Rroxscaffold_7G00217130

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000105
Physical Location & Seq
Reverse (-)
67655981 .. 67661256
5276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00217130.1

Sequence Viewer

Length: 3576 bp
ATGGCGTCAACTTGGTCTCCTCCTCGGCGATGGAAATACGATGTGTTTCTAAGCTTCAGAGGGGAAGATACCCGCGAGACTTTCATCTGCCATCTGTATCGTGCACTAACTGCGAAAGGAATTATAACCTTTTTAGATGACGAAAGCCTGGAGAGAGGATCAACCATTGGCCCATCGCTTTTTACTGCAATTCAGGACTCAAGAATTGCCCTTGTCGTTCTTTCACAGAATTTCGCTTCTTCATCCTGGTGCTTGGATGAACTCTCCAAGATTCTTCGATCCATGGAAGAAAGGGGACGCGTCATCCCGATCTTCTACAAAGTGGATCCTTCTGATGTGCGCAAGCAAAGGGGAAGTTTTCAGCTCCGTACGGTAAAGGAACATGAAGAAGTTTATGACAAACGAAAGGAGTGGACAGATGCTTTGACAAAGGTGGCCAATCTTGTTGGGATTCATTCGAGTGGCCAGAAGGATGACGGAAAATTGATTGAGCTCATTGTTACGGAGACACTAATGTACCTAGAATTTATTTCTACCTCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTATCAAGCAATTACATACGTCCACAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCCCGAGCCATTTATGATGAACTGTCTCGCAGGTTTGACAGAAGCTGCATTCTTTACAATGTTAGAGAAAAATCTGAAAAGGATGGCCTTGTTTCTCTACAAGAAAAACTACTTTCCAGAATCCTGAGGACAAAAGTTGACATTGAGGATGAATATGCAGGAGCTACTATGATAGAAAGGCAGTTATGCAAACTAAAGGTGCTTGTTGTTATTGATGATGTGGATCACATCAATCAATTAGATAAATTGGCTGGAAGTCGGGACTGGTTCGGTCCGGGGAGTAGAATTATTGTAACCACACCAGATATTCATTTGCTAAGGGGACATGATGTTGATGCTACATACAAGGCTACTGGGTTAGCTCGTGGTGAAGCTGTTCAGCTATTGAGTTTAAAGGCTTTCAAGAAAAGCTTCCCACCAGAAGATTATTTGGACTTGTGCCACCAGATTATAAGGTATGCTCAGGGGCTTCCGTTGGCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGACCCGTTGAATGGGTAAGTACAATAAAGAGGCTAAGCAATACACCAAATAGACAAGTTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGAAAAAGACAAACAGATATTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGGCTATTGCAAGCTAGAACCTGGCATTGGTTTAAAGGTTCTTGAGGAAAAATCTCTCATTATTATTTTTAACAAAAAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTTGGAAATAGTTCGTCGACAGTCCCCTGAAGAGCCAGGTAGACGAAGTAGATTATGGTCTCCTGAAAATATCCACAATGTGCTGAAGAAAAATAAGGGAACAGACAAAATCCAAGCCATGGTAATGGACTTGCCTGAGTTAGAAGTGGCTCATTGGAAACCAGACGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCGTAATGTGGATCTTCCCGAAGGCCTCACTTTTCTATCTAATTCCTTGAGACTCCTTGAATGGAATGGGTACCCCTTAAGATCCCTCCCACAAAATTTTGAACCAGACGAACTTATTGAACTTAACTTGTGCCACAGCAACATTGAACACCTCTGGAAAGGAGCAAAGAATTTTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCAGACCCCAGACCTCAGAGGCGTTCAAAATCTTGAGACTTTAGATCTTGAAGGATGTAAGACTTTGGTAAAACTTCATGAATCCCTGGