Rw1G004390

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
8376877 .. 8381055
4179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004390.1

Sequence Viewer

Length: 3576 bp
ATGACTAGAAAGTGGAAATACGATGTCTTTCTAAGTTTCAGCGGGGCAGATACCCGCTATACTTTTACCAATCGTTTGTATTCTGCACTAATTCGAAAAGGGATTCTGACCTTTATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGGTGCAATTGAGGACTCTAGATCTGCCATTGTCATTCTCTCAAAGAAGTATGCTGCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGAGGGGCCAACAAGTCTTCCCCGTCTTCTACTTGGGCGTGGATCCTTCTGATGTGCGGCACCAAAGGCGAAGTTTTGAGCTCATCAAATGGGAACACCAAGTAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGGCAAGAATCATGATAGACTAAATGCGTGGAGAGCTGCTTTGACTGAAGTGGCCAATCTGTCTGGCTGGGATAACAAGAATTATGGATATGAACAAATACTAATTGAGGAAATTGTTAATGATATACTAAAGAAGTCCGTATATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGGTTCCCGTATTGATAGTTTATTAAGCAAATACATATATCCGCAGCTTGATGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCCAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCTAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTCGTGTAGAATGAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGATACAATTAGAAAAATTGGCTGGAAATCGCAACTGGTTTGGTCCGGGGAGTAGAATTATCATAACCACCACAGATATTCAGTTGTTAAAGGCGCATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATTTCCCCCACCAGAAGATTATTTGCATCTGTGCTACCGTATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTTGTGGTTTTAGGTTCGTTTTTATTTGGTAGAAGAACTGATCAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATTATGATCGTGTGATACAAATACTAGACTATTGCCAACTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAATGAACTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCCAAAGAGCCAGGCAAACGTAGTAGACTATGGTCTCATGAAGACATCAACAATGTGCTGAAAAAAAATATGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAATCCAGAAGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCATAATGTGGACCTTCCCGAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCAAAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGACAGGTTGAAGTTCATCAAACTCTGTCATTCTCAAAATATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAAAGTTTAGATCTTGAAGGATGTGAGAATTTGGTTAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAATCTCAAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAATGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGTGTCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTGAGCAAATGCAGAAATCTTGTCCGTCTTCCGAGCACCATCAATAAATTGAAGTCTATTAAAAATCTTGATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGAGATATATTGTTTTGAGGAAAATGATGTGAATAGTAGGTCTGCAATAGAAATGTCATCTTCCCATGATCTCATAAAATCTGTGAGAGGTTCAATCTTTCATGTATATGAAGTAGTTTGGGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATGCAGGGCCATCGAGTTTCCGCTTGCCTATTTCTGGTTTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTCCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACCATTTTGTTCGCGTTