RchiOBHm_Chr6g0250621

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000105
Physical Location & Seq
Forward (+)
6039036 .. 6044232
5197 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22466

Sequence Viewer

Length: 3552 bp
ATGACGTCAGCTTCGTCTACTCGTCGACGATGGAAATACGATGTGTTTCTAAGCTTCAGAGGGGAAGATACCCGCGAGACTTTCATCTGCCATCTGTATCGTGCACTAACTGCGAGAGGAATTATAACCTTCATAGATGACGAAAGCCTGGAGAGAGGATCAACCATTGGCCCATCACTTTTTACAGCAATTGAGAACTCAAGAATTGCCCTTGTCGTTCTTTCAGAGAATTTCGCTTCTTCATCGTGGTGCGTGGATGAACTCTTGAAGATTCTTCAATGCTGGGATGAGAGGAGACTAATAGCCTACCCGATCTTCTACGGAGTGGATCCTTCTGATGTGCGCAAGCAAAGGGAAAGTTTTCAGCTCCGTACAGTAGAGGTAAGGGAACATGAAGAAGTTTATGGGAATAAGGACAAACTAAAGCAGTGGATATATGCTTTGAAAACAGTGGCCAATCTTGCTGGGTGGGTTTCGAATGATTATAAGGACAACGGAAAACTTATTGAGGCAATTGTTAGGTATACACTAGGAAAACTAGGTGATACTTATACCTCTTCAACTGTTGGCAAGGGCCTCATAGGAATGGATTCCCGCATTGAGGATTTATTAAGCAAATACATATGTCCTCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCTAGCCATCCGTGATCGAATTTGTCAGGATTTTGACCAAAGCTGCTTTCTTTCCAATGTTAGAGAAAAATCTCTAGTTTCTCTACAAGAAAAACTTGTTTCTAAAATCCTGAGGACAAAGATTGAAAACATAGATGATGAACACGCAGGAGCTGCTATGATAAAAAGGCGGTCATGTCTACTAAAGGTGCTTGTTGTTATTGATGATGTGGATCACATTAATCAATTAGATAAATTGGCTGGAAGTCGGGACTGGTTTGGTCCTGGGAGTAGAATTATCATAACCACCCCAGATATTCATTTGCTAAGGGATGTTGATGATACATACAAGGCTACTGGATTAACTCGTGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAACAGCTTCCCACCAGAAGATTATTCGGACTTGTGCCACCATATTGTAGGGTATGCTCAAGGACTTCCGTTGGCTCTTGTGGTTTTAGGTTCTTTTCTATCTGGTAGAAGCACCAATGAATGGGAAAGTACAATAGAGAGGCTACACAATACACCAAATAGACAAGTTATGGATGTGCTTCAGATTAGTTTTGACGGACTAGAGGAAAAAGACCAACAGATATTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGAACCTGTCATTGGTTTAAAGGTTCTTGAGGAAAAATCTCTCATTACTATTTTTAAGAACAAACTGTCTATGCATGATTTGCTACAAGAAATGGGCTTCGAAATAGTTCGTCGAGAGTCCCCTGATGAGCCAGGTAAACGAAGTAGATTATGGTCTCCTAATGTTATCCACAATGTGCTGAAGAAAAATAAGGGAACAGACAAAATCCGAGCCATGGTAATGGACTTGCCTGAGTTAGAAGTGGCTCATTGGAAACCAGAAGCCTTTTCAAATTTGACTCAACTCAGTCTTCTCCATATTCGTAACGTGAATCTTCCCGAAGGGCTCACTTCTCTATCTAATTCCTTGAGACTCCTTGATTGGAGTGGGTACCCCTTAAGATCCCTCCCACAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCGCAGCAACATTGAACACCTCTGGAAAGGAGCAAAGAATTTTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCAGACCCCAGACCTCACAGGCGTTCAAAATCTTGAGACTCTAGATCTTGAAGGATGTAAGACTTTGGTTGAACTTCATCAATCCCTCGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAAGACTGCGAGAGTCTCGAGAGTCTGCCAAGTAAAATTGAAATGGAATCTCTTGAAACTTTAATTCTTTCTAACTGCTCAAAAGTTAAAAAGATTCCCCAGTTTGCTGGAAATATGGGGCGCTTATCAAAGCTTTATCTCGATGAGACAGCCATTGAAACACTACCTGTGTCAATTGGATGTCTGAGTGGCCTTGCTTCACTGAATCTGAGCAACTGCAAAAATCTTGTTTGCCTTCCAAGCACCATTAGTAGATTGGAGTCTCTTCAAAATCTTGATCTTTCTGGATGTTTGAAACTTGGCAAACAGGATAGTGCGAGGGAATATGGTGCAATTAGAACTGCTGTAAATCATCTTACAACGTGCTTTTGTGAAGTTGTTGGGACATATTTGTCATGGTCCTGGCCTTCTAGATTGGTGCAAAGAGCCGATATAGGACAATTGAGTATGCAGTTGCCTGTGTCTGATCTGTGTTATTCTACAGAGCAAAATGCAAATACATCAATGAGTATCTGGTTCCCACCATCTCGTCTATGTAATTTAACAGATCTAAACATAAGTAACTGCAATCTTGGTGATGCTGCGTTTGCCAACTTTGCCTGTTTTCCCTCCTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTAAGCTTCAGAACATTAATTTGGAGAATTGCAAGACACTTCAAGAGTTGTCTGGACTTCCATCAAATAGAAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAGATGCTGTTTGATGTATCCAATTTCAATAGTTTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTGCAGTAACATAGCATTTGAAATGCTACGGATATTCCTTGATCAGCAAATTTCTAGTGCGACGGAAACTTTTCAAATTGTAATTCCTGGAAATAGAATTCCTGAGTGGTTTGATCATCGAAGATTTGGGAGTTCATTAACTGCAGACCTACTTCCAGGTTGGAATAAGAGTAGGTTTTTGGGATTTGTTTTGTGTGCTGTTTTTGTACTCCATGAGCACCGTCAGGTTGATGAGCTTGATGTCCTGACGTTCAAGACTTTTAAGGCTACTCATCATCTTGTATGTTGCCTGAAGCTGAATGGAAAAGAATTAGAAGTATATGGCAAACAGCCTGCATTTCGCTTTAGCGAACAATTTTGCCAGGTTGAGTCAGATCACCTTTGGATATTCTTTGTGTCCCGTGATAAATACTTTGGTACAGATTGGTGGCATAACAGTTGCCGTCAGCTTGAGTTCTTATTTGAAACAAGAGGGCCAGGTCTGGAGGTGAAGGAGTGTGGGGTACGTCTGATATATGAGCAAGATGTGCTAGAGCTGAACCAAACAATAACTCAATCAAGCAGTAGGATGTCTTCTCCATATGAGGATATATTGAAATTATTGATTGATTTTGAGAATCGAGTTGAAGGGGAAACCAGTGGTACTGGTAGCAGAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1183

Amino Acids

134.2

Weight (kDa)

6.26

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 195 1.9e-47 TIR domain
TIR_2 PF13676 15 - 124 2e-12 TIR domain
NB-ARC PF00931 214 - 363 5.2e-14 NB-ARC domain
WHD_ROQ1 PF23282 429 - 500 7.3e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 592 - 718 1.8e-06 Leucine-rich repeat region
LRR_13 PF23286 689 - 785 2.1e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 700 - 890 6e-08 Leucine-rich repeat region
LRR_13 PF23286 855 - 941 9e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 992 - 1140 8.8e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 125, 486
AasI GACNNNNNNGTC 1 cut(s) 2410
AatII GACGTC 1 cut(s) 8
Acc16I TGCGCA 1 cut(s) 344
Acc65I GGTACC 1 cut(s) 1749
