pycom10g00460

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000105
Physical Location & Seq
Reverse (-)
498853 .. 502852
4000 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00460.2

Sequence Viewer

Length: 3354 bp
ATGGGATCCACGGCCCCCACGCAAACAACCGATTACGATGTCTTCCTCAGTTTCAGAGGCGAAGACACTCGCAGGAGTTTCGCGGACCAACTACATGCTGCTCTGAGCAACCGAGGAGTCGTCGTATTTGGAGATGATGAACTTCAGAGGGGGCGAGGCATTAAACATCATTTGCTCAAGGCAATTGAAAGATCAAAAATTTCAATCGTCATTCTTTCAAGAGACTACGCCAGTTCAACATGGTGCTTGGATGAGCTTCAGCAAATTATGGAATGGAAGAAATGGGCTCGCCAGATAGTTTTTCCTGTCTTCTATGATGTAAGCCCATCCGAGGTACGAAAACAGACAGGTGATTTCGGTGACTCTTTTGCCGAGCACGAAGGACGTTTCAAAGACAATATCGACATAGTGCGCGCCTGGAGGGATGCTCTTCGTCAATTAGCCAATCTGTCTGGATTTGCCCTACACAAGGCCGAGGATGAGTATGAGGCACAATTTATCCAACGAATTGTTCAATGGGTTTTGAATACATTGCAAACTGTGCCACTGCCATCTTATCTTGAGGACCTAGTGGGAATTGACTCAAGAGTAGAGGAACTAAATAGGCTTTTGGACGTCAAATCTAATGATGTTCGCTTCACAGGGATATGCGGAATGGCTGGGATAGGCAAGTCAACTATCGCCAGAGTGATTTTTAACAACATTTCCAATGAATTTGAAATTTTCCCCTGGATTACTTGTGCAACACAGGGTGATCTGCAACTGCAAAAGAAACTTCTTTACGAGGCTTTGAGTCAAGACATAGAGGTTCCAAATGTTAACGAGGGAGCCACAATGATAAAGAACCTTCTATGTAAGAGAAAGGTTCTTCTCATTTTAGACGATGTTTACAACTTACACCAACTGAAATCATTGGCTGGAAACAGAGAATGGTTTGGTTTGGGGAGCAGAGTCATCATTGCGACTAGAGATGAGGAATTGCTAAGAAAGCATGGTGTAGATAGCATATTTGAAGTTAAGGGATTAAGTGAGGACGAGTCGCATCGGCTCTTTAATAATCACGCCTTTAAAAGTGATGAACCCCGAGAAGATTATTTGGATCTGTCTACACAGTTTGTTCAATATGCTCGAGGCCTTCCTTTAACACTCCAGGTTATGGGATCCGCTTTGCTCCACAGAAGTCGGGAAGGGTGGCATGCTGCATTGGAAAAACTGAAGCATGTTCCTGATAAAAATGTTTCTCAAGTACTTGAAATAAGTTATCATGGACTGGCGGACAACGAGAAAAGAATATTTCTAGATATTGCTTGTTTCTTTCGAGGAATGGACAAGGAACGAGTAGTAGAGATACTAGATTGTTTTGGATTTAATGCGATGGTTGGGATGCAAGATCTTGTGGACAGATGTCTCGTAAGTATTTTAAATAACAAAGTGTGGATGCATGACATGATACAAGAAATGGGACAACGAATTGTACGCAGAGACTCTGTTGTGGATCCGGGAGAGCAAAGCAGGTTGTGGCTTCGTGAGGATGTCATTTATGTATTGAGGAATAATACGGGAACAAATGCGGTTGAAGGTATAGCCTTAGACTTGCCTAAATTAGAAGAGGCGTGCTGGGACTTGGAGGCCTTCTCAACGATGCATAATCTCAGATTTCTCCGAATTCATAATTTGCGAATGGCCCAAGGCCCAAAAAAACTTTCTAATGCCTTGAAGTTTCTTGAATGGAGTGGGTATCCTTCAAAACGTCTCCCACGAGAATTTGAATTTGAGCAACTTTGTGAAGTTAACTTGTCCCATAGCAGCGTTGGACAACTTTGGAATAGAACAAAGTGCTTAGGCAATCTGAAATTCGTCAATGTTAGCTACTCCCAGAACCTGACCAGGACCCCGGACTTTACAGTTACTCCAAATCTTCATAGATTAATTCTTGAAGGCTGTACAAACTTGGTGATAATCCACCCATCCATTACTGAGCTCAAAAGGCTTATCTTCTTAAATCTTAAGGACTGCAGAAGTCTTAAATGCCTTCCCAAATGTTTTGAAATGGAAAGTCTTCAAATTCTTATTCTTTCTGGTTGTTCAAAAATTAATAAAGTTCCAGAATTTGTGAGACCTATGGAAAACTTGTTGGAACTTTATTTAGATAAGACTGCTGTAGAAGAATTACCTTCTTCAATCGAATGGCTGACTGGCCTTACTTTATTAGACCTGAGAGATTGCAGAAATCTCAGTTCCCTTGCAAGCAGTACTTGCCAGTTGCGTTCCCTCACAAGTCTCAATCTCAGTGGATGCTCAAAGCTTGAAGAACTGCCAAAAAACTTGGGAAAGATGGTTTGTTTGAAGGAGCTTGATGTGGGCGGTACCTCTATTAATGATCTACCATCCTCCATTTCCCGCCTGGAAAATCTTGAATTGTTATCCCTTTGTGGTTGCAAATCGATGCGAAGAAAGAAGACAAGTCCACTGAGTTTGCTGTTGCCGACCACTGATTCTGGTTTGCTTTCTTTAACAGGACTGAATCTCAGTGACTGTAATCTTGAGGAGGTAACAATCCTGGAAAATCTTGGCTGCTTGTCCTCAATGGTATCGCTTAATCTAAGTAAAAACAGTTTTGTTAGTCTTCCCAAGAGCATCACAGAGCTGTCTAAGCTTCAGATTTTGAATTTGGGTGGTTGTCAGAGGCTTAAAAAATTGCCGGACATTTCAGCAGAATTGAACTTCAGTGTTGAGGGAAAAGGCAGTTATTCACAGGAAAGGCTGTCATCATGTTTCAGTTTTATTAATTGCTTTGAAGTGATTGACGACCAAGGCTGCAACAATGTAGCATTTGCAGCGTTAAGGAGATTCCTTGAGGGAATCCCTTACGTAGGGAATAAATTTGAAGCTATATATCCTGGCAGTGAAATTTCTGAGTGGTTTAGTTATCGAAGTGAGAGGCCTGTTGTAAGCGTGGAGCTCCCTCAACACTGGTATCAGAACGAGTGGATGGGATATGCATTGTGTGCTGTTTTCACTCTCCGTCGACGACTCCCACCTAATCTGCTTGGTAAATGGAACTATGGAACCCATAGCACTGCACATGGTCTTCGTTGCGAAGTGAAGCCTGGGAATTTGGGTGTAGGTGGCTGGTGTCCTTCCTTCGCCTGCAGCGAAGAATTAGGGCAAATTGAGAAAGAGCACCTTTGGCTATCCTTTATATCTGGTGCATACTTTGGTACAATATGGCAAGACAGTTGTCATCACCTTGAGTTTACATTTAAAACCTTAGGCCATGGTTTGGAGGTGAAGAGGTGTGGAGTCCGTCTCATCTACAAGAGAGATTTGGTTGGTTTAAAGCAACCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1118

Amino Acids

126.89

Weight (kDa)

6.34

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 618
Acc36I ACCTGC 1 cut(s) 1503
Acc65I GGTACC 1 cut(s) 2366
AccB1I GGYRCC 1 cut(s) 2366
AccB7I CCANNNNNTGG 2 cut(s) 1156, 3283
AccI GTMKAC 2 cut(s) 1106, 3028
AccII CGCG 2 cut(s) 83, 414
AciI CCGC 7 cut(s) 83, 651, 1164, 1274, 1571, 2364, 2401
AclWI GGATC 6 cut(s) 13, 1107, 1155, 1168, 1490, 1503
AcuI CTGAAG 5 cut(s) 128, 242, 1235, 2642, 2710
AcyI GRCGYC 1 cut(s) 615
AdeI CACNNNGTG 1 cut(s) 752
AfaI GTAC 7 cut(s) 336, 1248, 1476, 1945, 2254, 2368, 3223
AfiI CCNNNNNNNGG 5 cut(s) 331, 469, 1156, 2873, 3283
AflII CTTAAG 1 cut(s) 2006
AjnI CCWGG 8 cut(s) 416, 728, 1149, 1886, 2403, 2559, 2899, 3109
AjuI GAANNNNNNNTTGG 4 cut(s) 495, 527, 1687, 1719
AluBI AGCT 9 cut(s) 256, 1869, 1981, 2305, 2353, 2647, 2656, 2891, 2962
AluI AGCT 9 cut(s) 256, 1869, 1981, 2305, 2353, 2647, 2656, 2891, 2962
Alw21I GWGCWC 4 cut(s) 378, 1983, 2964, 3186
Alw26I GTCTC 7 cut(s) 216, 1412, 1476, 1757, 2110, 2285, 3314
AlwI GGATC 6 cut(s) 13, 1107, 1155, 1168, 1490, 1503