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTATCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAATAGACTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAAGAAAGGATGCAATAATATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCCAAGTGGTTCGGCCATCAGAGTGTTGGGTGTTCATTAAGTGTACCTCTACCTGCAGATTGGAATAACAGTCGGTTTTTAGGATTTGCTTTGTGTGCTGATTTTGTACTCCGTGAGCACCATCGGGTGGATGAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGATCGATGGAAGAGAACTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAATCAAATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGCTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATACGAGCAAGAAGTGCAAGGCCTGAACCAAACAACCACTCAATCAAGCAGTAGGATGTCTCCTTATGCAGACATATTGATTGGTTTTGACATTCCAGGTGGAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACACTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1191

Amino Acids

135.68

Weight (kDa)

6.51

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 6 - 188 3.1e-45 TIR domain
TIR_2 PF13676 9 - 106 5.8e-15 TIR domain
NB-ARC PF00931 213 - 368 2.2e-17 NB-ARC domain
NACHT PF05729 218 - 312 1.8e-06 NACHT domain
WHD_ROQ1 PF23282 435 - 506 4.1e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 695 - 779 1.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 706 - 774 1.6e-09 Leucine-rich repeat region
C-JID PF20160 998 - 1147 3.9e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 746, 3070
AccB1I GGYRCC 1 cut(s) 330
AccB7I CCANNNNNTGG 2 cut(s) 1612, 3136
AccI GTMKAC 2 cut(s) 1543, 2785
AccII CGCG 1 cut(s) 2688
AciI CCGC 7 cut(s) 42, 55, 328, 629, 670, 877, 2548
AclWI GGATC 6 cut(s) 308, 321, 652, 665, 927, 2491
AcoI YGGCCR 2 cut(s) 459, 3022
AcuI CTGAAG 5 cut(s) 474, 2679, 2957, 3221, 3413
AfaI GTAC 4 cut(s) 2812, 3054, 3117, 3328
AfiI CCNNNNNNNGG 5 cut(s) 1409, 1612, 2561, 3136, 3524
AflII CTTAAG 1 cut(s) 1774
AjnI CCWGG 4 cut(s) 1528, 2995, 3270, 3517
AjuI GAANNNNNNNTTGG 2 cut(s) 2717, 2749
Alw21I GWGCWC 3 cut(s) 354, 2282, 3129
Alw26I GTCTC 8 cut(s) 1521, 1557, 1680, 1741, 1913, 2177, 2743, 3486
AlwI GGATC 6 cut(s) 308, 321, 652, 665, 927, 2491
AlwNI CAGNNNCTG 1 cut(s) 860
Ama87I CYCGRG 2 cut(s) 696, 2135
ApeKI GCWGC 4 cut(s) 218, 443, 631, 860
AseI ATTAAT 1 cut(s) 2099
Asp700I GAANNNNTTC 7 cut(s) 589, 801, 1071, 1503, 1597, 2942, 2979
AspLEI GCGC 1 cut(s) 1021
AspS9I GGNCC 5 cut(s) 277, 968, 1708, 2534, 3383
AsuC2I CCSGG 2 cut(s) 972, 3387
AsuHPI GGTGA 4 cut(s) 2621, 2665, 3278, 3409
AsuII TTCGAA 1 cut(s) 94
AvaI CYCGRG 2 cut(s) 696, 2135
AvaII GGWCC 2 cut(s) 968, 1708
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 2 cut(s) 313, 657
BanI GGYRCC 1 cut(s) 330
BanII GRGCYC 1 cut(s) 354
BauI CACGAG 1 cut(s) 3309
BbsI GAAGAC 6 cut(s) 280, 289, 1566, 1679, 2264, 2945
Bbv12I GWGCWC 3 cut(s) 354, 2282, 3129
