Rw1G005020

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
9598229 .. 9602469
4241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005020.1

Sequence Viewer

Length: 3600 bp
ATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGGCCCGAACTTTTAGCTGCAATTGAGGACTCTAGATCTGCGATTGTCATTCTCTCATGGAGATATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAAACATGGGCCAACAAGTCTTCCCCGTCTTCTACTTGGTGGATCCTTCTGATGTGCGGCACCAGACGGGAAGTTTTGAGCTCAAAAGGAAAACCCAAGTAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGGACAGAATGAGGACAGACTTTCTGGATGGAGAGCTGCTTTGAGAGAGGTGGCCAATCTTTCTGGATGGCATGCCCGTGCTCCTGCTTTGACACAAGTCGCTAAGAATTTCAAGAATAATCCAAATGACCCAATACTAATTGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATGCATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGAATTCCCGCATTCATGATTTATTAAGCAATTACATATGTCCACAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATTTGTCAGGATTTTGACCGATCCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAATTTGAAAATATAGAGGATGAATACACAGGAGCTACTATGATACAAAGGCGGTTGTGTAGAAAAAAGGTGCTTGTTGTTATTGATGATGTGGATCTGTTGACACAATTAGAGAAATTGGCTGGAAGTCCCAACTGGTTTGGTCCTGGGAGTAGAATTATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGGTGCTACATACAAGGCTAACGGGTTAAACTATGAGGAAGCACTCCAACTTTTGAGTTTGAAGGCTTTTAAGAAATATCCCCCACCAGAAGATTATTTGCATCTGTGCAACTGTATTTTAGAGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATCTGGTAGAAACACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTCACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAACCATCTCCAGCAACGAACTGTCGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTGTGGTCTCATGAAGACATCAACAATGTGCTGAACGAAAATAAGGCAGATTTACTTGTAATCTGCTTCGGTCTTGGTATTTGTTGTGTTCTGCACTTAAGCTATTATTCTTGCATCGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAAGCCTGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCCGAAGGCCTCACTTGTTTTTCTAATTCTTTGAGACTCCTCGAATGGACTGGGTGTCCCTTAAGATCTCTCCCAAAAAACTTTAAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTCTGGAAGGGAACAACGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAAAATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCAAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAATATTAATTCTTTCTAACTGCTCAAAAGTTCAGAAGATTCCCGAGTTTGTAGGAAATATGGAACGTTTGTCGGTGCTTTATCTTGATGAGACTACCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGACATTGAATCTAAGCAACTGCAGAAATCTTGTTTGTCTTCCAAGGACCATCAATAAATTGAAGTCTCTTGAAAATCTTAATCTTTCTGGATGCTTGAATGTGGGGCAGATGGACTGTTTTGAGGAAACTAACGTGAATAGTGGGTGCATATCATCTGCCCTTGATTGCATAAAATATGTGATAGGTTCAATCTTTCCTGGATGCAAAGTAGTTTGGGGATTTTTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAACTGAATACAGAGCCAATGAGTTTCCGCTTGCCTATATCTCAAAAAGTGAATACAGAGCCAACAAGTTTCATCTTGCCTATATCTCAAAAAGTGAATATAGAGCCAACAAGTTTCCGCTTGCCTATTTCTGGTCTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTCGCCAACGAATTCGGTTACTTTCCCTCCTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTACGCATTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAATTTGGAGAATTGCAAGAGACTCCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTCTATTTAAGGGCAGATGGTTGTACATCACTGAAATACTTGTTTGATGCATCAAATTTGAAAAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGAATGCAATAACATAGCATTTGAAATGCTGAAGACATCCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATCCATGAGTGGTTCAGCCATCGGAATGTTGGGTGTTCCTTAAGTGTATCTCTACCTGCACATTGGAATAAGAGTCCGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTCCTCCATGAGCACCATCCGGTGGATGAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAGAATTGGAAGTGTATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAGTCAGATCACCTGTGGCTATTCTATGTATCTTGTGATAAATACTTTGGTATAGAGAGGAGGCTTAGCGGTTTGAATCATCTTGAGTTCATATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAAGGAGTCTGGAGTCCGTCTGATATATGAGCAAGAAGTGCAAGAGTTGAACCAAACAACCACTCAATCGAGCAGTAGGATGTCTCCTTATGAGGATATCTCGATTGGTTTTGACATTCTAGTTGCAGGGGAAACTAGTGGCACTAGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1199

Amino Acids

135.72

Weight (kDa)

6.28

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 158 9.8e-30 TIR domain
TIR_2 PF13676 2 - 66 4.5e-06 TIR domain
NB-ARC PF00931 186 - 340 2.6e-18 NB-ARC domain
AAA_22 PF13401 188 - 284 6.9e-06 AAA domain
NACHT PF05729 190 - 287 1.1e-06 NACHT domain
WHD_ROQ1 PF23282 407 - 478 2.5e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 683 - 767 1.8e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 688 - 762 4.5e-10 Leucine-rich repeat region
C-JID PF20160 1006 - 1155 1.7e-15 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1889
Acc36I ACCTGC 2 cut(s) 1889, 3094
AccB1I GGYRCC 1 cut(s) 213
AccB7I CCANNNNNTGG 2 cut(s) 551, 3160
AccI GTMKAC 1 cut(s) 2809
AciI CCGC 7 cut(s) 211, 511, 586, 793, 2482, 2572, 3369
AclI AACGTT 1 cut(s) 2122
AclWI GGATC 7 cut(s) 191, 204, 568, 581, 645, 843, 1287
AcoI YGGCCR 1 cut(s) 339
AcuI CTGAAG 5 cut(s) 1641, 2703, 2981, 3245, 3437
AfaI GTAC 2 cut(s) 2709, 2836
AfiI CCNNNNNNNGG 5 cut(s) 551, 1325, 2585, 3113, 3160
