MD10G1007300.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000105
Physical Location & Seq
Reverse (-)
1125185 .. 1129251
4067 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1007300.v1.1.491

Sequence Viewer

Length: 3360 bp
ATGGGATCCACGGCGACTGCGCAAACAACTGATTACGATGTCTTCCTCAGTTTCAGTGGCGAAGACACTCGCAGGAATTTCGCAGACCAACTACGTGCTGCTCTGAGTAACCGAGGAGTCGTCGTATTTGGAGATGATGAACTTTATCGGGGAATTAAAAGTTCAAAAATTTCAATTGTCATTCTTTCAAGAAAGTATGCCAGGTCAACATGGTGCTTGGATGAGCTTCAGCAAATTATGGAATGCAAGGAAGTGGCTCGCCAGATAGTTTTGCCTGTCTTCTATGATGTGAGCCCATCCGAGGTACGAAAACAGACAGGCGATTTCGGTGACTTTTTTGCCGAGCATGAAGGACGTTTCAATGACAATATCGACAAAGTGCGCGCCTGGAGGGATGCTCTTCGTCAATTAGCCAATCTGTCTGGATTTGACCTATACAAGGACGAGGATGGGTATGAGGCACAATTTATCCAACGAATTGTTCAATGGGTTTTGAATACATTGCAAACTCTGCCATCTTATCTTGAGGACCTAGTGGGAATTGACTCAAGAGTAGAGGAACTAAATAGGCTTTTGGACATCAAATCTAATGATGTTCGCTTCATAGGGATATGCGGAATTGGTGGGATGGGCAAGACAACTATCGCCAGAGTGGTTTTTGACAACATTTCCAATGAATTTGAAATTTTCACCTGGATTACTTGTGGGCTAAGCGAAACACTGGGTCATCTGCAATTGCAAAAGAAACTTCTTTATGAGGCTTTGGGGCAAGGCATAGATGTTCCAAATGTTAACGAGGGAGCCATAATGATAAAGAACATTCTATGTAAGAGAAAGGTTCTTCTCATTTTAGACGATGTTTTCGACTTAGACCAACTGAAATCATTGGGTGGAAACAAAGAATGGTTTGGTTTGGGGAGCAGAGTCATCATTACAACTAGAAATGAGAATTTGCTAAGAGAGCATGGTGTAGAAAGCATATTTGAAGTTAAGGGATTAAGTGAGGGTGAGTCGCGTCAGCTCTTCAATATTCACGCCTTTAAAAGTGATGAACCCCGAGAATATTATTTGGATCTGTCTAAACGGGTGGTTCATTATGCTGGAGGCGTTCCTTTAGCAATCAAGGTTTTGGGATCCTCTCTGTTCCACAGAAGTCCGGGAGAGTGGCGTAATGCATTGGAAAAGCTGAAGCATGTTCCTGATAAAAATGTTTCTCAAGCACTTGAAATAAGTTATCATGGACTGGCGGACAATGAGAAAAGAATGTTTCTAGATATTGCTTGTTTCTTTCAAGGAATGGACAAGGAACGAGTGGTAGAGATACTAGATTGTTTTGGATTTAATCCGAAGGATGGGATGGAAGCTCTCTTTGAGAAATCTCTCATAACTATTTCAAATAACAAAGTGTTGATGCATGGGTTGATACAAGAATTGGGACGACAATTGGTACGCAGAGATGCTGTTGAGGATCCTGGAGAGCAAAGCAGGTTGTGGCTTCATGATGATGTCATTCATGTATTGAGGAATAATACGGGTACAAATGCAGTTGAAGGTATAGCCTTAGACTTCCCTAAATTGAAAGAGGCATGTTGGGACTTGGAGGCCTTCTCGACGATGCATAATCTCAGATTTCTCCAAATTCATAATCTGCGAATGACCCAAGGCCCAAAAAAACTTTCTAATGCCTTAAAGTTTCTTGAATGGAGTGGGTATCCTTCAAAATTTCTCCCACGAGAATTTGAACTTGAGCAACTTTGTGAAGTTAACTTGTCCCATAGCAGCATTGGACAACTTTGGAATAGAACAAAGTGCTTAGGCAATCTGAAATTCGTTAATGTTAGCTACTCCCAGAACCTGACCAGGACCCCGGACTTTACTGTTACTCCAAATCTTCATAGATTAATTCTTGAAGGCTGTACAAACTTGGTGATGATCGACCCATCCATTGCTGAGCTCAAAAGGCTTATTTTCTTAAATCTTAAGGACTGCAGAAGTCTTAAACGCCTTCCCAAATGTTTTGAAATGGAAAGTCTTCAAATTCTTATTCTTTCTGGTTGTTCAAAAATTAATAAAGTTCCAGAATTTGTGAGACCTATGGAAAACTTGTTGGAACTTTATTTAGATAAGACTGCTGTAGAAGAATTACCTTCTTCGATCGAATGTCTGACTGGCCTTACTTTATTAAACCTGAGAGATTGCAGAAATCTCAGTTCCCTTGCAAGCAGTACTTGCCAGTTGCGTTCCCTCAAAAGTCTCAATCTCAGTGGATGCTCAAAGCTTGAAGAACTGCCAAAAAACTTGGGAAATATGGTTTGTTTGAAGGAGCTTGATGTGGGCGGTACCTCTATTAATGATCTACCATCCTCCATTTCCCGCCTGGAAAATCTTGAATTGTTATCCATTTGTGGTTGCAAATCGATGCGAAGAAAGAAGACAAGTCTACTGAGTTTGCTGTTGCCGACCACAGATTCTGGTTTGCTTTCTTTGACAGTGCTGAATCTCAGTGACTGTAATCTTGAGGAGGTAACAATCCTGGAAAATCTTGGCTGCTTGTCCTCAATAGTATCGCTTAATCTAAGTAAAAACAGTTTTGTTAGTCTTCCCAAGAGCATCACAGAGCTGTCTAAGCTTCAGATTTTGAATTTGGGTGGTTGTAAGTGTCTTAAAAAATTGCCGGACCTTTCAGCAGAATTGAACTTGAGTGTTGAGGGAAAAGGCAGTTTTTCACGGGAAAGGCTGTCATCATGTTTCAGTTTTATTAATTGCTTTGAAGTGGTTGACGACCAAGGCTGCAACAATGTAGCATTTGCAGCGTTAAGGAGATTCCTTGAGGGAATCCCTTACGTAGGGAATAATTTTGAAGCTATATATCCTGGCAGTGAAATTTCTGAGTGGTTTGGTTATCGAAGTGAGAGGCCTGTGGTAAGCGTGGAGCTCCCTCAACACTGGTATAAGAACGAGTGGATGGGATATGCATTGTGTGCTGTTTTCACTCTCCGTCGACGACTCCCACCTAATCTGCTTGGTAAATGGAACTACGGAACCCATACCACTGCACATGGTCTTCGTTGCGAAGTTAAGCCTGGGAATTTGGGTGTAGGTGGCTGGTGTCCTTCCTTCGCCTGCAGCGAAGAATTAGGGCAAATTGAGAAAGAGCACATTTGGCTATCCTTTGTATCTGGTGAATACTTTGGTACAACATGGCAAGACAGTTGTCATCACCTTGAGTTTACATTTAAAACCTTAGGCTCTGGTTTGGAGGTGAAGAGGTGCGGAGTCCGTCTCATCTACGAGAGTGATTTGGTTGGATTAAAGCAACCTTCATCTAGAGGAAACAAGCAAAACAAGCAACTCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1120

Amino Acids

126.3

Weight (kDa)

6.17

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 174 9.3e-40 TIR domain
TIR_2 PF13676 14 - 113 1.1e-12 TIR domain
NB-ARC PF00931 184 - 349 1.5e-22 NB-ARC domain
WHD_ROQ1 PF23282 417 - 485 9.3e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 676 - 762 3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_R13L1-DRL21 PF25019 689 - 757 9.4e-06 R13L1/DRL21 LRRs
LRR_14 PF23598 725 - 810 5.5e-10 Leucine-rich repeat region
LRR_13 PF23286 760 - 895 9.7e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 828 - 895 7.6e-07 Leucine-rich repeat region
C-JID PF20160 958 - 1100 4e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 21
Acc36I ACCTGC 1 cut(s) 1476
Acc65I GGTACC 1 cut(s) 2339
AccB1I GGYRCC 1 cut(s) 2339
AccI GTMKAC 2 cut(s) 2440, 3001
AccII CGCG 2 cut(s) 384, 1015
AciI CCGC 5 cut(s) 615, 1247, 2337, 2374, 3273
AclWI GGATC 6 cut(s) 13, 1080, 1128, 1141, 1463, 1476
AcuI CTGAAG 3 cut(s) 212, 1208, 2615
AfaI GTAC 7 cut(s) 306, 1449, 1537, 1918, 2227, 2341, 3196