AccB1I GGYRCC 1 cut(s) 1749
AccB7I CCANNNNNTGG 1 cut(s) 1203
AccI GTMKAC 4 cut(s) 17, 25, 524, 874
AccII CGCG 1 cut(s) 75
AciI CCGC 5 cut(s) 73, 595, 670, 865, 1813
AclWI GGATC 8 cut(s) 166, 323, 336, 652, 665, 915, 1353, 1755
AcoI YGGCCR 1 cut(s) 453
AcsI RAATTY 7 cut(s) 229, 714, 1651, 1774, 1849, 2940, 2988
AcuI CTGAAG 5 cut(s) 40, 1247, 1580, 2687, 3203
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1555
AfaI GTAC 8 cut(s) 373, 1213, 1328, 1751, 3099, 3310, 3396, 3535
AfiI CCNNNNNNNGG 5 cut(s) 601, 635, 1203, 1391, 1594
AflII CTTAAG 1 cut(s) 1756
AjnI CCWGG 8 cut(s) 147, 958, 1510, 2420, 2977, 3046, 3252, 3367
AjuI GAANNNNNNNTTGG 6 cut(s) 726, 758, 1374, 1406, 2699, 2731
Alw21I GWGCWC 2 cut(s) 106, 3111
Alw26I GTCTC 8 cut(s) 71, 289, 1539, 1723, 1928, 2039, 2159, 2286
Alw44I GTGCAC 1 cut(s) 102
AlwI GGATC 8 cut(s) 166, 323, 336, 652, 665, 915, 1353, 1755
AlwNI CAGNNNCTG 3 cut(s) 848, 2691, 3549
Ama87I CYCGRG 1 cut(s) 2036
AoxI GGCC 6 cut(s) 169, 453, 574, 2209, 2423, 3365
ApaLI GTGCAC 1 cut(s) 102
ApeKI GCWGC 5 cut(s) 738, 848, 1815, 2600, 2892
ApoI RAATTY 7 cut(s) 229, 714, 1651, 1774, 1849, 2940, 2988
ArsI GACNNNNNNTTYG 1 cut(s) 27
AseI ATTAAT 2 cut(s) 915, 2712
Asp700I GAANNNNTTC 6 cut(s) 360, 589, 1053, 1088, 1485, 2961
Asp718I GGTACC 1 cut(s) 1749
AspLEI GCGC 2 cut(s) 345, 2142
AspS9I GGNCC 6 cut(s) 170, 574, 956, 2418, 2688, 3365
AsuHPI GGTGA 5 cut(s) 554, 1058, 2606, 3260, 3391
AsuII TTCGAA 2 cut(s) 476, 1479
AvaI CYCGRG 1 cut(s) 2036
AvaII GGWCC 3 cut(s) 956, 2418, 2688
AxyI CCTNAGG 1 cut(s) 806
BaeGI GKGCMC 1 cut(s) 106
BalI TGGCCA 1 cut(s) 455
BamHI GGATCC 2 cut(s) 328, 657
BanI GGYRCC 1 cut(s) 1749
BanII GRGCYC 1 cut(s) 1707
BarI GAAGNNNNNNTAC 2 cut(s) 3448, 3480
BauI CACGAG 1 cut(s) 1041
BbsI GAAGAC 2 cut(s) 1661, 3456
Bbv12I GWGCWC 2 cut(s) 106, 3111
BbvCI CCTCAGC 1 cut(s) 630
BbvI GCAGC 5 cut(s) 725, 835, 1827, 2587, 2879
BccI CCATC 7 cut(s) 24, 99, 181, 710, 2551, 2764, 2780
BceAI ACGGC 1 cut(s) 3318
BciT130I CCWGG 8 cut(s) 149, 960, 1512, 2422, 2979, 3048, 3254, 3369
BciVI GTATCC 1 cut(s) 2830
BclI TGATCA 2 cut(s) 2932, 3004
BcoDI GTCTC 8 cut(s) 71, 289, 1539, 1723, 1928, 2039, 2159, 2286
BfmI CTRYAG 3 cut(s) 2499, 2893, 3033
BfoI RGCGCY 1 cut(s) 2143
BfrI CTTAAG 1 cut(s) 1756
BfuI GTATCC 1 cut(s) 2830
BglII AGATCT 2 cut(s) 1942, 2566