AflII CTTAAG 3 cut(s) 1558, 1738, 3070
AhdI GACNNNNNGTC 1 cut(s) 1731
AhlI ACTAGT 2 cut(s) 3557, 3566
AjnI CCWGG 5 cut(s) 886, 1444, 2395, 3019, 3294
AjuI GAANNNNNNNTTGG 2 cut(s) 2741, 2773
Alw21I GWGCWC 3 cut(s) 237, 370, 3153
AlwI GGATC 7 cut(s) 191, 204, 568, 581, 645, 843, 1287
Ama87I CYCGRG 2 cut(s) 612, 2099
AoxI GGCC 7 cut(s) 38, 163, 339, 694, 1684, 2191, 3407
ApeKI GCWGC 2 cut(s) 53, 323
AseI ATTAAT 1 cut(s) 2063
Asp700I GAANNNNTTC 4 cut(s) 505, 717, 1419, 3003
AspS9I GGNCC 6 cut(s) 39, 163, 884, 1672, 2244, 3407
AsuC2I CCSGG 1 cut(s) 3411
AsuHPI GGTGA 4 cut(s) 2645, 2689, 3302, 3433
AvaI CYCGRG 2 cut(s) 612, 2099
AvaII GGWCC 3 cut(s) 884, 1672, 2244
BalI TGGCCA 1 cut(s) 341
BamHI GGATCC 2 cut(s) 196, 573
BanI GGYRCC 1 cut(s) 213
BanII GRGCYC 1 cut(s) 237
BauI CACGAG 1 cut(s) 1094
BbsI GAAGAC 6 cut(s) 166, 175, 1482, 1643, 2228, 2969
Bbv12I GWGCWC 3 cut(s) 237, 370, 3153
BbvI GCAGC 2 cut(s) 40, 310
BceAI ACGGC 1 cut(s) 2198
BciT130I CCWGG 5 cut(s) 888, 1446, 2397, 3021, 3296
BclI TGATCA 1 cut(s) 625
BcnI CCSGG 1 cut(s) 3411
BcuI ACTAGT 2 cut(s) 3557, 3566
BfaI CTAG 6 cut(s) 69, 698, 1298, 3542, 3558, 3567
BfmI CTRYAG 1 cut(s) 2218
BfrI CTTAAG 3 cut(s) 1558, 1738, 3070
BfuAI ACCTGC 2 cut(s) 1889, 3094
BglII AGATCT 4 cut(s) 71, 1349, 1742, 1924
BisI GCNGC 3 cut(s) 54, 212, 324
BlpI GCTNAGC 1 cut(s) 3365
BlsI GCNGC 3 cut(s) 55, 213, 325
Bme1390I CCNGG 6 cut(s) 888, 1446, 2397, 3021, 3296, 3411
Bme18I GGWCC 3 cut(s) 884, 1672, 2244
BmeRI GACNNNNNGTC 1 cut(s) 1731
BmeT110I CYCGRG 2 cut(s) 612, 2099
BmgT120I GGNCC 6 cut(s) 39, 163, 884, 1672, 2244, 3407
BmiI GGNNCC 3 cut(s) 198, 215, 575
BmrFI CCNGG 6 cut(s) 888, 1446, 2397, 3021, 3296, 3411
BmrI ACTGGG 1 cut(s) 1737
BmsI GCATC 8 cut(s) 479, 1054, 1374, 1584, 2279, 2390, 2848, 2870
BmuI ACTGGG 1 cut(s) 1737
BoxI GACNNNNGTC 1 cut(s) 383
BpiI GAAGAC 6 cut(s) 166, 175, 1482, 1643, 2228, 2969
BplI GAGNNNNNCTC 2 cut(s) 3506, 3538
BpmI CTGGAG 2 cut(s) 1351, 3453
Bpu10I CCTNAGC 1 cut(s) 1074
Bpu1102I GCTNAGC 1 cut(s) 3365
BpuEI CTTGAG 5 cut(s) 38, 1934, 2003, 2765, 3404
BpuMI CCSGG 1 cut(s) 3411
BsaBI GATNNNNATC 2 cut(s) 834, 1278
BsaI GGTCTC 2 cut(s) 1473, 1877
BsaJI CCNNGG 5 cut(s) 577, 887, 1966, 2240, 2673
BsaWI WCCGGW 1 cut(s) 3157
BsaXI ACNNNNNCTCC 2 cut(s) 2654, 2684
Bsc4I CCNNNNNNNGG 5 cut(s) 551, 1325, 2585, 3113, 3160
Bse1I ACTGG 4 cut(s) 881, 1221, 1732, 2194
