Rroxscaffold_4G00326440

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Reverse (-)
58491119 .. 58495124
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326440.1

Sequence Viewer

Length: 2688 bp
ATGTACACAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCTGGGAGTAGAATTATCATAACCACCACAGATATTCAGTTATTAAAGGCGCATGATGTTGATGCTCTATACAAGGCTAATGTGTTAAACTATGCTGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAGATGTCCCCCACCAGAAGATTATTTGCATCTGTGCAACCATATTTTAGAGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCCTTTCTATCTGGTAGAGGGGCTGATAAATGGGCAAGTGCAATAGATAGGCTAAGGAGCACACCAGATAAACGCATTATTGACGTGCTTCGGATTAGTTTTGATGACCTGCATAAAAAAGAGAGAGAAATATTCCTACATATTGCATGCTTTTACAAGGGAAAGGATAAGGATCGTGTGACACAAATACTAGAATATTGCCAGCTAGAACCTGTCATTGGTTTAGATGTTCTTGTTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTCAATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTGATGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAGACATAAGGGAACAGATTCAATCCAAGGCATCGTAATGGAGTTGACTAAATTACAAGTGACTCATTGGAATCCAGAAGCCTTTTCAAATTTATCTCAACTTATTCTTCTCCATATTCATAATGTGGACCTTCCTGAAGGCCTCACTTGTCTTCCAAATTCCTTGAGACTCCTGGAATGGACTGGGTATCCCTTAAGATCTCTCCCACAAAACTTTGAAGCAGATGATCTCATTGAACTTAACCTGTGCCAGAGCTACATTGCATATCTTTGGAACGGAACAAAGAATTTTGACAGGTTGAAGTTCATCAAACTCTGCCACTCTCAAAACATTGTGGAGACCCCAGACCTCACAGGCGTTCGGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATAAATCCCTTGGATTTCTCAAAAATCTTATTGTCGTGAATCTTAAAGACTGCAAAAGTCTCAAGACTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAGTTCTTTCTAACTGCTCAAAAATTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGTGTCAATTGAAAGGTTGAGTGGCCTGGTGTCATTGAATCTGAGCAACTGCAGAAATCTTGCTCATCTTCCGAGTACCATCAATAAATTGAAGTCTATTAGAAATCTTAATCTTTCTGGATGCTTGAAACTTGGCAAACATCAAGTAAATGTGGGGGAGATGGACTGTTTTGTGGAAAATGATGTGAATAGTGGGTCTGCAGTAGAAATGTCATCTACCCATGATCTCATAAAATATGTGAGAGGTTCAGTCTTTCGTGGATGCGAAGTAGTTTGGGGATCTGTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAATGAGTTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGATTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTTCTAGCATTAGATTGCTTTCTAAGCTTGAGAACTTCAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAAGGCAGATGGTTGTACTTCACTGAAATACCTGTTTGATGCATCAAATTTGAACGGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCATATGGTAATTCCAGGAAGTAATATTCCTGAGTGGTTCAGCCATCGGAGTGTTGGGTGTTCATTAAGTATGGGACCTGCATGCTTAGATGAAGTTTTGGGATTTGCTGTATGTGCTGTTTTTGTAGTCCATGAGCACCATCCAGTGGATAAACTTTATATGGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGTGCAATGGAATAGAATTGGAAGTATATGGCACACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAATCAGATCACCTGTGGCTATTCTATGTATCTAGTGATAAGTACTTTGGTACAGAGAGGTGGCGTAACTGTTACGGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAGGGAGTGTGGAGTCCGTCTGATATATGAGGAAGATGTGCAAGTGTTGAACCAAACAACCACTCAATCAAGCAGCAGGATGTCTCCTTATGAGGATATATTGATTGGTTTTGACATTCCTGTTGCAGGGGAAACCAGTGCCACTGGTAGCAGAACTTTCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

