RLG00000015527

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000105
Physical Location & Seq
Reverse (-)
67539553 .. 67546036
6484 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015527

Sequence Viewer

Length: 2430 bp
ATGCATGGAAGAGAGGGGACAATTAGTCTAGGGGATCTTCTACAAAGTGGATCCTTCAGATGTGCGCCACCAAAGGGGAAGTTTTCAGCTCCGTCCAGTAGAGGGAGGGATAGAATTAATACGAAGGGAGTGCCTATTTGTAGTCAGATTATCATCAGATTTCAACTGCAAACTCTTGTAGAGTTGAGAGATCCTCGGGATGATGGACAATTGATTCAGGACATTGTTAGGGAGGTACTAGATAAACTAGGTGCTACTTATACCTCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTGTTAAGAAATTACCTACGTCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCAAAGCTGCATTCTTTCCAATGTTAGAGAAAAATCTGAAAAGGATGGCCTAGTTTCTCTTCAAGAAAAACTACTTTCCAGAATCCTGAGGACAAAAGTTGACATTGAGGATGAATATGCAGGAGCTACTATGATAGAAAGGCGGTTATATATTCATTTGCTAAGGATTGATGATACATACAAGGCTACTGGGTTAGCTCGTGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAAAAGCTTCCCACCAGAAGATTATTTGGATTTGTGCCACCATATTATAGGGTATGCTCAGGGGCTGCCATTGGCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGACCCAATGAATGGGAAAGTGCAATAGAGAGGCTAAACAATGCACCAAATAGACAAGTTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGAAAAAGACAAACAGATATTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGGCTATTGCAAGCTAGAACCTGGCATTGTTCATCGAGAGTCCCCTGATGAGCCAGGTAAACGAAGTAGATTATGGTCTCCTGAAGATATCCACAAAGTTCTGAAGAAAAATAAGAATTTTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCAGACCCCAGACCTCAGAGGCGTTCAAACTCTTGAGACTTTAGATCTTGAAGGATGTAAGACTTTGGTAAAACTTCATCAATCCCTCGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAAGATTGCGAGAGTCTCGAGAGTCTGCCAAGTAAGATTGAAATGGAATCTCTTCAAACTTTAATTCTTTCTAACTGCTCAAAAGTTAAAAGGATTCCTGAGTTCGCTGGAAATATGGAGCTTTTATCAGAGCTTTATCTCGATGAGACTGCCATTGAAGAACTACCTGTTTCAATTGGATGCCTGAGTGGCCTTACTTCACTGAATCTGAGCAACTGCAAAAATCTTCTATGCCTTCCAAGCACCTTTAGTAGATTGGAGTCTCTTGAAAAGCTTAATCTTTCTGGATGTTTGAAACTTGGAGAACAGGATAGTGCGAGGGAGAATGGTGCAACTAGAACTGCTGTAAATTATCTCACAAAACGCTTTTGTGAAGTTGTTGGGGAATATTTGTCATGGTCCTTGCCTTCTAGATTGGTGCAAAGAACCGATATAGAACCAATGAGTATGCAGTTGCCTGTATCTGATCTGTGTTATTCTACAGAGCAAAATACAAATACATCAATTAGTATCCGGTTGCCACTATCTCGTCTATGTAATTTAACAGATCTAAACATAAGTAACTGCAATCTTGGTGACGCTGCATTTGCCAACTTTGCCTGTTTTCCCTCCTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTAAGCTTCAGAACATTAATTTGGAGAATTGCAAGACACTTCAAGAGTTGTCAGGACTTCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAAATAGTGTTTGATGTATCCAATTTCAATATATTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTGCAGTAACATAGCATTTGAAATGCTGAGGATATTCCTTAATCAGAACCTTGACCTCAATCAACCATCCGGTTCTTCCCACACCTCCCAGCCTCATCCTGCAAACCAATACTCTTCTCGGCGAAGTAGGGGCCCTCGCCACGCGCGGCCAACTCAACGAAGCGCTCTCACTCTTCTACACTCTTCAGCCGCCACCCTCTCACTGCAACCAAACCTAAAATTATATGCACCTAAAATTAAGAAAAGCGTGAGAATTGGATCATACTACATCACCGAATATCCTCTATTATCAGAAGAGAAGGCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