Bse8I GATNNNNATC 2 cut(s) 834, 1278
BseBI CCWGG 5 cut(s) 888, 1446, 2397, 3021, 3296
BseDI CCNNGG 5 cut(s) 577, 887, 1966, 2240, 2673
BseJI GATNNNNATC 2 cut(s) 834, 1278
BseLI CCNNNNNNNGG 5 cut(s) 551, 1325, 2585, 3113, 3160
BseMII CTCAG 2 cut(s) 1088, 2606
BseNI ACTGG 4 cut(s) 881, 1221, 1732, 2194
BseRI GAGGAG 2 cut(s) 1706, 3373
BseXI GCAGC 2 cut(s) 40, 310
BseYI CCCAGC 1 cut(s) 1410
BsgI GTGCAG 2 cut(s) 1538, 3072
BshFI GGCC 7 cut(s) 40, 165, 341, 696, 1686, 2193, 3409
BshNI GGYRCC 1 cut(s) 213
BsiHKAI GWGCWC 3 cut(s) 237, 370, 3153
BsiHKCI CYCGRG 2 cut(s) 612, 2099
BsiSI CCGG 2 cut(s) 3158, 3410
BslFI GGGAC 2 cut(s) 855, 1719
BslI CCNNNNNNNGG 5 cut(s) 551, 1325, 2585, 3113, 3160
BsmFI GGGAC 2 cut(s) 855, 1719
BsmI GAATGC 5 cut(s) 513, 2642, 2712, 2724, 2939
BsnI GGCC 7 cut(s) 40, 165, 341, 696, 1686, 2193, 3409
Bso31I GGTCTC 2 cut(s) 1473, 1877
BsoBI CYCGRG 2 cut(s) 612, 2099
Bsp1286I GDGCHC 3 cut(s) 237, 370, 3153
Bsp1407I TGTACA 1 cut(s) 2834
Bsp1720I GCTNAGC 1 cut(s) 3365
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 7 cut(s) 211, 511, 586, 793, 2482, 2572, 3369
BspANI GGCC 7 cut(s) 40, 165, 341, 696, 1686, 2193, 3409
BspCNI CTCAG 2 cut(s) 1087, 2607
BspHI TCATGA 2 cut(s) 517, 1471
BspLI GGNNCC 3 cut(s) 198, 215, 575
BspMAI CTGCAG 1 cut(s) 2222
BspMI ACCTGC 2 cut(s) 1889, 3094
BspPI GGATC 7 cut(s) 191, 204, 568, 581, 645, 843, 1287
BspT107I GGYRCC 1 cut(s) 213
BspTI CTTAAG 3 cut(s) 1558, 1738, 3070
BspTNI GGTCTC 2 cut(s) 1473, 1877
BsrGI TGTACA 1 cut(s) 2834
BsrI ACTGG 4 cut(s) 881, 1221, 1732, 2194
BssECI CCNNGG 5 cut(s) 577, 887, 1966, 2240, 2673
BssSI CACGAG 1 cut(s) 1094
BssT1I CCWWGG 4 cut(s) 577, 1966, 2240, 2673
Bst2BI CACGAG 1 cut(s) 1094
Bst2UI CCWGG 5 cut(s) 888, 1446, 2397, 3021, 3296
Bst4CI ACNGT 4 cut(s) 1058, 1380, 1431, 2315
Bst6I CTCTTC 1 cut(s) 3232
BstAFI CTTAAG 3 cut(s) 1558, 1738, 3070
BstAPI GCANNNNNTGC 3 cut(s) 1393, 3460, 3579
BstAUI TGTACA 1 cut(s) 2834
BstC8I GCNNGC 8 cut(s) 360, 584, 1255, 1310, 2486, 2576, 3267, 3584
BstDEI CTNAG 8 cut(s) 390, 1074, 1646, 2210, 2615, 2690, 2739, 3365
BstDSI CCRYGG 1 cut(s) 577
BstEII GGTNACC 1 cut(s) 2677
BstMWI GCNNNNNNNGC 7 cut(s) 588, 1393, 2740, 3128, 3460, 3570, 3579
BstNI CCWGG 5 cut(s) 888, 1446, 2397, 3021, 3296
BstNSI RCATGY 2 cut(s) 362, 586
BstPAI GACNNNNGTC 1 cut(s) 383
BstPI GGTNACC 1 cut(s) 2677
BstSCI CCNGG 6 cut(s) 886, 1444, 2395, 3019, 3294, 3409