AlwNI CAGNNNCTG 2 cut(s) 1882, 2535
Ama87I CYCGRG 2 cut(s) 1083, 1128
AoxI GGCC 9 cut(s) 12, 471, 1132, 1629, 1683, 1690, 2197, 2942, 3274
ApeKI GCWGC 7 cut(s) 98, 1199, 1806, 2574, 2817, 2837, 3153
AseI ATTAAT 4 cut(s) 1928, 2094, 2376, 2787
Asp700I GAANNNNTTC 1 cut(s) 2058
Asp718I GGTACC 1 cut(s) 2366
AspLEI GCGC 2 cut(s) 414, 416
AspS9I GGNCC 6 cut(s) 13, 85, 565, 1684, 1691, 1890
AsuC2I CCSGG 2 cut(s) 1500, 1895
AsuHPI GGTGA 6 cut(s) 362, 371, 764, 1966, 3239, 3301
AvaI CYCGRG 2 cut(s) 1083, 1128
AvaII GGWCC 3 cut(s) 85, 565, 1890
AxyI CCTNAGG 1 cut(s) 3271
BaeI ACNNNNGTAYC 2 cut(s) 2960, 2993
BamHI GGATCC 3 cut(s) 5, 1160, 1495
BanI GGYRCC 1 cut(s) 2366
BanII GRGCYC 3 cut(s) 289, 1983, 2964
BauI CACGAG 1 cut(s) 1758
BbsI GAAGAC 7 cut(s) 34, 69, 301, 2051, 2465, 2618, 3083
Bbv12I GWGCWC 4 cut(s) 378, 1983, 2964, 3186
BbvI GCAGC 7 cut(s) 85, 1186, 1818, 2561, 2804, 2849, 3165
BccI CCATC 7 cut(s) 334, 559, 1369, 1975, 2329, 2395, 2986
BceAI ACGGC 1 cut(s) 27
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 8 cut(s) 418, 730, 1151, 1888, 2405, 2561, 2901, 3111
BciVI GTATCC 1 cut(s) 1749
BcnI CCSGG 2 cut(s) 1500, 1895
BcoDI GTCTC 7 cut(s) 216, 1412, 1476, 1757, 2110, 2285, 3314
BfaI CTAG 4 cut(s) 569, 966, 1298, 1352
BfmI CTRYAG 3 cut(s) 2014, 2160, 3151
BfrI CTTAAG 1 cut(s) 2006
BfuAI ACCTGC 1 cut(s) 1503
BfuI GTATCC 1 cut(s) 1749
BglII AGATCT 1 cut(s) 1390
BisI GCNGC 7 cut(s) 99, 1200, 1807, 2575, 2818, 2838, 3154
BlsI GCNGC 7 cut(s) 100, 1201, 1808, 2576, 2819, 2839, 3155
BmcAI AGTACT 2 cut(s) 1248, 2254
Bme18I GGWCC 3 cut(s) 85, 565, 1890
BmeT110I CYCGRG 2 cut(s) 1083, 1128
BmgT120I GGNCC 6 cut(s) 13, 85, 565, 1684, 1691, 1890
BmiI GGNNCC 9 cut(s) 7, 15, 810, 829, 1162, 1497, 1892, 2368, 3070
BmsI GCATC 8 cut(s) 415, 1051, 1374, 1428, 1632, 2285, 2436, 2646
BoxI GACNNNNGTC 2 cut(s) 1404, 3240
BpiI GAAGAC 7 cut(s) 34, 69, 301, 2051, 2465, 2618, 3083
BplI GAGNNNNNCTC 6 cut(s) 412, 444, 1619, 1651, 3294, 3326
BpmI CTGGAG 2 cut(s) 439, 1133
Bpu10I CCTNAGC 1 cut(s) 1840
BpuEI CTTGAG 7 cut(s) 161, 568, 581, 1227, 2564, 2876, 3272
BpuMI CCSGG 2 cut(s) 1500, 1895
Bsa29I ATCGAT 1 cut(s) 2444
BsaAI YACGTR 1 cut(s) 2872
BsaHI GRCGYC 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 2110
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 1894, 1924
Bsc4I CCNNNNNNNGG 5 cut(s) 331, 469, 1156, 2873, 3283
