Rw1G003890

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
7369565 .. 7381909
12345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003890.1

Sequence Viewer

Length: 3642 bp
ATGGCGTCAACCTCTTCTTGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGGGGGGAAGATACCCGTGGGGGTTTTACCGGTCTTCTGTATGCTGCACTAAATCAGAGAGGAATTCACACCTTTATGGATGACCGAGAGCTTGAGAAAGGAAAATCCATACGGCCTGAACTTTTAGCTGCAATTGAGGAGTCCAGATCTGCGATTGTCATTCTCTCACCCAGATATGCTTGTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAATGAAATGGGCCAACAAGTCCTCCCCGTCTTCTACGGCGTGGTTCCTTCTGTTGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAAAGCAAGCGCCAAGAAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGGGCAGAATAATGAGGACAGTCTAAATGCGTGGAGAGCTGCTTTGACACAGGTCGCCAATCTTTCTGGCTGGGATTTCAACAATTATCGAAATCCAACAAACCTAATTGAGGAAATTGTTAATCATATACTCAAGAAGTCCGTGTATATATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGGTTCCCGCATTGATGATTTATTAAGCAATTACATATGTCCACAGCTTCGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATTTGTCAGGATTTTGACCGATCCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGAATAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAAATTAAAAATATAGAGGATGAATACACAGGAGCTGCTATGATACAAAGGCGGTTGTGTAGAAAAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCACAAGTGGTTTGGTCCAGGGACTAGAATTATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGACGTTGATGCTACGTACAAGGCTAACGGCTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAACGCCCCCCACCAAAAGATTATTTGCATCTGTGCTACGATATTATAGGGTATGCTAAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCCCTGATGAATGGGAAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGACGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAAACTATTGCCAGCTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTGTTATAACTATCTCCAACAACGAACTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCTCAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGGAGAGAAACAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACAAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAAGTTGTCTCAACTTAGTCTTCTTCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACTTGCCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTGTCCCTTAAGATCTCTCCCAAAAAAATTTAAAGCACATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAAAAGCTATGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTAAAAAAATTGTGGAGAGCCCCGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTTCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTCGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGTCATTGAATGTAAGCAACTGCAGAAAACTTGTTTGTCTTCCCAGCACCGTCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAGCATCAGGTAAATATGGAGGAGATGGACTGTTTTGAGGAAACTGATGTGAAGAGTGGGTATACAATAGAAATGTCATCTACCCGTGATCGCATAAAACATGTGAGAGGTTTAATCTTTCATCGATGCAAAGTAGTTTGGGGATCTTTAAATAAGTTTTTGCCTTCTGGATTGGTGCAAAAAGTGAATACACAGCCAATGAGTTTCTGCTTGCCTATATCTCAAAAAGGGAATACAGAGCCAACGAGTTTCGGCTTGCCTATATCTCAAAAAGTGAATACAGAGCCAACGAGTTTCCGCTTGCCTATTTCTGGTCTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACAAATTCGGTTACTTTCCCTCTCTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCTTTCCTTCAGGCATTCGATTGCTTTCTAAACTTGAGAACTTTAACTTGGAGAATTGTAAGAGACTTAAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAATAGATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATGAAGGATGCAATAACATAGCATTTGAAATGCTGAGGACATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCATTAAGTGTGTCTCTACCTGTACATTGGAATAACAGTCAGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCGGTGGATGAGCTTTATACAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTAAATCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGCTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGAAGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTAGGATGTCTCAAGAGGATATATTGGCTGGCACTGGTACCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1213

Amino Acids

138.02

Weight (kDa)

8.21

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 192 2.5e-50 TIR domain
TIR_2 PF13676 14 - 123 1.6e-14 TIR domain
NB-ARC PF00931 218 - 373 9.2e-21 NB-ARC domain
AAA_22 PF13401 220 - 317 5.5e-06 AAA domain
NACHT PF05729 222 - 319 2.1e-07 NACHT domain
WHD_ROQ1 PF23282 439 - 510 1.2e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 608 - 626 4.5e-06 Leucine Rich Repeat
LRR_13 PF23286 699 - 783 1.6e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 710 - 780 6.1e-09 Leucine-rich repeat region
C-JID PF20160 1032 - 1181 9e-21 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1454
AarI CACCTGC 1 cut(s) 1937
Acc36I ACCTGC 1 cut(s) 1937
Acc65I GGTACC 1 cut(s) 3611
AccB1I GGYRCC 2 cut(s) 342, 3611
AccB7I CCANNNNNTGG 2 cut(s) 1624, 3238
AccI GTMKAC 2 cut(s) 2414, 2887
AciI CCGC 5 cut(s) 340, 607, 682, 889, 2650
AclI AACGTT 1 cut(s) 2170
AclWI GGATC 6 cut(s) 664, 677, 741, 939, 1383, 2503
AcsI RAATTY 8 cut(s) 117, 726, 1805, 1879, 2732, 2944, 3260, 3365
AcuI CTGAAG 3 cut(s) 2781, 3323, 3515
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 1052, 2914, 3168, 3219, 3430, 3613
AfiI CCNNNNNNNGG 5 cut(s) 526, 1421, 1624, 2663, 3238
AflII CTTAAG 1 cut(s) 1786
AflIII ACRYGT 1 cut(s) 2452
AgeI ACCGGT 1 cut(s) 83
AhdI GACNNNNNGTC 1 cut(s) 1779
AjiI CACGTC 1 cut(s) 1288
AjnI CCWGG 4 cut(s) 982, 1540, 3097, 3372
AjuI GAANNNNNNNTTGG 2 cut(s) 2819, 2851
Alw21I GWGCWC 2 cut(s) 366, 3231
