RLG00000030298

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000105
Physical Location & Seq
Reverse (-)
59237031 .. 59242928
5898 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030298

Sequence Viewer

Length: 2871 bp
ATGGCGTCAACCTCTTCTCGTCGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGGGGGGAAGATACGCGCGATGGTTTTACCGCTTTTCTGTATTCTGCACTAACTCAGAGAGGAATTCTGACCTTTAAGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGGTGCAATTGAGGAGTCTAGATCTGCGATTGTCATTCTCTCATGGAGATATGCTTATTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCTTGAAAGAGAGGGGCCAACAAGTCATCCCCGTCTTCTTAAACGGCGTGGATCCTTCTGATGTGCGGCACCAAAGGGGATTTTTTGAGCTGAAAAGGAAACCCCAAATAGAGGTGGAAGTAAGGGAACATGAATTAGTTTATGGGGAGGATGAGGACAGACTAAATGCGTGGAGAGCTGCTTTGACTGAGGTCGCCAATCTTTCTGGCTGGGATTTCAAGAATGATTTATATCTTACAAAACTAATTGACGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGTGTTGATGATTTAATAGGCAATTACATATGTCCACAGCTTTGTGGGGTGTGTTTTATAGGGATCCATGGCATGCGGGGCGTAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTACAAGAAAAGCTTCTTTCCAAAATCCTGATGGCAAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATGGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCACCTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATCATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGTTAACGGGTTAAACGGTGGTGAAGCACTTCAACTTTTAAGTTTGAAGGCTTTCAAGAAATGTCCCCCACCAGAAGATTATTTGCATCTGTGCAACCGTATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATCTGGTAGAGGGGCTGATAAATGGGCAAGTGCAATAGATAGGCTAAGGAGCACACCAGATAAACGCATTATTGAAGTGCTTCGGATTAGTTTTGATGACCTGCATAAAACAGATAAAGAAATATTTCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTACAACCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTGATGAGCCAGGCAAACGTAGTAGATTATGGTCTCATAAAGACATCAACAAGGTGCTCGAGAGAAATAAGGGAACAGATTCAATCCAAGGCATGAATTTTGACGAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAGGCTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTGGTCTCATTGAATCTAACCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAGTTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATGAGGTAAATCTGGGGGAGATGGACTGTTTTGAGGAAACTGATGTGAATGGTGGGTCTGCAATAGAAATGTCATCTACTCTTGATCTCATAAAATCTGTGAGAGGTTCAATCTCTCATGGATGCGAAATAGTTTGGGGATCTTTAAATAAGTTATTGCCTTCTGGATTGGTGCCAAGAGTGAATACTGAGCCAATGAGTTTCTGCTTGCCTATATCTGGCCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACAAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTCTTCGTCTTCCTTCAGGCATTGGATTACTTTCTAAGCTTGAGAACTTGAACTTGGAGAATTGCAAGACACTTCAAGAGTTGTCAGACCTTCCATCAAATAACATACTAGATTTAAGGGCAGATGGTTGTACTTCGCTGAAATACTTTTTTGAGGTATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATTTAAGTGGCAATGAAGGATGCAATAACATAGCCTTAGAAATGCTGAAGACATTCTTGTCTCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTCTTTCAGTAGACCATTTTGCGGTGTCCAAATCCGATTGCTCTTTCTCTACCACCATTTATCATTTACTCTCTGAAAGGTTGGTGATTGAAGTTGACCACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