Bse1I ACTGG 5 cut(s) 231, 1275, 2200, 2260, 2978
Bse21I CCTNAGG 1 cut(s) 3271
Bse3DI GCAATG 2 cut(s) 530, 957
BseBI CCWGG 8 cut(s) 418, 730, 1151, 1888, 2405, 2561, 2901, 3111
BseCI ATCGAT 1 cut(s) 2444
BseLI CCNNNNNNNGG 5 cut(s) 331, 469, 1156, 2873, 3283
BseMI GCAATG 2 cut(s) 530, 957
BseNI ACTGG 5 cut(s) 231, 1275, 2200, 2260, 2978
BsePI GCGCGC 1 cut(s) 412
BseRI GAGGAG 2 cut(s) 129, 2561
BseXI GCAGC 7 cut(s) 85, 1186, 1818, 2561, 2804, 2849, 3165
BseYI CCCAGC 2 cut(s) 659, 1617
BsgI GTGCAG 1 cut(s) 3066
Bsh1236I CGCG 2 cut(s) 83, 414
BshFI GGCC 9 cut(s) 14, 473, 1134, 1631, 1685, 1692, 2199, 2944, 3276
BshNI GGYRCC 1 cut(s) 2366
BshVI ATCGAT 1 cut(s) 2444
BsiHKAI GWGCWC 4 cut(s) 378, 1983, 2964, 3186
BsiHKCI CYCGRG 2 cut(s) 1083, 1128
BsiSI CCGG 3 cut(s) 1499, 1895, 2702
BslFI GGGAC 3 cut(s) 1476, 1634, 1783
BslI CCNNNNNNNGG 5 cut(s) 331, 469, 1156, 2873, 3283
BsmAI GTCTC 7 cut(s) 216, 1412, 1476, 1757, 2110, 2285, 3314
BsmBI CGTCTC 2 cut(s) 1757, 3314
BsmFI GGGAC 3 cut(s) 1476, 1634, 1783
BsnI GGCC 9 cut(s) 14, 473, 1134, 1631, 1685, 1692, 2199, 2944, 3276
Bso31I GGTCTC 1 cut(s) 2110
BsoBI CYCGRG 2 cut(s) 1083, 1128
Bsp1286I GDGCHC 5 cut(s) 289, 378, 1983, 2964, 3186
Bsp1407I TGTACA 1 cut(s) 1943
Bsp143I GATC 8 cut(s) 5, 191, 754, 1099, 1160, 1390, 1495, 2380
Bsp19I CCATGG 1 cut(s) 3277
BspACI CCGC 7 cut(s) 83, 651, 1164, 1274, 1571, 2364, 2401
BspANI GGCC 9 cut(s) 14, 473, 1134, 1631, 1685, 1692, 2199, 2944, 3276
BspDI ATCGAT 1 cut(s) 2444
BspFNI CGCG 2 cut(s) 83, 414
BspLI GGNNCC 9 cut(s) 7, 15, 810, 829, 1162, 1497, 1892, 2368, 3070
BspMAI CTGCAG 2 cut(s) 2018, 3155
BspMI ACCTGC 1 cut(s) 1503
BspPI GGATC 6 cut(s) 13, 1107, 1155, 1168, 1490, 1503
BspQI GCTCTTC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 2366
BspTI CTTAAG 1 cut(s) 2006
BspTNI GGTCTC 1 cut(s) 2110
BsrDI GCAATG 2 cut(s) 530, 957
BsrGI TGTACA 1 cut(s) 1943
BsrI ACTGG 5 cut(s) 231, 1275, 2200, 2260, 2978
BssHII GCGCGC 1 cut(s) 412
BssMI GATC 8 cut(s) 5, 191, 754, 1099, 1160, 1390, 1495, 2380
BssNI GRCGYC 1 cut(s) 615
BssSI CACGAG 1 cut(s) 1758
BssT1I CCWWGG 3 cut(s) 1687, 2812, 3277
Bst2BI CACGAG 1 cut(s) 1758
Bst2UI CCWGG 8 cut(s) 418, 730, 1151, 1888, 2405, 2561, 2901, 3111
Bst4CI ACNGT 6 cut(s) 541, 1113, 1906, 2537, 2615, 3239
Bst6I CTCTTC 3 cut(s) 435, 1602, 3287
BstACI GRCGYC 1 cut(s) 615
BstAFI CTTAAG 1 cut(s) 2006