BbvI GCAGC 4 cut(s) 205, 430, 643, 847
BceAI ACGGC 1 cut(s) 2234
BcgI CGANNNNNNTGC 2 cut(s) 2520, 2554
BciT130I CCWGG 4 cut(s) 1530, 2997, 3272, 3519
BciVI GTATCC 1 cut(s) 1779
BclI TGATCA 1 cut(s) 1216
BcnI CCSGG 2 cut(s) 972, 3387
BcoDI GTCTC 8 cut(s) 1521, 1557, 1680, 1741, 1913, 2177, 2743, 3486
BfaI CTAG 5 cut(s) 6, 183, 782, 1382, 2789
BfmI CTRYAG 1 cut(s) 3063
BfrI CTTAAG 1 cut(s) 1774
BfuAI ACCTGC 2 cut(s) 746, 3070
BfuI GTATCC 1 cut(s) 1779
BglII AGATCT 4 cut(s) 185, 1433, 1778, 1960
BisI GCNGC 5 cut(s) 219, 329, 444, 632, 861
BlsI GCNGC 5 cut(s) 220, 330, 445, 633, 862
Bme1390I CCNGG 6 cut(s) 972, 1530, 2997, 3272, 3387, 3519
Bme18I GGWCC 2 cut(s) 968, 1708
BmeT110I CYCGRG 2 cut(s) 696, 2135
BmgT120I GGNCC 5 cut(s) 277, 968, 1708, 2534, 3383
BmiI GGNNCC 5 cut(s) 278, 315, 332, 592, 659
BmrFI CCNGG 6 cut(s) 972, 1530, 2997, 3272, 3387, 3519
BmrI ACTGGG 1 cut(s) 1773
BmsI GCATC 7 cut(s) 1021, 1138, 1458, 2315, 2824, 2846, 2900
BmuI ACTGGG 1 cut(s) 1773
BoxI GACNNNNGTC 1 cut(s) 1549
BpiI GAAGAC 6 cut(s) 280, 289, 1566, 1679, 2264, 2945
Bpu10I CCTNAGC 1 cut(s) 1158
Bpu14I TTCGAA 1 cut(s) 94
BpuEI CTTGAG 5 cut(s) 152, 1765, 2039, 2741, 3380
BpuMI CCSGG 2 cut(s) 972, 3387
Bsa29I ATCGAT 1 cut(s) 3207
BsaBI GATNNNNATC 2 cut(s) 918, 1362
BsaI GGTCTC 2 cut(s) 1557, 1913
BsaJI CCNNGG 4 cut(s) 661, 971, 1606, 2002
Bsc4I CCNNNNNNNGG 5 cut(s) 1409, 1612, 2561, 3136, 3524
Bse1I ACTGG 6 cut(s) 965, 1305, 1768, 3225, 3534, 3547
Bse8I GATNNNNATC 2 cut(s) 918, 1362
BseBI CCWGG 4 cut(s) 1530, 2997, 3272, 3519
BseCI ATCGAT 1 cut(s) 3207
BseDI CCNNGG 4 cut(s) 661, 971, 1606, 2002
BseJI GATNNNNATC 2 cut(s) 918, 1362
BseLI CCNNNNNNNGG 5 cut(s) 1409, 1612, 2561, 3136, 3524
BseMII CTCAG 5 cut(s) 1172, 1696, 2213, 2237, 2582
BseNI ACTGG 6 cut(s) 965, 1305, 1768, 3225, 3534, 3547
BseRI GAGGAG 1 cut(s) 1742
BseXI GCAGC 4 cut(s) 205, 430, 643, 847
BseYI CCCAGC 2 cut(s) 474, 1494
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 2688
Bsh1285I CGRYCG 1 cut(s) 881
BshNI GGYRCC 1 cut(s) 330
BshVI ATCGAT 1 cut(s) 3207
BsiEI CGRYCG 1 cut(s) 881
BsiHKAI GWGCWC 3 cut(s) 354, 2282, 3129
BsiHKCI CYCGRG 2 cut(s) 696, 2135
BsiSI CCGG 2 cut(s) 971, 3386
BslI CCNNNNNNNGG 5 cut(s) 1409, 1612, 2561, 3136, 3524
BsmAI GTCTC 8 cut(s) 1521, 1557, 1680, 1741, 1913, 2177, 2743, 3486
BsmI GAATGC 2 cut(s) 2533, 2700
Bso31I GGTCTC 2 cut(s) 1557, 1913
BsoBI CYCGRG 2 cut(s) 696, 2135
Bsp119I TTCGAA 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 354, 2282, 3129
Bsp19I CCATGG 1 cut(s) 661
BspACI CCGC 7 cut(s) 42, 55, 328, 629, 670, 877, 2548
BspCNI CTCAG 5 cut(s) 1171, 1695, 2214, 2238, 2583
BspDI ATCGAT 1 cut(s) 3207
BspFNI CGCG 1 cut(s) 2688
BspHI TCATGA 2 cut(s) 418, 1555
BspLI GGNNCC 5 cut(s) 278, 315, 332, 592, 659
BspMAI CTGCAG 1 cut(s) 3067