810

Amino Acids

90.79

Weight (kDa)

8.61

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 116 - 197 2.1e-06 NB-ARC domain
LRR_13 PF23286 432 - 518 7.5e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 437 - 511 5.3e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 801
AccII CGCG 2 cut(s) 2264, 2266
AciI CCGC 6 cut(s) 304, 338, 379, 583, 2266, 2310
AclWI GGATC 8 cut(s) 42, 45, 58, 185, 361, 374, 951, 2386
AcoI YGGCCR 1 cut(s) 2267
AcsI RAATTY 2 cut(s) 423, 1078
AcuI CTGAAG 6 cut(s) 40, 845, 1065, 1085, 1916, 2289
AfaI GTAC 2 cut(s) 237, 926
AfeI AGCGCT 1 cut(s) 2284
AfiI CCNNNNNNNGG 4 cut(s) 74, 102, 344, 801
AhdI GACNNNNNGTC 1 cut(s) 24
AjnI CCWGG 2 cut(s) 982, 1015
AjuI GAANNNNNNNTTGG 4 cut(s) 435, 467, 1928, 1960
Alw26I GTCTC 5 cut(s) 1044, 1157, 1268, 1388, 1515
AlwI GGATC 8 cut(s) 42, 45, 58, 185, 361, 374, 951, 2386
AlwNI CAGNNNCTG 2 cut(s) 745, 1920
Ama87I CYCGRG 3 cut(s) 195, 405, 1265
Aor51HI AGCGCT 1 cut(s) 2284
AoxI GGCC 5 cut(s) 487, 1438, 2251, 2267, 2421
ApaI GGGCCC 1 cut(s) 2255
ApeKI GCWGC 5 cut(s) 340, 447, 745, 1829, 2121
ApoI RAATTY 2 cut(s) 423, 1078
ArsI GACNNNNNNTTYG 2 cut(s) 1817, 1849
AseI ATTAAT 2 cut(s) 117, 1941
Asp700I GAANNNNTTC 4 cut(s) 298, 651, 686, 1299
AspLEI GCGC 3 cut(s) 67, 2266, 2285
AspS9I GGNCC 4 cut(s) 1647, 1917, 2251, 2252
AsuHPI GGTGA 3 cut(s) 656, 1835, 2383
AvaI CYCGRG 3 cut(s) 195, 405, 1265
AvaII GGWCC 2 cut(s) 1647, 1917
AxyI CCTNAGG 1 cut(s) 527
BaeGI GKGCMC 1 cut(s) 2255
BamHI GGATCC 2 cut(s) 50, 366
BanII GRGCYC 1 cut(s) 2255
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 1 cut(s) 796
BbvCI CCTCAGC 1 cut(s) 2147
BbvI GCAGC 5 cut(s) 352, 434, 732, 1816, 2108
BccI CCATC 6 cut(s) 197, 479, 869, 1993, 2009, 2194
BcgI CGANNNNNNTGC 4 cut(s) 112, 146, 1379, 1413
BciT130I CCWGG 2 cut(s) 984, 1017
BciVI GTATCC 2 cut(s) 1769, 2059
BcoDI GTCTC 5 cut(s) 1044, 1157, 1268, 1388, 1515
BfaI CTAG 9 cut(s) 29, 239, 248, 491, 881, 962, 977, 1584, 1659
BfmI CTRYAG 2 cut(s) 1728, 2122
BfoI RGCGCY 1 cut(s) 2286
BfuI GTATCC 2 cut(s) 1769, 2059
BglI GCCNNNNNGGC 1 cut(s) 1437
BglII AGATCT 2 cut(s) 1171, 1795
BisI GCNGC 7 cut(s) 341, 448, 746, 1830, 2122, 2267, 2310
BlsI GCNGC 7 cut(s) 342, 449, 747, 1831, 2123, 2268, 2311