BstAPI GCANNNNNTGC 3 cut(s) 541, 2256, 3008
BstAUI TGTACA 1 cut(s) 1943
BstBAI YACGTR 1 cut(s) 2872
BstC8I GCNNGC 6 cut(s) 289, 414, 1197, 1615, 2248, 3151
BstDSI CCRYGG 2 cut(s) 9, 3277
BstENI CCTNNNNNAGG 2 cut(s) 467, 2871
BstFNI CGCG 2 cut(s) 83, 414
BstHHI GCGC 2 cut(s) 414, 416
BstKTI GATC 8 cut(s) 8, 194, 757, 1102, 1163, 1393, 1498, 2383
BstMAI GTCTC 7 cut(s) 216, 1412, 1476, 1757, 2110, 2285, 3314
BstMBI GATC 8 cut(s) 5, 191, 754, 1099, 1160, 1390, 1495, 2380
BstMWI GCNNNNNNNGC 8 cut(s) 541, 988, 1987, 2256, 2653, 2837, 3008, 3190
BstNI CCWGG 8 cut(s) 418, 730, 1151, 1888, 2405, 2561, 2901, 3111
BstNSI RCATGY 3 cut(s) 98, 1199, 1223
BstPAI GACNNNNGTC 2 cut(s) 1404, 3240
BstSFI CTRYAG 3 cut(s) 2014, 2160, 3151
BstSNI TACGTA 1 cut(s) 2872
BstUI CGCG 2 cut(s) 83, 414
BstV1I GCAGC 7 cut(s) 85, 1186, 1818, 2561, 2804, 2849, 3165
BstV2I GAAGAC 7 cut(s) 34, 69, 301, 2051, 2465, 2618, 3083
BstX2I RGATCY 5 cut(s) 5, 1099, 1160, 1390, 1495
BstYI RGATCY 5 cut(s) 5, 1099, 1160, 1390, 1495
Bsu15I ATCGAT 1 cut(s) 2444
Bsu36I CCTNAGG 1 cut(s) 3271
BsuI GTATCC 1 cut(s) 1749
BsuRI GGCC 9 cut(s) 14, 473, 1134, 1631, 1685, 1692, 2199, 2944, 3276
BsuTUI ATCGAT 1 cut(s) 2444
BtgI CCRYGG 2 cut(s) 9, 3277
BtgZI GCGATG 1 cut(s) 1388
BtsI GCAGTG 3 cut(s) 545, 2911, 3078
BtsIMutI CAGTG 9 cut(s) 545, 2296, 2468, 2490, 2536, 2734, 2911, 2971, 3078
BveI ACCTGC 1 cut(s) 1503
Cac8I GCNNGC 6 cut(s) 289, 414, 1197, 1615, 2248, 3151
CaiI CAGNNNCTG 2 cut(s) 1882, 2535
CfoI GCGC 2 cut(s) 414, 416
Cfr13I GGNCC 6 cut(s) 13, 85, 565, 1684, 1691, 1890
ClaI ATCGAT 1 cut(s) 2444
Csp6I GTAC 7 cut(s) 335, 1247, 1475, 1944, 2253, 2367, 3222
CspCI CAANNNNNGTGG 4 cut(s) 2272, 2307, 2457, 2492
CviQI GTAC 7 cut(s) 335, 1247, 1475, 1944, 2253, 2367, 3222
DpnI GATC 8 cut(s) 7, 193, 756, 1101, 1162, 1392, 1497, 2382
DpnII GATC 8 cut(s) 5, 191, 754, 1099, 1160, 1390, 1495, 2380
DraI TTTAAA 4 cut(s) 1069, 1422, 3265, 3339
DraIII CACNNNGTG 1 cut(s) 752
Eam1104I CTCTTC 3 cut(s) 435, 1602, 3287
EarI CTCTTC 3 cut(s) 435, 1602, 3287
EciI GGCGGA 1 cut(s) 1289
Ecl136II GAGCTC 2 cut(s) 1981, 2962
Eco105I TACGTA 1 cut(s) 2872
Eco130I CCWWGG 3 cut(s) 1687, 2812, 3277
Eco147I AGGCCT 3 cut(s) 1134, 1631, 2944
Eco24I GRGCYC 3 cut(s) 289, 1983, 2964
Eco31I GGTCTC 1 cut(s) 2110
Eco47I GGWCC 3 cut(s) 85, 565, 1890
Eco53kI GAGCTC 2 cut(s) 1981, 2962