957

Amino Acids

107.4

Weight (kDa)

5.71

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 193 8.4e-46 TIR domain
TIR_2 PF13676 15 - 124 9.3e-13 TIR domain
NB-ARC PF00931 219 - 373 1.3e-17 NB-ARC domain
AAA_22 PF13401 223 - 320 3.8e-06 AAA domain
NACHT PF05729 225 - 342 1.9e-06 NACHT domain
WHD_ROQ1 PF23282 442 - 511 1e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 556 - 649 3.1e-07 Leucine-rich repeat region
LRR_14 PF23598 595 - 697 3.3e-09 Leucine-rich repeat region
LRR_13 PF23286 616 - 700 7.8e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2710
Acc36I ACCTGC 2 cut(s) 761, 1323
AccB1I GGYRCC 2 cut(s) 348, 2278
AccI GTMKAC 2 cut(s) 566, 2775
AccII CGCG 2 cut(s) 73, 75
AciI CCGC 5 cut(s) 87, 346, 685, 892, 2786
AclI AACGTT 1 cut(s) 1921
AclWI GGATC 7 cut(s) 326, 339, 667, 680, 942, 1386, 2254
AcsI RAATTY 6 cut(s) 120, 729, 1630, 1741, 2393, 2605
AcuI CTGAAG 2 cut(s) 2442, 2720
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 2575
AfiI CCNNNNNNNGG 4 cut(s) 391, 1424, 2324, 2785
AjnI CCWGG 4 cut(s) 985, 1543, 1992, 2758
AjuI GAANNNNNNNTTGG 2 cut(s) 2480, 2512
Alw21I GWGCWC 2 cut(s) 1268, 1593
Alw26I GTCTC 7 cut(s) 1536, 1572, 1676, 1820, 1940, 2002, 2718
AlwI GGATC 7 cut(s) 326, 339, 667, 680, 942, 1386, 2254
AlwNI CAGNNNCTG 1 cut(s) 875
Ama87I CYCGRG 4 cut(s) 711, 1592, 1817, 1898
AoxI GGCC 5 cut(s) 170, 295, 793, 1990, 2326
ApeKI GCWGC 2 cut(s) 458, 875
ApoI RAATTY 6 cut(s) 120, 729, 1630, 1741, 2393, 2605
AseI ATTAAT 1 cut(s) 1862
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 2 cut(s) 295, 983
AsuHPI GGTGA 5 cut(s) 929, 1091, 2384, 2428, 2860
AvaI CYCGRG 4 cut(s) 711, 1592, 1817, 1898
AvaII GGWCC 1 cut(s) 983
BamHI GGATCC 2 cut(s) 331, 672
BanI GGYRCC 2 cut(s) 348, 2278
BauI CACGAG 1 cut(s) 1193
BbsI GAAGAC 4 cut(s) 307, 2027, 2444, 2708
Bbv12I GWGCWC 2 cut(s) 1268, 1593
BbvI GCAGC 2 cut(s) 445, 862
BccI CCATC 8 cut(s) 71, 829, 877, 1764, 2054, 2122, 2545, 2561
BceAI ACGGC 2 cut(s) 340, 1997
BcgI CGANNNNNNTGC 2 cut(s) 1798, 1832
BciT130I CCWGG 4 cut(s) 987, 1545, 1994, 2760
BcoDI GTCTC 7 cut(s) 1536, 1572, 1676, 1820, 1940, 2002, 2718
BfaI CTAG 4 cut(s) 201, 797, 1397, 2552