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAAGATTGCGAGAGTCTCGAGAGTCTGCCAAGTAAGATTGAAATGGAATCTCTTCAAACTTTAATTCTTTCTAACTGCTCAAAAGTTAAAAGGATTCCTGAGTTCGCTGGAAATATGGGGCGTTTATCAGAGCTTTATCTCGATGAGACTGCCATTGAAAAACTACCTGTGTCAATTGGATGCCTGAGTGGCCTTACTTCGCTGAATCTGAGCAACTGCAGAAATCTTGCTTGCCTTCCAAGCACCTTTTGTAGATTGGAGTCTCTTGAAAAGCTTAATCTTTCTGGATGTTTGAAACTTGGAGAACTGGATAGTGCAAGGGAGAATGGTGCAATTAGAACTGCTCTAAATCATCTTACAGAATGCTTTTGTGAAGTTGTTGGGAGATATTTGTCATGGTCCTTGCCTTCTAGATTGGTGCAAAGAGCCGATATAGAACCAATGAGTATGCAGTTGCCTATATCTGATCTGTGTTATTCTACAGAGCAAAGTGCAAATACATCAATGAGTATCCAGTTGCCGCTATCTCGTCTATGTAATTTAACGGATCTAAACATAAGTAACTGCAATCTTGGTGACGTTGCATTTGCCAACTTTGCCTGTTTTCCCTCCTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTAAACTTCAGAACATTAATTTGGAGAATTGCAAGACACTTCAAGAATTGTCAGGACTTCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAAATGCTGTTTGATGTATCCAATTTCAATAGATTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAGTCAGGACTGCAGAAACATAGCACTTGAAATGCTGAGGATATTCCTTAATCAGCAAATTTCTAGTGCGATGGAAACTTTTCAAATTGTAATTCCTGGAAGTAGAATTCCTGAGTGGTTTGATCGTCGAAGATTTGGGAGTTCAGTAACTGTAAACCCACCTCGGTGTAGGGGTAGGATTTTGGGATTTGTTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCAGGTTGATGAGCTTAATATCTGTCTGTTCAAGACTTTTAAGGCTAGTCATCATCTTGTATGTCGCCTGAAGCTCAATGGAAATGAATTAGAAGCATCTGGCAAACAGCCTGCATTTCGCTTTAGCGAACAATTTTGCCAGGTTGAGTCAGATCACCTTTGGATATTCTATGTGTCCCATGATAAATACTTTGGTACAGATTGGTGGCATAAAAGTTGCAGTCAGCTTGAGTTCTTATTTGAAACAAGAGGGCCAGGTCTGCAGGTGAAGGAGTGTGGGGTACGTCTGATATATGAGCAAGATGTGCTAGAGTTGAACCAAACAATAACTCAATCAAGCAGTAGGATGTCTCCTCCATTCACCCAAAAAAAAAGTCGAAAGGGGAAACCAGTGGTACTGGTAGCAGAACCTGCACCCTCGAAGAACTATAGGATTGGCAGAAAAAGGAGGATTCAATACGGGCCACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1191

Amino Acids

135.47

Weight (kDa)

8.38

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 181 1.5e-49 TIR domain
TIR_2 PF13676 15 - 125 1.3e-15 TIR domain
NB-ARC PF00931 208 - 362 6.2e-17 NB-ARC domain
AAA_22 PF13401 210 - 308 2.9e-07 AAA domain
NACHT PF05729 212 - 308 2.2e-06 NACHT domain
WHD_ROQ1 PF23282 428 - 499 5.7e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 628 - 882 1.3e-09 Leucine-rich repeat region
LRR_13 PF23286 688 - 774 4.7e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 991 - 1137 3.2e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 125, 1127
AarI CACCTGC 1 cut(s) 3358
Acc16I TGCGCA 1 cut(s) 341
Acc36I ACCTGC 3 cut(s) 696, 3358, 3523
Acc65I GGTACC 1 cut(s) 1746
AccB1I GGYRCC 1 cut(s) 1746
AccB7I CCANNNNNTGG 2 cut(s) 617, 1591
AccI GTMKAC 2 cut(s) 1489, 1513
AccII CGCG 2 cut(s) 75, 300
AciI CCGC 4 cut(s) 73, 577, 652, 2537
AcoI YGGCCR 2 cut(s) 435, 463
AcsI RAATTY 7 cut(s) 229, 524, 1648, 1771, 1846, 2937, 2985
AcuI CTGAAG 6 cut(s) 40, 1244, 1521, 1577, 2684, 3194
AcyI GRCGYC 2 cut(s) 5, 1638
AdeI CACNNNGTG 1 cut(s) 1552
AfaI GTAC 9 cut(s) 370, 518, 1210, 1325, 1748, 3090, 3301, 3387, 3501
AfiI CCNNNNNNNGG 4 cut(s) 617, 1200, 1388, 1591
AflII CTTAAG 1 cut(s) 1753
AflIII ACRYGT 1 cut(s) 298
AjnI CCWGG 8 cut(s) 147, 245, 1381, 1507, 1980, 2974, 3243, 3358
AjuI GAANNNNNNNTTGG 4 cut(s) 1371, 1403, 2696, 2728
AleI CACNNNNGTG 1 cut(s) 3043
Alw21I GWGCWC 3 cut(s) 106, 495, 3102
Alw44I GTGCAC 1 cut(s) 102
AlwNI CAGNNNCTG 4 cut(s) 720, 2688, 3029, 3515
Ama87I CYCGRG 2 cut(s) 678, 2033
AoxI GGCC 8 cut(s) 169, 435, 463, 760, 1699, 2206, 3356, 3566
ApaLI GTGCAC 1 cut(s) 102
ApeKI GCWGC 1 cut(s) 720
ApoI RAATTY 7 cut(s) 229, 524, 1648, 1771, 1846, 2937, 2985
ArsI GACNNNNNNTTYG 6 cut(s) 406, 438, 2585, 2617, 3463, 3495
AseI ATTAAT 1 cut(s) 2709
Asp700I GAANNNNTTC 6 cut(s) 571, 1050, 1085, 1482, 2067, 2958
Asp718I GGTACC 1 cut(s) 1746
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 6 cut(s) 170, 947, 2415, 2685, 3356, 3566
AsuC2I CCSGG 1 cut(s) 951
AsuHPI GGTGA 5 cut(s) 1055, 2603, 3251, 3382, 3457
AvaI CYCGRG 2 cut(s) 678, 2033
AvaII GGWCC 3 cut(s) 947, 2415, 2685
AxyI CCTNAGG 1 cut(s) 800
BaeGI GKGCMC 1 cut(s) 106
BaeI ACNNNNGTAYC 2 cut(s) 500, 533
BalI TGGCCA 2 cut(s) 437, 465
BamHI GGATCC 2 cut(s) 325, 639
BanI GGYRCC 1 cut(s) 1746
BanII GRGCYC 1 cut(s) 495
BauI CACGAG 1 cut(s) 1038
BbsI GAAGAC 2 cut(s) 1195, 1658
Bbv12I GWGCWC 3 cut(s) 106, 495, 3102
BbvCI CCTCAGC 1 cut(s) 2915
BbvI GCAGC 1 cut(s) 707
BccI CCATC 9 cut(s) 24, 99, 181, 752, 1268, 2761, 2777, 2944, 3111
BcgI CGANNNNNNTGC 2 cut(s) 2147, 2181
BciT130I CCWGG 8 cut(s) 149, 247, 1383, 1509, 1982, 2976, 3245, 3360
BciVI GTATCC 2 cut(s) 2537, 2827
BcnI CCSGG 1 cut(s) 951
BfaI CTAG 8 cut(s) 521, 1280, 1361, 1376, 2427, 2943, 3150, 3413
BfmI CTRYAG 5 cut(s) 2233, 2496, 2890, 3365, 3532
BfrI CTTAAG 1 cut(s) 1753
BfuAI ACCTGC 3 cut(s) 696, 3358, 3523
BfuI GTATCC 2 cut(s) 2537, 2827
