Rw1G004400

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
8414784 .. 8419407
4624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004400.1

Sequence Viewer

Length: 3963 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGACGTGTTTATAAGTTTCAGCGGGGAAGATACCCGCAATACTTTTACCAGTTTTCTGTATTCTGCACTAAATCTGAGAGGAATTCTCACCTTTCTGGATGACCGAGAGCTTGAGAAAGGAAAATCCATTAGCTCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATCTGCGATTGTCATTCTCTCACCCAACTATGCTCGTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGACATGGGCCAACAAGTCCTCCCCGTCTTCTACGACGTGGATCCTTCTGATGTGCGGCACCAAAGGGGACATTTTGAGCTCAAAAGGAAAACCCAAGTAGATGTCGAAGTAAGGGAACATGAAGAAGTTTATGGCAAGAATGATGAGGACAGACTAAGTGCGTGGAGAGCTGCTTTGACTGAAGTCGCCAATCTTTCTGGCTGGGATTTCAAGAATTATTCATATGACGCAAGAGTAATTAACGAAATTGTTAATCATATACTAAGGAAGTCCGCGTATACATTTTCAAGTGTTTTCAAGGGCTTAATAGGAATGGATTCCCGCATTCATGATTTATTAAGCAATTACATATGTCCGCACCTTCGTAGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATTGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATTCGCCAGGATTTTGACCGAGCCTACTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTTCAAGAAAAACTTCTTTCCGAAATCCTAATGGCAAAAATTAAAAATATAGAGCATGAAGACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGAGACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATCATCATAACCACCACAGATATTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAGGGCCTTTAAGAAATGTCCCCCACCAGAAGATTATTTGCATCTGTGCTACGATATTATAGGGTATGCTAAGGGGCTTCCGTTGGCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGAACTGATGAGTGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGATGTGCTTCGGATTAGTTTTGATGGACTTGATGAAAAAGACAGAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGAGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACAAAATGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAAAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATAAACAATGTGCTGAAGAAAAATATGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTTGTAATGTGGACCTTCCTGACGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTGAGATCTCTCCCACAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGCTGAAGTTCATCAAACTCTGCCATTCTCAAAAAATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTGCAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAATGTTTGTTGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGATTCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTACGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATTAATAAATTGAAGTCTATTGAAGATCTTAATCTTTCTGGATGCTTGAACCTCGGCAAACCTCAGGTAAATGTGGGGGTGATGGACTGTTTTGAGGAAACTGATGTGAGTAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCGCAAGAAAGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCGTCTACCCATGATCGCAAGAAAAAAGGGAGATGTCCAATCTTTCAAGGATGCAAGGTAGTTTGGCGATCTTTCAAAAAGTTCTTGCCTTCTGGATTGGTGCGAAAAGTGAATACAAAGCCAAAGAGCTTCCACTTGCTTATATCAGAAAACGTGAATGCAGAGCCAAAGAGCTTACACGTGCTTATATCACAAAAAGTGAATGCAAAGCCAAAGAGTTTCCACTCGCCTATATCACAAAAAGTGAATGCAGAGCCAGAGAGTTTCCAGTCGCCTATATCACAAAACGTGAATGCAGAGCCACAGAGCTTCCACTCGCCTATATCACAAAACGTGAATGCAGAGCCAAAGAGCTTCCACTGGCCTATATCACAAAAAGTGAATACAGAGCCAAAGAGCTTCCACTTGCCTATATCACAAAAAGTGAATACAGAGCCAATGAGTTTCTGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAATCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAGCGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGGAACAATTTTGTTCGTCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAACAGCAATCAAGGATGCAATAACATAGCATTCGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAGCAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCTTGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCTGTAAGTGTATCTCTACCTGCACATTGTAATAACAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTCCATGAACACCATCAGGTGGATAAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTTGATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTAGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATGTGCAAGAGTTGAACAAAACAACCACTCAATCAAGTAGTGGGATGTCTCCTTACGAGGATATATTGATTAGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGGCAGTGGTAGCAGAACTTGCACGCTTGAAGAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1320

Amino Acids

149.71

Weight (kDa)

6.77

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 196 2e-53 TIR domain
TIR_2 PF13676 14 - 123 7.8e-17 TIR domain
NB-ARC PF00931 219 - 372 1.9e-18 NB-ARC domain
NACHT PF05729 222 - 318 2.4e-07 NACHT domain
WHD_ROQ1 PF23282 439 - 510 2.5e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 608 - 626 3.6e-06 Leucine Rich Repeat
LRR_13 PF23286 699 - 783 9.6e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 710 - 778 1.8e-09 Leucine-rich repeat region
C-JID PF20160 1127 - 1276 6.3e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 47, 3961
AarI CACCTGC 1 cut(s) 1937
Acc36I ACCTGC 2 cut(s) 1937, 3457
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1624, 3523
AccI GTMKAC 3 cut(s) 563, 2492, 3172
AccII CGCG 1 cut(s) 560
AciI CCGC 8 cut(s) 57, 70, 340, 558, 607, 641, 682, 889
AclWI GGATC 6 cut(s) 320, 333, 664, 677, 939, 1383
AcsI RAATTY 9 cut(s) 117, 726, 1681, 1804, 1879, 3017, 3229, 3545, 3650
AcuI CTGAAG 7 cut(s) 486, 1610, 1913, 3066, 3344, 3608, 3800
AcvI CACGTG 1 cut(s) 2668
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 3199, 3504, 3715
AfiI CCNNNNNNNGG 6 cut(s) 652, 1421, 1624, 2948, 3523, 3911
AflIII ACRYGT 2 cut(s) 39, 2665
AjiI CACGTC 2 cut(s) 40, 322
AjnI CCWGG 5 cut(s) 732, 982, 1540, 3382, 3772
AjuI GAANNNNNNNTTGG 2 cut(s) 3104, 3136
Alw21I GWGCWC 1 cut(s) 366
AlwI GGATC 6 cut(s) 320, 333, 664, 677, 939, 1383
AlwNI CAGNNNCTG 2 cut(s) 872, 2074
Ama87I CYCGRG 2 cut(s) 708, 2147
AoxI GGCC 7 cut(s) 292, 790, 1104, 1732, 2239, 2849, 3770
ApeKI GCWGC 3 cut(s) 182, 455, 872
ApoI RAATTY 9 cut(s) 117, 726, 1681, 1804, 1879, 3017, 3229, 3545, 3650
AseI ATTAAT 2 cut(s) 2111, 2298
Asp700I GAANNNNTTC 7 cut(s) 601, 813, 1083, 1515, 1609, 3329, 3366
AspS9I GGNCC 5 cut(s) 292, 980, 1104, 1720, 3770
AsuHPI GGTGA 7 cut(s) 115, 213, 2386, 3008, 3052, 3665, 3796
AsuII TTCGAA 1 cut(s) 3315