AlwI GGATC 6 cut(s) 664, 677, 741, 939, 1383, 2503
AlwNI CAGNNNCTG 1 cut(s) 872
Ama87I CYCGRG 3 cut(s) 708, 2066, 2147
AoxI GGCC 5 cut(s) 167, 292, 1732, 2239, 3485
ApeKI GCWGC 4 cut(s) 98, 182, 455, 872
ApoI RAATTY 8 cut(s) 117, 726, 1805, 1879, 2732, 2944, 3260, 3365
AseI ATTAAT 1 cut(s) 2111
AsiGI ACCGGT 1 cut(s) 83
Asp700I GAANNNNTTC 7 cut(s) 601, 796, 813, 1083, 1515, 1609, 3081
Asp718I GGTACC 1 cut(s) 3611
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 4 cut(s) 292, 980, 1720, 3485
AsuC2I CCSGG 1 cut(s) 3489
AsuHPI GGTGA 5 cut(s) 213, 2723, 2767, 3380, 3511
AvaI CYCGRG 3 cut(s) 708, 2066, 2147
AvaII GGWCC 2 cut(s) 980, 1720
BamHI GGATCC 1 cut(s) 669
BanI GGYRCC 2 cut(s) 342, 3611
BanII GRGCYC 2 cut(s) 366, 1937
BauI CACGAG 2 cut(s) 1190, 3411
BbsI GAAGAC 5 cut(s) 80, 304, 1578, 1691, 2276
Bbv12I GWGCWC 2 cut(s) 366, 3231
BbvCI CCTCAGC 1 cut(s) 3038
BbvI GCAGC 4 cut(s) 85, 169, 442, 859
BccI CCATC 8 cut(s) 826, 1301, 2013, 2371, 2884, 2900, 3135, 3240
BceAI ACGGC 4 cut(s) 182, 334, 1078, 2246
BcgI CGANNNNNNTGC 4 cut(s) 2047, 2081, 2180, 2214
BciT130I CCWGG 4 cut(s) 984, 1542, 3099, 3374
BclI TGATCA 1 cut(s) 721
BcnI CCSGG 1 cut(s) 3489
BfaI CTAG 2 cut(s) 990, 2891
BfmI CTRYAG 1 cut(s) 2266
BfoI RGCGCY 1 cut(s) 379
BfrI CTTAAG 1 cut(s) 1786
BfuAI ACCTGC 1 cut(s) 1937
BglII AGATCT 4 cut(s) 200, 1445, 1790, 1972
BisI GCNGC 5 cut(s) 99, 183, 341, 456, 873
BlsI GCNGC 5 cut(s) 100, 184, 342, 457, 874
Bme1390I CCNGG 5 cut(s) 984, 1542, 3099, 3374, 3489
Bme18I GGWCC 2 cut(s) 980, 1720
BmeRI GACNNNNNGTC 1 cut(s) 1779
BmeT110I CYCGRG 3 cut(s) 708, 2066, 2147
BmgBI CACGTC 1 cut(s) 1288
BmgT120I GGNCC 4 cut(s) 292, 980, 1720, 3485
BmiI GGNNCC 5 cut(s) 327, 344, 604, 671, 3613
BmrFI CCNGG 5 cut(s) 984, 1542, 3099, 3374, 3489
BmrI ACTGGG 1 cut(s) 1785
BmsI GCATC 9 cut(s) 1033, 1150, 1470, 2327, 2365, 2468, 2926, 2948, 3002
BmuI ACTGGG 1 cut(s) 1785
BoxI GACNNNNGTC 1 cut(s) 467
BpiI GAAGAC 5 cut(s) 80, 304, 1578, 1691, 2276
BpmI CTGGAG 1 cut(s) 1610
Bpu10I CCTNAGC 2 cut(s) 1170, 3038
BpuEI CTTGAG 8 cut(s) 167, 533, 1518, 1777, 1982, 2843, 3482, 3570
BpuMI CCSGG 1 cut(s) 3489
Bsa29I ATCGAT 1 cut(s) 2476
BsaAI YACGTR 1 cut(s) 1050
BsaBI GATNNNNATC 2 cut(s) 930, 1374
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1569
BsaJI CCNNGG 5 cut(s) 70, 673, 983, 1618, 2014
BsaWI WCCGGW 2 cut(s) 83, 3235
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 5 cut(s) 526, 1421, 1624, 2663, 3238
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 4 cut(s) 1317, 1780, 2242, 3613
Bse3DI GCAATG 1 cut(s) 3010
Bse8I GATNNNNATC 2 cut(s) 930, 1374