AfiI CCNNNNNNNGG 4 cut(s) 301, 439, 1352, 2846
AflII CTTAAG 1 cut(s) 1979
AjnI CCWGG 9 cut(s) 200, 386, 692, 1471, 1859, 2376, 2532, 2872, 3082
AjuI GAANNNNNNNTTGG 4 cut(s) 465, 497, 1660, 1692
Alw21I GWGCWC 3 cut(s) 1956, 2937, 3159
Alw26I GTCTC 3 cut(s) 2083, 2258, 3287
AlwI GGATC 6 cut(s) 13, 1080, 1128, 1141, 1463, 1476
AlwNI CAGNNNCTG 3 cut(s) 1855, 2471, 2508
Ama87I CYCGRG 1 cut(s) 1056
AoxI GGCC 4 cut(s) 1602, 1663, 2170, 2915
ApeKI GCWGC 6 cut(s) 98, 1779, 2547, 2790, 2810, 3126
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
AseI ATTAAT 4 cut(s) 1901, 2067, 2349, 2760
Asp700I GAANNNNTTC 1 cut(s) 2031
Asp718I GGTACC 1 cut(s) 2339
AspLEI GCGC 3 cut(s) 22, 384, 386
AspS9I GGNCC 4 cut(s) 529, 1664, 1863, 2677
AsuC2I CCSGG 2 cut(s) 1158, 1868
AsuHPI GGTGA 7 cut(s) 341, 682, 1019, 1939, 3194, 3212, 3274
AvaI CYCGRG 1 cut(s) 1056
AvaII GGWCC 3 cut(s) 529, 1863, 2677
AxyI CCTNAGG 1 cut(s) 3244
BamHI GGATCC 3 cut(s) 5, 1133, 1468
BanI GGYRCC 1 cut(s) 2339
BanII GRGCYC 3 cut(s) 296, 1956, 2937
BauI CACGAG 1 cut(s) 1731
BbsI GAAGAC 7 cut(s) 34, 69, 271, 2024, 2438, 2591, 3056
Bbv12I GWGCWC 3 cut(s) 1956, 2937, 3159
BbvI GCAGC 6 cut(s) 85, 1791, 2534, 2777, 2822, 3138
BccI CCATC 9 cut(s) 304, 443, 523, 622, 1346, 1351, 1948, 2368, 2959
BceAI ACGGC 1 cut(s) 27
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 9 cut(s) 202, 388, 694, 1473, 1861, 2378, 2534, 2874, 3084
BciVI GTATCC 1 cut(s) 1722
BcnI CCSGG 2 cut(s) 1158, 1868
BcoDI GTCTC 3 cut(s) 2083, 2258, 3287
BfaI CTAG 5 cut(s) 533, 939, 1271, 1325, 3327
BfmI CTRYAG 3 cut(s) 1987, 2133, 3124
BfrI CTTAAG 1 cut(s) 1979
BfuAI ACCTGC 1 cut(s) 1476
BfuI GTATCC 1 cut(s) 1722
BisI GCNGC 6 cut(s) 99, 1780, 2548, 2791, 2811, 3127
BlpI GCTNAGC 2 cut(s) 710, 1950
BlsI GCNGC 6 cut(s) 100, 1781, 2549, 2792, 2812, 3128
BmcAI AGTACT 1 cut(s) 2227
Bme18I GGWCC 3 cut(s) 529, 1863, 2677
BmeT110I CYCGRG 1 cut(s) 1056
BmgT120I GGNCC 4 cut(s) 529, 1664, 1863, 2677
BmiI GGNNCC 7 cut(s) 7, 802, 1135, 1470, 1865, 2341, 3043
BmrI ACTGGG 1 cut(s) 731
BmsI GCATC 7 cut(s) 385, 1401, 1447, 1605, 2258, 2409, 2619
BmuI ACTGGG 1 cut(s) 731
BoxI GACNNNNGTC 1 cut(s) 3213
BpiI GAAGAC 7 cut(s) 34, 69, 271, 2024, 2438, 2591, 3056
BplI GAGNNNNNCTC 6 cut(s) 382, 414, 1592, 1624, 3267, 3299
BpmI CTGGAG 4 cut(s) 409, 1122, 1494, 3338