BisI GCNGC 6 cut(s) 739, 849, 1813, 1816, 2601, 2893
BlsI GCNGC 6 cut(s) 740, 850, 1814, 1817, 2602, 2894
Bme1390I CCNGG 8 cut(s) 149, 960, 1512, 2422, 2979, 3048, 3254, 3369
Bme18I GGWCC 3 cut(s) 956, 2418, 2688
BmeT110I CYCGRG 1 cut(s) 2036
BmgT120I GGNCC 6 cut(s) 170, 574, 956, 2418, 2688, 3365
BmiI GGNNCC 4 cut(s) 330, 659, 1751, 2537
BmrFI CCNGG 8 cut(s) 149, 960, 1512, 2422, 2979, 3048, 3254, 3369
BmrI ACTGGG 1 cut(s) 2113
BmsI GCATC 3 cut(s) 2587, 2691, 2796
BmuI ACTGGG 1 cut(s) 2113
BpiI GAAGAC 2 cut(s) 1661, 3456
BpmI CTGGAG 3 cut(s) 170, 2822, 3395
Bpu10I CCTNAGC 2 cut(s) 630, 1001
Bpu14I TTCGAA 2 cut(s) 476, 1479
BpuEI CTTGAG 6 cut(s) 184, 1125, 1427, 1747, 1952, 3362
BsaBI GATNNNNATC 1 cut(s) 906
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1539
BsaJI CCNNGG 4 cut(s) 661, 959, 1593, 1984
BsaXI ACNNNNNCTCC 2 cut(s) 2795, 2825
Bsc4I CCNNNNNNNGG 5 cut(s) 601, 635, 1203, 1391, 1594
Bse1I ACTGG 6 cut(s) 953, 1036, 2119, 2805, 3528, 3541
Bse21I CCTNAGG 1 cut(s) 806
Bse8I GATNNNNATC 1 cut(s) 906
BseBI CCWGG 8 cut(s) 149, 960, 1512, 2422, 2979, 3048, 3254, 3369
BseDI CCNNGG 4 cut(s) 661, 959, 1593, 1984
BseJI GATNNNNATC 1 cut(s) 906
BseLI CCNNNNNNNGG 5 cut(s) 601, 635, 1203, 1391, 1594
BseMII CTCAG 8 cut(s) 644, 797, 1602, 1678, 2195, 2219, 2639, 2985
BseNI ACTGG 6 cut(s) 953, 1036, 2119, 2805, 3528, 3541
BseRI GAGGAG 1 cut(s) 307
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 5 cut(s) 725, 835, 1827, 2587, 2879
BseYI CCCAGC 2 cut(s) 282, 464
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 6 cut(s) 171, 455, 576, 2211, 2425, 3367
BshNI GGYRCC 1 cut(s) 1749
BsiHKAI GWGCWC 2 cut(s) 106, 3111
BsiHKCI CYCGRG 1 cut(s) 2036
BslFI GGGAC 4 cut(s) 959, 1483, 2416, 3274
BslI CCNNNNNNNGG 5 cut(s) 601, 635, 1203, 1391, 1594
BsmAI GTCTC 8 cut(s) 71, 289, 1539, 1723, 1928, 2039, 2159, 2286
BsmFI GGGAC 4 cut(s) 959, 1483, 2416, 3274
BsnI GGCC 6 cut(s) 171, 455, 576, 2211, 2425, 3367
Bso31I GGTCTC 1 cut(s) 1539
BsoBI CYCGRG 1 cut(s) 2036
Bsp119I TTCGAA 2 cut(s) 476, 1479
Bsp1286I GDGCHC 3 cut(s) 106, 1707, 3111
Bsp1407I TGTACA 1 cut(s) 1326
Bsp19I CCATGG 2 cut(s) 661, 1593
BspACI CCGC 5 cut(s) 73, 595, 670, 865, 1813
BspANI GGCC 6 cut(s) 171, 455, 576, 2211, 2425, 3367
BspCNI CTCAG 8 cut(s) 643, 798, 1603, 1677, 2196, 2220, 2640, 2986