Bme1390I CCNGG 2 cut(s) 984, 1017
Bme18I GGWCC 2 cut(s) 1647, 1917
BmeRI GACNNNNNGTC 1 cut(s) 24
BmeT110I CYCGRG 3 cut(s) 195, 405, 1265
BmgT120I GGNCC 4 cut(s) 1647, 1917, 2251, 2252
BmiI GGNNCC 4 cut(s) 52, 368, 2252, 2253
BmrFI CCNGG 2 cut(s) 984, 1017
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 2 cut(s) 1418, 1920
BmuI ACTGGG 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 796
BplI GAGNNNNNCTC 2 cut(s) 178, 210
Bpu10I CCTNAGC 3 cut(s) 602, 738, 2147
BpuEI CTTGAG 1 cut(s) 1181
BsaBI GATNNNNATC 1 cut(s) 152
BsaI GGTCTC 1 cut(s) 1044
BsaJI CCNNGG 3 cut(s) 194, 370, 1213
BsaWI WCCGGW 2 cut(s) 1761, 2189
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 102, 344, 801
Bse1I ACTGG 3 cut(s) 96, 634, 2034
Bse21I CCTNAGG 1 cut(s) 527
Bse8I GATNNNNATC 1 cut(s) 152
BseBI CCWGG 2 cut(s) 984, 1017
BseDI CCNNGG 3 cut(s) 194, 370, 1213
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 4 cut(s) 74, 102, 344, 801
BseMII CTCAG 8 cut(s) 518, 752, 1156, 1338, 1424, 1448, 1868, 2138
BseNI ACTGG 3 cut(s) 96, 634, 2034
BseSI GKGCMC 1 cut(s) 2255
BseXI GCAGC 5 cut(s) 352, 434, 732, 1816, 2108
BseYI CCCAGC 1 cut(s) 2208
Bsh1236I CGCG 2 cut(s) 2264, 2266
BshFI GGCC 5 cut(s) 489, 1440, 2253, 2269, 2423
BsiHKCI CYCGRG 3 cut(s) 195, 405, 1265
BsiSI CCGG 2 cut(s) 1762, 2190
BslFI GGGAC 2 cut(s) 31, 988
BslI CCNNNNNNNGG 4 cut(s) 74, 102, 344, 801
BsmAI GTCTC 5 cut(s) 1044, 1157, 1268, 1388, 1515
BsmFI GGGAC 2 cut(s) 31, 988
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 5 cut(s) 489, 1440, 2253, 2269, 2423
Bso31I GGTCTC 1 cut(s) 1044
BsoBI CYCGRG 3 cut(s) 195, 405, 1265
Bsp120I GGGCCC 1 cut(s) 2251
Bsp1286I GDGCHC 1 cut(s) 2255
Bsp1407I TGTACA 1 cut(s) 924
Bsp143I GATC 9 cut(s) 34, 50, 190, 366, 943, 1171, 1714, 1795, 2378
Bsp19I CCATGG 1 cut(s) 370
BspACI CCGC 6 cut(s) 304, 338, 379, 583, 2266, 2310
BspANI GGCC 5 cut(s) 489, 1440, 2253, 2269, 2423
BspCNI CTCAG 8 cut(s) 519, 751, 1155, 1339, 1425, 1449, 1869, 2139
BspFNI CGCG 2 cut(s) 2264, 2266
BspLI GGNNCC 4 cut(s) 52, 368, 2252, 2253
BspMAI CTGCAG 1 cut(s) 2126
BspPI GGATC 8 cut(s) 42, 45, 58, 185, 361, 374, 951, 2386
BspTNI GGTCTC 1 cut(s) 1044
BsrGI TGTACA 1 cut(s) 924
BsrI ACTGG 3 cut(s) 96, 634, 2034
BssECI CCNNGG 3 cut(s) 194, 370, 1213
BssMI GATC 9 cut(s) 34, 50, 190, 366, 943, 1171, 1714, 1795, 2378
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 370
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 2 cut(s) 984, 1017
