RchiOBHm_Chr1g0322381

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
9747635 .. 9751888
4254 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55238

Sequence Viewer

Length: 3525 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGCGGGGCAGATACCCGCTATACTTTTACCGATCGTCTGTATTCCGCACTAGTTCAGAAAGGAATTCTCACCTTTATGGATGATCCAGAGCTTGAGAAAGGAAAATCCATTAGGCTTTTAGCTGCAATTGAGGACTCTAGATCTGCGATTGTCATTCTCTCAGGGGGCTATGCTTCTTCGACATGGTGCTTGGATGAACTCGTCAAGATTATTCAGTGCATGAAAGAGATGGGCCAACAAGTCCTCCCCGTCTTCTACTGGGTGGATCCTTCTGATGTGCGGCACCAAAGGCGAAGTTTTGAGCTCAAATGGAAAACCCATGTAGATGTGGAAGTGGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATGAGGACAGACTAAATGCGTGGAGAGCTGCTTTGACAGAGGTCGCCAATCTTTCTGGCTGGCATTTTACGGGTGGATATCCAACAAACCTAGTTGACGAAATTGTTAATCACATACTCAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATCGATTCCCGTGTTGATGATTTTTTAAGCAATTACATATATCCGCAGTTTGGTGGGGTGTGTTTTATAGGGATCCATGGCCTGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCGGACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTTCAAGAAAAACTTCTTTCCAGAATCCTGATGGCTAAAATTAAAAATATAGAGCATGAAGACACAGGAGCTGCTATGATTAAAAGGCGGTTCTGCAGAACAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATCATCATAACCACCACAGATATTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGGTGAAGCACTTCAACTTTTGAGTTTGAGGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGGATCTGTGCTACCATATTCTAGGGTATGCTCGGGGGCTTCCATTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCGCTGATAAATGGGCAAGTGCAATAGATAGGCTAAAGAAGACACCAGATAAACGCATTATTGACGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTGCATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAACTAGACCCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAGAAATACGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTACATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTTGTAACGTGGACCTTCCCAAAGGCCTCACCTGTCTTTCTAATTCCTTGAGACTTCTTGAATGGACTGGGTGTCCCTTAAGATCTCTCCCACAAAACTTTGAAGCCGATGAACTTGTTGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCGTTTGGTAAGAATCCATCAATCCCTTGCATTTCTCAAAAAGCTTATTGTGCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGACTGCCAAGTAGAATTGAAATGGAGTCTCTTGAATCATTAATTCTTTCTAACTGTTCAAAAGTTAAGAAGATTCCTGAGTTTGTTGGAAATATGGAATGTTTGTTGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGATTCAATTGAACAGCTGAGTGGCCTTGTGTCATTGAATCTACGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAATTGAAGTCTATTGAAGATCTTAATCTTTCTGGATGCTTGAACCTCGGCAAACCTCAGGTAAATGTGGGGGTGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCGTCTACCCATGATCGCAAGAAAAAAGGGAGAGGTCCAATCTTTCAAGGATGCAAAGTAGTTTGGCGATCTTTCAAAAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAAAGAGCTTCCACTTGCCTATATCACAAAACGTGAATGCAGAGCCAAAGAGCTTCCACTTGCCTATATCACAAAACGTGAATGCAGAGCCAAAGAGTTTCCACTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGTATTTGCCAATGAATTTGGTTACTTTCCCTCTTTGGTAACCTTGAATCTAAGTGGGAACAATTTTGTTCGTCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAATGGTTCAGCCATCGGAGTGTTGGGCGCTCTGTAAGTGTATCTCTACCTGCACATTGGAATAACAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTCCATGAGCAACATCGGAATGAAAAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAGAATTGGAAGTATATGGCAGACATCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTCGGTACAGAGTGGTGGCATAACAGTTGCAGTAAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGAAGGTGAAGGAATGTGGAGTCCGTCAATTGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1174

Amino Acids

133.27

Weight (kDa)

7.0

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 190 1.5e-41 TIR domain
TIR_2 PF13676 14 - 105 3.2e-11 TIR domain
NB-ARC PF00931 218 - 370 1.7e-15 NB-ARC domain
NACHT PF05729 222 - 316 2.9e-06 NACHT domain
WHD_ROQ1 PF23282 437 - 508 1.3e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 606 - 624 4.9e-07 Leucine Rich Repeat
LRR_13 PF23286 697 - 781 8.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 708 - 776 6.6e-10 Leucine-rich repeat region
C-JID PF20160 1030 - 1173 7.6e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1931
Acc36I ACCTGC 3 cut(s) 752, 1931, 3166
AccB1I GGYRCC 1 cut(s) 336
AccB7I CCANNNNNTGG 1 cut(s) 1618
AccI GTMKAC 3 cut(s) 557, 2426, 2881
AciI CCGC 7 cut(s) 57, 70, 99, 334, 635, 676, 883
AclWI GGATC 8 cut(s) 131, 314, 327, 658, 671, 933, 1143, 1377
AcsI RAATTY 8 cut(s) 117, 720, 1675, 1873, 2726, 2938, 3254, 3359
AcuI CTGAAG 5 cut(s) 1604, 2775, 3053, 3317, 3509
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 2908, 3213, 3424
AfeI AGCGCT 1 cut(s) 1219
AfiI CCNNNNNNNGG 4 cut(s) 641, 1415, 1618, 2657
AflII CTTAAG 1 cut(s) 1780
AhdI GACNNNNNGTC 1 cut(s) 1773
AhlI ACTAGT 1 cut(s) 103
AjiI CACGTC 1 cut(s) 1282
AjnI CCWGG 4 cut(s) 976, 1534, 3091, 3481
AjuI GAANNNNNNNTTGG 2 cut(s) 2813, 2845
Alw21I GWGCWC 1 cut(s) 360
AlwI GGATC 8 cut(s) 131, 314, 327, 658, 671, 933, 1143, 1377
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 3 cut(s) 702, 1164, 2060
Aor51HI AGCGCT 1 cut(s) 1219
AoxI GGCC 6 cut(s) 286, 670, 784, 1726, 2233, 3479