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 4 cut(s) 330, 659, 1751, 2537
BspMAI CTGCAG 2 cut(s) 2897, 3037
BspPI GGATC 8 cut(s) 166, 323, 336, 652, 665, 915, 1353, 1755
BspT104I TTCGAA 2 cut(s) 476, 1479
BspT107I GGYRCC 1 cut(s) 1749
BspTI CTTAAG 1 cut(s) 1756
BspTNI GGTCTC 1 cut(s) 1539
BsrGI TGTACA 1 cut(s) 1326
BsrI ACTGG 6 cut(s) 953, 1036, 2119, 2805, 3528, 3541
BssECI CCNNGG 4 cut(s) 661, 959, 1593, 1984
BssNAI GTATAC 1 cut(s) 525
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1041
BssT1I CCWWGG 2 cut(s) 661, 1593
Bst1107I GTATAC 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 1041
Bst2UI CCWGG 8 cut(s) 149, 960, 1512, 2422, 2979, 3048, 3254, 3369
Bst4CI ACNGT 6 cut(s) 376, 451, 565, 1446, 3113, 3329
Bst6I CTCTTC 2 cut(s) 562, 2289
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1756
BstAPI GCANNNNNTGC 4 cut(s) 110, 848, 1459, 3418
BstAUI TGTACA 1 cut(s) 1326
BstBI TTCGAA 2 cut(s) 476, 1479
BstC8I GCNNGC 4 cut(s) 347, 668, 1376, 3225
BstDSI CCRYGG 2 cut(s) 661, 1593
BstFNI CGCG 1 cut(s) 75
BstH2I RGCGCY 1 cut(s) 2143
BstHHI GCGC 2 cut(s) 345, 2142
BstMAI GTCTC 8 cut(s) 71, 289, 1539, 1723, 1928, 2039, 2159, 2286
BstMWI GCNNNNNNNGC 9 cut(s) 110, 461, 672, 848, 1459, 2259, 2606, 2615, 3418
BstNI CCWGG 8 cut(s) 149, 960, 1512, 2422, 2979, 3048, 3254, 3369
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 8 cut(s) 147, 958, 1510, 2420, 2977, 3046, 3252, 3367
BstSFI CTRYAG 3 cut(s) 2499, 2893, 3033
BstSLI GKGCMC 1 cut(s) 106
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 5 cut(s) 725, 835, 1827, 2587, 2879
BstV2I GAAGAC 2 cut(s) 1661, 3456
BstX2I RGATCY 5 cut(s) 328, 657, 1760, 1942, 2566
BstXI CCANNNNNNTGG 2 cut(s) 1600, 2126
BstYI RGATCY 5 cut(s) 328, 657, 1760, 1942, 2566
BstZ17I GTATAC 1 cut(s) 525
Bsu36I CCTNAGG 1 cut(s) 806
BsuI GTATCC 1 cut(s) 2830
BsuRI GGCC 6 cut(s) 171, 455, 576, 2211, 2425, 3367
BtgI CCRYGG 2 cut(s) 661, 1593
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 5 cut(s) 434, 456, 2219, 2798, 3535
Cac8I GCNNGC 4 cut(s) 347, 668, 1376, 3225
CaiI CAGNNNCTG 3 cut(s) 848, 2691, 3549
CfoI GCGC 2 cut(s) 345, 2142
Cfr13I GGNCC 6 cut(s) 170, 574, 956, 2418, 2688, 3365
Csp6I GTAC 8 cut(s) 372, 1212, 1327, 1750, 3098, 3309, 3395, 3534
CviAII CATG 8 cut(s) 392, 662, 667, 870, 1454, 1594, 2415, 3104
