Rroxscaffold_4G00326770

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
58978921 .. 58984654
5734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326770.1

Sequence Viewer

Length: 2265 bp
ATGACCTGCATAAAAAAAGAGAAAGAAATATTCCTTCATATTGCTTGCTTTTACAAGGGGATGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTTCAACCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTGTCTCCAACAATGATTTGTCGATGCATGATTTGCTACAAGAAATGGGCCGGGAAATTGTTCGTGAACAGTCTCCTGATGAGCCAGGCAAATGTAGTAGATTATGGTCTCATAAAGACATCAACAAGGTGCTGGAGAGAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTGAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTATTCTTCTCCATATTCGTAATGTCGACCTTCCGGATGGCCTTACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTTCCACAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTCTGCCGCAGCAACATTAAACAGCTTTGGAAGGGGACAAAGAATTTTGACAAGTTGAAATTAATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCACAGGTGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTAAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCATGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTCAGTGCTTTGTCTTGATGAGACTGCCATTGAGGAATTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTATGTCTTCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAATATCAGGTAAATGTAGGGGAGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATTGAAATGTCATCTACCCATGGTCTCATAAAATATGAGAGAGGTTCAAACTTTCATGGATGCGAAGTAGTTTTGGAATCTTTAAATGAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAATGAGTTTCCGCTTGCCTATATCTGGTCTCTGTAATTTAACATATCTGAATCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAATGAATTTGGTTACTTTCCCTCTTTAGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCATTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGACTAAAGAGATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATGAAGGATGCAATACCATAGCATTTGAAATGCTGAGGACACTCATGTATCAGGGAATCTCTAATAAGAGGGAAACCTTTCAAATTGTAATTCCTGGAAGTAATATCCATGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCCTTAAGTGTATCTCTACCTGCACATTGGAATAAGAGTCGGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCGGTGGATGAGCTTTATGTAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTGGAAGTGGGTGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAGTCAGATCACCTGTGGCTATTCTATGTATCTTGTGATAAATACTTTGGTATAGAGAGGTGGCTTTGCGGTTTGAATCATCTAGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAAACAGTGTGGAATCCGTCTGATATATGAGCAAGATGTGCAAGAGTCGAACGAAACAATGCAAGAGTCGAACAAAACAACCACTCAATCAAGCAGTAGGATGTCTCTTATGAGTATATATGATTGGTTTCGACGTTTCAGCTACATGGGAAACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

754

Amino Acids

86.02

Weight (kDa)