BfmI CTRYAG 1 cut(s) 2017
BfuAI ACCTGC 2 cut(s) 761, 1323
BglII AGATCT 3 cut(s) 203, 1448, 1723
BisI GCNGC 3 cut(s) 347, 459, 876
BlsI GCNGC 3 cut(s) 348, 460, 877
Bme1390I CCNGG 4 cut(s) 987, 1545, 1994, 2760
Bme18I GGWCC 1 cut(s) 983
BmeT110I CYCGRG 4 cut(s) 711, 1592, 1817, 1898
BmgT120I GGNCC 2 cut(s) 295, 983
BmiI GGNNCC 5 cut(s) 296, 333, 350, 674, 2280
BmrFI CCNGG 4 cut(s) 987, 1545, 1994, 2760
BmsI GCATC 6 cut(s) 1036, 1153, 1473, 2078, 2219, 2663
BoxI GACNNNNGTC 1 cut(s) 470
BpiI GAAGAC 4 cut(s) 307, 2027, 2444, 2708
Bpu10I CCTNAGC 2 cut(s) 1173, 1259
BpuEI CTTGAG 3 cut(s) 170, 1733, 2504
BsaBI GATNNNNATC 3 cut(s) 510, 933, 1377
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 3 cut(s) 1572, 1676, 2002
BsaJI CCNNGG 4 cut(s) 676, 986, 1621, 1765
Bsc4I CCNNNNNNNGG 4 cut(s) 391, 1424, 2324, 2785
Bse1I ACTGG 2 cut(s) 980, 1993
Bse3DI GCAATG 1 cut(s) 2671
Bse8I GATNNNNATC 3 cut(s) 510, 933, 1377
BseBI CCWGG 4 cut(s) 987, 1545, 1994, 2760
BseDI CCNNGG 4 cut(s) 676, 986, 1621, 1765
BseGI GGATG 9 cut(s) 142, 262, 306, 436, 865, 1739, 2093, 2234, 2678
BseJI GATNNNNATC 3 cut(s) 510, 933, 1377
BseLI CCNNNNNNNGG 4 cut(s) 391, 1424, 2324, 2785
BseMI GCAATG 1 cut(s) 2671
BseMII CTCAG 6 cut(s) 125, 459, 1187, 2286, 2345, 2729
BseNI ACTGG 2 cut(s) 980, 1993
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 2 cut(s) 445, 862
BseYI CCCAGC 2 cut(s) 489, 1509
BsgI GTGCAG 1 cut(s) 87
Bsh1236I CGCG 2 cut(s) 73, 75
BshFI GGCC 5 cut(s) 172, 297, 795, 1992, 2328
BshNI GGYRCC 2 cut(s) 348, 2278
BsiHKAI GWGCWC 2 cut(s) 1268, 1593
BsiHKCI CYCGRG 4 cut(s) 711, 1592, 1817, 1898
BslFI GGGAC 1 cut(s) 1107
BslI CCNNNNNNNGG 4 cut(s) 391, 1424, 2324, 2785
BsmAI GTCTC 7 cut(s) 1536, 1572, 1676, 1820, 1940, 2002, 2718
BsmFI GGGAC 1 cut(s) 1107
BsnI GGCC 5 cut(s) 172, 297, 795, 1992, 2328
Bso31I GGTCTC 3 cut(s) 1572, 1676, 2002
BsoBI CYCGRG 4 cut(s) 711, 1592, 1817, 1898
Bsp1286I GDGCHC 2 cut(s) 1268, 1593
Bsp143I GATC 9 cut(s) 203, 331, 672, 934, 1378, 1448, 1723, 2191, 2246
Bsp19I CCATGG 1 cut(s) 676
BspACI CCGC 5 cut(s) 87, 346, 685, 892, 2786
BspANI GGCC 5 cut(s) 172, 297, 795, 1992, 2328
BspCNI CTCAG 6 cut(s) 124, 460, 1186, 2287, 2346, 2728
BspFNI CGCG 2 cut(s) 73, 75
BspLI GGNNCC 5 cut(s) 296, 333, 350, 674, 2280
BspMAI CTGCAG 1 cut(s) 2021
BspMI ACCTGC 2 cut(s) 761, 1323
BspPI GGATC 7 cut(s) 326, 339, 667, 680, 942, 1386, 2254
BspT107I GGYRCC 2 cut(s) 348, 2278
BspTNI GGTCTC 3 cut(s) 1572, 1676, 2002
BsrDI GCAATG 1 cut(s) 2671
BsrI ACTGG 2 cut(s) 980, 1993