BglI GCCNNNNNGGC 1 cut(s) 2205
BglII AGATCT 1 cut(s) 1939
BisI GCNGC 2 cut(s) 721, 2537
BlpI GCTNAGC 1 cut(s) 1223
BlsI GCNGC 2 cut(s) 722, 2538
Bme1390I CCNGG 9 cut(s) 149, 247, 951, 1383, 1509, 1982, 2976, 3245, 3360
Bme18I GGWCC 3 cut(s) 947, 2415, 2685
BmeT110I CYCGRG 2 cut(s) 678, 2033
BmgT120I GGNCC 6 cut(s) 170, 947, 2415, 2685, 3356, 3566
BmiI GGNNCC 3 cut(s) 327, 641, 1748
BmrFI CCNGG 9 cut(s) 149, 247, 951, 1383, 1509, 1982, 2976, 3245, 3360
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 6 cut(s) 409, 1000, 1437, 2186, 2688, 3209
BmuI ACTGGG 1 cut(s) 1038
BpiI GAAGAC 2 cut(s) 1195, 1658
BpmI CTGGAG 1 cut(s) 170
Bpu10I CCTNAGC 3 cut(s) 992, 1137, 2915
Bpu1102I GCTNAGC 1 cut(s) 1223
BpuEI CTTGAG 5 cut(s) 184, 1424, 1744, 1949, 3353
BpuMI CCSGG 1 cut(s) 951
BsaBI GATNNNNATC 1 cut(s) 897
BsaHI GRCGYC 2 cut(s) 5, 1638
BsaI GGTCTC 2 cut(s) 21, 1536
BsaJI CCNNGG 8 cut(s) 23, 282, 643, 950, 1590, 1980, 1981, 3041
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 4 cut(s) 617, 1200, 1388, 1591
Bse1I ACTGG 7 cut(s) 944, 1033, 2328, 2530, 2802, 3494, 3507
Bse21I CCTNAGG 1 cut(s) 800
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 8 cut(s) 149, 247, 1383, 1509, 1982, 2976, 3245, 3360
BseDI CCNNGG 8 cut(s) 23, 282, 643, 950, 1590, 1980, 1981, 3041
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 4 cut(s) 617, 1200, 1388, 1591
BseNI ACTGG 7 cut(s) 944, 1033, 2328, 2530, 2802, 3494, 3507
BseRI GAGGAG 3 cut(s) 9, 12, 3447
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 1 cut(s) 707
BsgI GTGCAG 1 cut(s) 3501
Bsh1236I CGCG 2 cut(s) 75, 300
BshFI GGCC 8 cut(s) 171, 437, 465, 762, 1701, 2208, 3358, 3568
BshNI GGYRCC 1 cut(s) 1746
BsiHKAI GWGCWC 3 cut(s) 106, 495, 3102
BsiHKCI CYCGRG 2 cut(s) 678, 2033
BsiSI CCGG 1 cut(s) 950
BsiWI CGTACG 1 cut(s) 368
BslFI GGGAC 6 cut(s) 309, 950, 1011, 1480, 1998, 3265
BslI CCNNNNNNNGG 4 cut(s) 617, 1200, 1388, 1591
BsmFI GGGAC 6 cut(s) 309, 950, 1011, 1480, 1998, 3265
BsmI GAATGC 2 cut(s) 723, 2384
BsnI GGCC 8 cut(s) 171, 437, 465, 762, 1701, 2208, 3358, 3568
Bso31I GGTCTC 2 cut(s) 21, 1536
BsoBI CYCGRG 2 cut(s) 678, 2033
Bsp1286I GDGCHC 3 cut(s) 106, 495, 3102
Bsp1407I TGTACA 1 cut(s) 1323
Bsp1720I GCTNAGC 1 cut(s) 1223
Bsp19I CCATGG 3 cut(s) 282, 643, 1590
BspACI CCGC 4 cut(s) 73, 577, 652, 2537