ApeKI GCWGC 3 cut(s) 176, 452, 866
ApoI RAATTY 8 cut(s) 117, 720, 1675, 1873, 2726, 2938, 3254, 3359
AseI ATTAAT 1 cut(s) 2105
Asp700I GAANNNNTTC 7 cut(s) 595, 807, 1077, 1509, 1603, 3038, 3075
AspLEI GCGC 2 cut(s) 1220, 3138
AspS9I GGNCC 6 cut(s) 286, 741, 974, 1714, 2456, 3479
AsuHPI GGTGA 7 cut(s) 115, 1082, 1723, 2380, 2717, 3374, 3505
AvaI CYCGRG 3 cut(s) 702, 1164, 2060
AvaII GGWCC 4 cut(s) 741, 974, 1714, 2456
AxyI CCTNAGG 1 cut(s) 2352
BamHI GGATCC 2 cut(s) 319, 663
BanI GGYRCC 1 cut(s) 336
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 2 cut(s) 1184, 3405
BbsI GAAGAC 8 cut(s) 298, 861, 1262, 1572, 1685, 2270, 2777, 3041
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 3 cut(s) 163, 439, 853
BcgI CGANNNNNNTGC 2 cut(s) 2041, 2075
BciT130I CCWGG 4 cut(s) 978, 1536, 3093, 3483
BcuI ACTAGT 1 cut(s) 103
BfaI CTAG 8 cut(s) 104, 192, 515, 788, 1154, 1388, 1403, 2885
BfmI CTRYAG 2 cut(s) 889, 2260
BfoI RGCGCY 2 cut(s) 1221, 3139
BfrI CTTAAG 1 cut(s) 1780
BfuAI ACCTGC 3 cut(s) 752, 1931, 3166
BglI GCCNNNNNGGC 1 cut(s) 678
BglII AGATCT 5 cut(s) 194, 1439, 1784, 1966, 2314
BisI GCNGC 4 cut(s) 177, 335, 453, 867
BlsI GCNGC 4 cut(s) 178, 336, 454, 868
Bme1390I CCNGG 4 cut(s) 978, 1536, 3093, 3483
Bme18I GGWCC 4 cut(s) 741, 974, 1714, 2456
BmeRI GACNNNNNGTC 1 cut(s) 1773
BmeT110I CYCGRG 3 cut(s) 702, 1164, 2060
BmgBI CACGTC 1 cut(s) 1282
BmgT120I GGNCC 6 cut(s) 286, 741, 974, 1714, 2456, 3479
BmiI GGNNCC 3 cut(s) 321, 338, 665
BmrFI CCNGG 4 cut(s) 978, 1536, 3093, 3483
BmrI ACTGGG 2 cut(s) 322, 1779
BmsI GCATC 7 cut(s) 1027, 1464, 2321, 2462, 2920, 2942, 2996
BmuI ACTGGG 2 cut(s) 322, 1779
BoxI GACNNNNGTC 1 cut(s) 464
BpiI GAAGAC 8 cut(s) 298, 861, 1262, 1572, 1685, 2270, 2777, 3041
BpuEI CTTGAG 5 cut(s) 167, 527, 1771, 1976, 2837
Bsa29I ATCGAT 1 cut(s) 594
BsaBI GATNNNNATC 3 cut(s) 924, 1368, 2319
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1563, 1919
BsaJI CCNNGG 4 cut(s) 667, 977, 1612, 2341
BsaWI WCCGGW 1 cut(s) 738
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 4 cut(s) 641, 1415, 1618, 2657
Bse1I ACTGG 4 cut(s) 317, 971, 1311, 1774
Bse21I CCTNAGG 1 cut(s) 2352
Bse8I GATNNNNATC 3 cut(s) 924, 1368, 2319
BseBI CCWGG 4 cut(s) 978, 1536, 3093, 3483
BseCI ATCGAT 1 cut(s) 594
BseDI CCNNGG 4 cut(s) 667, 977, 1612, 2341
BseGI GGATG 8 cut(s) 139, 253, 1315, 1982, 2336, 2477, 3011, 3334
BseJI GATNNNNATC 3 cut(s) 924, 1368, 2319
BseLI CCNNNNNNNGG 4 cut(s) 641, 1415, 1618, 2657