895

Amino Acids

101.54

Weight (kDa)

5.78

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 143 - 213 6.2e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 346 - 479 3.8e-09 Leucine-rich repeat region
LRR_13 PF23286 402 - 487 2.9e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 705 - 851 1.8e-13 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 429, 2188
AccB7I CCANNNNNTGG 1 cut(s) 2248
AccI GTMKAC 1 cut(s) 1906
AciI CCGC 2 cut(s) 47, 1669
AclI AACGTT 1 cut(s) 1279
AclWI GGATC 4 cut(s) 29, 42, 492, 1612
AcsI RAATTY 8 cut(s) 790, 859, 988, 1099, 1751, 1963, 2270, 2375
AcuI CTGAAG 6 cut(s) 207, 719, 858, 2078, 2333, 2525
AfaI GTAC 5 cut(s) 5, 1402, 1933, 2432, 2440
AfiI CCNNNNNNNGG 4 cut(s) 530, 1682, 2248, 2636
AflII CTTAAG 1 cut(s) 895
AjiI CACGTC 1 cut(s) 397
AjnI CCWGG 6 cut(s) 91, 649, 873, 1350, 2116, 2382
AjuI GAANNNNNNNTTGG 4 cut(s) 513, 545, 1838, 1870
AluBI AGCT 4 cut(s) 11, 517, 957, 1840
AluI AGCT 4 cut(s) 11, 517, 957, 1840
Alw21I GWGCWC 2 cut(s) 374, 2241
Alw26I GTCTC 9 cut(s) 642, 678, 697, 862, 1034, 1178, 1298, 1864, 2598
AlwI GGATC 4 cut(s) 29, 42, 492, 1612
Ama87I CYCGRG 1 cut(s) 1256
AoxI GGCC 3 cut(s) 841, 1348, 2495
ApeKI GCWGC 1 cut(s) 2583
ApoI RAATTY 8 cut(s) 790, 859, 988, 1099, 1751, 1963, 2270, 2375
Asp700I GAANNNNTTC 5 cut(s) 192, 624, 718, 2063, 2100
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 4 cut(s) 89, 829, 2177, 2495
AsuC2I CCSGG 1 cut(s) 2499
AsuHPI GGTGA 4 cut(s) 1742, 1786, 2390, 2521
AvaI CYCGRG 1 cut(s) 1256
AvaII GGWCC 3 cut(s) 89, 829, 2177
BamHI GGATCC 1 cut(s) 34
BauI CACGAG 1 cut(s) 299
BbsI GAAGAC 4 cut(s) 687, 845, 1802, 2066
Bbv12I GWGCWC 2 cut(s) 374, 2241
BbvI GCAGC 1 cut(s) 2595
BccI CCATC 6 cut(s) 1412, 1480, 1903, 1919, 2154, 2250
BciT130I CCWGG 6 cut(s) 93, 651, 875, 1352, 2118, 2384
BciVI GTATCC 1 cut(s) 900
BcnI CCSGG 1 cut(s) 2499
BcoDI GTCTC 9 cut(s) 642, 678, 697, 862, 1034, 1178, 1298, 1864, 2598
BfaI CTAG 5 cut(s) 503, 518, 1818, 1910, 2421
BfmI CTRYAG 2 cut(s) 1375, 1524
BfrI CTTAAG 1 cut(s) 895
BfuAI ACCTGC 2 cut(s) 429, 2188
BfuI GTATCC 1 cut(s) 900
BglII AGATCT 3 cut(s) 554, 899, 1081
BisI GCNGC 1 cut(s) 2584
BlsI GCNGC 1 cut(s) 2585
BmcAI AGTACT 1 cut(s) 2432