Bpu10I CCTNAGC 1 cut(s) 1813
Bpu1102I GCTNAGC 2 cut(s) 710, 1950
BpuEI CTTGAG 8 cut(s) 532, 545, 1200, 1766, 2537, 2719, 2849, 3245
BpuMI CCSGG 2 cut(s) 1158, 1868
Bsa29I ATCGAT 1 cut(s) 2417
BsaAI YACGTR 2 cut(s) 95, 2845
BsaI GGTCTC 1 cut(s) 2083
BsaJI CCNNGG 7 cut(s) 9, 112, 300, 1660, 1866, 2785, 3083
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 1867, 1897
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 439, 1352, 2846
Bse1I ACTGG 6 cut(s) 726, 1248, 2173, 2233, 2951, 3355
Bse21I CCTNAGG 1 cut(s) 3244
Bse3DI GCAATG 2 cut(s) 500, 1944
BseBI CCWGG 9 cut(s) 202, 388, 694, 1473, 1861, 2378, 2534, 2874, 3084
BseCI ATCGAT 1 cut(s) 2417
BseDI CCNNGG 7 cut(s) 9, 112, 300, 1660, 1866, 2785, 3083
BseLI CCNNNNNNNGG 4 cut(s) 301, 439, 1352, 2846
BseMI GCAATG 2 cut(s) 500, 1944
BseNI ACTGG 6 cut(s) 726, 1248, 2173, 2233, 2951, 3355
BsePI GCGCGC 1 cut(s) 382
BseRI GAGGAG 2 cut(s) 129, 2534
BseXI GCAGC 6 cut(s) 85, 1791, 2534, 2777, 2822, 3138
BsgI GTGCAG 1 cut(s) 3039
Bsh1236I CGCG 2 cut(s) 384, 1015
Bsh1285I CGRYCG 1 cut(s) 2157
BshFI GGCC 4 cut(s) 1604, 1665, 2172, 2917
BshNI GGYRCC 1 cut(s) 2339
BshVI ATCGAT 1 cut(s) 2417
BsiEI CGRYCG 1 cut(s) 2157
BsiHKAI GWGCWC 3 cut(s) 1956, 2937, 3159
BsiHKCI CYCGRG 1 cut(s) 1056
BsiSI CCGG 3 cut(s) 1157, 1868, 2675
BslFI GGGAC 3 cut(s) 1449, 1607, 1756
BslI CCNNNNNNNGG 4 cut(s) 301, 439, 1352, 2846
BsmAI GTCTC 3 cut(s) 2083, 2258, 3287
BsmBI CGTCTC 1 cut(s) 3287
BsmFI GGGAC 3 cut(s) 1449, 1607, 1756
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 4 cut(s) 1604, 1665, 2172, 2917
Bso31I GGTCTC 1 cut(s) 2083
BsoBI CYCGRG 1 cut(s) 1056
Bsp1286I GDGCHC 4 cut(s) 296, 1956, 2937, 3159
Bsp1407I TGTACA 1 cut(s) 1916
Bsp143I GATC 7 cut(s) 5, 1072, 1133, 1468, 1932, 2154, 2353
Bsp1720I GCTNAGC 2 cut(s) 710, 1950
BspACI CCGC 5 cut(s) 615, 1247, 2337, 2374, 3273
BspANI GGCC 4 cut(s) 1604, 1665, 2172, 2917
BspDI ATCGAT 1 cut(s) 2417
BspFNI CGCG 2 cut(s) 384, 1015
BspHI TCATGA 1 cut(s) 1498
BspLI GGNNCC 7 cut(s) 7, 802, 1135, 1470, 1865, 2341, 3043
BspMAI CTGCAG 2 cut(s) 1991, 3128
BspMI ACCTGC 1 cut(s) 1476
BspPI GGATC 6 cut(s) 13, 1080, 1128, 1141, 1463, 1476
BspQI GCTCTTC 2 cut(s) 405, 1028
BspT107I GGYRCC 1 cut(s) 2339
BspTI CTTAAG 1 cut(s) 1979
BspTNI GGTCTC 1 cut(s) 2083
BsrDI GCAATG 2 cut(s) 500, 1944
BsrGI TGTACA 1 cut(s) 1916
BsrI ACTGG 6 cut(s) 726, 1248, 2173, 2233, 2951, 3355