BseBI CCWGG 4 cut(s) 984, 1542, 3099, 3374
BseCI ATCGAT 1 cut(s) 2476
BseDI CCNNGG 5 cut(s) 70, 673, 983, 1618, 2014
BseJI GATNNNNATC 2 cut(s) 930, 1374
BseLI CCNNNNNNNGG 5 cut(s) 526, 1421, 1624, 2663, 3238
BseMI GCAATG 1 cut(s) 3010
BseMII CTCAG 3 cut(s) 2684, 3029, 3105
BseNI ACTGG 4 cut(s) 1317, 1780, 2242, 3613
BseRI GAGGAG 4 cut(s) 206, 1521, 1754, 2387
BseXI GCAGC 4 cut(s) 85, 169, 442, 859
BseYI CCCAGC 3 cut(s) 486, 1506, 2288
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 5 cut(s) 169, 294, 1734, 2241, 3487
BshNI GGYRCC 2 cut(s) 342, 3611
BshTI ACCGGT 1 cut(s) 83
BshVI ATCGAT 1 cut(s) 2476
BsiHKAI GWGCWC 2 cut(s) 366, 3231
BsiHKCI CYCGRG 3 cut(s) 708, 2066, 2147
BsiSI CCGG 3 cut(s) 84, 3236, 3488
BslFI GGGAC 2 cut(s) 1000, 1767
BslI CCNNNNNNNGG 5 cut(s) 526, 1421, 1624, 2663, 3238
BsmFI GGGAC 2 cut(s) 1000, 1767
BsmI GAATGC 1 cut(s) 2802
BsnI GGCC 5 cut(s) 169, 294, 1734, 2241, 3487
Bso31I GGTCTC 1 cut(s) 1569
BsoBI CYCGRG 3 cut(s) 708, 2066, 2147
Bsp1286I GDGCHC 3 cut(s) 366, 1937, 3231
Bsp1407I TGTACA 1 cut(s) 3166
Bsp19I CCATGG 1 cut(s) 673
BspACI CCGC 5 cut(s) 340, 607, 682, 889, 2650
BspANI GGCC 5 cut(s) 169, 294, 1734, 2241, 3487
BspCNI CTCAG 3 cut(s) 2685, 3030, 3106
BspDI ATCGAT 1 cut(s) 2476
BspHI TCATGA 1 cut(s) 1567
BspLI GGNNCC 5 cut(s) 327, 344, 604, 671, 3613
BspMAI CTGCAG 1 cut(s) 2270
BspMI ACCTGC 1 cut(s) 1937
BspPI GGATC 6 cut(s) 664, 677, 741, 939, 1383, 2503
BspT107I GGYRCC 2 cut(s) 342, 3611
BspTI CTTAAG 1 cut(s) 1786
BspTNI GGTCTC 1 cut(s) 1569
BsrDI GCAATG 1 cut(s) 3010
BsrFI RCCGGY 1 cut(s) 83
BsrGI TGTACA 1 cut(s) 3166
BsrI ACTGG 4 cut(s) 1317, 1780, 2242, 3613
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 5 cut(s) 70, 673, 983, 1618, 2014
BssNAI GTATAC 1 cut(s) 2415
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1190, 3411
BssT1I CCWWGG 3 cut(s) 673, 1618, 2014
Bst1107I GTATAC 1 cut(s) 2415
Bst2BI CACGAG 2 cut(s) 1190, 3411
Bst2UI CCWGG 4 cut(s) 984, 1542, 3099, 3374
Bst4CI ACNGT 8 cut(s) 437, 1073, 1476, 1527, 2296, 2384, 3182, 3449
Bst6I CTCTTC 3 cut(s) 19, 2399, 3310
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1786
BstAPI GCANNNNNTGC 2 cut(s) 1489, 3538
BstAUI TGTACA 1 cut(s) 3166
BstBAI YACGTR 1 cut(s) 1050
BstDEI CTNAG 7 cut(s) 47, 1170, 1694, 2693, 2768, 3038, 3114
BstDSI CCRYGG 2 cut(s) 70, 673
BstEII GGTNACC 1 cut(s) 2755
BstENI CCTNNNNNAGG 1 cut(s) 524
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 1 cut(s) 378
BstMWI GCNNNNNNNGC 6 cut(s) 452, 684, 1489, 1740, 3206, 3538
BstNI CCWGG 4 cut(s) 984, 1542, 3099, 3374
BstNSI RCATGY 2 cut(s) 682, 2456