BstSFI CTRYAG 1 cut(s) 2218
BstV1I GCAGC 2 cut(s) 40, 310
BstV2I GAAGAC 6 cut(s) 166, 175, 1482, 1643, 2228, 2969
BstX2I RGATCY 7 cut(s) 71, 196, 573, 835, 1349, 1742, 1924
BstYI RGATCY 7 cut(s) 71, 196, 573, 835, 1349, 1742, 1924
BsuRI GGCC 7 cut(s) 40, 165, 341, 696, 1686, 2193, 3409
BtgI CCRYGG 1 cut(s) 577
BtsIMutI CAGTG 2 cut(s) 1128, 2840
BveI ACCTGC 2 cut(s) 1889, 3094
Cac8I GCNNGC 8 cut(s) 360, 584, 1255, 1310, 2486, 2576, 3267, 3584
CciI TCATGA 2 cut(s) 517, 1471
Cfr13I GGNCC 6 cut(s) 39, 163, 884, 1672, 2244, 3407
CseI GACGC 1 cut(s) 17
Csp6I GTAC 2 cut(s) 2708, 2835
CviQI GTAC 2 cut(s) 2708, 2835
DdeI CTNAG 8 cut(s) 390, 1074, 1646, 2210, 2615, 2690, 2739, 3365
DraI TTTAAA 2 cut(s) 1762, 2424
DriI GACNNNNNGTC 1 cut(s) 1731
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 3232
Eam1105I GACNNNNNGTC 1 cut(s) 1731
EarI CTCTTC 1 cut(s) 3232
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 4 cut(s) 577, 1966, 2240, 2673
Eco147I AGGCCT 1 cut(s) 1686
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 2 cut(s) 1473, 1877
Eco32I GATATC 1 cut(s) 3520
Eco47I GGWCC 3 cut(s) 884, 1672, 2244
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 5 cut(s) 1641, 2703, 2981, 3245, 3437
Eco88I CYCGRG 2 cut(s) 612, 2099
Eco91I GGTNACC 1 cut(s) 2677
EcoICRI GAGCTC 1 cut(s) 235
EcoO65I GGTNACC 1 cut(s) 2677
EcoRI GAATTC 3 cut(s) 505, 2654, 3182
EcoRII CCWGG 5 cut(s) 886, 1444, 2395, 3019, 3294
EcoRV GATATC 1 cut(s) 3520
EcoT14I CCWWGG 4 cut(s) 577, 1966, 2240, 2673
EcoT22I ATGCAT 4 cut(s) 152, 472, 1389, 2863
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 4 cut(s) 577, 1966, 2240, 2673
FalI AAGNNNNNCTT 2 cut(s) 702, 734
FaqI GGGAC 2 cut(s) 855, 1719
FauI CCCGC 2 cut(s) 518, 579
FauNDI CATATG 1 cut(s) 539
FbaI TGATCA 1 cut(s) 625
FblI GTMKAC 1 cut(s) 2809
Fnu4HI GCNGC 3 cut(s) 54, 212, 324
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 3 cut(s) 54, 212, 324
FspBI CTAG 6 cut(s) 69, 698, 1298, 3542, 3558, 3567
GluI GCNGC 3 cut(s) 54, 212, 324
GsaI CCCAGC 1 cut(s) 1414
GsuI CTGGAG 2 cut(s) 1351, 3453
HaeIII GGCC 7 cut(s) 40, 165, 341, 696, 1686, 2193, 3409
HapII CCGG 2 cut(s) 3158, 3410
HgaI GACGC 1 cut(s) 17
HincII GTYRAC 3 cut(s) 484, 843, 1590
HindII GTYRAC 3 cut(s) 484, 843, 1590
HindIII AAGCTT 2 cut(s) 1982, 2741
HpaII CCGG 2 cut(s) 3158, 3410
HphI GGTGA 4 cut(s) 2645, 2689, 3302, 3433