BssECI CCNNGG 7 cut(s) 9, 112, 300, 1660, 1866, 2785, 3083
BssHII GCGCGC 1 cut(s) 382
BssMI GATC 7 cut(s) 5, 1072, 1133, 1468, 1932, 2154, 2353
BssSI CACGAG 1 cut(s) 1731
BssT1I CCWWGG 2 cut(s) 1660, 2785
Bst2BI CACGAG 1 cut(s) 1731
Bst2UI CCWGG 9 cut(s) 202, 388, 694, 1473, 1861, 2378, 2534, 2874, 3084
Bst4CI ACNGT 5 cut(s) 1879, 2491, 2510, 2588, 3212
Bst6I CTCTTC 3 cut(s) 405, 1028, 3260
BstAFI CTTAAG 1 cut(s) 1979
BstAPI GCANNNNNTGC 3 cut(s) 511, 2229, 2981
BstAUI TGTACA 1 cut(s) 1916
BstBAI YACGTR 2 cut(s) 95, 2845
BstC8I GCNNGC 4 cut(s) 259, 384, 2221, 3124
BstDSI CCRYGG 1 cut(s) 9
BstENI CCTNNNNNAGG 2 cut(s) 437, 2844
BstFNI CGCG 2 cut(s) 384, 1015
BstHHI GCGC 3 cut(s) 22, 384, 386
BstKTI GATC 7 cut(s) 8, 1075, 1136, 1471, 1935, 2157, 2356
BstMAI GTCTC 3 cut(s) 2083, 2258, 3287
BstMBI GATC 7 cut(s) 5, 1072, 1133, 1468, 1932, 2154, 2353
BstMCI CGRYCG 1 cut(s) 2157
BstMWI GCNNNNNNNGC 9 cut(s) 511, 961, 1960, 2229, 2626, 2810, 2981, 3163, 3346
BstNI CCWGG 9 cut(s) 202, 388, 694, 1473, 1861, 2378, 2534, 2874, 3084
BstNSI RCATGY 2 cut(s) 1196, 1590
BstPAI GACNNNNGTC 1 cut(s) 3213
BstSFI CTRYAG 3 cut(s) 1987, 2133, 3124
BstSNI TACGTA 1 cut(s) 2845
BstUI CGCG 2 cut(s) 384, 1015
BstV1I GCAGC 6 cut(s) 85, 1791, 2534, 2777, 2822, 3138
BstV2I GAAGAC 7 cut(s) 34, 69, 271, 2024, 2438, 2591, 3056
BstX2I RGATCY 4 cut(s) 5, 1072, 1133, 1468
BstYI RGATCY 4 cut(s) 5, 1072, 1133, 1468
Bsu15I ATCGAT 1 cut(s) 2417
Bsu36I CCTNAGG 1 cut(s) 3244
BsuI GTATCC 1 cut(s) 1722
BsuRI GGCC 4 cut(s) 1604, 1665, 2172, 2917
BsuTUI ATCGAT 1 cut(s) 2417
BtgI CCRYGG 1 cut(s) 9
BtsI GCAGTG 2 cut(s) 2884, 3051
BtsIMutI CAGTG 8 cut(s) 61, 719, 2269, 2496, 2509, 2884, 2944, 3051
BveI ACCTGC 1 cut(s) 1476
Cac8I GCNNGC 4 cut(s) 259, 384, 2221, 3124
CaiI CAGNNNCTG 3 cut(s) 1855, 2471, 2508
CciI TCATGA 1 cut(s) 1498
CfoI GCGC 3 cut(s) 22, 384, 386
Cfr13I GGNCC 4 cut(s) 529, 1664, 1863, 2677
ClaI ATCGAT 1 cut(s) 2417
CseI GACGC 1 cut(s) 1004
Csp6I GTAC 7 cut(s) 305, 1448, 1536, 1917, 2226, 2340, 3195
CspCI CAANNNNNGTGG 2 cut(s) 2245, 2280
CviQI GTAC 7 cut(s) 305, 1448, 1536, 1917, 2226, 2340, 3195
DpnI GATC 7 cut(s) 7, 1074, 1135, 1470, 1934, 2156, 2355
DpnII GATC 7 cut(s) 5, 1072, 1133, 1468, 1932, 2154, 2353
DraI TTTAAA 2 cut(s) 1042, 3238
Eam1104I CTCTTC 3 cut(s) 405, 1028, 3260