Bme1390I CCNGG 7 cut(s) 93, 651, 875, 1352, 2118, 2384, 2499
Bme18I GGWCC 3 cut(s) 89, 829, 2177
BmeT110I CYCGRG 1 cut(s) 1256
BmgBI CACGTC 1 cut(s) 397
BmgT120I GGNCC 4 cut(s) 89, 829, 2177, 2495
BmiI GGNNCC 3 cut(s) 36, 313, 2178
BmrFI CCNGG 7 cut(s) 93, 651, 875, 1352, 2118, 2384, 2499
BmrI ACTGGG 1 cut(s) 894
BmsI GCATC 8 cut(s) 142, 259, 741, 1436, 1577, 1945, 1967, 2021
BmuI ACTGGG 1 cut(s) 894
BpiI GAAGAC 4 cut(s) 687, 845, 1802, 2066
Bpu10I CCTNAGC 2 cut(s) 279, 365
BpuEI CTTGAG 4 cut(s) 886, 1091, 1160, 1862
BpuMI CCSGG 1 cut(s) 2499
BsaBI GATNNNNATC 1 cut(s) 483
BsaI GGTCTC 2 cut(s) 678, 1034
BsaJI CCNNGG 4 cut(s) 38, 92, 727, 1123
Bsc4I CCNNNNNNNGG 4 cut(s) 530, 1682, 2248, 2636
Bse1I ACTGG 5 cut(s) 86, 889, 2246, 2646, 2659
Bse3DI GCAATG 2 cut(s) 960, 2326
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 6 cut(s) 93, 651, 875, 1352, 2118, 2384
BseDI CCNNGG 4 cut(s) 38, 92, 727, 1123
BseGI GGATG 7 cut(s) 1097, 1451, 1592, 2036, 2242, 2272, 2595
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 4 cut(s) 530, 1682, 2248, 2636
BseMI GCAATG 2 cut(s) 960, 2326
BseMII CTCAG 4 cut(s) 293, 1358, 1703, 2124
BseNI ACTGG 5 cut(s) 86, 889, 2246, 2646, 2659
BseXI GCAGC 1 cut(s) 2595
BseYI CCCAGC 1 cut(s) 615
BshFI GGCC 3 cut(s) 843, 1350, 2497
BsiHKAI GWGCWC 2 cut(s) 374, 2241
BsiHKCI CYCGRG 1 cut(s) 1256
BsiSI CCGG 1 cut(s) 2498
BslFI GGGAC 3 cut(s) 63, 213, 2190
BslI CCNNNNNNNGG 4 cut(s) 530, 1682, 2248, 2636
BsmAI GTCTC 9 cut(s) 642, 678, 697, 862, 1034, 1178, 1298, 1864, 2598
BsmFI GGGAC 3 cut(s) 63, 213, 2190
BsnI GGCC 3 cut(s) 843, 1350, 2497
Bso31I GGTCTC 2 cut(s) 678, 1034
BsoBI CYCGRG 1 cut(s) 1256
Bsp1286I GDGCHC 2 cut(s) 374, 2241
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 9 cut(s) 34, 484, 554, 899, 928, 1081, 1549, 1604, 2395
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 2 cut(s) 47, 1669
BspANI GGCC 3 cut(s) 843, 1350, 2497
BspCNI CTCAG 4 cut(s) 292, 1359, 1704, 2125
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 3 cut(s) 36, 313, 2178
BspMAI CTGCAG 2 cut(s) 1379, 1528
BspMI ACCTGC 2 cut(s) 429, 2188
BspPI GGATC 4 cut(s) 29, 42, 492, 1612
BspTI CTTAAG 1 cut(s) 895
BspTNI GGTCTC 2 cut(s) 678, 1034
BsrDI GCAATG 2 cut(s) 960, 2326
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 86, 889, 2246, 2646, 2659
BssECI CCNNGG 4 cut(s) 38, 92, 727, 1123
BssMI GATC 9 cut(s) 34, 484, 554, 899, 928, 1081, 1549, 1604, 2395
BssNAI GTATAC 1 cut(s) 1907
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 3 cut(s) 38, 727, 1123
Bst1107I GTATAC 1 cut(s) 1907