5.77

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 6 - 75 2.2e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 236 - 343 5.1e-10 Leucine-rich repeat region
LRR_13 PF23286 264 - 348 2.8e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 567 - 716 1.3e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 14, 1777
AccB7I CCANNNNNTGG 2 cut(s) 319, 1843
AccI GTMKAC 2 cut(s) 414, 1492
AccIII TCCGGA 1 cut(s) 421
AciI CCGC 3 cut(s) 538, 1255, 2052
AclI AACGTT 1 cut(s) 865
AclWI GGATC 1 cut(s) 78
AcsI RAATTY 8 cut(s) 376, 499, 574, 685, 1337, 1549, 1865, 1970
AcuI CTGAAG 3 cut(s) 1386, 1928, 2120
AfaI GTAC 2 cut(s) 1519, 1824
AfiI CCNNNNNNNGG 4 cut(s) 116, 319, 1268, 1843
AflII CTTAAG 2 cut(s) 481, 1753
AjnI CCWGG 3 cut(s) 235, 1702, 1977
AjuI GAANNNNNNNTTGG 2 cut(s) 1424, 1456
AluBI AGCT 7 cut(s) 103, 556, 727, 1426, 1852, 1912, 2247
AluI AGCT 7 cut(s) 103, 556, 727, 1426, 1852, 1912, 2247
Alw21I GWGCWC 1 cut(s) 1836
AlwI GGATC 1 cut(s) 78
Ama87I CYCGRG 2 cut(s) 761, 842
Aor13HI TCCGGA 1 cut(s) 421
AoxI GGCC 4 cut(s) 199, 427, 934, 2090
ApeKI GCWGC 1 cut(s) 540
ApoI RAATTY 8 cut(s) 376, 499, 574, 685, 1337, 1549, 1865, 1970
AseI ATTAAT 2 cut(s) 593, 806
Asp700I GAANNNNTTC 3 cut(s) 210, 304, 1686
AspS9I GGNCC 2 cut(s) 199, 2090
AsuC2I CCSGG 2 cut(s) 203, 2094
AsuHPI GGTGA 3 cut(s) 1328, 1985, 2116
AvaI CYCGRG 2 cut(s) 761, 842
BaeI ACNNNNGTAYC 2 cut(s) 1640, 1673
BbsI GAAGAC 1 cut(s) 971
Bbv12I GWGCWC 1 cut(s) 1836
BbvCI CCTCAGC 1 cut(s) 1643
BbvI GCAGC 1 cut(s) 552
BccI CCATC 9 cut(s) 55, 419, 708, 998, 1066, 1489, 1505, 1740, 1845
BceAI ACGGC 1 cut(s) 941
BcgI CGANNNNNNTGC 2 cut(s) 742, 776
BciT130I CCWGG 3 cut(s) 237, 1704, 1979
BciVI GTATCC 1 cut(s) 486
BcnI CCSGG 2 cut(s) 203, 2094
BfaI CTAG 3 cut(s) 89, 1496, 2066
BfmI CTRYAG 1 cut(s) 961
BfrI CTTAAG 2 cut(s) 481, 1753
BfuAI ACCTGC 2 cut(s) 14, 1777
BfuI GTATCC 1 cut(s) 486
BglII AGATCT 3 cut(s) 140, 485, 667
BisI GCNGC 2 cut(s) 538, 541
BlsI GCNGC 2 cut(s) 539, 542
Bme1390I CCNGG 5 cut(s) 203, 237, 1704, 1979, 2094
BmeT110I CYCGRG 2 cut(s) 761, 842
BmgT120I GGNCC 2 cut(s) 199, 2090
BmrFI CCNGG 5 cut(s) 203, 237, 1704, 1979, 2094
BmrI ACTGGG 1 cut(s) 480
BmsI GCATC 6 cut(s) 165, 1022, 1163, 1531, 1553, 1607
BmuI ACTGGG 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 971
BpmI CTGGAG 1 cut(s) 305
Bpu10I CCTNAGC 1 cut(s) 1643
BpuEI CTTGAG 3 cut(s) 472, 677, 1448
BpuMI CCSGG 2 cut(s) 203, 2094
BsaBI GATNNNNATC 1 cut(s) 69
BsaI GGTCTC 4 cut(s) 264, 620, 1142, 1277
BsaJI CCNNGG 3 cut(s) 313, 709, 1132
BsaWI WCCGGW 2 cut(s) 421, 1840
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 319, 1268, 1843
Bse1I ACTGG 3 cut(s) 475, 937, 2264
Bse3DI GCAATG 1 cut(s) 1615