BseMII CTCAG 5 cut(s) 228, 2133, 2219, 2366, 2678
BseNI ACTGG 4 cut(s) 317, 971, 1311, 1774
BseXI GCAGC 3 cut(s) 163, 439, 853
BseYI CCCAGC 1 cut(s) 1500
BsgI GTGCAG 1 cut(s) 3144
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 6 cut(s) 288, 672, 786, 1728, 2235, 3481
BshNI GGYRCC 1 cut(s) 336
BshVI ATCGAT 1 cut(s) 594
BsiEI CGRYCG 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 360
BsiHKCI CYCGRG 3 cut(s) 702, 1164, 2060
BsiSI CCGG 1 cut(s) 739
BslFI GGGAC 2 cut(s) 1098, 1761
BslI CCNNNNNNNGG 4 cut(s) 641, 1415, 1618, 2657
BsmFI GGGAC 2 cut(s) 1098, 1761
BsmI GAATGC 2 cut(s) 2584, 2629
BsnI GGCC 6 cut(s) 288, 672, 786, 1728, 2235, 3481
Bso31I GGTCTC 2 cut(s) 1563, 1919
BsoBI CYCGRG 3 cut(s) 702, 1164, 2060
Bsp1286I GDGCHC 1 cut(s) 360
Bsp19I CCATGG 1 cut(s) 667
BspACI CCGC 7 cut(s) 57, 70, 99, 334, 635, 676, 883
BspANI GGCC 6 cut(s) 288, 672, 786, 1728, 2235, 3481
BspCNI CTCAG 5 cut(s) 227, 2134, 2220, 2365, 2679
BspDI ATCGAT 1 cut(s) 594
BspHI TCATGA 1 cut(s) 1561
BspLI GGNNCC 3 cut(s) 321, 338, 665
BspMAI CTGCAG 2 cut(s) 893, 2264
BspMI ACCTGC 3 cut(s) 752, 1931, 3166
BspPI GGATC 8 cut(s) 131, 314, 327, 658, 671, 933, 1143, 1377
BspT107I GGYRCC 1 cut(s) 336
BspTI CTTAAG 1 cut(s) 1780
BspTNI GGTCTC 2 cut(s) 1563, 1919
BsrI ACTGG 4 cut(s) 317, 971, 1311, 1774
BssECI CCNNGG 4 cut(s) 667, 977, 1612, 2341
BssNAI GTATAC 2 cut(s) 558, 2882
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1184, 3405
BssT1I CCWWGG 2 cut(s) 667, 1612
Bst1107I GTATAC 2 cut(s) 558, 2882
Bst2BI CACGAG 2 cut(s) 1184, 3405
Bst2UI CCWGG 4 cut(s) 978, 1536, 3093, 3483
Bst4CI ACNGT 7 cut(s) 1067, 1470, 1521, 2120, 2378, 3176, 3443
Bst6I CTCTTC 4 cut(s) 19, 801, 1580, 3304
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1780
BstAPI GCANNNNNTGC 1 cut(s) 1483
BstC8I GCNNGC 3 cut(s) 485, 674, 1345
BstDEI CTNAG 9 cut(s) 47, 214, 1688, 2142, 2228, 2352, 2687, 2762, 2811
BstDSI CCRYGG 1 cut(s) 667
BstEII GGTNACC 1 cut(s) 2749
BstF5I GGATG 8 cut(s) 139, 253, 1315, 1982, 2336, 2477, 3011, 3334
BstH2I RGCGCY 2 cut(s) 1221, 3139
BstHHI GCGC 2 cut(s) 1220, 3138
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 7 cut(s) 343, 449, 678, 1483, 2032, 2812, 3200
BstNI CCWGG 4 cut(s) 978, 1536, 3093, 3483
BstPAI GACNNNNGTC 1 cut(s) 464
BstPI GGTNACC 1 cut(s) 2749
BstSCI CCNGG 4 cut(s) 976, 1534, 3091, 3481
BstSFI CTRYAG 2 cut(s) 889, 2260
BstV1I GCAGC 3 cut(s) 163, 439, 853