CviQI GTAC 8 cut(s) 372, 1212, 1327, 1750, 3098, 3309, 3395, 3534
DraI TTTAAA 2 cut(s) 1071, 1398
DraIII CACNNNGTG 1 cut(s) 1555
DrdI GACNNNNNNGTC 1 cut(s) 2410
DseDI GACNNNNNNGTC 1 cut(s) 2410
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 2 cut(s) 562, 2289
EarI CTCTTC 2 cut(s) 562, 2289
Eco130I CCWWGG 2 cut(s) 661, 1593
Eco24I GRGCYC 1 cut(s) 1707
Eco31I GGTCTC 1 cut(s) 1539
Eco47I GGWCC 3 cut(s) 956, 2418, 2688
Eco57I CTGAAG 5 cut(s) 40, 1247, 1580, 2687, 3203
Eco81I CCTNAGG 1 cut(s) 806
Eco88I CYCGRG 1 cut(s) 2036
EcoO109I RGGNCCY 2 cut(s) 574, 2688
EcoRI GAATTC 1 cut(s) 2988
EcoRII CCWGG 8 cut(s) 147, 958, 1510, 2420, 2977, 3046, 3252, 3367
EcoT14I CCWWGG 2 cut(s) 661, 1593
EcoT22I ATGCAT 1 cut(s) 1455
EcoT38I GRGCYC 1 cut(s) 1707
ErhI CCWWGG 2 cut(s) 661, 1593
FaeI CATG 8 cut(s) 395, 665, 670, 873, 1457, 1597, 2418, 3107
FalI AAGNNNNNCTT 4 cut(s) 774, 806, 1073, 1105
FaqI GGGAC 4 cut(s) 959, 1483, 2416, 3274
FatI CATG 8 cut(s) 391, 661, 666, 869, 1453, 1593, 2414, 3103
FauI CCCGC 3 cut(s) 80, 602, 663
FauNDI CATATG 2 cut(s) 623, 3472
FbaI TGATCA 2 cut(s) 2932, 3004
FblI GTMKAC 4 cut(s) 17, 25, 524, 874
Fnu4HI GCNGC 6 cut(s) 739, 849, 1813, 1816, 2601, 2893
FriOI GRGCYC 1 cut(s) 1707
Fsp4HI GCNGC 6 cut(s) 739, 849, 1813, 1816, 2601, 2893
FspI TGCGCA 1 cut(s) 344
GlaI GCGC 2 cut(s) 344, 2141
GluI GCNGC 6 cut(s) 739, 849, 1813, 1816, 2601, 2893
GsaI CCCAGC 2 cut(s) 286, 468
GsuI CTGGAG 3 cut(s) 170, 2822, 3395
HaeII RGCGCY 1 cut(s) 2143
HaeIII GGCC 6 cut(s) 171, 455, 576, 2211, 2425, 3367
HhaI GCGC 2 cut(s) 345, 2142
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 395, 665, 670, 873, 1457, 1597, 2418, 3107
Hin6I GCGC 2 cut(s) 343, 2140
HinP1I GCGC 2 cut(s) 343, 2140
HincII GTYRAC 1 cut(s) 26
HindII GTYRAC 1 cut(s) 26
HindIII AAGCTT 4 cut(s) 52, 2150, 2669, 2699
HphI GGTGA 5 cut(s) 554, 1058, 2606, 3260, 3391
Hpy166II GTNNAC 6 cut(s) 18, 26, 104, 525, 875, 1517
Hpy8I GTNNAC 6 cut(s) 18, 26, 104, 525, 875, 1517
Hpy99I CGWCG 4 cut(s) 27, 30, 1494, 2956
HpyAV CCTTC 9 cut(s) 139, 342, 1330, 1694, 1943, 2264, 2436, 3376, 3512
HpyCH4III ACNGT 6 cut(s) 376, 451, 565, 1446, 3113, 3329
HpyCH4IV ACGT 5 cut(s) 5, 1686, 2381, 3140, 3397
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 461, 672, 848, 1459, 2259, 2606, 2615, 3418
HpySE526I ACGT 5 cut(s) 5, 1686, 2381, 3140, 3397