Bst6I CTCTTC 8 cut(s) 4, 271, 504, 1305, 2239, 2298, 2308, 2409
BstAUI TGTACA 1 cut(s) 924
BstC8I GCNNGC 2 cut(s) 377, 974
BstDSI CCRYGG 1 cut(s) 370
BstFNI CGCG 2 cut(s) 2264, 2266
BstH2I RGCGCY 1 cut(s) 2286
BstHHI GCGC 3 cut(s) 67, 2266, 2285
BstKTI GATC 9 cut(s) 37, 53, 193, 369, 946, 1174, 1717, 1798, 2381
BstMAI GTCTC 5 cut(s) 1044, 1157, 1268, 1388, 1515
BstMBI GATC 9 cut(s) 34, 50, 190, 366, 943, 1171, 1714, 1795, 2378
BstMWI GCNNNNNNNGC 5 cut(s) 381, 1437, 1488, 1835, 1844
BstNI CCWGG 2 cut(s) 984, 1017
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 982, 1015
BstSFI CTRYAG 2 cut(s) 1728, 2122
BstSLI GKGCMC 1 cut(s) 2255
BstUI CGCG 2 cut(s) 2264, 2266
BstV1I GCAGC 5 cut(s) 352, 434, 732, 1816, 2108
BstV2I GAAGAC 1 cut(s) 796
BstX2I RGATCY 6 cut(s) 34, 50, 190, 366, 1171, 1795
BstYI RGATCY 6 cut(s) 34, 50, 190, 366, 1171, 1795
Bsu36I CCTNAGG 1 cut(s) 527
BsuI GTATCC 2 cut(s) 1769, 2059
BsuRI GGCC 5 cut(s) 489, 1440, 2253, 2269, 2423
BtgI CCRYGG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 2321
BtsIMutI CAGTG 3 cut(s) 1448, 2027, 2321
Cac8I GCNNGC 2 cut(s) 377, 974
CaiI CAGNNNCTG 2 cut(s) 745, 1920
CfoI GCGC 3 cut(s) 67, 2266, 2285
Cfr13I GGNCC 4 cut(s) 1647, 1917, 2251, 2252
CseI GACGC 1 cut(s) 1835
Csp6I GTAC 2 cut(s) 236, 925
CviAII CATG 5 cut(s) 5, 371, 376, 416, 1644
CviQI GTAC 2 cut(s) 236, 925
DpnI GATC 9 cut(s) 36, 52, 192, 368, 945, 1173, 1716, 1797, 2380
DpnII GATC 9 cut(s) 34, 50, 190, 366, 943, 1171, 1714, 1795, 2378
DraI TTTAAA 1 cut(s) 669
DriI GACNNNNNGTC 1 cut(s) 24
EaeI YGGCCR 1 cut(s) 2267
Eam1104I CTCTTC 8 cut(s) 4, 271, 504, 1305, 2239, 2298, 2308, 2409
Eam1105I GACNNNNNGTC 1 cut(s) 24
EarI CTCTTC 8 cut(s) 4, 271, 504, 1305, 2239, 2298, 2308, 2409
Eco130I CCWWGG 1 cut(s) 370
Eco24I GRGCYC 1 cut(s) 2255
Eco31I GGTCTC 1 cut(s) 1044
Eco32I GATATC 1 cut(s) 1051
Eco47I GGWCC 2 cut(s) 1647, 1917
Eco47III AGCGCT 1 cut(s) 2284
Eco57I CTGAAG 6 cut(s) 40, 845, 1065, 1085, 1916, 2289
Eco81I CCTNAGG 1 cut(s) 527
Eco88I CYCGRG 3 cut(s) 195, 405, 1265
EcoO109I RGGNCCY 3 cut(s) 1917, 2251, 2252
EcoRII CCWGG 2 cut(s) 982, 1015
EcoRV GATATC 1 cut(s) 1051
EcoT14I CCWWGG 1 cut(s) 370
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 2255
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 5 cut(s) 8, 374, 379, 419, 1647
FalI AAGNNNNNCTT 2 cut(s) 671, 703
FaqI GGGAC 2 cut(s) 31, 988
FatI CATG 5 cut(s) 4, 370, 375, 415, 1643
FauI CCCGC 2 cut(s) 311, 372