BspANI GGCC 8 cut(s) 171, 437, 465, 762, 1701, 2208, 3358, 3568
BspFNI CGCG 2 cut(s) 75, 300
BspHI TCATGA 1 cut(s) 1972
BspLI GGNNCC 3 cut(s) 327, 641, 1748
BspMAI CTGCAG 3 cut(s) 2237, 2894, 3369
BspMI ACCTGC 3 cut(s) 696, 3358, 3523
BspQI GCTCTTC 1 cut(s) 1497
BspT107I GGYRCC 1 cut(s) 1746
BspTI CTTAAG 1 cut(s) 1753
BspTNI GGTCTC 2 cut(s) 21, 1536
BsrGI TGTACA 1 cut(s) 1323
BsrI ACTGG 7 cut(s) 944, 1033, 2328, 2530, 2802, 3494, 3507
BssECI CCNNGG 8 cut(s) 23, 282, 643, 950, 1590, 1980, 1981, 3041
BssNI GRCGYC 2 cut(s) 5, 1638
BssSI CACGAG 1 cut(s) 1038
BssT1I CCWWGG 3 cut(s) 282, 643, 1590
Bst2BI CACGAG 1 cut(s) 1038
Bst2UI CCWGG 8 cut(s) 149, 247, 1383, 1509, 1982, 2976, 3245, 3360
Bst4CI ACNGT 6 cut(s) 373, 699, 1443, 1494, 2882, 3031
Bst6I CTCTTC 3 cut(s) 544, 1497, 2073
BstACI GRCGYC 2 cut(s) 5, 1638
BstAFI CTTAAG 1 cut(s) 1753
BstAPI GCANNNNNTGC 4 cut(s) 110, 1456, 3409, 3515
BstAUI TGTACA 1 cut(s) 1323
BstC8I GCNNGC 5 cut(s) 344, 650, 1373, 2248, 3216
BstDSI CCRYGG 3 cut(s) 282, 643, 1590
BstFNI CGCG 2 cut(s) 75, 300
BstHHI GCGC 1 cut(s) 342
BstMWI GCNNNNNNNGC 9 cut(s) 110, 654, 1456, 2205, 2256, 2612, 3364, 3409, 3515
BstNI CCWGG 8 cut(s) 149, 247, 1383, 1509, 1982, 2976, 3245, 3360
BstNSI RCATGY 1 cut(s) 652
BstSCI CCNGG 9 cut(s) 147, 245, 949, 1381, 1507, 1980, 2974, 3243, 3358
BstSFI CTRYAG 5 cut(s) 2233, 2496, 2890, 3365, 3532
BstSLI GKGCMC 1 cut(s) 106
BstUI CGCG 2 cut(s) 75, 300
BstV1I GCAGC 1 cut(s) 707
BstV2I GAAGAC 2 cut(s) 1195, 1658
BstX2I RGATCY 6 cut(s) 325, 639, 1687, 1757, 1939, 2563
BstXI CCANNNNNNTGG 1 cut(s) 1597
BstYI RGATCY 6 cut(s) 325, 639, 1687, 1757, 1939, 2563
Bsu36I CCTNAGG 1 cut(s) 800
BsuI GTATCC 2 cut(s) 2537, 2827
BsuRI GGCC 8 cut(s) 171, 437, 465, 762, 1701, 2208, 3358, 3568
BtgI CCRYGG 3 cut(s) 282, 643, 1590
BtgZI GCGATG 3 cut(s) 43, 159, 2963
BtsIMutI CAGTG 2 cut(s) 2795, 3501
BveI ACCTGC 3 cut(s) 696, 3358, 3523
Cac8I GCNNGC 5 cut(s) 344, 650, 1373, 2248, 3216
CaiI CAGNNNCTG 4 cut(s) 720, 2688, 3029, 3515
CciI TCATGA 1 cut(s) 1972
CfoI GCGC 1 cut(s) 342
Cfr13I GGNCC 6 cut(s) 170, 947, 2415, 2685, 3356, 3566
CpoI CGGWCCG 1 cut(s) 947
CseI GACGC 3 cut(s) 289, 306, 1646
Csp6I GTAC 9 cut(s) 369, 517, 1209, 1324, 1747, 3089, 3300, 3386, 3500
CspI CGGWCCG 1 cut(s) 947
CviQI GTAC 9 cut(s) 369, 517, 1209, 1324, 1747, 3089, 3300, 3386, 3500