Eco57I CTGAAG 5 cut(s) 128, 242, 1235, 2642, 2710
Eco81I CCTNAGG 1 cut(s) 3271
Eco88I CYCGRG 2 cut(s) 1083, 1128
EcoICRI GAGCTC 2 cut(s) 1981, 2962
EcoNI CCTNNNNNAGG 2 cut(s) 467, 2871
EcoO109I RGGNCCY 2 cut(s) 565, 1890
EcoRI GAATTC 1 cut(s) 1665
EcoRII CCWGG 8 cut(s) 416, 728, 1149, 1886, 2403, 2559, 2899, 3109
EcoT14I CCWWGG 3 cut(s) 1687, 2812, 3277
EcoT22I ATGCAT 3 cut(s) 1443, 1647, 3004
EcoT38I GRGCYC 3 cut(s) 289, 1983, 2964
ErhI CCWWGG 3 cut(s) 1687, 2812, 3277
Esp3I CGTCTC 2 cut(s) 1757, 3314
FalI AAGNNNNNCTT 4 cut(s) 2239, 2271, 3171, 3203
FaqI GGGAC 3 cut(s) 1476, 1634, 1783
FauI CCCGC 1 cut(s) 2408
FblI GTMKAC 2 cut(s) 1106, 3028
Fnu4HI GCNGC 7 cut(s) 99, 1200, 1807, 2575, 2818, 2838, 3154
FriOI GRGCYC 3 cut(s) 289, 1983, 2964
Fsp4HI GCNGC 7 cut(s) 99, 1200, 1807, 2575, 2818, 2838, 3154
FspBI CTAG 4 cut(s) 569, 966, 1298, 1352
GlaI GCGC 2 cut(s) 413, 415
GluI GCNGC 7 cut(s) 99, 1200, 1807, 2575, 2818, 2838, 3154
GsaI CCCAGC 2 cut(s) 663, 1621
GsuI CTGGAG 2 cut(s) 439, 1133
HaeIII GGCC 9 cut(s) 14, 473, 1134, 1631, 1685, 1692, 2199, 2944, 3276
HapII CCGG 3 cut(s) 1499, 1895, 2702
HhaI GCGC 2 cut(s) 414, 416
Hin1I GRCGYC 1 cut(s) 615
Hin6I GCGC 2 cut(s) 412, 414
HinP1I GCGC 2 cut(s) 412, 414
HincII GTYRAC 4 cut(s) 675, 820, 1792, 3029
HindII GTYRAC 4 cut(s) 675, 820, 1792, 3029
HindIII AAGCTT 2 cut(s) 2303, 2654
HpaI GTTAAC 2 cut(s) 820, 1792
HpaII CCGG 3 cut(s) 1499, 1895, 2702
HphI GGTGA 6 cut(s) 362, 371, 764, 1966, 3239, 3301
Hpy166II GTNNAC 9 cut(s) 675, 820, 889, 1107, 1399, 1792, 2468, 3029, 3258
Hpy8I GTNNAC 9 cut(s) 675, 820, 889, 1107, 1399, 1792, 2468, 3029, 3258
Hpy99I CGWCG 3 cut(s) 125, 3030, 3033
HpyCH4III ACNGT 6 cut(s) 541, 1113, 1906, 2537, 2615, 3239
HpyCH4IV ACGT 4 cut(s) 385, 615, 1750, 2871
HpyF10VI GCNNNNNNNGC 8 cut(s) 541, 988, 1987, 2256, 2653, 2837, 3008, 3190
HpySE526I ACGT 4 cut(s) 385, 615, 1750, 2871
Hsp92I GRCGYC 1 cut(s) 615
HspAI GCGC 2 cut(s) 412, 414
KpnI GGTACC 1 cut(s) 2370
KspAI GTTAAC 2 cut(s) 820, 1792
Kzo9I GATC 8 cut(s) 5, 191, 754, 1099, 1160, 1390, 1495, 2380
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 6 cut(s) 827, 945, 1176, 2350, 2959, 2967
Lsp1109I GCAGC 7 cut(s) 85, 1186, 1818, 2561, 2804, 2849, 3165
LweI GCATC 8 cut(s) 415, 1051, 1374, 1428, 1632, 2285, 2436, 2646
MaeI CTAG 4 cut(s) 569, 966, 1298, 1352
MaeII ACGT 4 cut(s) 385, 615, 1750, 2871