Fnu4HI GCNGC 7 cut(s) 341, 448, 746, 1830, 2122, 2267, 2310
FriOI GRGCYC 1 cut(s) 2255
Fsp4HI GCNGC 7 cut(s) 341, 448, 746, 1830, 2122, 2267, 2310
FspBI CTAG 9 cut(s) 29, 239, 248, 491, 881, 962, 977, 1584, 1659
GlaI GCGC 3 cut(s) 66, 2265, 2284
GluI GCNGC 7 cut(s) 341, 448, 746, 1830, 2122, 2267, 2310
GsaI CCCAGC 1 cut(s) 2212
HaeII RGCGCY 1 cut(s) 2286
HaeIII GGCC 5 cut(s) 489, 1440, 2253, 2269, 2423
HapII CCGG 2 cut(s) 1762, 2190
HgaI GACGC 1 cut(s) 1835
HhaI GCGC 3 cut(s) 67, 2266, 2285
Hin1II CATG 5 cut(s) 8, 374, 379, 419, 1647
Hin6I GCGC 3 cut(s) 65, 2264, 2283
HinP1I GCGC 3 cut(s) 65, 2264, 2283
HincII GTYRAC 2 cut(s) 277, 541
HindII GTYRAC 2 cut(s) 277, 541
HindIII AAGCTT 4 cut(s) 685, 1520, 1898, 1928
HpaII CCGG 2 cut(s) 1762, 2190
HphI GGTGA 3 cut(s) 656, 1835, 2383
Hpy166II GTNNAC 3 cut(s) 277, 541, 1022
Hpy8I GTNNAC 3 cut(s) 277, 541, 1022
HpyAV CCTTC 7 cut(s) 64, 118, 928, 1172, 1493, 1665, 2413
HpyCH4IV ACGT 1 cut(s) 334
HpyF10VI GCNNNNNNNGC 5 cut(s) 381, 1437, 1488, 1835, 1844
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 5 cut(s) 8, 374, 379, 419, 1647
HspAI GCGC 3 cut(s) 65, 2264, 2283
Kzo9I GATC 9 cut(s) 34, 50, 190, 366, 943, 1171, 1714, 1795, 2378
LmnI GCTCC 3 cut(s) 94, 563, 1366
Lsp1109I GCAGC 5 cut(s) 352, 434, 732, 1816, 2108
LweI GCATC 2 cut(s) 1418, 1920
MaeI CTAG 9 cut(s) 29, 239, 248, 491, 881, 962, 977, 1584, 1659
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 4 cut(s) 949, 1808, 1823, 2126
MalI GATC 9 cut(s) 36, 52, 192, 368, 945, 1173, 1716, 1797, 2380
MboI GATC 9 cut(s) 34, 50, 190, 366, 943, 1171, 1714, 1795, 2378
MfeI CAATTG 3 cut(s) 209, 1422, 2092
MflI RGATCY 6 cut(s) 34, 50, 190, 366, 1171, 1795
MhlI GDGCHC 1 cut(s) 2255
MlyI GAGTC 4 cut(s) 1010, 1270, 1279, 1517
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 4 cut(s) 298, 651, 686, 1299
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 2 cut(s) 1762, 2190
MspR9I CCNGG 2 cut(s) 984, 1017
MunI CAATTG 3 cut(s) 209, 1422, 2092
Mva1269I GAATGC 1 cut(s) 450
MvaI CCWGG 2 cut(s) 984, 1017
MvnI CGCG 2 cut(s) 2264, 2266
MwoI GCNNNNNNNGC 5 cut(s) 381, 1437, 1488, 1835, 1844
NcoI CCATGG 1 cut(s) 370
NdeII GATC 9 cut(s) 34, 50, 190, 366, 943, 1171, 1714, 1795, 2378
NlaIII CATG 5 cut(s) 8, 374, 379, 419, 1647
NlaIV GGNNCC 4 cut(s) 52, 368, 2252, 2253
NmeAIII GCCGAG 1 cut(s) 2218
NmuCI GTSAC 2 cut(s) 949, 1823
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 379
PaeI GCATGC 1 cut(s) 379
PaeR7I CTCGAG 2 cut(s) 405, 1265