Hpy166II GTNNAC 7 cut(s) 484, 545, 843, 1427, 1590, 1672, 2810
Hpy8I GTNNAC 7 cut(s) 484, 545, 843, 1427, 1590, 1672, 2810
HpyCH4III ACNGT 4 cut(s) 1058, 1380, 1431, 2315
HpyCH4IV ACGT 4 cut(s) 1453, 2122, 2331, 2603
HpyF10VI GCNNNNNNNGC 7 cut(s) 588, 1393, 2740, 3128, 3460, 3570, 3579
HpyF3I CTNAG 8 cut(s) 390, 1074, 1646, 2210, 2615, 2690, 2739, 3365
HpySE526I ACGT 4 cut(s) 1453, 2122, 2331, 2603
Ksp22I TGATCA 1 cut(s) 625
LmnI GCTCC 3 cut(s) 373, 773, 2639
Lsp1109I GCAGC 2 cut(s) 40, 310
LweI GCATC 8 cut(s) 479, 1054, 1374, 1584, 2279, 2390, 2848, 2870
MaeI CTAG 6 cut(s) 69, 698, 1298, 3542, 3558, 3567
MaeII ACGT 4 cut(s) 1453, 2122, 2331, 2603
MaeIII GTNAC 4 cut(s) 1285, 2197, 2660, 2677
MfeI CAATTG 3 cut(s) 57, 2175, 2905
MflI RGATCY 7 cut(s) 71, 196, 573, 835, 1349, 1742, 1924
MhlI GDGCHC 3 cut(s) 237, 370, 3153
MlsI TGGCCA 1 cut(s) 341
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 9 cut(s) 59, 1707, 2032, 2769, 2805, 3112, 3311, 3437, 3444
MmeI TCCRAC 1 cut(s) 1015
Mox20I TGGCCA 1 cut(s) 341
Mph1103I ATGCAT 4 cut(s) 152, 472, 1389, 2863
MroXI GAANNNNTTC 4 cut(s) 505, 717, 1419, 3003
MscI TGGCCA 1 cut(s) 341
MslI CAYNNNNRTG 4 cut(s) 363, 500, 1580, 3054
Msp20I TGGCCA 1 cut(s) 341
MspCI CTTAAG 3 cut(s) 1558, 1738, 3070
MspI CCGG 2 cut(s) 3158, 3410
MspR9I CCNGG 6 cut(s) 888, 1446, 2397, 3021, 3296, 3411
MunI CAATTG 3 cut(s) 57, 2175, 2905
Mva1269I GAATGC 5 cut(s) 513, 2642, 2712, 2724, 2939
MvaI CCWGG 5 cut(s) 888, 1446, 2397, 3021, 3296
MwoI GCNNNNNNNGC 7 cut(s) 588, 1393, 2740, 3128, 3460, 3570, 3579
NciI CCSGG 1 cut(s) 3411
NcoI CCATGG 1 cut(s) 577
NdeI CATATG 1 cut(s) 539
NlaIV GGNNCC 3 cut(s) 198, 215, 575
NmuCI GTSAC 3 cut(s) 1285, 2197, 2677
NsiI ATGCAT 4 cut(s) 152, 472, 1389, 2863
NspI RCATGY 2 cut(s) 362, 586
PaeI GCATGC 2 cut(s) 362, 586
PaeR7I CTCGAG 1 cut(s) 612
PagI TCATGA 2 cut(s) 517, 1471
PaqCI CACCTGC 1 cut(s) 1889
PceI AGGCCT 1 cut(s) 1686
PctI GAATGC 5 cut(s) 513, 2642, 2712, 2724, 2939
PdmI GAANNNNTTC 4 cut(s) 505, 717, 1419, 3003
PflMI CCANNNNNTGG 2 cut(s) 551, 3160
PfoI TCCNGGA 2 cut(s) 2395, 3019
PkrI GCNGC 3 cut(s) 55, 213, 325
PleI GAGTC 9 cut(s) 59, 1707, 2031, 2769, 2805, 3111, 3310, 3436, 3443
PpsI GAGTC 9 cut(s) 59, 1707, 2031, 2769, 2805, 3111, 3310, 3436, 3443
PshAI GACNNNNGTC 1 cut(s) 383
PshBI ATTAAT 1 cut(s) 2063
Psp124BI GAGCTC 1 cut(s) 237
Psp1406I AACGTT 1 cut(s) 2122
Psp6I CCWGG 5 cut(s) 886, 1444, 2395, 3019, 3294