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 6 cut(s) 93, 651, 875, 1352, 2118, 2384
Bst4CI ACNGT 5 cut(s) 585, 636, 1493, 2459, 2465
Bst6I CTCTTC 1 cut(s) 695
BstAFI CTTAAG 1 cut(s) 895
BstAPI GCANNNNNTGC 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 6 cut(s) 45, 460, 515, 1673, 2185, 2355
BstDEI CTNAG 8 cut(s) 279, 365, 1367, 1712, 1787, 1836, 2133, 2188
BstDSI CCRYGG 1 cut(s) 38
BstEII GGTNACC 1 cut(s) 1774
BstENI CCTNNNNNAGG 1 cut(s) 2634
BstF5I GGATG 7 cut(s) 1097, 1451, 1592, 2036, 2242, 2272, 2595
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 9 cut(s) 37, 487, 557, 902, 931, 1084, 1552, 1607, 2398
BstMAI GTCTC 9 cut(s) 642, 678, 697, 862, 1034, 1178, 1298, 1864, 2598
BstMBI GATC 9 cut(s) 34, 484, 554, 899, 928, 1081, 1549, 1604, 2395
BstMWI GCNNNNNNNGC 4 cut(s) 598, 1837, 2216, 2316
BstNI CCWGG 6 cut(s) 93, 651, 875, 1352, 2118, 2384
BstNSI RCATGY 3 cut(s) 47, 462, 2187
BstPI GGTNACC 1 cut(s) 1774
BstSCI CCNGG 7 cut(s) 91, 649, 873, 1350, 2116, 2382, 2497
BstSFI CTRYAG 2 cut(s) 1375, 1524
BstV1I GCAGC 1 cut(s) 2595
BstV2I GAAGAC 4 cut(s) 687, 845, 1802, 2066
BstX2I RGATCY 5 cut(s) 34, 554, 899, 1081, 1604
BstYI RGATCY 5 cut(s) 34, 554, 899, 1081, 1604
BstZ17I GTATAC 1 cut(s) 1907
BsuI GTATCC 1 cut(s) 900
BsuRI GGCC 3 cut(s) 843, 1350, 2497
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 1 cut(s) 397
BtsCI GGATG 7 cut(s) 1097, 1451, 1592, 2036, 2242, 2272, 2595
BtsIMutI CAGTG 4 cut(s) 1937, 2253, 2652, 2653
BveI ACCTGC 2 cut(s) 429, 2188
Cac8I GCNNGC 6 cut(s) 45, 460, 515, 1673, 2185, 2355
CciI TCATGA 1 cut(s) 676
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 4 cut(s) 89, 829, 2177, 2495
Csp6I GTAC 5 cut(s) 4, 1401, 1932, 2431, 2439
CviQI GTAC 5 cut(s) 4, 1401, 1932, 2431, 2439
DdeI CTNAG 8 cut(s) 279, 365, 1367, 1712, 1787, 1836, 2133, 2188
DpnI GATC 9 cut(s) 36, 486, 556, 901, 930, 1083, 1551, 1606, 2397
DpnII GATC 9 cut(s) 34, 484, 554, 899, 928, 1081, 1549, 1604, 2395
DraI TTTAAA 1 cut(s) 1917
Eam1104I CTCTTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 695
Eco130I CCWWGG 3 cut(s) 38, 727, 1123
Eco147I AGGCCT 1 cut(s) 843
Eco31I GGTCTC 2 cut(s) 678, 1034
Eco47I GGWCC 3 cut(s) 89, 829, 2177
Eco57I CTGAAG 6 cut(s) 207, 719, 858, 2078, 2333, 2525
Eco88I CYCGRG 1 cut(s) 1256
Eco91I GGTNACC 1 cut(s) 1774
EcoNI CCTNNNNNAGG 1 cut(s) 2634
EcoO109I RGGNCCY 1 cut(s) 2177
EcoO65I GGTNACC 1 cut(s) 1774
EcoRI GAATTC 1 cut(s) 2270
EcoRII CCWGG 6 cut(s) 91, 649, 873, 1350, 2116, 2382
EcoT14I CCWWGG 3 cut(s) 38, 727, 1123