DraI TTTAAA 3 cut(s) 1068, 1395, 2695
DraIII CACNNNGTG 1 cut(s) 1552
EaeI YGGCCR 2 cut(s) 435, 463
Eam1104I CTCTTC 3 cut(s) 544, 1497, 2073
EarI CTCTTC 3 cut(s) 544, 1497, 2073
Ecl136II GAGCTC 1 cut(s) 493
Eco130I CCWWGG 3 cut(s) 282, 643, 1590
Eco147I AGGCCT 1 cut(s) 1701
Eco24I GRGCYC 1 cut(s) 495
Eco31I GGTCTC 2 cut(s) 21, 1536
Eco47I GGWCC 3 cut(s) 947, 2415, 2685
Eco53kI GAGCTC 1 cut(s) 493
Eco57I CTGAAG 6 cut(s) 40, 1244, 1521, 1577, 2684, 3194
Eco81I CCTNAGG 1 cut(s) 800
Eco88I CYCGRG 2 cut(s) 678, 2033
EcoICRI GAGCTC 1 cut(s) 493
EcoO109I RGGNCCY 1 cut(s) 2685
EcoRI GAATTC 1 cut(s) 2985
EcoRII CCWGG 8 cut(s) 147, 245, 1381, 1507, 1980, 2974, 3243, 3358
EcoT14I CCWWGG 3 cut(s) 282, 643, 1590
EcoT22I ATGCAT 1 cut(s) 1452
EcoT38I GRGCYC 1 cut(s) 495
ErhI CCWWGG 3 cut(s) 282, 643, 1590
FalI AAGNNNNNCTT 2 cut(s) 1070, 1102
FaqI GGGAC 6 cut(s) 309, 950, 1011, 1480, 1998, 3265
FauI CCCGC 3 cut(s) 80, 584, 645
FblI GTMKAC 2 cut(s) 1489, 1513
Fnu4HI GCNGC 2 cut(s) 721, 2537
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 2 cut(s) 721, 2537
FspBI CTAG 8 cut(s) 521, 1280, 1361, 1376, 2427, 2943, 3150, 3413
FspI TGCGCA 1 cut(s) 341
GlaI GCGC 1 cut(s) 341
GluI GCNGC 2 cut(s) 721, 2537
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 8 cut(s) 171, 437, 465, 762, 1701, 2208, 3358, 3568
HapII CCGG 1 cut(s) 950
HgaI GACGC 3 cut(s) 289, 306, 1646
HhaI GCGC 1 cut(s) 342
Hin1I GRCGYC 2 cut(s) 5, 1638
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 4 cut(s) 9, 550, 814, 1490
HindII GTYRAC 4 cut(s) 9, 550, 814, 1490
HindIII AAGCTT 4 cut(s) 52, 1084, 2288, 2666
HpaII CCGG 1 cut(s) 950
HphI GGTGA 5 cut(s) 1055, 2603, 3251, 3382, 3457
Hpy166II GTNNAC 9 cut(s) 9, 104, 414, 550, 611, 814, 1490, 1514, 3034
Hpy8I GTNNAC 9 cut(s) 9, 104, 414, 550, 611, 814, 1490, 1514, 3034
Hpy99I CGWCG 2 cut(s) 1491, 3009
HpyAV CCTTC 8 cut(s) 339, 463, 1327, 1691, 1940, 2261, 2433, 3367
HpyCH4III ACNGT 6 cut(s) 373, 699, 1443, 1494, 2882, 3031
HpyCH4IV ACGT 3 cut(s) 607, 2595, 3388
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 654, 1456, 2205, 2256, 2612, 3364, 3409, 3515
HpySE526I ACGT 3 cut(s) 607, 2595, 3388
Hsp92I GRCGYC 2 cut(s) 5, 1638
HspAI GCGC 1 cut(s) 340
KpnI GGTACC 1 cut(s) 1750
LguI GCTCTTC 1 cut(s) 1497
LmnI GCTCC 3 cut(s) 369, 836, 1838
Lsp1109I GCAGC 1 cut(s) 707