BssECI CCNNGG 4 cut(s) 676, 986, 1621, 1765
BssMI GATC 9 cut(s) 203, 331, 672, 934, 1378, 1448, 1723, 2191, 2246
BssNAI GTATAC 1 cut(s) 567
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1193
BssT1I CCWWGG 3 cut(s) 676, 1621, 1765
Bst1107I GTATAC 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 1193
Bst2UI CCWGG 4 cut(s) 987, 1545, 1994, 2760
Bst4CI ACNGT 5 cut(s) 1076, 1157, 1479, 1530, 2135
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1492
BstC8I GCNNGC 5 cut(s) 683, 1354, 1409, 1699, 2315
BstDSI CCRYGG 1 cut(s) 676
BstEII GGTNACC 1 cut(s) 2416
BstF5I GGATG 9 cut(s) 142, 262, 306, 436, 865, 1739, 2093, 2234, 2678
BstFNI CGCG 2 cut(s) 73, 75
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 9 cut(s) 206, 334, 675, 937, 1381, 1451, 1726, 2194, 2249
BstMAI GTCTC 7 cut(s) 1536, 1572, 1676, 1820, 1940, 2002, 2718
BstMBI GATC 9 cut(s) 203, 331, 672, 934, 1378, 1448, 1723, 2191, 2246
BstMWI GCNNNNNNNGC 3 cut(s) 455, 687, 1492
BstNI CCWGG 4 cut(s) 987, 1545, 1994, 2760
BstNSI RCATGY 1 cut(s) 685
BstPAI GACNNNNGTC 1 cut(s) 470
BstPI GGTNACC 1 cut(s) 2416
BstSCI CCNGG 4 cut(s) 985, 1543, 1992, 2758
BstSFI CTRYAG 1 cut(s) 2017
BstUI CGCG 2 cut(s) 73, 75
BstV1I GCAGC 2 cut(s) 445, 862
BstV2I GAAGAC 4 cut(s) 307, 2027, 2444, 2708
BstX2I RGATCY 6 cut(s) 203, 331, 672, 1448, 1723, 2246
BstYI RGATCY 6 cut(s) 203, 331, 672, 1448, 1723, 2246
BstZ17I GTATAC 1 cut(s) 567
BsuRI GGCC 5 cut(s) 172, 297, 795, 1992, 2328
BtgI CCRYGG 1 cut(s) 676
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 9 cut(s) 142, 262, 306, 436, 865, 1739, 2093, 2234, 2678
BveI ACCTGC 2 cut(s) 761, 1323
Cac8I GCNNGC 5 cut(s) 683, 1354, 1409, 1699, 2315
CaiI CAGNNNCTG 1 cut(s) 875
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 295, 983
CseI GACGC 1 cut(s) 149
Csp6I GTAC 1 cut(s) 2574
CspCI CAANNNNNGTGG 2 cut(s) 2641, 2676
CviQI GTAC 1 cut(s) 2574
DpnI GATC 9 cut(s) 205, 333, 674, 936, 1380, 1450, 1725, 2193, 2248
DpnII GATC 9 cut(s) 203, 331, 672, 934, 1378, 1448, 1723, 2191, 2246
DraI TTTAAA 1 cut(s) 2253
DrdI GACNNNNNNGTC 1 cut(s) 2710
DseDI GACNNNNNNGTC 1 cut(s) 2710
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 3 cut(s) 676, 1621, 1765
Eco147I AGGCCT 1 cut(s) 172
Eco31I GGTCTC 3 cut(s) 1572, 1676, 2002
Eco47I GGWCC 1 cut(s) 983
Eco57I CTGAAG 2 cut(s) 2442, 2720
Eco88I CYCGRG 4 cut(s) 711, 1592, 1817, 1898
Eco91I GGTNACC 1 cut(s) 2416
EcoO65I GGTNACC 1 cut(s) 2416
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 4 cut(s) 985, 1543, 1992, 2758