MaeIII GTNAC 4 cut(s) 359, 1906, 2531, 2551
MalI GATC 8 cut(s) 7, 193, 756, 1101, 1162, 1392, 1497, 2382
MboI GATC 8 cut(s) 5, 191, 754, 1099, 1160, 1390, 1495, 2380
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 5 cut(s) 5, 1099, 1160, 1390, 1495
MhlI GDGCHC 5 cut(s) 289, 378, 1983, 2964, 3186
MlyI GAGTC 9 cut(s) 126, 356, 575, 802, 960, 1046, 1478, 3027, 3312
MmeI TCCRAC 3 cut(s) 526, 1792, 2115
Mph1103I ATGCAT 3 cut(s) 1443, 1647, 3004
MroXI GAANNNNTTC 1 cut(s) 2058
MspCI CTTAAG 1 cut(s) 2006
MspI CCGG 3 cut(s) 1499, 1895, 2702
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 8 cut(s) 418, 730, 1151, 1888, 2405, 2561, 2901, 3111
MvnI CGCG 2 cut(s) 83, 414
MwoI GCNNNNNNNGC 8 cut(s) 541, 988, 1987, 2256, 2653, 2837, 3008, 3190
NciI CCSGG 2 cut(s) 1500, 1895
NcoI CCATGG 1 cut(s) 3277
NdeII GATC 8 cut(s) 5, 191, 754, 1099, 1160, 1390, 1495, 2380
NlaIV GGNNCC 9 cut(s) 7, 15, 810, 829, 1162, 1497, 1892, 2368, 3070
NmeAIII GCCGAG 2 cut(s) 397, 499
NmuCI GTSAC 2 cut(s) 359, 2531
NsiI ATGCAT 3 cut(s) 1443, 1647, 3004
NspI RCATGY 3 cut(s) 98, 1199, 1223
PaeI GCATGC 1 cut(s) 1199
PaeR7I CTCGAG 1 cut(s) 1128
PauI GCGCGC 1 cut(s) 412
PceI AGGCCT 3 cut(s) 1134, 1631, 2944
PciSI GCTCTTC 1 cut(s) 435
PcsI WCGNNNNNNNCGW 2 cut(s) 1756, 3153
PdmI GAANNNNTTC 1 cut(s) 2058
PfeI GAWTC 4 cut(s) 2495, 2524, 2850, 2862
PflMI CCANNNNNTGG 2 cut(s) 1156, 3283
PfoI TCCNGGA 2 cut(s) 1498, 2559
PkrI GCNGC 7 cut(s) 100, 1201, 1808, 2576, 2819, 2839, 3155
PleI GAGTC 9 cut(s) 125, 356, 575, 801, 959, 1045, 1478, 3027, 3311
PpsI GAGTC 9 cut(s) 125, 356, 575, 801, 959, 1045, 1478, 3027, 3311
Ppu21I YACGTR 1 cut(s) 2872
PpuMI RGGWCCY 2 cut(s) 565, 1890
PshAI GACNNNNGTC 2 cut(s) 1404, 3240
PshBI ATTAAT 4 cut(s) 1928, 2094, 2376, 2787
Psp124BI GAGCTC 2 cut(s) 1983, 2964
Psp5II RGGWCCY 2 cut(s) 565, 1890
Psp6I CCWGG 8 cut(s) 416, 728, 1149, 1886, 2403, 2559, 2899, 3109
PspFI CCCAGC 2 cut(s) 659, 1617
PspGI CCWGG 8 cut(s) 416, 728, 1149, 1886, 2403, 2559, 2899, 3109
PspN4I GGNNCC 9 cut(s) 7, 15, 810, 829, 1162, 1497, 1892, 2368, 3070
PspPI GGNCC 6 cut(s) 13, 85, 565, 1684, 1691, 1890
PspPPI RGGWCCY 2 cut(s) 565, 1890
PspXI VCTCGAGB 1 cut(s) 1128
PstI CTGCAG 2 cut(s) 2018, 3155
PstNI CAGNNNCTG 2 cut(s) 1882, 2535
PsuI RGATCY 5 cut(s) 5, 1099, 1160, 1390, 1495
PteI GCGCGC 1 cut(s) 412