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 395, 665, 670, 873, 1457, 1597, 2418, 3107
HspAI GCGC 2 cut(s) 343, 2140
KpnI GGTACC 1 cut(s) 1753
Ksp22I TGATCA 2 cut(s) 2932, 3004
LmnI GCTCC 3 cut(s) 372, 845, 1841
Lsp1109I GCAGC 5 cut(s) 725, 835, 1827, 2587, 2879
LweI GCATC 3 cut(s) 2587, 2691, 2796
MaeII ACGT 5 cut(s) 5, 1686, 2381, 3140, 3397
MaeIII GTNAC 4 cut(s) 1351, 1682, 2579, 2897
MfeI CAATTG 5 cut(s) 189, 513, 2193, 2459, 2863
MflI RGATCY 5 cut(s) 328, 657, 1760, 1942, 2566
MhlI GDGCHC 3 cut(s) 106, 1707, 3111
MlsI TGGCCA 1 cut(s) 455
MluNI TGGCCA 1 cut(s) 455
MlyI GAGTC 8 cut(s) 1505, 1651, 1725, 1930, 2041, 2050, 2288, 3269
MmeI TCCRAC 1 cut(s) 3032
Mox20I TGGCCA 1 cut(s) 455
Mph1103I ATGCAT 1 cut(s) 1455
MroXI GAANNNNTTC 6 cut(s) 360, 589, 1053, 1088, 1485, 2961
MscI TGGCCA 1 cut(s) 455
MslI CAYNNNNRTG 3 cut(s) 247, 584, 1598
Msp20I TGGCCA 1 cut(s) 455
MspCI CTTAAG 1 cut(s) 1756
MspR9I CCNGG 8 cut(s) 149, 960, 1512, 2422, 2979, 3048, 3254, 3369
MunI CAATTG 5 cut(s) 189, 513, 2193, 2459, 2863
MvaI CCWGG 8 cut(s) 149, 960, 1512, 2422, 2979, 3048, 3254, 3369
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 9 cut(s) 110, 461, 672, 848, 1459, 2259, 2606, 2615, 3418
NcoI CCATGG 2 cut(s) 661, 1593
NdeI CATATG 2 cut(s) 623, 3472
NlaIII CATG 8 cut(s) 395, 665, 670, 873, 1457, 1597, 2418, 3107
NlaIV GGNNCC 4 cut(s) 330, 659, 1751, 2537
NmuCI GTSAC 1 cut(s) 1351
NsbI TGCGCA 1 cut(s) 344
NsiI ATGCAT 1 cut(s) 1455
NspI RCATGY 1 cut(s) 670
NspV TTCGAA 2 cut(s) 476, 1479
PaeI GCATGC 1 cut(s) 670
PaeR7I CTCGAG 1 cut(s) 2036
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdmI GAANNNNTTC 6 cut(s) 360, 589, 1053, 1088, 1485, 2961
PfeI GAWTC 8 cut(s) 271, 590, 1690, 2014, 2066, 2113, 2224, 3508
PflMI CCANNNNNTGG 1 cut(s) 1203
PfoI TCCNGGA 1 cut(s) 2977
PkrI GCNGC 6 cut(s) 740, 850, 1814, 1817, 2602, 2894
PleI GAGTC 8 cut(s) 1504, 1651, 1725, 1930, 2040, 2049, 2287, 3268
PpsI GAGTC 8 cut(s) 1504, 1651, 1725, 1930, 2040, 2049, 2287, 3268
PpuMI RGGWCCY 1 cut(s) 2688
PshBI ATTAAT 2 cut(s) 915, 2712
PsiI TTATAA 2 cut(s) 125, 486
Psp5II RGGWCCY 1 cut(s) 2688
Psp6I CCWGG 8 cut(s) 147, 958, 1510, 2420, 2977, 3046, 3252, 3367
PspFI CCCAGC 2 cut(s) 282, 464
PspGI CCWGG 8 cut(s) 147, 958, 1510, 2420, 2977, 3046, 3252, 3367
PspN4I GGNNCC 4 cut(s) 330, 659, 1751, 2537