BstPAI GACNNNNGTC 1 cut(s) 467
BstPI GGTNACC 1 cut(s) 2755
BstSCI CCNGG 5 cut(s) 982, 1540, 3097, 3372, 3487
BstSFI CTRYAG 1 cut(s) 2266
BstSNI TACGTA 1 cut(s) 1050
BstV1I GCAGC 4 cut(s) 85, 169, 442, 859
BstV2I GAAGAC 5 cut(s) 80, 304, 1578, 1691, 2276
BstX2I RGATCY 6 cut(s) 200, 669, 1445, 1790, 1972, 2495
BstYI RGATCY 6 cut(s) 200, 669, 1445, 1790, 1972, 2495
BstZ17I GTATAC 1 cut(s) 2415
Bsu15I ATCGAT 1 cut(s) 2476
BsuRI GGCC 5 cut(s) 169, 294, 1734, 2241, 3487
BsuTUI ATCGAT 1 cut(s) 2476
BtgI CCRYGG 2 cut(s) 70, 673
BtrI CACGTC 1 cut(s) 1288
BtsIMutI CAGTG 2 cut(s) 2918, 3606
BveI ACCTGC 1 cut(s) 1937
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 1 cut(s) 1567
CfoI GCGC 1 cut(s) 378
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 4 cut(s) 292, 980, 1720, 3485
ClaI ATCGAT 1 cut(s) 2476
Csp6I GTAC 6 cut(s) 1051, 2913, 3167, 3218, 3429, 3612
CspAI ACCGGT 1 cut(s) 83
CviQI GTAC 6 cut(s) 1051, 2913, 3167, 3218, 3429, 3612
DdeI CTNAG 7 cut(s) 47, 1170, 1694, 2693, 2768, 3038, 3114
DraI TTTAAA 2 cut(s) 1810, 2502
DriI GACNNNNNGTC 1 cut(s) 1779
Eam1104I CTCTTC 3 cut(s) 19, 2399, 3310
Eam1105I GACNNNNNGTC 1 cut(s) 1779
EarI CTCTTC 3 cut(s) 19, 2399, 3310
Ecl136II GAGCTC 1 cut(s) 364
Eco105I TACGTA 1 cut(s) 1050
Eco130I CCWWGG 3 cut(s) 673, 1618, 2014
Eco147I AGGCCT 1 cut(s) 1734
Eco24I GRGCYC 2 cut(s) 366, 1937
Eco31I GGTCTC 1 cut(s) 1569
Eco47I GGWCC 2 cut(s) 980, 1720
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 3 cut(s) 2781, 3323, 3515
Eco88I CYCGRG 3 cut(s) 708, 2066, 2147
Eco91I GGTNACC 1 cut(s) 2755
EcoICRI GAGCTC 1 cut(s) 364
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoO65I GGTNACC 1 cut(s) 2755
EcoRI GAATTC 2 cut(s) 117, 3260
EcoRII CCWGG 4 cut(s) 982, 1540, 3097, 3372
EcoT14I CCWWGG 3 cut(s) 673, 1618, 2014
EcoT22I ATGCAT 3 cut(s) 281, 1485, 2941
EcoT38I GRGCYC 2 cut(s) 366, 1937
ErhI CCWWGG 3 cut(s) 673, 1618, 2014
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FaqI GGGAC 2 cut(s) 1000, 1767
FauI CCCGC 2 cut(s) 614, 675
FauNDI CATATG 1 cut(s) 635
FbaI TGATCA 1 cut(s) 721
FblI GTMKAC 2 cut(s) 2414, 2887
Fnu4HI GCNGC 5 cut(s) 99, 183, 341, 456, 873
FriOI GRGCYC 2 cut(s) 366, 1937
Fsp4HI GCNGC 5 cut(s) 99, 183, 341, 456, 873
FspBI CTAG 2 cut(s) 990, 2891
GlaI GCGC 1 cut(s) 377
GluI GCNGC 5 cut(s) 99, 183, 341, 456, 873
GsaI CCCAGC 3 cut(s) 490, 1510, 2292
GsuI CTGGAG 1 cut(s) 1610
HaeII RGCGCY 1 cut(s) 379
HaeIII GGCC 5 cut(s) 169, 294, 1734, 2241, 3487
HapII CCGG 3 cut(s) 84, 3236, 3488
HhaI GCGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HincII GTYRAC 4 cut(s) 9, 580, 939, 1638