Bse8I GATNNNNATC 1 cut(s) 69
BseAI TCCGGA 1 cut(s) 421
BseBI CCWGG 3 cut(s) 237, 1704, 1979
BseDI CCNNGG 3 cut(s) 313, 709, 1132
BseGI GGATG 9 cut(s) 66, 430, 683, 1037, 1178, 1622, 1837, 1852, 2211
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 4 cut(s) 116, 319, 1268, 1843
BseMI GCAATG 1 cut(s) 1615
BseMII CTCAG 2 cut(s) 1289, 1634
BseNI ACTGG 3 cut(s) 475, 937, 2264
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 1 cut(s) 552
BsgI GTGCAG 1 cut(s) 1755
BshFI GGCC 4 cut(s) 201, 429, 936, 2092
BsiHKAI GWGCWC 1 cut(s) 1836
BsiHKCI CYCGRG 2 cut(s) 761, 842
BsiSI CCGG 4 cut(s) 202, 422, 1841, 2093
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 4 cut(s) 116, 319, 1268, 1843
BsmFI GGGAC 1 cut(s) 580
BsmI GAATGC 2 cut(s) 1395, 1407
BsnI GGCC 4 cut(s) 201, 429, 936, 2092
Bso31I GGTCTC 4 cut(s) 264, 620, 1142, 1277
BsoBI CYCGRG 2 cut(s) 761, 842
Bsp1286I GDGCHC 1 cut(s) 1836
Bsp13I TCCGGA 1 cut(s) 421
Bsp143I GATC 6 cut(s) 70, 140, 485, 667, 1568, 1990
Bsp19I CCATGG 1 cut(s) 1132
BspACI CCGC 3 cut(s) 538, 1255, 2052
BspANI GGCC 4 cut(s) 201, 429, 936, 2092
BspCNI CTCAG 2 cut(s) 1290, 1635
BspEI TCCGGA 1 cut(s) 421
BspMAI CTGCAG 1 cut(s) 965
BspMI ACCTGC 2 cut(s) 14, 1777
BspPI GGATC 1 cut(s) 78
BspTI CTTAAG 2 cut(s) 481, 1753
BspTNI GGTCTC 4 cut(s) 264, 620, 1142, 1277
BsrDI GCAATG 1 cut(s) 1615
BsrI ACTGG 3 cut(s) 475, 937, 2264
BssECI CCNNGG 3 cut(s) 313, 709, 1132
BssMI GATC 6 cut(s) 70, 140, 485, 667, 1568, 1990
BssT1I CCWWGG 3 cut(s) 313, 709, 1132
Bst2UI CCWGG 3 cut(s) 237, 1704, 1979
Bst4CI ACNGT 4 cut(s) 154, 222, 1079, 2112
Bst6I CTCTTC 2 cut(s) 495, 1915
BstAFI CTTAAG 2 cut(s) 481, 1753
BstAPI GCANNNNNTGC 2 cut(s) 184, 2143
BstC8I GCNNGC 4 cut(s) 46, 101, 1259, 1950
BstDEI CTNAG 5 cut(s) 953, 1298, 1373, 1422, 1643
BstDSI CCRYGG 1 cut(s) 1132
BstF5I GGATG 9 cut(s) 66, 430, 683, 1037, 1178, 1622, 1837, 1852, 2211
BstKTI GATC 6 cut(s) 73, 143, 488, 670, 1571, 1993
BstMBI GATC 6 cut(s) 70, 140, 485, 667, 1568, 1990
BstMWI GCNNNNNNNGC 4 cut(s) 184, 1423, 1811, 2143
BstNI CCWGG 3 cut(s) 237, 1704, 1979
BstSCI CCNGG 5 cut(s) 201, 235, 1702, 1977, 2092
BstSFI CTRYAG 1 cut(s) 961
BstV1I GCAGC 1 cut(s) 552
BstV2I GAAGAC 1 cut(s) 971
BstX2I RGATCY 3 cut(s) 140, 485, 667
BstYI RGATCY 3 cut(s) 140, 485, 667
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 4 cut(s) 201, 429, 936, 2092
BtgI CCRYGG 1 cut(s) 1132
BtsCI GGATG 9 cut(s) 66, 430, 683, 1037, 1178, 1622, 1837, 1852, 2211
BtsIMutI CAGTG 3 cut(s) 879, 1523, 2117
BveI ACCTGC 2 cut(s) 14, 1777
Cac8I GCNNGC 4 cut(s) 46, 101, 1259, 1950
Cfr13I GGNCC 2 cut(s) 199, 2090
Csp6I GTAC 2 cut(s) 1518, 1823
CviAII CATG 9 cut(s) 179, 319, 774, 1133, 1169, 1654, 1718, 1829, 2251