PspPI GGNCC 6 cut(s) 170, 574, 956, 2418, 2688, 3365
PspPPI RGGWCCY 1 cut(s) 2688
PstI CTGCAG 2 cut(s) 2897, 3037
PstNI CAGNNNCTG 3 cut(s) 848, 2691, 3549
PsuI RGATCY 5 cut(s) 328, 657, 1760, 1942, 2566
RsaI GTAC 8 cut(s) 373, 1213, 1328, 1751, 3099, 3310, 3396, 3535
RsaNI GTAC 8 cut(s) 372, 1212, 1327, 1750, 3098, 3309, 3395, 3534
RseI CAYNNNNRTG 3 cut(s) 247, 584, 1598
SalI GTCGAC 1 cut(s) 24
SatI GCNGC 6 cut(s) 739, 849, 1813, 1816, 2601, 2893
Sau96I GGNCC 6 cut(s) 170, 574, 956, 2418, 2688, 3365
SchI GAGTC 8 cut(s) 1505, 1651, 1725, 1930, 2041, 2050, 2288, 3269
ScrFI CCNGG 8 cut(s) 149, 960, 1512, 2422, 2979, 3048, 3254, 3369
SduI GDGCHC 3 cut(s) 106, 1707, 3111
SfaNI GCATC 3 cut(s) 2587, 2691, 2796
SfcI CTRYAG 3 cut(s) 2499, 2893, 3033
Sfr274I CTCGAG 1 cut(s) 2036
SfuI TTCGAA 2 cut(s) 476, 1479
SgrDI CGTCGACG 1 cut(s) 24
SinI GGWCC 3 cut(s) 956, 2418, 2688
SlaI CTCGAG 1 cut(s) 2036
SmiMI CAYNNNNRTG 3 cut(s) 247, 584, 1598
SmlI CTYRAG 8 cut(s) 199, 1140, 1406, 1726, 1756, 1931, 2036, 3341
SmoI CTYRAG 8 cut(s) 199, 1140, 1406, 1726, 1756, 1931, 2036, 3341
SphI GCATGC 1 cut(s) 670
SsiI CCGC 5 cut(s) 73, 595, 670, 865, 1813
StyD4I CCNGG 8 cut(s) 147, 958, 1510, 2420, 2977, 3046, 3252, 3367
StyI CCWWGG 2 cut(s) 661, 1593
TaaI ACNGT 6 cut(s) 376, 451, 565, 1446, 3113, 3329
TaiI ACGT 5 cut(s) 8, 1689, 2384, 3143, 3400
TaqI TCGA 9 cut(s) 25, 476, 712, 1479, 1492, 2037, 2160, 3010, 3511
TatI WGTACW 3 cut(s) 1211, 1326, 3097
TauI GCSGC 1 cut(s) 1815
TfiI GAWTC 8 cut(s) 271, 590, 1690, 2014, 2066, 2113, 2224, 3508
TscAI CASTG 5 cut(s) 434, 456, 2226, 2805, 3535
TseFI GTSAC 1 cut(s) 1351
TseI GCWGC 5 cut(s) 738, 848, 1815, 2600, 2892
Tsp45I GTSAC 1 cut(s) 1351
TspGWI ACGGA 8 cut(s) 336, 359, 510, 695, 1140, 1293, 2935, 2969
TspRI CASTG 5 cut(s) 434, 456, 2226, 2805, 3535
Van91I CCANNNNNTGG 1 cut(s) 1203
Vha464I CTTAAG 1 cut(s) 1756
VneI GTGCAC 1 cut(s) 102
VpaK11BI GGWCC 3 cut(s) 956, 2418, 2688
VspI ATTAAT 2 cut(s) 915, 2712
XapI RAATTY 7 cut(s) 229, 714, 1651, 1774, 1849, 2940, 2988
XbaI TCTAGA 2 cut(s) 1939, 2429
XceI RCATGY 1 cut(s) 670
XcmI CCANNNNNNNNNTGG 1 cut(s) 2272
XhoI CTCGAG 1 cut(s) 2036
XmiI GTMKAC 4 cut(s) 17, 25, 524, 874
XmnI GAANNNNTTC 6 cut(s) 360, 589, 1053, 1088, 1485, 2961
ZraI GACGTC 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.