EarI CTCTTC 3 cut(s) 405, 1028, 3260
EciI GGCGGA 1 cut(s) 1262
Ecl136II GAGCTC 2 cut(s) 1954, 2935
Eco105I TACGTA 1 cut(s) 2845
Eco130I CCWWGG 2 cut(s) 1660, 2785
Eco147I AGGCCT 2 cut(s) 1604, 2917
Eco24I GRGCYC 3 cut(s) 296, 1956, 2937
Eco31I GGTCTC 1 cut(s) 2083
Eco47I GGWCC 3 cut(s) 529, 1863, 2677
Eco53kI GAGCTC 2 cut(s) 1954, 2935
Eco57I CTGAAG 3 cut(s) 212, 1208, 2615
Eco81I CCTNAGG 1 cut(s) 3244
Eco88I CYCGRG 1 cut(s) 1056
EcoICRI GAGCTC 2 cut(s) 1954, 2935
EcoNI CCTNNNNNAGG 2 cut(s) 437, 2844
EcoO109I RGGNCCY 2 cut(s) 529, 1863
EcoRII CCWGG 9 cut(s) 200, 386, 692, 1471, 1859, 2376, 2532, 2872, 3082
EcoT14I CCWWGG 2 cut(s) 1660, 2785
EcoT22I ATGCAT 4 cut(s) 1177, 1416, 1620, 2977
EcoT38I GRGCYC 3 cut(s) 296, 1956, 2937
ErhI CCWWGG 2 cut(s) 1660, 2785
Esp3I CGTCTC 1 cut(s) 3287
FalI AAGNNNNNCTT 2 cut(s) 2212, 2244
FaqI GGGAC 3 cut(s) 1449, 1607, 1756
FauI CCCGC 1 cut(s) 2381
FblI GTMKAC 2 cut(s) 2440, 3001
Fnu4HI GCNGC 6 cut(s) 99, 1780, 2548, 2791, 2811, 3127
FriOI GRGCYC 3 cut(s) 296, 1956, 2937
Fsp4HI GCNGC 6 cut(s) 99, 1780, 2548, 2791, 2811, 3127
FspBI CTAG 5 cut(s) 533, 939, 1271, 1325, 3327
FspI TGCGCA 1 cut(s) 21
GlaI GCGC 3 cut(s) 21, 383, 385
GluI GCNGC 6 cut(s) 99, 1780, 2548, 2791, 2811, 3127
GsuI CTGGAG 4 cut(s) 409, 1122, 1494, 3338
HaeIII GGCC 4 cut(s) 1604, 1665, 2172, 2917
HapII CCGG 3 cut(s) 1157, 1868, 2675
HgaI GACGC 1 cut(s) 1004
HhaI GCGC 3 cut(s) 22, 384, 386
Hin6I GCGC 3 cut(s) 20, 382, 384
HinP1I GCGC 3 cut(s) 20, 382, 384
HincII GTYRAC 5 cut(s) 207, 793, 1765, 2779, 3002
HindII GTYRAC 5 cut(s) 207, 793, 1765, 2779, 3002
HindIII AAGCTT 2 cut(s) 2276, 2627
HpaI GTTAAC 2 cut(s) 793, 1765
HpaII CCGG 3 cut(s) 1157, 1868, 2675
HphI GGTGA 7 cut(s) 341, 682, 1019, 1939, 3194, 3212, 3274
Hpy166II GTNNAC 7 cut(s) 207, 793, 1765, 2441, 2779, 3002, 3231
Hpy188I TCNGA 8 cut(s) 105, 301, 1347, 1628, 1824, 2166, 2634, 2890
Hpy8I GTNNAC 7 cut(s) 207, 793, 1765, 2441, 2779, 3002, 3231
Hpy99I CGWCG 4 cut(s) 125, 1615, 3003, 3006
HpyCH4III ACNGT 5 cut(s) 1879, 2491, 2510, 2588, 3212
HpyCH4IV ACGT 3 cut(s) 94, 355, 2844
HpyF10VI GCNNNNNNNGC 9 cut(s) 511, 961, 1960, 2229, 2626, 2810, 2981, 3163, 3346
HpySE526I ACGT 3 cut(s) 94, 355, 2844
HspAI GCGC 3 cut(s) 20, 382, 384
KpnI GGTACC 1 cut(s) 2343
KspAI GTTAAC 2 cut(s) 793, 1765
Kzo9I GATC 7 cut(s) 5, 1072, 1133, 1468, 1932, 2154, 2353