BstV2I GAAGAC 8 cut(s) 298, 861, 1262, 1572, 1685, 2270, 2777, 3041
BstX2I RGATCY 8 cut(s) 194, 319, 663, 1135, 1439, 1784, 1966, 2314
BstYI RGATCY 8 cut(s) 194, 319, 663, 1135, 1439, 1784, 1966, 2314
BstZ17I GTATAC 2 cut(s) 558, 2882
Bsu15I ATCGAT 1 cut(s) 594
Bsu36I CCTNAGG 1 cut(s) 2352
BsuRI GGCC 6 cut(s) 288, 672, 786, 1728, 2235, 3481
BsuTUI ATCGAT 1 cut(s) 594
BtgI CCRYGG 1 cut(s) 667
BtrI CACGTC 1 cut(s) 1282
BtsCI GGATG 8 cut(s) 139, 253, 1315, 1982, 2336, 2477, 3011, 3334
BtsIMutI CAGTG 2 cut(s) 275, 2912
BveI ACCTGC 3 cut(s) 752, 1931, 3166
Cac8I GCNNGC 3 cut(s) 485, 674, 1345
CaiI CAGNNNCTG 1 cut(s) 866
CciI TCATGA 1 cut(s) 1561
CfoI GCGC 2 cut(s) 1220, 3138
Cfr13I GGNCC 6 cut(s) 286, 741, 974, 1714, 2456, 3479
ClaI ATCGAT 1 cut(s) 594
Csp6I GTAC 3 cut(s) 2907, 3212, 3423
CviQI GTAC 3 cut(s) 2907, 3212, 3423
DdeI CTNAG 9 cut(s) 47, 214, 1688, 2142, 2228, 2352, 2687, 2762, 2811
DriI GACNNNNNGTC 1 cut(s) 1773
Eam1104I CTCTTC 4 cut(s) 19, 801, 1580, 3304
Eam1105I GACNNNNNGTC 1 cut(s) 1773
EarI CTCTTC 4 cut(s) 19, 801, 1580, 3304
Ecl136II GAGCTC 1 cut(s) 358
Eco130I CCWWGG 2 cut(s) 667, 1612
Eco147I AGGCCT 1 cut(s) 1728
Eco24I GRGCYC 1 cut(s) 360
Eco31I GGTCTC 2 cut(s) 1563, 1919
Eco32I GATATC 1 cut(s) 503
Eco47I GGWCC 4 cut(s) 741, 974, 1714, 2456
Eco47III AGCGCT 1 cut(s) 1219
Eco53kI GAGCTC 1 cut(s) 358
Eco57I CTGAAG 5 cut(s) 1604, 2775, 3053, 3317, 3509
Eco81I CCTNAGG 1 cut(s) 2352
Eco88I CYCGRG 3 cut(s) 702, 1164, 2060
Eco91I GGTNACC 1 cut(s) 2749
EcoICRI GAGCTC 1 cut(s) 358
EcoO65I GGTNACC 1 cut(s) 2749
EcoRI GAATTC 2 cut(s) 117, 3254
EcoRII CCWGG 4 cut(s) 976, 1534, 3091, 3481
EcoRV GATATC 1 cut(s) 503
EcoT14I CCWWGG 2 cut(s) 667, 1612
EcoT22I ATGCAT 2 cut(s) 1479, 2935
EcoT38I GRGCYC 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 667, 1612
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 2 cut(s) 1098, 1761
FauI CCCGC 3 cut(s) 50, 77, 669
FblI GTMKAC 3 cut(s) 557, 2426, 2881
Fnu4HI GCNGC 4 cut(s) 177, 335, 453, 867
FokI GGATG 8 cut(s) 146, 260, 1322, 1989, 2343, 2484, 3018, 3321
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 4 cut(s) 177, 335, 453, 867
FspBI CTAG 8 cut(s) 104, 192, 515, 788, 1154, 1388, 1403, 2885
GlaI GCGC 2 cut(s) 1219, 3137
GluI GCNGC 4 cut(s) 177, 335, 453, 867
GsaI CCCAGC 1 cut(s) 1504
HaeII RGCGCY 2 cut(s) 1221, 3139
HaeIII GGCC 6 cut(s) 288, 672, 786, 1728, 2235, 3481
HapII CCGG 1 cut(s) 739
HhaI GCGC 2 cut(s) 1220, 3138