CviQI GTAC 2 cut(s) 1518, 1823
DdeI CTNAG 5 cut(s) 953, 1298, 1373, 1422, 1643
DpnI GATC 6 cut(s) 72, 142, 487, 669, 1570, 1992
DpnII GATC 6 cut(s) 70, 140, 485, 667, 1568, 1990
DraI TTTAAA 1 cut(s) 1197
Eam1104I CTCTTC 2 cut(s) 495, 1915
EarI CTCTTC 2 cut(s) 495, 1915
Eco130I CCWWGG 3 cut(s) 313, 709, 1132
Eco31I GGTCTC 4 cut(s) 264, 620, 1142, 1277
Eco57I CTGAAG 3 cut(s) 1386, 1928, 2120
Eco88I CYCGRG 2 cut(s) 761, 842
EcoRI GAATTC 1 cut(s) 1865
EcoRII CCWGG 3 cut(s) 235, 1702, 1977
EcoT14I CCWWGG 3 cut(s) 313, 709, 1132
EcoT22I ATGCAT 2 cut(s) 180, 1546
ErhI CCWWGG 3 cut(s) 313, 709, 1132
FaeI CATG 9 cut(s) 182, 322, 777, 1136, 1172, 1657, 1721, 1832, 2254
FaqI GGGAC 1 cut(s) 580
FatI CATG 9 cut(s) 178, 318, 773, 1132, 1168, 1653, 1717, 1828, 2250
FblI GTMKAC 2 cut(s) 414, 1492
Fnu4HI GCNGC 2 cut(s) 538, 541
FokI GGATG 9 cut(s) 73, 437, 690, 1044, 1185, 1629, 1824, 1859, 2218
Fsp4HI GCNGC 2 cut(s) 538, 541
FspBI CTAG 3 cut(s) 89, 1496, 2066
GluI GCNGC 2 cut(s) 538, 541
GsuI CTGGAG 1 cut(s) 305
HaeIII GGCC 4 cut(s) 201, 429, 936, 2092
HapII CCGG 4 cut(s) 202, 422, 1841, 2093
Hin1II CATG 9 cut(s) 182, 322, 777, 1136, 1172, 1657, 1721, 1832, 2254
HincII GTYRAC 2 cut(s) 333, 415
HindII GTYRAC 2 cut(s) 333, 415
HindIII AAGCTT 2 cut(s) 725, 1424
HpaII CCGG 4 cut(s) 202, 422, 1841, 2093
HphI GGTGA 3 cut(s) 1328, 1985, 2116
Hpy166II GTNNAC 4 cut(s) 218, 333, 415, 1493
Hpy188I TCNGA 8 cut(s) 651, 1293, 1299, 1474, 1737, 1990, 2100, 2127
Hpy8I GTNNAC 4 cut(s) 218, 333, 415, 1493
Hpy99I CGWCG 1 cut(s) 2241
HpyCH4III ACNGT 4 cut(s) 154, 222, 1079, 2112
HpyCH4IV ACGT 2 cut(s) 865, 2239
HpyF10VI GCNNNNNNNGC 4 cut(s) 184, 1423, 1811, 2143
HpyF3I CTNAG 5 cut(s) 953, 1298, 1373, 1422, 1643
HpySE526I ACGT 2 cut(s) 865, 2239
Hsp92II CATG 9 cut(s) 182, 322, 777, 1136, 1172, 1657, 1721, 1832, 2254
Kpn2I TCCGGA 1 cut(s) 421
Kzo9I GATC 6 cut(s) 70, 140, 485, 667, 1568, 1990
LmnI GCTCC 1 cut(s) 1322
Lsp1109I GCAGC 1 cut(s) 552
LweI GCATC 6 cut(s) 165, 1022, 1163, 1531, 1553, 1607
MaeI CTAG 3 cut(s) 89, 1496, 2066
MaeII ACGT 2 cut(s) 865, 2239
MaeIII GTNAC 3 cut(s) 76, 1343, 1360
MalI GATC 6 cut(s) 72, 142, 487, 669, 1570, 1992
MboI GATC 6 cut(s) 70, 140, 485, 667, 1568, 1990
MboII GAAGA 6 cut(s) 386, 482, 847, 971, 1932, 1979
MfeI CAATTG 3 cut(s) 918, 1113, 1588
MflI RGATCY 3 cut(s) 140, 485, 667
MhlI GDGCHC 1 cut(s) 1836
MlyI GAGTC 6 cut(s) 450, 775, 1795, 1994, 2159, 2180
MmeI TCCRAC 2 cut(s) 183, 832
Mph1103I ATGCAT 2 cut(s) 180, 1546
MroI TCCGGA 1 cut(s) 421
MroXI GAANNNNTTC 3 cut(s) 210, 304, 1686
MslI CAYNNNNRTG 2 cut(s) 323, 1737
MspCI CTTAAG 2 cut(s) 481, 1753
MspI CCGG 4 cut(s) 202, 422, 1841, 2093