LweI GCATC 6 cut(s) 409, 1000, 1437, 2186, 2688, 3209
MaeI CTAG 8 cut(s) 521, 1280, 1361, 1376, 2427, 2943, 3150, 3413
MaeII ACGT 3 cut(s) 607, 2595, 3388
MaeIII GTNAC 6 cut(s) 499, 967, 1348, 2576, 2591, 3025
MfeI CAATTG 2 cut(s) 2190, 2860
MflI RGATCY 6 cut(s) 325, 639, 1687, 1757, 1939, 2563
MhlI GDGCHC 3 cut(s) 106, 495, 3102
MlsI TGGCCA 2 cut(s) 437, 465
MluI ACGCGT 1 cut(s) 298
MluNI TGGCCA 2 cut(s) 437, 465
MlyI GAGTC 6 cut(s) 191, 1722, 2038, 2047, 2285, 3260
Mox20I TGGCCA 2 cut(s) 437, 465
Mph1103I ATGCAT 1 cut(s) 1452
MroXI GAANNNNTTC 6 cut(s) 571, 1050, 1085, 1482, 2067, 2958
MscI TGGCCA 2 cut(s) 437, 465
MslI CAYNNNNRTG 5 cut(s) 247, 459, 566, 1595, 3043
Msp20I TGGCCA 2 cut(s) 437, 465
MspCI CTTAAG 1 cut(s) 1753
MspI CCGG 1 cut(s) 950
MspR9I CCNGG 9 cut(s) 149, 247, 951, 1383, 1509, 1982, 2976, 3245, 3360
MunI CAATTG 2 cut(s) 2190, 2860
Mva1269I GAATGC 2 cut(s) 723, 2384
MvaI CCWGG 8 cut(s) 149, 247, 1383, 1509, 1982, 2976, 3245, 3360
MvnI CGCG 2 cut(s) 75, 300
MwoI GCNNNNNNNGC 9 cut(s) 110, 654, 1456, 2205, 2256, 2612, 3364, 3409, 3515
NciI CCSGG 1 cut(s) 951
NcoI CCATGG 3 cut(s) 282, 643, 1590
NlaIV GGNNCC 3 cut(s) 327, 641, 1748
NmeAIII GCCGAG 1 cut(s) 4
NmuCI GTSAC 2 cut(s) 1348, 2591
NsbI TGCGCA 1 cut(s) 341
NsiI ATGCAT 1 cut(s) 1452
NspI RCATGY 1 cut(s) 652
OliI CACNNNNGTG 1 cut(s) 3043
PaeI GCATGC 1 cut(s) 652
PaeR7I CTCGAG 1 cut(s) 2033
PagI TCATGA 1 cut(s) 1972
PaqCI CACCTGC 1 cut(s) 3358
PasI CCCWGGG 1 cut(s) 1981
PceI AGGCCT 1 cut(s) 1701
PciSI GCTCTTC 1 cut(s) 1497
PctI GAATGC 2 cut(s) 723, 2384
PdmI GAANNNNTTC 6 cut(s) 571, 1050, 1085, 1482, 2067, 2958
Pfl23II CGTACG 1 cut(s) 368
PflMI CCANNNNNTGG 2 cut(s) 617, 1591
PfoI TCCNGGA 1 cut(s) 2974
PkrI GCNGC 2 cut(s) 722, 2538
PleI GAGTC 6 cut(s) 191, 1722, 2037, 2046, 2284, 3259
PpsI GAGTC 6 cut(s) 191, 1722, 2037, 2046, 2284, 3259
PpuMI RGGWCCY 1 cut(s) 2685
PshBI ATTAAT 1 cut(s) 2709
PsiI TTATAA 2 cut(s) 125, 1127
Psp124BI GAGCTC 1 cut(s) 495
Psp5II RGGWCCY 1 cut(s) 2685
Psp6I CCWGG 8 cut(s) 147, 245, 1381, 1507, 1980, 2974, 3243, 3358
PspGI CCWGG 8 cut(s) 147, 245, 1381, 1507, 1980, 2974, 3243, 3358
PspLI CGTACG 1 cut(s) 368
PspN4I GGNNCC 3 cut(s) 327, 641, 1748
PspPI GGNCC 6 cut(s) 170, 947, 2415, 2685, 3356, 3566
PspPPI RGGWCCY 1 cut(s) 2685
PstI CTGCAG 3 cut(s) 2237, 2894, 3369
PstNI CAGNNNCTG 4 cut(s) 720, 2688, 3029, 3515