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 1218, 3136
HinP1I GCGC 2 cut(s) 1218, 3136
HincII GTYRAC 5 cut(s) 9, 520, 574, 933, 1632
HindII GTYRAC 5 cut(s) 9, 520, 574, 933, 1632
HindIII AAGCTT 3 cut(s) 2024, 2813, 3239
HpaII CCGG 1 cut(s) 739
HphI GGTGA 7 cut(s) 115, 1082, 1723, 2380, 2717, 3374, 3505
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 7 cut(s) 1067, 1470, 1521, 2120, 2378, 3176, 3443
HpyCH4IV ACGT 5 cut(s) 1281, 1543, 1710, 2574, 2619
HpyF10VI GCNNNNNNNGC 7 cut(s) 343, 449, 678, 1483, 2032, 2812, 3200
HpyF3I CTNAG 9 cut(s) 47, 214, 1688, 2142, 2228, 2352, 2687, 2762, 2811
HpySE526I ACGT 5 cut(s) 1281, 1543, 1710, 2574, 2619
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 1218, 3136
LmnI GCTCC 1 cut(s) 863
Lsp1109I GCAGC 3 cut(s) 163, 439, 853
LweI GCATC 7 cut(s) 1027, 1464, 2321, 2462, 2920, 2942, 2996
MaeI CTAG 8 cut(s) 104, 192, 515, 788, 1154, 1388, 1403, 2885
MaeII ACGT 5 cut(s) 1281, 1543, 1710, 2574, 2619
MaeIII GTNAC 4 cut(s) 1375, 1706, 2732, 2749
MfeI CAATTG 4 cut(s) 180, 2217, 2977, 3515
MflI RGATCY 8 cut(s) 194, 319, 663, 1135, 1439, 1784, 1966, 2314
MhlI GDGCHC 1 cut(s) 360
MlyI GAGTC 3 cut(s) 182, 2099, 3516
MmeI TCCRAC 3 cut(s) 530, 1482, 2131
Mph1103I ATGCAT 2 cut(s) 1479, 2935
MroXI GAANNNNTTC 7 cut(s) 595, 807, 1077, 1509, 1603, 3038, 3075
MslI CAYNNNNRTG 5 cut(s) 128, 378, 1622, 3126, 3231
MspA1I CMGCKG 2 cut(s) 57, 2227
MspCI CTTAAG 1 cut(s) 1780
MspI CCGG 1 cut(s) 739
MspR9I CCNGG 4 cut(s) 978, 1536, 3093, 3483
MunI CAATTG 4 cut(s) 180, 2217, 2977, 3515
Mva1269I GAATGC 2 cut(s) 2584, 2629
MvaI CCWGG 4 cut(s) 978, 1536, 3093, 3483
MwoI GCNNNNNNNGC 7 cut(s) 343, 449, 678, 1483, 2032, 2812, 3200
NcoI CCATGG 1 cut(s) 667
NlaIV GGNNCC 3 cut(s) 321, 338, 665
NmeAIII GCCGAG 1 cut(s) 2322
NmuCI GTSAC 1 cut(s) 1375
NsiI ATGCAT 2 cut(s) 1479, 2935
PaeR7I CTCGAG 2 cut(s) 702, 2060
PagI TCATGA 1 cut(s) 1561
PaqCI CACCTGC 1 cut(s) 1931
PceI AGGCCT 1 cut(s) 1728
PctI GAATGC 2 cut(s) 2584, 2629
PdmI GAANNNNTTC 7 cut(s) 595, 807, 1077, 1509, 1603, 3038, 3075
PflFI GACNNNGTC 1 cut(s) 1409
PflMI CCANNNNNTGG 1 cut(s) 1618
PfoI TCCNGGA 1 cut(s) 3091
PkrI GCNGC 4 cut(s) 178, 336, 454, 868
Ple19I CGATCG 1 cut(s) 88
PleI GAGTC 3 cut(s) 182, 2098, 3515
PpsI GAGTC 3 cut(s) 182, 2098, 3515
PshAI GACNNNNGTC 1 cut(s) 464
PshBI ATTAAT 1 cut(s) 2105
Psp124BI GAGCTC 1 cut(s) 360
Psp6I CCWGG 4 cut(s) 976, 1534, 3091, 3481
PspEI GGTNACC 1 cut(s) 2749
PspFI CCCAGC 1 cut(s) 1500