LguI GCTCTTC 2 cut(s) 405, 1028
LmnI GCTCC 5 cut(s) 800, 918, 2323, 2932, 2940
Lsp1109I GCAGC 6 cut(s) 85, 1791, 2534, 2777, 2822, 3138
LweI GCATC 7 cut(s) 385, 1401, 1447, 1605, 2258, 2409, 2619
MaeI CTAG 5 cut(s) 533, 939, 1271, 1325, 3327
MaeII ACGT 3 cut(s) 94, 355, 2844
MaeIII GTNAC 5 cut(s) 107, 329, 1879, 2504, 2524
MalI GATC 7 cut(s) 7, 1074, 1135, 1470, 1934, 2156, 2355
MboI GATC 7 cut(s) 5, 1072, 1133, 1468, 1932, 2154, 2353
MfeI CAATTG 3 cut(s) 174, 734, 1442
MflI RGATCY 4 cut(s) 5, 1072, 1133, 1468
MhlI GDGCHC 4 cut(s) 296, 1956, 2937, 3159
MlyI GAGTC 6 cut(s) 126, 539, 933, 1019, 3000, 3285
MmeI TCCRAC 3 cut(s) 496, 2088, 3286
Mph1103I ATGCAT 4 cut(s) 1177, 1416, 1620, 2977
MroXI GAANNNNTTC 1 cut(s) 2031
MslI CAYNNNNRTG 1 cut(s) 1503
MspCI CTTAAG 1 cut(s) 1979
MspI CCGG 3 cut(s) 1157, 1868, 2675
MunI CAATTG 3 cut(s) 174, 734, 1442
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 9 cut(s) 202, 388, 694, 1473, 1861, 2378, 2534, 2874, 3084
MvnI CGCG 2 cut(s) 384, 1015
MwoI GCNNNNNNNGC 9 cut(s) 511, 961, 1960, 2229, 2626, 2810, 2981, 3163, 3346
NciI CCSGG 2 cut(s) 1158, 1868
NdeII GATC 7 cut(s) 5, 1072, 1133, 1468, 1932, 2154, 2353
NlaIV GGNNCC 7 cut(s) 7, 802, 1135, 1470, 1865, 2341, 3043
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 2 cut(s) 329, 2504
NsbI TGCGCA 1 cut(s) 21
NsiI ATGCAT 4 cut(s) 1177, 1416, 1620, 2977
NspI RCATGY 2 cut(s) 1196, 1590
PagI TCATGA 1 cut(s) 1498
PauI GCGCGC 1 cut(s) 382
PceI AGGCCT 2 cut(s) 1604, 2917
PciSI GCTCTTC 2 cut(s) 405, 1028
PcsI WCGNNNNNNNCGW 2 cut(s) 861, 3126
PctI GAATGC 1 cut(s) 248
PdmI GAANNNNTTC 1 cut(s) 2031
PfeI GAWTC 4 cut(s) 2468, 2497, 2823, 2835
PfoI TCCNGGA 3 cut(s) 1156, 1471, 2532
PkrI GCNGC 6 cut(s) 100, 1781, 2549, 2792, 2812, 3128
Ple19I CGATCG 1 cut(s) 2157
PleI GAGTC 6 cut(s) 125, 539, 932, 1018, 3000, 3284
PpsI GAGTC 6 cut(s) 125, 539, 932, 1018, 3000, 3284
Ppu21I YACGTR 2 cut(s) 95, 2845
PpuMI RGGWCCY 2 cut(s) 529, 1863
PshAI GACNNNNGTC 1 cut(s) 3213
PshBI ATTAAT 4 cut(s) 1901, 2067, 2349, 2760
Psp124BI GAGCTC 2 cut(s) 1956, 2937
Psp5II RGGWCCY 2 cut(s) 529, 1863
Psp6I CCWGG 9 cut(s) 200, 386, 692, 1471, 1859, 2376, 2532, 2872, 3082
PspGI CCWGG 9 cut(s) 200, 386, 692, 1471, 1859, 2376, 2532, 2872, 3082
PspN4I GGNNCC 7 cut(s) 7, 802, 1135, 1470, 1865, 2341, 3043
PspPI GGNCC 4 cut(s) 529, 1664, 1863, 2677