BspMI ACCTGC 2 cut(s) 746, 3070
BspPI GGATC 6 cut(s) 308, 321, 652, 665, 927, 2491
BspT104I TTCGAA 1 cut(s) 94
BspT107I GGYRCC 1 cut(s) 330
BspTI CTTAAG 1 cut(s) 1774
BspTNI GGTCTC 2 cut(s) 1557, 1913
BsrI ACTGG 6 cut(s) 965, 1305, 1768, 3225, 3534, 3547
BssECI CCNNGG 4 cut(s) 661, 971, 1606, 2002
BssSI CACGAG 1 cut(s) 3309
BssT1I CCWWGG 3 cut(s) 661, 1606, 2002
Bst2BI CACGAG 1 cut(s) 3309
Bst2UI CCWGG 4 cut(s) 1530, 2997, 3272, 3519
Bst4CI ACNGT 6 cut(s) 1061, 1142, 1464, 1515, 3080, 3347
Bst6I CTCTTC 1 cut(s) 3208
BstAFI CTTAAG 1 cut(s) 1774
BstAPI GCANNNNNTGC 3 cut(s) 1477, 3436, 3555
BstBI TTCGAA 1 cut(s) 94
BstC8I GCNNGC 5 cut(s) 668, 1339, 1936, 2552, 3243
BstDEI CTNAG 8 cut(s) 32, 1158, 1682, 2222, 2246, 2591, 2666, 2715
BstDSI CCRYGG 1 cut(s) 661
BstEII GGTNACC 1 cut(s) 2653
BstFNI CGCG 1 cut(s) 2688
BstHHI GCGC 1 cut(s) 1021
BstMAI GTCTC 8 cut(s) 1521, 1557, 1680, 1741, 1913, 2177, 2743, 3486
BstMCI CGRYCG 1 cut(s) 881
BstMWI GCNNNNNNNGC 9 cut(s) 337, 440, 672, 1477, 2716, 3104, 3436, 3546, 3555
BstNI CCWGG 4 cut(s) 1530, 2997, 3272, 3519
BstNSI RCATGY 1 cut(s) 670
BstPAI GACNNNNGTC 1 cut(s) 1549
BstPI GGTNACC 1 cut(s) 2653
BstSCI CCNGG 6 cut(s) 970, 1528, 2995, 3270, 3385, 3517
BstSFI CTRYAG 1 cut(s) 3063
BstUI CGCG 1 cut(s) 2688
BstV1I GCAGC 4 cut(s) 205, 430, 643, 847
BstV2I GAAGAC 6 cut(s) 280, 289, 1566, 1679, 2264, 2945
BstX2I RGATCY 7 cut(s) 185, 313, 657, 1433, 1778, 1960, 2483
BstYI RGATCY 7 cut(s) 185, 313, 657, 1433, 1778, 1960, 2483
Bsu15I ATCGAT 1 cut(s) 3207
BsuI GTATCC 1 cut(s) 1779
BsuTUI ATCGAT 1 cut(s) 3207
BtgI CCRYGG 1 cut(s) 661
BtsIMutI CAGTG 3 cut(s) 2816, 3540, 3541
BveI ACCTGC 2 cut(s) 746, 3070
Cac8I GCNNGC 5 cut(s) 668, 1339, 1936, 2552, 3243
CaiI CAGNNNCTG 1 cut(s) 860
CciI TCATGA 2 cut(s) 418, 1555
CfoI GCGC 1 cut(s) 1021
Cfr13I GGNCC 5 cut(s) 277, 968, 1708, 2534, 3383
ClaI ATCGAT 1 cut(s) 3207
CseI GACGC 1 cut(s) 131
Csp6I GTAC 4 cut(s) 2811, 3053, 3116, 3327
CviQI GTAC 4 cut(s) 2811, 3053, 3116, 3327
DdeI CTNAG 8 cut(s) 32, 1158, 1682, 2222, 2246, 2591, 2666, 2715
DraI TTTAAA 1 cut(s) 2490
EaeI YGGCCR 2 cut(s) 459, 3022
Eam1104I CTCTTC 1 cut(s) 3208
EarI CTCTTC 1 cut(s) 3208
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 3 cut(s) 661, 1606, 2002
Eco147I AGGCCT 3 cut(s) 154, 1722, 3444
Eco24I GRGCYC 1 cut(s) 354
Eco31I GGTCTC 2 cut(s) 1557, 1913
Eco47I GGWCC 2 cut(s) 968, 1708
Eco53kI GAGCTC 1 cut(s) 352
Eco57I CTGAAG 5 cut(s) 474, 2679, 2957, 3221, 3413
Eco88I CYCGRG 2 cut(s) 696, 2135
Eco91I GGTNACC 1 cut(s) 2653
EcoICRI GAGCTC 1 cut(s) 352
EcoO65I GGTNACC 1 cut(s) 2653
EcoRI GAATTC 1 cut(s) 3158
EcoRII CCWGG 4 cut(s) 1528, 2995, 3270, 3517
EcoT14I CCWWGG 3 cut(s) 661, 1606, 2002
EcoT22I ATGCAT 3 cut(s) 266, 1473, 2839
EcoT38I GRGCYC 1 cut(s) 354