EcoT22I ATGCAT 2 cut(s) 594, 1960
ErhI CCWWGG 3 cut(s) 38, 727, 1123
FaqI GGGAC 3 cut(s) 63, 213, 2190
FauI CCCGC 1 cut(s) 40
FauNDI CATATG 1 cut(s) 2106
FblI GTMKAC 1 cut(s) 1906
Fnu4HI GCNGC 1 cut(s) 2584
FokI GGATG 7 cut(s) 1104, 1458, 1599, 2043, 2229, 2279, 2602
Fsp4HI GCNGC 1 cut(s) 2584
FspBI CTAG 5 cut(s) 503, 518, 1818, 1910, 2421
GlaI GCGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 2584
GsaI CCCAGC 1 cut(s) 619
HaeIII GGCC 3 cut(s) 843, 1350, 2497
HapII CCGG 1 cut(s) 2498
HhaI GCGC 1 cut(s) 142
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 1 cut(s) 747
HindII GTYRAC 1 cut(s) 747
HindIII AAGCTT 1 cut(s) 1838
HpaII CCGG 1 cut(s) 2498
HphI GGTGA 4 cut(s) 1742, 1786, 2390, 2521
Hpy166II GTNNAC 5 cut(s) 6, 632, 747, 829, 1907
Hpy8I GTNNAC 5 cut(s) 6, 632, 747, 829, 1907
HpyAV CCTTC 9 cut(s) 205, 833, 842, 1082, 1635, 1727, 1824, 1901, 2500
HpyCH4III ACNGT 5 cut(s) 585, 636, 1493, 2459, 2465
HpyCH4IV ACGT 3 cut(s) 396, 658, 1279
HpyF10VI GCNNNNNNNGC 4 cut(s) 598, 1837, 2216, 2316
HpyF3I CTNAG 8 cut(s) 279, 365, 1367, 1712, 1787, 1836, 2133, 2188
HpySE526I ACGT 3 cut(s) 396, 658, 1279
HspAI GCGC 1 cut(s) 140
Kzo9I GATC 9 cut(s) 34, 484, 554, 899, 928, 1081, 1549, 1604, 2395
LmnI GCTCC 2 cut(s) 369, 1736
Lsp1109I GCAGC 1 cut(s) 2595
LweI GCATC 8 cut(s) 142, 259, 741, 1436, 1577, 1945, 1967, 2021
MaeI CTAG 5 cut(s) 503, 518, 1818, 1910, 2421
MaeII ACGT 3 cut(s) 396, 658, 1279
MaeIII GTNAC 5 cut(s) 490, 760, 1774, 2453, 2459
MalI GATC 9 cut(s) 36, 486, 556, 901, 930, 1083, 1551, 1606, 2397
MboI GATC 9 cut(s) 34, 484, 554, 899, 928, 1081, 1549, 1604, 2395
MfeI CAATTG 2 cut(s) 1332, 2002
MflI RGATCY 5 cut(s) 34, 554, 899, 1081, 1604
MhlI GDGCHC 2 cut(s) 374, 2241
MlyI GAGTC 4 cut(s) 757, 864, 1174, 2532
MmeI TCCRAC 2 cut(s) 597, 1246
Mph1103I ATGCAT 2 cut(s) 594, 1960
MroXI GAANNNNTTC 5 cut(s) 192, 624, 718, 2063, 2100
MslI CAYNNNNRTG 2 cut(s) 737, 2151
MspCI CTTAAG 1 cut(s) 895
MspI CCGG 1 cut(s) 2498
MspR9I CCNGG 7 cut(s) 93, 651, 875, 1352, 2118, 2384, 2499
MunI CAATTG 2 cut(s) 1332, 2002
MvaI CCWGG 6 cut(s) 93, 651, 875, 1352, 2118, 2384
MwoI GCNNNNNNNGC 4 cut(s) 598, 1837, 2216, 2316
NciI CCSGG 1 cut(s) 2499
NcoI CCATGG 1 cut(s) 38
NdeI CATATG 1 cut(s) 2106
NdeII GATC 9 cut(s) 34, 484, 554, 899, 928, 1081, 1549, 1604, 2395
NlaIV GGNNCC 3 cut(s) 36, 313, 2178
NmuCI GTSAC 3 cut(s) 490, 760, 1774
NsiI ATGCAT 2 cut(s) 594, 1960
NspI RCATGY 3 cut(s) 47, 462, 2187
PaeI GCATGC 3 cut(s) 47, 462, 2187
PagI TCATGA 1 cut(s) 676
PceI AGGCCT 1 cut(s) 843