PspEI GGTNACC 1 cut(s) 2677
PspFI CCCAGC 1 cut(s) 1410
PspGI CCWGG 5 cut(s) 886, 1444, 2395, 3019, 3294
PspN4I GGNNCC 3 cut(s) 198, 215, 575
PspPI GGNCC 6 cut(s) 39, 163, 884, 1672, 2244, 3407
PspXI VCTCGAGB 1 cut(s) 612
PstI CTGCAG 1 cut(s) 2222
PsuI RGATCY 7 cut(s) 71, 196, 573, 835, 1349, 1742, 1924
RsaI GTAC 2 cut(s) 2709, 2836
RsaNI GTAC 2 cut(s) 2708, 2835
RseI CAYNNNNRTG 4 cut(s) 363, 500, 1580, 3054
SacI GAGCTC 1 cut(s) 237
SatI GCNGC 3 cut(s) 54, 212, 324
Sau96I GGNCC 6 cut(s) 39, 163, 884, 1672, 2244, 3407
SchI GAGTC 9 cut(s) 59, 1707, 2032, 2769, 2805, 3112, 3311, 3437, 3444
ScrFI CCNGG 6 cut(s) 888, 1446, 2397, 3021, 3296, 3411
SduI GDGCHC 3 cut(s) 237, 370, 3153
SfaNI GCATC 8 cut(s) 479, 1054, 1374, 1584, 2279, 2390, 2848, 2870
SfcI CTRYAG 1 cut(s) 2218
Sfr274I CTCGAG 1 cut(s) 612
SinI GGWCC 3 cut(s) 884, 1672, 2244
SlaI CTCGAG 1 cut(s) 612
SmiMI CAYNNNNRTG 4 cut(s) 363, 500, 1580, 3054
SmlI CTYRAG 9 cut(s) 17, 612, 1558, 1738, 1913, 2018, 2744, 3070, 3383
SmoI CTYRAG 9 cut(s) 17, 612, 1558, 1738, 1913, 2018, 2744, 3070, 3383
SpeI ACTAGT 2 cut(s) 3557, 3566
SphI GCATGC 2 cut(s) 362, 586
SseBI AGGCCT 1 cut(s) 1686
SsiI CCGC 7 cut(s) 211, 511, 586, 793, 2482, 2572, 3369
SspI AATATT 2 cut(s) 1239, 2061
SspMI CTAG 6 cut(s) 69, 698, 1298, 3542, 3558, 3567
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 1686
StyD4I CCNGG 6 cut(s) 886, 1444, 2395, 3019, 3294, 3409
StyI CCWWGG 4 cut(s) 577, 1966, 2240, 2673
TaaI ACNGT 4 cut(s) 1058, 1380, 1431, 2315
TaiI ACGT 4 cut(s) 1456, 2125, 2334, 2606
TaqI TCGA 7 cut(s) 613, 1382, 1719, 2728, 3231, 3491, 3524
TaqII GACCGA 2 cut(s) 663, 1520
TatI WGTACW 1 cut(s) 2834
TauI GCSGC 1 cut(s) 214
TscAI CASTG 2 cut(s) 1135, 2847
TseFI GTSAC 3 cut(s) 1285, 2197, 2677
TseI GCWGC 2 cut(s) 53, 323
Tsp45I GTSAC 3 cut(s) 1285, 2197, 2677
TspGWI ACGGA 4 cut(s) 451, 1074, 3096, 3428
TspRI CASTG 2 cut(s) 1135, 2847
Van91I CCANNNNNTGG 2 cut(s) 551, 3160
Vha464I CTTAAG 3 cut(s) 1558, 1738, 3070
VpaK11BI GGWCC 3 cut(s) 884, 1672, 2244
VspI ATTAAT 1 cut(s) 2063
XbaI TCTAGA 1 cut(s) 68
XceI RCATGY 2 cut(s) 362, 586
XcmI CCANNNNNNNNNTGG 3 cut(s) 733, 1964, 3056
XhoI CTCGAG 1 cut(s) 612
XmiI GTMKAC 1 cut(s) 2809
XmnI GAANNNNTTC 4 cut(s) 505, 717, 1419, 3003
XspI CTAG 6 cut(s) 69, 698, 1298, 3542, 3558, 3567
Zsp2I ATGCAT 4 cut(s) 152, 472, 1389, 2863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.