AvaI CYCGRG 2 cut(s) 708, 2147
AvaII GGWCC 2 cut(s) 980, 1720
AxyI CCTNAGG 1 cut(s) 2358
BamHI GGATCC 2 cut(s) 325, 669
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BbrPI CACGTG 1 cut(s) 2668
BbsI GAAGAC 7 cut(s) 304, 867, 1578, 1691, 2276, 3068, 3332
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 169, 442, 859
BccI CCATC 8 cut(s) 1301, 2013, 2371, 3169, 3185, 3420, 3525, 3590
BceAI ACGGC 2 cut(s) 1747, 2246
BciT130I CCWGG 5 cut(s) 734, 984, 1542, 3384, 3774
BciVI GTATCC 1 cut(s) 1791
BclI TGATCA 1 cut(s) 721
BfaI CTAG 5 cut(s) 198, 794, 1394, 3176, 3696
BfmI CTRYAG 2 cut(s) 2066, 2266
BfuAI ACCTGC 2 cut(s) 1937, 3457
BfuI GTATCC 1 cut(s) 1791
BglII AGATCT 5 cut(s) 200, 1445, 1790, 1972, 2320
BisI GCNGC 4 cut(s) 183, 341, 456, 873
BlsI GCNGC 4 cut(s) 184, 342, 457, 874
Bme1390I CCNGG 5 cut(s) 734, 984, 1542, 3384, 3774
Bme18I GGWCC 2 cut(s) 980, 1720
BmeT110I CYCGRG 2 cut(s) 708, 2147
BmgBI CACGTC 2 cut(s) 40, 322
BmgT120I GGNCC 5 cut(s) 292, 980, 1104, 1720, 3770
BmiI GGNNCC 3 cut(s) 327, 344, 671
BmrFI CCNGG 5 cut(s) 734, 984, 1542, 3384, 3774
BmrI ACTGGG 1 cut(s) 1785
BmsI GCATC 8 cut(s) 1033, 1150, 1470, 2327, 2528, 3211, 3233, 3287
BmuI ACTGGG 1 cut(s) 1785
BoxI GACNNNNGTC 1 cut(s) 467
BpiI GAAGAC 7 cut(s) 304, 867, 1578, 1691, 2276, 3068, 3332
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu10I CCTNAGC 1 cut(s) 1170
Bpu14I TTCGAA 1 cut(s) 3315
BpuEI CTTGAG 6 cut(s) 167, 1777, 1807, 1982, 3128, 3419
BsaAI YACGTR 1 cut(s) 2668
BsaBI GATNNNNATC 3 cut(s) 930, 1374, 2325
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1569, 1925
BsaJI CCNNGG 5 cut(s) 673, 983, 1618, 2014, 2347
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 6 cut(s) 652, 1421, 1624, 2948, 3523, 3911
Bse1I ACTGG 7 cut(s) 84, 977, 1780, 2755, 2852, 3905, 3921
Bse21I CCTNAGG 1 cut(s) 2358
Bse8I GATNNNNATC 3 cut(s) 930, 1374, 2325
BseBI CCWGG 5 cut(s) 734, 984, 1542, 3384, 3774
BseDI CCNNGG 5 cut(s) 673, 983, 1618, 2014, 2347
BseGI GGATG 8 cut(s) 139, 259, 1485, 1988, 2342, 2543, 3302, 3870
BseJI GATNNNNATC 3 cut(s) 930, 1374, 2325
BseLI CCNNNNNNNGG 6 cut(s) 652, 1421, 1624, 2948, 3523, 3911
BseMII CTCAG 4 cut(s) 101, 2225, 2372, 2969
BseNI ACTGG 7 cut(s) 84, 977, 1780, 2755, 2852, 3905, 3921
BseRI GAGGAG 2 cut(s) 206, 1754
BseXI GCAGC 3 cut(s) 169, 442, 859
BseYI CCCAGC 2 cut(s) 486, 1506
BsgI GTGCAG 2 cut(s) 84, 3435
Bsh1236I CGCG 1 cut(s) 560
BshFI GGCC 7 cut(s) 294, 792, 1106, 1734, 2241, 2851, 3772
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 2 cut(s) 708, 2147
BslFI GGGAC 2 cut(s) 366, 1104
BslI CCNNNNNNNGG 6 cut(s) 652, 1421, 1624, 2948, 3523, 3911
BsmFI GGGAC 2 cut(s) 366, 1104
BsmI GAATGC 7 cut(s) 609, 2650, 2695, 2740, 2785, 2830, 3311
BsnI GGCC 7 cut(s) 294, 792, 1106, 1734, 2241, 2851, 3772
Bso31I GGTCTC 2 cut(s) 1569, 1925
BsoBI CYCGRG 2 cut(s) 708, 2147
Bsp119I TTCGAA 1 cut(s) 3315
Bsp1286I GDGCHC 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 673