PctI GAATGC 1 cut(s) 450
PdmI GAANNNNTTC 4 cut(s) 298, 651, 686, 1299
PfeI GAWTC 7 cut(s) 214, 299, 522, 1243, 1295, 1342, 1453
PflMI CCANNNNNTGG 1 cut(s) 801
PkrI GCNGC 7 cut(s) 342, 449, 747, 1831, 2123, 2268, 2311
PleI GAGTC 4 cut(s) 1009, 1269, 1278, 1516
PpsI GAGTC 4 cut(s) 1009, 1269, 1278, 1516
PpuMI RGGWCCY 1 cut(s) 1917
PshBI ATTAAT 2 cut(s) 117, 1941
Psp5II RGGWCCY 1 cut(s) 1917
Psp6I CCWGG 2 cut(s) 982, 1015
PspFI CCCAGC 1 cut(s) 2208
PspGI CCWGG 2 cut(s) 982, 1015
PspN4I GGNNCC 4 cut(s) 52, 368, 2252, 2253
PspOMI GGGCCC 1 cut(s) 2251
PspPI GGNCC 4 cut(s) 1647, 1917, 2251, 2252
PspPPI RGGWCCY 1 cut(s) 1917
PspXI VCTCGAGB 1 cut(s) 405
PstI CTGCAG 1 cut(s) 2126
PstNI CAGNNNCTG 2 cut(s) 745, 1920
PsuI RGATCY 6 cut(s) 34, 50, 190, 366, 1171, 1795
RsaI GTAC 2 cut(s) 237, 926
RsaNI GTAC 2 cut(s) 236, 925
RseI CAYNNNNRTG 1 cut(s) 293
SatI GCNGC 7 cut(s) 341, 448, 746, 1830, 2122, 2267, 2310
Sau3AI GATC 9 cut(s) 34, 50, 190, 366, 943, 1171, 1714, 1795, 2378
Sau96I GGNCC 4 cut(s) 1647, 1917, 2251, 2252
SchI GAGTC 4 cut(s) 1010, 1270, 1279, 1517
ScrFI CCNGG 2 cut(s) 984, 1017
SduI GDGCHC 1 cut(s) 2255
SfaNI GCATC 2 cut(s) 1418, 1920
SfcI CTRYAG 2 cut(s) 1728, 2122
Sfr274I CTCGAG 2 cut(s) 405, 1265
SinI GGWCC 2 cut(s) 1647, 1917
SlaI CTCGAG 2 cut(s) 405, 1265
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 3 cut(s) 405, 1160, 1265
SmoI CTYRAG 3 cut(s) 405, 1160, 1265
SphI GCATGC 1 cut(s) 379
SsiI CCGC 6 cut(s) 304, 338, 379, 583, 2266, 2310
SspI AATATT 1 cut(s) 1637
SspMI CTAG 9 cut(s) 29, 239, 248, 491, 881, 962, 977, 1584, 1659
StyD4I CCNGG 2 cut(s) 982, 1015
StyI CCWWGG 1 cut(s) 370
TaiI ACGT 1 cut(s) 337
TaqI TCGA 4 cut(s) 406, 997, 1266, 1389
TatI WGTACW 1 cut(s) 924
TauI GCSGC 2 cut(s) 2269, 2312
TfiI GAWTC 7 cut(s) 214, 299, 522, 1243, 1295, 1342, 1453
TscAI CASTG 3 cut(s) 1455, 2034, 2328
TseFI GTSAC 2 cut(s) 949, 1823
TseI GCWGC 5 cut(s) 340, 447, 745, 1829, 2121
Tsp45I GTSAC 2 cut(s) 949, 1823
TspDTI ATGAA 9 cut(s) 277, 435, 567, 584, 813, 983, 1087, 1193, 2077
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 3 cut(s) 1455, 2034, 2328
Van91I CCANNNNNTGG 1 cut(s) 801
VpaK11BI GGWCC 2 cut(s) 1647, 1917
VspI ATTAAT 2 cut(s) 117, 1941
XapI RAATTY 2 cut(s) 423, 1078
XbaI TCTAGA 1 cut(s) 1658
XceI RCATGY 1 cut(s) 379
XhoI CTCGAG 2 cut(s) 405, 1265
XmnI GAANNNNTTC 4 cut(s) 298, 651, 686, 1299
XspI CTAG 9 cut(s) 29, 239, 248, 491, 881, 962, 977, 1584, 1659
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.