MspR9I CCNGG 5 cut(s) 203, 237, 1704, 1979, 2094
MunI CAATTG 3 cut(s) 918, 1113, 1588
Mva1269I GAATGC 2 cut(s) 1395, 1407
MvaI CCWGG 3 cut(s) 237, 1704, 1979
MwoI GCNNNNNNNGC 4 cut(s) 184, 1423, 1811, 2143
NciI CCSGG 2 cut(s) 203, 2094
NcoI CCATGG 1 cut(s) 1132
NdeII GATC 6 cut(s) 70, 140, 485, 667, 1568, 1990
NlaIII CATG 9 cut(s) 182, 322, 777, 1136, 1172, 1657, 1721, 1832, 2254
NmeAIII GCCGAG 1 cut(s) 1023
NmuCI GTSAC 2 cut(s) 76, 1360
NsiI ATGCAT 2 cut(s) 180, 1546
PaeR7I CTCGAG 1 cut(s) 761
PctI GAATGC 2 cut(s) 1395, 1407
PdmI GAANNNNTTC 3 cut(s) 210, 304, 1686
PflMI CCANNNNNTGG 2 cut(s) 319, 1843
PfoI TCCNGGA 1 cut(s) 1702
PkrI GCNGC 2 cut(s) 539, 542
PleI GAGTC 6 cut(s) 450, 774, 1794, 1993, 2158, 2179
PpsI GAGTC 6 cut(s) 450, 774, 1794, 1993, 2158, 2179
PshBI ATTAAT 2 cut(s) 593, 806
Psp1406I AACGTT 1 cut(s) 865
Psp6I CCWGG 3 cut(s) 235, 1702, 1977
PspGI CCWGG 3 cut(s) 235, 1702, 1977
PspPI GGNCC 2 cut(s) 199, 2090
PstI CTGCAG 1 cut(s) 965
PsuI RGATCY 3 cut(s) 140, 485, 667
RsaI GTAC 2 cut(s) 1519, 1824
RsaNI GTAC 2 cut(s) 1518, 1823
RseI CAYNNNNRTG 2 cut(s) 323, 1737
SalI GTCGAC 1 cut(s) 413
SatI GCNGC 2 cut(s) 538, 541
Sau3AI GATC 6 cut(s) 70, 140, 485, 667, 1568, 1990
Sau96I GGNCC 2 cut(s) 199, 2090
SchI GAGTC 6 cut(s) 450, 775, 1795, 1994, 2159, 2180
ScrFI CCNGG 5 cut(s) 203, 237, 1704, 1979, 2094
SduI GDGCHC 1 cut(s) 1836
SfaNI GCATC 6 cut(s) 165, 1022, 1163, 1531, 1553, 1607
SfcI CTRYAG 1 cut(s) 961
Sfr274I CTCGAG 1 cut(s) 761
SlaI CTCGAG 1 cut(s) 761
SmiMI CAYNNNNRTG 2 cut(s) 323, 1737
SmlI CTYRAG 6 cut(s) 451, 481, 656, 761, 1427, 1753
SmoI CTYRAG 6 cut(s) 451, 481, 656, 761, 1427, 1753
SsiI CCGC 3 cut(s) 538, 1255, 2052
SspI AATATT 1 cut(s) 30
SspMI CTAG 3 cut(s) 89, 1496, 2066
StyD4I CCNGG 5 cut(s) 201, 235, 1702, 1977, 2092
StyI CCWWGG 3 cut(s) 313, 709, 1132
TaaI ACNGT 4 cut(s) 154, 222, 1079, 2112
TaiI ACGT 2 cut(s) 868, 2242
TaqI TCGA 8 cut(s) 173, 414, 462, 762, 1411, 2153, 2174, 2236
TatI WGTACW 2 cut(s) 1517, 1822
TauI GCSGC 1 cut(s) 540
TscAI CASTG 3 cut(s) 879, 1530, 2117
TseFI GTSAC 2 cut(s) 76, 1360
TseI GCWGC 1 cut(s) 540
Tsp45I GTSAC 2 cut(s) 76, 1360
TspDTI ATGAA 7 cut(s) 26, 528, 1157, 1350, 1573, 1626, 1878
TspGWI ACGGA 1 cut(s) 2111
TspRI CASTG 3 cut(s) 879, 1530, 2117
Van91I CCANNNNNTGG 2 cut(s) 319, 1843
Vha464I CTTAAG 2 cut(s) 481, 1753
VspI ATTAAT 2 cut(s) 593, 806
XapI RAATTY 8 cut(s) 376, 499, 574, 685, 1337, 1549, 1865, 1970
XbaI TCTAGA 1 cut(s) 2065
XcmI CCANNNNNNNNNTGG 2 cut(s) 707, 1739
XhoI CTCGAG 1 cut(s) 761
XmiI GTMKAC 2 cut(s) 414, 1492
XmnI GAANNNNTTC 3 cut(s) 210, 304, 1686
XspI CTAG 3 cut(s) 89, 1496, 2066
Zsp2I ATGCAT 2 cut(s) 180, 1546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.