BspACI CCGC 8 cut(s) 57, 70, 340, 558, 607, 641, 682, 889
BspANI GGCC 7 cut(s) 294, 792, 1106, 1734, 2241, 2851, 3772
BspCNI CTCAG 4 cut(s) 102, 2226, 2371, 2970
BspFNI CGCG 1 cut(s) 560
BspHI TCATGA 2 cut(s) 613, 1567
BspLI GGNNCC 3 cut(s) 327, 344, 671
BspMAI CTGCAG 2 cut(s) 2070, 2270
BspMI ACCTGC 2 cut(s) 1937, 3457
BspPI GGATC 6 cut(s) 320, 333, 664, 677, 939, 1383
BspT104I TTCGAA 1 cut(s) 3315
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 2 cut(s) 1569, 1925
BsrI ACTGG 7 cut(s) 84, 977, 1780, 2755, 2852, 3905, 3921
BssECI CCNNGG 5 cut(s) 673, 983, 1618, 2014, 2347
BssNAI GTATAC 2 cut(s) 564, 3173
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 673, 1618, 2014
Bst1107I GTATAC 2 cut(s) 564, 3173
Bst2UI CCWGG 5 cut(s) 734, 984, 1542, 3384, 3774
Bst4CI ACNGT 5 cut(s) 1073, 1527, 2384, 3467, 3734
Bst6I CTCTTC 3 cut(s) 19, 807, 3595
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 4 cut(s) 1489, 2074, 3823, 3942
BstBAI YACGTR 1 cut(s) 2668
BstBI TTCGAA 1 cut(s) 3315
BstC8I GCNNGC 7 cut(s) 680, 1351, 1406, 2939, 3013, 3630, 3947
BstDSI CCRYGG 1 cut(s) 673
BstEII GGTNACC 1 cut(s) 3040
BstF5I GGATG 8 cut(s) 139, 259, 1485, 1988, 2342, 2543, 3302, 3870
BstFNI CGCG 1 cut(s) 560
BstMWI GCNNNNNNNGC 9 cut(s) 452, 684, 1489, 2074, 3103, 3491, 3823, 3933, 3942
BstNI CCWGG 5 cut(s) 734, 984, 1542, 3384, 3774
BstNSI RCATGY 1 cut(s) 682
BstPAI GACNNNNGTC 1 cut(s) 467
BstPI GGTNACC 1 cut(s) 3040
BstSCI CCNGG 5 cut(s) 732, 982, 1540, 3382, 3772
BstSFI CTRYAG 2 cut(s) 2066, 2266
BstUI CGCG 1 cut(s) 560
BstV1I GCAGC 3 cut(s) 169, 442, 859
BstV2I GAAGAC 7 cut(s) 304, 867, 1578, 1691, 2276, 3068, 3332
BstX2I RGATCY 7 cut(s) 200, 325, 669, 1445, 1790, 1972, 2320
BstYI RGATCY 7 cut(s) 200, 325, 669, 1445, 1790, 1972, 2320
BstZ17I GTATAC 2 cut(s) 564, 3173
Bsu36I CCTNAGG 1 cut(s) 2358
BsuI GTATCC 1 cut(s) 1791
BsuRI GGCC 7 cut(s) 294, 792, 1106, 1734, 2241, 2851, 3772
BtgI CCRYGG 1 cut(s) 673
BtrI CACGTC 2 cut(s) 40, 322
BtsCI GGATG 8 cut(s) 139, 259, 1485, 1988, 2342, 2543, 3302, 3870
BtsI GCAGTG 1 cut(s) 3934
BtsIMutI CAGTG 4 cut(s) 2845, 3203, 3928, 3934
BveI ACCTGC 2 cut(s) 1937, 3457
Cac8I GCNNGC 7 cut(s) 680, 1351, 1406, 2939, 3013, 3630, 3947
CaiI CAGNNNCTG 2 cut(s) 872, 2074
CciI TCATGA 2 cut(s) 613, 1567
Cfr13I GGNCC 5 cut(s) 292, 980, 1104, 1720, 3770
CseI GACGC 1 cut(s) 521
Csp6I GTAC 3 cut(s) 3198, 3503, 3714
CviQI GTAC 3 cut(s) 3198, 3503, 3714
Eam1104I CTCTTC 3 cut(s) 19, 807, 3595
EarI CTCTTC 3 cut(s) 19, 807, 3595
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 673, 1618, 2014
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 2 cut(s) 1569, 1925
Eco47I GGWCC 2 cut(s) 980, 1720
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 7 cut(s) 486, 1610, 1913, 3066, 3344, 3608, 3800
Eco72I CACGTG 1 cut(s) 2668
Eco81I CCTNAGG 1 cut(s) 2358
Eco88I CYCGRG 2 cut(s) 708, 2147
Eco91I GGTNACC 1 cut(s) 3040
EcoICRI GAGCTC 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 1104