HindII GTYRAC 4 cut(s) 9, 580, 939, 1638
HindIII AAGCTT 1 cut(s) 2030
HpaII CCGG 3 cut(s) 84, 3236, 3488
HphI GGTGA 5 cut(s) 213, 2723, 2767, 3380, 3511
Hpy166II GTNNAC 9 cut(s) 9, 580, 641, 939, 1523, 1638, 1720, 2415, 2888
Hpy8I GTNNAC 9 cut(s) 9, 580, 641, 939, 1523, 1638, 1720, 2415, 2888
HpyCH4III ACNGT 8 cut(s) 437, 1073, 1476, 1527, 2296, 2384, 3182, 3449
HpyCH4IV ACGT 6 cut(s) 1038, 1049, 1287, 1549, 2170, 2681
HpyF10VI GCNNNNNNNGC 6 cut(s) 452, 684, 1489, 1740, 3206, 3538
HpyF3I CTNAG 7 cut(s) 47, 1170, 1694, 2693, 2768, 3038, 3114
HpySE526I ACGT 6 cut(s) 1038, 1049, 1287, 1549, 2170, 2681
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 376
KpnI GGTACC 1 cut(s) 3615
Ksp22I TGATCA 1 cut(s) 721
LmnI GCTCC 2 cut(s) 869, 2717
Lsp1109I GCAGC 4 cut(s) 85, 169, 442, 859
LweI GCATC 9 cut(s) 1033, 1150, 1470, 2327, 2365, 2468, 2926, 2948, 3002
MaeI CTAG 2 cut(s) 990, 2891
MaeII ACGT 6 cut(s) 1038, 1049, 1287, 1549, 2170, 2681
MaeIII GTNAC 4 cut(s) 965, 1381, 2738, 2755
MfeI CAATTG 3 cut(s) 186, 2223, 2983
MflI RGATCY 6 cut(s) 200, 669, 1445, 1790, 1972, 2495
MhlI GDGCHC 3 cut(s) 366, 1937, 3231
MlyI GAGTC 4 cut(s) 203, 1755, 2080, 3522
MmeI TCCRAC 3 cut(s) 536, 1488, 2137
Mph1103I ATGCAT 3 cut(s) 281, 1485, 2941
MroXI GAANNNNTTC 7 cut(s) 601, 796, 813, 1083, 1515, 1609, 3081
MslI CAYNNNNRTG 4 cut(s) 128, 596, 1628, 3132
MspCI CTTAAG 1 cut(s) 1786
MspI CCGG 3 cut(s) 84, 3236, 3488
MspR9I CCNGG 5 cut(s) 984, 1542, 3099, 3374, 3489
MunI CAATTG 3 cut(s) 186, 2223, 2983
Mva1269I GAATGC 1 cut(s) 2802
MvaI CCWGG 4 cut(s) 984, 1542, 3099, 3374
MwoI GCNNNNNNNGC 6 cut(s) 452, 684, 1489, 1740, 3206, 3538
NciI CCSGG 1 cut(s) 3489
NcoI CCATGG 1 cut(s) 673
NdeI CATATG 1 cut(s) 635
NlaIV GGNNCC 5 cut(s) 327, 344, 604, 671, 3613
NmeAIII GCCGAG 1 cut(s) 2328
NmuCI GTSAC 3 cut(s) 965, 1381, 2755
NsiI ATGCAT 3 cut(s) 281, 1485, 2941
NspI RCATGY 2 cut(s) 682, 2456
PaeI GCATGC 1 cut(s) 682
PaeR7I CTCGAG 2 cut(s) 708, 2066
PagI TCATGA 1 cut(s) 1567
PaqCI CACCTGC 1 cut(s) 1937
PceI AGGCCT 1 cut(s) 1734
PciI ACATGT 1 cut(s) 2452
PcsI WCGNNNNNNNCGW 1 cut(s) 655
PctI GAATGC 1 cut(s) 2802
PdmI GAANNNNTTC 7 cut(s) 601, 796, 813, 1083, 1515, 1609, 3081
PfeI GAWTC 9 cut(s) 825, 1610, 1957, 2001, 2044, 2096, 2143, 2764, 3060
PflMI CCANNNNNTGG 2 cut(s) 1624, 3238
PfoI TCCNGGA 1 cut(s) 3097
PinAI ACCGGT 1 cut(s) 83
PkrI GCNGC 5 cut(s) 100, 184, 342, 457, 874
PleI GAGTC 4 cut(s) 202, 1755, 2079, 3521
PpsI GAGTC 4 cut(s) 202, 1755, 2079, 3521
Ppu21I YACGTR 1 cut(s) 1050
PscI ACATGT 1 cut(s) 2452
PshAI GACNNNNGTC 1 cut(s) 467
PshBI ATTAAT 1 cut(s) 2111
PsiI TTATAA 1 cut(s) 1454