ErhI CCWWGG 3 cut(s) 661, 1606, 2002
FalI AAGNNNNNCTT 2 cut(s) 786, 818
FauI CCCGC 3 cut(s) 35, 62, 663
FbaI TGATCA 1 cut(s) 1216
FblI GTMKAC 2 cut(s) 1543, 2785
Fnu4HI GCNGC 5 cut(s) 219, 329, 444, 632, 861
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 5 cut(s) 219, 329, 444, 632, 861
FspBI CTAG 5 cut(s) 6, 183, 782, 1382, 2789
GlaI GCGC 1 cut(s) 1020
GluI GCNGC 5 cut(s) 219, 329, 444, 632, 861
GsaI CCCAGC 2 cut(s) 478, 1498
HapII CCGG 2 cut(s) 971, 3386
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 1021
Hin6I GCGC 1 cut(s) 1019
HinP1I GCGC 1 cut(s) 1019
HincII GTYRAC 2 cut(s) 568, 1626
HindII GTYRAC 2 cut(s) 568, 1626
HindIII AAGCTT 2 cut(s) 2018, 2717
HpaII CCGG 2 cut(s) 971, 3386
HphI GGTGA 4 cut(s) 2621, 2665, 3278, 3409
Hpy166II GTNNAC 7 cut(s) 568, 1511, 1544, 1626, 1708, 2786, 3053
Hpy8I GTNNAC 7 cut(s) 568, 1511, 1544, 1626, 1708, 2786, 3053
HpyCH4III ACNGT 6 cut(s) 1061, 1142, 1464, 1515, 3080, 3347
HpyCH4IV ACGT 2 cut(s) 1537, 2579
HpyF10VI GCNNNNNNNGC 9 cut(s) 337, 440, 672, 1477, 2716, 3104, 3436, 3546, 3555
HpyF3I CTNAG 8 cut(s) 32, 1158, 1682, 2222, 2246, 2591, 2666, 2715
HpySE526I ACGT 2 cut(s) 1537, 2579
HspAI GCGC 1 cut(s) 1019
Ksp22I TGATCA 1 cut(s) 1216
LmnI GCTCC 2 cut(s) 857, 2615
Lsp1109I GCAGC 4 cut(s) 205, 430, 643, 847
LweI GCATC 7 cut(s) 1021, 1138, 1458, 2315, 2824, 2846, 2900
MaeI CTAG 5 cut(s) 6, 183, 782, 1382, 2789
MaeII ACGT 2 cut(s) 1537, 2579
MaeIII GTNAC 2 cut(s) 2636, 2653
MfeI CAATTG 3 cut(s) 171, 2211, 2881
MflI RGATCY 7 cut(s) 185, 313, 657, 1433, 1778, 1960, 2483
MhlI GDGCHC 3 cut(s) 354, 2282, 3129
MlsI TGGCCA 1 cut(s) 461
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 4 cut(s) 173, 1743, 2068, 3420
MmeI TCCRAC 2 cut(s) 1476, 2125
Mox20I TGGCCA 1 cut(s) 461
Mph1103I ATGCAT 3 cut(s) 266, 1473, 2839
MroXI GAANNNNTTC 7 cut(s) 589, 801, 1071, 1503, 1597, 2942, 2979
MscI TGGCCA 1 cut(s) 461
MslI CAYNNNNRTG 4 cut(s) 584, 1616, 2466, 3030
Msp20I TGGCCA 1 cut(s) 461
MspA1I CMGCKG 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 1774
MspI CCGG 2 cut(s) 971, 3386
MspR9I CCNGG 6 cut(s) 972, 1530, 2997, 3272, 3387, 3519
MunI CAATTG 3 cut(s) 171, 2211, 2881
Mva1269I GAATGC 2 cut(s) 2533, 2700
MvaI CCWGG 4 cut(s) 1530, 2997, 3272, 3519
MvnI CGCG 1 cut(s) 2688
MwoI GCNNNNNNNGC 9 cut(s) 337, 440, 672, 1477, 2716, 3104, 3436, 3546, 3555
NciI CCSGG 2 cut(s) 972, 3387
NcoI CCATGG 1 cut(s) 661
NlaIV GGNNCC 5 cut(s) 278, 315, 332, 592, 659
NmeAIII GCCGAG 1 cut(s) 2316
NmuCI GTSAC 1 cut(s) 2653
NsiI ATGCAT 3 cut(s) 266, 1473, 2839
NspI RCATGY 1 cut(s) 670
NspV TTCGAA 1 cut(s) 94
PaeI GCATGC 1 cut(s) 670
PaeR7I CTCGAG 1 cut(s) 696
PagI TCATGA 2 cut(s) 418, 1555
PceI AGGCCT 3 cut(s) 154, 1722, 3444
PctI GAATGC 2 cut(s) 2533, 2700