EcoO65I GGTNACC 1 cut(s) 3040
EcoRI GAATTC 2 cut(s) 117, 3545
EcoRII CCWGG 5 cut(s) 732, 982, 1540, 3382, 3772
EcoT14I CCWWGG 3 cut(s) 673, 1618, 2014
EcoT22I ATGCAT 3 cut(s) 281, 1485, 3226
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 673, 1618, 2014
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FaqI GGGAC 2 cut(s) 366, 1104
FauI CCCGC 4 cut(s) 50, 77, 614, 675
FauNDI CATATG 2 cut(s) 508, 635
FbaI TGATCA 1 cut(s) 721
FblI GTMKAC 3 cut(s) 563, 2492, 3172
Fnu4HI GCNGC 4 cut(s) 183, 341, 456, 873
FokI GGATG 8 cut(s) 146, 266, 1492, 1995, 2349, 2550, 3309, 3877
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 4 cut(s) 183, 341, 456, 873
FspBI CTAG 5 cut(s) 198, 794, 1394, 3176, 3696
GluI GCNGC 4 cut(s) 183, 341, 456, 873
GsaI CCCAGC 2 cut(s) 490, 1510
HaeIII GGCC 7 cut(s) 294, 792, 1106, 1734, 2241, 2851, 3772
HgaI GACGC 1 cut(s) 521
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 9, 1638
HindII GTYRAC 2 cut(s) 9, 1638
HindIII AAGCTT 4 cut(s) 2030, 3104, 3530, 3590
HphI GGTGA 7 cut(s) 115, 213, 2386, 3008, 3052, 3665, 3796
Hpy166II GTNNAC 7 cut(s) 9, 564, 1523, 1638, 1720, 2493, 3173
Hpy8I GTNNAC 7 cut(s) 9, 564, 1523, 1638, 1720, 2493, 3173
Hpy99I CGWCG 3 cut(s) 24, 41, 323
HpyCH4III ACNGT 5 cut(s) 1073, 1527, 2384, 3467, 3734
HpyCH4IV ACGT 7 cut(s) 39, 321, 1549, 2640, 2667, 2775, 2820
HpyF10VI GCNNNNNNNGC 9 cut(s) 452, 684, 1489, 2074, 3103, 3491, 3823, 3933, 3942
HpySE526I ACGT 7 cut(s) 39, 321, 1549, 2640, 2667, 2775, 2820
Hsp92I GRCGYC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 721
LmnI GCTCC 2 cut(s) 869, 3002
Lsp1109I GCAGC 3 cut(s) 169, 442, 859
LweI GCATC 8 cut(s) 1033, 1150, 1470, 2327, 2528, 3211, 3233, 3287
MaeI CTAG 5 cut(s) 198, 794, 1394, 3176, 3696
MaeII ACGT 7 cut(s) 39, 321, 1549, 2640, 2667, 2775, 2820
MaeIII GTNAC 3 cut(s) 1381, 3023, 3040
MfeI CAATTG 3 cut(s) 186, 2223, 3268
MflI RGATCY 7 cut(s) 200, 325, 669, 1445, 1790, 1972, 2320
MhlI GDGCHC 1 cut(s) 366
MlyI GAGTC 4 cut(s) 203, 1755, 2080, 3807
MmeI TCCRAC 2 cut(s) 1488, 2137
Mph1103I ATGCAT 3 cut(s) 281, 1485, 3226
MroXI GAANNNNTTC 7 cut(s) 601, 813, 1083, 1515, 1609, 3329, 3366
MslI CAYNNNNRTG 2 cut(s) 1628, 3417
MspA1I CMGCKG 1 cut(s) 57
MspR9I CCNGG 5 cut(s) 734, 984, 1542, 3384, 3774
MunI CAATTG 3 cut(s) 186, 2223, 3268
Mva1269I GAATGC 7 cut(s) 609, 2650, 2695, 2740, 2785, 2830, 3311
MvaI CCWGG 5 cut(s) 734, 984, 1542, 3384, 3774
MvnI CGCG 1 cut(s) 560
MwoI GCNNNNNNNGC 9 cut(s) 452, 684, 1489, 2074, 3103, 3491, 3823, 3933, 3942
NcoI CCATGG 1 cut(s) 673
NdeI CATATG 2 cut(s) 508, 635
NlaIV GGNNCC 3 cut(s) 327, 344, 671
NmeAIII GCCGAG 1 cut(s) 2328
NmuCI GTSAC 2 cut(s) 1381, 3040
NsiI ATGCAT 3 cut(s) 281, 1485, 3226
NspI RCATGY 1 cut(s) 682
NspV TTCGAA 1 cut(s) 3315
PaeI GCATGC 1 cut(s) 682
PaeR7I CTCGAG 1 cut(s) 708
PagI TCATGA 2 cut(s) 613, 1567
PaqCI CACCTGC 1 cut(s) 1937
PcsI WCGNNNNNNNCGW 1 cut(s) 655
PctI GAATGC 7 cut(s) 609, 2650, 2695, 2740, 2785, 2830, 3311