PdmI GAANNNNTTC 5 cut(s) 192, 624, 718, 2063, 2100
PflMI CCANNNNNTGG 1 cut(s) 2248
PfoI TCCNGGA 2 cut(s) 873, 2116
PkrI GCNGC 1 cut(s) 2585
PleI GAGTC 4 cut(s) 757, 864, 1174, 2531
PpsI GAGTC 4 cut(s) 757, 864, 1174, 2531
PpuMI RGGWCCY 1 cut(s) 2177
Psp1406I AACGTT 1 cut(s) 1279
Psp5II RGGWCCY 1 cut(s) 2177
Psp6I CCWGG 6 cut(s) 91, 649, 873, 1350, 2116, 2382
PspEI GGTNACC 1 cut(s) 1774
PspFI CCCAGC 1 cut(s) 615
PspGI CCWGG 6 cut(s) 91, 649, 873, 1350, 2116, 2382
PspN4I GGNNCC 3 cut(s) 36, 313, 2178
PspPI GGNCC 4 cut(s) 89, 829, 2177, 2495
PspPPI RGGWCCY 1 cut(s) 2177
PstI CTGCAG 2 cut(s) 1379, 1528
PsuI RGATCY 5 cut(s) 34, 554, 899, 1081, 1604
RsaI GTAC 5 cut(s) 5, 1402, 1933, 2432, 2440
RsaNI GTAC 5 cut(s) 4, 1401, 1932, 2431, 2439
RseI CAYNNNNRTG 2 cut(s) 737, 2151
SatI GCNGC 1 cut(s) 2584
Sau3AI GATC 9 cut(s) 34, 484, 554, 899, 928, 1081, 1549, 1604, 2395
Sau96I GGNCC 4 cut(s) 89, 829, 2177, 2495
ScaI AGTACT 1 cut(s) 2432
SchI GAGTC 4 cut(s) 757, 864, 1174, 2532
ScrFI CCNGG 7 cut(s) 93, 651, 875, 1352, 2118, 2384, 2499
SduI GDGCHC 2 cut(s) 374, 2241
SfaNI GCATC 8 cut(s) 142, 259, 741, 1436, 1577, 1945, 1967, 2021
SfcI CTRYAG 2 cut(s) 1375, 1524
SinI GGWCC 3 cut(s) 89, 829, 2177
SmiMI CAYNNNNRTG 2 cut(s) 737, 2151
SmlI CTYRAG 5 cut(s) 865, 895, 1070, 1175, 1841
SmoI CTYRAG 5 cut(s) 865, 895, 1070, 1175, 1841
SphI GCATGC 3 cut(s) 47, 462, 2187
SseBI AGGCCT 1 cut(s) 843
SsiI CCGC 2 cut(s) 47, 1669
SspI AATATT 3 cut(s) 444, 509, 2128
SspMI CTAG 5 cut(s) 503, 518, 1818, 1910, 2421
StuI AGGCCT 1 cut(s) 843
StyD4I CCNGG 7 cut(s) 91, 649, 873, 1350, 2116, 2382, 2497
StyI CCWWGG 3 cut(s) 38, 727, 1123
TaaI ACNGT 5 cut(s) 585, 636, 1493, 2459, 2465
TaiI ACGT 3 cut(s) 399, 661, 1282
TatI WGTACW 3 cut(s) 3, 1931, 2430
TscAI CASTG 4 cut(s) 1944, 2253, 2653, 2659
TseFI GTSAC 3 cut(s) 490, 760, 1774
TseI GCWGC 1 cut(s) 2583
Tsp45I GTSAC 3 cut(s) 490, 760, 1774
TspDTI ATGAA 9 cut(s) 693, 809, 997, 1987, 2056, 2093, 2154, 2208, 2283
TspGWI ACGGA 4 cut(s) 279, 993, 1986, 2516
TspRI CASTG 4 cut(s) 1944, 2253, 2653, 2659
Van91I CCANNNNNTGG 1 cut(s) 2248
Vha464I CTTAAG 1 cut(s) 895
VpaK11BI GGWCC 3 cut(s) 89, 829, 2177
XagI CCTNNNNNAGG 1 cut(s) 2634
XapI RAATTY 8 cut(s) 790, 859, 988, 1099, 1751, 1963, 2270, 2375
XceI RCATGY 3 cut(s) 47, 462, 2187
XcmI CCANNNNNNNNNTGG 2 cut(s) 1121, 2153
XmiI GTMKAC 1 cut(s) 1906
XmnI GAANNNNTTC 5 cut(s) 192, 624, 718, 2063, 2100
XspI CTAG 5 cut(s) 503, 518, 1818, 1910, 2421
ZrmI AGTACT 1 cut(s) 2432
Zsp2I ATGCAT 2 cut(s) 594, 1960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.