EcoT14I CCWWGG 3 cut(s) 676, 1621, 1765
EcoT22I ATGCAT 1 cut(s) 1488
ErhI CCWWGG 3 cut(s) 676, 1621, 1765
FalI AAGNNNNNCTT 4 cut(s) 801, 833, 2693, 2725
FaqI GGGAC 1 cut(s) 1107
FauI CCCGC 1 cut(s) 678
FauNDI CATATG 1 cut(s) 638
FblI GTMKAC 2 cut(s) 566, 2775
Fnu4HI GCNGC 3 cut(s) 347, 459, 876
FokI GGATG 9 cut(s) 149, 269, 293, 443, 872, 1746, 2100, 2241, 2685
Fsp4HI GCNGC 3 cut(s) 347, 459, 876
FspBI CTAG 4 cut(s) 201, 797, 1397, 2552
GlaI GCGC 1 cut(s) 74
GluI GCNGC 3 cut(s) 347, 459, 876
GsaI CCCAGC 2 cut(s) 493, 1513
HaeIII GGCC 5 cut(s) 172, 297, 795, 1992, 2328
HgaI GACGC 1 cut(s) 149
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 4 cut(s) 9, 583, 1063, 2860
HindII GTYRAC 4 cut(s) 9, 583, 1063, 2860
HindIII AAGCTT 3 cut(s) 815, 1781, 2480
HpaI GTTAAC 1 cut(s) 1063
HphI GGTGA 5 cut(s) 929, 1091, 2384, 2428, 2860
Hpy166II GTNNAC 8 cut(s) 9, 567, 583, 644, 1063, 1526, 2776, 2860
Hpy188I TCNGA 9 cut(s) 114, 126, 340, 1299, 1707, 2349, 2530, 2800, 2839
Hpy8I GTNNAC 8 cut(s) 9, 567, 583, 644, 1063, 1526, 2776, 2860
Hpy99I CGWCG 2 cut(s) 24, 27
HpyAV CCTTC 9 cut(s) 345, 1099, 1363, 1724, 2277, 2369, 2466, 2543, 2663
HpyCH4III ACNGT 5 cut(s) 1076, 1157, 1479, 1530, 2135
HpyCH4IV ACGT 2 cut(s) 1552, 1921
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 687, 1492
HpySE526I ACGT 2 cut(s) 1552, 1921
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 73
KspAI GTTAAC 1 cut(s) 1063
Kzo9I GATC 9 cut(s) 203, 331, 672, 934, 1378, 1448, 1723, 2191, 2246
LmnI GCTCC 3 cut(s) 872, 1263, 2378
Lsp1109I GCAGC 2 cut(s) 445, 862
LweI GCATC 6 cut(s) 1036, 1153, 1473, 2078, 2219, 2663
MaeI CTAG 4 cut(s) 201, 797, 1397, 2552
MaeII ACGT 2 cut(s) 1552, 1921
MaeIII GTNAC 3 cut(s) 1384, 2399, 2416
MalI GATC 9 cut(s) 205, 333, 674, 936, 1380, 1450, 1725, 2193, 2248
MboI GATC 9 cut(s) 203, 331, 672, 934, 1378, 1448, 1723, 2191, 2246
MfeI CAATTG 3 cut(s) 189, 1974, 2644
MflI RGATCY 6 cut(s) 203, 331, 672, 1448, 1723, 2246
MhlI GDGCHC 2 cut(s) 1268, 1593
MlyI GAGTC 2 cut(s) 206, 1831
MmeI TCCRAC 2 cut(s) 1491, 1888
Mph1103I ATGCAT 1 cut(s) 1488
MslI CAYNNNNRTG 1 cut(s) 599
MspR9I CCNGG 4 cut(s) 987, 1545, 1994, 2760
MunI CAATTG 3 cut(s) 189, 1974, 2644
MvaI CCWGG 4 cut(s) 987, 1545, 1994, 2760
MvnI CGCG 2 cut(s) 73, 75
MwoI GCNNNNNNNGC 3 cut(s) 455, 687, 1492
NcoI CCATGG 1 cut(s) 676
NdeI CATATG 1 cut(s) 638
NdeII GATC 9 cut(s) 203, 331, 672, 934, 1378, 1448, 1723, 2191, 2246
NlaIV GGNNCC 5 cut(s) 296, 333, 350, 674, 2280