PdmI GAANNNNTTC 7 cut(s) 601, 813, 1083, 1515, 1609, 3329, 3366
PflMI CCANNNNNTGG 2 cut(s) 1624, 3523
PfoI TCCNGGA 1 cut(s) 3382
PkrI GCNGC 4 cut(s) 184, 342, 457, 874
PleI GAGTC 4 cut(s) 202, 1755, 2079, 3806
PmaCI CACGTG 1 cut(s) 2668
PmlI CACGTG 1 cut(s) 2668
PpsI GAGTC 4 cut(s) 202, 1755, 2079, 3806
Ppu21I YACGTR 1 cut(s) 2668
PshAI GACNNNNGTC 1 cut(s) 467
PshBI ATTAAT 2 cut(s) 2111, 2298
PsiI TTATAA 2 cut(s) 47, 3961
Psp124BI GAGCTC 1 cut(s) 366
Psp6I CCWGG 5 cut(s) 732, 982, 1540, 3382, 3772
PspCI CACGTG 1 cut(s) 2668
PspEI GGTNACC 1 cut(s) 3040
PspFI CCCAGC 2 cut(s) 486, 1506
PspGI CCWGG 5 cut(s) 732, 982, 1540, 3382, 3772
PspN4I GGNNCC 3 cut(s) 327, 344, 671
PspPI GGNCC 5 cut(s) 292, 980, 1104, 1720, 3770
PspXI VCTCGAGB 1 cut(s) 708
PstI CTGCAG 2 cut(s) 2070, 2270
PstNI CAGNNNCTG 2 cut(s) 872, 2074
PsuI RGATCY 7 cut(s) 200, 325, 669, 1445, 1790, 1972, 2320
RsaI GTAC 3 cut(s) 3199, 3504, 3715
RsaNI GTAC 3 cut(s) 3198, 3503, 3714
RseI CAYNNNNRTG 2 cut(s) 1628, 3417
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 4 cut(s) 183, 341, 456, 873
Sau96I GGNCC 5 cut(s) 292, 980, 1104, 1720, 3770
SchI GAGTC 4 cut(s) 203, 1755, 2080, 3807
ScrFI CCNGG 5 cut(s) 734, 984, 1542, 3384, 3774
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 8 cut(s) 1033, 1150, 1470, 2327, 2528, 3211, 3233, 3287
SfcI CTRYAG 2 cut(s) 2066, 2266
Sfr274I CTCGAG 1 cut(s) 708
SfuI TTCGAA 1 cut(s) 3315
SinI GGWCC 2 cut(s) 980, 1720
SlaI CTCGAG 1 cut(s) 708
SmiMI CAYNNNNRTG 2 cut(s) 1628, 3417
SmlI CTYRAG 7 cut(s) 146, 708, 1756, 1786, 1961, 3107, 3398
SmoI CTYRAG 7 cut(s) 146, 708, 1756, 1786, 1961, 3107, 3398
SphI GCATGC 1 cut(s) 682
SsiI CCGC 8 cut(s) 57, 70, 340, 558, 607, 641, 682, 889
SspI AATATT 2 cut(s) 1335, 3394
SspMI CTAG 5 cut(s) 198, 794, 1394, 3176, 3696
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 5 cut(s) 732, 982, 1540, 3382, 3772
StyI CCWWGG 3 cut(s) 673, 1618, 2014
TaaI ACNGT 5 cut(s) 1073, 1527, 2384, 3467, 3734
TaiI ACGT 7 cut(s) 42, 324, 1552, 2643, 2670, 2778, 2823
TaqI TCGA 5 cut(s) 390, 709, 1378, 1767, 3315
TaqII GACCGA 2 cut(s) 153, 759
TatI WGTACW 2 cut(s) 3197, 3502
TauI GCSGC 1 cut(s) 343
TscAI CASTG 4 cut(s) 2852, 3210, 3928, 3934
TseFI GTSAC 2 cut(s) 1381, 3040
TseI GCWGC 3 cut(s) 182, 455, 872
Tsp45I GTSAC 2 cut(s) 1381, 3040
TspGWI ACGGA 2 cut(s) 1170, 3791
TspRI CASTG 4 cut(s) 2852, 3210, 3928, 3934
Van91I CCANNNNNTGG 2 cut(s) 1624, 3523
VpaK11BI GGWCC 2 cut(s) 980, 1720
VspI ATTAAT 2 cut(s) 2111, 2298
XapI RAATTY 9 cut(s) 117, 726, 1681, 1804, 1879, 3017, 3229, 3545, 3650
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 682
XcmI CCANNNNNNNNNTGG 2 cut(s) 2012, 3419
XhoI CTCGAG 1 cut(s) 708
XmiI GTMKAC 3 cut(s) 563, 2492, 3172
XmnI GAANNNNTTC 7 cut(s) 601, 813, 1083, 1515, 1609, 3329, 3366
XspI CTAG 5 cut(s) 198, 794, 1394, 3176, 3696
Zsp2I ATGCAT 3 cut(s) 281, 1485, 3226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.