PdmI GAANNNNTTC 7 cut(s) 589, 801, 1071, 1503, 1597, 2942, 2979
PflMI CCANNNNNTGG 2 cut(s) 1612, 3136
PfoI TCCNGGA 1 cut(s) 2995
PkrI GCNGC 5 cut(s) 220, 330, 445, 633, 862
PleI GAGTC 4 cut(s) 173, 1743, 2067, 3419
PpsI GAGTC 4 cut(s) 173, 1743, 2067, 3419
PshAI GACNNNNGTC 1 cut(s) 1549
PshBI ATTAAT 1 cut(s) 2099
Psp124BI GAGCTC 1 cut(s) 354
Psp6I CCWGG 4 cut(s) 1528, 2995, 3270, 3517
PspEI GGTNACC 1 cut(s) 2653
PspFI CCCAGC 2 cut(s) 474, 1494
PspGI CCWGG 4 cut(s) 1528, 2995, 3270, 3517
PspN4I GGNNCC 5 cut(s) 278, 315, 332, 592, 659
PspPI GGNCC 5 cut(s) 277, 968, 1708, 2534, 3383
PspXI VCTCGAGB 1 cut(s) 696
PstI CTGCAG 1 cut(s) 3067
PstNI CAGNNNCTG 1 cut(s) 860
PsuI RGATCY 7 cut(s) 185, 313, 657, 1433, 1778, 1960, 2483
RsaI GTAC 4 cut(s) 2812, 3054, 3117, 3328
RsaNI GTAC 4 cut(s) 2811, 3053, 3116, 3327
RseI CAYNNNNRTG 4 cut(s) 584, 1616, 2466, 3030
SacI GAGCTC 1 cut(s) 354
SatI GCNGC 5 cut(s) 219, 329, 444, 632, 861
Sau96I GGNCC 5 cut(s) 277, 968, 1708, 2534, 3383
SchI GAGTC 4 cut(s) 173, 1743, 2068, 3420
ScrFI CCNGG 6 cut(s) 972, 1530, 2997, 3272, 3387, 3519
SduI GDGCHC 3 cut(s) 354, 2282, 3129
SfaNI GCATC 7 cut(s) 1021, 1138, 1458, 2315, 2824, 2846, 2900
SfcI CTRYAG 1 cut(s) 3063
Sfr274I CTCGAG 1 cut(s) 696
SfuI TTCGAA 1 cut(s) 94
SinI GGWCC 2 cut(s) 968, 1708
SlaI CTCGAG 1 cut(s) 696
SmiMI CAYNNNNRTG 4 cut(s) 584, 1616, 2466, 3030
SmlI CTYRAG 7 cut(s) 131, 696, 1744, 1774, 2054, 2720, 3359
SmoI CTYRAG 7 cut(s) 131, 696, 1744, 1774, 2054, 2720, 3359
SphI GCATGC 1 cut(s) 670
SseBI AGGCCT 3 cut(s) 154, 1722, 3444
SsiI CCGC 7 cut(s) 42, 55, 328, 629, 670, 877, 2548
SspI AATATT 3 cut(s) 1323, 1912, 3007
SspMI CTAG 5 cut(s) 6, 183, 782, 1382, 2789
SstI GAGCTC 1 cut(s) 354
StuI AGGCCT 3 cut(s) 154, 1722, 3444
StyD4I CCNGG 6 cut(s) 970, 1528, 2995, 3270, 3385, 3517
StyI CCWWGG 3 cut(s) 661, 1606, 2002
TaaI ACNGT 6 cut(s) 1061, 1142, 1464, 1515, 3080, 3347
TaiI ACGT 2 cut(s) 1540, 2582
TaqI TCGA 6 cut(s) 94, 697, 1755, 2540, 2704, 3207
TaqII GACCGA 1 cut(s) 747
TatI WGTACW 2 cut(s) 2810, 3115
TauI GCSGC 1 cut(s) 331
TscAI CASTG 3 cut(s) 2823, 3541, 3547
TseFI GTSAC 1 cut(s) 2653
TseI GCWGC 4 cut(s) 218, 443, 631, 860
Tsp45I GTSAC 1 cut(s) 2653
TspGWI ACGGA 5 cut(s) 535, 1158, 2258, 3110, 3404
TspRI CASTG 3 cut(s) 2823, 3541, 3547
Van91I CCANNNNNTGG 2 cut(s) 1612, 3136
Vha464I CTTAAG 1 cut(s) 1774
VpaK11BI GGWCC 2 cut(s) 968, 1708
VspI ATTAAT 1 cut(s) 2099
XbaI TCTAGA 1 cut(s) 182
XceI RCATGY 1 cut(s) 670
XcmI CCANNNNNNNNNTGG 3 cut(s) 817, 2000, 3032
XhoI CTCGAG 1 cut(s) 696
XmiI GTMKAC 2 cut(s) 1543, 2785
XmnI GAANNNNTTC 7 cut(s) 589, 801, 1071, 1503, 1597, 2942, 2979
XspI CTAG 5 cut(s) 6, 183, 782, 1382, 2789
Zsp2I ATGCAT 3 cut(s) 266, 1473, 2839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.