PspGI CCWGG 4 cut(s) 976, 1534, 3091, 3481
PspN4I GGNNCC 3 cut(s) 321, 338, 665
PspPI GGNCC 6 cut(s) 286, 741, 974, 1714, 2456, 3479
PspXI VCTCGAGB 1 cut(s) 702
PstI CTGCAG 2 cut(s) 893, 2264
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 8 cut(s) 194, 319, 663, 1135, 1439, 1784, 1966, 2314
PsyI GACNNNGTC 1 cut(s) 1409
PvuI CGATCG 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 2227
RsaI GTAC 3 cut(s) 2908, 3213, 3424
RsaNI GTAC 3 cut(s) 2907, 3212, 3423
RseI CAYNNNNRTG 5 cut(s) 128, 378, 1622, 3126, 3231
SacI GAGCTC 1 cut(s) 360
SatI GCNGC 4 cut(s) 177, 335, 453, 867
Sau96I GGNCC 6 cut(s) 286, 741, 974, 1714, 2456, 3479
SchI GAGTC 3 cut(s) 182, 2099, 3516
ScrFI CCNGG 4 cut(s) 978, 1536, 3093, 3483
SduI GDGCHC 1 cut(s) 360
SfaNI GCATC 7 cut(s) 1027, 1464, 2321, 2462, 2920, 2942, 2996
SfcI CTRYAG 2 cut(s) 889, 2260
Sfr274I CTCGAG 2 cut(s) 702, 2060
SinI GGWCC 4 cut(s) 741, 974, 1714, 2456
SlaI CTCGAG 2 cut(s) 702, 2060
SmiMI CAYNNNNRTG 5 cut(s) 128, 378, 1622, 3126, 3231
SmlI CTYRAG 8 cut(s) 146, 542, 702, 1750, 1780, 1955, 2060, 2816
SmoI CTYRAG 8 cut(s) 146, 542, 702, 1750, 1780, 1955, 2060, 2816
SpeI ACTAGT 1 cut(s) 103
SseBI AGGCCT 1 cut(s) 1728
SsiI CCGC 7 cut(s) 57, 70, 99, 334, 635, 676, 883
SspI AATATT 2 cut(s) 1329, 3103
SspMI CTAG 8 cut(s) 104, 192, 515, 788, 1154, 1388, 1403, 2885
SstI GAGCTC 1 cut(s) 360
StuI AGGCCT 1 cut(s) 1728
StyD4I CCNGG 4 cut(s) 976, 1534, 3091, 3481
StyI CCWWGG 2 cut(s) 667, 1612
TaaI ACNGT 7 cut(s) 1067, 1470, 1521, 2120, 2378, 3176, 3443
TaiI ACGT 5 cut(s) 1284, 1546, 1713, 2577, 2622
TaqI TCGA 6 cut(s) 233, 594, 703, 1472, 2061, 3303
TatI WGTACW 2 cut(s) 2906, 3211
TauI GCSGC 1 cut(s) 337
TscAI CASTG 2 cut(s) 275, 2919
TseFI GTSAC 1 cut(s) 1375
TseI GCWGC 3 cut(s) 176, 452, 866
Tsp45I GTSAC 1 cut(s) 1375
TspGWI ACGGA 2 cut(s) 541, 3500
TspRI CASTG 2 cut(s) 275, 2919
Tth111I GACNNNGTC 1 cut(s) 1409
Van91I CCANNNNNTGG 1 cut(s) 1618
Vha464I CTTAAG 1 cut(s) 1780
VpaK11BI GGWCC 4 cut(s) 741, 974, 1714, 2456
VspI ATTAAT 1 cut(s) 2105
XapI RAATTY 8 cut(s) 117, 720, 1675, 1873, 2726, 2938, 3254, 3359
XbaI TCTAGA 1 cut(s) 191
XcmI CCANNNNNNNNNTGG 2 cut(s) 823, 3128
XhoI CTCGAG 2 cut(s) 702, 2060
XmiI GTMKAC 3 cut(s) 557, 2426, 2881
XmnI GAANNNNTTC 7 cut(s) 595, 807, 1077, 1509, 1603, 3038, 3075
XspI CTAG 8 cut(s) 104, 192, 515, 788, 1154, 1388, 1403, 2885
Zsp2I ATGCAT 2 cut(s) 1479, 2935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.