NmeAIII GCCGAG 1 cut(s) 2079
NmuCI GTSAC 2 cut(s) 1384, 2416
NsiI ATGCAT 1 cut(s) 1488
NspI RCATGY 1 cut(s) 685
PaeI GCATGC 1 cut(s) 685
PaeR7I CTCGAG 3 cut(s) 711, 1592, 1817
PceI AGGCCT 1 cut(s) 172
PfoI TCCNGGA 1 cut(s) 2758
PkrI GCNGC 3 cut(s) 348, 460, 877
PleI GAGTC 2 cut(s) 205, 1830
PpsI GAGTC 2 cut(s) 205, 1830
PshAI GACNNNNGTC 1 cut(s) 470
PshBI ATTAAT 1 cut(s) 1862
Psp1406I AACGTT 1 cut(s) 1921
Psp6I CCWGG 4 cut(s) 985, 1543, 1992, 2758
PspEI GGTNACC 1 cut(s) 2416
PspFI CCCAGC 2 cut(s) 489, 1509
PspGI CCWGG 4 cut(s) 985, 1543, 1992, 2758
PspN4I GGNNCC 5 cut(s) 296, 333, 350, 674, 2280
PspPI GGNCC 2 cut(s) 295, 983
PspXI VCTCGAGB 1 cut(s) 711
PstI CTGCAG 1 cut(s) 2021
PstNI CAGNNNCTG 1 cut(s) 875
PsuI RGATCY 6 cut(s) 203, 331, 672, 1448, 1723, 2246
RsaI GTAC 1 cut(s) 2575
RsaNI GTAC 1 cut(s) 2574
RseI CAYNNNNRTG 1 cut(s) 599
SatI GCNGC 3 cut(s) 347, 459, 876
Sau3AI GATC 9 cut(s) 203, 331, 672, 934, 1378, 1448, 1723, 2191, 2246
Sau96I GGNCC 2 cut(s) 295, 983
SchI GAGTC 2 cut(s) 206, 1831
ScrFI CCNGG 4 cut(s) 987, 1545, 1994, 2760
SduI GDGCHC 2 cut(s) 1268, 1593
SfaNI GCATC 6 cut(s) 1036, 1153, 1473, 2078, 2219, 2663
SfcI CTRYAG 1 cut(s) 2017
Sfr274I CTCGAG 3 cut(s) 711, 1592, 1817
SinI GGWCC 1 cut(s) 983
SlaI CTCGAG 3 cut(s) 711, 1592, 1817
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 6 cut(s) 149, 711, 1592, 1712, 1817, 2483
SmoI CTYRAG 6 cut(s) 149, 711, 1592, 1712, 1817, 2483
SphI GCATGC 1 cut(s) 685
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 5 cut(s) 87, 346, 685, 892, 2786
SspI AATATT 1 cut(s) 1338
SspMI CTAG 4 cut(s) 201, 797, 1397, 2552
StuI AGGCCT 1 cut(s) 172
StyD4I CCNGG 4 cut(s) 985, 1543, 1992, 2758
StyI CCWWGG 3 cut(s) 676, 1621, 1765
TaaI ACNGT 5 cut(s) 1076, 1157, 1479, 1530, 2135
TaiI ACGT 2 cut(s) 1555, 1924
TaqI TCGA 4 cut(s) 712, 1481, 1593, 1818
TaqII GACCGA 1 cut(s) 762
TatI WGTACW 1 cut(s) 2573
TauI GCSGC 1 cut(s) 349
TseFI GTSAC 2 cut(s) 1384, 2416
TseI GCWGC 2 cut(s) 458, 875
Tsp45I GTSAC 2 cut(s) 1384, 2416
TspDTI ATGAA 9 cut(s) 273, 426, 583, 741, 876, 1639, 1643, 2629, 2682
TspGWI ACGGA 2 cut(s) 550, 1173
VpaK11BI GGWCC 1 cut(s) 983
VspI ATTAAT 1 cut(s) 1862
XapI RAATTY 6 cut(s) 120, 729, 1630, 1741, 2393, 2605
XbaI TCTAGA 1 cut(s) 200
XceI RCATGY 1 cut(s) 685
XcmI CCANNNNNNNNNTGG 2 cut(s) 832, 1763
XhoI CTCGAG 3 cut(s) 711, 1592, 1817
XmiI GTMKAC 2 cut(s) 566, 2775
XspI CTAG 4 cut(s) 201, 797, 1397, 2552
Zsp2I ATGCAT 1 cut(s) 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.