RsaI GTAC 7 cut(s) 336, 1248, 1476, 1945, 2254, 2368, 3223
RsaNI GTAC 7 cut(s) 335, 1247, 1475, 1944, 2253, 2367, 3222
SacI GAGCTC 2 cut(s) 1983, 2964
SalI GTCGAC 1 cut(s) 3027
SapI GCTCTTC 1 cut(s) 435
SatI GCNGC 7 cut(s) 99, 1200, 1807, 2575, 2818, 2838, 3154
Sau3AI GATC 8 cut(s) 5, 191, 754, 1099, 1160, 1390, 1495, 2380
Sau96I GGNCC 6 cut(s) 13, 85, 565, 1684, 1691, 1890
ScaI AGTACT 2 cut(s) 1248, 2254
SchI GAGTC 9 cut(s) 126, 356, 575, 802, 960, 1046, 1478, 3027, 3312
SduI GDGCHC 5 cut(s) 289, 378, 1983, 2964, 3186
SfaNI GCATC 8 cut(s) 415, 1051, 1374, 1428, 1632, 2285, 2436, 2646
SfcI CTRYAG 3 cut(s) 2014, 2160, 3151
Sfr274I CTCGAG 1 cut(s) 1128
SgrDI CGTCGACG 1 cut(s) 3027
SinI GGWCC 3 cut(s) 85, 565, 1890
SlaI CTCGAG 1 cut(s) 1128
SmlI CTYRAG 9 cut(s) 176, 560, 583, 1128, 1242, 2006, 2543, 2855, 3251
SmoI CTYRAG 9 cut(s) 176, 560, 583, 1128, 1242, 2006, 2543, 2855, 3251
SnaBI TACGTA 1 cut(s) 2872
SphI GCATGC 1 cut(s) 1199
SseBI AGGCCT 3 cut(s) 1134, 1631, 2944
SsiI CCGC 7 cut(s) 83, 651, 1164, 1274, 1571, 2364, 2401
SspI AATATT 1 cut(s) 1293
SspMI CTAG 4 cut(s) 569, 966, 1298, 1352
SstI GAGCTC 2 cut(s) 1983, 2964
StuI AGGCCT 3 cut(s) 1134, 1631, 2944
StyI CCWWGG 3 cut(s) 1687, 2812, 3277
TaaI ACNGT 6 cut(s) 541, 1113, 1906, 2537, 2615, 3239
TaiI ACGT 4 cut(s) 388, 618, 1753, 2874
TaqI TCGA 7 cut(s) 402, 1129, 1318, 2184, 2444, 2932, 3028
TatI WGTACW 3 cut(s) 1246, 1943, 2252
TfiI GAWTC 4 cut(s) 2495, 2524, 2850, 2862
TscAI CASTG 9 cut(s) 552, 2296, 2475, 2497, 2536, 2734, 2911, 2978, 3085
TseFI GTSAC 2 cut(s) 359, 2531
TseI GCWGC 7 cut(s) 98, 1199, 1806, 2574, 2817, 2837, 3153
Tsp45I GTSAC 2 cut(s) 359, 2531
TspDTI ATGAA 5 cut(s) 153, 726, 1092, 1658, 1910
TspGWI ACGGA 2 cut(s) 3014, 3296
TspRI CASTG 9 cut(s) 552, 2296, 2475, 2497, 2536, 2734, 2911, 2978, 3085
Van91I CCANNNNNTGG 2 cut(s) 1156, 3283
Vha464I CTTAAG 1 cut(s) 2006
VpaK11BI GGWCC 3 cut(s) 85, 565, 1890
VspI ATTAAT 4 cut(s) 1928, 2094, 2376, 2787
XagI CCTNNNNNAGG 2 cut(s) 467, 2871
XbaI TCTAGA 1 cut(s) 1297
XceI RCATGY 3 cut(s) 98, 1199, 1223
XcmI CCANNNNNNNNNTGG 2 cut(s) 1808, 2401
XhoI CTCGAG 1 cut(s) 1128
XmiI GTMKAC 2 cut(s) 1106, 3028
XmnI GAANNNNTTC 1 cut(s) 2058
XspI CTAG 4 cut(s) 569, 966, 1298, 1352
ZraI GACGTC 1 cut(s) 616
ZrmI AGTACT 2 cut(s) 1248, 2254
Zsp2I ATGCAT 3 cut(s) 1443, 1647, 3004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.