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 2170
Psp6I CCWGG 4 cut(s) 982, 1540, 3097, 3372
PspEI GGTNACC 1 cut(s) 2755
PspFI CCCAGC 3 cut(s) 486, 1506, 2288
PspGI CCWGG 4 cut(s) 982, 1540, 3097, 3372
PspN4I GGNNCC 5 cut(s) 327, 344, 604, 671, 3613
PspPI GGNCC 4 cut(s) 292, 980, 1720, 3485
PspXI VCTCGAGB 1 cut(s) 708
PstI CTGCAG 1 cut(s) 2270
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 6 cut(s) 200, 669, 1445, 1790, 1972, 2495
RsaI GTAC 6 cut(s) 1052, 2914, 3168, 3219, 3430, 3613
RsaNI GTAC 6 cut(s) 1051, 2913, 3167, 3218, 3429, 3612
RseI CAYNNNNRTG 4 cut(s) 128, 596, 1628, 3132
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 5 cut(s) 99, 183, 341, 456, 873
Sau96I GGNCC 4 cut(s) 292, 980, 1720, 3485
SchI GAGTC 4 cut(s) 203, 1755, 2080, 3522
ScrFI CCNGG 5 cut(s) 984, 1542, 3099, 3374, 3489
SduI GDGCHC 3 cut(s) 366, 1937, 3231
SfaNI GCATC 9 cut(s) 1033, 1150, 1470, 2327, 2365, 2468, 2926, 2948, 3002
SfcI CTRYAG 1 cut(s) 2266
Sfr274I CTCGAG 2 cut(s) 708, 2066
SinI GGWCC 2 cut(s) 980, 1720
SlaI CTCGAG 2 cut(s) 708, 2066
SmiMI CAYNNNNRTG 4 cut(s) 128, 596, 1628, 3132
SnaBI TACGTA 1 cut(s) 1050
SphI GCATGC 1 cut(s) 682
SseBI AGGCCT 1 cut(s) 1734
SsiI CCGC 5 cut(s) 340, 607, 682, 889, 2650
SspI AATATT 2 cut(s) 1335, 3109
SspMI CTAG 2 cut(s) 990, 2891
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 1734
StyD4I CCNGG 5 cut(s) 982, 1540, 3097, 3372, 3487
StyI CCWWGG 3 cut(s) 673, 1618, 2014
TaaI ACNGT 8 cut(s) 437, 1073, 1476, 1527, 2296, 2384, 3182, 3449
TaiI ACGT 6 cut(s) 1041, 1052, 1290, 1552, 2173, 2684
TaqI TCGA 7 cut(s) 505, 709, 1767, 2067, 2190, 2476, 2806
TaqII GACCGA 2 cut(s) 153, 759
TatI WGTACW 3 cut(s) 2912, 3166, 3217
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 9 cut(s) 825, 1610, 1957, 2001, 2044, 2096, 2143, 2764, 3060
TscAI CASTG 2 cut(s) 2925, 3613
TseFI GTSAC 3 cut(s) 965, 1381, 2755
TseI GCWGC 4 cut(s) 98, 182, 455, 872
Tsp45I GTSAC 3 cut(s) 965, 1381, 2755
TspGWI ACGGA 3 cut(s) 547, 1170, 3506
TspRI CASTG 2 cut(s) 2925, 3613
Van91I CCANNNNNTGG 2 cut(s) 1624, 3238
Vha464I CTTAAG 1 cut(s) 1786
VpaK11BI GGWCC 2 cut(s) 980, 1720
VspI ATTAAT 1 cut(s) 2111
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 8 cut(s) 117, 726, 1805, 1879, 2732, 2944, 3260, 3365
XceI RCATGY 2 cut(s) 682, 2456
XcmI CCANNNNNNNNNTGG 3 cut(s) 829, 2012, 3134
XhoI CTCGAG 2 cut(s) 708, 2066
XmiI GTMKAC 2 cut(s) 2414, 2887
XmnI GAANNNNTTC 7 cut(s) 601, 796, 813, 1083, 1515, 1609, 3081
XspI CTAG 2 cut(s) 990, 2891
Zsp2I ATGCAT 3 cut(s) 281, 1485, 2941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.