PsuI RGATCY 6 cut(s) 325, 639, 1687, 1757, 1939, 2563
RsaI GTAC 9 cut(s) 370, 518, 1210, 1325, 1748, 3090, 3301, 3387, 3501
RsaNI GTAC 9 cut(s) 369, 517, 1209, 1324, 1747, 3089, 3300, 3386, 3500
RseI CAYNNNNRTG 5 cut(s) 247, 459, 566, 1595, 3043
Rsr2I CGGWCCG 1 cut(s) 947
RsrII CGGWCCG 1 cut(s) 947
SacI GAGCTC 1 cut(s) 495
SalI GTCGAC 1 cut(s) 1488
SapI GCTCTTC 1 cut(s) 1497
SatI GCNGC 2 cut(s) 721, 2537
Sau96I GGNCC 6 cut(s) 170, 947, 2415, 2685, 3356, 3566
SchI GAGTC 6 cut(s) 191, 1722, 2038, 2047, 2285, 3260
ScrFI CCNGG 9 cut(s) 149, 247, 951, 1383, 1509, 1982, 2976, 3245, 3360
SduI GDGCHC 3 cut(s) 106, 495, 3102
SfaNI GCATC 6 cut(s) 409, 1000, 1437, 2186, 2688, 3209
SfcI CTRYAG 5 cut(s) 2233, 2496, 2890, 3365, 3532
Sfr274I CTCGAG 1 cut(s) 2033
SinI GGWCC 3 cut(s) 947, 2415, 2685
SlaI CTCGAG 1 cut(s) 2033
SmiMI CAYNNNNRTG 5 cut(s) 247, 459, 566, 1595, 3043
SmlI CTYRAG 7 cut(s) 199, 1403, 1723, 1753, 1928, 2033, 3332
SmoI CTYRAG 7 cut(s) 199, 1403, 1723, 1753, 1928, 2033, 3332
SphI GCATGC 1 cut(s) 652
SseBI AGGCCT 1 cut(s) 1701
SsiI CCGC 4 cut(s) 73, 577, 652, 2537
SspMI CTAG 8 cut(s) 521, 1280, 1361, 1376, 2427, 2943, 3150, 3413
SstI GAGCTC 1 cut(s) 495
StuI AGGCCT 1 cut(s) 1701
StyD4I CCNGG 9 cut(s) 147, 245, 949, 1381, 1507, 1980, 2974, 3243, 3358
StyI CCWWGG 3 cut(s) 282, 643, 1590
TaaI ACNGT 6 cut(s) 373, 699, 1443, 1494, 2882, 3031
TaiI ACGT 3 cut(s) 610, 2598, 3391
TaqI TCGA 9 cut(s) 277, 458, 1445, 1489, 2034, 2157, 3007, 3481, 3524
TaqII GACCGA 1 cut(s) 935
TatI WGTACW 3 cut(s) 1208, 1323, 3088
TauI GCSGC 1 cut(s) 2539
TscAI CASTG 2 cut(s) 2802, 3501
TseFI GTSAC 2 cut(s) 1348, 2591
TseI GCWGC 1 cut(s) 720
Tsp45I GTSAC 2 cut(s) 1348, 2591
TspGWI ACGGA 5 cut(s) 356, 492, 518, 1137, 2576
TspRI CASTG 2 cut(s) 2802, 3501
Van91I CCANNNNNTGG 2 cut(s) 617, 1591
Vha464I CTTAAG 1 cut(s) 1753
VneI GTGCAC 1 cut(s) 102
VpaK11BI GGWCC 3 cut(s) 947, 2415, 2685
VspI ATTAAT 1 cut(s) 2709
XapI RAATTY 7 cut(s) 229, 524, 1648, 1771, 1846, 2937, 2985
XbaI TCTAGA 1 cut(s) 2426
XceI RCATGY 1 cut(s) 652
XhoI CTCGAG 1 cut(s) 2033
XmiI GTMKAC 2 cut(s) 1489, 1513
XmnI GAANNNNTTC 6 cut(s) 571, 1050, 1085, 1482, 2067, 2958
XspI CTAG 8 cut(s) 521, 1280, 1361, 1376, 2427, 2943, 3150, 3413
Zsp2I ATGCAT 1 cut(s) 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.