NsiI ATGCAT 3 cut(s) 281, 1476, 2842
NspI RCATGY 1 cut(s) 673
PaeI GCATGC 1 cut(s) 673
PaqCI CACCTGC 1 cut(s) 1928
PceI AGGCCT 1 cut(s) 1725
PciSI GCTCTTC 1 cut(s) 1521
PcsI WCGNNNNNNNCGW 1 cut(s) 646
PdmI GAANNNNTTC 6 cut(s) 592, 804, 1074, 1506, 1600, 2945
PflMI CCANNNNNTGG 2 cut(s) 1615, 3139
PfoI TCCNGGA 1 cut(s) 2998
PkrI GCNGC 4 cut(s) 184, 342, 448, 865
PleI GAGTC 5 cut(s) 202, 1624, 1746, 2070, 3422
PpsI GAGTC 5 cut(s) 202, 1624, 1746, 2070, 3422
PshAI GACNNNNGTC 1 cut(s) 458
PshBI ATTAAT 1 cut(s) 2102
PsiI TTATAA 1 cut(s) 1560
Psp124BI GAGCTC 2 cut(s) 366, 1786
Psp1406I AACGTT 1 cut(s) 2161
Psp6I CCWGG 5 cut(s) 973, 1531, 1657, 2998, 3273
PspEI GGTNACC 1 cut(s) 2656
PspFI CCCAGC 2 cut(s) 477, 1497
PspGI CCWGG 5 cut(s) 973, 1531, 1657, 2998, 3273
PspN4I GGNNCC 4 cut(s) 327, 344, 662, 1663
PspPI GGNCC 4 cut(s) 292, 971, 1711, 3386
PstI CTGCAG 1 cut(s) 2261
PsuI RGATCY 4 cut(s) 325, 660, 1436, 1963
RsaI GTAC 5 cut(s) 384, 2788, 2815, 3120, 3331
RsaNI GTAC 5 cut(s) 383, 2787, 2814, 3119, 3330
RseI CAYNNNNRTG 3 cut(s) 228, 587, 1619
SacI GAGCTC 2 cut(s) 366, 1786
SalI GTCGAC 1 cut(s) 3084
SapI GCTCTTC 1 cut(s) 1521
SatI GCNGC 4 cut(s) 183, 341, 447, 864
Sau96I GGNCC 4 cut(s) 292, 971, 1711, 3386
SchI GAGTC 5 cut(s) 203, 1624, 1746, 2071, 3423
ScrFI CCNGG 6 cut(s) 975, 1533, 1659, 3000, 3275, 3390
SduI GDGCHC 3 cut(s) 366, 1256, 1786
SfaNI GCATC 9 cut(s) 1024, 1141, 1461, 1957, 2318, 2459, 2827, 2849, 2903
SfcI CTRYAG 1 cut(s) 2257
SinI GGWCC 2 cut(s) 971, 1711
SmiMI CAYNNNNRTG 3 cut(s) 228, 587, 1619
SmlI CTYRAG 8 cut(s) 146, 1747, 1777, 1952, 2057, 2723, 3049, 3362
SmoI CTYRAG 8 cut(s) 146, 1747, 1777, 1952, 2057, 2723, 3049, 3362
SphI GCATGC 1 cut(s) 673
SseBI AGGCCT 1 cut(s) 1725
SsiI CCGC 4 cut(s) 340, 598, 673, 880
SspI AATATT 2 cut(s) 1326, 3010
SspMI CTAG 5 cut(s) 198, 699, 1385, 1400, 2229
SstI GAGCTC 2 cut(s) 366, 1786
StuI AGGCCT 1 cut(s) 1725
StyD4I CCNGG 6 cut(s) 973, 1531, 1657, 2998, 3273, 3388
StyI CCWWGG 2 cut(s) 664, 1609
TaaI ACNGT 8 cut(s) 694, 1064, 1467, 1518, 2375, 3037, 3083, 3350
TaiI ACGT 3 cut(s) 1281, 1543, 2164
TaqI TCGA 4 cut(s) 1469, 1758, 3085, 3210
TaqII GACCGA 1 cut(s) 153
TatI WGTACW 4 cut(s) 382, 2786, 2813, 3118
TauI GCSGC 1 cut(s) 343
TscAI CASTG 3 cut(s) 2826, 3544, 3550
TseFI GTSAC 3 cut(s) 1372, 2518, 2656
TseI GCWGC 3 cut(s) 182, 446, 863
Tsp45I GTSAC 3 cut(s) 1372, 2518, 2656
TspGWI ACGGA 4 cut(s) 538, 1161, 1993, 3407
TspRI CASTG 3 cut(s) 2826, 3544, 3550
Van91I CCANNNNNTGG 2 cut(s) 1615, 3139
Vha464I CTTAAG 1 cut(s) 1777
VpaK11BI GGWCC 2 cut(s) 971, 1711
VspI ATTAAT 1 cut(s) 2102
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 673
XcmI CCANNNNNNNNNTGG 2 cut(s) 2003, 3035
XmiI GTMKAC 3 cut(s) 95, 554, 3085
XmnI GAANNNNTTC 6 cut(s) 592, 804, 1074, 1506, 1600, 2945
XspI CTAG 5 cut(s) 198, 699, 1385, 1400, 2229
Zsp2I ATGCAT 3 cut(s) 281, 1476, 2842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.