PspPPI RGGWCCY 2 cut(s) 529, 1863
PstI CTGCAG 2 cut(s) 1991, 3128
PstNI CAGNNNCTG 3 cut(s) 1855, 2471, 2508
PsuI RGATCY 4 cut(s) 5, 1072, 1133, 1468
PteI GCGCGC 1 cut(s) 382
PvuI CGATCG 1 cut(s) 2157
RsaI GTAC 7 cut(s) 306, 1449, 1537, 1918, 2227, 2341, 3196
RsaNI GTAC 7 cut(s) 305, 1448, 1536, 1917, 2226, 2340, 3195
RseI CAYNNNNRTG 1 cut(s) 1503
SacI GAGCTC 2 cut(s) 1956, 2937
SalI GTCGAC 1 cut(s) 3000
SapI GCTCTTC 2 cut(s) 405, 1028
SatI GCNGC 6 cut(s) 99, 1780, 2548, 2791, 2811, 3127
Sau3AI GATC 7 cut(s) 5, 1072, 1133, 1468, 1932, 2154, 2353
Sau96I GGNCC 4 cut(s) 529, 1664, 1863, 2677
ScaI AGTACT 1 cut(s) 2227
SchI GAGTC 6 cut(s) 126, 539, 933, 1019, 3000, 3285
SduI GDGCHC 4 cut(s) 296, 1956, 2937, 3159
SfaNI GCATC 7 cut(s) 385, 1401, 1447, 1605, 2258, 2409, 2619
SfcI CTRYAG 3 cut(s) 1987, 2133, 3124
SgrDI CGTCGACG 1 cut(s) 3000
SinI GGWCC 3 cut(s) 529, 1863, 2677
SmiMI CAYNNNNRTG 1 cut(s) 1503
SmlI CTYRAG 9 cut(s) 524, 547, 1215, 1745, 1979, 2516, 2698, 2828, 3224
SmoI CTYRAG 9 cut(s) 524, 547, 1215, 1745, 1979, 2516, 2698, 2828, 3224
SnaBI TACGTA 1 cut(s) 2845
SseBI AGGCCT 2 cut(s) 1604, 2917
SsiI CCGC 5 cut(s) 615, 1247, 2337, 2374, 3273
SspI AATATT 2 cut(s) 1030, 1064
SspMI CTAG 5 cut(s) 533, 939, 1271, 1325, 3327
SstI GAGCTC 2 cut(s) 1956, 2937
StuI AGGCCT 2 cut(s) 1604, 2917
StyI CCWWGG 2 cut(s) 1660, 2785
TaaI ACNGT 5 cut(s) 1879, 2491, 2510, 2588, 3212
TaiI ACGT 3 cut(s) 97, 358, 2847
TaqI TCGA 9 cut(s) 372, 864, 1610, 1935, 2153, 2157, 2417, 2905, 3001
TatI WGTACW 2 cut(s) 1916, 2225
TfiI GAWTC 4 cut(s) 2468, 2497, 2823, 2835
TscAI CASTG 8 cut(s) 61, 726, 2269, 2496, 2509, 2884, 2951, 3058
TseFI GTSAC 2 cut(s) 329, 2504
TseI GCWGC 6 cut(s) 98, 1779, 2547, 2790, 2810, 3126
Tsp45I GTSAC 2 cut(s) 329, 2504
TspGWI ACGGA 3 cut(s) 2987, 3054, 3269
TspRI CASTG 8 cut(s) 61, 726, 2269, 2496, 2509, 2884, 2951, 3058
Vha464I CTTAAG 1 cut(s) 1979
VpaK11BI GGWCC 3 cut(s) 529, 1863, 2677
VspI ATTAAT 4 cut(s) 1901, 2067, 2349, 2760
XagI CCTNNNNNAGG 2 cut(s) 437, 2844
XbaI TCTAGA 2 cut(s) 1270, 3326
XceI RCATGY 2 cut(s) 1196, 1590
XcmI CCANNNNNNNNNTGG 2 cut(s) 1781, 2374
XmiI GTMKAC 2 cut(s) 2440, 3001
XmnI GAANNNNTTC 1 cut(s) 2031
XspI CTAG 5 cut(s) 533, 939, 1271, 1325, 3327
ZrmI AGTACT 1 cut(s) 2227
Zsp2I ATGCAT 4 cut(s) 1177, 1416, 1620, 2977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.